Nscs stem cell res. Medical search. Frequent questions

Moreover, there was also improved maintenance and increased neuronal differentiation of NSCs isolated from the SVZ of old Becn1 KI mice in in vitro assays. (biomed.news)
Until now, gene manifestation analyses performed in the V/SVZ-RMS-OB neurogenic program relied either on microdissection of cells4 or on cell sorting predicated on manifestation of a restricted group of membrane markers5,6 define particular differentiation stages. (bios-mep.info)
Even though the stem cell compartments from the lateral and dorsal lineages are bodily separated, these areas harbor progenitors at different differentiation phases (neural stem cells [NSCs], transit amplifying progenitors and migrating neuroblasts). (bios-mep.info)
More than the next times and hours, fairly homogeneous and timed cohorts of cells go through the various differentiation phases in the V/SVZ as well as the RMS to finally integrate as. (bios-mep.info)
These cells are the original precursors of various cells in a tumor, whereas other intratumoral cells are limited in terms of their potential for proliferation and pluripotent differentiation. (biomedcentral.com)
In addition, normal adult stem cells (ASCs) self-renew, proliferate infinitely, have a multidirectional differentiation potential, similar to that of tumor cells, and can transform more easily into tumor stem cells than non-stem cells [ 20 , 21 ]. (biomedcentral.com)
However, recent identification of spontaneous cell fusion and limited neuronal differentiation has tempered initial optimism. (ox.ac.uk)
In this review we summarized the recent progress in study of basic stem cell biology, on the prospective identification of NSCs, the elucidation of the mechanisms of ontogenic changes, potential differentiation, and their therapeutic applications. (kiev.ua)
Zozulya Yu.A., Lisyaniy NI, Tsymbalyuk VI, Semenova VM Neurogenic differentiation of stem cells. (kiev.ua)
Ghasemi-Mobarakeh L, Prabhakaran MP, Tian L, Shamirzaei-Jeshvaghani E, Dehghani L, Ramakrishna S. Structural properties of scaffolds: Crucial parameters towards stem cells differentiation. (wjgnet.com)
Scaffolds play an important role in tissue engineering as a substrate that can mimic the native extracellular matrix and the properties of scaffolds have been shown to affect the cell behavior such as the cell attachment, proliferation and differentiation. (wjgnet.com)
The Hirschi Lab is broadly interested in endothelial cell differentiation, proliferation, specification and function during development. (hirschilabuva.com)
The lab also uses human stem cell culture models to study the regulation of human endothelial cell differentiation and specification, and to study the functional roles of endothelial cells in regulating tissue-resident stem cells. (hirschilabuva.com)
Regulation of endothelial cell differentiation and specification. (hirschilabuva.com)
Serra M, Brito C and Alves PM. "Bioengineering strategies for stem cell expansion and differentiation. (sciepub.com)
Khadra M, Lyngstadaas SP, Haanaes HR, Mustafa K. "Effect of laser therapy on attachment, proliferation and differentiation of human osteoblast-like cells cultured on titanium implant material. (sciepub.com)
Co-transplantation of schwann cells promotes the survival and differentiation of neural stem cells transplanted into the injured spinal cord[J]. Dev Neurosci, 2005, 27(1):20-26. (sdu.edu.cn)
Moreover, pathological processes, such as neuroinflammation, stroke or epilepsy, are able to induce proliferation and differentiation of NSCs and NPCs. (jneurology.com)
The concept of the cancer stem cell is based on the fact that a subpopulation of glioma cells share common features with NSCs, including self-renewal and the differentiation potential to glial and neuronal cells [ 4 ]. (biomedcentral.com)
The lack of complete and high-quality epigenetic and transcriptome maps in GSCs hinders our understanding of the underlying critical differences at the transcriptional level between malignant GSCs and normal NSCs, as well as the epigenetic machinery involved in regulating their proliferation and differentiation. (biomedcentral.com)
Synaptamide is a potent mediator for neurogenic differentiation of NSCs acting through PKA/CREB activation. (adooq.com)

