Nucleic acid methods for sequencing by hybridization. Medical search. Frequent questions

Molecular beacons, or molecular beacon probes, are oligonucleotide hybridization probes that can report the presence of specific nucleic acids in homogenous solutions. (wikipedia.org)
They are useful in situations where it is either not possible or desirable to isolate the probe-target hybrids from an excess of the hybridization probes. (wikipedia.org)
5'-nuclease TaqMan assay Exciton-controlled hybridization-sensitive fluorescent oligonucleotide (ECHO) probes. (wikipedia.org)
Dual Hybridization (LightCycler®) probes Scorpions® Probes LUX (Light Upon Extension) Probes DNA binding dye assays (e.g. (wikipedia.org)
Development of commercially available molecular methods, such as nucleic acid hybridization probes, improved turnaround times significantly, but can lack specificity. (bruker.com)
Based on the knowledge and tools developed while identifying and characterizing new viruses and comparisons to previously-characterized viruses, new virus-specific and broad spectrum polyclonal and/or monoclonal antibody reagents, purification protocols, nucleic acid hybridization probes, PCR primers, isothermal amplification methods, and improved associated protocols will be developed. (usda.gov)
The problem is exacerbated if non-sequence-specific probes, such as pH-sensitive dyes, are used for the detection 6 . (nature.com)
Founded by University of Edinburgh researchers in 2010, Destina's core technology is based on pairing its aldehyde-modified Smart Nucleobases with peptide nucleic acid capture probes containing an abasic position that can be made to complement any nucleic acid system. (genomeweb.com)
Gene probes are used in various blotting and in situ hybridization (ISH) techniques for the detection of nucleic acid sequences in food industry, environmental, medical, and veterinary applications to improve the specificity of the analyses. (enzolifesciences.com)
Nucleic acid probes are either a single stranded DNA or RNA with a strong affinity towards a specific DNA or RNA target sequence. (enzolifesciences.com)
Probes labeled by nick translation can be used in many different hybridization techniques including: chromogenic in situ hybridization (CISH), fluorescent in situ hybridization (FISH), screening gene banks by colony or plaque hybridization, DNA or RNA transfer hybridization, and re-association kinetic studies. (enzolifesciences.com)
Hybridization capture-based enrichment, which uses small bits of nucleic acid termed oligonucleotides as capture probes, is one method that allows for targeted sequencing. (qsstudy.com)
However, while hybridization capture-based enrichment is an effective method for targeted sequencing, commercially produced biotinylated capture probes are costly and can only be utilized for a limited number of reactions, making the per-sample cost for each capture process prohibitive. (qsstudy.com)
To overcome this constraint, the CHOP researchers created TEQUILA-seq (Transcript Enrichment and Quantification Using Isothermal Linear-Amplified Probes in Conjunction with Long-Read Sequencing). (qsstudy.com)
The method is based upon the broadly-applicable principles of fluorescence-resonance energy transfer (FRET) and nucleic acid hybridization using fluorogenic oligonucleotide signaling probes originally reported for in tubo qRT-PCR applications, transfected into living cells. (genelink.com)
CHROMOVERT® PROCESS a) cDNAs are subcloned for expression of mRNAs comprising 3' untranslated plasmid-encoded Chromo-Tag™ sequences for detection using fluorogenic oligonucleotide signaling probes. (genelink.com)
This method is much more specific than Sybr Green, however the use of specific probes increases the cost considerably. (cuny.edu)
results can serve as an important argument in favour of the proposal to develop a special program for sequencing the genome chromosomes and for, To estimate the possibility of plant genome mapping using human genome probes, the probes fluorescent in situ hybridization (FISH) of human 18S-28S rDNA (clon 22F9 from the LA-13NCO1 library) was carried out on chromosomes of the spring barleyHordeum vulgareL. (centrocardiobogota.com)
DNA microarrays take advantage of the major feature of the DNA double helix, the sequence complementarity of the two-paired strands, by using DNA capture probes which are the complement of the expressed target sequence (mRNA, cRNA or cDNA made from the mRNA). (ddw-online.com)

