Nucleic derivatives chromatography gel. Medical search. Frequent questions

The purity of the compounds was monitored by thin layer chromatography (TLC) and the structures of the products were confirmed by elemental and spectral analysis. (ijpsonline.com)
Precoated silica gel 60 F 254 plates (Merck) for thin layer chromatography (TLC) were used. (hindawi.com)

An extensive human sperm proteome analysis using 1D-SDS-PAGE combined with electrospray liquid chromatography tandem mass spectrometry (GeLC-MS/ MS) approach identified 1,760 proteins (24). (jri.ir)
1984. Application of high-performance liquid chromatography with synchronized accumulating radioisotope detector to analysis of glyceryl trinitrate and its metabolites in rat plasma. (cdc.gov)

Enzymes such as ammonialyases, methylases and hydroxylases then transform these amino acids to cinnamic acid derivatives which undergo o-hydroxylation yielding coumarins. (wikipedia.org)
To understand the function of RBPs and the mechanism by which RBP-mediated posttranscriptional regulation of gene expression in plants under various environmental stresses, the gene families encoding glycine rich-RBP (GR-RBP), zinc finger-RBP, and cold shock domain-RBP (CSD-RBP) were characterized for their nucleic acid-binding property, expression pattern and function in stress responses in Arabidopsis thaliana. (khugene.com)
Nucleic acid-binding capabilities of the RBPs vary depending on the types of RBPs. (khugene.com)
periodate-oxidized ribonucleotides can subsequently be converted to fluorescent nucleic acid probes by reaction with fluorescent hydrazines, hydroxylamines and amines. (thermofisher.com)
Here we report a directed evolution strategy for discovering polymerases that can synthesize threose nucleic acid (TNA) on DNA templates. (bvsalud.org)
Here, we describe the evolution of a biologically stable artificial genetic system composed of α-l-threofuranosyl nucleic acid (TNA) that facilitates the production of backbone- and base-modified aptamers termed "threomers" that function as high quality protein capture reagents. (bvsalud.org)
Here, we compare the substrate specificity, thermal stability, reverse transcriptase activity, and fidelity of laboratory-evolved polymerases that were established to synthesize RNA, 2'-fluoroarabino nucleic acid (FANA), arabino nucleic acid (ANA), hexitol nucleic acid (HNA), threose nucleic acid (TNA), and phosphonomethylthreosyl nucleic acid (PMT). (bvsalud.org)
α-L-Threose nucleic acid (TNA), a type of XNA that is recalcitrant to nuclease digestion and amenable to Darwinian evolution, provides a model system for developing XNAs with functional groups that are not present in natural DNA and RNA. (bvsalud.org)
These 'guide' crRNAs are incorporated into a ribonucleoprotein interference complex that can recognize and cleave its complementary target sequence, thus destroying the invading nucleic acid. (elifesciences.org)

MALDI analysis of proteins after extraction from dissolvable ethylene glycol diacrylate cross-linked polyacrylamide gels. (uni-frankfurt.de)
Techniques used in human sperm proteome mapping: There are several initial reports using MS proteomics technology to identify a limited number of proteins from the human sperm using twodimensional gel electrophoresis (2-DE) coupled to MALDI-TOF-MS analysis (16 - 23). (jri.ir)
The inner fluid can contain cargo molecules such as nucleic acids or soluble proteins. (biomedcentral.com)

Dextran and its derivative CM-Dextran have a long history as biocompatible materials and the crosslinked form of CMD (Sephadex®) has been used for more than 50 years as a stationary phase for chromatography of biomolecules. (xantec.com)

Their molecular backbones are composed of repeating units of saccharide, nucleic acids, or amino acids and sometimes various additional side chains contributing also to their functionalities. (benthambooks.com)

Effect of metallic cations on human serum: Study by starch-gel electrophoresis. (cdc.gov)

Isatin (1-H-indole-2,3- dione) and its derivatives possess diverse biological and pharmacological activities and are widely used as a starting material for the synthesis of a broad range of heterocyclic compound substrates for drug synthesis [ 1 ]. (ijpsonline.com)
The synthesis of the ribo - trihydroxy sugar derivative starting from diacetone-D-glucose led to the formation of an inseparable 1:1 mixture of the required compound and its C-3 epimer, i.e., 4- C- hydroxymethyl-1,2- O -isopropylidene-α-D-xylofuranose [10] . (beilstein-journals.org)