A neuronal lineage marker is an endogenous tag that is expressed in different cells along neurogenesis and differentiated cells such as neurons. (wikipedia.org)
Thus, it was possible to identify different types of neurons, as Golgi Cell, Golgi I and Golgi II. (wikipedia.org)
This novel approach aims to replace degenerated dopaminergic (DAergic) neurons with new ones or provide a new source of cells that can differentiate into DAergic neurons . (bvsalud.org)
The rational of neural stem cells (NSCs) in the treatment of neurological disease is either to replace dead neurons or to improve host neuronal survival the latter of which has got less attention and the underlying mechanism is as yet little known. (biobender.com)
generating specific cells to displace dropped neurons [2 3 continues to be challenged RASGRP2 since it is considered to become much less positive for the neurological disease where massive neuronal reduction occurs in huge parts of the mind [4]. (biobender.com)
Lately it is becoming known that grafted NSCs can handle stimulating endogenous fix systems and rescuing web host neurons [5-8]. (biobender.com)
Finally, adult-born neurons display a wide spectral range of morphologies, projection patterns, and focuses on.1 Lineage research demonstrated Apremilast small molecule kinase inhibitor how the diversity of OB interneurons Apremilast small molecule kinase inhibitor is closely linked with their spatial origin in the stem cell compartment. (bios-mep.info)
The finding that neuronal heterogeneity depends upon their site of source led to the idea how the stem cell market represents a mobile mosaic where populations of stem cells in described dorsoventral and anteroposterior positions are predetermined to create particular neurons for the OB.2 This, subsequently, means that molecular determinants, for instance, expressed TFs differentially, underlie early destiny standards and neuronal function and connectivity eventually. (bios-mep.info)
The idea to use transplants of dopa- ment of protocols that allow generation of fully functional mine-producing cells to substitute for the lost midbrain and safe midbrain dopamine neurons from stem cells. (lu.se)
VM), showed that the recovery of motor functions induced implanted either (1) as a solid piece in the lateral ven- by the grafted fetal dopamine neurons was well cor- tricle6 or a cortical cavity8 adjacent to the denervated related with the extent of graft-derived reinnervation caudate-putamen, or (2) as a crude cell suspension of the host caudate-putamen. (lu.se)
In the zebrafish, rprma mRNA is expressed in the olfactory placodes (OP) and olfactory epithelium (OE), rprmb is observed in the tectum opticum (TeO) and trigeminal ganglion (Tg), whereas rprml is found primarily in the telencephalon (Tel). At protein level, RPRM is present in a subset of cells in the OP, and neurons in the OE, TeO, hindbrain and sensory peripheral structures. (frontiersin.org)
Although electroporation and viral vector-mediated gene delivery techniques have been used to introduce exogenous DNA into chicken embryonic cells, transducing neurons efficiently and specifically remains challenging. (biomedcentral.com)
This is consistent with a negative-feedback loop, in which newly generated neurons modulate cell division of SVZ stem cells. (jneurosci.org)
However, when ectopically transplanted, neurosphere-derived cells from either region are largely unable to generate neurons. (silverchair.com)
With regard specifically to embryonic and adult cerebellar stem cells, we observe that they are able to give rise to neurons that resemble different select classes of cerebellar subclasses when grafted into the perinatal host cerebellum. (silverchair.com)
We demonstrate that neural stem/progenitor cell (NSPC) self-renewal and spatiotemporal generation of neurons and other cell types are severely impacted by the loss of Ythdf2 in embryonic neocortex. (biomedcentral.com)
In these regions, neural stem cells (NSCs) and neural progenitor cells (NPCs) have been reported to proliferate and produce postmitotic neurons. (jneurology.com)
In the last decade, cortical adult neurogenesis and its neural stem cells (NSCs), which are self-renewing and can generate neurons, astrocytes and oligodendrocytes, and neural progenitor cells (NPCs), which have a tendency to differentiate into certain types of neurons, have been found in the cerebral cortex of adult mammals 3 . (jneurology.com)
Moreover, certain drugs, such as antidepressants, can accelerate production of neurons from cortical NPCs, and increased neurons from cortical NPCs have the inhibitory effect on neuronal cell death, which is induced by global ischemia 4 . (jneurology.com)
Furthermore, in the last decade, several studies have found NSCs and NPCs capable of generating new neurons in mammals, including mice, rats, and humans. (jneurology.com)
These reports indicate that cortical NSCs and NPCs may produce neurons in a damage or stimulation-dependent manner. (jneurology.com)

Depending on the goal of a particular study, one can use neural stem cells markers, neural progenitor cell markers, neuron markers or PNS neuronal markers. (wikipedia.org)
Human induced pluripotent stem cell-derived neural stem/progenitor cell (hiPSC-NS/PC)-based cell transplantation has emerged as a groundbreaking method for replacing damaged neural cells and stimulating functional recovery, but its efficacy is strongly influenced by the state of the injured spinal microenvironment. (biomedcentral.com)

Induced pluripotent stem cells (iPSCs), mesenchymal stem cells (MSCs), neural stem cells (NSCs), and embryonic stem cells (ESCs) are among the cells considered for transplantation therapies . (bvsalud.org)

After pharmacologically-induced elimination of proliferative cells in the subventricular zone (SVZ), there was enhanced re-activation of quiescent NSCs in old Becn1 KI mice as compared to those in WT mice, with more efficient exit from quiescent status to generate proliferative cells and neuroblasts. (biomed.news)
In the OB, large numbers of interneurons are permanently issued from stem cells residing in the ventricular/subventricular zone (V/SVZ) lining the forebrain ventricles. (bios-mep.info)
Altogether our FACS (fluorescence-activated cell sorter) analyses reveal that the neonatal subventricular zone is far more heterogeneous than previously suspected and our studies provide new insights into the signals and mechanisms that regulate their self-renewal and proliferation. (karger.com)
In the adult mammalian subventricular zone (SVZ), GFAP-positive neural stem cells (NSCs) generate neuroblasts that migrate tangentially along the rostral migratory stream (RMS) toward the olfactory bulb (OB). (jneurosci.org)

