• RGPR-p117, which was named as a regucalcin gene promoter region-related protein, was originally discovered as a novel transcription factor that specifically binds to a nuclear factor I (NFI) consensus motif TTGGC(N)6CC that is located on the 5'-flanking region of the regucalcin gene (rgn) in 2001. (wikipedia.org)
  • Overexpression of RGPR-p117 did not cause an alteration of cell proliferation and led to significant decreases in protein and DNA contents in cloned normal rat kidney proximal tubular epithelial NRK52E cells. (wikipedia.org)
  • Description Western Blot analysis of Rat Brain Membrane showing detection of ~140 kDa Brevican protein using Mouse Anti-Brevican Monoclonal Antibody, Clone S294A-6 (56541). (qedbio.com)
  • Deep RNA-DNA, RNA-protein interaction studies, and phenotype rescue analyses reveal that LETR1 is a nuclear trans-acting lncRNA modulating, via key epigenetic factors, the expression of essential target genes, including KLF4 and SEMA3C , governing the growth and migratory ability of LECs. (nature.com)
  • 2. Standard research tool to analyze a variety of molecular biology procedures, for example, cell senescence, cytokine induction, protein expression, apoptosis, and receptor-ligand interactions. (ubigene.us)
  • A Nph3-KI targeting vector was designed in a way to result in the generation of a bicistron ic mRNA, encoding for a fusion Nph3-Flag protein, as well as for beta-galactosidase fused to a nuclear localization signal. (uni-goettingen.de)
  • 1998). In parallel to the in vivo approach, a bacterial protein expression system was established, leading to the production of recombinant Nph1 and Nph3 for use in future in vitro experiments.Analysis of Nph3 expression in Nph3-KI mice was performed by assaying brain sections for beta-galactosidase activity, based on the co-expression of beta-galactosidase from Nph3-expressing cells. (uni-goettingen.de)
  • This method revealed the Nph3 expression pattern with high accuracy and sensitivity, suggesting a novel approach for the detailed in vivo identification of cells that express a particular protein. (uni-goettingen.de)
  • T-cell leukemia/Lymphoma Protein 1A (TCL-1A), also known as p14TCL1 is a product of the TCL1 gene that is involved in T-cell prolymphocytic leukemia (TPLL). (enquirebio.com)
  • The BRCA2 R3052W mutated protein exacerbates genome instability, is unable to rescue homology-directed repair, and fails to complement cell survival following exposure to PARP inhibitors and crosslinking drugs. (frontiersin.org)
  • In vivo cloning and characterization of a new growth suppressor protein TOE1 as a direct target gene of Egr1. (nih.gov)
  • In cell fractionation research, the worth under each street in the Traditional western mark images shows the comparable sums of protein comparable to the control group. (techblessing.com)
  • The value was acquired by the intensity percentage between the target protein and histone H3 band for nuclear fractionation in each lane. (techblessing.com)
  • PR domain-containing protein 9 (PRDM9) is a major regulator of the localization of meiotic recombination hotspots in the human and mouse genomes. (springer.com)
  • To examine the involvement of interchromatin granule clusters (IGCs) in transcription and pre-mRNA splicing in mammalian cell nuclei, the serine-arginine (SR) protein kinase cdc2-like kinase (Clk)/STY was used as a tool to manipulate IGC integrity in vivo. (rupress.org)
  • The first described CID systems involve microbe-derived dimerizing ligands, such as rapamycin, that direct protein neo-associations that rewire eukaryotic cell signaling 5 . (nature.com)
  • NFKB1 (p50) is a 50KDa functional sub-unit of NF-kB and a member of Rel protein family which is synthesized as a p105 precursor protein and consists of an N-terminal conserved RHD-region containing nuclear localization signal, DNA-binding and dimerization domains. (thermofisher.com)
  • These attachment sites are linked to actin-bundles that surround the nucleus via an Ndj1-Mps3-Csm4 protein bridge that spans the inner and outer nuclear membranes. (biorxiv.org)
