• Promoter analyses revealed increased hypoxia-inducible factor-2 binding to the CXCL12 promoter under hypoxic conditions. (haematologica.org)
  • Pretreatment of murine myoblast (C2C12) cells with octyl-D-carnosine or carnosine enhanced HIF-1α protein expression, VEGF mRNA levels and VEGF release under hypoxic conditions. (frontiersin.org)
  • We applied a range of molecular and cellular techniques to measure cell survival, cell cycle progression, protein expression and DNA damage combined with a transcriptomic micro-array approach in D283-MED cells, for global gene expression analysis in acute and chronic hypoxic conditions. (biomedcentral.com)
  • Whilst U87MG shared the same dampened p53 activity, upon chemotherapeutic drug treatment in chronic hypoxic conditions, these cells used a different mechanism, independent of the DNA damage pathway. (biomedcentral.com)
  • Conversely, we observed no difference in expression of the homing CXCR4 receptor between cells cultured under normoxic and hypoxic conditions, indicating that hypoxia increased the absolute numbers of LTR-HSCs but not their homing potential after transplantation. (confex.com)
  • To clarify how hypoxia improved Notch-mediated expansion of LTR-HSCs, we performed scRNA-seq of CD34+ cells treated with Delta1 under normoxic or hypoxic conditions. (confex.com)
  • GBM stem and differentiated cells were incubated under hypoxic conditions and analyzed for pro-inflammatory gene expression and for invasive/migratory behavior. (biomedcentral.com)
  • Under hypoxic conditions activates the transcription of over 40 genes, including, erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. (abcam.cn)
  • Cancer Spheroids were successfully cultured Cells in Happy Cell ASM® in both normoxic and hypoxic conditions. (valelifesciences.com)
  • Previous studies have identified apoptosis as a significant mechanism underlying cell death during I/R injury in cultured cardiac myocytes ( 6 , 7 ), and that the inhibition of this apoptosis is able to prevent I/R injury ( 8 ). (spandidos-publications.com)
  • Furthermore, administration of EGCG in vitro was observed to prevent apoptosis of cardiomyocytes by regulating pro-apoptotic and anti-apoptotic proteins, including B cell lymphoma-2 (Bcl-2) and Bcl-2-associated X protein, and by simultaneously regulating caspase-3 in isolated rat hearts ( 13 ). (spandidos-publications.com)
  • Our study suggests that TFs can decrease cell apoptosis, which may be mediated by altering the expression of miR-21, PTEN/Akt, and Bcl/Bax. (hindawi.com)
  • In murine cardiomyocytes miR-21 was found to protect from hypoxia/reoxygenation (H/R)-induced cell apoptosis via regulation of its target gene PDCD4 [ 13 ]. (hindawi.com)
  • In this study, we investigate the influence of AST against glutamate- and hypoxia-induced apoptosis, as well as oxidative stress-induced necrosis, in primary rat RGC cultures. (molvis.org)
  • PI3K deactivates BAD via Akt-mediated phosphorylation and thus may inhibit hypoxia-induced apoptosis in neurons. (jneurosci.org)
  • MicroRNA-543 promotes cell invasion and impedes apoptosis in pituitary adenoma via activating the Wnt/β-catenin pathway by negative regulation of Smad7. (cancerindex.org)
  • HP75 cells treated with overexpressed miR-543 exhibited increased cell proliferation, migration and invasion, while decreased cell apoptosis as well as expression of Cleaved caspase-3 and Cleaved caspase-8 were observed. (cancerindex.org)
  • Hypoxia lessened the cellular stress response in both progenitors and HSCs, but the mitigation was more apparent in the HSC population (Fig. C, grey plots), and decreased apoptosis was observed only within the HSC-enriched cluster 5 (Fig. D). These findings are consistent with several reports indicating that HSCs are more vulnerable to strong ER stress than downstream progenitors due to their lower protein folding capacity. (confex.com)
