• Phosphorylation-resistant site mutations in PIF7 result in increased nuclear localization and shade-induced gene expression, and consequently augment hypocotyl elongation. (elifesciences.org)
  • Blocking the interaction between PIF7 and 14-3-3 proteins or reducing the expression of 14-3-3 proteins accelerates shade-induced nuclear localization and de-phosphorylation of PIF7, and enhances the shade phenotype. (elifesciences.org)
  • These studies have revealed that 14-3-3 proteins can interact with the phosphorylated forms of their client proteins in response to certain signals, and that this binding finalizes the signaling event by enabling a change in the subcellular localization, protein stability or intrinsic enzymatic activity of the client, or by promoting an association between the client and other proteins. (elifesciences.org)
  • Detailed prediction of protein sub-nuclear localization. (biomedcentral.com)
  • One such mechanism may be defective nuclear import of TDP-43 protein, as a disruption of its nuclear localization signal leads to mislocalization and aggregation of TDP-43 in the cytoplasm. (crick.ac.uk)
  • To identify the domains essential for virion incorporation and nuclear localization, site-directed mutations were introduced into the vpx gene of SIVsmPBj1.9 and functionally analyzed. (duke.edu)
  • and (iv) a mutation at E30 (predicted to disrupt an N-terminal alpha-helix) had no effect on either virion incorporation or nuclear localization of Vpx. (duke.edu)
  • Importantly, mutations in Vpx which impaired nuclear localization also reduced virus replication in macaque macrophages, suggesting an important role of the carboxyl terminus of Vpx in nuclear translocation of the viral preintegration complex. (duke.edu)
  • Taken together, these results indicate that virion incorporation and nuclear localization are encoded by two partially overlapping domains in the C-terminus of Vpx (aa 60 to 112). (duke.edu)
  • GFP-fused PfNapS fusion protein targeting indicates cellular localization of PfNapS in the parasite nucleus. (biomedcentral.com)
  • Erratum: Identification and characterization of nuclear and nucleolar localization signals in 58-kDa microspherule protein (MSP58). (bvsalud.org)
  • Mayya V, Lundgren DH, Hwang SI, Rezaul K, Wu L, Eng JK, Rodionov V, Han DK: Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. (hmdb.ca)
  • One of the largest transcription factor families is the nuclear receptor (NR) superfamily. (ku.edu)
  • The 1st identified mobile receptor because of this nuclear proteins was the receptor for advanced glycation end items (Trend), which mediates the relationships between advanced glycation end item (Age group)Cmodified proteins as well as the endothelium and additional cell types (7). (exposed-skin-care.net)
  • We previously identified two cytosolic proteins that reconstitute this step in a permeabilized cell assay: the 54/56-kD NLS receptor and p97. (rupress.org)
  • May be a nuclear hormone receptor coactivator. (lu.se)
  • A nuclear protein is a protein found in the cell nucleus. (wikipedia.org)
  • Proteins are transported inside the nucleus with the help of the nuclear pore complex, which acts a barrier between cytoplasm and nuclear membrane. (wikipedia.org)
  • This selectivity helps protect the DNA and is the job of machines that stud the envelope of the nucleus, called nuclear pore complexes. (sciencedaily.com)
  • Jan. 31, 2022 The nucleus is guarded by a highly secure door, the so-called nuclear pore, that controls the transport of substances from the cytoplasm to the cell nucleus and back. (sciencedaily.com)
  • The chemical reactions and pathways resulting in the breakdown of misfolded proteins via a mechanism in which the proteins are transported to the nucleus for ubiquitination, and then targeted to proteasomes for degradation. (yeastgenome.org)
  • in response to interferon treatment the protein kinase is induced severalfold in the cytoplasm whereas the level in the nucleus does not increase significantly. (pasteur.fr)
  • The cyclin D1 proteins primarily remains unpredictable until phospho-Pin1 in the nucleus isomerizes cyclin D1 to a well balanced conformation. (bioskinrevive.com)
  • Proteins found in the nucleus of a cell. (uams.edu)
  • Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus. (uams.edu)
