• Nucleic Acids Res;2023 Oct 11. (bvsalud.org)
  • Nucleic Acids Res 2002, 30:e15. (lu.se)
  • Nucleic acid hybridization is at the core of many amplification-free molecular diagnostics and detection probe configuration is key to diagnostic performance. (psu.edu)
  • It notably includes recommendations for use of nucleic acid amplification tests, or NAATs, for Trichomonas vaginalis and routine trichomonas screening for high-risk populations. (genomeweb.com)
  • Herein, we propose a nucleic acid testing platform, named MiCaR, which couples a microfluidic device with CRISPR-Cas12a and multiplex recombinase polymerase amplification. (ufluidix.com)
  • Microfluidic space coding for multiplexed nucleic acid detection via CRISPR-Cas12a and recombinase polymerase amplification. (ufluidix.com)
  • Nucleic acid detection by isothermal amplification and the collateral cleavage of reporter molecules by CRISPR-associated enzymes is a promising alternative to quantitative PCR. (nature.com)
  • Primers for RT-LAMP or RT-RPA can be designed for specific viral RNA sequences, and the amplification methods are used before the Cas enzyme detection step, to multiply detection signals from the specific nucleic acid sequences. (nature.com)
  • Nucleic acid amplification (NAA) tests, such as polymerase chain reaction (PCR) and other methods for amplifying DNA and RNA, may facilitate rapid detection of microorganisms. (cdc.gov)
  • ABSTRACT: Nucleic acid sequence based amplification (NASBA) is an isothermal nucleic acid amplification procedure based on target-specific primers and probes, and the co-ordinated activity of 3 enzymes: AMV reverse transcriptase, RNase H, and T7 RNA polymerase. (int-res.com)
  • CDI cases are identified based on reports of positive C. difficile toxin assay or C. difficile nucleic acid amplification assay from all clinical, reference, and commercial laboratories that serve the population in the surveillance catchment areas. (cdc.gov)
  • Target amplification assays are based on the ability of complementary oligonucleotide primers to specifically anneal and allow enzymatic amplification of the target nucleic acid strands. (cdc.gov)
  • Detection of the rRNA amplification product sequences is achieved using nucleic acid hybridization (HPA). (cdc.gov)
  • The "gold standard" for COVID-19 detection is nucleic acid amplification for use of Ag-RDT testing (NAAT) where viral genomes are detected indicating the presence of the virus in an individual. (who.int)
  • Fast, inexpensive, and multiplexed detection of multiple nucleic acids is of great importance to human health, yet it still represents a significant challenge. (ufluidix.com)
  • The last 'wash' is often a dry step to allow the alcohol to evaporate, leaving only purified nucleic acids bound to the column. (wikipedia.org)
  • This document provides evidence-based clinical practice guidelines on the diagnostic utility of nucleic acid-based testing of respiratory samples for viral pathogens other than influenza in adults with suspected community-acquired pneumonia (CAP). (guidelinecentral.com)
  • Currently, there are three basic types of tests to determine if an individual has been infected with SARS-CoV-2: viral nucleic acid (RNA) detection, viral antigen detection, and detection of antibodies to the virus. (medscape.com)
  • Viral tests (nucleic acid or antigen detection tests) are used to assess acute infection, whereas antibody tests provide evidence of prior infection with SARS-CoV-2. (medscape.com)
  • Theoretically, nucleic acids-based antiviral therapeutics are dedicated to inhibiting the expression of viral or host factor genes via DNA or RNA. (creative-biolabs.com)
  • Le VRS demeure un agent étiologique viral important au Maroc, responsable de syndromes de type grippal et d'infections respiratoires aiguës sévères, en particulier chez les nourrissons. (who.int)
  • Viral nucleic acids are then extracted from the resin to facilitate molecular analyses through a reduction in the effective sample volume. (cdc.gov)
  • The laboratory diagnosis is usually performed by testing the plasma or serum to detect the virus, viral nucleic acid or virus specific immunoglobulin M and neutralizing antibodies. (bvsalud.org)
  • IIT Kharagpur has launched its flagship healthcare product, COVIRAP, a standard stepwise isothermal nucleic acid-based testing technology for the quick diagnosis of pathogenic infections including SARS-CoV-2 in individuals. (hospitalmanagement.net)
  • A method is described for visualizing three nucleic acid sequences simultaneously by in situ hybridization using a new blue immunofluorescent label, amino methyl coumarin acetic acid (AMCA), in combination with green and red fluorescing FITC and TRITC. (nih.gov)
  • Nucleic Acid-Based Identification Methods for Infectious Disease Nucleic acid-based methods detect organism-specific DNA or RNA sequences extracted from the microorganism. (msdmanuals.com)
  • Secondary structure can be predicted from one or several nucleic acid sequences. (wikipedia.org)
