• Comparative miRNA array led to the isolation of several Bone Morphogenic Protein 2 (BMP2)-responsive miRNAs. (wikipedia.org)
  • Still other RNA molecules, such as tiny microRNAs ( miRNAs ), act as regulators of other genes, and new types of non-protein-coding RNAs are being discovered all the time. (khanacademy.org)
  • MicroRNAs (miRNAs) are small non-coding nucleic acids that can regulate post-transcriptional gene expression by binding to complementary sequences of target mRNA. (birmingham.ac.uk)
  • In this study, we combined the results of two independent systematic reviews aiming to unveil the co-expression network of miRNAs and proteins in brain tissues of AD patients. (birmingham.ac.uk)
  • Twenty-eight studies including a total of 113 differentially expressed miRNAs (53 of them validated by qRT-PCR), and 26 studies including a total of 196 proteins differentially expressed in AD brains compared to healthy age matched controls were selected. (birmingham.ac.uk)
  • MicroRNAs (miRNAs) are short non-coding RNAs that posttranscriptionally regulate gene expression inside the cell. (frontiersin.org)
  • This review focuses on the mechanisms by which vesicle-free miRNAs are secreted from neuroendocrine cells and will discuss potential functions of vesicle-free miRNAs and how vesicle-free miRNAs regulate cell-to-cell communication. (frontiersin.org)
  • 60% of human protein-coding genes, mostly by binding to the 3′- untranslated region (3′UTR) of the target mRNAs ( 5 ) and, therefore, miRNAs affect gene expression networks of a variety of biological processes including development, apoptosis, proliferation, and metabolism ( 1 , 2 ). (frontiersin.org)
  • More than 90% of extracellular miRNAs are vesicle-free, but form a complex with proteins such as Agonaute2 (AGO2) ( 22 , 23 ). (frontiersin.org)
  • RNA polymerase II mainly transcribes microRNA genes as primary miRNA transcripts (pri-miRNAs) that contain 5′cap and 3′poly(A) tails ( 28 ). (frontiersin.org)
  • Drosha, RNase III, and DGCR8, the RNA-binding protein, further process pri-miRNAs into stem-loop structured precursor miRNAs (pre-miRNAs) of ~70 nt ( 29 ). (frontiersin.org)
  • After pre-miRNAs are transported to the cytoplasm, RNase III Dicer and TRBP (transactivation-response RNA-binding protein) cleave them into double-stranded miRNA duplexes of ~22 nt ( 30 , 31 ). (frontiersin.org)
  • miRNAs comprise a large group of endogenous non-coding RNAs that can block mRNA translation or negatively regulate mRNA stability and thereby play a central role in regulating gene expression [10] , [11] . (plos.org)
  • A large number of studies have demonstrated that the synergistic collaboration of a number of microRNAs (miRNAs), their growth factors and their downstream agents is required for the initiation and completion of pathogenesis in the liver. (wjgnet.com)
  • Furthermore, we discuss recent findings on EBV associated gastric carcinogenesis by focusing on the roles of latent genes, epigenetic abnormalities, genomic alterations, and post-transcriptional regulation by cellular and viral microRNAs (miRNAs). (spandidos-publications.com)
  • Non-coding RNAs (ncRNAs), mainly including microRNAs (miRNAs), long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs), can function as important regulators in gene expression. (biomedcentral.com)
  • Detecting microRNA (miRNA) using nanopore single molecule detector Gu, Li-Qun MicroRNAs (miRNAs) are a class of short (~18-24-nt) non-coding RNAs molecules that regulate gene expression at the post- transcriptional level. (umsystem.edu)
  • MicroRNAs (miRNAs) are short non-coding RNAs, which are shown to post-transcriptionally regulate gene expression. (qiagen.com)
  • that asthma is intensively regulated by a variety of microRNAs (miRNAs). (cdc.gov)
  • MiRNAs are approximately 19- to 25-nucleotide estimated about 250,000 and mortality does not appear single-stranded, noncoding RNAs that exist in both to correlate well with prevalence. (cdc.gov)
  • As binding of miRNAs to a target mRNA may result in an airway inflammatory disease closely correlates with either mRNA degradation or inhibition of protein immune regulation, more and more evidences suggest translation.1 More than 2000 miRNAs have been discovered in humans. (cdc.gov)
  • Th)1 cells.5 Meanwhile, the function and immunity of with genetic predisposition, involving multiple cells, DCs are closely regulated by miRNAs. (cdc.gov)
  • An interaction network formed by DE mRNAs, DE miRNAs, and important pathways was discovered after we analyzed the functional enrichment, protein-protein interactions, and miRNA targetome prediction analysis. (frontiersin.org)
