Nuclear fractionation analysis revealed nuclear proteins. Medical search. Frequent questions

Using both biochemical fractionation and immunoelectron microscopy methods, these vesicles were shown to contain nups. (tamu.edu)
Remarkably, biochemical fractionation exposed that a majority of METTL16 protein resides in the cytoplasm of a number of cells. (diferencias-entre.org)
Biochemical fractionation as well as confocal microscopy studies revealed that KBrO3-induced BER foci are completely excluded from heterochromatin rich regions, and are preferentially located in less-condensed DNA areas where active transcription takes place. (cea.fr)
Microscopy, biochemical fractionation and chromatin immunoprecipitation reveal a diffuse nucleoplasmic distribution, weak association with chromatin and high expression levels for HP1β in ESCs. (biomedcentral.com)
The S. cerevisiae Mdm12p was localized by indirect immunofluorescence microscopy and by subcellular fractionation and immunodetection to the mitochondrial outer membrane and displayed biochemical properties of an integral membrane protein. (rupress.org)

Subcellular localization critically influences protein function, and cells control protein localization to regulate biological processes. (elifesciences.org)
We initially used maps statically to generate a database with localization and absolute copy number information for over 8700 proteins from HeLa cells, approaching comprehensive coverage. (elifesciences.org)
Protein function is critically determined by subcellular localization, as organelles offer different chemical environments and interaction partners. (elifesciences.org)
In order to regulate protein activity, many biological processes involve changes in protein subcellular localization. (elifesciences.org)
2 Protein interactions are critical to the stability, localization and activity of b-catenin, and we recently performed the first proteomic analyses of the b-catenin interactome in myeloid cells. (haematologica.org)
Recently, it was revealed that the ordered localization of proteins and nucleic acids in the membraneless intracellular microenvironment is modulated by liquid-liquid phase separation (LLPS) ( Shin and Brangwynne, 2017 ). (aspetjournals.org)
Interestingly, exogenously overexpressed METTL16 differed from your endogenous protein in its relative affinity for RNA focuses on which prompted us to investigate METTL16's localization within the cell. (diferencias-entre.org)
Therefore, while METTL16 has been reported to be a nuclear protein, our findings suggest that METTL16 is also a cytoplasmic methyltransferase that may alter its RNA binding Niraparib hydrochloride preferences depending on its cellular localization. (diferencias-entre.org)
Genetic mutations in ATRX, DAXX, and H3.3 have been detected in ALT positive cancers, however, a subset of ALT samples show loss of ATRX or DAXX protein expression or localization without evidence of genetic alterations suggesting additional uncharacterized defects in ATRX/DAXX/H3.3 function. (oncotarget.com)
A beta-hairpin comprising the nuclear localization sequence sustains the self-associated states of nucleosome assembly protein 1. (colorado.edu)
Furthermore, the deleterious aftereffect of missense mutations can occur not merely from immediate disruption of CAPN3 function(s), but additionally from disruption from the protein's structural integrity, that could affect its intra or intermolecular protein lead and interactions to decreased stability or altered localization. (exposed-skin-care.net)

Deep RNA-DNA, RNA-protein interaction studies, and phenotype rescue analyses reveal that LETR1 is a nuclear trans-acting lncRNA modulating, via key epigenetic factors, the expression of essential target genes, including KLF4 and SEMA3C , governing the growth and migratory ability of LECs. (nature.com)
Four mutant complementation groups were characterized that correspond to respective mutations in genes encoding Ran (gsp1), and essential Ran regulatory factors Ran GTPase-activating protein (rna1), Ran guanine nucleotide exchange factor (prp20), and the RanGDP import factor (ntf2). (tamu.edu)
120 different translocations, resulting in the generation of MLL-fusion proteins encompassing more than 75 different partner genes 5 . (nature.com)
In some genes these proteins add a poly(A) tail at one of several possible sites. (wikipedia.org)
It is a main inductor of genes of antioxidant proteins and phase II detoxifying enzymes [ 1 ]. (hindawi.com)
The conserved family of Transcription Intermediary Factors (TIF1) proteins consists of key transcriptional regulators that control transcription of target genes by modulating chromatin state. (elifesciences.org)
DDX5 is required for cell proliferation by controlling the transcription of genes expressing DNA replication proteins in cancer cells in which the DDX5 locus is amplified, and this has uncovered a dependence on DDX5 for cell proliferation. (aacrjournals.org)
As opposed to other styles of muscles dystrophy, where mutations take place in genes encoding structural protein, calpainopathy was the initial reported kind of dystrophy predetermined by mutations within a gene encoding a proteolytic enzyme. (exposed-skin-care.net)

