Nucleic acid base base sequence. Medical search. Frequent questions

http://ijgvd.megabank.tohoku.ac.jp/ ) provides genomic variation data detected by whole-genome sequencing (WGS) of Japanese individuals. (nature.com)
Major NAT technologies include polymerase chain reaction (PCR) used for specificity, strand displacement amplification (SDA), ligase chain reaction (LCR), transcription-mediated amplification (TMA) nucleic acid sequence based amplification (NASBA) and whole genome sequencing. (prsync.com)
Isolation and Whole-Genome Sequencing of Environmental Campylobacter. (cdc.gov)
Purification of Cyclospora cayetanensis oocysts obtained from human stool specimens for whole genome sequencing. (cdc.gov)
Integrating standardized whole genome sequence analysis with a global Mycobacterium tuberculosis antibiotic resistance knowledgebase. (cdc.gov)
For contact tracing or inquiries related to antibiotic resistance, whole genome sequencing (WGS) of bacterial isolates is offered. (folkhalsomyndigheten.se)
Whole genome sequencing can also provide insights into the specific genes or mutations that may be linked to antibiotic resistance. (folkhalsomyndigheten.se)
It is a platform and database for the automated analysis of whole genome sequencing data. (folkhalsomyndigheten.se)

Locked Nucleic Acid PCR primers and qPCR probes are compatible with all real-time thermocyclers and end-point analytical detection instruments. (sigmaaldrich.com)
A cohort of 80 healthy subjects of Western European descent was screened to evaluate and validate the detection of exomic sequences of the coding genes with 25 base pair exon padding. (researchgate.net)
Conclusions Results suggested that the NGS approach based on AmpliSeq™ libraries and Ion Personal Genome Machine (PGM) sequencing is a highly efficient mutation detection method. (researchgate.net)
Three major processes involved in NAT are sample pooling and nucleic acid (NA) extraction, target NA amplification and target amplicon NA detection. (prsync.com)
Its polymerases provide a wide range of applications for DNA amplification, repair, sequencing, detection, and modification of genetic targets of clinical value. (globaldata.com)
However, one of the challenges in the detection of low-frequency viral strains concerns the errors introduced during the sequencing process. (biomedcentral.com)
Currently, there are three basic types of tests to determine if an individual has been infected with SARS-CoV-2: viral nucleic acid (RNA) detection, viral antigen detection, and detection of antibodies to the virus. (medscape.com)
Viral tests (nucleic acid or antigen detection tests) are used to assess acute infection, whereas antibody tests provide evidence of prior infection with SARS-CoV-2. (medscape.com)
Wooden shafts or calcium alginate swabs may contain substances that inhibit some viruses and nucleic acid detection reactions. (medscape.com)
Detection of the rRNA amplification product sequences is achieved using nucleic acid hybridization (HPA). (cdc.gov)
laboratories that can perform pan coronavirus detection and A single negative test result, particularly if this is from an directed sequencing. (who.int)
1997) Detection of single-base changes using a bioluminometric primer extension assay. (jenabioscience.com)
Peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) assay for specific detection of mycobacterium immunogenum and DNA-FISH assay for analysis of pseudomonads in metalworking fluid s and sputum. (cdc.gov)
Clinical Metagenomic Next-Generation Sequencing for Pathogen Detection. (cdc.gov)
In contrast to metagenomic detection of unknown pathogens which sequences all available genetic material, 16S sequencing specifically targets the small ribosomal subunit. (folkhalsomyndigheten.se)