Alkenes from unsaturated fatty acids and ceramides can also be converted to glycols by osmium tetroxide and then oxidized by periodate to aldehydes, and periodate will oxidize certain β-aminoethanol derivatives such as the hydroxylysine residues in collagen, as well as methionine (to its sulfoxide) and certain thiols (usually to disulfides). (thermofisher.com)

A rapid and highly efficient purification protocol using chromatography on heparin affinity, hydroxyapatite, and gel filtration media allows for the recovery of large quantities of the recombinant enzyme, which is judged to be greater than 98% homogenous by SDS/PAGE. (rhea-db.org)

Synthetic genetic polymers (xeno-nucleic acids, XNAs) have the potential to transition aptamers from laboratory tools to therapeutic agents, but additional functionality is needed to compete with antibodies. (bvsalud.org)
Synthetic genetics is an area of synthetic biology that aims to extend the properties of heredity and evolution to artificial genetic polymers, commonly known as xeno-nucleic acids or XNAs. (bvsalud.org)
Non-natural oligonucleotides are valuable probes of biological systems, as they can convey synthetic control of endogenous nucleic acids with high sequence specificity. (cdc.gov)

However, natural polymerases do not recognize artificial genetic polymers (also known as xeno-nucleic acids or XNAs) with unique sugar-phosphate backbone structures. (bvsalud.org)

These introduced aldehydes and ketones can then be modified with fluorescent or biotinylated hydrazine, hydroxylamine or amine derivatives to label the polysaccharide or glycoprotein. (thermofisher.com)

Several investigations suggest that, besides their extracellular actions, also intracellular mechanisms of sGAG-derivatives seem possible. (degruyter.com)
Alginate hydrogels are particularly attractive in wound healing, drug delivery, and tissue engineering applications, as these gels retain structural similarity to the extracellular matrices in tissues. (xantec.com)

Additionally, in the isogenic derivative with the inactivated tnaA gene, no indole was detected and acetate and lactate were accumulated. (biomedcentral.com)
Phosphorodiamidate morpholino oligonucleotides are widely used to interrogate gene function in whole organisms, and light-activatable derivatives can reveal spatial and temporal differences in gene activity. (cdc.gov)

The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. (lookformedical.com)

Imidazole derivatives are widely found in nature, such as histamine, histidine and benzimidazole, etc. (lookchem.com)

Consecutive pathway and cluster analysis led to the identification of biological processes, namely processes involving binding and processing of nucleic acids, LRP1-dependent endocytosis, and exosome formation. (degruyter.com)
The effect of the psoralen derivatives from radix Angelicae dahuricae on retinoid X receptor- α enzymes transcriptional regulation was reported [ 4 ] and this in turn plays key roles in various biological processes including diabetes and obesity. (hindawi.com)

Two-dimensional gel and proteome analyses suggest several target genes modulated by each RBPs. (khugene.com)
A study of Escherichia coli physiology during growth on glycerol was performed combining transcriptional-proteomic analysis as well as kinetic and stoichiometric evaluations in the strain JM101 and certain derivatives with important inactivated genes. (biomedcentral.com)
In agreement, no acetate was detected when growing on glycerol, this strain was also capable of glycerol and acetate coutilization when growing in mineral media and derivatives carrying inactivated poxB or pckA genes, accumulated acetate. (biomedcentral.com)

A polyphenol oxidase catalyzing 3¢-hydroxylation of larreatricin to 3¢-hydroxylarreatricin was purified from the leaves of creosote bush by DEAE-Sepharose, Hydroxylapatite, and Phenyl Sepharose column chromatographies. (khugene.com)
Column chromatography (CC) was carried out using silica gel (Si) 60 mesh of 35-60 and 60-120 (E. Merck, Darmstadt, Germany). (hindawi.com)

Of which, 3¢-O-methyl-NDGA and methylated/ acetylated NDGA derivatives has antiviral activities against HIV, herpes simplex virus and/or human papilloma virus. (khugene.com)

Nucleic acids research online , 2022, str. (ki.si)
Nucleic Acids Research 2021 Jul 9;49(12):6880-6892. (uu.se)

Model-based process optimization for mAb chromatography. (ki.si)
Key to CRISPR-Cas adaptive immunity is maintaining an ongoing record of invading nucleic acids, a process carried out by the Cas1-Cas2 complex that integrates short segments of foreign genetic material (spacers) into the CRISPR locus. (elifesciences.org)

The structure of both the monoacetylated epimers, i.e., 5- O -acetyl-4- C -hydroxymethyl-1,2- O -isopropylidene-α-D-ribo- and xylofuranose obtained by enzymatic acetylation, has been confirmed by an X-ray study on their corresponding 4- C - p -toluenesulfonyloxymethyl derivatives. (beilstein-journals.org)

