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  • kinase
  • Binding of these factors is similar in nuclear extracts from liver which does or does not express the FAS gene as observed for glucose-responsive elements in the L-pyruvate kinase and S14 genes. (biochemj.org)
  • amino
  • We deduced the amino acid sequence of the chicken lamin B receptor from overlapping clones obtained by screening cDNA libraries with a probe generated by the polymerase chain reaction with primers based on the partial protein sequence of the isolated protein. (rupress.org)
  • mitotic
  • The RanGTPase is essential for three aspects of nuclear structure and function: nucleocytoplasmic transport, mitotic spindle formation, and the structure and post-mitotic re-assembly of the NE ( Sazer and Dasso, 2000 ). (biologists.org)
  • novel
  • Finally, we uncover a novel role of LEM-2 in nuclear separation and anchoring of microtubule organizing centers. (biologists.org)
  • We have developed a novel method, MCLIP ( Membrane protein Cross-Link ImmunoPrecipitation ), which takes advantage of a cell permeable crosslinker to enable effective detection and analysis of specific interactions of NE proteins in live cells using Western blot. (diva-portal.org)
  • interactions
  • In support of this hypothesis, we found that treatment of cells with leptomycin B, which disrupted Cyclin B1-CRM1 interactions, led to a marked nuclear accumulation of Cyclin B1. (duke.edu)
  • We also, show that MCLIP is suitable to coprecipitate protein interactions in different stages of the cell cycle. (diva-portal.org)
  • Protein-protein interactions in the NE have been difficult to study due to the resistance of NE proteins to extraction. (diva-portal.org)
  • steps
  • sns-A10, sns-B2 and sns-B9 have mutations in the fission yeast homologues of S. cerevisiae Sar1p, Sec31p and Sec53p, respectively, which are required for the early steps of the protein secretory pathway. (biologists.org)
  • The endoplasmic reticulum (ER) is the cytoplasmic organelle where the early steps of the protein secretory pathway are localized. (biologists.org)
  • region
  • DNase I footprinting experiments using a + 161 nt to + 405 nt fragment of the FAS gene demonstrate that a region from + 290 nt to + 316 nt is protected by nuclear extracts from liver and spleen. (biochemj.org)
  • bind
  • Nuclear import receptors such as importin beta bind their substrates only in the absence of GTP-bound RAN and release them upon direct interaction with GTP-bound RAN , while export receptors behave in the opposite way. (rcsb.org)
  • RNA-binding proteins (often abbreviated as RBPs) are proteins that bind to the double or single stranded RNA in cells and participate in forming ribonucleoprotein complexes. (wikipedia.org)
  • Organism
  • View all proteins of this organism that are known to be involved in the pathway proteasomal ubiquitin-dependent pathway and in Protein degradation . (uniprot.org)
  • p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence. (uniprot.org)
  • section shows the unique identifier assigned by the NCBI to the source organism of the protein. (uniprot.org)
  • Frequently, the interacting pair of proteins may have been identified by studies of human genetics, indicating the interaction can be disrupted by unfavorable mutations, or they may play a key role in the normal biology of a "model" organism like the fruit fly, yeast, the worm C. elegans, or mice. (wikipedia.org)
  • NPLOC4
  • The NPLOC4 - UFD1 - VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope (PubMed:10811609, PubMed:11740563, PubMed:11781570, PubMed:12411482, PubMed:12644454, PubMed:14636562). (rcsb.org)
  • eukaryotes
  • The nuclear cap-binding complex is a functional heterodimer and composed of Cbc1/Cbc2 in yeast and CBC20/CBC80 in multicellular eukaryotes. (wikipedia.org)
  • pathway
  • section describes the metabolic pathway(s) associated with a protein. (uniprot.org)
  • This protein is involved in the pathway proteasomal ubiquitin-dependent pathway, which is part of Protein degradation. (uniprot.org)
  • The nuclear scaffold is predicted to be relatively permanent for a given cell type, but induction of a signaling pathway-by ligand binding, cell:cell contact, or some other mechanism-can temporarily shift the expression profile. (wikipedia.org)
  • regulate
  • As nuclear RNA emerges from RNA polymerase, RNA transcripts are immediately covered with RNA-binding proteins that regulate every aspect of RNA metabolism and function including RNA biogenesis, maturation, transport, cellular localization and stability. (wikipedia.org)