  • The new guidelines further mention two specific antigen detection and nucleic acid probe-hybridization tests. (genomeweb.com)
  • Modified nucleic acids such as peptide nucleic acid (PNA) offer advantages compared to conventional DNA probes allowing for faster hybridization, better stability and minimal sample preparation for direct detection of pathogens. (psu.edu)
  • Probes with tethered fluorophore and quencher allow for solution-based assays and eliminate the need for washing steps thereby facilitating integration into microfluidic devices. (psu.edu)
  • Beyond the bulk fluid assay and droplet devices, use of dsPNA probes may be advantageous in a wide variety of assays that employ homogenous nucleic acid hybridization. (psu.edu)
  • METHODS: RPCs of molecular weight (mwt) 45 and 187 kDa were prepared by oxidative polycondensation of the peptide Cys-Lys(10)-Cys and used to condense nucleic acids. (ox.ac.uk)
  • Nucleic acid-based (molecular) identification. (msdmanuals.com)
  • However, in the case of RNA much of the final structure is determined by the secondary structure or intra-molecular base pairing interactions of the molecule. (wikipedia.org)
  • DESTINA is a diagnostics company, offering innovative Nucleic Acid Testing and molecular diagnostics assays solutions, with a particular emphasis on RNA detection and quantification. (destinagenomics.com)
  • Gregory LA, Aguissa-Toure AH, Pinaud N, Legrand P, Gleizes PE, Fribourg S. Molecular basis of Diamond-Blackfan anemia: structure and function analysis of RPS19. (medlineplus.gov)
  • 2018 2 November 19/bio Valley BIOON/-in a new study, before the first time developed a rapid and inexpensive highly sensitive based on the CRISPR diagnostic tool-is called SHERLOCK(Specific High Sensitivity Enzymatic Reporter UnLOCKing the-the researchers greatly enhances this tool's capabilities, and developed a micro-strip test method, this test method allows the naked eye to the test results, without the use of expensive equipment. (crisprinsider.com)
  • papers communication the author, the Broadbent Institute core member Feng Zhang, Professor,"said SHERLOCK to provide an inexpensive, easy-to-use and sensitive diagnostic method to detect nucleic acid material-this can mean viruses, tumor DNA, and many other targets. (crisprinsider.com)
  • Therapeutic nucleic acids, also called therapeutic oligonucleotides or oligos, are short DNA and RNA molecules that serve as the API for drugs targeting a growing number of diseases, including cancer, cardiovascular disease, and rare and infectious diseases. (biospectrumasia.com)
  • The nitrogenous bases of nucleotides are organic (carbon-based) molecules made up of nitrogen-containing ring structures. (khanacademy.org)
  • Nucleic acid is constructed from simpler molecules called bases. (historyoftheuniverse.com)
  • If this water also contained sugars and other groups called phosphates then perhaps it was relatively easy for them to be dry-joined together to make the molecules we now call nucleic acids. (historyoftheuniverse.com)
  • Each base was attached to a sugar molecule, and the sugars were joined together by phosphate molecules to form a long chain. (historyoftheuniverse.com)
  • Nucleic acid aptamers are single-stranded oligonucleotides (RNA or DNA) 30 to 100 nt long that can bind to and affect the activity of target molecules with high specificity and affinity. (creative-biolabs.com)
  • many molecules have several possible three-dimensional structures, so predicting these structures remains out of reach unless obvious sequence and functional similarity to a known class of nucleic acid molecules, such as transfer RNA (tRNA) or microRNA (miRNA), is observed. (wikipedia.org)
  • Secondary structure of small RNA molecules is largely determined by strong, local interactions such as hydrogen bonds and base stacking . (wikipedia.org)
  • The stability of RPC-based polyplexes to reduction was determined using electrophoresis, dynamic light scattering and fluorescence techniques. (ox.ac.uk)
  • With only one fluorescence probe, MiCaR can simultaneously test up to 30 nucleic acid targets through microfluidic space coding. (ufluidix.com)
  • Evox Therapeutics is a privately held, Oxford-based biotechnology company focused on harnessing and engineering the natural delivery capabilities of extracellular vesicles, known as exosomes, to develop an entirely new class of therapeutics. (evoxtherapeutics.com)
  • Backed by leading life sciences venture capital groups and supported by a comprehensive intellectual property portfolio, Evox's mission is to positively impact human health by creating novel exosome-based therapeutics for the treatment of various severe diseases with limited options for patients and their families. (evoxtherapeutics.com)
  • Wooden shafts or calcium alginate swabs may contain substances that inhibit some viruses and nucleic acid detection reactions. (medscape.com)
  • Here, we adapted an anion exchange resin-based method, initially developed to concentrate negatively charged viruses from water, to liquid impingement-based bioaerosol sampling. (cdc.gov)