Enzymes such as ammonialyases, methylases and hydroxylases then transform these amino acids to cinnamic acid derivatives which undergo o-hydroxylation yielding coumarins. (wikipedia.org)
[0002] The invention relates to isolated nucleic acids and polypeptides derived from Staphylococcus epidermidis that are useful as molecular targets for diagnostics, prophylaxis and treatment of pathological conditions, as well as materials and methods for the diagnosis, prevention, and amelioration of pathological conditions resulting from bacterial infection. (patentsencyclopedia.com)
Since the isolation of nucleic acids to the mapping of human genome, chemical synthesis of nucleic acids has undergone tremendous advancements. (researchgate.net)
Further improvements in this area such as, introduction of high throughput synthesizers, better coupling reagents, improved polymer supports, newer sets of protecting groups for exocyclic amino groups of nucleic bases and introduction of universal polymer supports have completely revolutionized the entire field of nucleic acids chemistry. (researchgate.net)
All types of nucleic acids from plasmid DNA or oligonucleotides to siRNA can be used with this approach [ 12 ]. (hindawi.com)
In the presence of nucleic acids and at low ionic strength ΔGag assembles into immature virus-like particles. (nih.gov)
Enzymes that catalyze the synthesis of FATTY ACIDS from acetyl-CoA and malonyl-CoA derivatives. (lookformedical.com)
A group of compounds that are derivatives of octadecanoic acid which is one of the most abundant fatty acids found in animal lipids . (lookformedical.com)
Recently, we have considered the use of DNAs and peptide nucleic acids for this purpose because oligomers can have hybridization affinities equivalent to that of biotin for streptavidin or avidin without the associated difficulties. (snmjournals.org)
Adenine (Ade) is a purine base, which is present in nucleic acids. (innovativechem.net)
Non-natural oligonucleotides are valuable probes of biological systems, as they can convey synthetic control of endogenous nucleic acids with high sequence specificity. (cdc.gov)
The WHO Monitoring Group on Gene transfer Medicinal Products was established to monitor devel- opments and draw up appropriate guidance for assuring the quality of gene transfer medicinal prod- ucts, including nucleic acids, viral and non-viral vectors, and genetically modified cells. (who.int)

Affinity chromatography is the preferred method of bioselective adsorption and subsequent recovery of a compound from an immobilized ligand. (sigmaaldrich.com)
Dye ligand chromatography is affinity chromatography that utilizes covalently bond textile dye (reactive dyes) to purify proteins. (sigmaaldrich.com)
31] AFFINITY CHROMATOGRAPHY 345 [31] Affinity Chromatography By PEDRO CUATRECASAS AND CHRISTIAN B. ANFINSEN General Considerations Conventional procedures of protein purification are generally based on relatively small differences in the physicochemical properties of the proteins in the mixture. (nih.gov)
The selective isolation and purification of enzymes and other biologically important macromolecules by "affinity chromatography" exploits the unique biological property of these pro- teins or polypeptides to bind ligands specifically and reversibly. (nih.gov)
Affinity chromatography may also be useful in concentrating dilute solutions of proteins, in removing denatured forms of a purified protein, and in the separation and resolution of protein components resulting from specific chemical modifications of purified proteins. (nih.gov)
The following general considerations should serve as guidelines in the preparation of adsorbents for affinity chromatography. (nih.gov)

Effect of metallic cations on human serum: Study by starch-gel electrophoresis. (cdc.gov)
In the process, we survey experimental techniques for determining protein abundance, principally two-dimensional gel electrophoresis and mass-spectrometry. (biomedcentral.com)
Determining relative protein expression levels by conventional two-dimensional electrophoresis requires isoelectric focusing, SDS-polyacrylamide gel electrophoresis, staining, fixing, densitometry, and careful matching of the same spots on two or more gels. (biomedcentral.com)
An attractive aspect of this approach is the low capital equipment cost, but a high level of expertise is needed to obtain reproducible gels, and two-dimensional electrophoresis is generally limited to proteins that are neither too acidic, too basic, nor too hydrophobic, and that are between 10 and 200 kDa in size, so that they are reliably separated on gels. (biomedcentral.com)
Two-dimensional fluorescence-difference gel electrophoresis (DIGE) utilizes mass- and charge-matched, spectrally resolvable fluorescent dyes (such as Cy3 and Cy5) to label two different protein samples in vitro prior to two-dimensional electrophoresis. (biomedcentral.com)
Its main advantage over conventional two-dimensional electrophoresis is that both the control and the experimental sample are run in a single polyacrylamide gel. (biomedcentral.com)
Proteomic analysis was also executed by using two dimensional gel electrophoresis (2DE) followed by liquid chromatography coupled to tandem mass spectrometry (LC-ESI/MS/MS). Real time PCR was used to confirm some of the genes. (biomedcentral.com)

