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  • 2000
  • That barrier has on average, 2000 pores in it, each of which serve as a gatekeeper, either allowing a molecule through, or barring its entry. (phys.org)
  • Molecular
  • Molecular and functional characterization of the p62 complex, an assembly of nuclear pore complex glycoproteins. (rupress.org)
  • To unravel this striking difference, we present coarse-grained molecular dynamics simulations that reveal that FG-pores exhibit a high-density, nonuniform protein distribution, in contrast to a uniform and significantly less-dense protein distribution in the SG-mutant. (elifesciences.org)
  • biochemical
  • Computational and biochemical identification of a nuclear pore complex binding site on the nuclear transport carrier NTF2. (nih.gov)
  • Isolation involved biochemical complementation of cytosol depleted of this activity by preadsorption with recombinant p62 and the use of a novel flow cytometry-based assay for quantitation of nuclear import. (rupress.org)
  • nucleoplasmic
  • On the nucleoplasmic face of the NPC, so-called nuclear baskets or fishtraps are seen ( 7 - 9 ) that consist of eight fibrils that extend 100 nm from a ring at the nucleoplasmic face of the NPC and are connected by a narrower ring at their termini. (pnas.org)
  • cytosolic
  • Each spoke can be divided into almost identical cytosolic and nuclear half-spokes that each consist of ≈25 different nups ( 1 ). (pnas.org)
  • Previous studies have shown that immobilized recombinant p62 can bind the cytosolic nuclear import factor NTF2 and thereby deplete transport activity from cytosol. (rupress.org)
  • We have now found that immobilized recombinant p58 and p54 also can deplete nuclear transport activity from cytosol, and that p62, p58, and p54 bind directly to the cytosolic nuclear import factors p97 and NTF2. (rupress.org)
  • These data support a model in which the p62 complex binds to a multicomponent particle consisting of transport ligand and cytosolic factors to achieve accumulation of ligand near the central gated channel of the nuclear pore complex. (rupress.org)
  • Identification of NTF2, a cytosolic factor for nuclear import that interacts with nuclear pore complex protein p62. (rupress.org)
  • In this study we have purified a cytosolic factor that interacts with the nuclear pore complex glycoprotein p62. (rupress.org)
  • NTF2 interacts with at least one additional cytosolic transport activity, indicating that it could be part of a multicomponent system of cytosolic factors that assemble at the pore complex during nuclear import. (rupress.org)
  • Translocation
  • Cette réponse innée antivirale requiert la translocation nucléaire des facteurs transcriptionnelles IRF3 et NF-κB p65 pour la production des IFNs de type I. Un essai de microscopie a été développé afin d'évaluer l'effet du silençage de 60 gènes exprimant des protéines associés au complexe du pore nucléaire et au transport nucléocytoplasmique sur la translocation d'IRF3 et NF-κB p65 par un criblage ARNi lors d'une cinétique d'infection virale. (umontreal.ca)
  • The innate antiviral response requires the nuclear translocation of IRF3 and NF-κB p65 for the production of type I interferons. (umontreal.ca)
  • nups
  • We also report an updated screen for vesicle coating complexes, which share a common evolutionary origin with Nups, and can be traced back to LECA. (diva-portal.org)
  • selectivity
  • The NLP hydrogel effectively transports cargo complexes down to the single nanomolar range in the environment, and a selectivity of approximately 300 can be achieved between target molecule and protein impurity. (rsc.org)
  • transporters
  • This represents a versatile system for fusing nuclear transporters with diverse peptide tags, enabling the selective binding and transport of a wide variety of separations of high value biomolecules. (rsc.org)