A novel species-specific 14-bp PNA probe was designed for M. immunogenum based on its 16S rRNA gene sequence and was validated for specificity, by testing against a panel of other phylogenetically closely related rapidly growing mycobacteria and representative species of gram-positive, gram-negative, and acid fast organisms. (cdc.gov)
As detailed in the new paper, by pairing the Destina chemistry with Quanterix's platform, the investigators were able to develop a single-probe method for detecting miRNA from human serum using Quanterix's single-molecule arrays, which had the advantages of reading sequence specificity down to a single base without the need for amplification. (genomeweb.com)
We quantitatively detect two gene fragments, SARS-CoV-2 envelope (E) and open reading frame 1b (ORF1b), with high-specificity via DNA hybridization. (stanford.edu)
In terms of accuracy and specificity, RT-qPCR has become the method of choice for measuring gene expression levels, both for coding and non-coding RNAs. (biomedcentral.com)
Use these options to reveal the in silico specificity (i.e. number of matching rRNA sequences outside the target taxon) and coverage (i.e. percentage of matching rRNA sequences within the target taxon) of an oligonucleotide against the most recent SSU and LSU rRNA sequence databases. (univie.ac.at)

A method is described for visualizing three nucleic acid sequences simultaneously by in situ hybridization using a new blue immunofluorescent label, amino methyl coumarin acetic acid (AMCA), in combination with green and red fluorescing FITC and TRITC. (nih.gov)
Three-color in situ hybridization was applied to the study of numerical chromosome abnormalities as occur in human solid tumors. (nih.gov)
Peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) assay for specific detection of mycobacterium immunogenum and DNA-FISH assay for analysis of pseudomonads in metalworking fluid s and sputum. (cdc.gov)
Here, we report for the first time a species-specific peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) method for Mycobacterium immunogenum, a non-tuberculous Mycobacterium species prevalent in MWF and implicated in occupational lung disease hypersensitivity pneumonitis and pseudo-outbreaks. (cdc.gov)
Molecular diagnostic methods include microbial identification by nucleic acid amplification including 16S rRNA gene sequencing and real-time PCR, peptide nucleic acid fluorescence in situ hybridization, molecular methods for detection of antimicrobial resistance, bacterial strain typing, HPV DNA-typing and viral load testing and genotyping of HIV and HCV. (iu.edu)
Fluorescence in situ hybridization (FISH) is also an acceptable method, with ROS1positivity defined as the detection of at least 15% of neoplastic nuclei with ROS1 gene rearrangements among a minimum of 50 total neoplastic nuclei. (who.int)
New technologies such as in situ hybridization can be used to map the expression of multiple genes on the same tissue sample and have accelerated the generation of new atlases. (scienceboard.net)
In situ hybridization allows for a target gene to be tagged ("hybridized") with a fluorescent marker within sections of a tissue ("in situ") and visualized under a specialized microscope. (scienceboard.net)
The Tomographer framework was benchmarked for the ability to reconstruct transcriptome-wide spatial expression patterns against the Allen Adult Mouse Brain in situ hybridization atlas. (scienceboard.net)
Then, they compared 923 reconstructed genes to the in situ hybridization data from the mouse brain atlas using Pearson's correlation coefficient and found that the Tomographer workflow was more than twice as accurate as IPF. (scienceboard.net)
La Manno and his team found that the new sampling scheme/algorithm combination significantly outperformed previous in situ hybridization methods. (scienceboard.net)
The prognostic effects on IGBP1-AS1 survival were evaluated by using in situ hybridization and survival analysis. (biomedcentral.com)
Indeed, while methods of genetic testing such as in situ hybridization or detection tests such as protein mass spectrometry are widely used in the molecular diagnostic area, polymerase chain reaction remains one of the most fundamental techniques used to amplify specific sequences of DNA or RNA in molecular diagnostic tests [7]. (symbiosisonlinepublishing.com)

ISO 16578:2013 defines terms for the detection of nucleic acid sequence of interest using DNA microarrays for detection of nucleic acid. (iso.org)