  • DEFB1 expression after transfection with two micro RNAs (miRNAs), hsa-miR-186-5p and hsa-miR-340-5p, provided evidence that DEFB1 expression could be modulated by these miRNAs and hsa-miR-186-5p had a higher binding efficiency with DEFB1. (cdc.gov)
  • Simple Summary: This study identified a set of 73 microRNAs (miRNAs) that can accurately detect lung cancer tumors from normal lung tissues. (cdc.gov)
  • This study identified a set of 73 microRNAs (miRNAs) that classified lung cancer tumors from normal lung tissues with an overall accuracy of 96.3% in the training patient cohort (n = 109) and 91.7% in unsupervised classification and 92.3% in supervised classification in the validation set (n = 375). (cdc.gov)
  • Experimentally confirmed target genes were identified for the 73 diagnostic miRNAs, from which proliferation genes were selected from CRISPR-Cas9/RNA interference (RNAi) screening assays. (cdc.gov)
  • MicroRNAs (miRNAs) are an integral part of the post-transcriptional machinery of gene expression and have been implicated in the carcinogenic cascade. (cdc.gov)
  • Previous findings indicate that circRNAs mainly function as microRNA (miRNA) sponges to regulate the transcription and posttranscription of miRNA-targeted genes. (hindawi.com)
  • A further bioinformatic analysis was performed to match miRNA and protein targets with an inverse relation. (birmingham.ac.uk)
  • in this way, vesicle-free miRNA may regulate cell-to-cell communication including the regulation of gene expression and cellular signaling. (frontiersin.org)
  • We previously showed that VEGF signaling in primary endothelial cell cultures caused overexpression of VEGFR2 in a positive feed-forward loop, which is at least partly regulated by loss of miRNA-mediated control of VEGF receptor degradation. (plos.org)
  • Furthermore, pumilio homolog 2 (PUM2), a RNA-binding protein, mediated the packaging of miRNA-130a into exosomes. (dovepress.com)
  • Potential Areas of Applications: Detection of any nucleic acids fragments, including all microRNAs and pathogenic DNAs or RNAs, Study of nucleic acid folding/unfolding reactions, Study of miRNA mechanism and principle of regulation Study single nucleotide polymorphisms (SNPs), Study of oligonucleotide-protein and oligonucleotide-drug interactions for drug discovery and development, Biomarker characterization, Diagnostics, prognostics and theranostics. (umsystem.edu)
  • An emerging therapeutic target is microRNA (miRNA), small noncoding RNAs that negatively regulate sets of proteins. (futureneurocentre.ie)
  • We review design considerations for nucleic acid-based approaches and the challenges and next steps in developing therapeutic miRNA-targeting molecules for epilepsy. (futureneurocentre.ie)
  • The invention comprises "a method of diagnosing, assessing susceptibility, and/or treating schizophrenia involving the identification and/or observation of microRNAs and variant miRNA," according to the patent's abstract. (genomeweb.com)
  • microRNA alleles associated with schizophrenia and schizophrenia spectrum disorders were identified and ultra-rare variants in the precursor or mature miRNA were identified. (genomeweb.com)
  • However, robust quantification of miRNA and CNV can be difficult, especially in samples with a high inhibitory burden or low nucleic acid content. (qiagen.com)
  • microRNA (miRNA) is considered as one of these mediators. (frontiersin.org)
  • NEAT1 scaffolds RNA-binding proteins and the Microprocessor to globally enhance pri-miRNA processing. (ablife.cc)
  • Based on our identified miRNA-regulated network, we discovered three drugsBX-912, daunorubicin, and midostaurinthat can be repositioned to treat lung cancer, which was not known before. (cdc.gov)
  • Based on our identified miRNA-regulated molecular machinery, an inhibitor of PDK1/Akt BX-912, an anthracycline antibiotic daunorubicin, and a multi-targeted protein kinase inhibitor midostaurin were discovered as potential repositioning drugs for treating lung cancer. (cdc.gov)
  • Many genes encode protein products, meaning that they specify the sequence of amino acids used to build a particular protein. (khanacademy.org)
  • Importantly, not all genes encode protein products. (khanacademy.org)
  • For instance, some genes specify ribosomal RNAs ( rRNAs ), which serve as structural components of ribosomes, or transfer RNAs ( tRNAs ), cloverleaf-shaped RNA molecules that bring amino acids to the ribosome for protein synthesis. (khanacademy.org)
