• The expression of cyclin-dependent kinase inhibitors was profoundly affected with early activation and then repression of p21 cip1/waf1 and persistent activation of both p27 kip1 and p57 kip2 , whereas genes involved in cell survival and proliferation were suppressed. (aacrjournals.org)
  • In humans, the CRYAA gene encodes a 173 amino acid residue protein by single copy genes located on chromosome 21. (molvis.org)
  • We find that nuclear organization has the greatest impact, and that genes encoding for physically interacting proteins specifically tend to covary, suggesting importance for protein complex formation. (nih.gov)
  • Our results lend support to the concept of post-transcriptional RNA operons, but we further present evidence that nuclear proximity of genes may provide substantial functional regulation in mammalian single cells. (nih.gov)
  • 8- 10 In attempts to identify new breast and ovarian cancer susceptibility genes, much research has focused on BRCA1 associated proteins. (bmj.com)
  • Finally, transcription factors within the nucleus must initiate the transcription of genes involved in cell proliferation. (britannica.com)
  • However, the cell cycle and its checkpoint systems can be sabotaged by defective proteins or genes that cause malignant transformation of the cell, which can lead to cancer . (britannica.com)
  • Myb-Related Protein B (MYBL2), a member of the MYB family of transcription factor genes, is a nuclear protein involved in cell cycle progression. (avivasysbio.com)
  • It has been shown to activate the cell division cycle 2, cyclin D1, and insulin-like growth factor-binding protein 5 genes. (avivasysbio.com)
  • Analyzing 5′-upstream non-protein-encoding regions of the human mitochondrial function-associated genes, we speculate that mitochondrial functions could be recovered or improved at a transcriptional level. (intechopen.com)
  • Numerous genes transcribed in oocytes are involved in multiple aspects of cell maintenance and protection, including metabolism, signal perception and transduction, RNA processing, cell cycle, defense against pathogens and DNA damage. (biomedcentral.com)
  • Inactivation of tumor suppressor genes, whose products normally provide negative control of cell proliferation, contributes to malignant transformation in various cell types. (medscape.com)
  • Like Rb protein, many of the proteins encoded by tumor suppressor genes act at specific points in the cell cycle. (medscape.com)
  • TP53 activates the expression of genes involved in apoptosis, cell cycle regulation (p21), and MDM2. (medscape.com)
  • Another important class of tumor suppressor genes involved in cell cycle control and in the generation of human cancers is the cyclin-dependent kinase (CDK) inhibitors. (medscape.com)
  • It represses the expression of genes involved in the tricarboxylic acid cycle while promoting expression of genes involved in electron transfer chain. (lu.se)
  • Below is a non-exhaustive list of in-house infrastructures that are categorized into three overarching themes: bio-imaging, proteins, genes & cells and other resources. (lu.se)
  • Below you can see some examples of the infrastructure for research on genes and cells, available for researchers at Lund University. (lu.se)
  • In addition to infrastructures for bioimaging, protein and genes & cells, we also provide other resources e.g., databases, networks and specialized labs. (lu.se)
  • The encoded protein contains an NIRF_N domain, a PHD finger, a set- and ring-associated (SRA) domain, and a RING finger domain and several of these domains have been shown to be essential for the regulation of cell proliferation. (nih.gov)
  • Using proteomics approaches, we have discovered the interaction of EBNA1 with host proteins that control cell proliferation and apoptosis (USP7 and CK2) and with host nucleosome associated proteins that regulate DNA replication and transcription through histone modifications. (utoronto.ca)
  • The purpose of this study was to determine the acute and long-term effects of mitomycin C (MMC) on quiescent rabbit corneal keratocytes regarding cell proliferation, myofibroblast differentiation and DNA repair. (molvis.org)
  • MMC significantly blocked TGFβ-induced cell proliferation and myofibroblast differentiation in cultured quiescent keratocytes and altered the transcriptional regulation of macrophage chemotactic protein-1 (MCP-1) and alpha smooth muscle actin (αSMA). (molvis.org)
  • Calcium channel blockers verapamil, nifedipine, diltiazem and isradipine arrested basic fibroblast growth factor induced human umbilical arterial endothelial cell proliferation. (cancerprev.org)