Further applications of this method in prenatal diagnosis for the detection of aneuploidy of the most frequently involved autosomes, as well as for the quantification of gene copy number and mRNA expression, are discussed. (nih.gov)
The present invention relates to methods, kits and systems for predicting the benefit from inclusion of taxane in a chemotherapy regimen based on the measurements of gene expression levels in tumor samples of breast cancer patients. (justia.com)
A DNA probe is a labeled fragment of DNA that contains a nucleotide sequence specific for the gene or chromosomal region of interest. (enzolifesciences.com)
Using cheap reagents and an easy experimental workflow, TEQUILA-seq allows us to deeply sequence the full-length RNA molecules for any gene set across many biological samples," said co-senior author Yi Xing, Ph.D., head of CHOP's Center for Computational and Genomic Medicine. (qsstudy.com)
When tested in vitro, IB1 bound specifically to the GTII cis element of the GLUT2 gene and to an homologous regulatory sequence of the insulin promoter termed RIPE3. (justia.com)
Polymerase chain reaction (PCR), gene sequencing, microarrays, and other molecular techniques are used to identify specific genetic mutations, infectious agents, and biomarkers associated with diseases. (marketsandmarkets.com)
Alterations in gene sequence or expression can occur in the cell-signalling and regulatory pathways involved in cell-cycle control, apoptosis, proteosome regulation and angiogenesis. (bmj.com)
The ﬁrst section introduces sequence-basedanalyses,includinggeneﬁnd-ing, gene family and phylogenetic analy-ses, and comparative genomics approaches. (centrocardiobogota.com)
The subtraction method is a quick and economical technique to scan differential gene expression. (nih.gov)
Using the DTS method, we have successfully identified an 1812-bp additional GUS gene from the complicated Arabidopsis seedling cDNA library. (nih.gov)
April 21, 2021 -- A new framework called Tomographer, which uses sequencing data from tissues cut into thin strips in a way that allows them to be reconstructed, has been developed to spatially resolve gene expression data without the need for a microscope. (scienceboard.net)
And next-generation sequencing (NGS) libraries can be used to quantify spatial gene expression patterns that delineate between cell populations. (scienceboard.net)
In the current study, scientists at the Swiss Federal Institute of Technology Lausanne (EPFL) School of Life Sciences described how they have created a computational algorithm called Tomographer that can transform gene-sequencing data into spatially resolved data such as images, without using a microscope. (scienceboard.net)
Gene expression quantification of the sections is implemented using spatial transcriptomics by reoriented projections and sequencing (STRP-seq), a method that combines the sampling strategy presented above with a customized, low-input RNA-seq protocol based on single-cell tagged reverse transcription sequencing (STRT-seq) chemistry. (scienceboard.net)
The method produces parallel-slice projections for each gene by quantifying the reads that map to a transcript in each of the secondary sections. (scienceboard.net)
Molecular techniques like polymerase chain reaction (PCR) or gene sequencing. (stemcelldaily.com)
Although array CGH has provided a higher resolution compared to conventional CGH, it has not yet become a widely applied method for the analysis of gene dose alterations in individuals with idiopathic mental retardation. (bmj.com)
The rpoB gene target sequence was amplified using mutagenesis forward and reverse primers followed by Hin dIII enzyme digestion. (who.int)

Molecular beacons are hairpin-shaped molecules with an internally quenched fluorophore whose fluorescence is restored when they bind to a target nucleic acid sequence. (wikipedia.org)

Three specific biomolecular techniques are addressed: polymerase chain reaction, microarray comparative genomic hybridization, and whole-genome sequencing. (umd.edu)
This dissertation presents novel computational methods that unlock the potential of these diagnostics by efficiently analyzing whole-genome DNA sequences. (umd.edu)
Finally, multiple methods are presented for the validation of whole-genome sequence assemblies, which are capable of identifying assembly errors in even finished genomes. (umd.edu)
sequence) of the whole genome or its separate parts. (centrocardiobogota.com)
The rise of whole genome microbial sequencing: A new era for human microbiome analysis (on-demand webinar) Segre J, Pollard K. Science, Sep 29, 2017. (cdc.gov)

Gillespie and Spiegelman (1) observed that single stranded DNA binds strongly to nitrocellulose membranes in a manner that minimises the two strands reassociating with each other, but allows the hybridisation to complementary RNA. (ddw-online.com)
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. (bvsalud.org)
Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. (bvsalud.org)

