Nucleic acid base sequence. Medical search. Frequent questions

Methods A next-generation sequencing (NGS) panel was created for the human TRPV1 gene and in addition, for the leukotriene receptors BLT1 and BLT2 recently described to modulate TRPV1 mediated sensitisation processes rendering the coding genes LTB4R and LTB4R2 important co-players in pharmacogenetic approaches involving TRPV1. (researchgate.net)
MERS-CoV) via next generation sequencing (NGS) from found in Wuhan, but it would appear samples prepared for cultured virus or directly from samples received from molecular testing could be handled as would samples of several pneumonia patients. (who.int)
The Public Health Agency's Next Generation Sequencing (NGS) platform performs massive parallel sequencing of DNA and RNA. (folkhalsomyndigheten.se)
Center for Translational Genomics (CTG) is a research infrastructure and technical platform for Next Generation Sequencing (NGS) and other genomics technologies at the Medical Faculty of Lund University. (lu.se)

http://ijgvd.megabank.tohoku.ac.jp/ ) provides genomic variation data detected by whole-genome sequencing (WGS) of Japanese individuals. (nature.com)
Major NAT technologies include polymerase chain reaction (PCR) used for specificity, strand displacement amplification (SDA), ligase chain reaction (LCR), transcription-mediated amplification (TMA) nucleic acid sequence based amplification (NASBA) and whole genome sequencing. (prsync.com)
For contact tracing or inquiries related to antibiotic resistance, whole genome sequencing (WGS) of bacterial isolates is offered. (folkhalsomyndigheten.se)
Whole genome sequencing can also provide insights into the specific genes or mutations that may be linked to antibiotic resistance. (folkhalsomyndigheten.se)
It is a platform and database for the automated analysis of whole genome sequencing data. (folkhalsomyndigheten.se)
Our DNA services includes whole genome, whole exome or targeted library preparation and sequencing on Illumina platforms. (lu.se)

DNA Electronics develops scalable semiconductor technology for real-time nucleic acid detection, "which enables faster, simpler, and more cost-effective DNA analysis platforms," it said in a statement. (genomeweb.com)

Incorporating Locked Nucleic Acid into oligonucleotides increases thermal duplex stability 2 and improves the specificity of oligonucleotide hybridization to target sequences. (sigmaaldrich.com)
In addition, the improved hybridization of the PCR primer or qPCR probe to its target may increase the melting temperature (T m ) by up to 8 °C per Locked Nucleic Acid monomer substitution in medium salt conditions compared to native-state DNA oligonucleotides 4 ( Table 1 ). (sigmaaldrich.com)
Due to Locked Nucleic Acid's improved hybridization characteristics with accompanied increase in T m , Locked Nucleic Acid oligonucleotides can be synthesized to be shorter, which overcomes certain design limitations that occur with native-state DNA oligonucleotides. (sigmaaldrich.com)
Agilent offers other hybridization-based target enrichment technology options, also, for finding specific regions-of-interest. (agilent.com)
The microarray technology is based on the hybridization of labelled DNA or RNA molecules to complementary sequences that are immobilized on a solid substrate. (tutorialspoint.com)
The principle of DNA microarray technology is based on the hybridization of complementary sequences of nucleic acid molecules. (tutorialspoint.com)
The hybridization occurs between the complementary sequences of the probes and the target molecules. (tutorialspoint.com)
The principle of DNA microarray is based on the hybridization of DNA strands. (tutorialspoint.com)
in vitro, they are formed by nucleic acid hybridization. (umassmed.edu)
Peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) assay for specific detection of mycobacterium immunogenum and DNA-FISH assay for analysis of pseudomonads in metalworking fluid s and sputum. (cdc.gov)
Here, we report for the first time a species-specific peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) method for Mycobacterium immunogenum, a non-tuberculous Mycobacterium species prevalent in MWF and implicated in occupational lung disease hypersensitivity pneumonitis and pseudo-outbreaks. (cdc.gov)
During the hybridization step, the sequence specific regions of the capture oligomers bind to specific regions of the target molecules. (cdc.gov)
Detection of the rRNA amplification product sequences is achieved using nucleic acid hybridization (HPA). (cdc.gov)