  • Her current work focuses on the synthesis of oligonucleotides and nucleic acid nanostructures with modified backbones to modulate their interactions with living systems. (mcmaster.ca)
  • She was previously a Banting and International Institute for Nanotechnology Postdoctoral Fellow in Prof. Chad Mirkin's group at Northwestern University where she developed an automated synthesis for oligonucleotides with guanidinium backbones and spherical nucleic acids with controlled surface charge. (mcmaster.ca)
  • Even prior to the nucleic acid methods employed today, it was known that in the presence of chaotropic agents, such as sodium iodide or sodium perchlorate, DNA binds to silica, glass particles or to unicellular algae called diatoms which shield their cell walls with silica. (wikipedia.org)
  • Using this artificial base, we have combined transient absorption and EPR spectroscopies as well as biochemical methods to test experimentally current mechanisms for DNA charge transport. (caltech.edu)
  • However, this method is limited because it, unlike some nucleic acid-based methods, is not readily deployable in the field. (msdmanuals.com)
  • For experimental methods, see Nucleic acid structure determination . (wikipedia.org)
  • Commonly used solvent-based and silica-based extraction methods leave behind chaotropic salts and other inhibitors that negatively affect results in downstream applications, such as qPCR. (the-scientist.com)
  • Nucleic acid extraction from plants by negative chromatography provides better yield than traditional silica methods and reduces impurities, providing superior performance in downstream analyses. (the-scientist.com)
  • A common problem for researchers working with RNA is to determine the three-dimensional structure of the molecule given only a nucleic acid sequence. (wikipedia.org)
  • Xanthine is not part of the genetic code, but it is found in living organisms from the breakdown of guanine, which is a base in DNA and RNA. (creation.com)
  • the data also indicate no evidence of guanine radicals, given the low oxidation potential of 4-methylindole relative to the nucleic acid bases. (caltech.edu)
  • Bases include the pyrimidine bases (cytosine, thymine in DNA, and uracil in RNA, one ring) and the purine bases (adenine and guanine, two rings). (khanacademy.org)
  • Each nucleotide in DNA contains one of four possible nitrogenous bases: adenine (A), guanine (G) cytosine (C), and thymine (T). Adenine and guanine are purines , meaning that their structures contain two fused carbon-nitrogen rings. (khanacademy.org)
  • Life mostly uses four different types of base: thymine, cytosine, guanine and adenine (sometimes shortened to TCGA). (historyoftheuniverse.com)
  • Guanine quadruplexes are nucleic acid structures built ions in slow exchange with bulk ions. (lu.se)
  • With the rapid advancements in AI-based time trajectory analysis and cell subpopulation identification, there exists a pressing need for a database that not only provides SCP raw data but also explicitly describes experimental details and protein expression profiles. (bvsalud.org)
  • It was unique in (a) systematically providing the SCP raw data for both mass spectrometry -based and flow cytometry -based studies and (b) explicitly describing experimental detail for SCP study and expression profile of any studied protein . (bvsalud.org)
  • Areas covered: Here we review the use of attenuated protein toxins as a delivery vehicle for nucleic acids, their relationship to the PEG dilemma, and their biological properties with specific reference to their intracellular trafficking. (figshare.com)
  • Exosome-based drugs have the potential to address some of the limitations of protein, antibody and nucleic acid-based therapies by enabling delivery to cells and tissues that are currently out of reach using other drug delivery technologies, and Evox is leading the development within this emerging therapeutic space. (evoxtherapeutics.com)
  • This article reports on the characterisation of a new type of synthetic vector based on a linear reducible polycation (RPC) that can be cleaved by the intracellular environment to facilitate release of nucleic acids. (ox.ac.uk)
  • Liu, W T. / Nucleic-acid-based characterisation of community structure and function . (elsevierpure.com)
  • Nucleic acid isolation from plant tissues presents unique challenges. (the-scientist.com)
  • The use of palladium-based labeling reagents expands the number of measurement channels available for mass cytometry and reduces interference with lanthanide-based antibody measurement. (nature.com)
  • Spin column-based nucleic acid purification is a solid phase extraction method to quickly purify nucleic acids. (wikipedia.org)
  • PMID 6179438 Boom R, Sol CJ, Salimans MM, Jansen CL, Wertheim-van Dillen PM, van der Noordaa J. Rapid and simple method for purification of nucleic acids. (wikipedia.org)