  • NAD-dependent protein deacetylase sirtuin-1 (SIRT1) links transcriptional regulation directly to intracellular energetics and participates in the coordination of several separated cellular functions such as cell cycle, repsonse to DNA damage, metabolism, apoptosis, and autophagy. (thermofisher.com)
  • It is essential in skeletal muscle cell differentiation and in response to low nutrients mediates the inhibitory effect on skeletal myoblast differentiation which also involves 5'-AMP-activated protein kinase (AMPK) and nicotinamide phosphoribosyltransferase (NAMPT). (thermofisher.com)
  • Cytoskeletal proteins such as dynamin (see IPR001401 ), Caenorhabditis elegans kinesin-like protein unc-104 (see IPR001752 ), spectrin beta-chain, syntrophin (2 PH domains) and S. cerevisiae nuclear migration protein NUM1. (embl.de)
  • We have previously demonstrated the utility and developmental neutrality of enhanced green fluorescent protein (EGFP) in embryonic stem (ES) cells and mice [ 2 ]. (biomedcentral.com)
  • In this study we have used embryonic stem (ES) cell-mediated transgenesis to test the enhanced cyan fluorescent protein (ECFP) and enhanced yellow fluorescent protein (EYFP), two mutant and spectrally distinct color variants of wild type (wt) GFP. (biomedcentral.com)
  • We have also tested DsRed1, the novel red fluorescent protein reporter recently cloned from the Discostoma coral by virtue of its homology to GFP. (biomedcentral.com)
  • This encoded protein can induce G1 arrest in the cell cycle. (appliedbioinfo.com)
  • The breast and ovarian cancer susceptibility gene BRCAl has been recently cloned and revealed an open reading frame of 1863 amino acids, but a lack of significant homology to any known protein in the database has led to few clues about its functions. (um.es)
  • A beta-hairpin comprising the nuclear localization sequence sustains the self-associated states of nucleosome assembly protein 1. (colorado.edu)
  • The predicted amino acid sequence contains a high concentration of charged amino acids in the carboxy terminal quarter of the molecule, three guanosine 5′-triphosphate (GTP)-binding protein motifs, and a consensus nuclear localization signal. (elsevierpure.com)
  • Immunohistochemical analysis of tissue sections with affinity-purified antiserum raised against a recombinant Ngp-1 protein revealed that the antigen was exclusively localized to the nucleolus and nucleolar organizer regions in all cell types analyzed. (elsevierpure.com)
  • Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Brevican Monoclonal Antibody, Clone S294A-6 (56541). (qedbio.com)
  • Both immunofluorescence and transmission electron microscopy analyses of cells overexpressing Clk/STY indicate that IGC components are completely redistributed to a diffuse nuclear localization, leaving no residual structure. (rupress.org)
  • Immunofluorescence analysis was performed on MCF7 cells (untransfected, panel a-d), transfected with SIRT1 specific siRNA (panel i-l) or non-specific scrambled siRNA (panels e-h). (thermofisher.com)
  • Epstein, A.L. and Clevenger, C.V., Identification of nuclear antigens in human cells by immunofluorescence, immunoelectron microscopy, and immuno-biochemical methods using monoclonal antibodies. (neobiotechnologies.com)
  • 2012 ), generalizing the observation that hotspot localization is determined by the DNA-binding specificity of the zinc finger array (Grey et al. (springer.com)
  • Furthermore, two mAb hybridoma clones, 2A9-B12 and 8G11-B2 derived from 2A9 and 8G11, respectively, displayed high specificity for Meq proteins of MDV-1 strains with diverse virulence. (pirbright.ac.uk)
  • The efficacy and specificity of CRAD on prostate cancer cells were examined in cell culture and animal models. (hindawi.com)
  • Specificity: The new marker p40 (DeltaNp63) is highly specific for squamous basal cells. (scytek.com)
  • mTOR-dependent cytoplasmic retention of TFE3 promotes the exit of hematopoietic stem cell from pluripotency (PubMed:30733432). (neobiotechnologies.com)
