• Understanding the binding specificity of a DNA binding protein is helpful for the research of protein functions. (biomedcentral.com)
  • Therefore, a computational method is required to annotate the DNA binding protein as DSB or SSB automatically. (biomedcentral.com)
  • Pur-alpha is a highly conserved, sequence-specific DNA- and RNA-binding protein involved in diverse cellular and viral functions including transcription, replication, and cell growth. (embl-heidelberg.de)
  • The retinoblastoma protein, Rb, is detected in extracts of monkey CV-1cells complexed with Pur alpha, a sequence-specific single-strandedDNA-binding protein implicated in control of gene transcription and DNAreplication. (embl-heidelberg.de)
  • In this platform, catalytically inactive Cas9 serves as an RNA-guided DNA binding protein to silence gene expression in a sequence-specific manner. (berkeley.edu)
  • Φ29 is a bacteriophage of Bacillus subtilis with a sequenced, linear, 19,285 base pair DNA genome. (wikipedia.org)
  • This was demonstrated by the ability of the enzyme to continue to copy the singly primed circular genome of the M13 phage more than tenfold in a single strand (over 70kb in a single strand). (wikipedia.org)
  • Virus particles have a nucleic acid genome and may also carry a few viral proteins, which are enclosed in a capsid of viral proteins or an envelope comprised of a small part of the host cell membrane. (fortunejournals.com)
  • The genome consists of either RNA or DNA and can be single- or double-stranded, linear or circular. (fortunejournals.com)
  • The CRISPR-Cas9 system can be used to quickly and specifically target and cleave DNA at sites defined by engineered single-guide RNAs (sgRNAs) and has led to its adoption as a robust and versatile platform for genome engineering. (universityofcalifornia.edu)
  • These kits are single-box solutions for sequencing the entire viral genome. (qiagen.com)
  • The QIAseq DIRECT SARS-COV-2 Kit is a single-box solution for generating whole genome libraries that are sequencing-ready and compatible with all Illumina platforms. (qiagen.com)
  • Viruses consist of nucleic acid (viral genome) and a limited number of proteins that facilitate entry into a host cell, replication of the genome and production of virions. (qiagen.com)
  • For example, consider a very simple genome of 5 kb, enough to encode about 1,600 amino acid residues, if reading frames do not overlap. (basicmedicalkey.com)
  • The shell of a nonenveloped virus with even a very small genome must therefore contain a large number of identical protein subunits-at least 60, if the coat-protein gene is to use up less than 25% of the coding capacity in the enclosed nucleic acid. (basicmedicalkey.com)
  • The Class 2 CRISPR-Cas system, which comprises the type II, V, and VI CRISPR-Cas systems, consists of a single effector nuclease, and routine practise of genome editing has been achieved, thanks to the development of the Class 2 CRISPR-Cas system. (experiome.com)
  • On 23 pairs of chromosomes, the human genome comprises around 3 billion nucleotides and 20,000 genes. (javatpoint.com)
  • HPV is the etiological agent responsible for more than 99% of all cervical cancers.1, 2, 3 HPV is a common sexually transmitted DNA virus comprised of more than 100 genotypes.1 The HPV viral genome is a double-stranded circular DNA approximately 7900 base pairs in length. (clinicalpathologyassociates.com)
  • Positive-sense RNA viruses possess a single-stranded RNA genome that can serve as messenger RNA (mRNA) that can be directly translated to produce an amino acid sequence. (msdmanuals.com)
  • Negative-sense RNA viruses possess a single-stranded negative-sense genome that first must synthesize a complementary positive-sense antigenome, which is then used to make genomic negative-sense RNA. (msdmanuals.com)
  • Retroviruses use reverse transcription to create a double-stranded DNA copy (a provirus) of their RNA genome, which is inserted into the genome of their host cell. (msdmanuals.com)
  • the genome of RNA viruses ranges from 3.5 kilobases (some retroviruses) to 27 kilobases (some reoviruses), and the genome of DNA viruses ranges from 5 kilobases (some parvoviruses) to 280 kilobases (some poxviruses). (msdmanuals.com)
