Nuclear rnas show clear subcellular localization localization of specific rnas. Medical search. Frequent questions

In molecular biology, an intrinsically disordered protein (IDP) is a protein that lacks a fixed or ordered three-dimensional structure, typically in the absence of its macromolecular interaction partners, such as other proteins or RNA. (wikipedia.org)
Nuclear magnetic resonance spectroscopy of proteins also demonstrated the presence of large flexible linkers and termini in many solved structural ensembles. (wikipedia.org)
In the 2010s it became clear that IDPs are common among disease-related proteins, such as alpha-synuclein and tau. (wikipedia.org)
Nanos are RNA-binding proteins playing crucial roles in germ cell development and maintenance. (nature.com)
The Nanos proteins family regroups highly conserved RNA-binding proteins in higher eukaryotes implicated in germ cell development and maintenance. (nature.com)
It has been previously shown that rbf1 pro-apoptotic activity depends on its ability to decrease the level of anti-apoptotic proteins such as the Bcl-2 family protein Buffy. (sdbonline.org)
Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing. (nih.gov)
In the central dogma RNA acts as a simple intermediary between the DNA that carries the genetic information and the proteins that define the whole variety of biological processes in the cell. (biomedcentral.com)
Blocking the interaction between PIF7 and 14-3-3 proteins or reducing the expression of 14-3-3 proteins accelerates shade-induced nuclear localization and de-phosphorylation of PIF7, and enhances the shade phenotype. (elifesciences.org)
These studies have revealed that 14-3-3 proteins can interact with the phosphorylated forms of their client proteins in response to certain signals, and that this binding finalizes the signaling event by enabling a change in the subcellular localization, protein stability or intrinsic enzymatic activity of the client, or by promoting an association between the client and other proteins. (elifesciences.org)
Following the isolation of trypsinogen and chymotrypsinogen as specific proteins, DNase 1 was isolated in crystalline form from bovine pancreas and shown to be a heat sensitive protein that could cleave thymus DNA (16). (pancreapedia.org)
First, we co-expressed fluorescently tagged VAPYRIN with a range of subcellular marker proteins, and secondly, we employed biochemical tools to identify interacting partner proteins of VAPYRIN. (frontiersin.org)
As an important tool for the quantitative analysis of confocal microscopic data sets from co-expression of fluorescent proteins, we developed a semi-automated image analysis pipeline that allows for precise spatio-temporal quantification of protein co-localization and of the dynamics of organelle association from movies. (frontiersin.org)
We examined each of the individual SARS-CoV-2 proteins for their cytotoxicity in HEK 293 T cells and their subcellular localization in COS-7 cells. (biomedcentral.com)
We also used Mass-Spec Affinity purification to identify the host proteins interacting with SARS-CoV-2 Orf6 protein and tested a drug that could inhibit a specific Orf6 and host protein interaction. (biomedcentral.com)
All three proteins showed membrane localization in COS-7 cells. (biomedcentral.com)
In contrast, both proteins were jointly detectable in most CCA cell lines and showed nuclear/cytoplasmic shuttling in a cell density-dependent manner. (biomedcentral.com)
The nuclear genome encodes most of the chloroplast proteins, among which a large percentage of membrane proteins have yet to be functionally characterized. (biomedcentral.com)
We report here functional characterization of two nuclear-encoded chloroplast proteins, Chloroplast protein for Growth and Fertility (CGF1) and CGF2. (biomedcentral.com)
This study reports that two nuclear-encoded chloroplast proteins, Chloroplast protein for Growth and Fertility (CGF1) and CGF2, play important roles in vegetative growth, in female gametogenesis, and in embryogenesis likely by mediating chloroplast integrity and development. (biomedcentral.com)
Chloroplast genomes only encode about 80 to 100 proteins, while between 2500 and 3500 nuclear-encoded proteins are predicted to be targeted to chloroplasts [ 1 ]. (biomedcentral.com)
Chloroplast proteins encoded by the nuclear genome often play critical roles in maintaining chloroplast development and activity. (biomedcentral.com)
Transcriptional regulators or transcription factors (TFs) are proteins that bind to specific sequences of the DNA, i.e. promoters, and hereby facilitate or inhibit the binding of RNA polymerase (RNAP). (hormonessignaling.com)
compared to the other strains L. sakei MF1053 showed a higher expression of seven proteins related to stress whatever the carbon source used for growth (Figure 1c). (hormonessignaling.com)