The transplantation of bone marrow-derived mesenchymal stem cells (BMMSCs) alleviates neuropathology and improves cognitive deficits in animal models with Alzheimer's disease. (biomedcentral.com)
In recent years, it has been an active research area to replace neural tissue loss through transplantation of neural stem cells (NSCs) for the treatment of AD [ 3 ]. (biomedcentral.com)
In this regard, the growth and proliferation of the neural stem cells in vitro are critical as the number of cells available for transplantation is essential to achieve beneficial effect in replacing injury or loss of the neuronal cells. (biomedcentral.com)
Thus, the introduction or mobilization of NSCs could be expected to lead to CNS regeneration by recapitulating normal CNS development, in terms of the activation of the endogenous regenerative capacity and cell transplantation therapy. (kiev.ua)
Schwann cells (SCs) have a wide range of applications as seed cells in the treatment of nerve injury during transplantation. (ac.ir)
Transplantation of a multipotent cell population from human adipose tissue induces dystrophin expression in the immunocompetent mdx mouse[J]. J Exp Med, 2005, 201(9):1397-1405. (sdu.edu.cn)
Co-transplantation of neural stem cells and Schwann cells within poly (L-lactic-co-glycolic acid) scaffolds facilitates axonal regeneration in hemisected rat spinal cord[J]. Chin Med J, 2013, 126(5):909-917. (sdu.edu.cn)
Co-transplantation of neural stem cells and NT-3-overexpressing Schwann cells in transected spinal cord[J]. J Neurotrauma, 2007, 24(12):1863-1877. (sdu.edu.cn)
Comparing the potential of neural stem cells derived from the forebrain and cerebellum, we find that progeny derived from each of these brain regions retain regional character in vitro as well as after homotopic transplantation. (silverchair.com)
Most notably, upon transplantation to the perinatal cerebellum, cerebellar stem cells from all ages are able to acquire the position and mature electrophysiological properties of cerebellar granule cells. (silverchair.com)
Multiple research groups have developed artificial mitochondrial transfer/transplantation (AMT/T) methods that transfer healthy mitochondria into damaged cells and recover cellular function. (biomedcentral.com)
Following cell transplantation into a favorable environment, the transplanted NS/PCs survived well, facilitating remyelination and neuronal regeneration in host tissues. (biomedcentral.com)

First, a 'gliogenic switch' enables NSCs to progress from neurogenesis to the generation of astrocytes and oligodendrocytes. (nature.com)
We found that increased autophagy protected NSCs from exhaustion and promoted neurogenesis in old (≥18-months-old) mice compared with age-matched wild-type (WT) mice, although it did not affect NSCs in young (3-months-old) mice. (biomed.news)
Recently, adult neurogenesis in the cerebral cortex is becoming clear gradually, and cortical NSCs and NPCs are identified in a few mammals. (jneurology.com)
Historically, the discovery of cortical adult neurogenesis is old, and identifying generation of new cells in the adult cerebral cortex was first reported in 1912 5 . (jneurology.com)
Neurogenesis generates various functional neural cell types from multipotent neural stem cells (NSCs) in the mammalian CNS, which participate in specific neural activities and responses [ 3 ]. (biomedcentral.com)

Curr Stem Cell Res Ther;2023 Feb 22. (bvsalud.org)
Cancer Res (2023) 83 (7_Supplement): 6028. (aacrjournals.org)
Res Sq. 2023. (stembook.org)
Stem Cell Rev Rep. 2023. (stembook.org)
Lin A, Gallicchio VS. (2023) Stem Cell Therapy for Glioblastoma. (genesispub.org)

Astrocytes are the most abundant glial cells in the mammalian central nervous system and they serve essential functions in brain development and homoeostasis. (nature.com)
Astrocytes comprise the most abundant glial cells in the mammalian brain. (biomedcentral.com)
In addition to intracellular mitochondrial movement, intercellular mitochondrial transfer between mammalian cells has recently been discovered. (biomedcentral.com)

The macroautophagy/autophagy deficiency in wdr45 wdr45b DKO cells is ameliorated by suppression of O-GlcNAcylation, which promotes autophagosome maturation. (biomed.news)
Critically, we show that sequential treatment with the mitogens epidermal growth factor (EGF) and fibroblast growth factor-2 (FGF-2) followed by postnatal hippocampal astrocyte conditioned medium significantly promotes the generation of neurofilament(+)/beta-tubulin(+) cells from bone marrow precursors. (ox.ac.uk)
However, whether RESV promotes function of senescent stem cells in alleviating age-related phenotypes remains largely unknown. (thno.org)
The present study showed that icariin promotes the growth and proliferation of neural stem cells from rat hippocampus in a dose-dependent manner. (biomedcentral.com)
Retinoic Acid Promotes Endothelial Cell Cycle Early G1 State to Enable Human Hemogenic Endothelial Cell Specification. (hirschilabuva.com)
Time-lapse videography of living cells captures spectacular intracellular mitochondrial movement, and this movement promotes mitochondrial connections to form a dynamic mitochondrial network [ 3 ]. (biomedcentral.com)

