Nuclear Localization SignalsCell Nucleusalpha KaryopherinsActive Transport, Cell NucleusNuclear Envelopebeta KaryopherinsMolecular Sequence DataAmino Acid SequenceKaryopherinsCytoplasmNuclear ProteinsRecombinant Fusion ProteinsProtein Sorting SignalsNuclear Export SignalsProtein TransportHeLa CellsGreen Fluorescent ProteinsCell LineProtein Structure, TertiaryCOS Cellsran GTP-Binding ProteinProtein BindingLuminescent ProteinsBase SequenceMutationLaminsSubcellular FractionsCell MembraneSequence Homology, Amino AcidTransfectionSequence DeletionMicroscopy, FluorescenceMembranesCercopithecus aethiopsDNA-Binding ProteinsSignal TransductionCell NucleolusCell CompartmentationBiological TransportTranscription FactorsAmino Acid MotifsIntracellular MembranesBinding SitesNucleoplasminsFluorescent Antibody TechniqueMicroscopy, ConfocalMembrane LipidsNuclear PoreCloning, MolecularMutagenesis, Site-DirectedThymopoietinsLamin Type BViral ProteinsCarrier ProteinsPlasmidsCells, CulturedSaccharomyces cerevisiae ProteinsRecombinant ProteinsDNA, ComplementaryNuclear Pore Complex ProteinsRNA-Binding ProteinsReceptors, Cytoplasmic and NuclearFluorescent Antibody Technique, IndirectSequence AlignmentBlotting, WesternPhosphorylationMembrane ProteinsMicroscopy, ElectronModels, BiologicalMembrane PotentialsNucleocytoplasmic Transport ProteinsSaccharomyces cerevisiaeTwo-Hybrid System TechniquesRNA, MessengerAmino Acids, BasicDNA PrimersNuclear LaminaConserved SequenceMembranes, ArtificialZinc FingersTranscription, GeneticProtein Isoforms3T3 CellsMutagenesisLamin Type Abeta-GalactosidaseImmunohistochemistryPhosphoproteinsMicroscopy, ImmunoelectronCell Membrane PermeabilityEndoplasmic ReticulumAntigens, Polyomavirus TransformingCytosolMembrane FluidityCricetinaeNIH 3T3 CellsGenes, ReporterNuclear MatrixCell FractionationImmunoblotting