  • The AU-rich element RNA-binding protein 1 (AUF1) is an RNA-binding protein, which can both stabilize and destabilize the transcripts of several cancer-related genes. (nature.com)
  • A growing body of evidence shows that lncRNAs play important functional roles in diverse biological processes, such as epigenetic regulation, cell cycle regulation, cellular growth and differentiation, by regulating the level of target genes [ 3 , 4 ]. (biomedcentral.com)
  • They bind to messenger RNAs expressed by genes and break them down or prevent them from being translated into proteins. (fapesp.br)
  • After the characterization stage, we had to find out which cellular migration and invasion genes were regulated by miR-450a," Silva Junior said. (fapesp.br)
  • The researchers found that miR-450a blocked genes associated with vimentin, a protein in the cell invasion pathway. (fapesp.br)
  • Herein, to provide better understanding of the molecular events in GBC pathogenesis, we developed GBCdb ( http://tmliang.cn/gbc/ ), a user-friendly interface for the query and browsing of GBC-associated genes and RNA interaction networks using published multi-omics data, which also included experimentally supported data from different molecular levels. (biomedcentral.com)
  • Different innate immune pathways converge to Stimulator of interferon genes (STING) and trigger type I interferon responses after recognition of abnormal nucleic acids in the cells. (bvsalud.org)
  • In higher eukaryotes, protein-coding genes create circRNAs through exon back-splicing. (bvsalud.org)
  • We previously identified differentially expressed microRNAs with liver-specific target genes in plasma from children with chronic hepatitis B. To further understand the biological role of these microRNAs in the pathogenesis of chronic hepatitis B, we have used the human liver cell line HepG2, with and without HBV replication, after transfection of hepatitis B virus expression vectors. (biomedcentral.com)
  • RT-qPCR is the preferred method for microRNA studies, and a careful normalisation strategy, verifying the optimal set of reference genes, is decisive for correctly evaluating microRNA expression levels. (biomedcentral.com)
  • A panel of 739 microRNAs was screened to identify the most stably expressed microRNAs, followed by a PubMed search identifying microRNAs previously used as reference genes. (biomedcentral.com)
  • We identified miR-24-3p, miR-151a-5p, and miR-425-5p as the most valid combination of reference genes for microRNA RT-qPCR studies in our hepatitis B virus replicating HepG2 cell model. (biomedcentral.com)
  • Emerging evidences suggest that microRNA target genes that regulate DNA replication and cell cycle progression and we aim to determine how microRNA regulate the DNA replication machinery as cell progresses from one phase to the next. (nii.res.in)
  • Pansensitive and panresistant genes to 21 NCCN-recommended drugs with concordant mRNA and protein expression were identified. (cdc.gov)
  • MicroRNAs are regulatory molecules and suggested as non-invasive biomarkers for molecular diagnostics and prognostics. (biomedcentral.com)
  • Such methods generally comprise providing a cell with one or more precursor nucleic acid sequences that encode two or more RNA molecules. (genomeweb.com)
  • The well-known function of AUF1 is the destabilization of mRNAs by forming complexes with other proteins. (nature.com)
  • EIF5B (Eukaryotic Translation Initiation Factor 5B) is a conserved eukaryotic translation factor that mediates association of the 40S and 60S ribosomal subunits during eukaryotic translation initiation, and modulates the cell cycle progression by regulating the translation of upstream open reading frames (uORF)-containing mRNAs such as p27 and p21 [ 3 ]. (techscience.com)
  • In the glioma cells, EIF5B promotes cell survival by enhancing the translation of several IRES-containing mRNAs including those encoding anti-apoptotic proteins such as XIAP and Bcl-xL [ 4 ]. (techscience.com)
  • For RNA, hundreds of RNA binding proteins (RBPs) have now been identified which interact with mRNAs, noncoding RNAs, microRNAs etc. and are involved in post-transcriptional gene regulation including gene silencing by RNAi. (cambridgeproteinarrays.com)
  • In the analysis we compared and noted all the changes of the amount as well as type of mRNAs found in each stages of brain development and use them to infer the underlying communicational networks at the protein level. (grmrc.org)
  • We identified 70 mRNAs/proteins and groups of novel RNAs that do not bear any information for the production of proteins. (grmrc.org)