The defects did not affect the stability of existing NPCs, and nup mislocalization was dependent on de novo protein synthesis and continued cell growth. (tamu.edu)
Messenger RNA (mRNA) is RNA that has a coding region that acts as a template for protein synthesis (translation). (wikipedia.org)
In organello mitochondrial protein and RNA synthesis systems from Saccharomyces cerevisiae. (colorado.edu)
MSY3 (MSY4, YB-2, CSDA, dbpA, ZONAB) is a member of the cold shock domain (CSD) family of proteins also known as Y-box proteins, which are evolutionarily conserved proteins that function as transcription factors and regulators of RNA metabolism and protein synthesis. (biomedcentral.com)

Here we used subcellular fractionation and immunofluorescence to understand such complex functions of UPF1. (molcells.org)
Subcellular fractionation further indicated that GAPDH remained relatively constant in the cytosol, while increasing at 12 to 24 hours postinfection (hpi) and decreasing at 36 hpi in the nuclear fraction of infected cells. (biomedcentral.com)

3 This study identified Wilms tumor protein (WT1) as a putative novel interaction partner in myeloid cells. (haematologica.org)
WT1 is an RNA-binding protein, 6 and β-catenin has also been shown to bind RNA, 7 so to confirm this interaction was not indirect via RNA binding we repeated WT1 Co-IP (with or without CHIR99021) after first confirming complete digestion of RNA through RNase A pre-treatment of cell lysates ( Figure 1E ). (haematologica.org)
We further wanted to ascertain whether this protein interaction was direct using recombinant versions of purified b-catenin and WT1 protein but failed to detect any association ( Online Supplementary Figure S1B, C ). This suggested that perhaps the interaction is mediated through a post-translational modification, common partners such as WTX, 8 or cellular structures like DNA, given their well-documented roles in transcription. (haematologica.org)
In our recent study, it was revealed that PXR interacts with DAZ-associated protein 1 (DAZAP1), which is an essential component of the paraspeckle, a membraneless nuclear body, and the interaction was disassociated by rifampicin, a ligand of PXR. (aspetjournals.org)
Immunoprecipitation assays using PXR-overexpressing HepG2 (ShP51) cells revealed that PXR interacts with not only DAZAP1 but also NEAT1_2, a long noncoding RNA included in the paraspeckle, and that the interaction between PXR and NEAT1_2 was disassociated by rifampicin. (aspetjournals.org)
SIGNIFICANCE STATEMENT This study revealed that PXR interacts with paraspeckle components NEAT1_2 and DAZAP1 to suppress CYP3A4 induction by PXR, and the interaction is dissociated by PXR ligands. (aspetjournals.org)
Through this interaction, mRNAs and their associative proteins form messenger ribonucleoprotein particles (mRNPs) that are actively transported along the cytoskeleton to intracellular destinations. (frontiersin.org)
In order to obtain more detailed information regarding the interaction between host proteins and the defined viral nucleic acid and/or proteins, we investigated the host factors associating with the main replicase enzyme, NS5, in the JEV-infected cells using yeast-two hybrid screening. (biomedcentral.com)
We evaluated a next generation therapeutic peptide, HTL-001, capable of targeting HOX gene over-expression in GBM by disrupting the interaction between HOX proteins and their co-factor, PBX. (biomedcentral.com)
Balusu R, Jaiswal AS, Armas ML, Kundu CN, Bloom LB, Narayan S. Structure/function analysis of the interaction of adenomatous polyposis coli with DNA polymerase beta and its implications for base excision repair. (famri.org)

Using a recombinant endonuclease-deficient Cas9-based gene promoter pulldown approach coupled with mass spectrometry, we found that MCUb is upregulated in the T2D heart due to loss of glucose homeostasis regulator nuclear receptor corepressor 2 repression, and chromatin immunoprecipitation assays identified peroxisome proliferator-activated receptor α as a mediator of MCUb gene expression in T2D cardiomyocytes. (diabetesjournals.org)