  • Analyzing this domain in greater detail, we identified a 26-amino-acid (aa 60 to 85) fragment that was sufficient to mediate the transport of a heterologous protein (green fluorescent protein [GFP]) to the nucleus. (duke.edu)
  • The first suggests that disruption of the inner nuclear membrane and the nuclear lamina causes disorganization of nuclear chromatin and gene expression, while the second proposes that the mechanical strength of the cell nucleus is disrupted when the nuclear lamina is weakened leading to structural and signaling defects in mechanically stressed tissue such as muscle and heart. (medscape.com)
  • The most common mutations change single protein building blocks (amino acids) in the ATRX protein. (medlineplus.gov)
  • Other mutations insert or delete genetic material in the ATRX gene or alter how the gene's instructions are used to make the protein. (medlineplus.gov)
  • Mutations may destabilize the ATRX protein or affect its interactions with other proteins. (medlineplus.gov)
  • Argentaro A, Yang JC, Chapman L, Kowalczyk MS, Gibbons RJ, Higgs DR, Neuhaus D, Rhodes D. Structural consequences of disease-causing mutations in the ATRX-DNMT3-DNMT3L (ADD) domain of the chromatin-associated protein ATRX. (medlineplus.gov)
  • In 5 of 6 gene mutations that have been shown to cause EDMD, the affected protein is present in the LINC (linker of nucleoskeleton and cytoskeleton) complex. (medscape.com)
  • EDMD1 is caused by mutations in the EMD gene on the X chromosome that codes for the nuclear envelope protein emerin. (medscape.com)
  • New mutations have been found in the synaptic nuclear envelope protein 1 ( SYNE1 ) gene and in the synaptic nuclear envelope protein 2 ( SYNE2 ) gene in a few families, also termed Nesprin-1 and Nesprin-2, respectively. (medscape.com)
  • Lastly, mutations in the transmembrane protein 43 (TMEM43), also termed LUMA, which binds to emerin and SUN2, has also been reported to cause an EDMD phenotype in a few families. (medscape.com)
  • Whatever the true mechanism, the discovery of mutations in several different nuclear membrane proteins that cause similar diseases will likely eventually lead to a better understanding of nuclear membrane physiology and the pathophysiology of diseases caused by mutations in these proteins. (medscape.com)
  • Abcam: antibodies, proteins, kits. (abcam.com)
  • The appropriate species Alexa Fluor 488 and 568-conjugated antibodies were used as secondary antibodies and 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) was applied as a nuclear stain. (biomedcentral.com)
  • Evidence is presented that proteins recognized by the anti‐HMG antibodies also participate in Mar‐interactions. (nebraska.edu)
  • Thus, the majority of SR proteins and hnRNPs have the potential to regulate HPV16 L1 mRNA splicing. (lu.se)
  • Qualitative and quantitative approaches are undertaken to effectively integrate parameters from both techniques to shed new light onto how IDPs and their interactions with folded proteins regulate and enable various biological processes. (fu-berlin.de)
  • In order to explore the factors that regulate the nuclear import of TDP-43, we used a small interfering RNA library to silence 82 proteins involved in nuclear transport and found that knockdowns of karyopherin-beta1 and cellular apoptosis susceptibility protein resulted in marked cytoplasmic accumulation of TDP-43. (crick.ac.uk)
  • In addition to binding to RNA, nuclear factor 90 proteins form heterodimeric complexes that regulate GENETIC TRANSCRIPTION and may play a role in T-CELL activation. (ouhsc.edu)
  • Histone chaperones are proteins that regulate the interaction of histones with other proteins and DNA and also prevent the highly basic histones from forming inappropriate aggregates [ 2 , 3 ]. (biomedcentral.com)
  • Although the specific function of the ATRX protein is unknown, studies suggest that it helps regulate the activity (expression) of other genes through a process known as chromatin remodeling. (medlineplus.gov)
  • The ATRX protein appears to regulate the expression of two genes, HBA1 and HBA2 , that are necessary for the production of hemoglobin. (medlineplus.gov)
  • Zyagen provides high quality ready to use cytoplasmic protein of wide variety of normal healthy human tissues that might difficult to obtain because of size, anatomical complexity, or high protease activity. (delos.info)