  • These mRNAs or novel RNAs were specifically produced in specific regions of the brain at specific developmental stages. (grmrc.org)
  • Although more and animals and plants and regulate gene/protein expression more progressions have been made about the through direct complementarity between their 5' region pathogenesis of asthma in recent years, the increasing and the 3' untranslated region of target mRNAs. (cdc.gov)
  • Many ncRNAs have critical roles in tumorigenesis, and some ncRNAs function as competing endogenous RNAs (ceRNAs) to perturb gene expression. (biomedcentral.com)
  • The purpose of this study was to elucidate the regulation of programmed death ligand 1 (PDL1), lactate dehydrogenase A (LDHA) and miR-34a in triple negative breast cancer (TNBC) and to explore the function and mechanism of PDL1 and LDHA as competitive endogenous RNAs (ceRNAs) in TNBC via regulation of miR-34a. (biomedcentral.com)
  • It is reported that RNAs can act as competitive endogenous RNAs (ceRNAs) to co-regulate each other by competing for shared microRNAs [ 8 , 9 ], and the 3′ UTR of mRNA alone is capable of eliciting ceRNA effects [ 10 ]. (biomedcentral.com)
  • however, the down-regulated endogenous miR-186 can cause the improved BACE1 level (23). (bibf1120.com)
  • Similarly, the expression of miR-512 from Tau protein-rich brains of the patients with advanced AD is significantly reduced, indicating that miR-512 can negatively regulate Tau protein through targeting Fas-related death domain protein (32). (bibf1120.com)
  • Nucleic acids, and DNA in particular, are key macromolecules for the continuity of life. (khanacademy.org)
  • Nucleic acids are the most important macromolecules for the continuity of life. (libretexts.org)
  • The method for studying protein - nucleic acid interactions on HuProt arrays resembles that for protein-protein interactions and can lead to identification of novel binding proteins, complementing other physical and genetic methods and contributing to understanding of the functional pathways of nucleic acid interactomes. (cambridgeproteinarrays.com)
  • A single microRNA can have multiple downstream targets and affect a number of different networks and pathways [ 4 , 5 ]. (biomedcentral.com)
  • In addition, Ingenuity Pathway Analysis (IPA) integrated with PCR array data showed that the JAK1/STAT3 pathway was significantly altered in cells overexpressing DEFB1, suggesting this to be one of the pathways by which defensin regulates IAV replication in HBEpCs. (cdc.gov)
  • to other RNAs may initiate epige- cleus pathways. (who.int)
  • Regulatory non-coding RNAs and RNA-binding proteins (RBPs) are important research areas in gene regulation and RNA biology. (rochester.edu)
  • Our laboratory is interested in the understanding of pathophysiological function and molecular mechanism of new non-coding RNAs (and RBPs) and new modes of gene regulation in c. (rochester.edu)
  • In this review, the role of microRNAs as biomarkers used in the diagnosis of AMI and HF is discussed, their advantage over traditional biomarkers is outlined and the potential for their implementation in clinical practice is critically assessed. (lancs.ac.uk)
  • Instead we use an in vitro HBV-replicating liver cell model to investigate the biological role of microRNAs with a possible influence on the pathogenesis of CHB in children. (biomedcentral.com)
  • MicroRNAs like miR-450a are small RNAs that do not encode proteins but perform a regulatory role in the genome and hence in several intracellular processes. (fapesp.br)
  • Protein arginine methyltransferase (PRMT)-mediated arginine methylation is an important post-transcriptional modification that regulates various cellular processes including epigenetic gene regulation, genome stability maintenance, RNA metabolism, and stress-responsive signal transduction. (bvsalud.org)
  • Targeted Transcriptomics of Frog Virus 3 in Infected Frog Tissues Reveal Non-Coding Regulatory Elements and microRNAs in the Ranaviral Genome and Their Potential Interaction with Host Immune Response. (xenbase.org)
  • Human genome codes for many other single strand DNA binding proteins and we are testing if novel mechanisms of checkpoint activation exist that do not require Replication Protein A. If such mechanisms exist we would like to understand which factors provide the role of DNA binding, how checkpoint proteins like Chk1 and p53 are activated and how signal transduction pathway stalls the cell cycle and DNA replication. (nii.res.in)
  • ing of microRNAs to the genome or inside the cel via membrane-to-nu- 2011 ). (who.int)