  • Myocardial I/R injury may induce cell apoptosis and autophagy by activating oxidative stress and upregulating inflammatory mediators, ultimately resulting in irreversible fibrotic damage ( 3 ). (spandidos-publications.com)
  • MicroRNAs (miRNAs) are small endogenous RNAs that are associated with tumourigenesis, cell proliferation and apoptosis ( 4 , 5 ). (spandidos-publications.com)
  • Studies have shown that endothelial cells (ECs) are the direct targets of hypoxia and are involved in the pathogenesis process of HPH leading to cell hyperproliferation, inhibition of apoptosis and plexiform intima injuries (Ranchoux et al. (biomedcentral.com)
  • Increasing tumor size requires that GBM tumor cells maintain a balance between adaptation to hypoxia and cell death (apoptosis and central necrosis) through activation of hypoxia-inducible transcription factor 1 (HIF-1). (biomedcentral.com)
  • Bcl2/adenovirus EIB 19kD-interacting protein 3 (BNIP3) is a cell death factor that is a member of the Bcl-2 proapoptotic family recently shown to induce necrosis rather than apoptosis. (ox.ac.uk)
  • Hypoxia is a typical condition in solid tumors, and cancer-derived EVs support tumor growth and invasion of tissues by tumor cells. (timegate.com)
  • Researchers have understood for numerous years that the hypoxia found in tumors leads to more aggressive and harder to treat disease and ultimately, poor patient outcome. (mdpi.com)
  • Epigenetic mechanisms play important roles in the regulation of tumorigenesis, and hypoxia-induced epigenetic changes may be key drivers of the adaptation of cancer cells to the hypoxic microenvironment typical of solid tumors. (nih.gov)
  • Vascular endothelial growth factor (VE6E), which is under the control of hypoxia inducible factor-1 alpha (HIF-1 alpha), stimulates the proliferation of endothelial cells and increases cell permeability, promoting the growth, spread and metastasis of tumors. (fapesp.br)
  • Comparison from the metabolomic information between A549 and A549shHTATIP2 tumors proven that the lack of HTATIP2 manifestation resulted in improved tumor metabolic plasticity that allowed tumor cells to exploit substitute metabolic pathways for success and proliferation instead of counting on glutamine and essential fatty acids like a carbon resource to replenish TCA routine intermediates. (estme.org)
  • Our data recommend a mechanism where the absent HTATIP2 manifestation modulates tumor version to hypoxia and promotes an intense tumor phenotype by improving the HIF2-controlled -catenin/c-Myc/MCL-1 signaling, raising the susceptibility of tumors to sorafenib treatment-activated EMT procedure, and enhancing tumor metabolic plasticity. (estme.org)
  • We have shown recently that in breast and prostate tumors, as well as in breast tumor cell lines, HIF-1α has a crucial role in regulating, either directly or indirectly, the expression of pro-inflammatory genes. (biomedcentral.com)
  • Hypoxia is a common hallmark of solid tumors because of the Warburg effect. (biomedcentral.com)
  • A previous study showed that oxygen-sensitive multichain CAR-T cells are responsive to hypoxia [ 7 ], while a simple single-chain hypoxia-inducible CAR-T cells (HiCAR-T cells) could respond to hypoxia within solid tumors is elusive. (biomedcentral.com)
  • HIF-1-dependent regulation of hypoxic induction of the cell death factors BNIP3 and NIX in human tumors. (ox.ac.uk)
  • Solid tumors contain regions of hypoxia, a physiological stress that can activate cell death pathways and, thus, result in the selection of cells resistant to death signals and anticancer therapy. (ox.ac.uk)
  • This study shows that genes regulating cell death can be hypoxically induced and are overexpressed in clinical tumors. (ox.ac.uk)
  • Knockdown of HIF-2alpha in neuroblastoma and glioma tumor stem/initiating cells leads to reduced levels of VEGF and poorly vascularized, highly necrotic tumors. (lu.se)