After cells are isolated from tissue or differentiated from pluripotent precursors, the resulting population needs to be characterized to confirm whether the target population has been obtained. (wikipedia.org)
Errors arising in stem and progenitor cells have a much greater influence on the tissue in which they are found than errors arising in postmitotic differentiated cells. (biomedcentral.com)
Ethical and practical considerations limit the availability of human fetal-derived neural tissue and highlight the need to consider alternative sources of human NSCs. (ox.ac.uk)
However, the growing interest in the development of innovative treatments that are aimed at the regeneration damaged tissue CNS is based on the latest research in the field of stem cells and neurology. (kiev.ua)
The levels of catalase (CAT) and glutathione (GSH) were measured in PC12 cells and Drosophila brain tissue. (sdbonline.org)
GSOs also mitigated the deleterious effects of GLU on the mitochondrial membrane potential and Cyt C release, thus alleviating mitochondrial dysfunction, and increased GSH levels and CAT activity in both cells and Drosophila brain tissue. (sdbonline.org)
Stem cells have attracted much interest in tissue engineering as a cell source due to their ability to proliferate in an undifferentiated state for prolonged time and capability of differentiating to different cell types after induction. (wjgnet.com)
These early trials are showing roles for stem cells both in replacing damaged tissue as well as in providing extracellular factors that can promote endogenous cellular salvage and replenishment. (biomedcentral.com)
The use of patient's own bone marrow aspirates, hematopoietic stem cells and MSCs, for heart muscle tissue repair can be puzzling because these cells do not normally contribute to the cardiac lineage types that are desired. (biomedcentral.com)
Here, we present a multimodal -omics platform for the comprehensive study of cell populations in complex tissue, which has allowed us to characterize the cells involved in wound healing across both time and space. (stanford.edu)
The latter findings point to the participation of Nogo-A/NgR1 signaling in the regulation of other aspects of growth, such as tissue expansion or turnover by cell proliferation. (jneurosci.org)
Tissue-engineering and cell-based therapies, two prominent branches of Regenerative Medicine, are considered as the most suitable techniques to repair the tissue damage. (sciepub.com)
9] Alessandri G, Emanueli C, Madeddu P. Genetically engineered stem cell therapy for tissue regeneration[J]. Ann N Y Acad Sci, 2004, 1015:271-284. (sdu.edu.cn)
In addi tion, LNCaP C4 2B cells express a promiscuous kind in the androgen receptor, possessing the most AR frequent sub stitution, which can be repeatedly observed in prostate cancer selleck chemical tissue specimens of individuals with AIPC. (hdac-inhibitors.com)
The acute-phase HGF-treated group exhibited vascularization, diverse anti-inflammatory effects, and activation of endogenous neural stem cells after SCI, which collectively contributed to tissue preservation. (biomedcentral.com)

NSCs can be cultured in vitro as neurospheres that are composed of neural stems cells and progenitors. (biomedcentral.com)
It is essential to have sensitive and reproducible methods of either quantifying or isolating these stem cells and progenitors to understand their intrinsic properties and how extrinsic signals regulate their development. (karger.com)
However, stem cells are difficult to distinguish from multipotential progenitors, which may look and act like them. (karger.com)
Here we define a 4-color flow cytometry panel using CD133, LeX, CD140a, NG2 to define a neural stem cell (NSC) as well as 4 classes of multipotential progenitors and 3 classes of bipotential progenitors, several of which have not been described previously. (karger.com)

Investigating astrocyte maturation in a cell culture model revealed that in vitro-differentiated astrocytes lack expression of many mature astrocyte-specific genes, including genes for the transcription factors Rorb, Dbx2, Lhx2 and Fezf2. (nature.com)
The NSCs can grow and multiply in vitro medium, and then be activated to differentiate into different nerve cells to be transplanted within the brain to replace damaged cells. (biomedcentral.com)
Genetic ablation of TMEM9 inhibits colorectal cancer cell proliferation in vitro, ex vivo and in vivo mouse models. (nature.com)
Using biologically relevant elastic substrates to study cell function in vitro has proven beneficial, as the in vivo microenvironment usually is much softer than rigid plastic dishes. (5dok.org)
Transgene expression by two neuron-specific promoters (cCaMKII and cNestin) was preliminarily verified in vitro in cultured brain cells, and in vivo , expression levels of an EGFP transgene in brain cells by neuron-specific promoters were comparable to or higher than those of the ubiquitous CMV promoter. (biomedcentral.com)
Since only small numbers of cells are obtainable from the patient, it requires expansion of cells in vitro . (sciepub.com)
Nevertheless, these primary cells grow slowly in vitro and lose their general characteristics during prolonged culturing period. (sciepub.com)
The cytocompatibility of PLGA scaffolds coated with different concentration of chitosan with schwann cells in vitro[J]. Chin J Aesth Med, 2009, 18(11):1627- 1631. (sdu.edu.cn)
Furthermore, many research possess also offered proof that SiO2NP trigger irregular groupings of topoisomerase I in the nucleoplasm of cells, and pro-inflammatory activation both in vivo and in vitro [26-29]. (irjs.info)
30] exhibited in an in vitro research that amorphous SiO2NP (15 and 46 nm) considerably decreased the viability of individual alveolar epithelial cells A549 in a dosage- and period- reliant way. (irjs.info)

