• This kit contains Calcein-AM and Propidium Iodide (PI) solutions, which stain viable and dead cells, respectively (Fig. 1). (dojindo.com)
  • 2. Stain dead cells with 0.1-10 μM PI solution to find a PI concentration that stains the nucleus only, not the cytosol. (dojindo.com)
  • Figure 3: Two parameter isometric displays of green and red fluorescence from 5000 bull sperm stained with Rhodamine 123 and Propidium Iodide (RI23/Pl). Sperm samples from known fertile (A) and less fertile (B) donors are shown. (purdue.edu)
  • The use of a nucleic acid staining fluorescent dye may help improve nuclei counting accuracy as only nucleic acids will be stained and not debris. (10xgenomics.com)
  • Apoptosis was measured by Hoechst 33258/propidium iodide double staining of nuclear chromatin and the formation of gaps into the lymphendothelial barrier in a three-dimensional co-culture model consisting of MCF-7 tumour cell spheroids and human lymphendothelial monolayers. (nature.com)
  • To date, the most common method of capturing cells is for the software to identify nuclei stained with a fluorescent dye 11 - 13 . (ersjournals.com)
  • A critical role of autophagy was identified in rat nucleus pulposus (NP) cells: Inhibition of autophagy suppresses, while activation of autophagy enhances, the catabolic effect of cytokines. (spandidos-publications.com)
  • It is also used in combination with propidium iodide (PI) to determine viability in eukaryotic cells. (wikipedia.org)
  • while nucleus of membrane-compromised cells will fluoresce red, a sign of cell death. (wikipedia.org)
  • Figure 4: Two parameter green (log) versus red (linear) fluorescence isometric displays of 10,000 cells collected from fertile (A) and less fertile (B) bull sperm stained with SYBRI4 and counterstained with Propidium Iodide (PI). (purdue.edu)
  • It reaches the nucleus by passing through disordered areas of dead cell membrane and intercalates with the DNA double helix of the cell to emit red fluorescence (excitation: 535 nm, emmision: 617 nm). (dojindo.com)
  • In order to confirm the number of 3 chromosomes with 18S-25S rDNA loci also interphase nuclei and chromosomes in meiosis studied by FISH. (nenno.it)
  • As expected 6 FISH signals were observed on interphase nuclei and 3 signals on chromosomes in TelophaseII (haploid chromosome number). (nenno.it)
  • Interphase nucleus showing 6 FISH signals (yellow/green). (nenno.it)
  • Challenging samples that contain high amounts of debris, or are small in size like isolated nuclei, may benefit from an experienced user performing a visual quality control check. (10xgenomics.com)
  • On Countess 3 FL, enabling FL-based counting is required for nuclei and fixed samples. (10xgenomics.com)
  • Nuclei can be tricky to count due to their small size, which can be further exacerbated if a lot of cellular debris is present. (10xgenomics.com)
  • To identify the nucleus, micronucleus, and cytoplasm, 4,6-diamidino-2-phenyl-indole (DAPI) or propidium-iodide was used. (cdc.gov)
  • The cytoplasm appeared faint green in slides counter stained with propidium-iodide. (cdc.gov)
  • Nucleophosmin (NPM) is a ubiquitously expressed nucleolar phoshoprotein which shuttles continuously between the nucleus and cytoplasm. (haematologica.org)
  • Three lung cancer cell lines showing primarily nuclear localization of Kaiso were selected to examine whether roles of Kaiso in cytoplasm and in nucleus are identical. (biomedcentral.com)
  • Fluorescent dyes with aromatic amino or guanidine groups, such as propidium iodide (PI), diaminophenylindole (DAPI), acridine orange (AO), and Hoechst dyes, interact with nucleotides to emit fluorescence. (dojindo.com)
  • Apoptosis induction in the TOF treated cells was detected using cell membrane integration assay in adherent cells, DNA fragmentation assay, acridine orange/propidium iodide staining in adherent cells, and disruption of mitochondrial membrane potential. (aacrjournals.org)
  • Cells and nucleus morphological changes for apoptosis were clearly evident in both AMGM and AMN3 cancer cells when treated with IC50 for 24 hr as assessed using acridine orange and propidium iodide staining. (aacrjournals.org)