A method is described for visualizing three nucleic acid sequences simultaneously by in situ hybridization using a new blue immunofluorescent label, amino methyl coumarin acetic acid (AMCA), in combination with green and red fluorescing FITC and TRITC. (nih.gov)
Hybridization is the process of complementary base pairs binding to form a double helix. (wikipedia.org)
Incorporating Locked Nucleic Acid into oligonucleotides increases thermal duplex stability 2 and improves the specificity of oligonucleotide hybridization to target sequences. (sigmaaldrich.com)
In addition, the improved hybridization of the PCR primer or qPCR probe to its target may increase the melting temperature (T m ) by up to 8 °C per Locked Nucleic Acid monomer substitution in medium salt conditions compared to native-state DNA oligonucleotides 4 ( Table 1 ). (sigmaaldrich.com)
Due to Locked Nucleic Acid's improved hybridization characteristics with accompanied increase in T m , Locked Nucleic Acid oligonucleotides can be synthesized to be shorter, which overcomes certain design limitations that occur with native-state DNA oligonucleotides. (sigmaaldrich.com)
During the hybridization step, the sequence specific regions of the capture oligomers bind to specific regions of the target molecules. (cdc.gov)
Here, we report for the first time a species-specific peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) method for Mycobacterium immunogenum, a non-tuberculous Mycobacterium species prevalent in MWF and implicated in occupational lung disease hypersensitivity pneumonitis and pseudo-outbreaks. (cdc.gov)

Nucleic Acid Testing has been widely used for Viral and Bacterial Infectious Diseases, Cancerous diseases, Forensic Testing, Genetically related Diseases and Paternity Testing/HLA Typing. (prsync.com)
A virus variant model-based clustering method, ViVaMBC, is presented that explores quality scores and second best base calls for identifying and quantifying viral variants. (biomedcentral.com)
Modern massive parallel sequencing (MPS) technologies, on the contrary, have the opportunity to allow in-depth characterization of sequence variation in more complex populations, including low-frequency viral strains. (biomedcentral.com)
Isolation of cfDNA and viral nucleic acid is challenging because of its low abundance in typical samples. (thermofisher.com)
Le VRS demeure un agent étiologique viral important au Maroc, responsable de syndromes de type grippal et d'infections respiratoires aiguës sévères, en particulier chez les nourrissons. (who.int)
Metagenomic analysis of viral nucleic acid extraction methods in respiratory clinical samples. (cdc.gov)

High-throughput RNA sequencing (RNA-seq) and bioinformatics were used to identify the potential microorganisms. (asm.org)
With the ongoing developments of high throughput sequencing machines and advancement of modern bioinformatics tools at unprecedented pace, the target goal of sequencing individual genomes of living organism at a cost of $1,000 each is seemed to be realistically feasible in the near future. (springer.com)

The Nucleic Acid Testing is also known as NAT (Nucleic Acid Test) or NAAT (Nucleic Acid Amplification Test). (prsync.com)
The nucleic acid amplification test (NAAT) involves the amplification of specific nucleic acid sequences of the SARS-CoV-2 virus by targeting specific nucleic acid sequences. (globaldata.com)

Threats to timely sharing of pathogen sequence data. (cdc.gov)

The high-throughput - next generation sequencing (HT-NGS) technologies are currently the hottest topic in the field of human and animals genomics researches, which can produce over 100 times more data compared to the most sophisticated capillary sequencers based on the Sanger method. (springer.com)
Multigene phylogenies constructed from multiplexed and Sanger sequencing data are regularly used in mycology and other disciplines as a cost-effective way of species identification and as a first means to investigate genetic diversity samples. (apsnet.org)
We present AB12PHYLO, an integrated pipeline that can perform all necessary steps from reading raw Sanger sequencing data through visualizing and editing phylogenies. (apsnet.org)
Multigene phylogenies obtained through Sanger sequencing often use barcode sequences, specific genic or intergenic fragments of well-defined genes, that have been widely used over the past decades. (apsnet.org)
RNA Sanger sequencing analysis was performed in AEG‑1‑deficient HT22 cells and wild‑type HT22 cells without knockout (KO). (spandidos-publications.com)

Victors: a web-based knowledge base of virulence factors in human and animal pathogens. (cdc.gov)

The nucleic acid double helix is a spiral polymer, usually right-handed, containing two nucleotide strands which base pair together. (wikipedia.org)
Fortunately, base calls are complemented with quality scores which are derived from a quadruplet of intensities, one channel for each nucleotide type for Illumina sequencing. (biomedcentral.com)
We developed number of models using various types of features and achieved maximum accuracy of 66% using binary profile of nucleotide sequence taken from 5p arm of hairpin. (biomedcentral.com)
Earlier studies systematically analyzed sequence variations in human pri-miRNAs/pre-miRNA and experimentally discovered that single nucleotide polymorphisms (SNPs) in miR-125a obstruct the processing of pri-miRNA to pre-miRNA [ 16 ]. (biomedcentral.com)