  • Our study demonstrates that survivin and APE1/Ref-1 are significantly higher in human prostate cancer specimens compared to noncancerous controls and that APE1/Ref-1 redox-specific inhibition with small molecule inhibitor, APX3330 and a second-generation inhibitor, APX2009, decreases prostate cancer cell proliferation and induces cell cycle arrest. (oncotarget.com)
  • Загальні відомості про плазмоклітинні захворювання Plasma cell disorders are a diverse group of disorders of unknown etiology characterized by Disproportionate proliferation of a single clone of B cells Presence of a structurally and electrophoretically. (msdmanuals.com)
  • However, potentially erroneous characterization of cytotoxicity and cell proliferation can arise if verification of baseline interaction of test article with method employed is neglected. (cdc.gov)
  • Non-tumorigenic Beas-2B cells were treated with graded concentrations of benzo[a]pyrene (B[a]P) for 24 and 48h prior to cytotoxicity and proliferation assessment with commonly used MTT, MTS, WST1, and Alamar Blue assays. (cdc.gov)
  • Epidemiologic, animal, and laboratory studies suggest that 5-amino-salicylic acid (5-ASA) protects from the development of CRC by altering cell cycle progression and by inducing apoptosis. (nih.gov)
  • In addition, we found that EBNA1 disrupts the host PML nuclear bodies required for apoptosis and DNA repair, thereby promoting cell survival with DNA damage. (utoronto.ca)
  • Unlike apoptosis, senescence is a state of which cells are still alive and metabolically active. (frontiersin.org)
  • We show here that expression of EBF3 resulted in cell cycle arrest and apoptosis. (aacrjournals.org)
  • If DNA damage or abnormalities in spindle formation are detected at these checkpoints, the cell is forced to undergo programmed cell death, or apoptosis . (britannica.com)
  • For example, mutations in a protein called p53 , which normally detects abnormalities in DNA at the G1 checkpoint, can enable cancer-causing mutations to bypass this checkpoint and allow the cell to escape apoptosis. (britannica.com)
  • Resveratrol could play a toxic role through inducing apoptosis of the cancer cell in a time- and concentration-dependent manner. (mdpi.com)
  • A key feature of prostate cancer progression is the induction and activation of survival proteins, including the Inhibitor of Apoptosis (IAP) family member survivin. (oncotarget.com)
  • The TP53 gene is also capable of stimulating apoptosis of cells containing damaged DNA. (medscape.com)
  • p53 is a transcription factor that participates in cell cycle checkpoint processes and apoptosis. (lu.se)
  • Role of PDL-1 in NSCLC chemoresistance was assessed using CRISPR/Cas9 knockdown and various functional assays including MTT, cell invasion, sphere formation, and cell apoptosis. (cdc.gov)
  • Mechanistically, an upregulation of PDL-1 in chemotherapy-treated CAFs led to an increase in hepatocyte growth factor (HGF) secretion, which stimulates cancer progression, cell invasion, and stemness of lung cancer cells, while inhibiting apoptosis. (cdc.gov)
  • Our previous results indicate that 5-ASA improves replication fidelity in colorectal cells, an effect that is active in reducing mutations. (nih.gov)
  • MCM2 (Mini chromosome maintenance protein 2) is involved in regulating DNA replication. (thermofisher.com)
  • MCM2 (also called CDCL1, mitotin and BM28), is a human nuclear protein that is crucial in the cell cycle, being involved in the onset of DNA replication and cell division. (thermofisher.com)
  • MMC induces DNA damage to quiescent corneal keratocytes, which remains unrepaired, resulting in abnormal cell replication and gene transcription that leads to long-term effects on corneal repair. (molvis.org)
  • These data indicate that the effect of MMC on corneal scarring and haze is related to the generation of DNA ICLs leading to defective cell replication and gene expression. (molvis.org)
  • Malaria parasites replicate inside the cells of their human host via 'schizogony', which is fundamentally different from conventional binary fission - the replication mode used by most cells from human cells to yeast cells. (europa.eu)
  • In schizogony a single parasite first generates many nuclei via independent, asynchronous rounds of genome replication, prior to cytokinesis which is the physical division of the cell. (europa.eu)
  • Thus, schizogony challenges some basic paradigms about DNA replication control, while gametogenesis demands a speed of DNA replication and cell division that is unprecedented in eukaryotic gametogenesis. (europa.eu)