Further, the mechanism of ICA-induced cell proliferation of neural stem cells was investigated by analyzing the gene and protein expression of cell cycle related genes cyclin D1 and p21. (biomedcentral.com)
Exploring the effect of pharmacological manipulation, such as usage of traditional medicine, on the growth and proliferation of neural stem cells is thus valuable towards the development of the stem cells therapies of the neurodegenerative disorders. (biomedcentral.com)

Hematopoietic stem and progenitor cells (HSPCs) that are essential for establishment and maintenance of the adult blood system are generated during embryonic definitive hematopoiesis. (hirschilabuva.com)
Research in the Hirschi Lab aims to uncover the developmental mechanisms of hemogenic endothelial cell specification and endothelial-to-hematopoietic transition, and apply insights gained to the generation of human HSPCs for clinical therapies. (hirschilabuva.com)

Cell Res Ther. (kiev.ua)

Dibutyryl-cAMP is a cell-permeable PKA activator by mimicing the action of endogenous cAMP. (adooq.com)

Recently disease -modifying strategies like cell replacement therapies combined with other therapeutic approaches , such as utilizing natural compounds or biomaterials , are proposed to modify the underlying neurodegeneration. (bvsalud.org)
Allogenic Umbilical Cord-Derived Mesenchymal Stromal Cells Sustain Long-Term Therapeutic Efficacy Compared With Low-Dose Interleukin-2 in Systemic Lupus Erythematosu. (stembook.org)
4. Gross G, Eshhar Z. Therapeutic potential of T-cell chimeric antigen receptors (CARs) in cancer treatment: counteracting off-tumor toxicities for safe CAR T-cell therapy. (ac.ir)
Although the goal of Glioblastoma is not only to treat the disease but to cure it, current animal in vivo studies have shown promising therapeutic effects in mesenchymal stem cells, induced neural stem cells, and induced pluripotent stem cells. (genesispub.org)
While research has shown that each stem cell poses its own risk to the disease, their therapeutic effects have also shown encouraging and promising results. (genesispub.org)
This review outlines the research, progress, and potential therapeutic effects different stem cells have on Glioblastoma. (genesispub.org)
This study aimed to review the current progress researchers have made regarding stem cell therapeutic effects for Glioblastoma, how it is applied, and to discover the potential future progress and treatments these stem cells can create. (genesispub.org)
A new study reveals comprehensive and unique epigenetic properties of glioma stem cells, leading to novel molecular insights and therapeutic potentials toward glioblastoma multiforme treatment. (biomedcentral.com)

We used conjugated polymers to develop a novel neural stem cell culture substrate with anchored growth factors to promote cell self-renewal. (5dok.org)
These stem cells have self-renewal capabilities, and stem cells, like the mesenchymal stem cells, can differentiate into various lineages from the mesoderm. (genesispub.org)

demonstrated that c Nf2 overexpression in lens epithelial cells inhibits cell proliferation [ 9 ]. (biomedcentral.com)

According to the hypothesis, only a small group of stem cell-like tumor cells can form new tumors. (biomedcentral.com)
Cell invasion is a crucial step of tumor metastasis , finding new regulators of which offers potential drug targets for cancer therapy. (sdbonline.org)
Reactive astrocytes have been shown to enhance tumor cell proliferation, survival, invasive capabilities, and resistance to chemotherapy [ 7 ]. (biomedcentral.com)

Senescence of mesenchymal stem cells (MSCs) and the related functional decline of osteogenesis have emerged as the critical pathogenesis of osteoporosis in aging. (thno.org)
There are many studies involving autologous therapies and some allogenic therapies, based on the recovery of mobilized bone marrow cells, including mesenchymal stem cells (MSCs) and adipose derived stem cells that also include the stromal or adherent cell type that has an MSC phenotype. (biomedcentral.com)
This includes bone and cartilage repair, cell types into which MSCs readily differentiate, and immune conditions such as graft versus host disease and autoimmune conditions that utilize the MSC's immune suppressive properties. (biomedcentral.com)

11. Park HW, Lim MJ, Jung H, Lee SP, Paik KS, Chang MS. Human mesenchymal stem cell‐derived Schwann cell‐like cells exhibit neurotrophic effects, via distinct growth factor production, in a model of spinal cord injury. (ac.ir)

Biochem Biophys Res Commun. (biomed.news)

Cancer Cell Int. 2022;22(1):330. (stembook.org)

Stem cells rely on extracellular signals produced by the niche, which dictate their ability to self-renew, expand and differentiate. (karger.com)

