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  • transport
  • This behavior gives a basis for meeting the intrinsically conflicting high affinity and high flux requirements of an efficient nuclear transport system. (osti.gov)
  • highly conserved among
  • This C-terminal AF2 core region, which is relatively highly conserved among nuclear receptors, undergoes an allosteric change upon ligand binding, and deletion or point mutations in this region often impair transcriptional activation without changing ligand and DNA binding affinities ( 36 ). (asm.org)
  • cascades
  • The activating signals are transduced by signaling cascades that converge on the IκB kinases (IKKs) that phosphorylate the NF-κB inhibitory molecules, including members of the IκB family and inhibitory domains of NF-κB precursors, leading to their ubiquitination and subsequent degradation by the proteasome. (biologists.org)
  • regulators
  • In addition, we used soft agar assay, immunoblotting, flow cytometry, and immunofluorescence confocal microscopy for examinations of nMET functions including stem-like cell formation, cell signaling, cell cycle regulation, and co-localization with regulators of cell signaling. (springer.com)
  • nucleocytoplasmic
  • Employing a number of mutants of Hdm2 that are defective in either nuclear import or nuclear export, we demonstrate in this report that the nucleocytoplasmic shuttling of Hdm2 is essential for its ability to promote p53 degradation. (pnas.org)
  • sequences
  • Typically, this signal consists of one or more short sequences of positively charged lysines or arginines exposed on the protein surface. (wn.com)
  • These sequences are believed to interact with specific receptors at the Nuclear pore . (jove.com)
  • transcriptional
  • Transcriptional activation of nuclear receptors appears to involve at least two separate processes: derepression and activation. (asm.org)
  • genes
  • In Xenopus embryos, depletion of IQGAP1 reduced Wnt-induced nuclear accumulation of DVL, and expression of Wnt target genes during early embryogenesis. (paperity.org)
  • specific nuclear
  • This feature relies on the presence of a specific nuclear localization signal (NLS) within the viral matrix protein (MA p17), which to some degree can be complemented by the activity of the viral vpr gene product. (biomedcentral.com)
  • Deletion of the N-terminal 34 amino acids or selective mutation of the nonapeptide (25)RRTALRPRR(33), located in the N-terminal region of HSV-1 TK, resulted in the loss of the specific nuclear localization of HSV-1 TK. (ox.ac.uk)
  • We found that the sites containing the amino acids R236-R237 and K317-R318 are absolutely required for specific nuclear targeting of HSV-1 TK. (ox.ac.uk)
  • ligand
  • These coactivators are postulated to function in transmitting the signal of ligand-induced conformational change to the basal transcription machinery. (asm.org)
  • receptors
  • The ASC-1 binding sites involve the hinge domain but not the C-terminal AF2 core domain of nuclear receptors. (asm.org)
  • Functional analysis of nuclear receptors has shown that there are two major activation domains. (asm.org)
  • As expected, many coactivators fail to interact with nuclear receptors mutated for the C-terminal AF2 core region ( 6 , 28 , 50 ). (asm.org)
  • motif
  • The green fluorescent protein (EGFP)-NES2 fusion protein was localized in the cytoplasm and its nucleo-cytoplasmic shuttling was blocked by the CRM1 specific export inhibitor leptomycin B. Mutation of a leucine residue to alanine (L135A) in the NES2 motif resulted in both cytoplasmic and nuclear localization of the EGFP-NES2 fusion protein and in the nuclear accumulation of ectopic full-length myc-Ngn3. (sigmaaldrich.com)
  • export
  • Cyclin B1 is essential for mitosis in mouse embryos, and its nuclear export sets the time for mitosis. (icr.ac.uk)
  • cancers
  • Thus, nEGFR has an old origin resembling the cancer evolution, but TM may interfere NLS driven signaling for natural selection of survival to evade NLS induced aggressive cancers. (jcancer.org)
  • For example, dysregulated nuclear transport of NF- κB is associated with cancers ( 2 , 7 , 8 ). (jcancer.org)
  • resistance
  • Taken together, our data suggest a link between nuclear RTK and cancer cell evolutionary clearance via cell death, and drug resistance for survival through stemness selection. (springer.com)
  • Mice
  • In hairless mice, as well as humans with congenital atrichia, the absence of hr gene function initiates a premature and abnormal catagen due to a dysregulation of apoptosis and cell adhesion, and defects in the signaling required for hair follicle remodeling. (biologists.org)