  • 11. Comparison of two flow cytometric assays for cellular RNA--acridine orange and propidium iodide. (nih.gov)
  • Cell nuclei in the spheroid are stained with the fluorescent dye propidium iodide (red). (integrativemedicinecouncil.org)
  • A novel method for the determination of micronuclei in human exfoliated cells using fluorescent propidium-iodide (25535164) staining with fluorescence in-situ hybridization (FISH) with a centromeric probe was described. (cdc.gov)
  • Triple staining (propidium iodide, TUNEL, and anti-α-sarcomeric actin antibody) was performed. (jci.org)
  • Staining for propidium iodide and anti-α-sarcomeric actin antibody is shown by red, and that for TUNEL by green. (jci.org)
  • Reduced DNA content, or hypoploidy, as indicated by propidium iodide (PI) staining, is another easy procedure for detecting apoptosis. (nih.gov)
  • After running samples with Propidium Iodide the cytometer needs thorough cleaning with 10% bleach and distilled water! (yale.edu)
  • For this goal, we made use of Jurkat cells overexpressing antiapoptotic Bcl-2 or the corresponding empty vector control and determined the volume of hypodiploid nuclei in Nicoletti assay immediately after 24 h (Figure 9A). (calcimimeticagent.com)
  • Reliable segmentation of cell nuclei from three dimensional (3D) microscopic images is an important task in many biological studies. (biomedcentral.com)
  • We present a novel, fully automated method for the segmentation of cell nuclei from 3D microscopic images. (biomedcentral.com)
  • In addition to general purpose identification and demarcation of the nucleus in live-cell imaging experiments, SV40 NLS-GFP is extensively used for analysis of nucleocytoplasmic transport and nuclear envelope integrity. (thermofisher.com)
  • In the current study, Wakayama's team generate healthy cloned mice offspring from freeze dried somatic cell nuclei through an adapted nuclear transfer procedure. (genengnews.com)
  • Our data reveal that although some DNA abnormalities are observed in the process, freeze dried somatic cell nuclei can be used to generate blastocysts by nuclear transfer, and embryonic stem cell lines derived from these blastocysts yield donor nuclei that are capable of producing healthy, fertile cloned mice," the authors noted. (genengnews.com)
  • 4. Flow cytometric analysis of G1- and G2/M-phase subpopulations in mammalian cell nuclei using side scatter and DNA content measurements. (nih.gov)
  • This gradient diffusion procedure results in a gradient flow field, in which the gradient vectors are smoothly flowing towards or outwards from the centers of the nuclei. (biomedcentral.com)
  • The genome sizes of the B- and Q-types of the whitefly Bemisia tabaci (Gennnadius) were estimated using flow cytometry ( Drosophila melanogaster as the DNA reference standard and propidium iodide (PI) as the fluorochrome) and k-mer analysis. (frontiersin.org)
  • The canonical model for SCN timekeeping pivots around transcriptional/translational feedback loops (TTFL) whereby PERIOD (PER) and CRYPTOCHROME (CRY) clock proteins associate and translocate to the nucleus to inhibit their own expression. (bvsalud.org)
  • In the overlay image, a nucleus stained by both TUNEL and propidium iodide is shown by yellow. (jci.org)
  • Scale bar, 100 c, Image analysis of the percentage of propidium iodide positive nuclei in an experiment resembling those in b. a, Protein lysates were prepared at designated times from Ripk1 MEFs treated with mTNF (10 ng ml and analysed by western blot for NF kinase (NIK), TRAF2, cIAP1 and cFLIP. (refszatmar.eu)
  • It was designed specifically to segment nuclei in images where the nuclei are closely juxtaposed or touching each other. (biomedcentral.com)
  • Given this characteristic of the thresholding-based methods, they often have difficulties in segmenting images with clustered or juxtaposed nuclei. (biomedcentral.com)
  • ASAR6 RNA is synthesized by RNA Polymerase II, is not polyadenlyated, is restricted to the nucleus, and is subject to random mono-allelic expression. (prolekarniky.cz)