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  • detection
  • In addition to the recombinant DNA field, the analytical hybridization technique can be applied to the detection of polynucleotides of importance in the fields of human and veterinary medicine, agriculture, and food science, among others. (google.ca)
  • Nucleic acid sequence variations have been implicated in many diseases, and reliable detection and quantitation of DNA/RNA biomarkers can inform effective therapeutic action, enabling precision medicine. (nih.gov)
  • Improved methodologies for in-situ hybridization and non-isotopic detection of nucleic acid sequences are provided which offer major increases of resolution, sensitivity, and simplicity unavailable in previously known techniques. (google.com)
  • wherein
  • 10. A method according to claim 8 , wherein said oligonucleotide is indirectly linked to said solid phase by hybridization with said nucleic acid. (google.es)
  • 2. The method of claim 1 wherein before step(b) the resulting immobilized hybridized nucleic acids from the test medium are separated from the remainder of the reaction mixture. (google.ca)
  • 13. The method according to claim 3, wherein the fixative is a mixture of methanol and acetic acid in a 3:1 volumetric ratio. (google.com)
  • mixture
  • For example, all double-stranded nucleic acids - whether dsDNA, dsRNA or RNA:DNA hybrids - have specific 'melting temperatures', which depend mainly upon their specific guanine+cytosine content, but also upon whether they are DNA, RNA, or a mixture (RNA:RNA hybrids have the highest melting temperatures, followed by DNA:RNA hybrids, then dsDNA), and upon the ionic strength of solution. (uct.ac.za)
  • different
  • This polyvalency, along with the high density and degree of orientation described above, helps to explain why a three-dimensional nucleic acid structure fundamentally composed of linear, one-dimensional nucleic acids exhibits dramatically different properties than its lower-dimensional constituents (Fig. 2). (wikipedia.org)
  • Salt
  • nylon membranes (Hybond-N, GeneScreen) bind all nucleic acids under a wide range of salt concentrations, and irreversible or covalent attachment can be achieved by UV irradiation for 5 min or less, or by treatment with 0.4M NaOH. (uct.ac.za)
  • chemical
  • The inorganic nanoparticle core (in addition to gold, silver, iron oxide, silica, and semiconductor materials have also been used) serves two purposes: 1) it imparts upon the conjugate novel physical and chemical properties (e.g. plasmonic, catalytic, magnetic, luminescent), and 2) it acts as a scaffold for the assembly and orientation of nucleic acids into the dense arrangement that gives rise to many of their functional properties. (wikipedia.org)
  • In contrast, the SNA structure can be synthesized independent of nucleic acid sequence and hybridization, instead relying upon robust chemical bond formation between nanoparticles and nucleic acid adsorbates. (wikipedia.org)
  • further
  • Further, more exotic nucleic acid chemistries with oligonucleotide drugs may impact upon the activity of the ligase, which needs to be determined on a case-by-case basis. (wikipedia.org)
  • identify
  • Researchers Joseph Gall and Mary Lou Pardue realized in the 1960s that molecular hybridization could be used to identify the position of DNA sequences in situ (i.e., in their natural positions within a chromosome). (wikipedia.org)
  • tool
  • Since those original observations, many refinements have increased the versatility and sensitivity of the procedure to the extent that in situ hybridization is now considered an essential tool in cytogenetics. (wikipedia.org)