Each group of three bases, called a codon, corresponds to a single amino acid, and there is a specific genetic code by which each possible combination of three bases corresponds to a specific amino acid. (wikipedia.org)
Its polymerases provide a wide range of applications for DNA amplification, repair, sequencing, detection, and modification of genetic targets of clinical value. (globaldata.com)
Registration of 252 sequenced sorghum mutants as a community reverse genetic resource. (usda.gov)
Multigene phylogenies constructed from multiplexed and Sanger sequencing data are regularly used in mycology and other disciplines as a cost-effective way of species identification and as a first means to investigate genetic diversity samples. (apsnet.org)
This study is the first report on identifying bolting and flowering-related lncRNAs based on transcriptome sequencing in spinach, which provides a useful resource for future functional genomics studies, genes expression researches, evaluating genes regulatory networks and molecular breeding programs in the regulation of the genetic mechanisms related to bolting in spinach. (biomedcentral.com)
Xanthine is not part of the genetic code, but it is found in living organisms from the breakdown of guanine, which is a base in DNA and RNA. (creation.com)
Sequence-directed genetic interference pathways control gene expression and preserve genome integrity in all kingdoms of life. (rockefeller.edu)
Metagenomic sequencing engables a thorough mapping of the genetic material in the clinical sample, providing a broader understanding of the microbiological profile. (folkhalsomyndigheten.se)
In contrast to metagenomic detection of unknown pathogens which sequences all available genetic material, 16S sequencing specifically targets the small ribosomal subunit. (folkhalsomyndigheten.se)
By sequencing the entire genome, a comprehensive understanding of the genetic composition is obtained. (folkhalsomyndigheten.se)
In epidemiological contact tracing, this technology enables the mapping of infection spread and identification of sources of infection through the comparison of genetic sequences. (folkhalsomyndigheten.se)

The sequence of nucleobases on a nucleic acid strand is translated by cell machinery into a sequence of amino acids making up a protein strand. (wikipedia.org)
Primers were designed to target sequences in the nodavirus capsid protein gene, yielding an amplification product of 120 nucleotides. (int-res.com)
CRISPR/Cas9-based genome-wide screening of the deubiquitinase subfamily identifies USP3 as a protein stabilizer of REST blocking neuronal differentiation and promotes neuroblastoma tumorigenesis. (nih.gov)
PASS2 version 6: a database of structure-based sequence alignments of protein domain superfamilies in accordance with SCOPe. (ncbs.res.in)
COBALT computes a multiple protein sequence alignment using conserved domain and local sequence similarity information. (nih.gov)
COBALT is a multiple sequence alignment tool that finds a collection of pairwise constraints derived from conserved domain database, protein motif database, and sequence similarity, using RPS-BLAST, BLASTP, and PHI-BLAST. (nih.gov)
The tests used for protein C testing can include enzyme-linked immunosorbent assay (ELISA), chromogenic assays, and the activated partial thromboplastin time (aPTT)-based functional protein C assay. (medscape.com)
A thrombin-generation-based test has also been shown to detect protein C deficiency. (medscape.com)
The aPTT-based functional protein C assay may yield misleadingly low protein C levels in the presence of (1) a factor V Leiden mutation and some other causes of activated protein C resistance, (2) elevated plasma factor VIII levels, or (3) hyperlipidemia. (medscape.com)

