Nuclear maturation culture in vitro oocyte maturation techniques. Medical search. Frequent questions

Herein, we aimed to investigate whether age-associated accumulation of advanced glycation end-products (AGEs) in oocytes impairs the recovery of embryos from cryopreservation-induced mitochondrial dysfunction/damage. (bvsalud.org)
Mouse eight-cell stage embryos developed in vitro were vitrified and warmed and incubated up to the blastocyst stage. (bvsalud.org)
EX527 increased mtDNA content in the spent culture medium of vitrified embryos derived from young mice. (bvsalud.org)
oxidative stress is the most prominent one, which will cause damage or alter the genetic material of the sperm and reduce the quality of the oocytes, and has a crucial impact on the possibility of developing embryos even after implantation. (vetmedmosul.com)
Oocytes, sperms, and embryos are shielded from oxidative stress in their natural environment by free radical scavengers found in oviductal and follicular fluids. (vetmedmosul.com)
Oocytes and embryos are not provided with this level of protection when in vitro production (9). (vetmedmosul.com)
Melatonin supplementation has been shown to promote bovine and porcine oocytes' maturation rate and increase their blastocyst of IVF embryos (16). (vetmedmosul.com)
The application of reproductive biotechnology tools such as estrus synchronization (ES), artificial insemination (AI), embryo transfer (ET) through multiple ovulation and in vivo collection of embryos, in-vitro embryo production (IVEP) and cryopreservation, somatic cell nuclear transfer (SCNT) to produce the desired breed and sex of animal, and other reproductive biotechnologies that enhances reproduction potential are important innovations that can address specific needs and purpose for animal reproduction. (ifory.id)
To prove the ability of mature oocytes to develop into embryos, 900 oocytes were processed for fertilization in vitro. (sciencegate.app)
The blastocyst stage of oocyte developed embryos was reached by 12.43±2.15% and 22.28±4.86% of the control and treatment groups, respectively. (sciencegate.app)
ABSTRACT We aimed to assess the effects of melatonin in the in vitro production of bovine embryos. (sciencegate.app)
Our experiment was further divided into two: the first was to assess the effect of different concentrations of melatonin (treatments) on the maturation rate of COCs, and the second was to assess the effects of melatonin treatments on the in vitro production of bovine embryos. (sciencegate.app)
As a result of a lower number of oocytes retrieved, there are fewer embryos to select and transfer, and subsequently these patients have lower pregnancy rates per transfer and lower cumulative pregnancy rates per started cycle compared with normal responders. (biomedcentral.com)
Retrospectively, it seems absurd, but the respected big-bearded academics disregarded the obvious fact that - unlike those of frogs and salamanders - oocytes and embryos of mammals are extremely sensitive to temperature changes and do not tolerate exposition to room temperature, not even for a short period. (vitavitro.com)
After that, we examined the result of cultured conditioned mass media (CM) of IVF embryos on splenic immune system cells and Madin-Darby bovine kidney (MDBK) cells with stably presented ISG15 promoter-reporter constructs. (bioskinrevive.com)
For in vivo test: In vitro lifestyle and embryo freezing After IVF, oocytes encircled with cumulus cells had been placed in fresh new TCM-199 moderate, as well as the embryos had been co-cultured with cumulus cells, as previously defined [21]. (bioskinrevive.com)
Recurrent implantation failure (RIF) refers to cases in which women have had three failed in vitro fertilization (IVF) attempts with good quality embryos. (biomedcentral.com)
Although there is no accepted formal definition for recurrent implantation failure, Orvieto suggests that it is after three failed in vitro fertilization-embryo transfer (IVF-ET) cycles with good quality embryos transferred [ 5 ]. (biomedcentral.com)

Injection of a MIR21 mimic increased oocyte maturation rate. (biomedcentral.com)
Results: The results indicated that FSH supplementation significantly increased oocyte maturation rate (65.21±7.26 vs. 43.25±6.23%) as indicated by extrusion of PB1 and homologous chromosome pairing and lined in the equator. (sciencegate.app)