Solid evidence, leveraging both in vivo and in vitro studies, reveals the molecular requirements for Bonus' function as a transcriptional repressor, including a key role for sumoylation. (elifesciences.org)
Gene therapy displacement of endogenous MCUb with a dominant-negative MCUb transgene (MCUb W246R/V251E ) in vivo rescued T2D cardiomyocytes from metabolic inflexibility and stimulated cardiac contractile function and adrenergic responsiveness by enhancing phospholamban phosphorylation via protein kinase A. We conclude that MCUb represents one newly discovered molecular effector at the interface of metabolism and cardiac function, and its repression improves the outcome of the chronically stressed diabetic heart. (diabetesjournals.org)
Here, we analyzed the protein levels, activity and role of Rsk in in vivo and in vitro HD models. (biomedcentral.com)
Aim: We aimed to investigate the expression of monocyte chemoattractant proteins (MCPs) and their cognate receptor CC chemokine receptor 2 (CCR2) in aortas of apolipoprotein E-null (apoE -/- ) mice during atherogenesis as well as the possible transcription pathway involved in the early induction of MCP-1 in vascular smooth muscle cells (VSMCs) in vivo . (go.jp)

Human pregnane X receptor (PXR) is a major nuclear receptor that upregulates the expression of drug-metabolizing enzymes such as CYP3A4. (aspetjournals.org)
Kidney mitochondria showed coordinate induction of tricarboxylic acid (TCA) cycle enzymes, whereas TCA cycle proteins were repressed in cardiac mitochondria. (omicsdi.org)

Electron microscopy analysis revealed striking membrane perturbations and the accumulation of vesicles in arrested mutants. (tamu.edu)
We obtained more direct evidence through generating improved transmission electron microscopy (TEM) images and image averages of the rosette-type CSC, revealing the frequent triangularity and average cross-sectional area in the plasma membrane of its individual lobes. (osti.gov)

Many RNA binding proteins (RBPs) including the YTH family of proteins modulate the effects of m6A through specific binding to the methylated RNA. (diferencias-entre.org)

1) Characterize novel families of oncoproteins (Pbx and Meis) that dimerize with and regulate the DNA binding properties of Hox proteins. (stanford.edu)
We are investigating how Pbx and Meis proteins contribute to the specificity of Hox function in development and how disruption of their activities leads to neoplasia. (stanford.edu)

We assessed the association of PRSs with COPD and asthma using modified random-effects and binary-effects meta-analyses, and ACO and asthma exacerbations in specific cohorts. (bvsalud.org)
According to many prospective cohort studies and meta-analyses of those studies, physical inactivity and/or low levels of physical fitness are associated with an elevated risk for the development of metabolic syndrome, type 2 diabetes, hypertension, coronary artery disease (CAD), and stroke, and with an increased risk of cardiovascular disease (CVD) mortality and all-cause mortality. (go.jp)

We present the first comprehensive survey of protein-protein interactions of seven distantly related MLL-fusion proteins. (nature.com)
Families of related leukemia fusion proteins that share genomic and biological properties represent unique opportunities to study how the combination of distinct functional protein modules can drive oncogenic transformation. (nature.com)
MLL-fusion proteins are found in acute lymphoblastic leukemia (ALL) and acute myeloid leukemia (AML) and are often associated with adverse prognosis, particularly in pediatric patients 3 . (nature.com)
It has therefore been proposed that the oncogenic activity of MLL-fusion proteins depends on chromatin targeting functions exerted by the MLL N-terminus in combination with other functional properties encoded by the fusion partners 6 . (nature.com)
For instance, the CxxC-domain is essential for DNA binding of MLL-fusion proteins 7 . (nature.com)
Furthermore, the MLL-interacting protein Menin links MLL-fusion proteins with LEDGF, and the H3K36me3-binding PWWP domain of LEDGF is critical for the function of MLL-fusions 8 . (nature.com)
However, it is unclear whether these molecular mechanisms pertain to the entire family of MLL-fusions or if they specifically affect the leukemogenicity of isolated MLL-fusion proteins. (nature.com)
HeLa cells expressing the fusion proteins OGG1-DsRED (red) and XRCC1-GFP (green) were treated with KBrO3, that mainly induces 8-oxoG. (cea.fr)

nevertheless, recent studies have got exposed a fresh potential function for CAPN3 being a structural proteins (23,24). (exposed-skin-care.net)