This enzyme displays a DNA polymerase activity that can copy either DNA or RNA templates, and a ribonuclease H (RNase H) activity that cleaves the RNA strand of RNA-DNA heteroduplexes in a partially processive 3' to 5' endonucleasic mode. (proteopedia.org)
It is not clear if both polymerase and RNase H activities are simultaneous. (proteopedia.org)
RNase H probably can proceed both in a polymerase-dependent (RNA cut into small fragments by the same RT performing DNA synthesis) and a polymerase-independent mode (cleavage of remaining RNA fragments by free RTs). (proteopedia.org)
During productive infection, the 152-kb double-stranded HSV-1 genome is rapidly translocated to the nucleus where at least 80 viral genes are transcribed by the host cell RNA polymerase II (Pol II) [ 1 ]. (biomedcentral.com)

Recent studies showed the key role of lncRNAs in a variety of fundamental cellular processes. (biomedcentral.com)
The second group includes rRNAs and long non-coding RNAs (lncRNAs), which to date are very poorly functionally annotated. (biomedcentral.com)
In-depth analysis revealed that lncRNAs is an extensive and very heterogenic group from mRNA-like transcripts to circular RNAs. (biomedcentral.com)
According to the GENCODE project one third of all human lncRNAs genes are primate-specific [ 4 ]. (biomedcentral.com)
For example, XIST (X-inactive specific transcript), one of the first described lncRNAs has a low level of sequence conservation, but a highly conserved function across placental mammals - inactivation of the X chromosome [ 5 ]. (biomedcentral.com)
Some miRNAs are hosted in pri-miRNAs annotated as long non-coding RNAs (lncRNAs) and defined as MIRHGs (for miRNA Host Genes). (mdpi.com)
Recent studies have revealed the importance of long noncoding RNAs (lncRNAs) as tissue-specific regulators of gene expression. (nature.com)
We determine a comprehensive map of lineage-specific lncRNAs in human dermal lymphatic and blood vascular endothelial cells (LECs and BECs), combining RNA-Seq and CAGE-Seq. (nature.com)
Subsequent antisense oligonucleotide-knockdown transcriptomic profiling of two LEC- and two BEC-specific lncRNAs identifies LETR1 as a critical gatekeeper of the global LEC transcriptome. (nature.com)
In the latest genome annotation, lncRNAs, which are arbitrarily defined as noncoding RNAs longer than 200 nucleotides, constitute ~72% of the transcribed genome 13 , whereas mRNAs comprise only 19%, indicating the need for functional annotation of lncRNAs. (nature.com)
Long non-coding RNAs (lncRNAs) may act as potential markers for prognosis and progression of liver diseases and furthermore as direct targets for therapeutic purposes. (biomedcentral.com)

This class of noncoding RNAs is small, single stranded, and 19-25 nucleotide long that act as negative regulators involved in posttranscriptional silencing of the gene expression [ 5 ]. (hindawi.com)
They can bind to the 3′ untranslated region (3′UTR) of target messenger RNAs (mRNAs) in order to regulate gene expression post-transcriptionally with no sequence specificity but by potential electrostatic interactions with the phosphate backbone of RNA 2 , 3 . (nature.com)
Phosphorylation-resistant site mutations in PIF7 result in increased nuclear localization and shade-induced gene expression, and consequently augment hypocotyl elongation. (elifesciences.org)
Transcriptome comparisons of SOX9+ cells with GLT1+ cells showed that the two populations of cells exhibit largely overlapping gene expression. (jneurosci.org)
In particular, the FANTOM (Functional Annotation of the Mammalian Genome) consortium pioneered the discovery of the noncoding RNA world by providing, through Cap Analysis of Gene Expression (CAGE-Seq), the first evidence that large portions of our genome are transcribed, producing a multitude of sense and antisense transcripts 12 . (nature.com)
In contrast, the 'facultative' heterochromatin corresponds to the deposition of H3K27me3 on gene-rich regions, whose silencing is transient and dynamic across developmental processes, allowing cell type-specific differentiation and rapid adaptation of gene expression [ 5 ]. (biomedcentral.com)
Statistical analysis of gene expression data [10] showed that ArcA regulates the expression of a wide variety of genes learn more involved in the biosynthesis of small and macromolecules, transport, carbon and energy metabolism, cell structure, etc. (hormonessignaling.com)