Modern molecular biological methods (such as DNA cloning, gel electrophoresis, nucleic acid hybridization, PCR, and DNA sequencing) data analysis and interpretation. (charlotte.edu)
Existing technologies for targeted sequencing of full-length RNA molecules, on the other hand, are either expensive or difficult to set up, putting them out of reach for many labs," said co-senior author Lan Lin, Ph.D., Assistant Professor of Pathology and Laboratory Medicine at CHOP and a member of the Raymond G. Perelman Center for Cellular and Molecular Therapeutics. (qsstudy.com)
It specifies the verification processes and parameters for molecular biology analysis, including the detection and identification of specific nucleic acid sequences. (iso.org)
Molecular technique is further segmented into nucleic acid sequence based amplification and PCR. (prnewswire.co.uk)
Molecular diagnosis is currently based on a number of techniques to detect and quantify nucleic acids and specific proteins. (symbiosisonlinepublishing.com)
Standard chromosome analysis by G-banding has a limited resolution, but molecular cytogenetic techniques, such as multi-subtelomeric FISH, microdeletion FISH, multicolour FISH and comparative genomic hybridisation (CGH), have played an important role for the diagnosis of MR during the past decade. (bmj.com)
FIND) has partnered with Cepheid, Inc. (Sunnyvale, CA) and the University of Medicine and Dentistry of New Jersey (UMDNJ, Newark, NY) to develop a TB-specific automated, cartridge-based nucleic amplification assay (Xpert MTB/RIF) based on the GeneXpert multi-disease platform, currently unique in its simplification of molecular testing with fully integrated and automated sample preparation, amplification and detection required for real-time polymerase chain reaction. (bvsalud.org)

Current nucleic acid technologies such as polymerase chain reaction (PCR) and next-generation sequencing (NGS) rely on sample amplification and can suffer from inhibition. (stanford.edu)
Examples of acceptable methods include next-generation sequencing (NGS), Sanger sequencing, reverse transcriptase-polymerase chain reaction (RT-PCR), NanoString and EdgeSeq. (who.int)

The EPS matrix is made up of polysaccharides, proteins and nucleic acids. (ukessays.com)
Structural and functional interaction of nucleic acids and proteins in the replication, transcription and translation of genetic material. (charlotte.edu)

These validated assemblies provide the ultimate nucleic acid diagnostic, revealing the entire sequence of a genome. (umd.edu)
genome sequencing "essentially completed. (centrocardiobogota.com)
The completion of the sequencing of the human genome has uncovered 32,000 to 35,000 predicted genes, offering the prospect for many more drugable targets. (ddw-online.com)
Rare mutations in genome sequence that result from failed DNA repair are transmitted across generations through DNA replication during each cell division. (nature.com)
Unlike such genetic changes, epigenetic changes, which do not alter genome sequence, can result in three possible outcomes: passive dilution, active repair through negative feedback, or active maintenance through positive feedback. (nature.com)
The development of accurate and sensitive genome wide screening methods would facilitate the clinical diagnosis of patients with very small or subtle rearrangements. (bmj.com)

This Thesis reports on novel, ultrasensitive methods to detect specific NA sequences without the need for amplification of the target, e.g., using PCR. (dcu.ie)
Target amplification assays are based on the ability of complementary oligonucleotide primers to specifically anneal and allow enzymatic amplification of the target nucleic acid strands. (cdc.gov)

SNP detection Real-time nucleic acid detection Real-time PCR quantification Allelic discrimination and identification Multiplex PCR assays Diagnostic clinical assays Shekdar K, Langer J, Venkatachalan S, Schmid L, Anobile J, Shah P, et al. (wikipedia.org)
For designing real-time detection assays, an efficient data structure and search algorithm are presented to identify the most distinguishing sequences of a pathogen that are absent from all other sequenced genomes. (umd.edu)
For Lexington, Massachusetts-based Quanterix, which to date has deployed assays for protein analysis on its Single Molecule Array, or Simoa, platform, the approach demonstrates the applicability of its technology in nucleic acid detection, and miRNA detection in particular, as it prepares to launch a new system. (genomeweb.com)
Many new nucleic acid-based diagnostic tools or assays have been developed that allow analysis of DNA and RNA molecules in clinical samples. (enzolifesciences.com)
However, LCM assays are limited by laser-induced degradation of nucleic acids and the technical complexity of preparing multiple libraries. (scienceboard.net)