The discovery of various proteins specific to certain cells led to the production of cell-type-specific antibodies that have been used to identify cells. (wikipedia.org)
Furthermore, GSOs protected cells against GLU-induced apoptosis by reducing the expression of the mitochondrial apoptosis-associated Bcl-2 family effector proteins and protected cells from GLU-induced oxidative damage by increasing the nuclear translocation of Nrf2 and HO-1 expression. (sdbonline.org)
Exosomes contain various molecular constituents of their cell of origin, including proteins and RNA. (exosome-rna.com)
After immunofluorescence identification, we identified differentially expressed proteins in the ASCs and NSCs using isobaric tags for relative and absolute quantitation (iTRAQ) combined with high-resolution Orbitrap liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). In addition, all the differentially expressed proteins were analyzed by Gene ontology (GO) analysis and Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis. (ac.ir)
A total of 122 differentially expressed proteins in ASCs and NSCs were screened. (ac.ir)

CNS-resident glial progenitor/stem cells produce Schwann cells as well as oligodendrocytes during repair of CNS demyelination. (ac.ir)
Gliomas often originate from three types of glial cells: oligodendrocytes, ependymal cells, and astrocytes - however, astrocytic gliomas hold 70% of all glioma origins [4]. (genesispub.org)

We performed gain and loss of function studies for leukemia inhibitory factor (LIF) and showed a depletion of NSCs, a subset of multipotential neural precursors and immature oligodendrocytes in LIF null mice. (karger.com)

Like the CTCs, LNCaP C4 2B cells also express PTCH, EGFR and ErbB2 RNA. (hdac-inhibitors.com)
The development of LNCaP C4 2B cells in androgen no cost medium was appreciably reduced by therapy using the Hedgehog pathway inhibi tor cyclopamine, the EGFR inhibitor gefitinib and also the EGFR and ErbB2 inhibitor lapatinib. (hdac-inhibitors.com)
The ErbB inhibitors gefitinib and lapat inib also inhibited EGF induced autophophor ylation of your EGFR in LNCaP C4 2B cells. (hdac-inhibitors.com)

Endothelial Cell Development and Its Application to Regenerative Medicine. (hirschilabuva.com)
7] Gardner R L. Stem cells and regenerative medicine: principles, prospects and problems[J]. C R Biol, 2007, 330(6-7):465-473. (sdu.edu.cn)

The stage-specific embryonic antigen 4 (SSEA4) is com- isolate the NSCs from neonatal mice and rats (Campos monly used as a cell surface marker to identify the pluri- et al. (lu.se)
Immunohisto- have been used for positive selection of NSCs from em- chemistry on human embryonic central nervous system bryonic mice (Nagato et al. (lu.se)
Principal microglia cells civilizations Principal microglia cells had been isolated from 2-day-old ICR mice as defined previously [29]. (biobender.com)
In this study, we used Becn1F121A/F121A knockin mice (designated as Becn1 KI mice) with the F121A allele in the autophagy gene Becn1 to investigate the consequences of enhanced autophagy in postnatal neural stem cells (NSCs) during aging. (biomed.news)
Mesenchymal stem cells facilitate axon sorting, myelination, and functional recovery in paralyzed mice deficient in Schwann cell‐derived laminin. (ac.ir)
These cells were initially isolated and characterised following growth in castrated athymic mice of androgen http://www.selleckchem.com/products/arq-197.html dependent LNCaP prostate cancer cells from your web site of bony metastasis. (hdac-inhibitors.com)

Astrocytes are generated from neural stem cells (NSCs) during late embryonic and early postnatal stages. (nature.com)
During a subsequent phase of maturation in the first few postnatal weeks, immature astrocytes exit the cell cycle and aquire a fully mature phenotype 1 . (nature.com)
Postnatal astrocytes promote neural induction from adult human bone marrow-derived stem cells. (ox.ac.uk)

Tsymbalyuk V.I., Medvedev V.V. Neurogenic stem cells. (kiev.ua)

Activated Schwann cells (ASCs) and normal Schwann cells (NSCs) were obtained from adult Wistar rat sciatic nerves. (ac.ir)

We demonstrated that exogenous DNA can be effectively introduced into neuronal cells in living embryos by in ovo electroporation with constructs containing neuron-specific promoters. (biomedcentral.com)
Promoters are a key factor determining the expression dynamics of transgenes, and various promoters are being studied in neuronal cells. (biomedcentral.com)

take on this vital challenge to generate comprehensive genome-wide epigenomic profiling for various forms of cytosine modifications of DNA at single-base resolution and signature histone modifications of enhancers and transcriptomes of GSCs isolated from patient-derived xenografts, with NSCs isolated from fetal brains as controls [ 6 ]. (biomedcentral.com)

Skeletal muscle growth and regeneration involves the activity of resident adult stem cells, namely satellite cells (SC). (sdbonline.org)
Dental and orofacial mesenchymal stem cells in craniofacial regeneration: the prosthodontist's point of view. (ac.ir)
5] Schmalenberg K E, Uhrich K E. Micropatterned polymer substrates control alignment of proliferating Schwann cells to direct neuronal regeneration[J]. Biomaterials, 2005, 26(12):1423-1430. (sdu.edu.cn)