CRISPR/Cas9-based genome-wide screening of the deubiquitinase subfamily identifies USP3 as a protein stabilizer of REST blocking neuronal differentiation and promotes neuroblastoma tumorigenesis. (nih.gov)
PASS2 version 6: a database of structure-based sequence alignments of protein domain superfamilies in accordance with SCOPe. (ncbs.res.in)
Based on the protein-protein interaction (PPI) network analysis, core targets and core subsets were screened. (hindawi.com)
COBALT computes a multiple protein sequence alignment using conserved domain and local sequence similarity information. (nih.gov)
COBALT is a multiple sequence alignment tool that finds a collection of pairwise constraints derived from conserved domain database, protein motif database, and sequence similarity, using RPS-BLAST, BLASTP, and PHI-BLAST. (nih.gov)
The first three proposals, on protein-based diagnosis, diagnostic materials and assays for less reactogenic vaccines, have been approved for one year. (who.int)
The tests used for protein C testing can include enzyme-linked immunosorbent assay (ELISA), chromogenic assays, and the activated partial thromboplastin time (aPTT)-based functional protein C assay. (medscape.com)
A thrombin-generation-based test has also been shown to detect protein C deficiency. (medscape.com)
The aPTT-based functional protein C assay may yield misleadingly low protein C levels in the presence of (1) a factor V Leiden mutation and some other causes of activated protein C resistance, (2) elevated plasma factor VIII levels, or (3) hyperlipidemia. (medscape.com)

Using terial infection was diagnosed in these patients because polymerase chain reaction (PCR) technology on fine nee- either acid-fast staining results were positive or the dle aspirates and biopsied specimens from 89 children with Mycobacterium genus-specific real-time polymerase chain cervicofacial lymphadenitis, we assessed the importance of M. haemophilum . (cdc.gov)
Biopsied specimens are subjected to acid-fast er (ITS) sequencing. (cdc.gov)
Additionally, a BLAST search in a reference database is often performed to identify sequences of type specimens to compare the samples within the phylogeny. (apsnet.org)

Some DNA- or RNA-binding enzymes can recognize specific base pairing patterns that identify particular regulatory regions of genes. (wikipedia.org)
These mechanical features are reflected by the use of sequences such as TATAA at the start of many genes to assist RNA polymerase in melting the DNA for transcription. (wikipedia.org)
Methods A next-generation sequencing (NGS) panel was created for the human TRPV1 gene and in addition, for the leukotriene receptors BLT1 and BLT2 recently described to modulate TRPV1 mediated sensitisation processes rendering the coding genes LTB4R and LTB4R2 important co-players in pharmacogenetic approaches involving TRPV1. (researchgate.net)
The NGS workflow was based on a custom AmpliSeq™ panel and designed for sequencing of human genes on an Ion PGM™ Sequencer. (researchgate.net)
This identified approximately 140 chromosome loci where nucleotides deviated from the reference sequence GRCh37 hg19 comprising the three genes TRPV1, LTB4R and LTB4R2. (researchgate.net)
It is suitable for large-scale sequencing of TRPV1 and functionally related genes. (researchgate.net)
2015. Genome sequencing of herb Tulsi (Ocimum tenuiflorum) unravels key genes behind its strong medicinal properties. . (ncbs.res.in)
Based on the attributes of the nodes in the network, the genes associated with the disease will be found. (hindawi.com)
This study is the first report on identifying bolting and flowering-related lncRNAs based on transcriptome sequencing in spinach, which provides a useful resource for future functional genomics studies, genes expression researches, evaluating genes regulatory networks and molecular breeding programs in the regulation of the genetic mechanisms related to bolting in spinach. (biomedcentral.com)
Results from RNA sequencing revealed that AEG‑1 modulated neuronal morphology and development by regulating the expression of numerous genes, such as ubiquitin C, C‑X‑C motif chemokine ligand 1, MMP9, Notch1, neuropilin 1 and ephrin type‑A receptor 4. (spandidos-publications.com)
We developed TS-REX, a database/software system that supports the analysis of tissue and cell type-specific transcription factor-gene networks based on expressed sequence tag abundance of transcription factor-encoding genes in UniGene EST libraries. (lu.se)