  • RGPR-p117 is present in the plasma membranes, cytoplasm, mitochondria, microsomes and nucleus of the cells. (wikipedia.org)
  • Localization: Cell Membrane, Nucleus. (qedbio.com)
  • AEG-1 has been shown to localize to the cell membrane, cytoplasm, endoplasmic reticulum, nucleolus and nucleus in cancer cells ( 2 ). (spandidos-publications.com)
  • The NFkB complex is expressed in most cell types, and is primarily found in the cytoplasm in an inactive state in association with I-kappa-B. Phosphorylation of I-kappa-B releases the complex, allowing translocation to the nucleus and modification of gene expression. (thermofisher.com)
  • Prior to the first meiotic division, homologous chromosomes pair, synapse, and recombine to ensure their proper disjunction at anaphase I. Additionally, telomeres tethered at the nuclear envelope cluster in the bouquet configuration where they are subjected to dramatic pulling forces acting from outside of the nucleus. (biorxiv.org)
  • BRCA2 nuclear localization and export sequences are listed. (frontiersin.org)
  • The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. (thermofisher.com)
  • However, it is difficult to capture the complexity of transcript isoform variance using these libraries, and large cDNA sequences are often difficult to clone into size-limited viral expression vectors. (cdc.gov)
  • Phosphorylated RGPR-p117 specifically binds to the TTGGC motif in the promoter region of various genes to enhance the gene expression of various proteins, and plays a crucial role as a transcription factor in the cells. (wikipedia.org)
  • Conversely, overexpression of a catalytically inactive mutant, Clk/STY(K190R), causes retention of hypophosphorylated SR proteins in nuclear speckles. (rupress.org)
  • Here we identified Nup2 in a pool of enriched proteins that co-purify with tagged Ndj1 from meiotic cell extracts. (biorxiv.org)
  • Their N-terminal hydrophilic domains may interact with cytoskeletal proteins and therefore play a cell structural role. (tcdb.org)
  • All AE proteins are hypothesized to share a similar topology in the cell membrane. (tcdb.org)
  • Several S. cerevisiae proteins involved in cell cycle regulation and bud formation like BEM2, BEM3, BUD4 and the BEM1-binding proteins BOI2 (BEB1) and BOI1 (BOB1). (embl.de)
  • The adenovirus early region 1 (E1) gene, which comprises E1a and E1b, encodes the viral early proteins that are necessary for adenoviral replication and the consequent oncolysis of permissive host cells. (hindawi.com)
  • The primary barriers for invading respiratory pathogens are the respiratory tract epithelial cells and antimicrobial proteins generated by these cells. (cdc.gov)
  • This method of construction not only makes up for the time-consuming and laborious defect of traditional methods used to construct infectious cDNA clones, but also avoids the toxicity of the Potyvirus special sequence to Escherichia coli , thus providing a useful cloning strategy for the construction of infectious cDNA clones for other viruses and laying down a foundation for the further investigation of SMV cross-family infection mechanisms. (ppjonline.org)
  • Potential features that attract the investigators are epithelial cells with squamous nature and responsible for the diffusion of substances, such as water and electrolytes, across the alveoli of the lungs. (ubigene.us)
  • A549 cells used as a type II pulmonary epithelial cell model for drug metabolism and as a transfection host. (ubigene.us)
  • Significant downregulation of beta-defensin1 gene (DEFB1) expression was observed when human bronchial epithelial cells (HBEpCs) were exposed to IAV. (cdc.gov)
  • This gene expression is stimulated through various signaling factors in cells. (wikipedia.org)
  • Furthermore, the expression of the CYCA gene (involved in cell cycle and proliferation) was significantly downregulated in NAV and TDL isolates. (unav.edu)