Thousands of long non-coding RNA (lncRNA) genes are annotated in the human genome. (biomedcentral.com)
Generally, lncRNA has a lower expression level, their expression is more tissue-specific, and the majority of lncRNA has nuclear localization. (biomedcentral.com)
Deep RNA-DNA, RNA-protein interaction studies, and phenotype rescue analyses reveal that LETR1 is a nuclear trans-acting lncRNA modulating, via key epigenetic factors, the expression of essential target genes, including KLF4 and SEMA3C , governing the growth and migratory ability of LECs. (nature.com)
Together, our study provides several lines of evidence supporting the intriguing concept that every cell type expresses precise lncRNA signatures to control lineage-specific regulatory programs. (nature.com)
This study was intended to identify the function of long non-coding RNA (lncRNA) lncARSR in NAFLD and its role in human HCC cells (HepG2) proliferation and invasion. (biomedcentral.com)

In Vertebrates, Nanos paralogous genes were associated to partial redundancies and specific functional evolutions. (nature.com)
In teleostean fish, two nanos1 genes were generated, probably during the Teleost specific whole genome duplication (3R). (nature.com)
Catalytically active Dicer was purified from human nuclei and shown to promote processing of dsRNA hairpin structures [ 9 ] and stimulate initiation of RNAPII transcription at hormone-responsive genes [ 10 ]. (prolekarniky.cz)
In addition, nuclear Dicer fosters termination of RNAPII transcription [ 11 ] and alternative polyadenylation at a subset of protein-coding genes [ 12 ]. (prolekarniky.cz)
Colonization of the gut starts just after birth when pioneering species interact, through surface receptors, with gut cells to promote the expression of a specific set of host genes and favour the colonization of commensal microorganisms [6]. (deepdyve.com)
Tβ4-knockout B16F1 (Tβ4 KO) clones show significantly diminished expression level of tumor-associated genes, which is regulated by the TGFβ/MRTFs pathway. (aacrjournals.org)
Transcription analysis showed that the expression levels of the HpDGAT2A , HpDGAT2D , and HpDGAT2E genes markedly increased under high light and nitrogen deficient conditions with distinct patterns, which led to significant TAG and EAST accumulation. (researchsquare.com)
These results (1) update the gene models of HpDGAT2s , (2) prove the TAG biosynthesis capacity of HpDGAT2s, (3) show the strong preference for MUFAs and PUFAs, and (4) offer target genes to modulate TAG biosynthesis by using genetic engineering methods. (researchsquare.com)
Examination of the dJmj localization in various Polycomb group (PcG) mutant lines at the late growth stage allowed identification of some PcG genes, including Polycomb (Pc) , to be responsible for dJmj recruitment to nucleolus. (sdbonline.org)

To identify a nuclear marker pathognomonic of astrocytic phenotype, we assessed differential RNA expression by FACS-purified adult astrocytes and, on that basis, evaluated the expression of the transcription factor SOX9 in both mouse and human brain. (jneurosci.org)
A clear genotype/phenotype relationship has not been established, nor is it definitively known that alterations in transcription of Thap1 downstream targets are responsible for DYT6, as Thap1 may have other, yet to be identified functions. (biomedcentral.com)
ESE-1 initiates transformation of MCF-12A cells via a non-transcriptional, cytoplasmic process that is mediated by a unique 40-amino acid serine and aspartic acid rich (SAR) subdomain, whereas, ESE-1's nuclear transcriptional property is required to maintain the transformed phenotype of MCF7, ZR-75-1 and T47D breast cancer cells. (biomedcentral.com)