For microarray, novel pan-genomic design and analysis methods are presented for the characterization of unknown microbial isolates. (umd.edu)
Nucleic acid amplification diagnostics offer a rapid and accurate method of diagnosing microbial pathogens. (medscape.com)

To demonstrate the effectiveness of these methods, pan-genomic arrays are applied to the study of multiple strains of the foodborne pathogen, Listeria monocytogenes, revealing new insights into the diversity and evolution of the species. (umd.edu)

Primers for RT-LAMP or RT-RPA can be designed for specific viral RNA sequences, and the amplification methods are used before the Cas enzyme detection step, to multiply detection signals from the specific nucleic acid sequences. (nature.com)
Random priming is a type of primer extension in which a mixture of small oligonucleotide sequences, acting as primers, anneal to a heat-denatured double-stranded template. (enzolifesciences.com)

partial capsid sequencing showed different genetic types on dates when multiple samples were positive ( Figure ). (cdc.gov)
Genetic analysis methods are foundational to advancing personalized medicine, accelerating disease diagnostics, and monitoring the health of organisms and ecosystems. (stanford.edu)
Here, we introduce a label-free genetic screening platform based on high quality (high-Q) factor silicon nanoantennas functionalized with nucleic acid fragments. (stanford.edu)
Meet the Editor-in-Chief APPS's Editor-in-Chief, Dr. Theresa Culley (University of Cincinnati), studies the evolution of plant breeding systems and invasive species biology, using ecological and population genetic methods. (centrocardiobogota.com)
vi)a nucleic acid sequence according to any of (i) and (ii) which is degenerate as a result of the genetic code. (allindianpatents.com)
Other dependent claims added references to well-known genetic detection techniques that Canine EIC did not invent (e.g., amplification, hybridization, size analysis, sequencing, etc. (fredlaw.com)
Nucleic acids convey genetic information. (spilianakislab.gr)

Sequencing of target genes like 16S rDNA is very fast and highly accurate, but expensive and not widely available. (bruker.com)
Identify and characterize genes and/or phages affecting virulence and competitiveness of those bacteria to develop effective control methods. (usda.gov)
Targeted long-read RNA sequencing is an effective method for elucidating the RNA repertoire of any predefined set of genes. (qsstudy.com)
Users can customize the technique for various applications, and researchers can choose which genes to sequence and create the reagents for target capture in their own facilities. (qsstudy.com)
Transcriptional activator Islet-Brain 1 (IB1) is disclosed which is involved in the control of the GLUT2 and insulin genes by interacting with homologous cis-regulatory elements of the GLUT2 and insulin promoters, and to materials and methods deriving from this work. (justia.com)
With the human DNA probe, signals were detected in the major sites of the ribosomal genes on chromosomes 5I (7H) and 6I (6H) only when the chromosome preparations were obtained using an optimized technique with obligatory pepsin treatment followed by hybridization. (centrocardiobogota.com)
The above-mentioned ab, of repetitive sequences in plant genomes (up to 99%), makes difﬁcult the application of this technique for, mapping individual plant genes [40]. (centrocardiobogota.com)
The sequence analysis of the 32-35,000 predicted genes indicates the there may be as many as 1,000 kinase or kinase-like genes. (ddw-online.com)
This method was used to measure the number of copies of repeated genes such as rRNA genes (2) and to measure whether specific genes were under-replicated during the replication process used in forming polytene chromosomes (3). (ddw-online.com)
This technique requires preselection of target genes and sequencing methods with resolution limitations. (scienceboard.net)

Traditional methods are further segmented into microscopy, immunodiagnostics and biochemical characterization. (prnewswire.co.uk)
Although outbreaks of nonbacterial gastroenteritis were recognized as a public health concern, electron microscopy (EM) proved to be a tedious and insensitive method for routine examination for enteric viruses in stool specimens collected during outbreak investigations. (cdc.gov)

Use this tool to assess in silico sensitivity (i.e. the hybridization efficiency of the oligonucleotide with its fully complementary target sequence, calculated with ProbeMelt . (univie.ac.at)