InsertionMapper: a pipeline tool for the identification of targeted sequences from multidimensional high throughput sequencing data. (genomeweb.com)
The completion of the first human genome drafts (Yamey 2000 ) was just a start of the modern DNA sequencing era which resulted in further invention, improved development toward new advanced strategies of high-throughput DNA sequencing, so called the " high-throughput next generation sequencing " (HT-NGS). (springer.com)
This preparation is optimized to work with PerkinElmer's automated magnetic bead-based DNA/RNA separation technology for low, medium and high-throughput solutions. (selectscience.net)
We recently developed a microfluidic system that enables the high-throughput sequencing of cytoplasmic and nuclear RNA with the physical fractionation of the RNA species via electrical lysis and isotachophoresis. (riken.jp)
The rapid development of gene chip high-throughput sequencing technology has accumulated much disease-related data since the past ten years. (hindawi.com)

Application of genotyping-by-sequencing on semiconductor sequencing platforms: a comparison of genetic and reference-based marker ordering in barley. (genomeweb.com)
Characterizing the genetic basis of transcriptome diversity through RNA-sequencing of 922 individuals. (genomeweb.com)
For the future direction, we need the in-depth genome sequence information and analysis for most of the mammals, including human to fully understand genome variation of economic traits, genetic susceptibility to diseases, and pharmacogenomics of drug response. (springer.com)
Xanthine is not part of the genetic code, but it is found in living organisms from the breakdown of guanine, which is a base in DNA and RNA. (creation.com)
Registration of 252 sequenced sorghum mutants as a community reverse genetic resource. (usda.gov)
Metagenomic sequencing engables a thorough mapping of the genetic material in the clinical sample, providing a broader understanding of the microbiological profile. (folkhalsomyndigheten.se)
By sequencing the entire genome, a comprehensive understanding of the genetic composition is obtained. (folkhalsomyndigheten.se)
In epidemiological contact tracing, this technology enables the mapping of infection spread and identification of sources of infection through the comparison of genetic sequences. (folkhalsomyndigheten.se)

The origin of biased sequence depth in sequence-independent nucleic acid amplification and optimization for efficient massive parallel sequencing. (genomeweb.com)
The Public Health Agency's Next Generation Sequencing (NGS) platform performs massive parallel sequencing of DNA and RNA. (folkhalsomyndigheten.se)

1988) Direct sequencing of polymerase chain reaction amplified DNA fragments through the incorporation of deoxynucleoside alpha-thiotriphosphates. (jenabioscience.com)

Cultures or species-specific genus-specific real-time PCR in combination with amplicon real-time PCR for M. avium and M. tuberculosis remained sequencing and a M. haemophilum -specific PCR resulted negative. (cdc.gov)
For example, AT-rich native-state DNA oligonucleotides often need to be over 30 bases long (sometimes over 40 bases) to satisfy amplicon design guidelines but may still perform poorly. (sigmaaldrich.com)

Novel CIC point mutations and an exon-spanning, homozygous deletion identified in oligodendroglial tumors by a comprehensive genomic approach including transcriptome sequencing. (genomeweb.com)

Sequences may or may not be amplified in vitro. (msdmanuals.com)

We describe isogenic colony sequencing (ICO-seq), a massively-parallel strategy to assess the gene expression profiles of large numbers of genetically distinct yeast colonies. (rsc.org)
The company undertakes the development, manufacture, and marketing of gene therapies and DNA vaccines for DNA amplification and sequencing. (globaldata.com)
Characterizing embryonic gene expression patterns in the mouse using nonredundant sequence-based selection. (nih.gov)
Often, sequence data of a single barcode gene is not sufficient to specifically determine fungal identity at the species level, whereas a combination of three or more barcodes can reliably determine which species the sample belongs to (see, e.g. (apsnet.org)
A novel species-specific 14-bp PNA probe was designed for M. immunogenum based on its 16S rRNA gene sequence and was validated for specificity, by testing against a panel of other phylogenetically closely related rapidly growing mycobacteria and representative species of gram-positive, gram-negative, and acid fast organisms. (cdc.gov)
The laboratory procedure is based on amplifying and sequencing four variable regions of the 16S rRNA gene with two amplicons. (folkhalsomyndigheten.se)