More research teams are accelerating the use of other types of adult stem cells, in particular neural stem cells for diseases where beneficial outcome could result from either in-lineage cell replacement or extracellular factors. (biomedcentral.com)

cAMP and Schwann cells promote axonal growth and functional recovery after spinal cord injury. (ac.ir)

Stem Cell Therapy for the Treatment of Parkinson's Disease: What Promise Does it Hold? (bvsalud.org)
With this in mind, cell replacement therapy is a new strategy for treating PD. (bvsalud.org)
In the present review , we discuss the current advances in cell replacement therapy for PD and summarize the existing experimental and clinical evidence supporting this approach. (bvsalud.org)
Importantly, at late stages of tumorigenesis cancer cells employ autophagy to sustain proliferation in unfavorable conditions, including anti-cancer drug therapy. (biomed.news)
Targeted Therapy for Peri-prosthetic Osteolysis Using Macrophage Membrane-encapsulated Human Urine-derived Stem Cell Extracellular Vesicles. (stembook.org)
Low c-Kit expression identifies primitive therapy-resistant CML stem cells. (stembook.org)
Microenvironmental CXCL12 deletion enhances Flt3-ITD acute myeloid leukemia stem cell response to therapy by reducing p38 MAPK signaling. (stembook.org)
Cell Therapy for Parkinsons Disease: What Next? (lu.se)
3. Huang H, Mao G, Chen L, Liu A. Progress and challenges with clinical cell therapy in neurorestoratology. (ac.ir)
Cell therapy for multiple sclerosis. (ac.ir)
14. Lavdas AA, Papastefanaki F, Thomaidou D, Matsas R. Cell adhesion molecules in gene and cell therapy approaches for nervous system repair. (ac.ir)
Evaluation of low-level laser therapy of osteoblastic cells. (sciepub.com)
Gene expression The affect of DADS therapy on cytokine induced metalloproteinase gene expression was assessed from the SW1353 cell line by TaqMan qRT PCR. (hdac-inhibitors.com)

2005). Notch1 and syndecan-1 potent human embryonic stem (ES) cells. (lu.se)
Surprisingly, the related cytokine, CNTF, was less potent than LIF in increasing the NSCs and more potent than LIF in increasing the PDGF responsive multipotential precursors. (karger.com)
H 89 dihydrochloride is a cell-permeable, selective, reversible, ATP-competitive and potent inhibitor of protein kinase. (adooq.com)

Primary neural stem cells were prepared from the hippocampus of newly born SD rats, and cells were cultured in special stem cell culture medium. (biomedcentral.com)
Thereafter, using a combination of autoradiography and electron microscopy, Kaplan demonstrated that new cells containing tritiated thymidine are stellate cells that have an axonal hillock, initial segment, and synapses on their dendrites and cell bodies in the cerebral cortex of adult rats 7 . (jneurology.com)

1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)

In the present study we were prompted to study whether NSCs regulate resident microglial activity TLR or ERK1/2 signal and if this is true whether this is responsible for the improved host neuronal viability. (biobender.com)
dGLYAT was found to regulate Gadd45 -mediated JNK pathway activation and cell invasion. (sdbonline.org)
These results indicate that the two intestinal secretory peptides antagonistically regulate adult lifespan and intestinal senescence through multiple pathways, irrespective of insulin, which implicates a complementary gradient distribution of each of the hormone-producing EEs, consistent with local requirements for cell activity along the posterior midgut. (bioone.org)

We demonstrate that neural stem cells exist within the embryonic and adult cerebellum. (silverchair.com)

In this study, we demonstrate the monoclonal neural and mesodermal potential of adult human bone marrow mesenchymal cells. (ox.ac.uk)
While numerous teams continue to refine and expand the role of bone marrow and cord blood stem cells for their vanguard uses in blood and immune disorders, many others are looking to expand the uses of the various types of stem cells found in bone marrow and cord blood, in particular mesenchymal stem cells, to uses beyond those that could be corrected by replacing cells in their own lineage. (biomedcentral.com)
A significant proportion of clinical studies that are underway involve bone marrow and cord blood stem cells for blood and immune disorders [ 3 ] and cancers. (biomedcentral.com)
Characterization of human bone marrow fibroblast colony-forming cells (CFU-F) and their progeny. (sciepub.com)

At the same time, the first three trials using cells derived from pluripotent cells have begun. (biomedcentral.com)