  • Cytoplasmic protein is ready to immediate use for studying cytoplasmic soluble proteins. (delos.info)
  • Cytoplasmic protein is ideal for many downstream applications such as Western blotting, protein assays, enzyme-activity assays, gel electrophoresis, immunoprecipitation, protein-protein interaction analysis, and tissue specific expression identification. (delos.info)
  • The quality of cytoplasmic protein as indicated by the absence of smear (no degradation) and sharpness and resolution of the protein bands are verified by denatured SDS-PAGE with Coomassie blue staining. (delos.info)
  • Protein is also validated by Western Blot using specific cytoplasmic protein biomarker, GAPDH or beta-actin. (delos.info)
  • Nuclear extracts and cytoplasmic protein from RA-FLS treated with cytokines were analyzed. (biomedcentral.com)
  • ProteinExt ® Mammalian Nuclear and Cytoplasmic Protein Extraction Kit provides a fast and efficient method to extract nuclear and cytoplasmic proteins from mammalian cells and tissues. (transgenbiotech.com)
  • All functions of mitochondria depend on the interaction of nuclear and organellar genomes. (nih.gov)
  • Can function as a specific repressor of TGF-beta, activin, and BMP signaling through its interaction with the R-SMAD proteins. (hmdb.ca)
  • Through this interaction, mRNAs and their associative proteins form messenger ribonucleoprotein particles (mRNPs) that are actively transported along the cytoskeleton to intracellular destinations. (frontiersin.org)
  • The interaction of Ran with the guanine nucleotide exchange factor protein RCC1 was also studied by (31)P NMR spectroscopy. (mpg.de)
  • Histone H1 and nuclear actin are shown to be not only important components of the matrix, but to be involved in a highly efficient interaction with MAR‐sequences as well. (nebraska.edu)
  • All globular proteins contain large numbers of interaction, in particular, its dynamic aspects labile hydrogen atoms that can exchange with the (Daszkiewicz et al . (lu.se)
  • Immunodepletion of p97 renders the cytosol inactive for import and identifies at least three other cytosolic proteins that interact with p97. (rupress.org)
  • 8, 2020 Like wrenches made of Legos, SWI/SNF chromatin remodeling complexes tighten or loosen DNA in our cells to control how genes are turned on and made into proteins. (sciencedaily.com)
  • In order to collect information on nuclear coded mitochondrial proteins we developed MitoNuc and MitoAln, two related databases containing, respectively, detailed information on sequenced nuclear genes coding for mitochondrial proteins in Metazoa and yeast, and the multiple alignments of the relevant homologous protein coding regions. (nih.gov)
  • Other genes regulated by the ATRX protein have not been identified. (medlineplus.gov)
  • The research, performed by Vivien Nagy, a visiting graduate student, André Hoelz, a research associate, and colleagues in Rockefeller University's Laboratory of Cell Biology, uncovered the molecular structure of three interacting proteins that form the centerpiece of the Nup84 complex - an important structural component of the nuclear pore complex. (sciencedaily.com)
  • The Qproteome Nuclear Protein Kit is intended for molecular biology applications. (qiagen.com)
  • RA33 is a nuclear autoantigen with an apparent molecular mass of 33 kD. (jci.org)
  • In summary, these data strongly indicate that RA33 and A2 are the same protein, and thus identify on a molecular level a new autoantigen. (jci.org)
  • The team led by Dr. Kuo-Chiang Hsia (Institute of Molecular Biology, Academia Sinica) and Dr. Wei-Yi Chen (Institute of Biochemistry and Molecular Biology, College of Life Sciences, National Yang Ming Chiao Tung University) presents a cryo-EM structure of Kap114p, one of the Kap-βs, in complex with TATA box binding protein and reveals a non-canonical function beyond nuclear transport that modulates TBP-dependent transcription. (sinica.edu.tw)
  • My group exploits the complementary nature and distance dependence of nuclear magnetic resonance (NMR) and single molecule fluorescence, in particular single molecule Förster Resonance Energy Transfer (FRET), to study the conformational landscape and dynamics of IDPs at molecular resolution. (fu-berlin.de)