Here we show by mass spectrometric analyses of enriched insoluble protein fractions that, unexpectedly, nucleus-encoded transporters are not inserted into the chromatophore inner envelope membrane. (frontiersin.org)
Three hours after the treatment, BER proteins become insoluble and associate to those regions in which DNA (stained with DAPI, blue) is less condensed, corresponding to euchromatin regions. (cea.fr)

While the precise mechanism underlying activation of the ALT pathway is unclear, mutations in the chromatin remodeling protein ATRX, histone chaperone DAXX, and the histone variant H3.3 correlate with ALT status. (oncotarget.com)
Results from a high throughput siRNA screening suggest that proteins involved in chromatin remodelling, transcription regulation, and histone modifications have a role in the relocalisation of BER proteins to euchromatin regions. (cea.fr)

We are studying the effects and consequences of protein fusion on the transcriptional and transforming activities of these proteins using in vitro and animal models. (stanford.edu)

The poly(A) tail is important for the nuclear export, translation and stability of mRNA. (wikipedia.org)
Here we report a class of mRNA granules in human neuronal processes that are enriched in the nuclear cap-binding protein complex (CBC) and exon junction complex (EJC) core components, Y14 and eIF4AIII. (frontiersin.org)
Methyltransferase like -3 and -14 (METTL3 and METTL14) and Wilms tumor associating protein (WTAP) in addition to KIAA1429 are all components of the mRNA m6A methyltransferase complex, which uses a S-adenosyl methionine (SAM) binding website on METTL3 to methylate specific mRNAs for methylation having a RRACH m6A consensus sequence [10C15]. (diferencias-entre.org)
Analysis from the Gene Expression Omnibus datasets supported the mRNA findings. (hindawi.com)

These results suggest that paraspeckles play a role in trapping nuclear PXR in the absence of the ligand to negatively regulate transactivation of its downstream gene. (aspetjournals.org)
Recently, several chromatin-related proteins have been shown to regulate ESC pluripotency and/or differentiation, yet the role of the major heterochromatin proteins in pluripotency is unknown. (biomedcentral.com)

... [Show full abstract] absence of amino acids, proteins and acidic compounds.Total phenol and flavonoid content were evaluated for all the extracts of both plants. (researchgate.net)

The resulting IL-pretreated switchgrass (PSG) samples were characterized by enzymatic hydrolysis, cross polarization/magic angle spinning (CP/MAS) C-13 nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FTIR), and 2D NMR spectroscopy. (metu.edu.tr)

Bae KM, Wang H, Jiang G, Chen MG, Lu L, Xiao L. Protein kinase C epsilon is overexpressed in primary human non-small cell lung cancers and functionally required for proliferation of non-small cell lung cancer cells in a p21/Cip1-dependent manner. (famri.org)
Chemical genetics reveals the requirement for Polo-like kinase 1 activity in positioning RhoA and triggering cytokinesis in human cells. (famri.org)
Rsk activation is regulated by sequential phosphorylations controlled by extracellular signal-regulated kinase (ERK) 1/2 and 3-phosphoinositide-dependent protein kinase 1 (PDK1). (biomedcentral.com)

In eukaryotic cells, various proteins and nucleic acids are orderly arranged in the nucleus and cytoplasm ( Handwerger and Gall, 2006 ). (aspetjournals.org)
It imports hundreds of nucleus-encoded proteins, and diverse metabolites are continuously exchanged across the two chromatophore envelope membranes. (frontiersin.org)
Furthermore, chromatophore-localized biosynthetic pathways as well as multiprotein complexes include proteins of dual genetic origin, suggesting that mechanisms evolved that coordinate gene expression levels between chromatophore and nucleus. (frontiersin.org)
Interestingly, all the proteins involved in BER are encoded by the nuclear genome and the mechanism responsible for the targeting of the translated protein to the nucleus or to the mitochondria have not been clearly established yet. (cea.fr)
We observed that, as skeletal muscle progressed from perinatal to postnatal and adult developmental stages, MSY3 protein became gradually dephosphorylated and accumulated in the nucleus. (biomedcentral.com)
The increase of Rsk in STHdh Q111/Q111 cells occurred in the cytosol and in the nucleus, which results in enhanced phosphorylation of both cytosolic and nuclear Rsk targets. (biomedcentral.com)
1 nucleus with a static residual quadrupole coupling, also for the water-1H magnetization, even in systems with high enhanced I-spin relaxation is observed at I-spin Larmor frequencies water content, as long as the protein component is immobilized matching an eigenfrequency of the static S-spin Hamiltonian. (lu.se)