  • This project is elucidating the spatio-temporal dynamics of DNA replication in these contrasting cell cycles. (europa.eu)
  • Aim 1: detailed characterisation, at the whole-cell level, of the asynchronous genome replication that occurs in schizogony and gametogenesis. (europa.eu)
  • Mechanisms for maintaining genetic information during cell division and the generation of genetic variation: replication, mitosis, meiosis, recombination. (lu.se)
  • We show that TMEM53 acts as an inhibitor of BMP-SMAD signaling by preventing SMAD accumulation in the cell nucleus and that its deficiency enhances osteogenic differentiation by overactivating the BMP signaling pathway. (nature.com)
  • Single-cell RNA sequencing studies on gene co-expression patterns could yield important regulatory and functional insights, but have so far been limited by the confounding effects of differentiation and cell cycle. (nih.gov)
  • Cell Growth and Differentiation. (uky.edu)
  • Johansen studies processes critical for normal cell differentiation and development, including how the active regions of the genome are protected from gene silencing and how nuclear proteins contribute to mitotic spindle organization and function during cell division. (iastate.edu)
  • Microarray analyses detected a suite of master developmental regulators that control differentiation and maintenance of diverse cell lineages. (biomedcentral.com)
  • This gene encodes a nuclear protein which is involved in cell-cycle regulation. (nih.gov)
  • Here, we discover a previously unknown type of SBD in four independent families caused by bi-allelic loss-of-function pathogenic variants in TMEM53 , which encodes a nuclear envelope transmembrane protein. (nature.com)
  • In this study, we discover a previously unknown type of SBD and identify its causal gene, TMEM53 , which encodes nuclear envelope transmembrane (NET) protein 53 (TMEM53, also known as NET4). (nature.com)
  • ALMS1 encodes a ~ 0.5 megadalton protein that localises to the base of centrioles. (springer.com)
  • For example, the TP53 gene, located on chromosome 17, encodes a 53-kd nuclear protein that functions as a cell cycle checkpoint. (medscape.com)
  • This locus, however, also encodes a protein from an alternative reading frame, designated p19ARF. (medscape.com)
  • In this study, we hypothesized that 5-ASA restrains cell cycle progression by activating checkpoint pathways in colorectal cell lines, which would prevent tumor development and improve genomic stability. (nih.gov)
  • We found that 5-ASA at concentrations between 10 and 40 mmol/L affects cell cycle progression by inducing cells to accumulate in the S phase. (nih.gov)
  • These studies include identifying proteins that affect PML nuclear bodies, cell cycle progression and DNA damage responses. (utoronto.ca)
  • It helps in deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) and gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. (novusbio.com)
  • The CDK4-cyclinD complex normally phosphorylates the retinoblastoma protein (Rb protein), leading to release of the E2F transcription factor and cell cycle progression. (medscape.com)
  • A nuclear protein, HDAC-9 belongs to the histone deacetylase family with several isoforms and is inhibited by Trichostatin A (TSA) and suberoylanilide hydroxamic acid. (novusbio.com)
  • Any process that stops, prevents, or reduces the frequency, rate or extent of the movement of proteins from the cytoplasm into the nucleus, and that occurs as a response to the mitotic cell cycle spindle assembly checkpoint. (yeastgenome.org)
  • In non-synchronized cell populations, extraction from chromatin necessitated 300 mM NaCl whereas 150 mM was sufficient in mitotic cells. (wikigenes.org)
  • system_update_alt데이터시트system_update_alt안전 데이터시트Overview 개요Product Name:Human SPAG5(sperm associated antigen 5) ELISA KitProduct Code:HUFI03067Size:96 AssaysAlias:Astrin, Deepest, Mitotic spindle-associated protein p126, MAP126Detection method:Sandwich. (assaygenie.kr)
  • Analyses of the molecular pathophysiology using the primary cells from the Tmem53 -/- mice and the TMEM53 knock-out cell lines indicates that TMEM53 inhibits BMP signaling in osteoblast lineage cells by blocking cytoplasm-nucleus translocation of BMP2-activated Smad proteins. (nature.com)
  • Surprisingly, despite anticipated defects in DNA binding or RAD51-mediated DNA strand exchange, the BRCA2 R3052W protein mislocalizes to the cytoplasm precluding its ability to perform any DNA repair functions. (frontiersin.org)