  • During development, the blood vascular system arises from endothelial cell progenitors that differentiate from mesodermal cells, mostly through the expression of the transcription factor (TF) ETV2. (nature.com)
  • Activation of the VEGFA/VEGFR2 signaling and expression of blood vascular endothelial cell (BEC) markers, such as NRP1 and EphrinB2, further differentiate these precursor cells into BECs, which then form the hierarchical network of blood vessels 4 . (nature.com)
  • Key molecular studies performed with A549 cell as anti-tumor drug permeability and efficacy analysis, infection assays, respiratory immunotoxicity tests, cell senescence studies, and cytokine expression profiling. (ubigene.us)
  • A highly localized Nph3 expression pattern was observed with the most characteristic area of expression being the deep layers of the neocortex, where a band of expressing cells persisted throughout ontogeny and adulthood. (uni-goettingen.de)
  • In addition, expression of Nph3 was observed in a number of transient cell populations in the marginal zone of the developing neocortex, the dentate gyrus of the hippocampal formation and the granule cell layer of lobules 9 and 10 of the cerebellum, where low expression levels were also observed in adulthood. (uni-goettingen.de)
  • Ubiquitous mainly nuclear expression. (neobiotechnologies.com)
  • In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity (By similarity). (neobiotechnologies.com)
  • Molecular cloning of the cDNA for the catalytic subunit of plant DNA polymerase alpha and its cell-cycle dependent expression. (neb.com)
  • Flow cytometric analysis of Akt (pS473) expression in human Jurkat cells. (bdbiosciences.com)
  • We demonstrate that WG and DPP act together through their targets Distal-less ( Dll ) and dachshund ( dac ) to restrict hth expression, and therefore EXD's nuclear localization, to the most proximal regions of the leg disc. (biologists.com)
  • Furthermore, there is a reciprocal repression exerted by HTH on these and other DPP and WG downstream targets that restricts their expression to non- hth -expressing cells. (biologists.com)
  • To this end, we have established lines of ES cells together with viable and fertile mice having widespread expression of either the ECFP or EYFP GFP-variant reporters. (biomedcentral.com)
  • A cDNA clone encoding an immunoreactive autoantigen (Ngp-1) was isolated by screening λgt11 human ductal breast tumor expression libraries with autologous patient serum. (elsevierpure.com)
  • Similarly, GATA-1 has been shown to induce lineage switching expression values even if, for simplicity, we assume only ``on'' of committed cells in hematopoiesis, first in cell lines (Kulessa and ``off'' states for each gene. (lu.se)
  • In reality, gene somatic cells to a pluripotent cell state by a handful of transcrip- expression is graded, making the potential gene expression tion factors (Takahashi and Yamanaka, 2006). (lu.se)
  • A . Schematic of the bouquet configuration of chromosomes during meiotic prophase I. The spatial arrangement of chromosomes with telomeres clustered and attached at the inner nuclear membrane is shown. (biorxiv.org)
  • AE1, AE2, and NBCn1), (ii) is important for trafficking to the cell membrane (e.g. (tcdb.org)
  • 16. A living cell has a protoplasm which is water based and demarcated by a lipid bilayer membrane. (pharmatutor.org)
  • Immunofluorescent staining of PFA-fixed human HeLa cells with Cyclin B1 antibody (clone V92.1). (nsjbio.com)
  • SDS-PAGE analysis of purified, BSA-free Cyclin B1 antibody (clone V92.1) as confirmation of integrity and purity. (nsjbio.com)
  • TOE1 is an inhibitor of HIV-1 replication with cell-penetrating capability. (nih.gov)
  • Replication-competent viruses, also known as oncolytic viruses, replicate within transduced cells and force these cells into a lytic cycle. (hindawi.com)
  • In addition, Ingenuity Pathway Analysis (IPA) integrated with PCR array data showed that the JAK1/STAT3 pathway was significantly altered in cells overexpressing DEFB1, suggesting this to be one of the pathways by which defensin regulates IAV replication in HBEpCs. (cdc.gov)