  • In this Technique Talk, April Bauer will discuss negative chromatography-based purification for plant nucleic acids using GenElute™-E purification kits as well as sample preparation and PCR reagent selection for successful qPCR results. (the-scientist.com)
  • An engineered CRISPR-based method that finds RNA from SARS-CoV-2, the virus that causes COVID-19, promises to make testing for that and other diseases fast and easy. (technologynetworks.com)
  • In this proof-of-concept study, we report a PCR-free and label-free detection method that has a limit of detection (3 standard deviations) of 15 fmoles of microRNA122, by integrating a dynamic chemical approach for "Single Nucleobase Labelling" with a bead-based platform (Luminex ® ) thereby, in principle, demonstrating the exciting prospect of rapid and accurate profiling of any microRNAs related to diseases and toxicology. (destinagenomics.com)
  • The basis of the stacking interactions lies in the pi-electron structure of individual nucleobases, which can be described by various aromaticity indices. (udg.edu)
  • In particular, the bases found were uracil , a pyrimidine (single-ringed base), and xanthine , a purine (double-ringed base). (creation.com)
  • Drug resistance val (CI) = 1.4-62) did not outperform corresponding classifiers based on clinical variables. (lu.se)
  • Our data have provided evidence for a comparable prediction of clinical outcome in CMF-treated breast cancer patients using conventional clinical variables and gene expression based markers. (lu.se)
  • Based on the typical clinical features, differential diagnosis for infection by Zika virus is widespread. (bvsalud.org)
  • The preliminary diagnosis is based on clinical features, places and dates of the patient and their activities. (bvsalud.org)
  • This video will show this procedure to remove nucleic acid from a specimen. (cdc.gov)
  • Based on the detection of cell culture-derived nodavirus, and a synthetic RNA target, the real-time NASBA procedure was approximately 100-fold more sensitive than single-tube RT-PCR. (int-res.com)
  • Perspectives on analyses of nucleic acid constituents: The basis of ge" by Phyllis R. Brown, Christina S. Robb et al. (uri.edu)
  • As to polymer devices, a citrate-based fibre with improved optical and mechanical properties was developed and successfully tested on imaging and in vivo analyses 3 . (nature.com)
  • Each nucleotide is made up of three parts: a nitrogen-containing ring structure called a nitrogenous base, a five-carbon sugar, and at least one phosphate group. (khanacademy.org)
  • The sugar molecule has a central position in the nucleotide, with the base attached to one of its carbons and the phosphate group (or groups) attached to another. (khanacademy.org)
  • Image of the components of DNA and RNA, including the sugar (deoxyribose or ribose), phosphate group, and nitrogenous base. (khanacademy.org)
  • Column-based extraction is a method that employs selective binding of nucleic acid to a solid matrix, such as silica that is packed in a column. (cdc.gov)
  • Nucleic acid-based diagnostics. (who.int)
  • This method relies on the fact that nucleic acid will bind to the solid phase of silica under certain conditions. (wikipedia.org)
  • Nucleic acid structure prediction is a computational method to determine secondary and tertiary nucleic acid structure from its sequence. (wikipedia.org)
  • This article is about the computational prediction of nucleic acid structure. (wikipedia.org)
  • However, DNA-based transporters are limited by their low cellular uptake and show rapid degradation in serum. (mcmaster.ca)
  • The centrifuge/vacuum forces the solution through a silica membrane that is inside the spin column, where under the right ionic conditions, nucleic acids will bind to the silica membrane, as the rest of the solution passes through. (wikipedia.org)
  • Several other NAA tests are under commercial development, including the Roche AmplicorTM test (4), a PCR-based test that amplifies mycobacterial DNA. (cdc.gov)
  • NMR spectroscopy or X-ray crystallography, but important width measurements at a single resonance frequency (or questions remain concerning the energetics, stability, and magnetic field strength), making it difficult to separate dynamics of this structurally diverse nucleic acid class. (lu.se)
  • Agilent Technologies Inc. has announced that it is investing approximately $725 million to double manufacturing capacity of therapeutic nucleic acids in response to rapid growth of the $1 billion market and strong demand for the company's high-quality active pharmaceutical ingredients (API). (biospectrumasia.com)
  • The funds will be used to support the Company's development of cell-targeted exosome-based approaches to more effectively deliver a nucleic acid-based anti-infective drug. (evoxtherapeutics.com)
  • [7] [8] One of the early attempts at predicting RNA secondary structure was made by Ruth Nussinov and co-workers who developed a dynamic programming-based algorithm that maximized the length and number of a series of "blocks" (polynucleotide chains). (wikipedia.org)