  • DNA binding proteins can be classified into double-stranded DNA binding proteins (DSBs) and single-stranded DNA binding proteins (SSBs), and they take part in different biological functions. (biomedcentral.com)
  • Experimental results on 10-fold cross validation indicate that the new feature set are effective to describe DNA binding proteins. (biomedcentral.com)
  • The evaluation results on both bound (DNA-bound) and non-bound (DNA-free) proteins have shown the satisfactory performance of our method. (biomedcentral.com)
  • Accordingly, the DNA binding proteins usually consist of single-stranded DNA-binding proteins (SSBs) and double-stranded DNA-binding proteins (DSBs). (biomedcentral.com)
  • Prokaryotic Argonaute proteins are typically comprised four domains: the MID domain, the PAZ domain, the PIWI domain, and the N domain. (neb.com)
  • Although some virion-associated proteins such as the major capsid protein, comprise structural elements of the particle, others serve catalytic or regulatory roles, e.g., the virion-associated transactivator of immediate-early transcription. (ictv.global)
  • Nucleic-acid therapies use a class of emerging drugs including ASOs that identify genes at the genetic level by suppressing the expression of pathogenic proteins. (newswingz.com)
  • DNA/RNA-binding repeats in PUR-alpha/beta/gamma and in hypothetical proteins from spirochetes and the Bacteroides-Cytophaga-Flexibacter bacteria. (embl-heidelberg.de)
  • One of the ways proteins are able to repair damaged DNA is that they can bind to uneven spots within the structure. (onelovefilm.com)
  • Glass or quartz nanopipettes have found increasing use as tools for studying the biophysical properties of DNA and proteins, and as sensor devices. (imperial.ac.uk)
  • DNA-templated assembly was shown to enable the patterning of proteins and create conductive nanowires ( 2 ), and the complexity of these systems increased with landmark demonstration of structures like the DNA octahedron folded from 1.7-kb single-stranded (ss)DNA ( 3 ). (allenpress.com)
  • DNA sequences that form the coding region for the viral envelope (env) proteins in retroviruses. (lookformedical.com)
  • DNA repair proteins have demonstrated promising capabilities in this area, but putting these ideas into practise in vivo is difficult. (experiome.com)
  • The DNA strands that comprise each gene are blueprints for producing specific proteins. (javatpoint.com)
  • Although patients may differ in their clinical manifestations, patients do share a propensity to develop autoantibodies directed against nucleic acids and associated nuclear and cellular proteins. (cdc.gov)
  • HIV infection can be diagnosed based on detection of antibodies that are directed against the proteins encoded by the 3 major genes, the detection of the p24 antigen, the viral nucleic acid, and, finally, by means of culturing the virus. (medscape.com)
  • Once the provirus is integrated into the host cell DNA, it is transcribed using typical cellular mechanisms to produce viral proteins and genetic material. (msdmanuals.com)
  • Each 5' end is covalently linked to a terminal protein, which is essential in the replication process by acting as a primer for the viral DNA polymerase. (wikipedia.org)
  • DSBs usually act as transcriptional factors to regulate the genes' expressions, while SSBs usually play roles in DNA replication, recombination, and repair, etc. (biomedcentral.com)
  • SSB binds with ssDNA with high affinity and low specificity, and is mainly involved in DNA replication, recombination and repair. (biomedcentral.com)
  • The human Pur factor binds strongly to a sequence element repeated withinzones of initiation of DNA replication in several eukaryotic cells. (embl-heidelberg.de)
  • Replication of deoxyribonucleic acid (DNA) marks the initiation of the binary fission process. (biologywise.com)
  • The process of replicating nucleic acid begins from a replication origin. (biologywise.com)
  • Following this, a replication bubble is formed, which separates the DNA strands. (biologywise.com)
  • It is active during DNA replication, DNA repair and DNA recombination. (embl.de)