The analysis showed great genetic diversity of the different strains between countries and regions. (cdc.gov)
Sequencing and other techniques may reveal the actual genetic defects in these patients. (medscape.com)

These findings imply that similar to the situation in mitochondria and plastids, also in P. chromatophora nuclear factors evolved that control metabolite exchange and gene expression in the chromatophore. (frontiersin.org)
Mitochondria are stainied with MitoTracker Deep Red (magenta), and the nuclear DNA is visualized by DAPI staining (blue). (cea.fr)
In diabetic mice, fatty acid oxidation (FAO) proteins were less abundant in liver mitochondria, whereas FAO protein content was induced in mitochondria from all other tissues. (omicsdi.org)

Two other proteins add specificity to the binding to an RNA: CstF and CFI. (wikipedia.org)

These characteristics correlate with a distinct nuclear architecture, epigenetic signatures enriched for active chromatin marks and hyperdynamic binding of structural chromatin proteins. (biomedcentral.com)

Characterization of DNA binding, transcriptional activation, and regulated nuclear association of recombinant human NFATp. (colorado.edu)
The recombinant protein, overexpressed in Escherichia coli, exhibits a low catalytic activity and an unexpectedly high thermal stability (Tm = 64.8 degrees C). Based on various spectroscopic data, on the known three-dimensional structure of the AK from E. coli and on secondary structure predictions for various sequenced AKs, we propose a structural model for AK from M. tuberculosis (AKmt). (rhea-db.org)

Mdm12p is the third mitochondrial outer membrane protein required for normal mitochondrial morphology and distribution to be identified in S. cerevisiae and the first such mitochondrial component that is conserved between two different species. (rupress.org)
The molecular mechanisms and cellular components that mediate this mitochondrial inheritance are beginning to be elucidated through the analysis of Saccharomyces cerevisiae mutants exhibiting specific defects in mitochondrial distribution. (rupress.org)
Pressure overload cardiac hypertrophy, a risk factor for heart failure, is associated with reduced mitochondrial fatty acid oxidation (FAO) and oxidative phosphorylation (OXPHOS) proteins that correlate in rodents with reduced PGC-1? (omicsdi.org)

We have developed and applied Dynamic Organellar Maps, a proteomic method that allows global mapping of protein translocation events. (elifesciences.org)

These are frequently activated by fusion to other transcriptional proteins resulting in chimeric transcription factors. (stanford.edu)
Nuclear factor erythroid-2-related factor 2 (NRF2) is the most studied member of the Cap 'n' collar basic leucine zipper (CNC-bZIP) family of transcription factors. (hindawi.com)

After analysing for many years the way that DNA glycosylases, APE1 and XRCC1 initiate the repair of 8-oxoG or abasic sites, our laboratory is now interested in studying the effect on BER proteins of cellular environmental changes such as oxidative stress or the switch from stem cells to differentiation. (cea.fr)
deficient mice exhibited greater degrees of oxidative stress, decreased cardiac efficiency, lower rates of glucose metabolism, and repression of hexokinase II protein.PGC-1? (omicsdi.org)

Here, using Next Generation Sequencing we identified a novel gene fusion event between DAXX and the kinesin motor protein, KIFC3, leading to the translation of a chimeric DAXX-KIFC3 fusion protein. (oncotarget.com)

In vertebrates this subfamily contains four proteins: TIF1α/TRIM24, TIF1β/TRIM28, TIF1γ/TRIM33, and TIF1δ/TRIM66, while only one protein, Bonus (Bon), is present in Drosophila , making it an attractive model to understand the conserved functions of TIF1 proteins. (elifesciences.org)

The PSG samples derived from recycled IL were rapidly hydrolyzed, and a high glucan digestibility of 80 % was obtained even at a low enzyme loading of 5 mg protein g(-1) glucan. (metu.edu.tr)