Functional complementation demonstrated that HpDGAT2A, HpDGAT2B, HpDGAT2D, and HpDGAT2E had the capacity to restore TAG synthesis in a TAG-deficient yeast strain (H1246) showing a large difference in enzymatic activity. (researchsquare.com)
In support of this notion, cells lacking a functional BRCA2 gene show hypersensitivity to DNA damaging agents such as mitomycin C (MMC) and sensitivity to chemicals such as methyl methane sulfonate [ 10 ]. (ijbs.com)
To map the minimal functional nuclear localization (NLS) and nuclear export (NES) signals, we fused in-frame putative NLS and NES motifs between GFP and the SAR domain. (biomedcentral.com)

POL_HV1B5 Gag-Pol polyprotein and Gag polyprotein may regulate their own translation, by the binding genomic RNA in the 5'-UTR. (proteopedia.org)
In addition to the catalytic domain, eukaryotic sirtuins contain variable amino- and carboxy-terminal extensions that regulate their subcellular localizations and catalytic activity. (biomedcentral.com)
The human ETS (E26 Transformation-Specific) protein family is a diverse group of 27 known transcription factors that regulate such varied cellular processes as differentiation and apoptosis, but also appear to induce oncogenesis when mutated or aberrantly expressed [ 1 - 4 ]. (biomedcentral.com)

Notably, Debcl modulates Drp1 mitochondrial localization during apoptosis. (sdbonline.org)
Taken together, these data show that the CDT of enterohepatic Helicobacters modulates the expression of the MAFB oncoprotein, which is translocated in the nucleus and is associated with the remodeling of the nuclei and actin cytoskeleton. (bvsalud.org)

Using peptide N-glucosidase F to remove the sugar chains, the enzymatic activity of human DNase 1 was shown to be decreased and the enzyme was more susceptible to heat (4). (pancreapedia.org)
Site directed mutagenesis has shown the importance of both glycosylation sites to maximum enzymatic activity, heat stability and resistance to trypsin digestion (5). (pancreapedia.org)

One of the most significant differences was the overexpression, in nonresponders, of CHEK1, a gene encoding Chk1, a protein kinase that can be blocked by specific inhibitors. (bvsalud.org)

MicroRNAs (miRNAs) are small regulatory non-coding RNAs, resulting from the cleavage of long primary transcripts (pri-miRNAs) in the nucleus by the Microprocessor complex generating precursors (pre-miRNAs) that are then exported to the cytoplasm and processed into mature miRNAs. (mdpi.com)
The cleavage is not sequence or base specific, but the rate of cutting is sequence specific (34). (pancreapedia.org)

Here, we show that shade light induces the de-phosphorylation and nuclear accumulation of PIF7. (elifesciences.org)
LncARSR was observed to bind to YAP1, which inhibited phosphorylation nuclear translocation. (biomedcentral.com)

From transcription to transport: emerging roles for nuclear myosin I. Hofmann WA, et al . (nih.gov)

Nanos family is characterized by two specific Cys-Cys-His-Cys zinc finger motifs at the carboxy-terminal region which present a regular spacing between the Cys and the His residues. (nature.com)
We observe that each gene and its products have a unique set of DNA, RNA or protein motifs that encode a regulatory program to define the logical circuitry that guides the life cycle of these biomolecules, from transcription to degradation. (biomedcentral.com)

Cytoplasmic Dicer is a key component of the canonical micro (mi)RNA biogenesis pathway. (prolekarniky.cz)
employed an endogenously HA-tagged Dicer knock-in mouse cell line to show that Dicer is exclusively cytoplasmic. (prolekarniky.cz)
This surprisingly wide range of YAP positivity in independent CCA cohorts might be due to different scoring algorithms (e.g., if only nuclear and/or cytoplasmic YAP positivity was considered) or variable cohort composition with regard to CCA subtypes. (biomedcentral.com)

These data highlight that ESE-1 contains NLS and NES signals that play a critical role in regulating its subcellular localization and function, and that an intact SAR domain mediates MEC transformation exclusively in the cytoplasm, via a novel nontranscriptional mechanism, whereby the SAR motif is accessible for ligand and/or protein interactions. (biomedcentral.com)