  • Tumors in which eosinophilic cells predominate were previously classified as "granular cell" carcinoma but are currently included among CCRCCs in the 2004 World Health Organization classification of renal tumors based on the presence of vasculature and genetic alterations typical of CCRCC. (medscape.com)
  • The incidence of renal cell carcinoma (RCC) has been rising steadily in Europe and the United States for the past 3 decades, with a particular rise in the proportion of small, asymptomatic tumors detected incidentally via abdominal imaging. (medscape.com)
  • The renal tumors are frequently multifocal and/or bilateral and are always of the clear cell renal cell carcinoma (CCRCC) histologic type. (medscape.com)
  • To assess the relative impact of normoxia and hypoxia on the HSC compartment, we performed gene set enrichment analysis (GSEA) of cells within HSC cluster 5 from each culture condition. (confex.com)
  • The expression of proangiogenic proteins VEGF and HIF-1 alpha, under conditions of normoxia and hypoxia, was verified using immunocytochemistry and quantified by densitometry. (fapesp.br)
  • An article Timegated® Raman spectroscopy and proteomics analyses of hypoxic and normoxic renal carcinoma extracellular vesicles is published in Nature - Scientific Reports. (timegate.com)
  • Mutations in the VHL gene are also found in the majority of sporadic clear cell renal carcinoma, the most common malignant neoplasm of the human kidney. (aspetjournals.org)
  • Choroidal endothelial cells (RF/6A) were maintained in a semiconfluent state and hypoxia was induced by exposing the cells to cobalt chloride for 24 h. (molvis.org)
  • However, its role has not been evaluated in hypoxic choroidal endothelial cells (CECs), the precursor of VEGF-mediated choroidal angiogenesis. (molvis.org)
  • The expression levels of FENDRR in hypoxic pulmonary artery endothelial cells (HPAECs) were detected by using quantitative real-time polymerase chain reaction (qRT-PCR) and fluorescence in situ hybridization (FISH). (biomedcentral.com)
  • We previously found that exosomes released from hypoxic CRC cells enhanced tumour growth and angiogenesis by enhancing the proliferation and migration of endothelial cells ( 13 ). (ijbs.com)
  • Consistent with these findings, we found that Wnt4 was enriched in exosomes released from hypoxic CRC cells and mediated the functions of endothelial cells ( 13 ). (ijbs.com)
  • Hypoxia and ischemia are linked to oxidative stress, which can activate the oxidant-sensitive transient receptor potential ankyrin 1 (TRPA1) channel in cerebral artery endothelial cells, leading to vasodilation. (elifesciences.org)
  • We hypothesized that TRPA1 channels in endothelial cells are activated by hypoxia-derived reactive oxygen species, leading to cerebral artery dilation and reduced ischemic damage. (elifesciences.org)
  • Using isolated cerebral arteries expressing a Ca 2+ biosensor in endothelial cells, we show that 4-hydroxynonenal and hypoxia increased TRPA1 activity, detected as TRPA1 sparklets. (elifesciences.org)
  • Loss of TRPA1 channels in endothelial cells increased cerebral infarcts, whereas TRPA1 activation with cinnamaldehyde reduced infarct in wildtype, but not Trpa1 ecKO, mice. (elifesciences.org)
  • G-protein-coupled receptors and ion channels present on the endothelial cells and vascular smooth muscle cells (SMCs) that form the walls of cerebral blood vessels initiate many of the signaling cascades that enable these intrinsic adaptive processes. (elifesciences.org)
  • Using cDNA arrays and serial analysis of gene expression, we found that hypoxia induces up-regulation of BNIP3 and its homologue, Nip3-like protein X. Analysis of human carcinoma cell lines showed that they are hypoxically regulated in many tumor types, as well as in endothelial cells and macrophages. (ox.ac.uk)
  • We validated the physiological relevance of this model by showing that hypoxia inducible factor-1α expression was significantly increased by IH exposure in human aortic endothelial cells, murine breast carcinoma (4T1) cells as well as in a blood-brain barrier model (2.5-, 1.5-, and 6-fold increases, respectively). (mcqinst.com)