Additional treatment with TLR9 ligand CpG-ODN TLR9 inhibitor chloroquine (CQ) or ERK1/2 inhibitor U0126 confirmed that TLR9-ERK1/2 pathway was mixed up in NSCs-induced microglial activation. (biobender.com)
Collectively this research indicated that NSCs improve web host neuronal success by switching microglia from a negative to a neuroprotective phenotype in adult mouse human brain as well as the microglial TLR9-ERK1/2 pathway appears to take part in this NSCs-mediated recovery action. (biobender.com)
Macroautophagy/autophagy is emerging as a major pathway that regulates both aging and stem cell function. (biomed.news)
Firstly, loss of dGLYAT suppressed scrib depletion- or Egr overexpression-induced JNK pathway activation and invasive cell migration. (sdbonline.org)
This signaling pathway is a highly conserved cell-to-cell communication system that regulates cell fate in development and disease. (5dok.org)
We propose an opportunity to target the cancer cell/microenvironment interface instead of the Notch pathway itself in the development of cancer therapies. (5dok.org)
Eph/ephrin signaling is a cell-to-cell communication pathway, which regulates cell migration and proliferation. (5dok.org)

In the adult and perinatal forebrain, regionalized neural stem cells lining the ventricular walls produce different types of olfactory bulb interneurons. (bios-mep.info)

Cell Death Discov. (stembook.org)
Cell Death Discov 8(1): 459. (sdbonline.org)

Vascular perfusion is required for all tissues in the body to provide nutrients and oxygen, remove waste products, maintain fluid homeostasis, and traffic immune cells. (hirschilabuva.com)

On the cover: Human induced pluripotent stem cell-derived neuroepithelial-like stem cells stained for actin (green) and DNA (blue). (5dok.org)

Previous function proven that Apremilast small molecule kinase inhibitor in vivo mind electroporation may be used to transfect DNA-based vectors,7 or messenger RNA actually,8 in to the different stem cell compartments encircling the ventricle. (bios-mep.info)
Hall marks on the majority of prostate cancers in vivo and traits not shared with other established pros tate cancer cell lines which include PC3 and DU145. (hdac-inhibitors.com)

Using this technique, it is possible to locate gene expression to specific cell types in specific regions and observe how changes in this distribution occur throughout the development and correlate with the behavioral manipulations. (wikipedia.org)
Gene Manifestation in Space and Period Tiveron et al.3 attempt to systematically identify and characterize such destiny determinants predicated on high-resolution gene expression analyses functionally. (bios-mep.info)
This study evidently demonstrates the potentiating effect of ICA on neural stem cell growth and proliferation, which might be mediated through regulation of cell cycle gene and protein expression promoting cell cycle progression. (biomedcentral.com)
In the present study, we performed a comparative study of the ubiquitous CMV promoter and three neuron-specific promoters, chicken Ca2+/calmodulin-dependent kinase (cCaMKII), chicken Nestin (cNestin), and human synapsin I. We explored the possibility of manipulating gene expression in chicken embryonic brain cells using in ovo electroporation with the selected promoters. (biomedcentral.com)
Overexpression of the FOXP2 gene driven by the cNestin promoter in brain cells significantly affected expression levels of target genes, CNTNAP2 and ELAVL4 . (biomedcentral.com)
Through integrated analysis of single cell chromatin landscapes and gene expression states, coupled with spatial transcriptomic profiling, we are able to impute fibroblast epigenomes with temporospatial resolution. (stanford.edu)

Neural stem cells (NSCs) have generated considerable interest because of their potential as a source of defined cells for drug screening or cell-based therapies for neurodegenerative diseases. (ox.ac.uk)
There has been a rapid surge in clinical trials involving stem cell therapies over the last two to three years and those trials are establishing the clinical pathways for an emergent new medicine. (biomedcentral.com)

ES) cells are known to be sustained during CNS devel- enriched in neurosphere-initiating cel s. (lu.se)
2004), the ABC trans- panded neurosphere cells and also mark subfractions porter Bcrp1/ABCG2 (Hulspas et al. (lu.se)
of neurosphere-initiating cells. (lu.se)

The classification of each glioma is based on its cell origin and molecular characteristics, which includes acquired mutations [4]. (genesispub.org)

Anatomy63

Organisms16

Diseases13

Chemicals and Drugs59

Analytical, Diagnostic and Therapeutic Techniques and Equipment52

Phenomena and Processes52

Disciplines and Occupations4

Technology, Industry, Agriculture2

Information Science2

Named Groups1

Health Care1

Differentiation21

Neurons21

Progenitor cell2

Embryonic stem1

Subventricular zone4

Transplantation12

Neurogenesis5

20235

Mammalian3

Promotes6

Tissue15

Progenitors4

Vitro10

Proliferation of neural stem2

Hematopoietic2

Ther1

Endogenous1

Therapeutic8

Self-renewal2

Epithelial cells1

Tumor3

MSCs3

Mesenchymal stem1

Biochem Biophys Res Commun1

20221

Differentiate1

Proteins5

Glial2

Subset1

EGFR3

Regenerative Medicine2

Mice6

Postnatal3

Neurogenic1

ASCs1

Neuronal cells2

Generate1

Regeneration3

Adult stem1

Spinal1

Therapy13

Potent3

Rats2

Fetal1

Regulate3

Demonstrate that neural1

Bone marrow4

Pluripotent cells1

Pathway7

Forebrain1

Death Discov2

Homeostasis1

Induced pluripotent1

Vivo2

Gene6

Therapies2

Neurosphere3

Glioma1