At low concentration, Gag-Pol and Gag would promote translation, whereas at high concentration, the polyproteins encapsidate genomic RNA and then shutt off translation (By similarity). (proteopedia.org)
Capsid protein p24 forms the conical core that encapsulates the genomic RNA-nucleocapsid complex in the virion. (proteopedia.org)
Nucleocapsid protein p7 encapsulates and protects viral dimeric unspliced (genomic) RNA. (proteopedia.org)
Facilitates rearangement of nucleic acid secondary structure during retrotranscription of genomic RNA. (proteopedia.org)
Conversion of viral genomic RNA into dsDNA requires many steps. (proteopedia.org)

The second function is to play a role in nuclear localization of the viral genome at the very start of cell infection. (proteopedia.org)
Reverse transcriptase/ribonuclease H (RT) is a multifunctional enzyme that converts the viral RNA genome into dsDNA in the cytoplasm, shortly after virus entry into the cell. (proteopedia.org)
A tRNA(3)-Lys binds to the primer-binding site (PBS) situated at the 5'-end of the viral RNA. (proteopedia.org)
The reading proceeds through the U5 region and ends after the repeated (R) region which is present at both ends of viral RNA. (proteopedia.org)
This ssDNA/tRNA hybridizes with the identical R region situated at the 3' end of viral RNA. (proteopedia.org)
As of November 2020, only 2 articles and 1 letter to the editor had been published documenting clear EM evidence of SARS-CoV-2 directly in tissue samples ( 30 - 32 ), and another 2 articles showed rare viral particles ( 33 , 34 ). (cdc.gov)
In addition, we provide a side-by-side comparison of the subcellular structures that have been most frequently misinterpreted as SARS-CoV-2 along with actual viral particles that have been identified in COVID-19 autopsy tissues. (cdc.gov)

Importantly, missense mutations have also been identified outside the DBD, and these mutations may alter Thap1 conformation and/or localization in such a way as to indirectly affect the structure and/or function of the DBD. (biomedcentral.com)
It really is plausible which the subcellular localization of CAPN3 determines its particular mobile function, which mutations make a difference some of those features straight or indirectly (1). (exposed-skin-care.net)

ROC analysis showed high diagnostic performance of miR-34a (AUC = 0.854), MET (AUC = 0.765), and E2F3 (AUC = 0.761). (hindawi.com)
Cell cycle analysis showed an accumulation of tumor cells predominantly in 511296-88-1 manufacture G0/G1 phase with an increase in concentration of TD-MSCs, which was confirmed by increased mRNA expression of cell cycle negative regulator p21. (immune-source.com)
The analysis showed that SOX9+ astrocytes constitute ∼10-20% of the total cell number in most CNS regions, a smaller fraction of total cell number than previously estimated in the normal adult brain. (jneurosci.org)

Analysis revealed that Buffy acted to rescue the associated loss of locomotor ability observed in the alpha-synuclein-induced model of PD, while Buffy RNA interference resulted in an enhanced alpha-synuclein-induced loss of climbing ability. (sdbonline.org)
Here, BRCA2 heterozygosity was mimicked in HT-29 colon cells by reducing levels of BRCA2 through stable RNA interference. (ijbs.com)
In this report, we have utilized HT-29 colon cancer cells and have mimicked the heterozygous state of BRCA2 in these cells through RNA interference. (ijbs.com)

The latter two studies conclude that Dicer association with chromatin may be mediated by the localised production of dsRNA, which is processed into endogenous small interfering (endo-si)RNA to mediate heterochromatin formation by recruitment of G9a methyltransferase in a Dicer-dependent manner. (prolekarniky.cz)

Murine skin melanoma cells (B16F0 and B16F1) also show the expression regulation of Tβ4 by Smad and TCF/LEF. (aacrjournals.org)

On the basis of cell line experiments, abiraterone acetate and Chk1 inhibitor combination showed at least additive effect on cell viability, cell cycle, apoptosis, and accumulation of DNA damages. (bvsalud.org)

First identified in Drosophila, the conserved Polycomb group (Pc-G) protein complexes were shown to be both writers (Polycomb Repressive Complex 2, PRC2) and readers (Polycomb Repressive Complex 1, PRC1) of H3K27me3 [ 19 , 20 , 21 ]. (biomedcentral.com)