  • Expression
  • This protein acts as a cyclin E-CDK2 phosphorylation substrate and its effects on cell-cycle progression are enhanced by the co-expression of cyclin E and CDK2. (cags.org.ae)
  • Inhibition by Impβ results from depletion nuclear RanGTP, and, in support of this mechanism, expression of mRFP-Ran reversed the inhibition. (rupress.org)
  • Once all regulatory proteins, etc. have been synthesized and the scaffold has been established, the cell has attained its own specific expression profile. (wikipedia.org)
  • This protein is thought to be critical to development of the dopamine phenotype in the midbrain, as mice without NURR1 are lacking expression of this phenotype. (wikipedia.org)
  • While NURR1 is a key protein, there are other factors required as research shows that solely expressing NURR1 fails to stimulate this phenotypic gene expression. (wikipedia.org)
  • In the rat, although the gene for PRM2 is present, expression of this protein is extremely small because of limited transcription due to an inefficient promoter in addition to altered processing of the mRNA transcript. (wikipedia.org)
  • scaffold
  • It demonstrates that the scaffold protein TAB1 can induce p38α autophosphorylation and leads to its activation. (biologists.org)
  • Thus, the nuclear scaffold places limits on what genes can and can not be expressed within a given cell and, hence, may serve a basis for cell identity. (wikipedia.org)
  • molecular
  • Immunoblotting of two-dimensional gel electrophoregrams of oocyte nuclear proteins showed that these antibodies recognized proteins whose relative molecular masses and isoelectric points were 80×103 and 6á4, 175×103 and 5 and 270×103 and 7, respectively. (biologists.org)
  • transcripts
  • The cap and nuclear cap-binding protein have many function in mRNA biogenesis including splicing, 3'-end formation by stabilizing the interaction of the 3'-end processing machinery, nuclear export and protection of the transcripts from nuclease degradation. (wikipedia.org)
  • however, some protein-encoding RNA transcripts have been shown to be subject to editing resulting in a difference in their protein's amino acid sequence. (wikipedia.org)
  • Therefore
  • Therefore, a procedure that does not include the use of detergents should be used for the preparation of nuclear proteins. (sigmaaldrich.com)
  • Unlike proteins and RNA, DNA usually lacks tertiary structure and therefore damage or disturbance does not occur at that level. (wikipedia.org)
  • Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions. (wikipedia.org)
  • HUMAN
  • Human FAM76B (hFAM76B) is a 39 kDa protein that contains homopolymeric histidine tracts, a targeting signal for nuclear speckles. (sigmaaldrich.com)
  • Due to the high conservation of protein sequences between mouse and human FAM76B, MAbs against hFAM76B were shown to react with mouse FAM76B (mFAM76B) specifically. (sigmaaldrich.com)
  • Human nuclear cap-binding protein complex shows the large subunit, CBC80 consists of 757 amino acid residues. (wikipedia.org)
  • Human nuclear cap-binding protein complex plays important role in the maturation of pre-mRNA and uracil-rich small nuclear RNA. (wikipedia.org)
  • The predicted secondary structures of the human protein were assessed by multiple bioinformatic tools. (wikipedia.org)
  • enzyme
  • The presence of this enzyme also results in the increased hardiness of a second protein, N-MYC, which has long been known to increase the aggressiveness of neuroblastoma when it is present in higher-than-normal amounts. (brightsurf.com)
  • So by limiting the effectiveness of the PRMT1 enzyme, researchers believe they can decrease the damage done by both proteins at once. (brightsurf.com)
  • Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. (wikipedia.org)
  • genomic
  • It is a regulatory mechanism by which variations in the incorporation of the exons into mRNA leads to the production of more than one related protein, thus expanding possible genomic outputs. (wikipedia.org)
  • receptor
  • Importin-β (Impβ) is a major transport receptor for Ran-dependent import of nuclear cargo. (rupress.org)
  • Among the few well-characterized INM proteins are lamin B receptor (LBR), the lamina-associated polypeptide 1 (LAP1), the lamina-associated polypeptide-2 (LAP2), emerin and MAN1. (wikipedia.org)