Heterogeneous population of RNA granules serve as motile units to translocate, store, translate, and degrade mRNAs in the dendrites contain cis -elements and trans -acting factors such as RNA-binding proteins and microRNAs to convey stimulus-, transcript-specific local translation. (frontiersin.org)
viral proteins may decoy some host factors for assisting viral replication. (biomedcentral.com)
Two critical factors during this dauntingly complex process appear to be the establishment of metabolic connectivity between the symbiotic partners, and the evolution of nuclear control over protein expression levels within the organelle. (frontiersin.org)
More recently we have also established that unexpected factors such as the cellular prion protein can stimulate BER and we are now trying to identify the underlying mechanisms and the possible physiological implications of this response. (cea.fr)

Analysis of the phosphorylation of Rsk in Hdh mice and STHdh cells showed reduced levels of phospho Ser-380 (dependent on ERK1/2), whereas phosphorylation at Ser-221 (dependent on PDK1) was increased. (biomedcentral.com)
When activated, Rsk promotes the phosphorylation of many cytosolic and nuclear targets. (biomedcentral.com)

Recent studies have revealed the importance of long noncoding RNAs (lncRNAs) as tissue-specific regulators of gene expression. (nature.com)

Hypoxia of CEC was induced by exposing the cells to cobalt chloride (Sigma-Aldrich, St. Louis MO) in serum media, as described previously [ 12 , 13 ], and the induction was confirmed by cytotoxicity and cell cycle analysis. (molvis.org)

These data highlight a potentially unrecognized mechanism of DAXX inactivation in ALT positive osteosarcoma and provide rationale for thorough and comprehensive analyses of ATRX/DAXX/H3.3 proteins in ALT positive cancers. (oncotarget.com)
Different mechanism including alternative splicing, alternative transcription start or dual initiation of translation, have been proposed for different BER proteins. (cea.fr)
Finally, we showed that Bonus SUMOylation is mediated by the SUMO E3-ligase Su(var)2-10, revealing that although SUMOylation of TIF1 proteins is conserved between insects and mammals, both the mechanism and specific site of modification is different in the two taxa. (elifesciences.org)
For biological systems, Q peaks were first observed in protein from different perspectives [3,7,9,13,15,21-23] , the detailed molec- powders and attributed to 1H-14N spin pairs [9], which were also ular mechanism of the ubiquitous 1H-14N Q peaks in biological sys- thought to be of critical importance for the background 1H relaxa- tems has not been established. (lu.se)

All the mutants showed temperature-dependent mislocalization of green fluorescence protein (GFP)-tagged nucleoporins (nups) and the pore-membrane protein Pom152. (tamu.edu)

Numerous studies have shown that replication of RNA viruses is involved in many specific RNA-RNA, RNA-protein, and protein-protein interactions. (biomedcentral.com)

The prognostic significance of the major redox regulator nuclear factor erythroid-2-related factor (NRF2) is recognized in many cancers, but the role of NRF1 is not generally well understood in cancer. (hindawi.com)

FoxO1 acetylation levels and binding to SirT1 were studied by Western immunoblot analysis in INS832/13 cells. (diabetesjournals.org)
The role of SIRT1 in the activation of HIF-2α and nuclear factor-κB (RelA/p65) during hypoxia in the presence or absence of SIRT1 was assessed using immunoblot analysis. (molvis.org)

At the These samples would be pooled and processed by using outset, researchers wanted to determine whether viruses as available technology to isolate virus particles en masse, a class differed in a systematic way from all other small recover viral nucleic acids, produce amplified shotgun particles in nature. (cdc.gov)

While studies of selected MLL-fusions have revealed critical molecular pathways, unifying mechanisms across all MLL-fusions remain poorly understood. (nature.com)

In particular, the FANTOM (Functional Annotation of the Mammalian Genome) consortium pioneered the discovery of the noncoding RNA world by providing, through Cap Analysis of Gene Expression (CAGE-Seq), the first evidence that large portions of our genome are transcribed, producing a multitude of sense and antisense transcripts 12 . (nature.com)
The existence of mRNAs retaining both nuclear cap binding protein and EJC in the distal sites of neuronal processes suggests that some localized mRNAs have not yet undergone the "very first translation," which contribute to the spatio-temporal regulation of gene expression. (frontiersin.org)
Apparently these chromatophore-targeted proteins evolved convergently to plastid-targeted expression regulators and are likely involved in gene expression control in the chromatophore. (frontiersin.org)
Together, our work identified Bonus as a regulator of tissue-specific gene expression and revealed the importance of SUMOylation as a regulator of complex formation in the context of transcriptional repression. (elifesciences.org)