They provided a slight increase in sensitivity, which however does not warrant the additional computational cost of running the offline base caller. (biomedcentral.com)

The ability of oligonucleotides to discriminate between alleles via SNPs is greatly enhanced by the incorporation of Locked Nucleic Acid bases 6-8 ( Figure 2 ). (sigmaaldrich.com)
Moreover, the incorporation of quality scores is most appropriate for Illumina sequencing data. (biomedcentral.com)

One of the first discoveries was amino acids, the components of proteins. (creation.com)
The discipline of biophotonics deals with the interaction of light, or electromagnetic radiation, with living organisms and biologically active macromolecules: proteins (hemoglobin), nucleic acids (DNA and RNA), and metabolites (glucose and lactose). (nationalacademies.org)
RStrucFam: a web server to associate structure and cognate RNA for RNA-binding proteins from sequence information. (ncbs.res.in)

Nucleic Acids Res, 2022 Jun 10. (nih.gov)

The prediction of transcription factor binding sites in genomic sequences is in principle very useful to identify upstream regulatory factors. (lu.se)

Today, a number of tools exist for each of the steps for multigene phylogenetic analyses, including quality control and trimming, the generation of a multiple sequence alignment (MSA), extraction of informative sites, and the construction of the final phylogenetic tree. (apsnet.org)
Several different NGS-based analyses are offered within the Agency's specialised diagnostics. (folkhalsomyndigheten.se)

Subcellular RNA sequencing reveals broad presence of cytoplasmic intron-sequence retaining transcripts in mouse and rat neurons. (genomeweb.com)

Pink) mutant DNA analysis with Locked Nucleic Acid mutant probe (16mer with 3 Locked Nucleic Acid bases). (sigmaaldrich.com)
Purple) wild-type DNA with Locked Nucleic Acid mutant Probe (16mer with 3 Locked Nucleic Acid bases). (sigmaaldrich.com)

In a non-biological context, secondary structure is a vital consideration in the nucleic acid design of nucleic acid structures for DNA nanotechnology and DNA computing, since the pattern of basepairing ultimately determines the overall structure of the molecules. (wikipedia.org)
Strand separation by gentle heating, as used in PCR, is simple providing the molecules have fewer than about 10,000 base pairs (10 kilobase pairs, or 10 kbp). (wikipedia.org)
The double helix is an important tertiary structure in nucleic acid molecules which is intimately connected with the molecule's secondary structure. (wikipedia.org)
Nucleic acid cytometry using droplet microfluidics identifies and sorts single cells, virus, or free molecules based on specific "keyword" sequences. (rsc.org)
The capture oligomers contain sequences complementary to specific regions of the target molecules as well as a string of deoxyadenosine residues. (cdc.gov)

Deep-sequencing allows for an in-depth characterization of sequence variation in complex populations. (biomedcentral.com)

Nucleic acid mapping to Plasmodium was also used on the patient samples, confirming results obtained with an antigen-based rapid diagnostic test (RDT) conducted in the field laboratories. (asm.org)

13 The sequence reads were mapped onto the human reference genome, assembly GRCh37/hg19, with decoy sequences (hs37d5) and an average sequencing coverage of 32.4× for full-length autosomal chromosomes. (nature.com)

Locked Nucleic Acid in PCR primers, qPCR probes, and other types of oligonucleotides is soluble in water and standard buffers as well as follows Watson-Crick base-pairing rules. (sigmaaldrich.com)

Haplotype estimation using sequencing reads. (genomeweb.com)
The test data download unpacks into a directory named test_MDS_data containing fastq sequence reads and sam files from bismark mapping. (otago.ac.nz)

Nous avons réalisé une étude prospective en recourant à la surveillance de la grippe basée sur des sites sentinelles dans le but de dépister le VRS grâce à la PCR en temps réel chez des patients souffrant d'infections respiratoires aiguës recrutés au cours de deux saisons (2014-2015 et 2015-2016). (who.int)

Variant callers for next-generation sequencing data: a comparison study. (genomeweb.com)
HPC-based genome variant calling workflow (HPC-GVCW). (usda.gov)