  • In Xp11.2 translocation-associated renal cell carcinoma (RCC), cells typically have voluminous cytoplasm, which can range from eosinophilic and granular to clear. (medscape.com)
  • at least focally, tumor cells often have abundant clear cytoplasm, mimicking clear cell RCC. (medscape.com)
  • In Xp11.2 translocation-associated renal cell carcinoma, tumor cells with abundant eosinophilic cytoplasm and high nuclear grade are often arranged in large nests with a delicate, intervening vascular stroma. (medscape.com)
  • The cyclin D1 proteins primarily remains unpredictable until phospho-Pin1 in the nucleus isomerizes cyclin D1 to a well balanced conformation. (bioskinrevive.com)
  • 25 BARD1 also regulates the subcellular localisation of BRCA1, both by translocating BRCA1 into the nucleus and by inhibiting its nuclear export. (bmj.com)
  • For a stimulatory signal to reach the nucleus and "turn on" cell division, four main steps must occur. (britannica.com)
  • Third, this activation must stimulate a signal to be transmitted, or transduced, from the receptor at the cell surface to the nucleus within the cell. (britannica.com)
  • Importins, proteins that transport macromolecules into the nucleus, interact with cargo and regulatory molecules at different times in the transport cycle. (nature.com)
  • Proteins involved in the ATM-and-Rad3-related kinase (ATR)-dependent S-phase checkpoint response (Chk1 and Rad17) were also phosphorylated but not ataxia telengectasia mutated kinase. (nih.gov)
  • mixed-lineage kinase 3 (MLK3) a MAP3K family member phosphorylates Pin1 on a Ser138 site to increase its catalytic activity and nuclear translocation. (bioskinrevive.com)
  • Glc7p functions in opposition to key spindle assembly checkpoint protein Aurora kinase (Ipl1p). (yeastgenome.org)
  • The encoded protein is phosphorylated by cyclin A/cyclin-dependent kinase 2 during the S-phase of the cell cycle and possesses both activator and repressor activities. (avivasysbio.com)
  • Phosphorylated Pin1 protein had been digested with trypsin and peptides had been examined by 2D electrophoresis as referred to (30) ( SI Components and Strategies ). (bioskinrevive.com)
  • Our data demonstrate that 5-ASA causes cells to reversibly accumulate in S phase and activate an ATR-dependent checkpoint. (nih.gov)
  • 6, 7 BRCA1 interacts with a variety of proteins and is involved in multiple cellular processes including DNA repair, transcription, and checkpoint control. (bmj.com)
  • Cells use special proteins and checkpoint signaling systems to ensure that the cell cycle progresses properly. (britannica.com)
  • Macrophages are essential inflammatory cells which regulate the features of immune reactions within tumors. (cancerindex.org)
  • Mechanisms that regulate development from single cell to multicellular organisms. (lu.se)
  • Tumor-infiltrated macrophages were isolated from human GC tissues using magnetic beads, gene transcription was determined by real-time PCR, protein expression was monitored using western blots, metabolites were determined using HPLC, and transcriptional regulation was analyzed by the luciferase-based reporter gene system. (cancerindex.org)
  • We provide the first evidence that miRNAs naturally induce transcriptome-wide covariations and compare the relative importance of nuclear organization, transcriptional and post-transcriptional regulation in defining covariations. (nih.gov)
  • BARD1, unlike BRCA1, also contains a centrally located sequence of three ankyrin repeats 11 that are found in many proteins involved in transcriptional regulation. (bmj.com)
  • The active full-length CRYAA protein corresponding to amino acids 1-173 of CRYAA was recombined. (molvis.org)
  • Denticleless protein homolog is a protein that in humans is encoded by the DTL gene. (wikipedia.org)
  • Ki-67 has been shown to interact with the DNA-bound protein chromobox protein homolog 3 (CBX3) (heterochromatin). (thermofisher.com)
  • In studies of tumor cells, the "Ki-67 labeling index" refers to the number of Ki-67 positive cells within the population and this is used to predict outcome of particular cancer types. (thermofisher.com)
  • Silencing of the EBF3 locus was observed in brain, colorectal, breast, liver, and bone tumor cell lines and its reactivation was achieved on treatment with 5-aza-2′-deoxycytidine and trichostatin A in a significant portion of these tumor cells. (aacrjournals.org)
  • elicits antiproliferative and proapoptotic activities in vitro in solid and hematologic tumor cells. (medscape.com)
  • These changes can be inherited and are, therefore, found in every cell, but more often, they are somatically acquired and restricted to tumor cells. (medscape.com)