  • Emerin is a ubiquitous inner nuclear membraneprotein, presentin nearly all cell types, although its highest expression is in skeletal and cardiacmuscle.Emerin binds to many nuclear proteins, including several gene-regulatory proteins (eg, barrier-to-autointegration factor, germ cell-less, Btf), nesprins (proteins that act as molecular scaffolds), F-actin, and lamins. (medscape.com)
  • Funciona como andamio molecular para localizar y estabilizar estas proteínas en los sitios de recombinación homóloga. (bvsalud.org)
  • It functions as a molecular scaffold to localize and stabilize these proteins at homologous recombination sites. (bvsalud.org)
  • Progeria is caused by a sporadic mutation in the LMNA gene that codes for a protein (lamin A) that provides the molecular scaffolding of cell nuclei. (msdmanuals.com)
  • The import and export of proteins through the nuclear pore complex plays a fundamental role in gene regulation and other biological functions. (wikipedia.org)
  • They also describe competing interactions within the Nup84 complex and discuss the possibility of binding promiscuity as a common feature in the nuclear pore complex. (sciencedaily.com)
  • These findings suggest that more than one assemblage of its elements may be necessary for the function of the nuclear pore complex - to import and export macromolecules including ribosomes and messenger RNA. (sciencedaily.com)
  • Now that we realize that promiscuity may be a major factor in the nuclear pore complex - that the nucleoporins have different ways of interacting with each other - the complex is no longer just a three-dimensional jigsaw puzzle," Hoelz says. (sciencedaily.com)
  • p97 is extracted from nuclear envelopes under the same conditions as the O-glycosylated nucleoporins indicating a tight association with the pore complex. (rupress.org)
  • The antibody inhibits import in a permeabilized cell assay but does not affect binding of karyophiles to the nuclear pore complex. (rupress.org)
  • The article is titled "Nuclear Accumulation of CHMP7 Initiates Nuclear Pore Complex Injury and Subsequent TDP-43 Dysfunction in Sporadic and Familial ALS. (bioquicknews.com)
  • Solid-state NMR (ssNMR) is a versatile technique that can provide high-resolution (sub-angstrom) structural data for integral membrane proteins embedded in native and model membrane environments. (nih.gov)
  • However, the techniques are now being applied to large, polytopic membrane proteins including receptors, ion channels, and porins. (nih.gov)
  • This review describes the spectroscopic experiments and data analysis methods (including assignment) used to generate high-resolution structural data for membrane proteins. (nih.gov)
  • Nucleosome assembly proteins (NAPs) are histone chaperones that are crucial for the shuttling and incorporation of histones into nucleosomes. (biomedcentral.com)
  • In addition to playing an important role in histone exchange during nuclear processes, histone chaperones function in nucleocytoplasmic shuttling of histones, in histone storage, in nucleosome assembly and they act as a link between chromatin remodeling factors and histones [ 2 , 3 ]. (biomedcentral.com)
  • They bind to structural components (emerin, nesprin), chromatin components (histone), signal transduction molecules (protein kinase C), and several gene regulatory molecules. (medscape.com)
  • Nuclear location sequence-mediated binding of karyophilic proteins to the nuclear pore complexes is one of the earliest steps in nuclear protein import. (rupress.org)
  • This process requires the involvement of nuclear transport receptors, collectively termed karyopherin-ß (Kap-ß) in yeast, and various regulatory factors. (sinica.edu.tw)
  • We show that quantitative internuclear (15)N-(13)C distances can be obtained in sufficient quantity to determine a complete, high-resolution structure of a moderately sized protein by magic-angle spinning solid-state NMR spectroscopy. (rcsb.org)
  • Researchers have also shown that one member of the three-protein structure interacts promiscuously with two nucleoporins as do other proteins in the nuclear pore, supporting a model of a flexible complex that can rearrange itself into different formations. (sciencedaily.com)
  • Pan D, Estevez-Salmeron LD, Stroschein SL, Zhu X, He J, Zhou S, Luo K: The integral inner nuclear membrane protein MAN1 physically interacts with the R-Smad proteins to repress signaling by the transforming growth factor-{beta} superfamily of cytokines. (hmdb.ca)