2002. The accidental sinking of the nuclear submarine, the Kursk: monitoring of radioactivity and the preliminary assessment of the potential impact of radioactive releases. (cdc.gov)

These proteins show similar domain architectures as known organelle-targeted expression regulators of the octotrico peptide repeat type in algae and plants. (frontiersin.org)

Combining spatial and abundance information yielded an unprecedented quantitative view of HeLa cell anatomy and organellar composition, at the protein level. (elifesciences.org)

The location of many proteins in a cell is unclear or simply not known. (elifesciences.org)
SIGNIFICANCE STATEMENT Although the loss of several synaptic proteins has been described in Alzheimer's disease (AD), it remains unclear whether their reduction contributes to clinical symptoms. (jneurosci.org)

Understanding how DNA replication is regulated in human cells can provide insight into cancer development and may reveal vulnerabilities that can be exploited therapeutically. (aacrjournals.org)

A decrease in GFP fluorescence associated with the nuclear envelope was observed along with an increase in the diffuse, cytoplasmic signal with GFP foci. (tamu.edu)
Future studies will seek to confirm variations between cytoplasmic and nuclear RNA Niraparib hydrochloride focuses on in addition to exploring the physiological part of METTL16 through long-term knockdown. (diferencias-entre.org)

This analysis, which marks 22 years since the publication of the first Typhi genome, represents the largest Typhi genome sequence collection to date (n=13,000). (cdc.gov)
METHODS: This is a meta-analysis of global genotype and antimicrobial resistance (AMR) determinants extracted from previously sequenced genome data and analysed using consistent methods implemented in open analysis platforms GenoTyphi and Pathogenwatch. (cdc.gov)

Y-box proteins act via diverse mechanisms, as they work by DNA binding and non-DNA binding mechanisms. (biomedcentral.com)

Mechanistically, AFAP1L1 functions as a hypoxia-related regulatory protein that can be activated by HIF-1α. (biomedcentral.com)
In conclusion, AFAP1L1, a newly identified hypoxia-related regulatory protein, can be activated by HIF-1α. (biomedcentral.com)

Based on such different targets of UPF1, this protein is likely to play more comprehensive roles in RNA metabolism than previously known. (molcells.org)

Here we identify Heterochromatin Protein 1β (HP1β) as an essential protein for proper differentiation, and, unexpectedly, for the maintenance of pluripotency in ESCs. (biomedcentral.com)

MSY3 is a DNA binding protein, which acts as a transcriptional repressor. (biomedcentral.com)

We hypothesize that these proteins are involved in modulating membrane permeability. (frontiersin.org)

Anatomy17

Organisms5

Chemicals and Drugs65

Analytical, Diagnostic and Therapeutic Techniques and Equipment34

Phenomena and Processes44

Disciplines and Occupations2

Information Science3

Health Care1

Biochemical5

Localization11

Genes8

Synthesis4

Subcellular fractionation2

Interaction10

Chromatin immunoprecipitation1

VIVO4

Enzymes2

Microscopy2

Family of proteins1

Meis2

Meta-analyses2

Fusion proteins8

Structural proteins1

Insoluble2

Histone2

Vitro1

MRNA4

Regulate2

Abstract1

Magnetic resonance1

Kinase3

Nucleus7

Genetic2

Mitochondria3

Specificity1

Epigenetic1

Recombinant2

Mitochondrial3

Proteomic1

Transcription factors2

Oxidative2

Chimeric1

Subfamily1

Enzyme1

Factors4

Phosphorylation2

RNAs1

Serum1

Mechanism4

Mutants1

Interactions1

Regulator nuclear1

Immunoblot analysis2

Particles1

Pathways1

Gene Expression4

20021

Regulators1

HeLa1

Unclear2

Replication1

Cytoplasmic2

Genome2

Mechanisms1

Regulatory2

Roles1

Unexpectedly1

Transcriptional repressor1

Membrane1