  • Long noncoding RNAs (lncRNAs) have recently been reported to be key regulators in the responses of plants to stress conditions, but the mechanism through which LP stress mediates the biogenesis of lncRNAs in soybean remains unclear. (biomedcentral.com)
  • PRMTs have been found to methylate and regulate several host cell proteins and different functional types of viral proteins, such as viral capsids, mRNA exporters, transcription factors, and latency regulators. (bvsalud.org)
  • TIME-related RNA or RNA regulators could be promising targets for anticancer immunotherapy. (biomedcentral.com)
  • Structures of mammalian GLD-2 proteins reveal molecular basis of their functional diversity in mRNA and microRNA processing. (xenbase.org)
  • My research group is interested in deciphering the genetic components that regulate the molecular networks underlying the development of the mammalian brain. (grmrc.org)
  • Summing up, we are trying to understand the mechanisms by which microRNAs regulate mammalian cell cycle and DNA replication in normal and pathological conditions. (nii.res.in)
  • GBCdb will help to elucidate the potential biological roles of different RNAs and allow for the exploration of RNA interactions in GBC. (biomedcentral.com)
  • This modulation affects their activity, subcellular localization, protein-nucleic acid and protein-protein interactions, ultimately impacting their roles in various virus-associated processes. (bvsalud.org)
  • In this review, we explore the pivotal roles of viral circRNAs and associated RNA in various biological processes. (bvsalud.org)
  • MicroRNAs participate in the regulation of asthma, the goal of this study is to summarize recent researches on the roles of microRNAs in the pathogenesis of asthma. (cdc.gov)
  • The results of the present study indicate that microRNAs play important roles in regulating asthma immune responses. (cdc.gov)
  • The miR-199 microRNA precursor is a short non-coding RNA gene involved in gene regulation. (wikipedia.org)
  • Circular RNA (circRNA) forms closed loops via back-splicing in precursor mRNA, resisting exonuclease degradation. (bvsalud.org)
  • nc886 is a 101 nucleotide long non-coding RNA that has been designated as a precursor microRNA or a vault RNA based upon it sequence. (oncotarget.com)
  • Pro- and anti-apoptotic microRNAs are differentially regulated during estivation in Xenopus laevis. (xenbase.org)
  • Moreover, miR-15a can target extracellular Rabbit Polyclonal to MC5R signal-regulated kinase BMS-790052 kinase activity assay 1 (ERK1) for the involvement of Tau hyperphosphorylation (9). (bibf1120.com)
  • The expression levels of RNA of hsa_circ_0046060, mmu_circ_0002819, and miR-338-3p were determined by quantitative real-time polymerase chain reaction (RT-qPCR). (hindawi.com)
  • In eukaryotes, most lncRNAs are transcribed by RNA polymerase II and have a structure similar to that of mRNA, which includes 5′ capping, splicing and polyadenylation [ 2 ]. (biomedcentral.com)
  • I propose to decipher the basic rules that govern the readout of the CTD code of RNA polymerase II by RNA processing factors. (muni.cz)
  • The concept of the CTD code that specifies the position of RNA polymerase II in the transcriptional cycle and thus recruits specific processing factors, was suggested almost a decade ago. (muni.cz)
  • However, how the C-terminal domain (CTD) of RNA polymerase II recruits, activates, and displaces appropriate processing factors in coordination with the transcription cycle, remains obscure. (muni.cz)
  • In the past few years circulating nucleic acids, notably microRNA that are small non-coding RNAs that regulate various cellular processes, have been investigated as biomarkers of disease offering improved sensitivity and specificity in the diagnosis and prognostication of various conditions. (lancs.ac.uk)
  • This process is regulated by both viral and cellular factors, which are necessary for an efficient viral replication as well as for the setting up of viral latency, leading to a repressed transcription of the integrated provirus.nnRESULTS: In this study, we examined the role of two parameters in HIV-1 LTR promoter activity. (cnrs.fr)
  • microRNAs are transcribed as ~70 nucleotide precursors and subsequently processed by the Dicer enzyme to give a ~22 nucleotide product. (wikipedia.org)
  • Before this information can be used for protein synthesis, however, an RNA copy (transcript) of the gene must first be made. (khanacademy.org)
  • A complex metabolic network consisting of enzymes catalyzing their synthesis, modification (phosphorylation, glycosylation) and breakdown regulates accumulation of sphingolipid species and thereby the sphingolipid pool at rheostat conditions, and this can undergo substantial changes in response to metabolic and external challenges. (encyclopedia.pub)