  • Some viruses have an outer envelope consisting of protein and lipid, surrounding a protein capsid complex with genomic RNA or DNA and sometimes enzymes needed for the first steps of viral replication. (msdmanuals.com)
  • Certain single-strand, (+) sense RNA viruses termed retroviruses use a very different method of replication. (msdmanuals.com)
  • Sigma-Aldrich, Saint Louis, MO, USA), which was developed for amplification of genomic DNA, was used to amplify viral double-stranded cDNA. (cdc.gov)
  • In this study we perform tumor and normal whole exome sequencing (WES) on 32 pediatric primary MDS patients and targeted sequencing on another 14 cases through a single institution study focused on defining the genomic landscape of pediatric MDS. (nature.com)
  • Facilitates rearangement of nucleic acid secondary structure during retrotranscription of genomic RNA. (proteopedia.org)
  • Furthermore, DSBs in cells caused by DNA repair mechanisms are reported, which result in a variety of undesirable genomic changes such as significant deletions and translocations. (experiome.com)
  • Synthesis is continual and involves a strand displacement mechanism. (wikipedia.org)
  • The continuous nature of strand synthesis (compared to the asymmetric synthesis seen in other organisms) probably contributes to this enhanced processivity. (wikipedia.org)
  • Highly efficient DNA synthesis by the phage Φ29 DNA polymerase. (wikipedia.org)
  • Claim 16: A pair of single-stranded DNA primers for determination of a nucleotide sequence of a BRCA1 gene by a polymerase chin reaction, the sequence of said primers being derived from human chromosome 17q, wherein the use of said primers in a polymerase chain reaction results in the synthesis of DNA having all or part of the sequence of the BRCA1 gene. (patentdocs.org)
  • A pair of single-stranded DNA primers of at least 15 nucleotides in length for determination of the nucleotide sequence of a BRCA2 gene by a polymerase chain reaction, the sequence of said primers being isolated from human chromosome 13, wherein the use of said primers in a polymerase chain reaction results in the synthesis of DNA comprising all or at least 15 contiguous nucleotides of the BRCA2 gene. (patentdocs.org)
  • The RDV method comprises 6 procedures: 1) effective destruction of cellular RNA and DNA for semipurification of viral particles, 2) effective elimination of DNA fragments by using a prefiltration column system and elution of small amounts of RNA, 3) effective synthesis of first- and second-strand cDNAs, 4) construction and amplification of a cDNA library, 5) construction of a second cDNA library, and 6) direct sequencing using optimized primers. (cdc.gov)
  • Then 60 U of RNase H (Takara Bio Inc.) added before synthesis of second-strand cDNA at 50°C for 1 h. (cdc.gov)
  • Each of the strands then serve as a template for synthesis of the complementary strand. (biologywise.com)
  • Here we describe the synthesis and properties of the first family of molecules belonging to this compound category-differently sized rings comprising only 1,1′-disubstituted ferrocene units (cyclo[n], n = 5-7, 9). (imperial.ac.uk)
  • and CST complex with respect to the regulation of telomere G and C-strand synthesis. (grantome.com)
  • and define the mechanisms by which CST stimulates telomere C-strand synthesis in vitro and in vivo. (grantome.com)
  • The anticipated outcome is a deeper understanding of mechanisms that regulate telomere DNA synthesis, which should inform the development of telomere-based clinical applications. (grantome.com)
  • This research will offer greater understanding of the mechanisms of telomere DNA synthesis, which will in turn catalyze the development of new diagnostics and therapies for telomere-related disorders. (grantome.com)
  • RT uses the 3' end of the tRNA primer to perform a short round of RNA-dependent minus-strand DNA synthesis. (proteopedia.org)
  • RT uses the 3' end of this newly synthesized short ssDNA to perform the RNA-dependent minus-strand DNA synthesis of the whole template. (proteopedia.org)
  • RNase H probably can proceed both in a polymerase-dependent (RNA cut into small fragments by the same RT performing DNA synthesis) and a polymerase-independent mode (cleavage of remaining RNA fragments by free RTs). (proteopedia.org)