A cohort of 80 healthy subjects of Western European descent was screened to evaluate and validate the detection of exomic sequences of the coding genes with 25 base pair exon padding. (researchgate.net)
Conclusions Results suggested that the NGS approach based on AmpliSeq™ libraries and Ion Personal Genome Machine (PGM) sequencing is a highly efficient mutation detection method. (researchgate.net)
Based on the detection of cell culture-derived nodavirus, and a synthetic RNA target, the real-time NASBA procedure was approximately 100-fold more sensitive than single-tube RT-PCR. (int-res.com)
Three major processes involved in NAT are sample pooling and nucleic acid (NA) extraction, target NA amplification and target amplicon NA detection. (prsync.com)
Locked Nucleic Acid PCR primers and qPCR probes are compatible with all real-time thermocyclers and end-point analytical detection instruments. (sigmaaldrich.com)
However, one of the challenges in the detection of low-frequency viral strains concerns the errors introduced during the sequencing process. (biomedcentral.com)
laboratories that can perform pan coronavirus detection and A single negative test result, particularly if this is from an directed sequencing. (who.int)
In 2001, they obtained the 540 patent, which does not claim cffDNA or paternally inherited cffDNA, but claims methods of using cffDNA to diagnose certain fetal characteristics based on the detection of paternally inherited cffDNA. (justia.com)
Currently, there are three basic types of tests to determine if an individual has been infected with SARS-CoV-2: viral nucleic acid (RNA) detection, viral antigen detection, and detection of antibodies to the virus. (medscape.com)
Viral tests (nucleic acid or antigen detection tests) are used to assess acute infection, whereas antibody tests provide evidence of prior infection with SARS-CoV-2. (medscape.com)
Wooden shafts or calcium alginate swabs may contain substances that inhibit some viruses and nucleic acid detection reactions. (medscape.com)

ABSTRACT: Nucleic acid sequence based amplification (NASBA) is an isothermal nucleic acid amplification procedure based on target-specific primers and probes, and the co-ordinated activity of 3 enzymes: AMV reverse transcriptase, RNase H, and T7 RNA polymerase. (int-res.com)

Electron microscopic analysis of the resulting heteroduplexes facilitates the mapping of regions of base sequence homology of nucleic acids. (umassmed.edu)

A nucleic acid sequence is a succession of bases within the nucleotides forming alleles within a DNA (using GACT) or RNA (GACU) molecule. (wikipedia.org)
Nucleic acids consist of a chain of linked units called nucleotides. (wikipedia.org)
Calculate the percent difference by taking the number of differences between the DNA bases divided by the total number of nucleotides. (wikipedia.org)
This identified approximately 140 chromosome loci where nucleotides deviated from the reference sequence GRCh37 hg19 comprising the three genes TRPV1, LTB4R and LTB4R2. (researchgate.net)
Every organism demonstrates different DNA sequences with specific nucleotides. (mdpi.com)

The Nucleic Acid Testing is also known as NAT (Nucleic Acid Test) or NAAT (Nucleic Acid Amplification Test). (prsync.com)
The nucleic acid amplification test (NAAT) involves the amplification of specific nucleic acid sequences of the SARS-CoV-2 virus by targeting specific nucleic acid sequences. (globaldata.com)

Since nucleic acids can bind to molecules with complementary sequences, there is a distinction between "sense" sequences which code for proteins, and the complementary "antisense" sequence, which is by itself nonfunctional, but can bind to the sense strand. (wikipedia.org)
Nucleic acid cytometry using droplet microfluidics identifies and sorts single cells, virus, or free molecules based on specific "keyword" sequences. (rsc.org)
The probes are complementary to specific sequences of DNA or RNA molecules that are to be analysed. (tutorialspoint.com)
Double-stranded nucleic acid molecules (DNA-DNA or DNA-RNA) which contain regions of nucleotide mismatches (non-complementary). (umassmed.edu)
The capture oligomers contain sequences complementary to specific regions of the target molecules as well as a string of deoxyadenosine residues. (cdc.gov)

Peptide nucleic acid (PNA) amphiphiles: synthesis, self-assembly, and duplex stability. (umassmed.edu)

A novel species-specific 14-bp PNA probe was designed for M. immunogenum based on its 16S rRNA gene sequence and was validated for specificity, by testing against a panel of other phylogenetically closely related rapidly growing mycobacteria and representative species of gram-positive, gram-negative, and acid fast organisms. (cdc.gov)

High-throughput RNA sequencing (RNA-seq) and bioinformatics were used to identify the potential microorganisms. (asm.org)