  • Whenever genomic lesions are detected during DNA synthesis, they are coated by the single strand DNA binding protein complex, called Replication Protein A, leading to the activation of canonical checkpoint proteins like ATR, Chk1 and p53. (nii.res.in)
  • Rat hippocampi were micro-dissected (for mRNA, microRNA, and DNA methylation analysis) or frozen whole (for chromatin immunoprecipitation). (karger.com)
  • In eukaryotic cells but not in prokaryotes, DNA forms a complex with histone proteins to form chromatin, the substance of eukaryotic chromosomes. (libretexts.org)
  • DNA-binding domains, zinc fingers, leucine zippers) and histones and nonhistone proteins interacting with chromatin, all of which can influence gene expression. (cambridgeproteinarrays.com)
  • Molecular Therapy-Nucleic Acids, 2023. (jhhlab.tw)
  • Some viruses use RNA, not DNA, as their genetic material, but aren't technically considered to be alive (since they cannot reproduce without help from a host). (khanacademy.org)
  • Furthermore, since a number of genetic disorders or human diseases (including cancer or neurodegenerative diseases) relate to abnormalities in RNA processing and proper ribonucleoprotein assembly, we anticipate that the detailed knowledge of mechanisms regulating the co-transcriptional processing will be of clinical value in the long term. (muni.cz)
  • Even small changes in microRNA expression may therefore have implications for gene regulation in various physiological and pathophysiological states [ 1 , 6 ]. (biomedcentral.com)
  • It has been observed that some replication proteins localize to the centrosome suggesting their role in regulation of centrosome biogenesis. (nii.res.in)
  • To elucidate the regulation of PDL1, LDHA and miR-34a in TNBC, this study will explore the function of miR-34a as a tumor suppressor that regulates glycolysis and immunology in TNBC cells by targeting LDHA and PDL1 and examine the mechanism of PDL1 and LDHA as ceRNAs that promote proliferation and metastasis of TNBC by regulating miR-34a. (biomedcentral.com)
  • Her Ph.D. research focused on the transcriptional regulation of the unfolded protein response in both fungi and higher eukaryotes. (qiagen.com)
  • MicroRNAs not only participate in determining DCs phenotype and then naive T lymphocyte differentiation, but also participate in the regulation of airway inflammation and airway remodeling in asthma. (cdc.gov)
  • Zhang et al cytokines, mediators and signals and closely related to demonstrated that microRNA let-7i was upregulated immune regulation. (cdc.gov)
  • Recent advances in biological technologies, such as tiling arrays and RNA deep sequencing (RNA-seq), have made it possible to survey the transcriptomes of many organisms to an unprecedented degree [ 6 ]. (biomedcentral.com)
  • This progression from DNA to RNA to protein is called the " central dogma " of molecular biology. (khanacademy.org)
  • Exosomes secreted by the umbilical cord mesenchymal stromal cells (UMSCs) and circular RNAs (circRNAs) derived from exosomes have been shown to be associated with the progression of GDM-related complications. (hindawi.com)
  • Depending on the length of their fatty acid chains and their degree of saturation, SL species have a strong impact on biophysical membrane parameters such as fluidity or rigidity and curvature, and on interaction with membrane proteins and/or cytoskeletal components, and membrane compartmentalization. (encyclopedia.pub)
  • Similar to array-based protein-protein interaction screening, the purified nucleic acid of interest is either fluorescently labelled for direct detection on the array or alternatively, a biotinylated nucleic acid can be detected by secondary incubation with fluorophore-labelled streptavidin. (cambridgeproteinarrays.com)
  • 98% of RNA transcripts in humans are non-coding ( 3 ). (frontiersin.org)
  • Our group are interested in deciphering the role of these differentially regulated transcripts in brain development. (grmrc.org)
  • The microRNA-Medicated Hyperphosphorylation of Tau Proteins in Advertisement And a, the build up of intracellular insoluble hyperphosphorylated Tau proteins can be another pathological feature in Advertisement. (bibf1120.com)
  • While no inverse relationships between miR-132-3p and proteins were found, miR-16′s inverse relationship with CLOCK proteins in the circadian rhythm pathway is discussed and therapeutic targets are proposed. (birmingham.ac.uk)