  • within a given tumor, cells may be variably arranged in broad sheets, nests, trabeculae, true papillae, or pseudopapillae (see the first 3 images below). (medscape.com)
  • The nests of tumor cells in Xp11.2 translocation-associated renal cell carcinomas may become centrally discohesive, giving rise to a pseudoalveolar growth pattern. (medscape.com)
  • Timecourses have been conducted to quantify the number and type of replicating nuclei, together with other cell-biological features, in P. falciparum parasites across the course of both erythrocytic schizogony and gametogenesis. (europa.eu)
  • CnHsf3 acts in nuclei and mitochondria, and nuclear- and mitochondrial-targeting signals are required for its organelle-specific functions. (lu.se)
  • In contrast lytic infection induces cell cycle arrest and leads to amplification of the viral genomes for virion production. (utoronto.ca)
  • Aging kidney and CKD share many common characteristic features with increased cellular senescence, a conserved program characterized by an irreversible cell cycle arrest with altered transcriptome and secretome. (frontiersin.org)
  • Cellular senescence is characterized by an irreversible and permanent cell cycle arrest coupled with altered transcriptome and secretome. (frontiersin.org)
  • Interest in ALMS1 is heightened by the recent discovery of its involvement in neonatal cardiomyocyte cell cycle arrest, a process with potential relevance to regenerative medicine. (springer.com)
  • Mechanisms regulating cardiomyocyte cell cycle arrest are of great interest partly because reversing this process could provide a way to stimulate cardiac regeneration after injury [ 17 ]. (springer.com)
  • Activation of p21 or p16 therefore causes cell cycle arrest. (medscape.com)
  • The p19ARF protein, which is encoded by the same locus as p16, also leads to cell cycle arrest by inhibiting the ability of MDM2 to inactivate TP53. (medscape.com)
  • Because APE1/Ref-1 is expressed and elevated in prostate cancer, we sought to characterize APE1/Ref-1 expression and activity in human prostate cancer cell lines and determine the effect of selective reduction-oxidation (redox) function inhibition on prostate cancer cells in vitro and in vivo . (oncotarget.com)
  • Thus, the principle of protein or peptide haptenation could be used in in vitro assays to predict the sensitization potential of a new chemical entity. (cdc.gov)
  • CRYAA interacted with these proteins to maintain their solubility and decrease the accumulation of denatured target proteins. (molvis.org)
  • Mitochondrial quality control prevents accumulation of intramitochondrial-derived reactive oxygen species (mtROS), thereby protecting cells against DNA damage, genome instability, and programmed cell death. (lu.se)
  • We aimed to determine the signaling pathway regulating cell metabolism and the mechanism related to the regulation of human tumor-associated macrophages (TAMs) in gastric cancer (GC). (cancerindex.org)
  • Planar Cell Polarity (PCP), the polarization within the plane of an epithelium, is perpendicular to apical-basal polarity and established by the non-canonical Wnt/Fz-PCP signaling pathway. (uni-wuerzburg.de)
  • Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol B: One step protocol for intracellular (nuclear) proteins" located at Flow Protocols . (thermofisher.com)
  • Vincristine inhibits cellular mitosis by inhibition of intracellular tubulin function, binding to microtubules, and synthesis of spindle proteins in the S phase. (medscape.com)
  • The three germ layers and the body plan of the mature organism are established during gastrulation, and the period is characterized by extensive cell movements and intracellular communications [ 21 , 22 ]. (biomedcentral.com)
  • Zinc, an essential micronutrient, is required by many enzymes involved in gene expression and, therefore, is vital in maintaining DNA, RNA, and protein synthesis in cells. (usda.gov)
  • DNA and Cell Biology. (umn.edu)
  • Molecular Cell Biology Research Communications. (umn.edu)
  • The course is an optional second-cycle course for a degree of Bachelor or Master of Science in Biology and Molecular Biology. (lu.se)
  • Gene regulation in developmental biology and the cell cycle. (lu.se)
  • Our facilities provide the opportunity to study protein structure, molecular probes and drug design, system biology and molecular interactions in cells and tissues. (lu.se)
  • The BRCA2 R3052W mutated protein exacerbates genome instability, is unable to rescue homology-directed repair, and fails to complement cell survival following exposure to PARP inhibitors and crosslinking drugs. (frontiersin.org)