RNase H digests the RNA template except for two polypurine tracts (PPTs) situated at the 5'-end and near the center of the genome. (proteopedia.org)
Large international consortiums such as ENCODE (The Encyclopedia of DNA Elements) has shown that up to 80% of the genome is transcribed while only 1,5% of it is protein -coding sequences [ 1 ]. (biomedcentral.com)

The goal of this study was to examine the nature and expression pattern of the protein product(s) of the Thap1 transcription factor ( DYT6 gene) in mouse neurons, and to study the regional and developmental distribution, and subcellular localization of Thap1 protein. (biomedcentral.com)

Although a direct link between Snail expression and tumor metastasis has not yet been reported, a number of studies have shown that the overexpression of Snail is correlated with tumor invasion ( 7 ). (spandidos-publications.com)

In summary, although garcinol and curcumin can both inhibit histone acetyltransferase activities, our results show that these compounds have differential effects on cancer cells in culture. (biomedcentral.com)

To assess the role of NES2 in regulating ESE-1 subcellular localization and subsequent transformation potency, we site-specifically mutagenized NES2, within full-length GFP-ESE-1 and GFP-NES2-SAR reporter constructs. (biomedcentral.com)

while Ms Moushami Mallik, BHU, threw light on various pathways through which a non-coding RNA could possibly ameliorate polyQ-induced neurodegeneration in Drosophila. (ifcbiol.com)

The endoribonuclease Dicer recognises and processes double-stranded (ds)RNA substrates of various origins into small non-coding (nc)RNA [ 1 ]. (prolekarniky.cz)

A series of studies imply nuclear localisation of mammalian Dicer and association with chromatin. (prolekarniky.cz)
More recently, two studies point toward Dicer-dependent nuclear RNAi in mammals by demonstrating that nuclear, chromatin-associated Dicer impairs expression of the microtubule-binding protein Doublecortin in mouse adult neural stem cells [ 14 ] and transactivation of the human secreted frizzled-related protein 1 promoter in cholangiocarcinoma cells [ 15 ]. (prolekarniky.cz)
In a mutant of testis-specific TAF homolog (tTAF) that is responsible for nucleolus localization of Pc, dJmj signals were detected not only at nucleolus but also on the condensed chromatin in the late growth stage. (sdbonline.org)
Mr S M Pandey, DU, demonstrated nucleosome remodeling by the SW1/SNF chromatin remodeling complex obtained from chicken liver nuclear extract. (ifcbiol.com)

Studies have shown a correlation between Snail expression and the degree of infiltration in breast carcinomas ( 8 ). (spandidos-publications.com)
A number of reports have shown that RhoA expression is upregulated in a group of malignancies ( 13 ) and that the activity of RhoA is correlated with lymph node metastasis in colorectal cancer ( 14 ). (spandidos-publications.com)
Finally, we show that the elevation of H4K20Me3 levels by garcinol correlated with increased expression of SUV420H2, and was prevented by siRNA targeting of SUV420H2. (biomedcentral.com)

Our data indicate evolutionary conservation of nuclear Dicer localisation and function in mammals in response to DNA damage. (prolekarniky.cz)

This paper challenges several studies reporting various RNA metabolic functions of Dicer in human nuclei. (prolekarniky.cz)
An analysis of MAFB protein subcellular localization revealed a strong nuclear and perinuclear localization in the CdtB-distended nuclei in intestinal and hepatic cells. (bvsalud.org)

The transcription factor Nuclear Factor-kappa B (NF-κB) is pivotal in mediating inflammatory processes in general and is considered as the central regulator activating cells in response to silica [ 6 ]. (biomedcentral.com)
The neural localization of the 50 kDa species and its nuclear compartmentalization suggests that these may be key Thap1 species controlling neuronal gene transcription. (biomedcentral.com)
Studies have shown that several transcription factors, including the Snail/Slug family ( 3 ), δEF1/ZEB1 ( 4 ) and SIP1 ( 5 ) have been implicated in E-cadherin repression. (spandidos-publications.com)
While previous publications have established ESE-1's transcription factor function, we have reported that ESE-1 initiates transformation of MECs via a novel non-nuclear, non-transcriptional mechanism [ 13 ]. (biomedcentral.com)

Binds these RNAs through its zinc fingers. (proteopedia.org)