In vitro maturation (IVM) is the technique of letting the contents of ovarian follicles and the oocytes inside mature in vitro. (wikipedia.org)
Of these primordial follicles, only around 400 mature oocytes are released and could be potentially fertilised, with the rest undergoing atresia. (wikipedia.org)
Primordial follicles containing the primary oocyte, arrested at prophase of meiosis I, develop into primary follicle containing cuboidal granulosa cells. (wikipedia.org)
If without the use of priming, oocytes are obtained when the largest follicles are around 10mm in size. (wikipedia.org)
biopsied human ovarian tissue and achieved a 10% rate of maturation from unilaminar follicles into metaphase II by a multi-step culture system: 8 days of culture in a serum-free medium 8 days of culture in a serum-free medium with activin A 4 days of culture on membranes with activin A and follicle-stimulating hormone (FSH). (wikipedia.org)
Mammalian ovaries are endowed with a huge number of small oocytes (primordial oocytes) in primordial follicles. (go.jp)
These protease-induced decreases in IGFBP-4 and -5 likely cause increased levels of bioavailable (or free) IGFs that stimulate steroidogenesis and mitogenesis in developing dominant follicles, which ultimately prepare the follicle(s) and oocyte(s) for successful ovulation and fertilization. (bioone.org)
Oocytes were collected from aspirated porcine tertiary follicles. (biomedcentral.com)
The follicular fluid (FF) provides a crucial microenvironment for the proper development of follicles and oocytes through tightly controlled metabolism and cell signaling. (biomedcentral.com)

This leads to improved maturation and quality of the oocytes. (wikipedia.org)

Priming is the process by which the oocytes are primed with follicle-stimulating hormone (FSH) or human chorionic gonadotrophin (hCG) before retrieval. (wikipedia.org)

Through somatic cell nuclear transfer, we finally obtain transgenic cattle with increased resistance to tuberculosis. (biomedcentral.com)
Galli, C. (2005), Somatic cell nuclear transfer in horses: effect of oocyte morphology, embryo reconstruction method and donor cell type. (rfgi.fr)
Somatic Cell Nuclear Transfer (SCNT-Cloning) is a promising technique in many areas and is based on genetically identical individuals. (pvb.com.br)

These then begin meiosis to form the oocyte within the primordial follicle. (wikipedia.org)
This follicle consists of the oocyte surrounded by flattened pregranulosa cells. (wikipedia.org)
The dominant follicle contains the mature oocyte. (wikipedia.org)
Cumulus oocyte complexes (COCs) of cows derived from follicle aspiration were divided into three groups. (sciencegate.app)
The aim of this study was to determine the in vitro maturation rate of Bligon goat oocytes supplemented with follicle-stimulating hormone (FSH), and their ability for further embryonic development after in vitro fertilization. (sciencegate.app)
Growth of the dominant follicle encompasses enlargement of the oocyte, replication of follicular cells, and formation and expansion of a fluid-filled follicular antrum or cavity, providing a specialized microenvironment for the development of oocytes. (biomedcentral.com)
The aim of this study was to assess the effects of brief, intense exposure to tobacco smoke on granulosa cell number, oocyte growth, and follicle size during puberty in female Swiss mice. (biomedcentral.com)

Conclusion: FSH supplementation significantly improves oocyte maturation and yields mature oocytes for future embryo development in vitro. (sciencegate.app)

One thousand five hundred cumulus-oocyte complexes were matured for 24 h in tissue culture medium 199 supplemented with 50 IU/L FSH or without FSH (control). (sciencegate.app)
The cumulus-oocyte complexes (COCs) were distributed among treatments at concentrations of 0, 10-1, 10-3 and 10-5 µMol/L melatonin. (sciencegate.app)

Poor in vitro embryo development is a major obstacle in porcine assisted reproduction. (mdpi.com)
In the current study, we utilized modified Spirulina maxima pectin nanoparticles as a supplement to improve porcine in vitro maturation medium. (mdpi.com)

In conclusion, melatonin could be a promising tool for improving sperm competence for fertilizing oocytes and embryo development in sheep. (vetmedmosul.com)
Choi Y H, Love L B, Varner D D, Hinrichs K. (2006), Blastocyste development in equine oocytes with low meiotic competence after suppression of meiosis with roscovitine prior to in vitro maturation. (rfgi.fr)
To characterize the effect of artificially increasing MIR21 on oocyte competence without inhibiting GVBD, a MIR21 mimic, scrambled microRNA negative control, or nuclease free water was micro-injected into denuded oocytes at 21 h of IVM. (biomedcentral.com)

When the inability of male transgenic rats to transmit the transgene is not associated with mosaicism, microinsemination techniques such as intracytoplasmic sperm injection (ICSI) and round spermatid injection (ROSI) can be used for passage of the transgenes. (go.jp)
This study was conducted to evaluate in vivo and in vitro development of in vitro-matured equine oocytes fertilized by intracytoplasmic sperm injection. (bioone.org)

During ovulation, the primary oocyte will resume meiosis in response to signals, arresting in metaphase meiosis II, ready for fertilization. (wikipedia.org)