  • 1990. Cell replication and unscheduled DNA synthesis (UDS) activity of low molecular weight chlorinated paraffins in the rat liver in vivo. (cdc.gov)
  • However, both the accumulation of splicing factors at sites of pre-mRNA synthesis as well as pre-mRNA splicing are dramatically reduced, demonstrating that IGC disassembly perturbs coordination between transcription and pre-mRNA splicing in mammalian cell nuclei. (rupress.org)
  • One of the first steps to investigate the function of BRCAl was to define its subcellular localization. (um.es)
  • As is apparent, the subcellular localization has been the most controversial aspect of BRCAl biology and is a key point to uncover its functions. (um.es)
  • In this paper we review the published data on subcellular localization of BRCAl with special emphasis on the antibodies and techniques used. (um.es)
  • It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. (neobiotechnologies.com)
  • It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. (neobiotechnologies.com)
  • Molecular Biology of the Cell. (wikipedia.org)
  • These cells also utilized to study a variety of molecular characteristics for human tumors in culture. (ubigene.us)
  • In order to clarify the molecular mechanisms underlying the cross-family infection of SMV, we used double-stranded RNA extraction, rapid amplification of cDNA ends polymerase chain reaction and Gibson assembly techniques to carry out SMV full-length genome amplification from susceptible soybeans and constructed an infectious cDNA clone for SMV. (ppjonline.org)
  • Infectious clones for plant viruses refer to DNA clones that have been constructed artificially using molecular biology techniques and have the same infectious activity as plant viruses under natural conditions. (ppjonline.org)
  • Address correspondence to C. Borner, Institute of Molecular Medicine and Cell Research, Center for Biochemistry and Molecular Cell Research, Albert-Ludwigs-University Freiburg, Stefan-Meier Strasse 17, D-79104 Freiburg, Germany. (rupress.org)
  • Correspondence with low molecular weight chemicals that results in the priming of chemical-specific, Prof. Dr. Stefan F. Martin, Allergy Research skin-homing CD8+ Tc1/Tc17 and CD4+ Th1/Th17 cells. (cdc.gov)
  • In vitro, both peptides were highly active against intracellular Leishmania major in mouse macrophages without exerting toxicity in host cells. (unav.edu)
  • NFkB is a nuclear transcription factor activated by various extra and intracellular stimuli such as cytokines, UV radiation, stress, in injury, and by bacterial and viral products. (thermofisher.com)
  • Plasmalemmal Cl - -HCO3 - exchangers regulate intracellular pH and [Cl - ] and cell volume. (tcdb.org)
  • Cloning and characterization of the 5'-upstream sequence governing the cell cycle-dependent transcription of mouse DNA polymerase alpha 68 kDa subunit gene. (neb.com)
  • Characterization of DNA binding, transcriptional activation, and regulated nuclear association of recombinant human NFATp. (colorado.edu)
  • Long-term Culture and Cloning of Primary Human Bronchial Basal Cells that Maintain Multipotent Differentiation Capacity and CFTR Channel Function. (academictree.org)
  • Within this cyst, germ cells synchronously go through the mitotic amplification phase, growth phase, meiosis and through differentiation until individualization. (biologists.com)
  • NFkB is involved in regulation of various cellular events including cell growth, differentiation, proliferation, apoptosis and inflammation. (thermofisher.com)
  • Cell Growth and Differentiation , 7 (2), 271-280. (elsevierpure.com)
  • Stem and progenitor cell populations are often heterogeneous, which may reflect stem cell subsets that express subtly different properties, including different propensities for lineage selection upon differentiation, yet remain able to interconvert. (lu.se)
  • A key challenge is to understand how state, but must also afford flexibility in cell-fate choice to permit the different cell-fate options confronting stem and progenitor cell-type diversification and differentiation in response to cells are selected and coordinated such that adoption of a given intrinsic cues or extrinsic signals. (lu.se)