We describe morphologic features of coronavirus that distinguish it from subcellular structures, including particle size range (60-140 nm), intracellular particle location within membrane-bound vacuoles, and a nucleocapsid appearing in cross section as dense dots (6-12 nm) within the particles. (cdc.gov)

Activation of the classical NF-κB pathway in macrophages and type II pneumocytes was indicated by enhanced immunostaining of phospho-IκBα in these specific lung cell types. (biomedcentral.com)

The sequencing result also showed that there was no mutation of amino acid sequences (data not shown). (biomedcentral.com)

Dr Somdatta Sinha, CCMB, gave a clear account highlighting protein modeling as a means to understanding structure and function. (ifcbiol.com)
Loss of heterozygosity at the 8-oxoG-DNA glycosylase (OGG1) allele is found in human kidney clear cell carcinoma identifying loss of OGG1 function as a possible contributor to tumorigenesis in the kidney. (biomedcentral.com)

However, a growing body of evidence points toward localisation and activity of mammalian Dicer in the nucleus. (prolekarniky.cz)
The Filipowicz lab reported enrichment of mammalian Dicer at ribosomal RNA loci, suggesting a possible role for Dicer in maintaining integrity of ribosomal DNA arrays. (prolekarniky.cz)
The BRCA2 protein has been shown to bind to RAD51, the mammalian homolog of the RecA recombinase [ 6 - 9 ], and thus is believed to be involved in the repair of DNA double-strand breaks [ 6 ]. (ijbs.com)

Overexpression of Snail has been associated with breast cancer metastasis, although the specific role of Snail in the process remains unclear. (spandidos-publications.com)
Overexpression of one ETS protein in particular, the epithelium-specific ETS factor ESE-1, is implicated in human mammary transformation. (biomedcentral.com)

Our goal is to highlight the importance of coronavirus morphology and cellular localization in diagnosis and detection. (cdc.gov)
Finally, US11 has been recently shown to also counteract the activity of the 2'-5' oligoadenylate synthetase (OAS), a cellular protein critical for host cell defense [ 15 ]. (biomedcentral.com)

We show here that SOX9 is an astrocyte-specific nuclear marker in all major areas of the CNS outside of the neurogenic regions. (jneurosci.org)

Knockdown experiments on human and mice lung cancer cell lines showed a decrease migration and metastatic rate in human. (biomedcentral.com)
SIGNIFICANCE STATEMENT Astrocytes are traditionally identified immunohistochemically by antibodies that target cell-specific antigens in the cytosol or plasma membrane. (jneurosci.org)
No difference in RAD51 subcellular localization and focus formation was observed between control and mimicked heterozygous cell lines. (ijbs.com)
In support of this hypothesis, heterozygosity for a BRCA2 mutation has been shown to cause sensitivity to DNA damage agents and reduced RAD51 focus formation after irradiation in the chicken B cell line DT40 [ 16 ]. (ijbs.com)
Jarid2 methylation is shown to be important to promote PRC2 activity at a locus devoid of H3K27me3 and for the correct deposition of this mark during cell differentiation. (sdbonline.org)
Cell viability/proliferation assays, cell cycle analysis by flow cytometry, immunodetection of specific histone and p53 acetylation marks, western blotting, siRNA and RT-qPCR. (biomedcentral.com)

The human BRCA2 gene encodes a nuclear protein of 3,418 amino acids [ 5 ], and is believed to play a pivotal role in DNA damage repair [ 6 ]. (ijbs.com)

Immunocytochemical analyses was performed with specific antibodies to dJmj and tri-methylation at lysine 27 on histone H3 (H3K27me3). (sdbonline.org)

This review is focused on hereditary diseases in the pathogenesis of which long non-coding RNAs play an important role. (biomedcentral.com)
Based on the length of the transcript non-coding RNAs are divided into two groups: short and long non-coding RNAs. (biomedcentral.com)

The authors showed that transfection of exogenous small interfering (exo-si)RNA triggers silencing of a subset of protein-coding gene promoters. (prolekarniky.cz)

In attempts to attribute pathology of COVID-19 patients directly to tissue damage caused by SARS-CoV-2, investigators have inaccurately reported subcellular structures, including coated vesicles, multivesicular bodies, and vesiculating rough endoplasmic reticulum, as coronavirus particles. (cdc.gov)