These microinsemination techniques can be combined with cryopreservation of sperm and spermatid cells, leading to successful transmission of transgenes carried in male gametes. (go.jp)
Assisted reproductive techniques for canines: preservation of genetic material in domestic dogs [3] "Assisted reproductive techniques (ARTs), such as artificial insemination, in vitro fertilization, and cryopreservation of gametes/zygotes, have been developed to improve breeding and reproduction of livestock and for the treatment of human infertility. (edu.au)

In vivo development was assessed after transfer of injected oocytes to the oviducts of recipient mares. (bioone.org)
This review focuses on the differential responses of cell types to ATZ toxicity, testicular effects of ATZ in both in vitro and in vivo models and chemopreventive strategies, so as to highlight the current state of the art on the toxicological outcomes of ATZ exposure in several experimental model systems. (frontiersin.org)
Components and OPTIONS FOR in vivo test: In vitro maturation and fertilization maturation and fertilization had been carried out as described [21] previously. (bioskinrevive.com)

Oocytes were collected from slaughterhouse-derived ovaries, matured in vitro, and injected with frozen-thawed stallion sperm. (bioone.org)

This study aims to determine the ability of in vitro embryo cleavage of Bali cattle 48 hours after the fertilization process based on different ovarian reproductive status. (ifory.id)
After 48 hours the culture was evaluated for the ability of embryo cleavage. (ifory.id)
Understanding the association between Cell-free DNA levels in embryo CM and the quality of embryo cleavage could help improve the quality of IVF techniques. (who.int)

Utilizing high-throughput sequencing technology and quantitative real-time PCR (qPCR), we found that the lncRNA, loc100847420, was highly enriched in bovine sperm and was carried into the oocyte during fertilization. (bvsalud.org)
Our ICSI procedure includes the use of a piezo-driven small-sized injection pipette (2-4 μm in diameter), isolated sperm heads, and freshly ovulated oocytes. (go.jp)
For this purpose, we have incubated ram sperm using four different treatments in terms of antioxidants: melatonin, cysteamine, vitamin C, and vitamin E. They were incubated by the standard methods of maturation and capacitation of sperm. (vetmedmosul.com)
In the last decade, in vitro fertilization and sperm capacitation techniques have significantly advanced. (vetmedmosul.com)
To fertilize the oocytes, sperm must undergo a series of physiological and biochemical alterations in vitro (3). (vetmedmosul.com)
Oxidative stress is one of the key factors contributing to a decrease in sperm quality during the maturation and capacitation of the sperm (5). (vetmedmosul.com)
thereby, oocytes and sperm are highly vulnerable to reactive oxygen species (ROS) attacks, affecting their quality and viability (6). (vetmedmosul.com)
ROS are free radicals that are continuously produced by oxidative metabolic reactions and increase in vitro conditions, causing aging of both the oocyte (7) and sperm (8), as well as perhaps impairing fertilization and embryo development. (vetmedmosul.com)
Bedford S J, Kurokawa M, Hinrichs K, Fissore R A(2003), Intracellular calcium oscillations and activation in horse oocytes injected with stallion sperm extracts or spermatozoa. (rfgi.fr)

Through in vitro maturation, folliculogenesis and latter parts of oogenesis are being mimicked outside of the ovaries- trying to recreate the conditions for these processes. (wikipedia.org)
In this review, we summarize the results regarding the activation of primordial oocytes in neonatal and adult ovaries of different species and propose a model in which ovaries of neonatal mammals contain a mixed population of both quiescent and activated primordial oocytes, while almost all primordial oocytes are quiescent in adult females. (go.jp)
FOXO3 is considered one of the molecules responsible for the dormancy of primordial oocytes in adult ovaries. (go.jp)

In control group (P0), the COCs were matured in vitro in a maturation medium without urea addition, meanwhile in the P1 and P2 groups, the medium was added with urea 20 and 40 mg/dL, respectively. (sciencegate.app)

Maturation of the mammalian oocyte is a complex process involving internal checkpoints and bidirectional communication with the surrounding cumulus cells. (biomedcentral.com)
A role for the complex in mammalian synapse formation and neurite outgrowth has been suggested by the early presence of Sec6/Sec8 immunoreactivity at sites of synaptogenesis in culture (Hazuka, 1999) and the impairment of neurite outgrowth with dominant-negative forms of sec10 and sec8 in PC12 cells (Vega, 2001). (sdbonline.org)
In the fifties of the last century, we had already the nuclear bomb, the sputnik, the television, the first digital computers - but not a single mammalian embryo produced in vitro . (vitavitro.com)