To make a reference panel of genomic variation for the Japanese population, we sequenced whole genomes of 1,070 cohort participants, and detected genomic variants including SNVs, indels and structural variants. (nature.com)
Whole-exome sequencing reveals a rapid change in the frequency of rare functional variants in a founding population of humans. (genomeweb.com)
During this period, tremendous success has been achieved in the fields of decoding of human genome, technological advancement of new era of human genome applications, toward personalized genomes and discovery of rare variants, leveraging genome sequencing to impact on cancer researches and mammalian evolution and population structure. (springer.com)
Standard genotyping such as direct PCR sequencing methods, however, only provides information on the most abundant sequence variants. (biomedcentral.com)

Microfluidics entrepreneur Mark Gilligan provides a perspective on the development of single-cell sequencing technologies. (rsc.org)

The COVID-19 nucleic acid amplification tests pipeline product market report provides comprehensive information about the pipeline products with a comparative analysis of the products at various stages of development and information about the clinical trials which are in progress. (globaldata.com)
Diagnosis of rabies based on clinical symptoms alone is difficult and unreliable, except when there are specific clinical signs of hydrophobia or aerophobia [3]. (scielo.br)
All nucleic acid in the clinical sample is sequenced and the resulting data is analysed by experienced bioinformaticians. (folkhalsomyndigheten.se)
The result of of metagenomic sequencing is the outcome of a collaborative effort between bioinformaticians and clinical microbiologists, where the clinical interpretation confirms and complements the technical analysis. (folkhalsomyndigheten.se)
16S sequencing is performed to determine which bacterial species are present in a clinical sample or isolate. (folkhalsomyndigheten.se)
The basis of the GENSAM project is the joint national management of clinical microbial sequencing data. (folkhalsomyndigheten.se)

The data suggested that a high Plasmodium load, as determined by sequence read depth, was associated with mortality and influenced the host response, whereas a lower parasite load did not appear to affect outcome. (asm.org)
An evaluation of allele frequency estimation accuracy using pooled sequencing data. (genomeweb.com)
Calculation of Tajima's D and other neutrality test statistics from low depth next-generation sequencing data. (genomeweb.com)
These steps involve manual inspection of the sequence quality, followed by manual data trimming. (apsnet.org)
Although the use of second best base calls appeared very promising in our data exploration phase, their utility was limited. (biomedcentral.com)
DMAP components can filter and process aligned bisulphite sequenced data to generate comprehensive reference methylomes in different units for any genome. (otago.ac.nz)
1: Chatterjee A, Stockwell P A, Rodger E J, and Morison I M, Comparison of alignment software for genome-wide bisulphite sequence data , Nucleic Acids Research Volume 40, Issue 10Pp. (otago.ac.nz)
We note that the experimental phases are not without error, and it is hoped that in the future a model-based error model (or similar) could be used to improve both the amplitudes and the phases of the data collected. (iucr.org)
However, using appropriately modified scattering factors allows the use of the existing Fourier-based techniques that were developed for the analysis of X-ray data. (iucr.org)
Bioinformatic data processing pipelines in support of next-generation sequencing-based HIV drug resistance testing: the Winnipeg Consensus. (cdc.gov)
Notably, we now provide family alignments based on four different representative proteome sequence data sets and a new interactive DNA search interface. (bvsalud.org)

See Edgar RC, Nucleic Acids Res 16:380-5, 2004, PMID: 14729922 for k-mer counting-based sequence similarity. (nih.gov)
The distance between two sequences is computed as a fraction of words that appear in both sequences with respect to number of all words in the longer sequence (similarly as in Edgar RC, Nucleic Acids Res 16:380-5, 2004, PMID: 14729922 ). (nih.gov)

Papers with an asterisk(*) are based on research conducted outside of RIKEN. (riken.jp)

It can be represented as a list of bases which are paired in a nucleic acid molecule. (wikipedia.org)
Click molecule labels to explore molecular sequence information. (nih.gov)