  • An example of this is the glutamate receptor mRNA where glutamine is converted to arginine leading to a change in the functionality of the protein. (wikipedia.org)
  • regulatory
  • Factors involved in nuclear transport have been well studied, but systems analysis can offer further insight into regulatory mechanisms. (rupress.org)
  • nucleotide
  • The protein encoded by this gene removes 5' overhanging "flaps" (or short sections of single stranded DNA that "hang off" because their nucleotide bases are prevented from binding to their complementary base pair-despite any base pairing downstream) in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. (wikipedia.org)
  • expression
  • Once all regulatory proteins, etc. have been synthesized and the scaffold has been established, the cell has attained its own specific expression profile. (wikipedia.org)
  • The nuclear scaffold is predicted to be relatively permanent for a given cell type, but induction of a signaling pathway-by ligand binding, cell:cell contact, or some other mechanism-can temporarily shift the expression profile. (wikipedia.org)
  • This protein is thought to be critical to development of the dopamine phenotype in the midbrain, as mice without NURR1 are lacking expression of this phenotype. (wikipedia.org)
  • genes
  • Thus, the nuclear scaffold places limits on what genes can and can not be expressed within a given cell and, hence, may serve a basis for cell identity. (wikipedia.org)
  • Eukaryotic cells encode diverse RBPs, approximately 500 genes, with unique RNA-binding activity and protein-protein interaction. (wikipedia.org)
  • domain
  • Proteins containing the LEM domain, such as emerin, LAP2β and MAN1, seem to have a number of roles. (wikipedia.org)
  • A nuclear localisation signal (NLS) is not sufficient to target a protein to the INM, and the N-terminal domain of LBR cannot translocate into the nuclear lumen if its size is increased from 22 to approximately 70 kDa, supporting this view. (wikipedia.org)
  • Each basic domain recognizes RNA, but many of these proteins require multiple copies of one of the many common domains to function. (wikipedia.org)
  • human
  • Human nuclear cap-binding protein complex shows the large subunit, CBC80 consists of 757 amino acid residues. (wikipedia.org)
  • Frequently, the interacting pair of proteins may have been identified by studies of human genetics, indicating the interaction can be disrupted by unfavorable mutations, or they may play a key role in the normal biology of a "model" organism like the fruit fly, yeast, the worm C. elegans, or mice. (wikipedia.org)
  • genetic
  • The source of the protein can be either natural or produced in a production system using recombinant DNA techniques through genetic engineering. (wikipedia.org)
  • indicates
  • Some binding proteins such as neuronal specific RNA-binding proteins, namely NOVA1, control the alternative splicing of a subset of hnRNA by recognizing and binding to a specific sequence in the RNA (YCAY where Y indicates pyrimidine, U or C). These proteins then recruit splicesomal proteins to this target site. (wikipedia.org)
  • sufficient
  • Recombinantly expressed proteins are usually easier to produce in sufficient quantity, and this method makes isotopic labeling possible. (wikipedia.org)
  • secondary
  • Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions. (wikipedia.org)
  • structure
  • There are about 60 INM proteins, most of which are poorly characterized with respect to structure and function. (wikipedia.org)
  • These distances in turn can be used to determine the overall structure of the protein. (wikipedia.org)
  • Unlike proteins and RNA, DNA usually lacks tertiary structure and therefore damage or disturbance does not occur at that level. (wikipedia.org)
  • complex
  • The splicesome is a complex of snRNA and protein subunits and acts as the mechanical agent that removes introns and ligates the flanking exons. (wikipedia.org)
  • consists
  • Usually, the sample consists of between 300 and 600 microlitres with a protein concentration in the range 0.1 - 3 millimolar. (wikipedia.org)
  • known
  • SR proteins are also well known for their role in alternative splicing through the recruitment of snRNPs that form the splicesome, namely U1 snRNP and U2AF snRNP. (wikipedia.org)
  • small
  • These non-coding RNAs include microRNAs, small interfering RNAs (siRNA), as well as splicesomal small nuclear RNAs (snRNA). (wikipedia.org)