  • Since stroke and coronary heart disease are common OSA-associated comorbidities, increased knowledge of the effects of IH exposure of endothelial cells and blood brain barrier appears to be crucial. (mcqinst.com)
  • In this study, we investigated the role of hypoxia induced SIRT1 in choroidal neovascularization in relation to age-related macular degeneration. (molvis.org)
  • Abstract Nerves are a component of the tumor microenvironment contributing to cancer progression, but the role of cells from nerves in facilitating cancer invasion remains poorly understood. (ibecbarcelona.eu)
  • inbook{ecf3c49f-5038-4d94-a89d-062e1056050f, abstract = {{Cellular adaptation to diminished tissue oxygen tensions, hypoxia, is largely governed by the hypoxia inducible transcription factors, HIF-1 and HIF-2. (lu.se)
  • ABSTRACT: Performing hypoxia-reoxygenation cycles in cell culture with a cycle duration accurately reflecting what occurs in obstructive sleep apnea (OSA) patients is a difficult but crucial technical challenge. (mcqinst.com)
  • Similarly, exposure to low oxygen conditions in vitro induced stem-cell-like potential in immature cortical GFAP + cells. (jneurosci.org)
  • For the first time, with this paper the writers Anatoliy Samoylenko, Martin Kögler, Artem Zhyvolozhnyi, Olha Makieieva, Geneviève Bart, Sampson S. Andoh, Matthieu Roussey, Seppo J. Vainio & Jussi Hiltunen show that Timegated® Raman can distinguish between the low level of oxygen deficiency (hypoxia) and normoxia of EVs and that it is a fast and far less expensive method than mass-spectroscopy, showing comparable results. (timegate.com)
  • The term hypoxia is used to describe biological situations where insufficient levels of oxygen exist. (mdpi.com)
  • but the molecular mechanisms underlying how cells adapt to variations in oxygen supply were unknown until the prize-winning work described here. (nobelprize.org)
  • Animal cells undergo fundamental shifts in gene expression when there are changes in the oxygen levels around them. (nobelprize.org)
  • Through the combined work of these three laureates it was thus demonstrated that the response by gene expression to changes in oxygen is directly coupled to oxygen levels in the animal cell, allowing immediate cellular responses to occur to oxygenation through the action of the HIF transcription factor. (nobelprize.org)
  • for example, in 1858 Louis Pasteur was the first to show that there is a complex balance of oxygen use in animal cells, and that cells use multiple pathways to accomplish energy con-version (Pasteur, 1858). (nobelprize.org)
  • In almost all animal cells, the ability to rapidly respond and adapt to variations in oxygen availability is essential. (nobelprize.org)
  • We observed enhanced progenitor cell activity within the CD34+ cell population treated with Delta1 in hypoxia, but the benefits provided by low-oxygen cultures were most notable in the primitive HSC compartment. (confex.com)
  • A total of 64 patients with renal cell carcinoma were divided into the observation group (nursing based on oxygen administration) and the control group (conventional nursing). (biomedcentral.com)
  • Nursing based on oxygen administration decreased the value of renal function indexes, serum inflammatory factors, and tumor markers in renal cell carcinoma patients. (biomedcentral.com)
  • These data reveal that nursing based on oxygen administration can improve the clinical efficacy of renal cell carcinoma therapies, being safe and effective. (biomedcentral.com)
  • The oxygen sensing and signal transduction machinery is located in the pulmonary arterial smooth muscle cells (PASMCs) of the pre-capillary vessels, albeit the physiological response may be modulated in vivo by the endothelium. (ersjournals.com)
  • It aimed to discern how hypoxia affected hNTSC characteristics, proliferation, and differentiation potential compared to hNTSCs cultured under normal oxygen levels. (bvsalud.org)