In molecular biology, two nucleotides on opposite complementary DNA or RNA strands that are connected via hydrogen bonds are called a base pair (often abbreviated bp). (wikipedia.org)
Melting is the process by which the interactions between the strands of the double helix are broken, separating the two nucleic acid strands. (wikipedia.org)
Helicases unwind the strands to facilitate the advance of sequence-reading enzymes such as DNA polymerase. (wikipedia.org)
Target amplification assays are based on the ability of complementary oligonucleotide primers to specifically anneal and allow enzymatic amplification of the target nucleic acid strands. (cdc.gov)

It also made recommendations for the complete genome sequencing of all currently retained isolates in both repositories. (who.int)

Ultra-sensitive sequencing reveals an age-related increase in somatic mitochondrial mutations that are inconsistent with oxidative damage. (genomeweb.com)

Molecular research professionals can leverage these solutions to significantly increase the quality and yield of their nucleic acids from FFPE samples in less than four hours for up to 96 samples. (selectscience.net)
MERS-CoV) via next generation sequencing (NGS) from found in Wuhan, but it would appear samples prepared for cultured virus or directly from samples received from molecular testing could be handled as would samples of several pneumonia patients. (who.int)
Nucleic acid-based (molecular) identification. (msdmanuals.com)

The secondary structures of biological DNAs and RNAs tend to be different: biological DNA mostly exists as fully base paired double helices, while biological RNA is single stranded and often forms complex and intricate base-pairing interactions due to its increased ability to form hydrogen bonds stemming from the extra hydroxyl group in the ribose sugar. (wikipedia.org)

Metagenomic sequencing is offered in cases where infection is suspected but other methods have failed to identify a pathogen. (folkhalsomyndigheten.se)

Locked Nucleic Acid Dual-Labeled Probes discriminate better than DNA Dual-Labeled Probes in SNP genotyping analysis9. (sigmaaldrich.com)

The dynamics of genome replication using deep sequencing. (genomeweb.com)

Previously, an attempt to characterize these in the recognition of M. haemophilum as the causative mycobacteria by sequence analysis of the genus-specific agent in 16 (18%) children with cervicofacial lymphadenitis. (cdc.gov)
Single-cell RNA-sequencing (scRNA-seq) has revolutionized biomedical research by enabling the in-depth analysis of cell-to-cell heterogeneity of tissues with unprecedented resolution. (rsc.org)
A comparison of next-generation sequencing and clone-based sequencing in analysis of HBV RT quasispecies heterogeneity. (genomeweb.com)
Sequence variations between different bacteria provide a unique identity, and the analysis of these sequence enables taxonomic placement. (folkhalsomyndigheten.se)

Nucleic acid secondary structure is the basepairing interactions within a single nucleic acid polymer or between two polymers. (wikipedia.org)
Nucleic acid secondary structure is generally divided into helices (contiguous base pairs), and various kinds of loops (unpaired nucleotides surrounded by helices). (wikipedia.org)

The method uses an internal control sequence (spike) which is amplified and sequenced simultaneously with the sample to facilitate the assessment of the bacterial content and to detect amplification-inhibiting factors in the sample that may lead to a negative result. (folkhalsomyndigheten.se)

Table 1 Increasing the number of Locked Nucleic Acid bases in an oligonucleotide increases the T m . (sigmaaldrich.com)
T m of duplex between an oligonucleotide and its complementary sequence. (sigmaaldrich.com)
The presence of a single base mismatch has a greater destabilizing effect on the duplex formation between an Locked Nucleic Acid oligonucleotide and its target than with a native-state DNA oligonucleotide. (sigmaaldrich.com)

All sequencing libraries were constructed based on PCR-free methods. (nature.com)
Nucleic Acid-Based Identification Methods for Infectious Disease Nucleic acid-based methods detect organism-specific DNA or RNA sequences extracted from the microorganism. (msdmanuals.com)
However, this method is limited because it, unlike some nucleic acid-based methods, is not readily deployable in the field. (msdmanuals.com)

A double helix is formed by regions of many consecutive base pairs. (wikipedia.org)

While in China, especially the Qinghai-Tibet Plateau (QTP) and adjacent regions, considerable research achievements have also been attained on inferring the Quaternary phylogeographic histories of plant species based on the approach of population genetics (e.g. (frontiersin.org)
Nucleic Acids Research , 50, 962-974. (nih.gov)

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