A small number of primordial oocytes start to grow, while others remain quiescent. (go.jp)
These quiescent primordial oocytes are activated, perhaps by certain mechanisms involving the interaction between stimulatory and inhibitory factors, to enter the growth phase. (go.jp)
Following germinal vesicle break down (GVBD) the oocyte is transcriptionally quiescent until fertilization and activation of the embryonic genome, occurring at the four-cell stage of development in the pig [ 9 ]. (biomedcentral.com)

Poor ovarian responders (POR) are women undergoing in-vitro fertilization who respond poorly to ovarian stimulation, resulting in the retrieval of lower number of oocytes, and subsequently lower pregnancy rates. (biomedcentral.com)

The oocytes were fertilized by spermatozoa that had been capacitated with two groups of fertilization media, the first group containing melatonin and the second group containing cysteamine. (vetmedmosul.com)
Further, melatonin and its receptors play a significant role in antioxidant defense mechanism of ovary for follicular growth and maturation. (jrhm.org)
Usage of melatonin during in vitro fertilization (IVF) procedures has been found to improve oocyte quality, survival, and fecundity. (jrhm.org)
Therefore, in future, melatonin can be implicated as preferable therapeutic especially in IVF and assisted reproductive techniques. (jrhm.org)

The in vitro embryo production (IVEP) is very stressful for gametes. (vetmedmosul.com)
As a result, developing a culture medium capable of effectively reducing ROS in vitro embryo production (IVEP) protocols is of tremendous importance (10). (vetmedmosul.com)

Inhibition of nuclear meiotic maturation via IBMX significantly increased MIR21 and decreased its target, PDCD4. (biomedcentral.com)

Germinal vesicle breakdown (GVBD) occurs during oocyte meiotic maturation, a period when transcriptional processes are virtually inactive. (biomedcentral.com)
Our results indicate MIR21 is active and important during meiotic maturation of the oocyte. (biomedcentral.com)
The oocyte must maintain arrest at the diplotene stage until meiotic resumption occurs. (biomedcentral.com)
Of particular interest is understanding the role of non-coding RNA in regulating meiotic checkpoints in the oocyte, particularly that of microRNA-21 (MIR21). (biomedcentral.com)

They include metabolic products highly associated with ovarian function - amino acids for regulating pH and osmolarity, lipids such fatty acids and cholesterols for oocyte maturation, and glucocorticoids for ovarian steroidogenesis. (biomedcentral.com)

Aceto-orcein staining was conducted to evaluate the maturation of oocytes based on the achievement of metaphase II phase that is indicated by the presence of metaphase plate and/or first polar body. (sciencegate.app)

First, matured oocytes were evaluated its morphology based on the expansion of cumulus cells and PB1 extrusion. (sciencegate.app)

Li X, Morris LHA & Allen WR (2002) In vitro development of horse oocytes reconstructed with the nuclei of fetal and adult cells. (rfgi.fr)

nuclear maturation of equine oocytes. (rfgi.fr)
In vitro nuclear maturation of compact and expanded equine oocytes treated with Roscovitine. (rfgi.fr)

It can be offered to women with infertility problems, combined with In Vitro Fertilization (IVF), offering women pregnancy without ovarian stimulation. (wikipedia.org)
In patients designated as "poor responders," so-called due to poor response to ovarian stimulation given during IVF workup, the limited number of obtained oocytes remains the major problem in optimizing the live birth rates [ 3 ]. (biomedcentral.com)

In 1935, Pincus & Enzmann did the first experiment on immature rabbit oocyte, showing in vitro spontaneous maturation and fertilization. (wikipedia.org)
Moreover, the in vitro experiment demonstrated high glucose amounts' deleterious effect on embryo development [ 3 ]. (ejast.org)

The oocytes are then cultured in media containing nutrients important for oocyte survival, such as gonadotrophins, growth factors and steroids. (wikipedia.org)
When in vitro experimental models are exposed to ATZ, they are faced with increased levels of reactive oxygen species (ROS), cytotoxicity and decreased growth rate at dosages that may vary with cell types. (frontiersin.org)

After maturation, in vitro fertilization was carried out using TALP media with a final concentration of spermatozoa 1.5 x 106 spermatozoa/ml. (ifory.id)

Oocytes were considered mature when they reached metaphase II. (sciencegate.app)

Moreover, brief exposure during puberty may induce silent oocyte disruption, which could in turn lead to decreased fecundity rates. (biomedcentral.com)

These findings are linked to melatonin's ability to reduce ROS and its adverse effects on oocytes, such as aging, apoptosis, and its capacity to bind to specific DNA sequences and interact with cytosolic molecules to regulate antioxidant genes (17). (vetmedmosul.com)