  • PIF7 interacts with 14-3-3 proteins. (elifesciences.org)
  • Isoform 3 interacts with HCV core protein. (lu.se)
  • Sequence analysis of five tryptic peptides revealed that their sequences matched corresponding sequences of the A2 protein of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. (jci.org)
  • Phosphorylated Pin1 protein had been digested with trypsin and peptides had been examined by 2D electrophoresis as referred to (30) ( SI Components and Strategies ). (bioskinrevive.com)
  • We then describe the evolution of genomic organisation across lineages, how this process has been shaped by interactions with the nuclear genome and population genetics dynamics. (nature.com)
  • These studies demonstrate a phosphorylation-dependent translocation of PIF7 when plants are in shade and a novel mechanism involving 14-3-3 proteins, mediated by the retention of PIF7 in the cytoplasm that suppresses the shade response. (elifesciences.org)
  • A monoclonal antibody to p97 localizes the protein to the cytoplasm and the nuclear envelope. (rupress.org)
  • The levels and subcellular distribution of the interferon-inducible double-stranded RNA-dependent protein kinase PKR have been measured in human Daudi cells and stably transfected mouse NIH 3T3 cells expressing the human protein kinase. (pasteur.fr)
  • mixed-lineage kinase 3 (MLK3) a MAP3K family member phosphorylates Pin1 on a Ser138 site to increase its catalytic activity and nuclear translocation. (bioskinrevive.com)
  • Lin F, Morrison JM, Wu W, Worman HJ: MAN1, an integral protein of the inner nuclear membrane, binds Smad2 and Smad3 and antagonizes transforming growth factor-beta signaling. (hmdb.ca)
  • trnp: A conserved mammalian gene encoding a nuclear protein that accelerates cell-cycle progression. (nih.gov)
  • The Qproteome Nuclear Protein Kit is designed for specific enrichment of nuclear proteins from cultured mammalian cells. (qiagen.com)
  • Purification and partial sequencing of the nuclear autoantigen RA33 shows that it is indistinguishable from the A2 protein of the heterogeneous nuclear ribonucleoprotein complex. (jci.org)
  • After washing, an extraction buffer is added to the nuclei and nucleic acid binding proteins dissociate from DNA and RNA. (qiagen.com)
  • The Qproteome Nuclear Protein Kit delivers a nucleic acid binding protein fraction suitable for a wide range of activity assays. (qiagen.com)
  • NX1 extraction buffer (Blank) or 10 µg nucleic acid binding protein fraction was added, washed, and detected colorimetrically in an ELISA procedure using a transcription-factor specific antibody. (qiagen.com)
  • [ 1 ] Even though these proteins are ubiquitously expressed, disease manifestations are tissue specific for as yet unclear reasons. (medscape.com)
  • A high-salt buffer allows dissociation of nuclear binding proteins (such as transcription factors) and their removal by diffusion from the nuclei. (qiagen.com)
  • A potential submicrosecond for solvent-accessible carboxyl complication in such studies is the exchange of groups to years for deeply buried amide hydrogen hydrogen nuclei between water and protein. (lu.se)
  • This complex includes nuclear membrane integral and associated proteins including emerin, lamin A/C, SUN1, SUN2, nesprin-1, and nesprin-2 that are proposed to form a mechanical link between the nucleoskeleton and cytoskeleton. (medscape.com)
  • CBP20 is a nuclear cap-binding protein that forms a heterodimeric complex with ABH1 (AT2G13540) and is likely to participate in RNA metabolism. (phytoab.com)
  • Nuclear protein peptidyl-prolyl isomerase Pin1-mediated prolyl isomerization can be an essential and novel regulatory mechanism for protein phosphorylation. (bioskinrevive.com)
  • This soluble fraction is separated from the nuclear pellet by centrifugation. (qiagen.com)
  • Nuclear Proteins" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (uams.edu)
  • abstract = "The recent discovery of DNA sequences responsible for the specific attachment of chromosomal DNA to the nuclear skeleton (MARs/SARs) was an important step towards our understanding of the functional and structural organization of eukaryotic chromatin [Mirkovitch et al. (nebraska.edu)