  • With the technology available at the NIH lab to look for noncoding RNA targets, we discovered that this microRNA also caused cell death by reducing cell energy. (fapesp.br)
  • MicroRNAs are suggested as non-invasive biomarkers for molecular diagnostics and prognostics, and some microRNAs show promising results as therapeutic targets in human trials [ 1 - 3 ]. (biomedcentral.com)
  • Furthermore, microRNAs are also shown to be targets for asthma therapy in the future. (cdc.gov)
  • This combined approach will provide us with the entirely new structural information that will go significantly beyond the-state-of-the-art in the research fields of transcription and RNA processing. (muni.cz)
  • DNA-protein interactions occur via a range of binding motifs present in transcription factors and nuclear receptors (e.g. (cambridgeproteinarrays.com)
  • Bases include the pyrimidine bases (cytosine, thymine in DNA, and uracil in RNA, one ring) and the purine bases (adenine and guanine, two rings). (khanacademy.org)
  • More importantly, we summarize the potential of various RNA-based therapeutics clinically available for cancer treatment. (biomedcentral.com)
  • RNA-dependent targeting of the TIME, as monotherapy or combined with other evolving therapeutics, might be beneficial for cancer patients' treatment in the near future. (biomedcentral.com)
  • Cancer-associated fibroblasts (CAFs) have been demonstrated to be essential stromal components in the tumor microenvironment, regulating cancer cell growth, migration, invasion, and chemo-response through various mechanisms. (dovepress.com)
  • Profiling of nucleic acid binding on HuProt human proteome microarrays can reveal interactions with the largest collection of individual human proteins available on a single slide. (cambridgeproteinarrays.com)
  • He received his Ph.D. degree in the laboratory of Dr. En-Duo Wang in Biochemistry and Molecular Biology from Shanghai Institute of Biochemistry and Cell Biology of Chinese Academy of Science in 2008, where he studied the structural and functional interplay of transfer RNA (tRNA) and tRNA synthetase in bacteria, yeast and human. (rochester.edu)
  • We recently discovered a new type of stress-responsive, protein-directed human RNA switch that regulates expression of vascular endothelial growth factor-A in human monocytic cells (Ray, PS, et al. (rochester.edu)
  • Studies report that circular RNAs (circRNAs) derived from exosomes may play a key role in GDM development. (hindawi.com)
  • CircRNAs are newly discovered noncoding RNAs formed through the alternative splicing of premessenger RNA (mRNA). (hindawi.com)
  • Polypyrimidine tract‐binding protein is essential for early mouse development and embryonic stem cell proliferation. (nature.com)
  • Luecken, M. D. & Theis, F. J. Current best practices in single-cell RNA-seq analysis: a tutorial. (nature.com)
  • McGinnis, C. S., Murrow, L. M. & Gartner, Z. J. DoubletFinder: doublet detection in single-cell RNA sequencing data using artificial nearest neighbors. (nature.com)
  • The lncRNA TMPO-AS1 promotes cell proliferation, migration, invasion and epithelial-to-mesenchymal transition by regulating the miR-1179/E2F2 axis [ 13 ]. (biomedcentral.com)
  • TPX2 regulated by miR-29c-3p induces cell proliferation in osteosarcoma via the AKT signaling pathway. (xenbase.org)
  • Nucleic acid-protein interactions regulate many aspects of gene expression and thus cell physiology and pathology. (cambridgeproteinarrays.com)
  • The present study was conducted to identify suitable RGs for microRNA expression studies in a human HCC-derived cell line (HepG2 tet-on), with and without HBV replication, after transfection of HBV expression vectors. (biomedcentral.com)
  • Our laboratory is interested in understanding the mechanisms by which microRNA and checkpoint proteins stall the cell cycle preventing genomic instability and cancer. (nii.res.in)
  • Aberrations in microRNA activity has been linked to loss of cell cycle and replication control and this has led to oncogenic functions (thereby called oncomirs) being ascribed to them. (nii.res.in)
  • A reduction in secreted reporter protein activity relative to a control cell into which the test polynucleotide has not been introduced is indicative that the test polynucleotide is capable of reducing the level of the target mRNA in the cells. (genomeweb.com)
  • Reliable and accurate gene expression quantification with subpopulation structure-aware constraints for single-cell RNA sequencing. (jhhlab.tw)
  • DeMarini, and Chapter 20, by Rice and cell death determine the size protein in several signal ing path- and Herceg). (who.int)