  • However, the genome can also be copied extremely rapidly during the sexual cycle, which occurs in the malaria-transmitting mosquito. (europa.eu)
  • The oocyte is loaded with maternal mRNAs and proteins that control the cell maintenance and fate and the formation of the body plan prior to the onset of zygotic genome expression [ 3 , 4 ]. (biomedcentral.com)
  • In latency EBV genomes are maintained as circular nuclear episomes that replicate once-per-cell cycle and are partitioned to the daughter cells during mitosis. (utoronto.ca)
  • Ki-67 is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells (G0). (thermofisher.com)
  • BRCA2 nuclear localization and export sequences are listed. (frontiersin.org)
  • Figure 1: Nuclear import of proteins containing different basic nuclear-localization sequences (NLSs) by importin-β. (nature.com)
  • Studies in the Frappier laboratory involve a variety of methodologies, including proteomics approaches for protein interactions and host cell manipulations, functional assays in human cells, immunofluorescence microscopy, RNA interference, protein biochemistry and protein structural studies. (utoronto.ca)
  • To identify proteins interacting with alpha A-crystallin (CRYAA) and to investigate the potential role that these protein interactions play in the function of CRYAA using a human proteome (HuProt) microarray. (molvis.org)
  • A HuProt microarray composed of 17,225 human full-length proteins with N-terminal glutathione S-transferase (GST) tags was used to identify protein-protein interactions. (molvis.org)
  • The protein-protein interactions may help CRYAA carry out multifaceted functions. (molvis.org)
  • To understand the functions of CRYAA and how these are regulated, protein-protein interactions need to be investigated. (molvis.org)
  • No yeast two-hybrid interactions found for this protein. (yeastrc.org)
  • Transcripts found in unfertilized oocytes also encoded a large number of proteins implicated in cell adherence, tight junction and focal adhesion, suggesting high complexity in terms of structure and cellular interactions in embryos prior to midblastula transition (MBT). (biomedcentral.com)
  • 1991), none of the the structural details of the heterogeneous protein surface and experimental studies mentioned so far could provide un- takes into account a variety of short-range interactions, direct ambiguous information about oligomer structure. (lu.se)
  • Cells were then surface-stained with CD45R (B220) Monoclonal Antibody, FITC (Product # 11-0452-82) and stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol, with 1.0 µg of Ki-67 Monoclonal Antibody, Brilliant Ultra Violet 615. (thermofisher.com)
  • Description: The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300 kDa nuclear protein. (thermofisher.com)
  • A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. (thermofisher.com)
  • Western Blot: HDAC9 Antibody [NBP2-03993] - Analysis of HDAC-9 in human HeLa cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) and mouse RAW cell lysate using this antibody. (novusbio.com)
  • Antibody- and T-cell-based approaches to targeting of B-cell maturation antigen have shown efficacy. (msdmanuals.com)
  • Scope includes mutations and abnormal protein expression. (cancerindex.org)
  • A diverse group of proteins whose genetic MUTATIONS have been associated with the chromosomal instability syndrome FANCONI ANEMIA. (bvsalud.org)
  • Abstract title: The Rbfox1 and Rbfox2 proteins and the control neuronal splicing programs. (csh-asia.org)
  • These findings collectively suggest that the MLK3-Pin1 signaling cascade plays a critical role in regulating the cell cycle centrosome numbers and oncogenesis. (bioskinrevive.com)
  • This study was designed to examine the influence of zinc status of HepG2 cells on the expression of Egr-1, a transcription factor that has been shown to be involved in the regulation of apoA-I gene expression. (usda.gov)
  • As a transcription factor whose expression is increased by DNA damage, p53 blocks cell division at the G1 phase of the cell cycle to allow DNA repair. (medscape.com)
  • Translocation carcinoma is a renal cell carcinoma (RCC) subtype that harbors a translocation involving a member of the microphthalmia transcription factor gene family. (medscape.com)
  • 3.0: hematopoietic cell-specific Lyn substrate 1 (HCLS1), Kelch domain-containing 6 (KLHDC6), sarcoglycan delta (SGCD), KIAA1706 protein (KIAA1706), RNA guanylyltransferase and 5′-phosphatase (RNGTT), chromosome 10 open reading frame 57 (C10orf57), chromosome 9 open reading frame 52 (C9orf52), and plasminogen activator, urokinase receptor (PLAUR). (molvis.org)