Human Dicer may also interact with the nuclear pore complex component NUP153 [ 7 ]. (prolekarniky.cz)

The Thap1 species at the 50 kDa size range was exclusively detected in murine brain and testes and were located in the nuclear compartment. (biomedcentral.com)

Moreover, we are yet unable to predict response to a specific therapy in an individual patient and the optimal sequence of these therapies needs to be clarified. (oaepublish.com)

Employing the same cells as used by Much and colleagues, we combined super-resolution microscopy followed by 3D reconstitution and biochemical assays to show that endogenously tagged HA-Dicer prominently localises in the nucleus under physiological conditions. (prolekarniky.cz)

Therefore, it is clear that US11 is a multifunctional protein involved in HSV-1 infection. (biomedcentral.com)

However, the role of lncARSR in NAFLD is less clear. (biomedcentral.com)

We generated the corresponding histone methyltransferase null mutants and showed an interdependence of H3K9me3 and H3K27me3 marks. (biomedcentral.com)

Although a report has shown that RhoB, a small GTPase involved in cytoskeletal actin rearrangement, lies downstream of Slug ( 11 ), another member of the Snail super-family, there is no evidence to support a link between Snail and Rho GTPases. (spandidos-publications.com)

Interestingly, Dicer depletion in human cells caused defects in precursor messenger (pre-m)RNA processing [ 8 ]. (prolekarniky.cz)
These findings are in line with previous studies, reporting the existence of nuclear RNAi in human cells [ 13 ]. (prolekarniky.cz)
Fatty acid (FA) profile assays revealed that HpDGAT2A, HpDGAT2D, and HpDGAT2E, but not HpDGAT2B, preferred monounsaturated fatty acyl-CoAs (MUFAs) for TAG synthesis in yeast cells, and showed a preference for polyunsaturated fatty acyl-CoAs (PUFAs) based on their feeding strategy. (researchsquare.com)
As shown for HCC, inactivation of the Hippo kinase cassette or activation of YAP is leading to liver overgrowth via expansion of liver progenitor cells and eventually cancer development with combined hepatocellular/cholangiocellular differentiation [ 5 ]. (biomedcentral.com)
Dr A Rai, BHU, showed how limbal stem cells can be transplanted to cure chemical injuries of the cornea. (ifcbiol.com)
However, most of the presumed virus or virus-like particles shown in all of these reports either represent normal subcellular organelles previously demonstrated in cells ( 23 ) or, otherwise, lack sufficient ultrastructure and morphologic features to be conclusively identified as coronavirus. (cdc.gov)
Western blot analysis demonstrated that the US11 protein was specifically recognized by the polyclonal antibody, and immunofluorescent assay also showed that the antibody was able to probe the US11 protein in the cells infected with HSV-1. (biomedcentral.com)

Anatomy45

Organisms19

Chemicals and Drugs84

Analytical, Diagnostic and Therapeutic Techniques and Equipment34

Psychiatry and Psychology1

Phenomena and Processes71

Disciplines and Occupations3

Technology, Industry, Agriculture1

Information Science7

Proteins24

Polymerase4

LncRNAs11

Gene expression7

LncRNA5

Genes9

Phenotype3

Functional3

Regulate3

Modulates2

Enzymatic activity2

Protein kinase1

Cleavage2

Phosphorylation2

Roles1

Motifs2

Cytoplasmic3

Interactions1

Genomic5

Viral7

Mutations2

Analysis showed3

Interference3

Heterochromatin1

Regulation1

Apoptosis1

Polycomb1

Genome2

Developmental1

Tumor1

Inhibit1

Subsequent1

Drosophila1

Endoribonuclease1

Chromatin4

Expression3

Evolutionary1

Nuclei2

Transcription4

Zinc1

Particle1

Pathway1

Sequences1

Function2

Mammalian3

Overexpression2

Cellular2

Marker1

Cell6

Encodes1

Analyses1

Long2

Authors1

Structures1

Complex1

Murine1

Sequence1

Biochemical1

Multifunctional1

Role1

Histone1

Small1

Cells7