The disc is pre-loaded with miRNA strands and each hybridization step is completed inside the disc by triggering the tabs of chambers to burst to release the contents. (dcu.ie)
The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (bvsalud.org)

For the application, Destina designed an abasic peptide nucleic acid probe for detecting miRNA with a reactive amine in lieu of a specific nucleic acid sequence. (genomeweb.com)

This is a novel non-radioactive method for detecting specific sequences of nucleic acids. (wikipedia.org)
Samples flagged as mycobacteria will undergo an optimized acquisition method and spectra are automatically compared to the MBT Mycobacteria IVD Library, for sensitive and specific identification. (bruker.com)
This affinity and complementary sequence allow binding to specific regions of a target sequence of nucleotides. (enzolifesciences.com)
The detection and quantitation of specific nucleic acid (NA) sequences continue to grow in importance. (dcu.ie)
When the hybridization is complete, the target miRNA which is specific to epilepsy, is detected based on the electrocatalytic reduction of hydrogen peroxide in phosphate buffer saline (PBS) at the PtNPs which are functionalised to the probe miRNA strand. (dcu.ie)
In the presence of target sequence, hybridization of the sequence-specific probe results in a fluorogenic conformational change. (genelink.com)
This method, duplex-specific nuclease (DSN)-mediated transcriptome subtraction (DTS), is based on the normalization strategy of the crab duplex-specific nuclease and the subtraction method of suppression subtractive hybridization. (nih.gov)
The capture oligomers contain sequences complementary to specific regions of the target molecules as well as a string of deoxyadenosine residues. (cdc.gov)
During the hybridization step, the sequence specific regions of the capture oligomers bind to specific regions of the target molecules. (cdc.gov)

The result is a dynamic chemistry approach that relies on a chemical, rather than enzymatic, method for nucleic acid testing. (genomeweb.com)

Policy statement: automated real-time nucleic acid amplification technology for rapid and simultaneous detection of tuberculosis and rifampicin resistance: Xpert MTB/RIF system. (bvsalud.org)

The duplex formed between the nucleic acid and the loop is more stable than that of the stem because the former duplex involves more base pairs. (wikipedia.org)
ii) contacting any probe/target nucleic acid duplex with an exonuclease to effect digestion of the duplex and release of a label molecule from the duplex: and (ii) detecting the label by Raman spectroscopy. (strath.ac.uk)
To address these unknowns, we employ steady-state and time-resolved infrared spectroscopy to measure the influence of cytosine modification on the thermodynamics and kinetics of hybridization by assessing the impact on local base pairing dynamics, shifts in the stability of the duplex state, and changes to the hybridization transition state. (acs.org)

This dissertation focuses on computational methods for improving the accuracy of commonly used nucleic acid tests for pathogen detection and diagnostics. (umd.edu)
These methods are potentially the future of diagnostics, but each requires sophisticated computational design or analysis to operate effectively. (umd.edu)
Children's Hospital of Philadelphia (CHOP) researchers have created a versatile and low-cost technology for targeted sequencing of full-length RNA molecules, which could speed up the discovery of new diagnostics and therapies. (qsstudy.com)

The three objectives of this project are: (1) Characterize viruses of major significance to ornamental and nursery crops, including uncharacterized or emerging viruses affecting key ornamental crops, and develop corresponding diagnostic testing methods. (usda.gov)

Results are presented that show these "signature" sequences can be used to detect pathogens in complex samples and differentiate them from their non-pathogenic, phylogenetic near neighbors. (umd.edu)
We also employed DTS to detect the differences in mRNA expression of salt-treated Arabidopsis seedlings to illustrate further the efficiency of the subtraction method. (nih.gov)

These oligonucleotides (also known as "oligos") are biotin-tagged and intended to hybridize to their targets based on nucleic acid sequence complementarity, allowing enabling simple capture and isolation of target sequences from biological samples. (qsstudy.com)

Dot blotting and dot hybridisation (4) evolved out of the filter hybridisation technique and provided the basic concept for DNA arrays. (ddw-online.com)

However, most subtraction methods are limited by the complexity and length of cDNA samples. (nih.gov)
DSN eliminates nearly all of the common sequences in the tester and driver cDNA samples after the first hybridization step, ensures accurate discrimination between the tester and the driver cDNA samples, and enriches the full-length differential cDNAs from the tester. (nih.gov)