  • Secondly, RT performs DNA-directed plus-strand DNA synthesis using the PPTs that have not been removed by RNase H as primers. (proteopedia.org)
  • Φ29 DNA polymerase is an enzyme from the bacteriophage Φ29. (wikipedia.org)
  • it comprises a C-terminal polymerase domain and a spatially separated N-terminal domain with a 3'-5' exonuclease activity. (wikipedia.org)
  • This enzyme displays a DNA polymerase activity that can copy either DNA or RNA templates, and a ribonuclease H (RNase H) activity that cleaves the RNA strand of RNA-DNA heteroduplexes in a partially processive 3' to 5' endonucleasic mode. (proteopedia.org)
  • All organisms-bacteria, archaea, and eukaryotes-have a transcription initiation factor that contains a structural module that binds within the RNA polymerase (RNAP) active-center cleft and interacts with template-strand single-stranded DNA (ssDNA) in the immediate vicinity of the RNAP active center. (osti.gov)
  • Transcription-mediated amplification (TMA) is an isothermal, single-tube nucleic acid amplification system utilizing two enzymes, RNA polymerase and reverse transcriptase , to rapidly amplify the target RNA/DNA, enabling the simultaneous detection of multiple pathogenic organisms in a single tube. (clinicalpathologyassociates.com)
  • Proofreading activity conferred by the exonuclease domain was demonstrated by showing the preferential excision of a mismatched nucleotide from the 3' terminus of the newly synthesized strand. (wikipedia.org)
  • Three parts of a nucleotide - a building block of nucleic acids (DNA and RNA) DNA and RNA are polymers. (onelovefilm.com)
  • A nucleotide is an organic molecule that is the building block of DNA and RNA. (onelovefilm.com)
  • Eight single nucleotide polymorphisms (SNP) in the NAT2 coding region and a variant in the 3′ flanking region were analyzed in 290 unrelated Kyrgyz and 140 unrelated Romanians by SNP-specific PCR analysis. (aacrjournals.org)
  • Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. (lookformedical.com)
  • DNA damage must be repaired by one of four cellular processes, base excision repair, nucleotide excision repair, mismatch repair, or double-strand break repair, and alterations in the repair process can allow DNA damage to accumulate [ 1 ]. (hindawi.com)
  • A gene is made up of a lengthy combination of four distinct nucleotide bases, which are the fundamental units of DNA. (javatpoint.com)
  • We conducted a meta-analysis of four independent European SLE case collections for associations between SLE sub-phenotypes and MHC single-nucleotide polymorphism genotypes, human leukocyte antigen (HLA) alleles and variant HLA amino acids. (cdc.gov)
  • 13 Our study substantially expands this work by not only analysing imputed classical human leukocyte antigen (HLA) alleles, but also examining variant HLA amino-acid positions in conjunction with single-nucleotide polymorphism (SNP) genotypes across the extended MHC region (chromosome 6: 26-34 Mb). (cdc.gov)
  • In this video, learn more about argonautes, including how these nucleic acid-guided endonucleases differ from Cas enzymes and potential applications. (neb.com)
  • Cas enzymes have two active sites, so they're able to cut in two places on a double-stranded substrate. (neb.com)
  • DNA sequences that form the coding region for retroviral enzymes including reverse transcriptase, protease, and endonuclease/integrase. (lookformedical.com)
  • In another embodiment, serine-67 is present in a peptide of less than 30 amino acids that comprises the sequence of SEQ ID NO. 4. (justia.com)
  • In another embodiment, the peptide comprising the sequence of SEQ ID NO. 4 is less than 25 amino acids in length. (justia.com)
  • Preferably, this peptide is less than 20 amino acids in length. (justia.com)
  • In a more preferred embodiment, the peptide comprising the sequence of SEQ ID NO. 4 is 18 amino acids long. (justia.com)
  • The order of amino acids as they occur in a polypeptide chain. (lookformedical.com)
  • There are two kinds of DNAs, single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). (biomedcentral.com)