A solution containing the macromolecule to be purified is passed through a column containing an insoluble polymer or gel to which a specific competitive inhibitor or other ligand has been covalently attached. (nih.gov)

The luminescent SYPRO protein gel stains are revolutionizing the detection of the total-protein profile in polyacrylamide gels. (thermofisher.com)

Phosphorodiamidate morpholino oligonucleotides are widely used to interrogate gene function in whole organisms, and light-activatable derivatives can reveal spatial and temporal differences in gene activity. (cdc.gov)

Many of the drawbacks of these traditional stains can be overcome by using one of the SYPRO protein gel stains, without sacrificing detection sensitivity. (thermofisher.com)

Moreover, silver staining shows very poor linearity with protein concentration ( Figure 9.3.1 ) and poor reproducibility in staining from gel to gel, making it inadequate for comparative studies of protein expression in cells. (thermofisher.com)
We have developed a SYPRO dye optimized for protein profiling in nearly every type of gel ( SYPRO and Coomassie Fluor luminescent and fluorescent protein gel stains-Table 9.2 ) or blot application ( Molecular Probes fluorescent and luminescent protein blot stains-Table 9.3 ). (thermofisher.com)
The characteristics and applications of the individual SYPRO protein gel and blot stains for detecting the total-protein profile of a sample are described in this section. (thermofisher.com)
The gels were stained with either SYPRO Ruby protein gel stain ( S12000 , S12001 , S21900 ) or a silver stain. (thermofisher.com)
SYPRO Ruby protein gel stain shows a linear quantitation range over three orders of magnitude, as well as consistent staining intensities from gel to gel. (thermofisher.com)

1984. Application of high-performance liquid chromatography with synchronized accumulating radioisotope detector to analysis of glyceryl trinitrate and its metabolites in rat plasma. (cdc.gov)
Comparative analysis of chemical constituents of Moringa oleifera leaves from China and India by ultra-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry. (rjptonline.org)

In contrast, the silver stain shows linear quantitation over only a small range, a very shallow slope and inconsistent staining intensities from gel to gel, even when corrected for background differences. (thermofisher.com)

Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix. (lookformedical.com)

Toxin B from strain 8864 and from VPI strain 10463, a strain that produces high levels of both toxin A and toxin B, was purified to homogeneity by sequential anion-exchange chromatography on DEAE-Sepharose CL-6B, gel filtration on Ultrogel AcA22, and immunoadsorption chromatography, and their toxic activities were compared. (nih.gov)
No stop solutions or destaining steps are required and, unlike silver staining, gels can be left in the dye solution for indefinite periods without overstaining, vastly simplifying the simultaneous processing of multiple gels and making it possible to perform high-throughput staining without investing in robotic staining devices. (thermofisher.com)

Agar has that unique property of holding so much water in its gels (99.9%) so it is used widely for such gelling and stabilizer qualities not only in food but also in other industries. (fao.org)

Harsh conditions may be avoided by using a biotin derivative such as 2-iminobiotin, which dissociates from avidin under milder conditions. (sigmaaldrich.com)

After fixation, the gel is incubated in the staining solution. (thermofisher.com)

Anatomy14

Organisms16

Chemicals and Drugs97

Analytical, Diagnostic and Therapeutic Techniques and Equipment73

Phenomena and Processes53

Disciplines and Occupations8

Technology, Industry, Agriculture2

Information Science4

Health Care5

Thin layer chromato2

Liquid chromatography2

Acid9

Proteins3

Biomolecules1

Amino acids1

Electrophoresis1

Synthesis2

Oxidize1

Filtration1

Synthetic3

Structures1

Aldehydes1

Extracellular2

Gene2

Sequence1

Widely1

Biological2

Genes3

Column2

Human1

Research2

Process2

Study1

Acids12

Affinity chromatography6

Electrophoresis7

Ligand1

Polyacrylamide1

Oligonucleotides1

Detection1

Protein5

Analysis2

Differences1

Types1

High2

Unique1

Conditions1

Solution1