In biological systems, nucleic acids contain information which is used by a living cell to construct specific proteins. (wikipedia.org)
The central dogma of molecular biology outlines the mechanism by which proteins are constructed using information contained in nucleic acids. (wikipedia.org)
The discipline of biophotonics deals with the interaction of light, or electromagnetic radiation, with living organisms and biologically active macromolecules: proteins (hemoglobin), nucleic acids (DNA and RNA), and metabolites (glucose and lactose). (nationalacademies.org)
RStrucFam: a web server to associate structure and cognate RNA for RNA-binding proteins from sequence information. (ncbs.res.in)
One of the first discoveries was amino acids, the components of proteins. (creation.com)

The NGS workflow was based on a custom AmpliSeq™ panel and designed for sequencing of human genes on an Ion PGM™ Sequencer. (researchgate.net)
It is suitable for large-scale sequencing of TRPV1 and functionally related genes. (researchgate.net)
In a Novartis-sponsored study in the New England Journal of Medicine, researchers found that a CRISPR-Cas9-based treatment targeting promoters of genes encoding fetal hemoglobin could reduce disease symptoms. (genomeweb.com)
Multigene phylogenies obtained through Sanger sequencing often use barcode sequences, specific genic or intergenic fragments of well-defined genes, that have been widely used over the past decades. (apsnet.org)
2015. Genome sequencing of herb Tulsi (Ocimum tenuiflorum) unravels key genes behind its strong medicinal properties. . (ncbs.res.in)
Results from RNA sequencing revealed that AEG‑1 modulated neuronal morphology and development by regulating the expression of numerous genes, such as ubiquitin C, C‑X‑C motif chemokine ligand 1, MMP9, Notch1, neuropilin 1 and ephrin type‑A receptor 4. (spandidos-publications.com)
We developed TS-REX, a database/software system that supports the analysis of tissue and cell type-specific transcription factor-gene networks based on expressed sequence tag abundance of transcription factor-encoding genes in UniGene EST libraries. (lu.se)

The laboratory procedure is based on amplifying and sequencing four variable regions of the 16S rRNA gene with two amplicons. (folkhalsomyndigheten.se)

We present AB12PHYLO, an integrated pipeline that can perform all necessary steps from reading raw Sanger sequencing data through visualizing and editing phylogenies. (apsnet.org)
RNA Sanger sequencing analysis was performed in AEG‑1‑deficient HT22 cells and wild‑type HT22 cells without knockout (KO). (spandidos-publications.com)

Example of comparing and determining the % difference between two nucleotide sequences AATCCGCTAG AAACCCTTAG Given the two 10-nucleotide sequences, line them up and compare the differences between them. (wikipedia.org)

With regards to transcription, a sequence is on the coding strand if it has the same order as the transcribed RNA. (wikipedia.org)
The prediction of transcription factor binding sites in genomic sequences is in principle very useful to identify upstream regulatory factors. (lu.se)

Target amplification assays are based on the ability of complementary oligonucleotide primers to specifically anneal and allow enzymatic amplification of the target nucleic acid strands. (cdc.gov)

Nucleic Acid Heteroduplexes" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (umassmed.edu)
This graph shows the total number of publications written about "Nucleic Acid Heteroduplexes" by people in this website by year, and whether "Nucleic Acid Heteroduplexes" was a major or minor topic of these publications. (umassmed.edu)
Below are the most recent publications written about "Nucleic Acid Heteroduplexes" by people in Profiles. (umassmed.edu)

Deep-sequencing allows for an in-depth characterization of sequence variation in complex populations. (biomedcentral.com)
Modern massive parallel sequencing (MPS) technologies, on the contrary, have the opportunity to allow in-depth characterization of sequence variation in more complex populations, including low-frequency viral strains. (biomedcentral.com)

Locked Nucleic Acid in PCR primers, qPCR probes, and other types of oligonucleotides is soluble in water and standard buffers as well as follows Watson-Crick base-pairing rules. (sigmaaldrich.com)

Biological deoxyribonucleic acid represents the information which directs the functions of an organism. (wikipedia.org)
Nucleic Acid-Based Identification Methods for Infectious Disease Nucleic acid-based methods detect organism-specific DNA or RNA sequences extracted from the microorganism. (msdmanuals.com)

