• Diagnostics based on nucleic acid sequence variant profiling: PCR, hybridization, and NGS approaches. (nih.gov)
  • Nucleic acid sequence variations have been implicated in many diseases, and reliable detection and quantitation of DNA/RNA biomarkers can inform effective therapeutic action, enabling precision medicine. (nih.gov)
  • or whether the nucleic acid sequence of interest is localized in the chromosomes, nucleus, or cytoplasm of a cell. (google.com)
  • Devices and techniques for hybridization of nucleic acids and for determining the sequence of nucleic acids. (patents.com)
  • Devices and techniques are proposed to determine the sequence of a target nucleic acid more efficiently and more quickly through such synthesis and detection techniques. (patents.com)
  • Nick Translation Nick Translation: Involves the synthesis and labeling of a Probe A piece of Nucleic Acid labeled (incorporated) with a Tracer that allows one to 'track' the hybridization of the Probe to an unknown sequence of DNA A Tracer could be: 1. (coursehero.com)
  • Thus, the association of two nucleic acid molecules - presumed to be at least a few hundred bases long - is an extremely sequence-specific process, far more so than the widely-used specificity of monoclonal antibodies in binding to specific antigenic determinants. (uct.ac.za)
  • To the N-terminus of a nonamer peptide nucleic acid sequence, H-GCACGACTT-NH2, was attached a number of lipophilic conjugate molecules including three synthetic tocopherol (vitamin E) analogues. (edu.au)
  • 1. An isolated and substantially pure polypeptide, comprising a polypeptide having a purity of greater than 75% by weight and a molecular weight of about 700 to about 4000 daltons and having the amino acid sequence Gln-Gln-Ser-Tyr-Ser-Ile-Met-Ser-Ile-Ile-Lys-Glu-Glu-Val-Leu-Ala-Tyr-Val-Val-Gln-Leu-Pro-Leu-Tyr-Gly-Val-Ile-Asp-Thr-Pro-Cys-Trp-Lys. (google.co.uk)
  • 3. An isolated and substantially pure polypeptide, comprising a polypeptide having a purity of greater than 75% by weight and a molecular weight between about 700 to about 2500 daltons and having the amino acid sequence Glu-Glu-Val-Leu-Ala-Tyr-Val. (google.co.uk)
  • Chapter 07 - Nucleic Acid Hybridization: Principles and Applications (Human Molecular Genetics) 4th Edition by Tom Strachan, Andrew Read and Publisher Garland Science. (vitalsource.com)
  • In contrast, buffers such as glycine, β-alanine and γ-amino-butyric acid (GABA) produce rapid transport and site selective concentration but do not facilitate hybridization. (elsevier.com)
  • Several approaches, such as microarray hybridization, have become extremely popular tools for specialists in biochemistry and biomedicine, while the potential of many other advantageous techniques seems to be underestimated. (indigo.ca)
  • 2. The method of claim 1 wherein before step(b) the resulting immobilized hybridized nucleic acids from the test medium are separated from the remainder of the reaction mixture. (google.ca)
  • The global standard library of rRNA sequences is constantly becoming larger and continuously being updated, and thus the possibility of a random hybridization event between a specifically-designed probe (based on complete and current data from a range of test organisms) and an undesired/unknown target organism cannot be easily dismissed. (wikipedia.org)
  • For example, all double-stranded nucleic acids - whether dsDNA, dsRNA or RNA:DNA hybrids - have specific 'melting temperatures', which depend mainly upon their specific guanine+cytosine content, but also upon whether they are DNA, RNA, or a mixture (RNA:RNA hybrids have the highest melting temperatures, followed by DNA:RNA hybrids, then dsDNA), and upon the ionic strength of solution. (uct.ac.za)
  • The protein product of FDH shows similarity to condensing enzymes involved in lipid biosynthesis, particularly those of the FATTY ACID ELONGATION family. (plantcell.org)