  • While DSBs involve in binding to particular dsDNA sequences, to modulate the process of transcription, to cleave DNA molecules, or to be involved in chromosome packaging and transcription in the cell nucleus, etc. (biomedcentral.com)
  • Cyclic oligomers comprising strongly interacting redox-active monomer units represent an unknown, yet highly desirable class of nanoscale materials. (imperial.ac.uk)
  • Although Sec complexes can form oligomers, it has been suggested that a single copy may serve as an active PCC. (cipsm.de)
  • The molecular biology of CRISPR/Cas demonstrates how it can be used to detect disease-causing genetic variants by applying synthetic guide RNAs (gRNAs) and other components to the target region of interest in a DNA molecule for the desired application. (experiome.com)
  • It is being increasingly used in molecular biology for multiple displacement DNA amplification procedures, and has a number of features that make it particularly suitable for this application. (wikipedia.org)
  • multiple-strand displacement amplification. (wikipedia.org)
  • The MID domain of prokaryotic Argonaute binds a short single stranded oligonucleotide guide, which is typically 16 to 18 nucleotides in length. (neb.com)
  • The R3H motif: a domain that binds single-stranded nucleic acids. (embl-heidelberg.de)
  • Inthe absence of DNA, glutathione S-transferase-Pur alpha binds to p56RB, anNH2-terminal-truncated Rb protein purified from Escherichia coli,containing the T-antigen binding domain, to form multimeric complexes. (embl-heidelberg.de)
  • Theprotein binds preferentially to the purine-rich single strand of thiselement, PUR. (embl-heidelberg.de)
  • Accordingly, the present invention envisions an isolated nucleic acid encoding the GEF-H1S polypeptide of SEQ ID NO:2, as well as an isolated nucleic acid comprising the sequence of SEQ ID NO:1. (justia.com)
  • In this context, the invention also contemplates an isolated GEF-H1S polypeptide comprising the sequence of SEQ ID NO:2. (justia.com)
  • In a preferred embodiment, a GEF-H1 polypeptide is provided that lacks the amino acid region between residues 162 and 354 of SEQ ID NO. 2. (justia.com)
  • The whole nucleic acid sequence required for the production of a functional gene product, which can be a polypeptide or any RNA, is what Lodish and others refer to as a gene. (javatpoint.com)
  • Prokaryotic Argonautes are nucleic acid guided endonucleases involved in prokaryotic cellular defense against foreign genetic elements. (neb.com)
  • To eliminate contaminating cellular RNA and DNA from the samples, 0.001 μg of RNase A (Qiagen, Hilden, Germany) and 1 μL (2 U) of Turbo DNA-free DNase I (Ambion, Austin, TX, USA) with 1× Turbo DNA-free buffer were incubated at 37°C for 30 min under conditions that prevented destruction of viral RNA in the viral particles. (cdc.gov)
  • Nucleotides are the building blocks of DNA and RNA molecules. (onelovefilm.com)
  • Based on the famous ""mfold"", the UNAFold software package is an integrated collection of programs that simulate nucleic acid folding and hybridization, and its melting pathways for one or two single-stranded molecules. (rpi.edu)
  • The technique called DNA origami, invented in 2006 by Paul Rothemund ( 4 ), demonstrated a computationally simple way to build multiple kilobase-scale structures from a large ssDNA "scaffold" and hundreds of small ssDNA "staples. (allenpress.com)
  • The portion of the RNA-DNA heteroduplex is digested by the RNase H, resulting in a ssDNA product attached to the tRNA primer. (proteopedia.org)
  • This transcription initiation-factor structural module preorganizes template-strand ssDNA to engage the RNAP active center, thereby facilitating binding of initiating nucleotides and enabling transcription initiation from initiating mononucleotides. (osti.gov)
  • The object of the present report is to provide a method for a visualization of DNA in TEM by complementary labeling of cytosine with guanine derivative, which contains platinum as contrast-enhanced heavy element. (ung.si)
  • Guanine quadruplexes are nucleic acid structures built ions in slow exchange with bulk ions. (lu.se)