  • [ 1 ] His prediction was subsequently supported by the cloning of the retinoblastoma tumor suppressor gene ( RB1 ) and by functional studies of the retinoblastoma protein, Rb. (medscape.com)
  • Membrane-associated and cytoplasmic human chorionic gonadotropin (hCG), its subunits and its fragments were present in human cancer cells of neuroblastomas, medulloblastomas, retinoblastomas, and malignant gliomas of nude mice. (cancerprev.org)
  • Increasing evidence indicates that senescent cells could be a promising new target for therapeutic intervention known as senotherapy, which includes depleting senescent cells, modulating SASP and restoration of senescence inhibitors. (frontiersin.org)
  • Several of our studies involve the EBNA1 protein of EBV, which is expressed in both latent and lytic forms of infection, is the only viral protein needed to replicate and maintain EBV genomes in human cells and contributes to cell transformation. (utoronto.ca)
  • In addition, we have generated a library of over 200 herpesvirus proteins (from EBV, CMV and HSV-1) that are used in proteomics approaches to determine which viral proteins manipulate specific cellular processes. (utoronto.ca)
  • The X-linked tumor suppressor TSPX interacts and promotes degradation of the hepatitis B viral protein HBx via the proteasome pathway PLoS One. (usc.edu)
  • There are a number of hypothesized that regulating the transgene with a GFAP promoter in a different vectors that transduce cells in the brain in a slightly different viral vector would give rise to a high transgenic expression in the manner (for a review see, e.g. (lu.se)
  • Functional annotation clustering showed that they belong to cell cycle, organelle or nuclear lumen, protein transport, and DNA binding and repair clusters. (molvis.org)
  • The phenotype of Bard1 null mice was found to be remarkably similar to that of Brca1 nulls, further emphasising the functional relationship between these two proteins. (bmj.com)
  • We propose that the BPTI decamer, with a heparin chain threading the decamer channel, plays a functional role in the mast cell. (lu.se)
  • Bioinformatic analyses of DNA- and protein sequences. (lu.se)
  • To understand how B-Raf itself is regulated, we combined mass spectrometry with genetic approaches to map its interactome in MCF-10A cells as well as in B-Raf deficient murine embryonic fibroblasts (MEFs) and B-Raf/Raf-1 double deficient DT40 lymphoma cells complemented with wildtype or mutant B-Raf expression vectors. (uni-wuerzburg.de)
  • No mass spectrometry results found for this protein. (yeastrc.org)
  • With the help of various forms of mass spectrometry, synchrotron radiation, protein production & labelling, and bioinformatics, our facilities provide the opportunity to study protein structure and dynamics, molecular probes and drug design. (lu.se)
  • Many studies have reported their regulatory roles in immunity through cytokines and cell signaling. (cancerindex.org)
  • The proteins that play a role in stimulating cell division can be classified into four groups- growth factors , growth factor receptors , signal transducers, and nuclear regulatory proteins ( transcription factors ). (britannica.com)
  • These vectors have a number of appealing features including the expression by using the machinery of the host cell instead of depending abilities to ef®ciently transduce cells in the central nervous system, on recombinant regulatory proteins. (lu.se)
  • In this study, we investigated programmed death-ligand 1 (PDL-1) as a potential biomarker for CAF-induced chemoresistance and evaluated its role and the underlying mechanisms of chemoresistance in non-small cell lung cancer (NSCLC). (cdc.gov)
  • Silencing PDL-1 expression suppressed CAFs' ability to promote stem cell-like properties and invasiveness of lung cancer cells, favoring chemoresistance. (cdc.gov)
  • We are interested in the proteins and mechanisms involved in maintaining EBV genomes and host cell immortalization in latency, as well as cues that trigger EBV reactivation to the lytic cycle. (utoronto.ca)
  • In this review, we consider some of the theoretical aspects of protein haptenation, how mechanisms of protein haptenation can be investigated experimentally and how we can use such knowledge in the development of novel, alternative approaches for predicting skin sensitization potential in the future. (cdc.gov)
