• In vivo vMSOT brain imaging of P301L mice showed higher retention of PBB5 in the tau-laden cortex and hippocampus compared to wild-type mice, as confirmed by ex vivo vMSOT, epi-fluorescence, multiphoton microscopy, and immunofluorescence staining. (springer.com)
  • Immunofluorescence is a technique used for light microscopy with a fluorescence microscope and is used primarily on biological samples. (wikipedia.org)
  • Renal pathology provides on-campus histopathology, immunofluorescence and electron microscopy. (jefferson.edu)
  • The parasites were incubated for 1 or 2 hr in medium ± Trolox, treated with MitoTracker (red) and subjected to immunofluorescence microscopy using antibodies against ( A ) mtTXNPx (green) or ( B ) VDAC (green). (elifesciences.org)
  • Studies in the Frappier laboratory involve a variety of methodologies, including proteomics approaches for protein interactions and host cell manipulations, functional assays in human cells, immunofluorescence microscopy, RNA interference, protein biochemistry and protein structural studies. (utoronto.ca)
  • There are two classes of immunofluorescence techniques, primary (or direct) and secondary (or indirect). (wikipedia.org)
  • Direct immunofluorescence, although somewhat less common, has notable advantages over the secondary (indirect) procedure. (wikipedia.org)
  • Since the number of fluorescent molecules that can be bound to the primary antibody is limited, direct immunofluorescence is substantially less sensitive than indirect immunofluorescence and may result in false negatives. (wikipedia.org)
  • Indirect immunofluorescence experiments were performed to follow these nuclear matrix antigens during apoptosis. (nih.gov)
  • In addition, it was aimed to compare the results of indirect immunofluorescence (IIF) antinuclear antibody (ANA) patterns and extractable nuclear antigen (ENA) antibodies. (unboundmedicine.com)
  • ANA, anti-mitochondrial antibody (AMA)/anti-liver-kidney microsomal antibody (LKM), anti-smooth muscle antibody (ASMA), antineutrophil cytoplasmic antibody (ANCA) assays were performed by indirect immunofluorescence method (IIF) (Euroimmune AG, Luebeck, Germany). (unboundmedicine.com)
  • Procedures are provided for perturbation of cells using siRNA and reliable methodology for marker detection by indirect immunofluorescence in 96-well formats. (jove.com)
  • The determination of autoantibody titers from 1:80 to 1:1280 was performed by serial dilution on samples that showed a 3+ or greater nuclear and/or cytoplasmic immunofluorescence pattern. (cdc.gov)
  • Following TDP-43 sequestration in insoluble aggregates, we observed higher nuclear levels of EIF4A3 and POLDIP3β, whereas nuclear levels of DNMT3A, HNRNPA3, PABPC1 and POLDIP3α dropped and cytoplasmic levels of RANBP1 dropped. (nature.com)
  • In addition, immunofluorescence signal intensity quantifications showed increased nuclear expression of HNRNPL and YARS and downregulation of cytoplasmic DPCD. (nature.com)
  • Furthermore, cytoplasmic levels of predominantly nuclear protein ALYREF increased. (nature.com)
  • The workflow quantifies nuclear and cytoplasmic markers and computes marker translocation between these compartments. (jove.com)
  • Here we report a class of mRNA granules in human neuronal processes that are enriched in the nuclear cap-binding protein complex (CBC) and exon junction complex (EJC) core components, Y14 and eIF4AIII. (frontiersin.org)
  • The existence of mRNAs retaining both nuclear cap binding protein and EJC in the distal sites of neuronal processes suggests that some localized mRNAs have not yet undergone the "very first translation," which contribute to the spatio-temporal regulation of gene expression. (frontiersin.org)
  • We measured an extensive panel of autoantibodies (including rheumatoid factor, anti-cyclic citrullinated peptide, and anti-nuclear antibodies by immunofluorescence) associated with connective tissue disease or vasculitis in a cohort of well-characterized patients with idiopathic pulmonary fibrosis (n = 67). (nih.gov)
  • Immunofluorescence analysis indicated that TNF‑α increased p65 translocation from the cytoplasm to the nucleus. (spandidos-publications.com)
  • To obtain immune receptor data from the cell surface and cytoplasm after nuclear capture, a "contour" is generated at a fixed distance from the nuclear perimeter. (ersjournals.com)
  • Testing of IgG autoantibodies to human cellular antigens was performed by the HEp-2 cell immunofluorescence assay using slides from INOVA Diagnostics, San Diego, CA (Cat # 508100) following the manufacturer's instructions and evaluated primarily using their NOVA View system. (cdc.gov)
  • While the antibody to the nucleolar isoform of DNA topoisomerase II gave a fluorescent pattern that was well-maintained until the late phases of apoptosis, the other three nuclear antigens showed marked modifications in their distribution. (nih.gov)
  • Antinuclear antibody patterns at immunofluorescence, such as multiple nuclear dot and rim-like patterns [ 14 ] corresponding to gp210 and sp100 antigens on enzyme-linked immunosorbent assay, [ 9 ] may be helpful for diagnosis in AMA-negative patients, given their specificity for PBC. (medscape.com)
  • Immunofluorescence can be used on tissue sections, cultured cell lines, or individual cells, and may be used to analyze the distribution of proteins, glycans, and small biological and non-biological molecules. (wikipedia.org)
  • If the topology of a cell membrane has yet to be determined, epitope insertion into proteins can be used in conjunction with immunofluorescence to determine structures. (wikipedia.org)
  • In this study we focused our attention on the behavior of four nuclear matrix proteins during the various stages of apoptosis in the HL-60 cell line exposed to the DNA topoisomerase I inhibitor, camptothecin. (nih.gov)