  • In the absence of a repair template, however, DSBs are repaired by the NHEJ pathway, which introduces insertions or deletions by editing the DNA region, causing target genes to be disrupted by moving the reading frame. (experiome.com)
  • Only three genes comprise the bacteriophage virus R17, while between five and ten genes comprise the SV40. (javatpoint.com)
  • On a single, 1 mm long chromosome, the E. coli bacteria have more than 3000 genes. (javatpoint.com)
  • The Pur alpha-Rbcomplexes contain a form of Pur alpha with extensive post-syntheticmodification, as demonstrated following expression of Pur alpha cDNA fusedto a 9-amino acid epitope tag. (embl-heidelberg.de)
  • Sequence of cDNA comprising the human pur gene and sequence-specificsingle-stranded-DNA-binding properties of the encoded protein. (embl-heidelberg.de)
  • PANDAA-quantified DRMs present at frequency ≥5% (2 h from nucleic acid to result) with a sensitivity and specificity of 96.9% and 97.5%, respectively. (nature.com)
  • The use of PCR and real-time PCR (qPCR)-based methodologies in clinical diagnostic virology demands high sensitivity and specificity for the selected viral nucleic acid target. (nature.com)
  • Analytical specificity comprises the extent of inclusivity by which all viral phylogenetic variants are captured at the exclusion of its genetic near neighbors. (nature.com)
  • It is to be borne in mind that chromosome in prokaryotes comprises only a single circular DNA. (biologywise.com)
  • Prokaryotes have evolved a nucleic acid-based immune system that shares some functional similarities with RNA interference in eukaryotes. (berkeley.edu)
  • It is the adaptive immune system in prokaryotes where small guide RNAs are employed for sequence-specific interference with invading nucleic acids. (experiome.com)
  • Structural studies of the Streptococcus pyogenes Cas9 showed that the protein exhibits a bilobed architecture comprising the catalytic nuclease lobe and the α-helical lobe of the enzyme and interactions between the two lobes seem to be mediated primarily through contacts with the bound nucleic acid rather than direct protein-protein contacts. (universityofcalifornia.edu)
  • The catalytic region comprises six conserved sequence motifs (I, III, IIIa, IV, V-VI), motif I includes the lysine residue that is adenylated in the first step of the ligation reaction. (embl.de)
  • In another embodiment, an isolated nucleic acid encoding the GEF-H1 peptide of SEQ ID NO:3 is provided. (justia.com)
  • In similar fashion, one other embodiment provides an isolated nucleic acid encoding the GEF-H1 peptide of SEQ ID NO:4. (justia.com)
  • Here, we present a 3D-printable kit for the construction of DNA and peptide nucleic acid (PNA). (allenpress.com)
  • Expression of the L1 protein using recombinant DNA technology produces noninfectious virus-like particles (VLPs). (cdc.gov)
  • The DNA damage repair gene XRCC1 (X-ray repair cross complementing gene 1) functions in base excision repair and the repair of single-strand breaks that are caused by ionizing radiation and oxidative damage [ 2 ]. (hindawi.com)
  • Through the DNA double-strand break(DSB) repair pathway, transposase-dependent DNA integration, base editing, and gene regulation using the CRISPR-dCas or type VI CRISPR system, CRISPR approaches can produce both quantitative and qualitative changes in gene expression. (experiome.com)
  • DNA methylation and histone modifications play a central role in the epigenetic regulation of gene expression and cell differentiation. (cipsm.de)
  • A gene is made up of two strands that are coiled around one another. (javatpoint.com)
  • Varying organisms have different total gene counts on a single chromosome. (javatpoint.com)
  • So the prokaryotic Argonaute is a nucleic acid guided endonuclease, which means unlike normal endonucleases where you'll have a cutting at a lot of random locations, prokaryotic Argonaute uses a short nucleic acid guide to bring its activity to a very specific sequence in a substrate that's complimentary to that guide. (neb.com)
  • And then each active site cuts each strand of that double-stranded substrate. (neb.com)
  • So in the case of a double stranded substrate, you'd just be using it like a nickase. (neb.com)