Cultures or species-specific genus-specific real-time PCR in combination with amplicon real-time PCR for M. avium and M. tuberculosis remained sequencing and a M. haemophilum -specific PCR resulted negative. (cdc.gov)
For example, AT-rich native-state DNA oligonucleotides often need to be over 30 bases long (sometimes over 40 bases) to satisfy amplicon design guidelines but may still perform poorly. (sigmaaldrich.com)
Agilent׷ HaloPlex / HaloPlex HS amplicon-based technology for target enrichment and amplification offers an NGS solution with speed, accuracy, and sensitivity. (agilent.com)
HaloPlex HS is a high-sensitivity, amplicon-based targeted sequencing method that incorporates molecular barcodes in the DNA library. (agilent.com)

1: Chatterjee A, Stockwell P A, Rodger E J, and Morison I M, Comparison of alignment software for genome-wide bisulphite sequence data , Nucleic Acids Research Volume 40, Issue 10Pp. (otago.ac.nz)

Nucleic acids are the basic units of deoxyribonucleic acid (DNA) sequencing. (mdpi.com)

Sequences may or may not be amplified in vitro. (msdmanuals.com)

Using terial infection was diagnosed in these patients because polymerase chain reaction (PCR) technology on fine nee- either acid-fast staining results were positive or the dle aspirates and biopsied specimens from 89 children with Mycobacterium genus-specific real-time polymerase chain cervicofacial lymphadenitis, we assessed the importance of M. haemophilum . (cdc.gov)
Biopsied specimens are subjected to acid-fast er (ITS) sequencing. (cdc.gov)
Additionally, a BLAST search in a reference database is often performed to identify sequences of type specimens to compare the samples within the phylogeny. (apsnet.org)

The COVID-19 nucleic acid amplification tests pipeline product market report provides comprehensive information about the pipeline products with a comparative analysis of the products at various stages of development and information about the clinical trials which are in progress. (globaldata.com)
Diagnosis of rabies based on clinical symptoms alone is difficult and unreliable, except when there are specific clinical signs of hydrophobia or aerophobia [3]. (scielo.br)
All nucleic acid in the clinical sample is sequenced and the resulting data is analysed by experienced bioinformaticians. (folkhalsomyndigheten.se)
The result of of metagenomic sequencing is the outcome of a collaborative effort between bioinformaticians and clinical microbiologists, where the clinical interpretation confirms and complements the technical analysis. (folkhalsomyndigheten.se)
16S sequencing is performed to determine which bacterial species are present in a clinical sample or isolate. (folkhalsomyndigheten.se)
The basis of the GENSAM project is the joint national management of clinical microbial sequencing data. (folkhalsomyndigheten.se)

The nucleobases are important in base pairing of strands to form higher-level secondary and tertiary structures such as the famed double helix. (wikipedia.org)

Table 1 Increasing the number of Locked Nucleic Acid bases in an oligonucleotide increases the T m . (sigmaaldrich.com)
T m of duplex between an oligonucleotide and its complementary sequence. (sigmaaldrich.com)
The presence of a single base mismatch has a greater destabilizing effect on the duplex formation between an Locked Nucleic Acid oligonucleotide and its target than with a native-state DNA oligonucleotide. (sigmaaldrich.com)
The cDNA microarray uses complementary DNA as probes, while the oligonucleotide microarray uses short DNA sequences as probes. (tutorialspoint.com)

In this project, the genomic DNA of 1,070 subjects obtained from peripheral blood samples was subjected to paired-end sequencing using the Illumina HiSeq 2500 platform. (nature.com)
Fortunately, base calls are complemented with quality scores which are derived from a quadruplet of intensities, one channel for each nucleotide type for Illumina sequencing. (biomedcentral.com)
Moreover, the incorporation of quality scores is most appropriate for Illumina sequencing data. (biomedcentral.com)

Specifically, Locked Nucleic Acid oligonucleotides can be designed to address traditionally problematic target sequences, such as AT- or GC-rich regions. (sigmaaldrich.com)