  • Subsequent development of in situ hybridization (ISH) techniques to detect EBV-encoded small RNAs (EBERs) facilitated the detection of EBV in cancer tissues ( 4 , 5 ). (spandidos-publications.com)
  • Due to the label-free single molecule detection without nucleic acids amplification, the nanopore sensor is higher selective, precise and accurate over the gold standard RT-PCR and microarray. (umsystem.edu)
  • EBVaGC is defined by monoclonal proliferation of carcinoma cells with latent EBV infection, as demonstrated by EBV-encoded small RNA (EBER) in situ hybridization. (spandidos-publications.com)
  • The small nuclear RNA U6 is frequently used as a reference gene (RG) in such studies [ 20 ]. (biomedcentral.com)
  • Target RNA-directed trimming and tailing of small silencing RNAs. (jhhlab.tw)
  • MicroRNA hsa-miR-199a is a regulator of IκB kinase-β (IKKβ) expression. (wikipedia.org)
  • Also, Twist-1, which is a major actor in skeleton formation, regulates miR-199 and miR-214 cluster expression in mouse. (wikipedia.org)
  • FSS also caused a time-dependent increase in the expression of miR-124a, a microRNA known to reduce GR mRNA expression, which was inversely correlated with a reduction in GR mRNA levels 30 min after FSS. (karger.com)
  • During his post-doctoral training under Dr. Paul Fox at Lerner Research Institute of Cleveland Clinic, he made contributions in understanding the translational control mechanisms regulating VEGFA (vascular endothelial growth factor-A) expression in human monocytes. (rochester.edu)
  • An increase in a cell's microRNA expression, therefore, means that a process is being inhibited. (fapesp.br)
  • Cardiac microRNA expression profile in response to estivation. (xenbase.org)
  • Altered expression levels of specific microRNAs are associated with hepatitis B virus infection and hepatocellular carcinoma. (biomedcentral.com)
  • This type of RNA is called a messenger RNA ( mRNA ), as it serves as a messenger between DNA and the ribosomes, molecular machines that read mRNA sequences and use them to build proteins. (khanacademy.org)
  • Plants have evolved numerous adaptive developmental and metabolic responses to cope with growth under phosphate-limited conditions, and these responses include modifying the root system architecture (RSA), increasing acid phosphatase activity (APA), and the release of low-molecular-weight organic acids [ 20 ]. (biomedcentral.com)
  • A post-transcriptional regulatory switch in polypyrimidine tract-binding proteins reprograms alternative splicing in developing neurons. (nature.com)
  • Direct conversion of fibroblasts to neurons by reprogramming PTB-regulated microRNA circuits. (nature.com)
  • The detrimental ramifications of altered microRNAs in AD neurons may possibly not be limited to A deposition and production. (bibf1120.com)
  • In this study, to explore the response mechanisms of lncRNAs to LP stress, we used the roots of two representative soybean genotypes that present opposite responses to P deficiency, namely, a P-sensitive genotype (Bogao) and a P-tolerant genotype (NN94156), for the construction of RNA sequencing (RNA-seq) libraries. (biomedcentral.com)
  • It is known that replication factors are downregulated during stress and we have observed that cullin 4-based ubiquitin ligase mediate the proteolysis of Mcm10, a protein essential for the initiation and elongation phases of DNA replication. (nii.res.in)
  • Traditional biomarkers of AMI include cardiac troponins and other proteins released from the injured myocardium but there are a number of limitations with these biomarkers especially with regard to specificity. (lancs.ac.uk)
  • Protein phosphatase 2A holoenzymes regulate leucine-rich repeat kinase 2 phosphorylation and accumulation. (xenbase.org)
  • MicroRNA can be linked to the phosphorylation and pathological aggregation of Tau proteins closely. (bibf1120.com)
  • Data from clinical trials indicate that miR-106b is down-regulated in sporadic AD patients and SH-SY5Y cells (31), and can inhibit A42-induced Tau phosphorylation at the site of Tyr18. (bibf1120.com)
  • DNA bears the hereditary information that's passed on from parents to children, providing instructions for how (and when) to make the many proteins needed to build and maintain functioning cells, tissues, and organisms. (khanacademy.org)
  • Bioinformatics analysis demonstrated that the EIF5B transcript and protein levels as well as the EIF5Bcopy number were significantly higher in the HCC tissues compared with the non-cancerous liver tissues. (techscience.com)