XX DT 05-MAY-2009 DT 26-AGO-2011 XX DE Qualitative PCR method for detection of chloroplast tRNA-Leu intron DE (ISO/FDIS 21569:2005). (europa.eu)

initially the capture NA strand is immobilised onto the electrode surface, this is then hybridised to the complementary target sequence, which is then followed by target hybridisation to a complementary probe strand which is labelled with a nanoparticle. (dcu.ie)

And for scientists at the University of Edinburgh, it offers the hope of introducing miRNA testing that is faster than PCR and less expensive than next-generation sequencing. (genomeweb.com)
The probe was coupled to Quanterix's superparamagnetic beads, and the beads were then incubated with a sample containing miRNA, a reactive nucleobase that complemented the missing base in the probe sequence, and a reducing agent. (genomeweb.com)

The threshold CT values obtained from the curve are used to calculate relative quantification by comparative âˆ†âˆ†CT method. (ukessays.com)

The IB1cDNA encodes a 714 amino acid protein with a proline-rich region and a putative basic helix-loop-helix domain (bHLH). (justia.com)

When using this method of diagnosis, specimen collection must occur prior to acetic acid or iodine use if colposcopy is to follow. (medscape.com)

Improved detection and differentiation methods for these pathogens will enable state and federal regulatory officials to make timely and appropriate recommendations in safeguarding the movement of horticultural and agricultural products into the United States. (usda.gov)
Important cis-regulatory sequences were identified within this promoter region including a functionally responsive PDX-1 element, a cyclic AMP responsive element, and three cis elements termed GTI, GTII and GTIII (3,36,53). (justia.com)
Here we report that a minimal combination of cis- regulatory sequences can support permanent RNA silencing of a single-copy transgene and its derivatives in C. elegans simply upon mating. (nature.com)
Recovery is also observed in most cases when double-stranded RNA is used to silence the same coding sequence in different regulatory contexts that drive germline expression. (nature.com)

Strategies for normalization of high-dimensional expression profiling experiments (using, for example, microarray technology, but recently also transcriptome sequencing) generally take advantage of the huge amount of data generated and often use (almost) all available data points. (biomedcentral.com)

This stable silencing requires components of the small RNA pathway and can silence homologous sequences in trans . (nature.com)

The relative quantification method of qPCR or real time PCR method does not require any standard curve for the analysis where the samples are normalised by using an internal control. (ukessays.com)
Designing small universal k-mer hitting sets for improved analysis of high-throughput sequencing. (cdc.gov)
The results showed that those 2 isolates were multi-drug resistant and the DNA sequencing analysis showed that the alignment of nucleic acid of DNA in isolates of mycobacteria other than M. tuberculosis was different from that of M. tuberculosis complex. (who.int)

Traditional methods for mycobacteria identification, primarily culture-based phenotypic methods, may take weeks and are often inaccurate. (bruker.com)

Early studies of DNA melting and reformation were carried out in aqueous solutions and yielded important information about the dependence of melting temperature (Tm) on the G+C composition and salt concentration, as well as information on the dependence of the rate of reassociation on the sequence complexity of the nucleic acid. (ddw-online.com)

Next generation sequencing (NGS) of nucleic acid extracts from plants infected with unknown viruses is expected to yield information about the genomes of previously uncharacterized viruses without any background information on what viruses might be infecting the plant. (usda.gov)

Mutation and recombination in pathogen evolution: Relevance, methods and controversies. (cdc.gov)

The degree of homology between target and probe results in stable hybridization. (enzolifesciences.com)

Epidemiology of transmissible diseases: Array hybridization and next generation sequencing as universal nucleic acid-mediated typing tools. (cdc.gov)

Canine EIC Genetics obtained U.S. Patent No. 8,178,297 - titled "Method of Detecting Canine Exercise-Induced Collapse" - in May 2012. (fredlaw.com)

Anatomy12

Organisms7

Diseases4

Chemicals and Drugs77

Analytical, Diagnostic and Therapeutic Techniques and Equipment55

Phenomena and Processes105

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Probes19

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Gene18

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