• We determined that NUP88 and the nuclear transport factors NUP98 and RAE1 comprise a regulatory network that inhibits premitotic activity of the anaphase-promoting complex/cyclosome (APC/C). When overexpressed, NUP88 sequesters NUP98-RAE1 away from APC/CCDH1, triggering proteolysis of polo-like kinase 1 (PLK1), a tumor suppressor and multitasking mitotic kinase. (elsevier.com)
  • Surprisingly, NUP88 overexpression did not alter global nuclear transport, but was a potent inducer of aneuploidy and chromosomal instability. (elsevier.com)
  • p>When browsing through different UniProt proteins, you can use the 'basket' to save them, so that you can back to find or analyse them later. (uniprot.org)
  • GTPase involved in nucleocytoplasmic transport, participating both to the import and the export from the nucleus of proteins and RNAs (PubMed:10400640, PubMed:8276887, PubMed:8896452, PubMed:8636225, PubMed:8692944, PubMed:9351834, PubMed:9428644, PubMed:9822603, PubMed:26272610). (rcsb.org)
  • The distribution of this protein in the oocyte and its behaviour during development suggest that it might be involved in the packaging of RNAs during transcription. (biologists.org)
  • Many proteins and RNAs are translocated through the NPC by shuttling transport receptors of the karyopherin family (importins and exportins), which recognize NLSs or nuclear export signals on the cargo. (rupress.org)
  • B-WICH also contains RNAs, 45 S rRNA, 5 S rRNA, 7SL RNA, and traces of the U2 small nuclear RNA. (eurekamag.com)
  • Results showed that TP53INP1 and E-cadherin (zeige CDH1 ELISA Kits ) mRNA and protein were significantly down-regulated in glioma tumors and positively correlated with higher grade and poor survival. (antikoerper-online.de)
  • The cytoplasmic localization of YY1 was resistant to the nuclear export inhibitor leptomycin B. Evidence is presented indicating that nuclear import and export pathways were not adversely affected by vaccinia virus infection. (asm.org)
  • Larger proteins require active transport processes that involve peptide signal sequences in the transported proteins that are specific for different transport pathways. (asm.org)
  • We now demonstrate that in the importin α/β and transportin import pathways, efficient in vitro transport of large proteins, in contrast to smaller proteins, requires hydrolyzable GTP and the small GTPase Ran. (rupress.org)
  • The NPLOC4 - UFD1 - VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope (PubMed:10811609, PubMed:11740563, PubMed:11781570, PubMed:12411482, PubMed:12644454, PubMed:14636562). (rcsb.org)
  • LRRC42 protein contains two leucine-rich repeats (LRRs) which are widespread structural motifs comprising 20-30 amino acids with a characteristic repetitive sequence pattern rich in leucine residues. (spandidos-publications.com)
  • Previous work has shown that the transport of some small protein cargoes through the nuclear pore complex (NPC) can occur in vitro in the absence of nucleoside triphosphate hydrolysis. (rupress.org)
  • I use the antibody for DMP1 to pull down the protein. (protocol-online.org)
  • Antibody C3/1 recognized an oocyte nucleoplasmic protein with biochemical and biophysical properties similar to those of protein N1-N2. (biologists.org)
  • Over 280,000 products but you can't find the right antibody for your protein or application? (acris-antibodies.com)
  • If you cannot find the target and/or product is not available in our catalog, please click here to contact us and request the product or submit your request for custom elisa kit production , custom recombinant protein production or custom antibody production . (mybiosource.com)
  • Interaction with RAN BP1 induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins (PubMed:20485264). (rcsb.org)
  • Importin-β (Impβ) is a major transport receptor for Ran-dependent import of nuclear cargo. (rupress.org)
  • LRRC42, which was found to localize in the nucleus of mammalian cells, is likely to interact with and stabilize GATAD2B (GATA zinc finger domain-containing 2B) and MBD3 (Methyl-CpG-binding domain protein 3) proteins that could contribute to lung cancer cell proliferation partly through the regulation of p21Waf1/Cip1. (spandidos-publications.com)
  • isotonic (or protein extraction from fragile cells): 10 mM Tris HCl, pH 7.5, with 2 mM MgCl 2 , 3 mM CaCl 2 , 0.3 M Sucrose. (sigmaaldrich.com)
  • The nuclear extract is from ch10T1/2 cells. (protocol-online.org)
  • The nuclear lamina is an essential component of metazoan cells. (biologists.org)
  • This activity was detected in the cytoplasmic fraction of virus-infected cells but was predominantly in the nuclear fraction of uninfected cells. (asm.org)
  • Cytoplasmic and nuclear extracts were prepared as described ( 18 , 23 ). (pnas.org)
  • performed specialized proteomics on nuclear extracts from embryonic rat cortical neurons to identify hundreds of proteins that could be S-nitrosylated. (sciencemag.org)
  • We used S-nitrosothiol resin-assisted capture on NO donor-treated nuclear extracts from rat cortical neurons and identified 614 S-nitrosylated nuclear proteins. (sciencemag.org)
  • The following procedure describes the preparation of crude nuclear extracts using a syringe or a glass tissue homogenizer. (sigmaaldrich.com)
  • Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis. (acris-antibodies.com)
  • In addition, lysine motifs were necessary for promoting the S-nitrosylation of HDAC2 and methyl-CpG binding protein 3 (MBD3). (sciencemag.org)
  • Herein we report identification of LRRC42 as a potential therapeutic target and also provide evidence that LRRC42 could interact with GATAD2B (GATA zinc finger domain containing 2B) and MBD3 (Methyl-CpG-binding domain protein 3) proteins that are likely to play a significant role in human pulmonary carcinogenesis. (spandidos-publications.com)
  • Inhibition by Impβ results from depletion nuclear RanGTP, and, in support of this mechanism, expression of mRFP-Ran reversed the inhibition. (rupress.org)
  • On the nuclear side of the NPC, RanGTP acts to dissociate Impβ from the complex. (rupress.org)
  • When known, the sub-nuclear compartment where the protein was found is reported. (programmableweb.com)
  • Vaccinia virus replicates in the cytoplasmic compartment of the cell, raising questions as to whether vaccinia virus has access to nuclear proteins. (asm.org)
  • Due to the high conservation of protein sequences between mouse and human FAM76B, MAbs against hFAM76B were shown to react with mouse FAM76B (mFAM76B) specifically. (sigmaaldrich.com)
  • We propose that these proteins act as activators or repressors of genes responsible for mediating the various auxin responses. (pnas.org)
  • Association of lamins with the inner nuclear membrane is mediated by specific modifications in the CaaX motif at their C-termini. (biologists.org)
  • Poxviruses are unique among DNA genome viruses because their genome never crosses the nuclear membrane where nucleic acid synthesis otherwise occurs. (asm.org)
  • Implications for the synthesis of CaaX-containing proteins are discussed and the difference from intranuclear fibrous lamina annulate lamellae formations is emphasized. (biologists.org)
  • Together, our findings characterize S-nitrosylated nuclear proteins in neurons and identify S-nitrosylation motifs that may be shared with other targets of NO signaling. (sciencemag.org)
  • The initiation and termination of vaccinia virus postreplicative transcription have been reported to require cellular proteins, some of which are believed to be nuclear proteins. (asm.org)
  • Nevertheless, there is also evidence for participation of host cell proteins in vaccinia virus transcription. (asm.org)
  • During import the karyopherin heterodimer dissociates: karyopherin alpha and import substrates enter and accumulate in the nucleoplasm, whereas karyopherin beta accumulates at the nuclear pore complex. (nih.gov)