  • These characteristics correlate with a distinct nuclear architecture, epigenetic signatures enriched for active chromatin marks and hyperdynamic binding of structural chromatin proteins. (biomedcentral.com)
  • The accuracy of mitochondrial DNA (mtDNA) replication depends on the coordinated action of many nuclear-encoded proteins and on the correct balance of nucleotides within the mitochondrial matrix. (novusbio.com)
  • These studies include identifying proteins that affect PML nuclear bodies, cell cycle progression and DNA damage responses. (utoronto.ca)
  • Fourteen of the patients (10.6%) with centromere, nuclear membrane (NM), multiple nuclear dot (MND) staining patterns and elevated liver enzymes could not be diagnosed as a specific disease and were followed up. (unboundmedicine.com)
  • TA muscle was homogenized and prepared for cell death enzyme-linked immunosorbent assay (ELISA), while histomorphology was conducted on transverse sections of the TA muscle mid-belly prepared for terminal dUTP nick-end labeling (TUNEL) and laminin duel immunofluorescence and quantified via standard stereology. (cdc.gov)
  • Immunofluorescence can be used in combination with other, non-antibody methods of fluorescent staining, for example, use of DAPI to label DNA. (wikipedia.org)
  • Blue: DAPI for nuclear staining. (novusbio.com)
  • Nuclear and kinetoplast DNA were visualized by DAPI staining (blue). (elifesciences.org)
  • The nuclear stain is DAPI (blue). (everestbiotech.com)
  • NF-κB is a nuclear transcription factor that participates in several physiopathological processes, such as apoptosis, inflammation and various autoimmune diseases ( 8 , 9 ). (spandidos-publications.com)
  • In addition, we found that EBNA1 disrupts the host PML nuclear bodies required for apoptosis and DNA repair, thereby promoting cell survival with DNA damage. (utoronto.ca)
  • Increased skeletal muscle nuclear apoptosis has been suggested to be involved with age-related pathological remodeling that contributes to sarcopenic states. (cdc.gov)
  • Nuclear Magnetic Resonance and release kinetics assessed the rate of polymer degradation. (aiche.org)
  • Primary (direct) immunofluorescence uses a single, primary antibody, chemically linked to a fluorophore. (wikipedia.org)
  • Direct immunofluorescence also requires the use of much more primary antibody, which is extremely expensive, sometimes running up to $400.00/mL. (wikipedia.org)
  • To identify a nuclear marker pathognomonic of astrocytic phenotype, we assessed differential RNA expression by FACS-purified adult astrocytes and, on that basis, evaluated the expression of the transcription factor SOX9 in both mouse and human brain. (jneurosci.org)
  • We show here that SOX9 is an astrocyte-specific nuclear marker in all major areas of the CNS outside of the neurogenic regions. (jneurosci.org)
  • Immunofluorescence can also be used as a "semi-quantitative" method to gain insight into the levels and localization patterns of DNA methylation since it is a more time-consuming method than true quantitative methods and there is some subjectivity in the analysis of the levels of methylation. (wikipedia.org)
  • EB06619 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. (everestbiotech.com)
  • red) at a 1:100 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). (sigmaaldrich.com)
  • This term describes a common type of CSVV, so-called because inflammation consists initially of neutrophils, which after degranulation result in deposition of nuclear debris (leukocytoclasis) in the vessel wall. (msdmanuals.com)
  • In latency EBV genomes are maintained as circular nuclear episomes that replicate once-per-cell cycle and are partitioned to the daughter cells during mitosis. (utoronto.ca)
  • Initially described in Japan, KFD was first reported almost simultaneously by Kikuchi and by Fujimoto and associates in 1972 as a lymphadenitis with focal proliferation of reticular cells accompanied by numerous histiocytes and extensive nuclear debris. (medscape.com)
  • These results show that, in addition to the well-known chromatin changes, nuclear organization undergoes other rearrangements during the apoptotic process. (nih.gov)
  • BRCA2 nuclear localization and export sequences are listed. (frontiersin.org)
  • Androgen receptor (AR) also konwn as Dihydrotestosterone receptor (DHTR), Nuclear receptor subfamily 3 group C member 4 (NR3C4).It is one of steriod hormoen receptors, which are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. (ptglab.com)
  • In addition to the element to which fluorescence probes are attached, there are two general classes of immunofluorescence techniques: primary and secondary. (wikipedia.org)
  • In addition, a range of immunofluorescence parameters were generated for CD68. (ersjournals.com)
  • The U1 small nuclear ribonucleoprotein particle (snRNP) is a target of autoreactive B cells and T cells in MCTD. (medscape.com)
  • To investigate the involvement of stem cells in Epstein-Barr virus infection-related nasopharyngeal carcinoma (NPC), we used double immunofluorescence staining to examine several cancer stem/progenitor cell markers (CD44v6, CD24, and ALDH1A1) in NPC tissues and NPC cell lines. (hindawi.com)
  • Nuclear dust is often apparent, and a variable number of eosinophils and mononuclear cells are present within the dermal infiltrate. (medscape.com)
  • Not only did curcumin treatment increase mitochondrial DNA (mtDNA) copy number in hepatocytes, but it also increased levels of transcriptional factors that regulate mitochondrial biogenesis, including peroxisome proliferator-activated receptor-γ coactivator 1α (PGC1α), nuclear respiratory factor 1 (NRF1) and mitochondrial transcription factor A (Tfam). (spandidos-publications.com)
  • Taken together, aggregation of TDP-43 is most probably the root cause of ALS/FTLD either through a gain of toxic function (GOF) on its own or through a loss of function (LOF) with sequestration and depletion of nuclear TDP-43 23 , 24 or both 25 . (nature.com)