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  • proteins
  • Using the MosSCI technique, we have created Caenorhabditis elegans strains containing single copy insertions of Dam fused to NE and nuclear pore proteins such as emerin/EMR-1, lamin/LMN-1 and Nup98/NPP-10N. (csic.es)
  • Employing a genetically amenable model system enables us to analyze nuclear architecture across several mutant backgrounds, and we are currently exploring methods to control expression of the Dam fusion proteins in a temporal manner and in specific tissues. (csic.es)
  • gene
  • The nuclear envelope (NE) has emerged as an important structure that serves numerous pivotal roles in the cell including compartmentalization, control of nuclear position and morphology, contribution to cell stability, chromatin organization and regulation of gene expression. (csic.es)
  • complexes
  • In the past 15 years, Linkers of the Nucleoskeleton to the Cytoskeleton (LINC complexes) have emerged as evolutionary-conserved molecular devices that span the nuclear envelope and provide interacting interfaces for cytoskeletal networks and molecular motors to the nuclear envelope. (nih.gov)
  • Here, we review the molecular composition of LINC complexes and focus on how their genetic alteration in vivo has provided a wealth of information related to the relevance of nuclear positioning during tissue development and homeostasis with a special emphasis on the central nervous system. (nih.gov)
  • There are many subtypes of ANAs such as anti-Ro antibodies, anti-La antibodies, anti-Sm antibodies, anti-nRNP antibodies, anti-Scl-70 antibodies, anti-dsDNA antibodies, anti-histone antibodies, antibodies to nuclear pore complexes, anti-centromere antibodies and anti-sp100 antibodies. (wikipedia.org)
  • These lamella have pore complexes which are identical to those of the nuclear cover. (wikipedia.org)
  • morphology
  • In routine clinical diagnosis of cancer, the procedure is very basic: nuclear morphology is used as a common assessment of the degree of malignancy, and hence acts as a prognostic and predictive indicator of the disease. (biomedcentral.com)
  • Furthermore, though the atypical nuclear morphology of cancer cells is believed to be a consequence of oncogenic signaling, the molecular basis remains unclear. (biomedcentral.com)
  • We used immunofluorescence microscopy to analyze nuclear morphology, flow cytometry to analyze cellular DNA content, and fluorescence in situ hybridization to examine cell ploidy of the lamin A/C-suppressed cells. (biomedcentral.com)
  • causes nuclear
  • We found that DMPK localizes to the NE and coimmunoprecipitates with Lamin-A/C. Overexpression of DMPK in HeLa cells or C2C12 myoblasts disrupts Lamin-A/C and Lamin-B1 localization and causes nuclear fragmentation. (uniprot.org)
  • fuse
  • In addition, we show that the vesicles bound under these conditions will fuse into an intact nuclear envelope when incubated with the soluble fraction of a Xenopus egg nuclear assembly extract. (rupress.org)