  • Evidence the fate of stem cells has broad ramifications for biomedical suggests that during development or differentiation, cells make science from elucidating the causes of cancer to the use of very precise transitions between apparently stable ``network stem cells in regenerative medicine. (lu.se)
  • The second-largest subunit of the mouse DNA polymerase alpha-primase complex facilitates both production and nuclear translocation of the catalytic subunit of DNA polymerase alpha. (neb.com)
  • Further, NFKB1 (p50) directs the nuclear translocation of NF-kB and is instrumental in its DNA-binding. (thermofisher.com)
  • B-cell translocation gene 4, cullin 1, MCF.2 transforming sequence, a locus similar to snail soma ferritin, and three unidentified genes were, indeed, preferentially expressed in the oocyte, even though most were also highly expressed in testis. (appliedbioinfo.com)
  • B cell translocation gene 4 (BTG4) is a key adaptor of the CCR4-NOT deadenylase complex, which is involved in maternal mRNA decay in mice, but no human diseases caused by mutations in BTG4 have previously been reported. (appliedbioinfo.com)
  • Ubigene exclusive KO Cell Line Bank, over 5000 KO cell lines, covering thousands of genes from 8 popular signaling pathways and nearly 100 diseases. (ubigene.us)
  • To assess the role of an individual HDAC isoenzyme in physiology and tumor development, HDAC2-mutant mice were generated from a gene trap embryonic stem cell clone. (aacrjournals.org)
  • We discuss these properties with examples both from the hematopoietic and embryonic stem cell (ESC) systems. (lu.se)
  • A549 is a model lung cancer cell line developed in 1972 by scientist Giard, through the removal and culturing of cancerous lung tissue in the explanted tumor of a 58-year-old Caucasian male. (ubigene.us)
  • A549 cells are used in a variety of basic cell biology and biomedical studies involving lung cancer proliferation, corresponding inhibitors studies, tumor formation, and metastases. (ubigene.us)
  • However, A549 cells harbor oncogenic KRASG12S mutation as well as over 250 genetic mutations (COSMIC cell lines project), including some known oncogenes and tumor suppressors. (ubigene.us)
  • Outcomes c-Fos Is normally Involved in the Nuclear Transfer of HBXIP In our prior research, we reported that HBXIP is expressed in medical breasts tumor cells highly. (techblessing.com)
  • Many tumor cell lines and experimental tumors respond to HDAC inhibition. (aacrjournals.org)
  • Identification of the nuclear localization signal of mouse DNA primase: nuclear transport of p46 subunit is facilitated by interaction with p54 subunit. (neb.com)
  • To systematically discover ovarian fertility genes en masse, we employed a mouse model (Foxo3) in which follicles are assembled normally but then undergo synchronous activation. (appliedbioinfo.com)
  • Comparative profiling of other tissues, including microdissected oocytes and somatic cells, revealed distinct gene classes and provided new insights into oogenesis and ovarian function, demonstrating the utility of our approach for tissue-specific gene discovery. (appliedbioinfo.com)
  • Adhesion formation inhibits contact-induced cellular overlap leading to nematic organization of cells and an alignment of focal adhesions. (bvsalud.org)
  • (B) Western blot of total cellular lysates from DLD-1 BRCA2 −/− cells stably transfected with full-length BRCA2 cDNA constructs: BRCA2 Wild Type (WT) and BRCA2 R3052W (1 and 2 correspond to two independent clones). (frontiersin.org)
  • (F) Western blot of total cellular lysates from DLD-1 parental cells (these cells express a wild-type allele of BRCA2) stably transfected with R3052W (3 and 5 correspond to two independent clones) full-length 2XMBP-BRCA2 cDNA constructs. (frontiersin.org)
  • Meiosis is a specialized cellular program required to create haploid gametes from diploid parent cells. (biorxiv.org)
