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  • coiled-coil peptides
  • Starting with an array of 81 sequence similar 32-residue coiled-coil peptides, we estimated the relative stability difference between all plausible A2B-type coiled-coil ensembles and used this information to predict the auto- and cross-catalysis pathways and the resulting plausible network motif and connectivities. (scripps.edu)
  • 72. Leman, L. J., Weinberger, D. A., Huang, Z.-Z., Wilcoxen, K. M., and Ghadiri, M. R. 'Functional and mechanistic analyses of biomimetic aminoacyl transfer reactions in de novo designed coiled coil peptides via rational active site engineering. (scripps.edu)
  • analogs
  • Several classes of nucleic acid analogs have been reported, but no synthetic informational polymer had previously proven responsive to selection pressures under enzyme free conditions. (scripps.edu)
  • This invention relates to peptide analogs of agouti polypeptide and agouti-related polypeptide that are useful in modulating feeding behavior. (google.com)
  • RNAs
  • The experimental application of RNA interference has become routine, but several new observations suggest that small RNAs might induce unappreciated cellular effects such as sequence specific transcriptional silencing or activation. (libpubmedia.co.uk)
  • biological
  • Likewise, automating processes for solid-phase synthesis (SPS) provided routine access to biological polymers such as peptides ( 3 ), nucleic acids ( 4 ), and polysaccharides ( 5 ) for a variety of fundamental and applied explorations in biotechnology, medicinal chemistry, and immunology. (pnas.org)
  • Nucleobases
  • It has been shown that appropriate pairs of pcPNAs hybridize efficiently to designated dsDNA targets containing all four nucleobases in a sequence-specific manner. (jbsdonline.com)
  • wherein
  • wherein said matrix comprises charged functional groups that are suitable for binding nucleic acid under electrostatic binding conditions. (google.com)
  • 5. The composition of claim 4 , wherein to the non-nucleotide probe/target sequence complex is directly or indirectly linked a detectable antibody. (google.com)
  • 3. The peptide of claim 2 wherein an acetyl group is used for acylation. (google.com)
  • 5. The device of claim 1 wherein said matrix comprises a material selected from the group consisting of collagen, hydroxyapatite, tricalcium phosphate, polymers comprising lactic acid monomer units, polymers comprising glycolic acid monomer units, demineralized, guanidine-extracted allogenic bone, and mixtures thereof. (google.com)
  • specificity
  • Further, this bioanalytical tool can open up a new paradigm in clinical settings for the screening of double-stranded circular nucleic acids with a single-base mismatch specificity in a rapid and sensitive manner. (springer.com)
  • affinity
  • Amino acid derivatives with sulfonic acid ester and sulfonamide linkages were identified as ASCT2 inhibitors with the most potent compound displaying an affinity in the low microM range. (binghamton.edu)
  • structures
  • The chiroselective amplication process discriminates between structures possessing even single stereochemical mutations within otherwise homochiral sequences. (scripps.edu)
  • Certain non-nucleoside compounds that will selectively inhibit telomerase by targeting the nucleic add structures, such as G-quadruplexes, that may be associated with human telomeres or telomerase have been identified. (google.com)
  • 1 H-NMR studies have evidenced the stabilization of a G-quadruplex by the perylenetetracarboxylic acid diimide compounds and provided evidence that these and structurally related compounds inhibit the telomerase enzyme by a mechanism consistent with interaction with G-quadruplex structures. (google.com)
  • Incorporating a variety of monomer structures and branching units provides control over polymer sequence and architecture. (pnas.org)
  • behavior
  • Our studies have ranged from defining simple condensation reagents that could have acted on the early Earth, to the development of replicating peptides that participate in chemical systems displaying behavior reminiscent of biology, such as symbiosis or parasitism. (scripps.edu)
  • mismatches
  • We investigated chloroplast sequence variation at the V4 locus across 500 land plant species (Embryophyta) and found six lineages with mismatches between plastid and the universal pPNA sequence, including all species within the Asteraceae. (biomedcentral.com)
  • Non-target nucleic acids, including single-base mismatches flanked by adenines and uracils, were removed with a micrococcal nuclease digestion step. (umaine.edu)
  • reaction
  • The Flow-IEG system performs three reactions and an in-line purification in a total residence time of under 10 min, effectively doubling the molecular weight of an oligomeric species in an uninterrupted reaction sequence. (pnas.org)
  • CHEMISTRY
  • They are assembled using techniques adapted from peptide chemistry. (currentprotocols.com)
  • at the 2016 Telluride Workshop on Nucleic Acid Chemistry in Telluride, CO. (binghamton.edu)
  • Herein, we report a general strategy for the scalable, semiautomated synthesis of macromolecules with uniform mass and precisely defined primary sequence by integrating iterative exponential growth (IEG) ( 8 ), an underused methodology in synthetic polymer chemistry, with modern multistep continuous flow synthesis ( 9 ). (pnas.org)
  • molecular
  • When high similarity exists between host organellar DNA and PCR target sequences, PNA clamps are an important molecular tool to reduce host contamination during amplification. (biomedcentral.com)
  • Qiang Group receives a major NIGMS grant to study the molecular basis of neuronal cell membrane disruption induced by early-stage aggregation of beta-amyloid peptides! (binghamton.edu)
  • telomerase
  • Inhibition of human telomerase by two perylenetetracarboxylic acid diimides and a carbocyanine has been demonstrated. (google.com)
  • simultaneously
  • Such high-host contamination limits the number of samples that can be sequenced simultaneously with sufficient read depth, and quickly erodes the value of next generation sequencing approaches in describing the microbiome. (biomedcentral.com)
  • chemical
  • Despite their promise, the efficient and scalable syntheses of unimolecular, sequence-defined, and nonbioresemblant polymers remains a major challenge for chemical synthesis ( 7 ). (pnas.org)
  • species
  • Using the universal PNA clamps, we amplified and sequenced root microbial communities from replicate individuals of 32 plant species with a most recent common ancestor inferred at 140 MYA. (biomedcentral.com)
  • reactions
  • in contrast to the previously observed error correction, it makes use of heterochiral sequences that arise through uncatalyzed background reactions to catalyze the production of the homochiral product. (scripps.edu)
  • Our approach made use of the sequence-dependant auto- and cross-catalytic functional characteristics of template-directed peptide fragment condensation reactions. (scripps.edu)
  • We have established the viability of reversible covalent reactions in water as a basis for template-directed synthesis of sequence-adaptive peptide nucleic acids. (scripps.edu)
  • Human
  • For example, automation of DNA sequencing ( 1 ) provided a user-friendly technology that helped unlock how hereditable information is passed between generations, eventually enabling the Human Genome Project ( 2 ). (pnas.org)
  • The corresponding human polypeptide is 132 amino acids in length, and is about 25 percent identical to human agouti polypeptide. (google.com)
  • cellular
  • For example, inside the cellular environment, signal transduction proceeds through a sequence of steps that transforms the system from one state to a completely different state. (springeropen.com)