Locked Nucleic Acid Dual-Labeled Probes discriminate better than DNA Dual-Labeled Probes in SNP genotyping analysis9. (sigmaaldrich.com)

Pink) mutant DNA analysis with Locked Nucleic Acid mutant probe (16mer with 3 Locked Nucleic Acid bases). (sigmaaldrich.com)
Purple) wild-type DNA with Locked Nucleic Acid mutant Probe (16mer with 3 Locked Nucleic Acid bases). (sigmaaldrich.com)

Nucleic acid mapping to Plasmodium was also used on the patient samples, confirming results obtained with an antigen-based rapid diagnostic test (RDT) conducted in the field laboratories. (asm.org)

The ability of oligonucleotides to discriminate between alleles via SNPs is greatly enhanced by the incorporation of Locked Nucleic Acid bases 6-8 ( Figure 2 ). (sigmaaldrich.com)

13 The sequence reads were mapped onto the human reference genome, assembly GRCh37/hg19, with decoy sequences (hs37d5) and an average sequencing coverage of 32.4× for full-length autosomal chromosomes. (nature.com)

Nucleic Acid Testing has been widely used for Viral and Bacterial Infectious Diseases, Cancerous diseases, Forensic Testing, Genetically related Diseases and Paternity Testing/HLA Typing. (prsync.com)
A virus variant model-based clustering method, ViVaMBC, is presented that explores quality scores and second best base calls for identifying and quantifying viral variants. (biomedcentral.com)
Le VRS demeure un agent étiologique viral important au Maroc, responsable de syndromes de type grippal et d'infections respiratoires aiguës sévères, en particulier chez les nourrissons. (who.int)

Nous avons réalisé une étude prospective en recourant à la surveillance de la grippe basée sur des sites sentinelles dans le but de dépister le VRS grâce à la PCR en temps réel chez des patients souffrant d'infections respiratoires aiguës recrutés au cours de deux saisons (2014-2015 et 2015-2016). (who.int)

This distance overestimates exponentially scaled percentage of different residues in aligned sequences (see graphs in the above paper for details). (nih.gov)

This option can be unchecked for aligning of sequences that are not expected to share conserved domains and are expected to have very short pair wise local alignments. (nih.gov)
Notably, we now provide family alignments based on four different representative proteome sequence data sets and a new interactive DNA search interface. (bvsalud.org)

If one strand of the double-stranded DNA is considered the sense strand, then the other strand, considered the antisense strand, will have the complementary sequence to the sense strand. (wikipedia.org)

See Edgar RC, Nucleic Acids Res 16:380-5, 2004, PMID: 14729922 for k-mer counting-based sequence similarity. (nih.gov)
The distance between two sequences is computed as a fraction of words that appear in both sequences with respect to number of all words in the longer sequence (similarly as in Edgar RC, Nucleic Acids Res 16:380-5, 2004, PMID: 14729922 ). (nih.gov)

Click molecule labels to explore molecular sequence information. (nih.gov)
Nucleic acid-based (molecular) identification. (msdmanuals.com)

Previously, an attempt to characterize these in the recognition of M. haemophilum as the causative mycobacteria by sequence analysis of the genus-specific agent in 16 (18%) children with cervicofacial lymphadenitis. (cdc.gov)
With Locked Nucleic Acid oligonucleotides, the selective placement of Locked Nucleic Acid bases facilitates the optimal design of highly-specific, shorter oligonucleotides that perform well, even at lengths of only 13 to 20 bases. (sigmaaldrich.com)
This paper proposes a generative adversarial networks (GAN) model to create synthetic nucleic acid sequences of the cat genome tuned to exhibit specific desired properties. (mdpi.com)