  • Argonautes from different prokaryotic species may utilize either DNA or RNA guides to target DNA or RNA substrates, leading to many potential combinations of guide and substrate preferences. (neb.com)
  • For example, the technology provides a semiconductor device with a substrate comprising a groove. (rpi.edu)
  • citation needed] The isolated enzyme has no intrinsic helicase activity but may carry out an equivalent function by way of its strong binding to single stranded DNA, particularly in preference to double stranded nucleic acid. (wikipedia.org)
  • The enzyme facilitates the "debranching" of double stranded DNA. (wikipedia.org)
  • It's a tiny, thermally controlled, cyclical, enzyme-driven, chemical reaction which lets scientists identify the presence of DNA both very specifically (you identify what you aim to, and not what you don't) and very sensitively (you can identify amounts too small to find otherwise). (virologydownunder.com)
  • DNA ligase (polydeoxyribonucleotide synthase) is the enzyme that joins two DNA fragments by catalysing the formation of an internucleotide ester bond between phosphate and deoxyribose. (embl.de)
  • Virions consist, of a central DNA/protein core surrounded in turn by an internal limiting membrane, a viral capsid, and, in the case of those particles that bud from the plasma membrane, an outer viral envelope (Figure 1. (ictv.global)
  • The virion core is electron-dense and consists of a DNA-protein filament enclosed within an icosahedral capsid (Figure 1. (ictv.global)
  • Both types of structure predominantly comprise hexavalent capsomers, a total of 1,460 per virion, that are composed of the major capsid protein (MCP). (ictv.global)
  • Pur-alpha can influence viral interaction through functional associations, for example with the Tat protein and TAR RNA of HIV-1, and with large T-antigen and DNA regulatory regions of JC virus. (embl-heidelberg.de)
  • Recent work has demonstrated the role of CRISPRs in adaptive immunity and shown that small RNAs derived from CRISPRs (crRNAs) are implemented as homing oligos for the targeted interference of foreign DNA. (berkeley.edu)
  • This molecule consists of two strands which wrap around each other, forming hydrogen bonds in the middle of the structure for support. (onelovefilm.com)
  • At the same time, the two circular DNA strands migrate and attach themselves to plasma membrane in different sites. (biologywise.com)
  • Vertebrate cells encode three well-characterised DNA ligases (DNA ligases I, III and IV), all of which are related in structure and sequence. (embl.de)
  • To protect it with a gap-free protein shell, 30 Å thick, would require roughly 25,000 amino acid residues-far more than the viral nucleic acid can encode. (basicmedicalkey.com)
  • Building on previous research, representative nucleic acid duplexes were split into logical monomer segments, and atomic coordinates were used to create solid models for 3D printing. (allenpress.com)
  • Dengue virus (DENV) is an enveloped, single-stranded RNA virus belonging to the Flaviviridae family. (frontiersin.org)
  • As subunits only comprise part of a virus, they can struggle to elicit a robust-enough immune response to confer adequate protection. (thenativeantigencompany.com)
  • It is an enveloped virus with two copies of single-stranded RNA, which have capacity to recombine. (medscape.com)
  • So depending on the species that the Argonaute is from, they can target DNA or RNA substrates and they can use DNA or RNA guides. (neb.com)
  • For example, Thermus thermophilus Argonaute is a DNA guided DNA endonuclease, but it also has some activity on RNA substrates as well. (neb.com)
  • A method based on atomic force microscopy is used to delineate the properties that determine single-molecule adhesion onto solid substrates in aqueous environment. (cipsm.de)
  • The stochastic nature of single-molecule charge transport measurements requires collection of large data sets to capture the full complexity of a molecular system. (imperial.ac.uk)
  • Here we present such an approach based on multivariate pattern analysis and apply it to simulated and experimental single-molecule charge transport data. (imperial.ac.uk)