  • In terminally differentiated cell fate is coupled to appropriate regulation of the alternative cells, transcriptional networks must be stable and irreversible, pathways. (lu.se)
  • TCL1 is overexpressed in Burkitt lymphoma, the majority of AIDS-related non-Hodgkin lymphoma-designated immunoblastic plasmacytoid lymphoma, lymphoblastic lymphoma, chronic lymphocytic leukemia, mantle cell lymphoma, follicular lymphoma, diffuse large B-cell lymphoma, and primary cutaneous B-cell lymphoma. (enquirebio.com)
  • Tonsil or T-cell Lymphoma. (enquirebio.com)
  • Cells were fixed, permeabilized, and labeled with Anti-SIRT1 Recombinant Rabbit Monoclonal Antibody (Product # 703653, 1:100 dilution), followed by Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Produ. (thermofisher.com)
  • To varying degrees, these fates also extend to the Such state stability is required in stem and progenitor cells to immediate progeny of stem cells, known as progenitor or support self-renewal and maintenance of the uncommitted transit-amplifying cells. (lu.se)
  • Keloid fibroblasts produce elevated levels of IL-6, and autocrine IL-6 production is both necessary and sufficient to induce cell and ECM alignment, as evidenced by ligand stimulation of normal dermal fibroblasts and treatment of keloid fibroblasts with the function blocking IL-6 receptor monoclonal antibody, tocilizumab. (bvsalud.org)
  • Formalin-fixed, paraffin-embeddedhuman tonsil stained with Pan-Nuclear AntigenRecombinant Mouse Monoclonal Antibody (rNM106). (neobiotechnologies.com)
  • In the role in the regulation of cell regulation, RGPR-p117 possesses protective effects on apoptotic cell death induced by various signaling factors. (wikipedia.org)
  • We introduced the human missense mutation into the orthologous position of the mouse Gjc2 gene and inserted the mCx47M282T coding sequence into the mouse genome via homologous recombination in embryonic stem cells. (prolekarniky.cz)
  • The ECFP and EYFP-expressing transgenic ES cells and mice that we have generated provide sources of cells and tissues for combinatorial, double-tagged recombination experiments, chimeras or transplantations. (biomedcentral.com)
  • Lee SL, Thomas P , Fenech M. Extracellular amyloid beta 42 causes necrosis, inhibition of nuclear division, and mitotic disruption under both folate deficient and folate replete conditions as measured by the cytokinesis-block micronucleus cytome assay. (academictree.org)
  • Upon starvation or lysosomal stress, inhibition of MTOR induces TFE3 dephosphorylation, resulting in nuclear localization and transcription factor activity (PubMed:31672913). (neobiotechnologies.com)
  • We determine a comprehensive map of lineage-specific lncRNAs in human dermal lymphatic and blood vascular endothelial cells (LECs and BECs), combining RNA-Seq and CAGE-Seq. (nature.com)
  • To date, a large number of human non-small cell lung cancer cell lines are being used for both basic research and drug discovery. (ubigene.us)
  • A549 cells are well-characterized among the human lung carcinoma/alveolar cell lines as well as having KRAS mutation and wild type EGFR. (ubigene.us)
  • Four of these hybridomas, namely 2A9, 5A7, 7F9 and 8G11, were further confirmed to secrete specific antibodies against Meq as confirmed by the IFA staining of 293T cells overexpressing Meq. (pirbright.ac.uk)
  • Confocal microscopic analysis of cells stained with these antibodies confirmed the nuclear localization of Meq in MDV-infected CEF cells and MDV-transformed MSB-1 cells. (pirbright.ac.uk)
  • Thus, there exists in the leg disc a set of mutually antagonistic interactions between proximal cells, which we define as those that express hth , and distal cells, or those that do not express hth . (biologists.com)
  • Ubigene has modified over 5000 genes from more than 200 cell lines with our exclusive innovation CRISPR-U™ technology. (ubigene.us)
  • To isolate bovine oocyte marker genes, we performed suppressive and subtractive hybridization between oocytes and somatic tissues (i.e., intestine, lung, muscle, and cumulus cells). (appliedbioinfo.com)