  • The basis of hapten- molecular mechanisms of the sensitization pro- protein binding work is the hypothesis that upon cess will result in novel opportunities for the skin absorption, only protein-reactive chemicals development of alternative methods for assessing (or those that can be metabolically or chemically skin sensitization hazard and relative potency of converted to protein-reactive species) are able to chemicals. (cdc.gov)
  • Effects of Lipofectamine 2000/siRNA complexes on autophagy in hepatoma cells Mol Biotechnol. (usc.edu)
  • The encoded protein is a ubiquitin-ligase capable of ubiquinating PCNP (PEST-containing nuclear protein), and together they may play a role in tumorigenesis. (nih.gov)
  • Stabilized cyclin D1 today up-regulates Cdk activity which eventually promotes cell-cycle development (Fig. 8). (bioskinrevive.com)
  • Inhibition of APE1/Ref-1 redox function significantly reduced NFĸB transcriptional activity, survivin mRNA and survivin protein levels. (oncotarget.com)
  • 11 The functionally important BARD1/BRCA1 heterodimer formation is mediated by the RING finger motifs and has also been shown to markedly increase the stability of both proteins. (bmj.com)
  • The identified proteins were subjected to subsequent bioinformatics analysis using the DAVID database. (molvis.org)
  • The proteasome pathway is an enzyme complex existing in all cells, which degrades ubiquitinated proteins that control the cell cycle and cellular processes and maintains cellular homeostasis. (medscape.com)
  • Here we describe a different method of transgene regulation by the use of the human glial ®brillary acidic protein (GFAP) promoter. (lu.se)
  • The p16INK4A protein is a cell-cycle inhibitor that acts by inhibiting activated cyclin D:CDK4/6 complexes, which play a crucial role in the control of the cell cycle by phosphorylating Rb protein. (medscape.com)
  • Alternative splicing results in multiple transcript variants some of which are non-protein coding. (nih.gov)
  • However, the mechanism by which nuclear translocation is regulated remains unclear. (nature.com)
  • Upon activation of MLK3 by known agonists ceramide and TNFα (27) or various other unidentified agonists MLK3 could phosphorylate Pin1 in the S138 site (Fig. S7) and promote its nuclear translocation. (bioskinrevive.com)
  • Translocation-associated renal cell carcinomas (RCCs) arise from the proximal tubule epithelium, similar to conventional clear cell and papillary RCCs. (medscape.com)
  • Xp11.2 translocation-associated renal cell carcinomas are generally cortical or subcapsular, well-circumscribed lesions with yellow-tan, variegated cut surfaces. (medscape.com)
  • Xp11.2 translocation-associated renal cell carcinomas (RCCs) may have well-developed papillae, mimicking papillary RCCs. (medscape.com)
  • The HuProt microarray results showed that the signals of 343 proteins were higher in the recombinant CRYAA group than in the control group. (molvis.org)
  • Identification of UHRF2 as a Negative Regulator of Epithelial-Mesenchymal Transition and Its Clinical Significance in Esophageal Squamous Cell Carcinoma. (nih.gov)
  • In 48 cases of squamous cell carcinoma of the larynx the thymidine labeling index was a more important predictor of survival (p=0.015) than prognostic DNA values and tumor stage. (cancerprev.org)
  • What pathways are this gene/protein implicaed in? (cancerindex.org)
  • Reversible proteasome inhibition disrupts pathways supporting cell growth, thus decreasing cancer cell survival. (medscape.com)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • PTH secretion is regulated by the action of serum ionized calcium on the calcium sensing receptor (CASR) in parathyroid chief cells. (medscape.com)
  • In S. cerevisiae, this process involves inhibition of the karyopherin/importin Kap121p (also known as Pse1p), which acts as the specific nuclear import receptor for several proteins, including Glc7p. (yeastgenome.org)
  • First, a growth factor must bind to its receptor on the cell membrane . (britannica.com)
  • The use of conventional and zonal centrifugation to study the life cycle of mammalian cells. (wikidata.org)
  • Within any tumor, nuclear size may vary, but uniformly, these tumors are of a high Fuhrman nuclear grade. (medscape.com)
  • Another characteristic architectural feature found in many of these tumors is a pseudoalveolar pattern in which cells are arranged in alveolae with central cellular discohesion (see the fourth image below). (medscape.com)
  • Psammoma bodies and scattered xanthoma cells have been described in some tumors. (medscape.com)
  • This theory holds that tumors arise from 1 of 2 undifferentiated stem cells: the excretory duct reserve cell or the intercalated duct reserve cell. (medscape.com)