  • These hypotheses will be tested through an integrated approach that incorporates biochemical, genetic, and single-molecule FRET techniques. (grantome.com)
  • SARS-CoV-2 is encoded by a positive-sense, single-stranded RNA molecule that can be mixed with host RNA during isolation from a sample. (qiagen.com)
  • While viral genomes can be comprised of RNA or DNA, SARS-CoV-2 is encoded by an RNA molecule. (qiagen.com)
  • When provided with synthetic nucleic acid guides, prokaryotic Argonautes can be used as programmable nucleases. (neb.com)
  • Chemical ASOs with synthetic synthetic fibers comprising twenty bases that can control genetic expression by binding to the " mental " string of mRNA targets. (newswingz.com)
  • Dynamic and flexible nucleic acid models can provide current and future scientists with physical intuition for the structure of DNA and the ways that DNA and its synthetic mimics can be used to build self-assembling structures and advanced nanomachines. (allenpress.com)
  • Resulting handheld helical models replicate the geometries in published structures and can now flex to form crossovers and allow biologically relevant zipping and unzipping to allow complex demonstrations of nanomachines undergoing strand displacement reactions. (allenpress.com)
  • Structural DNA nanotechnology is a versatile technique for the self-assembly of structures and dynamic machines with versatile chemical functionality. (allenpress.com)
  • This field first arose from the work of Nadrian Seeman, who recognized that DNA could form lattices and junctions to form ordered structures for the arrangement of material at the nanoscale ( 1 ). (allenpress.com)
  • The shape, mechanics, and thermodynamics of these structures result from their complex internal architecture, and given the extraordinary utility of structural DNA nanotechnology for advancing the field of biophysics, educational tools are needed for teaching key structural concepts fundamental to the design of DNA-based nanostructures. (allenpress.com)
  • Their binding properties depend on the amino acid sequence of the finger domains and of the linker between fingers, as well as on the higher-order structures and the number of fingers. (embl.de)
  • The structures of many DNA quadru- magnetization ( 11 - 13 ). (lu.se)
  • The first aim i s to dissect the nucleic acid-binding mechanisms of the Est3-TEN complex (comprised of two critical and conserved domains in the telomerase holoenzyme) and assess their contribution to telomerase activity and processivity in vitro and in vivo. (grantome.com)
  • Thus, in one embodiment, peptides comprising the sequences described in SEQ ID NOs. (justia.com)
  • CRISPR-harboring organisms generate immunological memory of previous infections by capturing short segments of foreign DNA for integration into CRISPR loci as spacer sequences. (berkeley.edu)
  • Cas9 contains two nuclease active sites that function together to generate DNA double-strand breaks (DSBs) at sites complementary to the guide RNA sequence and adjacent to a protospacer adjacent motif. (universityofcalifornia.edu)
  • They cause double-stranded breaks (DSBs), which can be repaired using one of two DNA repair pathways: homology directed repair (HDR) or non-homologous end joining (NHEJ). (experiome.com)
  • where X can be any amino acid, and number indicates the number of residues. (embl.de)
  • Our results uncover the structural basis for foreign DNA capture and the mechanism by which Cas1-Cas2 functions as a molecular ruler to dictate the sequence architecture of CRISPR loci. (berkeley.edu)
  • As more research labs and classrooms dive into the field of structural nucleic acid nanotechnology, students and researchers need access to interactive, dynamic, handheld models. (allenpress.com)
  • High-bandwidth detection of short DNA in nanopipettes. (imperial.ac.uk)
  • Recently, we have demonstrated high-bandwidth detection of double-stranded (ds) DNA with microsecond time resolution in nanopipettes, using custom-designed electronics. (imperial.ac.uk)
  • The detection of the DNA PCR products moved PCR from its conventional form to its real-time (rPCR) form. (virologydownunder.com)