Aoki T, Tahara T, Satoh K, Fujino H, Watabe H (2003) General properties of GFP-display, an electrophoretic analysis for single amino acid changes in target polypeptides. (springer.com)
AEG‑1 is associated with glutamate excitotoxicity due to its reported function of repressing excitatory amino acid transporter 2 expression in astrocytes. (spandidos-publications.com)
In AST, AEG-1 causes glutamatergic excitotoxicity by downregulating the activity of the excitatory amino acid transporter 2 (EAAT2) promoter, leading to neuronal cell death in glioma-induced neurodegenerative disease ( 10 ). (spandidos-publications.com)
1 Later, there were dubious claims that some of the amino acids had a slight excess of the 'handedness' ( chirality ) required for life, as we have reported . (creation.com)
For example, Sandra Pizzarello, a chemist at Arizona State University in Tempe, US, who has previously researched amino acids in this meteorite, is unconvinced. (creation.com)
Cannon W, Gallegos MT, Casaz P, Buck M. Amino-terminal sequences of sigmaN (sigma54) inhibit RNA polymerase isomerization. (umassmed.edu)

Spacer sequences are transcribed into CRISPR RNAs (crRNAs)-small RNAs that, through base-pairing interactions with the target sequence, guide Cas nucleases to the invasive nucleic acid to cleave it. (rockefeller.edu)

DNA ExtractionWe recommend Qiagen spin column-based nucleic acids purification methods. (lu.se)

However there is no parallel concept of secondary or tertiary sequence. (wikipedia.org)
We describe isogenic colony sequencing (ICO-seq), a massively-parallel strategy to assess the gene expression profiles of large numbers of genetically distinct yeast colonies. (rsc.org)

They provided a slight increase in sensitivity, which however does not warrant the additional computational cost of running the offline base caller. (biomedcentral.com)

To make a reference panel of genomic variation for the Japanese population, we sequenced whole genomes of 1,070 cohort participants, and detected genomic variants including SNVs, indels and structural variants. (nature.com)
Within CRISPR sites, the repeats are separated by short spacer sequences that match phage or plasmid genomes and specify the targets of interference. (rockefeller.edu)

Today, a number of tools exist for each of the steps for multigene phylogenetic analyses, including quality control and trimming, the generation of a multiple sequence alignment (MSA), extraction of informative sites, and the construction of the final phylogenetic tree. (apsnet.org)
Genomic Analysis and Comparative Multiple Sequence of SARS-CoV2. (cdc.gov)

On the basis of geography, Global Nucleic Acid Testing Market is segmented into seven key regions: North America, South America, Eastern Europe, Western Europe, Asia Pacific, Japan, and Middle East & Africa. (prsync.com)

Nucleic acid sequencing expresses the evolutionary changes among organisms and revolutionizes disease diagnosis in animals. (mdpi.com)

All sequencing libraries were constructed based on PCR-free methods. (nature.com)
Standard genotyping such as direct PCR sequencing methods, however, only provides information on the most abundant sequence variants. (biomedcentral.com)
Metagenomic sequencing is offered in cases where infection is suspected but other methods have failed to identify a pathogen. (folkhalsomyndigheten.se)

Apparently a lot of information is already contained in the quality scores enabling the model based clustering procedure to adjust the majority of the sequencing errors. (biomedcentral.com)

Anatomy4

Organisms9

Diseases7

Chemicals and Drugs120

Analytical, Diagnostic and Therapeutic Techniques and Equipment40

Phenomena and Processes70

Disciplines and Occupations5

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture5

Information Science12

Health Care4

Next Generation Sequencing4

Whole-genome6

Real-time nucleic acid1

Hybridization13

Genetic11

Protein9

Detection11

NASBA1

Homology1

Nucleotides5

NAAT2

Molecules5

Peptide1

Specificity1

Bioinformatics1

Proteins5

Genes7

RRNA gene1

Sanger2

Nucleotide sequences1

Transcription2

Assays1

Heteroduplexes3

Characterization2

Primers1

Organism2

Amplicon4

Genome-wide1

Deoxyribonucleic1

Vitro1

Specimens3

Clinical6

Strands1

Oligonucleotide4

Illumina3

Target sequences1

Probes1

Probe2

Antigen1

Incorporation1

Decoy1

Viral3

20161

Residues1

Alignments2

Antisense1

PMID2

Molecular2

Specific3

Amino6

Interactions1

Purification1

Parallel2

Sensitivity1

Genomes2

Multiple sequence2

Regions1

Evolutionary1

Methods3

Procedure1