  • The subtracted library was characterized by sequencing 185 random clone inserts, representing 146 nonredundant genes. (appliedbioinfo.com)
  • explosion further, consider that a fictitious small genome with 2002) More recently and more dramatically, the potential for 260 genes would host the same number of combinations as cell state conversions is exemplified by the reprogramming of the number of atoms in the visible universe! (lu.se)
  • Understanding cell-fate decisions in stem cell populations is a major goal of modern biology. (lu.se)
  • An RNAi construct selectively driven in cyst cells leads to male sterility, demonstrating the necessity of noa function for male germline development and the interaction of the somatic cyst cells with the developing sperm. (biologists.com)
  • Male germ cell development in Drosophila is dependent on the close contact of the germ cells with the surrounding somatic cyst cells. (biologists.com)
  • A single germ cell, a spermatogonium, becomes enclosed by two somatic cyst cells, thus forming a cyst. (biologists.com)
  • Overexpression of R3052W in DLD1 parental BRCA2 wild-type cells confers sensitivity to MMC DNA damage. (frontiersin.org)
  • Of 768 clone inserts submitted for differential screening by macroarray hybridization, 83% displayed a fourfold overexpression in the oocyte. (appliedbioinfo.com)
  • General Description of TCL1 (T-Cell Marker). (enquirebio.com)
  • enQuire Bio's product, TCL1 (T-Cell Marker) MonoSpecific Antibody, is available for Research Use Only (RUO-Only). (enquirebio.com)
  • This MAb is an excellent marker for all nuclei in cells in tissues. (neobiotechnologies.com)
  • These results show that IL-6-induced fibroblast cooperativity can control the development of a nematic ECM, highlighting both IL6 signalling and cell-cell adhesions as potential therapeutic targets to inhibit this common feature of fibrosis. (bvsalud.org)
  • In PC12 cells and cultured hippocampal neurons, transport activity of GAKIN contributes to the accumulation of PIP 3 at the tip of neurites. (silverchair.com)
  • All MLAs exhibited architectural diversity, compactly aggregated small tubules, eosinophilic intraluminal secretions, overlapped and angulated nuclei, scant cytoplasm, and presence of spindle cells. (iiarjournals.org)
  • A stable AEG‑1‑deficient HT22 neuronal cell line was constructed using CRISPR/Cas9 gene‑editing technology. (spandidos-publications.com)
  • In AST, AEG-1 causes glutamatergic excitotoxicity by downregulating the activity of the excitatory amino acid transporter 2 (EAAT2) promoter, leading to neuronal cell death in glioma-induced neurodegenerative disease ( 10 ). (spandidos-publications.com)
  • Cell growth was sized by MTT assays each day time for 3 days. (techblessing.com)
  • This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. (abcam.com)
  • (C) Clonogenic survival analyses of BRCA2 WT versus the two independent R3052W clones after treatment with Olaparib, cisplatin, and mitomycin C. (D) Schematic of I-SceI nuclease-induced DSB HDR luciferase assay. (frontiersin.org)
  • (G) Clonogenic survival analyses of DLD parental clones upon treatment with mitomycin C. (frontiersin.org)
  • In conclusion, the reduction in IAV copy number in DEFB1 overexpressing cells suggests that beta-defensin-1 plays a key role in regulating IAV survival through STAT3 and is a potential target for antiviral drug development. (cdc.gov)
  • Thereafter, a distinct subpopulation of endothelial cells lining the cardinal vein starts differentiating by expressing the TF PROX1, the master regulator of lymphatic endothelial cell (LEC) identity, via the TFs SOX18 and COUPTFII. (nature.com)
  • MW) chemicals interact with host cells and molecules and In our daily life, we are confronted with a plethora of natural influence biologic processes such as signal transduction. (cdc.gov)
  • These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. (neobiotechnologies.com)