Next, to find out regardless of whether pre or post synaptic professional tein synthesis is necessary for NT 3 mediated long term synaptic modulation, we expressed GyrB PKR in either spinal neurons or myocytes applying precisely the same embryo injection techniques described over. (narturalproduct.com)
Cultures have been incubated with NT 3 for two days with or without coumermycin as indicated, At naive synapses, coumermycin treatment method didn't impact basal synaptic transmission nor prevent the long term poten tiating result of NT 3, Expres sion of GyrB PKR in either presynaptic spinal neurons or postsynaptic muscle cells devoid of coumermycin treat ment didn't alter the long lasting impact of NT three. (narturalproduct.com)

They showed maturation occurs in isolation from normal follicular environment. (wikipedia.org)

Sister branches, including invertebrate or reptilian developmental biology, were much more successful - by making nuclear transfer cloned offspring far before the First World War (no misprint - even in the 19th century). (vitavitro.com)

Next, 600 oocytes were then stained with 1% aceto-orcein to examine maturation based on changes in the configuration of chromosomes and nuclear membrane breakdown. (sciencegate.app)

72 hours) to C8- ceramide (5 μg/ml culture medium), a cell-permeable analog of ceramide, enhanced MDR1 mRNA levels by 3- and 5-fold in T47D and inMDA-MB-435 cells, respectively, but did not affect MCF-7 cells.D-erythro-sphingosine exposure (5 μg/ml, 72 hr) increased MDR1 mRNA levels in MDA-MB-435 cells by 3.5-fold. (sphingolipidclub.com)
Acute exposure ofMCF-7 and MDA-MB-231 cells to C8-glucosylceramide (10 μg/ml culture medium), a cell-permeable analog of glucosylceramide, increased MDR1 mRNA levels by 2- and 4-fold, respectively. (sphingolipidclub.com)

On observe alors que ces conceptions biologiques complexes actuelles sont de plus en plus soumises à des impératifs économiques, notamment la fiabilisation des cycles de conception et de mise sur le marché. (rfgi.fr)

The selected oocytes are then matured in an incubator of 5% CO2, a temperature of 38.5 °C for 24 hours. (ifory.id)
Oocytes 5-6 after fertilization were washed 3 times using CR1aa media then cultured in 5% CO2 incubator, temperature 38.5 °C for 48 hours. (ifory.id)

This study was aimed to determine the effect of urea in maturation medium on in vitro oocyte maturation rate. (sciencegate.app)

Matured oocytes had been inseminated with frozen-thawed semen from order MK-0822 a Japanese Dark bull (altered to 2 107 cells/ml) for 5 h at 38.5C within a humidified atmosphere with 5% CO2 in the surroundings, in 1 ml of BO solution containing 10 mg/ml bovine serum albumin and 10 g/ml heparin. (bioskinrevive.com)

It could be concluded that the presence of urea at 40 mg/dL in maturation medium reduced the percentage of bovine oocyte maturation in vitro. (sciencegate.app)

We think that primordial oocytes in adult mammals are different from those in neonatal mammals. (go.jp)
The dormancy of primordial oocytes may be required to reserve the non-growing oocyte pool for the long reproductive life in mammals. (go.jp)

This study aimed to determine the influence of antioxidants on the achievement of In vitro culture (IVC) and sperm's ability to adhere to and penetrate further into In vitro maturated oocytes. (vetmedmosul.com)

Thus, the maturing oocyte is reliant on processes such as post-transcriptional gene regulation (PTGR) to regulate the mRNA and protein repertoire. (biomedcentral.com)

Anatomy7

Chemicals and Drugs1

Analytical, Diagnostic and Therapeutic Techniques and Equipment4

Phenomena and Processes6

Embryos18

Increased oocyte maturation rate2

Follicles9

Quality of the oocytes1

Primed with follicle-stimula1

Somatic cell nuc3

Follicle7

Mature oocytes1

Cumulus-oocyte complexes2

Porcine2

Competence3

Intracytoplasmic sperm inj2

Ovulation1

Cryopreservation2

Vivo3

Injected with frozen-thawed1

Cleavage3

Sperm9

Ovaries3

COCs1

Mammalian3

Quiescent3

Undergoing in-vitro fertili1

Melatonin4

IVEP2

Significantly increased1

Meiotic4

Steroidogenesis1

Conducted to evaluate1

Morphology1

Fetal1

Equine2

Ovarian stimulation2

Experiment2

Growth2

Spermatozoa1

Metaphase1

Fecundity1

Apoptosis1

Neurons2

Occurs1

Developmental biology1

Breakdown1

Exposure2

Cycles1

Incubator2

Rate1

38.51

Percentage1

Mammals2

Achievement1

Processes1