  • Chromatin is highly dynamic, a characteristic that is vital in regulating nuclear processes such as transcription and replication which require access to DNA. (biomedcentral.com)
  • Chromatin is the complex of DNA and protein that packages DNA into chromosomes. (medlineplus.gov)
  • Identification of a nuclear protein, LRRC42, involved in lung carcinogenesis. (uchicago.edu)
  • Scholars@Duke publication: Functional analysis of the simian immunodeficiency virus Vpx protein: identification of packaging determinants and a novel nuclear targeting domain. (duke.edu)
  • The identification of a novel 26-amino-acid nuclear targeting domain provides a new tool to investigate the nuclear import of the HIV-2/SIV preintegration complex. (duke.edu)
  • Thus, it is important to understand the fundamental biological processes of P. falciparum , which may provide avenues for the identification of new protein targets for development of new anti-malarials. (biomedcentral.com)
  • Here, applying a different approach, we were able to "visualize" some of the skeletal proteins recognizing and specifically binding MAR‐sequences. (nebraska.edu)
  • Here we report a class of mRNA granules in human neuronal processes that are enriched in the nuclear cap-binding protein complex (CBC) and exon junction complex (EJC) core components, Y14 and eIF4AIII. (frontiersin.org)
  • The extracted proteins are suitable for a variety of downstream applications, including SDS-PAGE, Western blot, ELISA, enzyme-activity assays, immunoprecipitation and transcription factor activity analysis. (transgenbiotech.com)
  • This pD dependence is due to labile protein deuterons in acidic side-chains and surface peptide groups, which exchange rapidly with water deuterons. (lu.se)
  • cDNA cloning of p97 shows that it is a unique protein containing 23 cysteine residues. (rupress.org)
  • Most heritable information in eukaryotic cells is encoded in the nuclear genome, with inheritance patterns following classic Mendelian segregation. (nature.com)
  • Two-dimensional isoelectric focusing analysis of PKR in whole cell extracts reveals the presence of multiple forms with different pI values whereas similar analysis of the nuclear fraction indicates only one predominant species with a relatively basic pI. (pasteur.fr)
  • (B, C) Western blotting for C/EBPβ protein consisting of 38 kD liver-enriched activator protein (LAP*), 36 kD LAP and 20 kD liver-enriched inhibitory protein (LIP) (B) Experiments with whole extracts of primary cultured passage-1 RA fibroblast-like synoviocytes (RA-FLS) from three patients (RA1, RA2 and RA3) and normal human fibroblast-like synoviocytes (HFLS). (biomedcentral.com)
  • Inhibitory effect on activator protein-1, nuclear factor-kappaB, and cell transformation by extracts of strawberries (Fragaria x ananassa Duch. (cdc.gov)
  • Deleterious, protein-altering variants in the transcriptional coregulator ZMYM3 in 27 individuals with a neurodevelopmental delay phenotype. (uams.edu)
  • Tissues are freshly dissected and homogenized in modified protein lysis buffer supplemented with a cocktail of 7 protease inhibitors to minimize proteolysis. (delos.info)
  • One of these proteins is the fibroblast activation protein (FAP), a type II transmembrane serine protease with both dipeptidyl peptidase activity and endopeptidase activity ( 2 - 4 ). (snmjournals.org)
  • These extremely dynamic proteins are highly flexible and easily adaptable to different binding partners, making them important players in many biological processes, often with vital regulatory functions. (fu-berlin.de)
  • antioxidant enzymes on tetradecanoylphorbol-13-acetate (TPA) or ultraviolet-B (UVB) induced activator protein-1 (AP-1) and nuclear factor-kappaB (NF-kappaB) were studied. (cdc.gov)
  • Upon activation of MLK3 by known agonists ceramide and TNFα (27) or various other unidentified agonists MLK3 could phosphorylate Pin1 in the S138 site (Fig. S7) and promote its nuclear translocation. (bioskinrevive.com)
  • This review will highlight new discoveries regarding the mechanistic role of NRs and their associated protein cofactors in regulating gene activation and in the progression of cancer, diabetes, and obesity. (ku.edu)
  • We propose that cellular apoptosis susceptibility protein associated defective nuclear transport may play a mechanistic role in the pathogenesis of the TDP-43 positive frontotemporal lobar degeneration. (crick.ac.uk)