  • Progressive hypoxia resulted in a reversible suppression of Ca 2+ efflux to 90% of normoxic controls with an apparent Michaelis constant for oxygen of 145 μM. (dundee.ac.uk)
  • To minimize side effects, we designed a hypoxia-inducible CAR (HiCAR), which is driven by a hypoxia response element (HRE), and consists of a conventional CAR and an oxygen-dependent degradation domain (ODD) that is actively degraded under normoxia but stabilized under hypoxia. (biomedcentral.com)
  • Next, we evaluated the controllability of tumor cell-killing activities of HiCAR by oxygen. (biomedcentral.com)
  • Cellular adaptation to diminished tissue oxygen tensions, hypoxia, is largely governed by the hypoxia inducible transcription factors, HIF-1 and HIF-2. (lu.se)
  • In recent years, high tumor cell levels of HIF-2 and the oxygen sensitive subunit HIF-2alpha have been associated with unfavorable disease and shown to be highly expressed in tumor stem/initiating cells originating from neuroblastoma and glioma, respectively. (lu.se)
  • Following exposure to low oxygen the cells were removed from the chambers and harvested spun down and fast freezing before isolation of microRNA as 17-AAG detailed below. (biotechnologyconsultinggroup.com)
  • Control cells were cultured as above and managed in normoxic EGR1 (20 % oxygen) conditions until harvested. (biotechnologyconsultinggroup.com)
  • Intermittent hypoxia (IH) is the hallmark of obstructive sleep apnea (OSA), a common chronic disease affecting 5-20% of the general population and characterized by recurrent collapses of the upper airway, leading to the repetitive occurrence of oxygen desaturation/reoxygenation sequences. (mcqinst.com)
  • This highlights the challenge of oxygen diffusion for effective cell exposure to IH. (mcqinst.com)
  • Together our results demonstrate a new mechanism explaining hypoxia-induced resistance involving the alteration of the response to DSB in D283-MED cells, but also highlight the cell type to cell type diversity and the necessity to take into account the differing tumour genetic make-up when considering re-sensitisation therapeutic protocols. (biomedcentral.com)
  • The human renal cell carcinoma cell line A498 under hypoxia/normoxia was used as an experimental model in vitro and the biological characteristics and mitochondrial function of the cells were assessed. (biomedcentral.com)
  • The in vitro I/R model was established by the hypoxia/reoxygenation method using H9C2 cells. (spandidos-publications.com)
  • METHODS: Both AT-MSCs and UC-MSCs were obtained from an established cell bank and expanded in vitro . (minervamedica.it)
  • I demonstrated that using glucose restriction of CD8⁺ T cells in vitro led to improved tumor regression in vivo. (ubc.ca)
  • HiCAR-T cells showed enhanced cytotoxicity against tumor cells under hypoxia compared to normoxia in vitro and antitumor efficacy comparable to that of conventional CAR-T cells in vivo. (biomedcentral.com)
  • The contribution of CXCL12 to hypoxia-induced angiogenesis was examined in vivo using a subcutaneous murine model of neovascularization. (haematologica.org)
  • Over-expression of hypoxia-inducible factor in multiple myeloma plasma cells strongly induced in vivo angiogenesis, and administration of a CXCL12 antagonist decreased hypoxia-inducible factor-induced angiogenesis. (haematologica.org)
  • Conclusions Hypoxia-inducible factor-2 is a newly identified regulator of CXCL12 expression in multiple myeloma plasma cells and a major contributor to multiple myeloma plasma cell-induced angiogenesis. (haematologica.org)
  • Targeting the hypoxic niche, and more specifically hypoxia-inducible factor-2, may represent a viable strategy to inhibit angiogenesis in multiple myeloma and progression of this disease. (haematologica.org)
  • 6 , 7 CXCL12 is an important mediator of several aspects of MM biology including transendothelial migration, 8 , 9 MM plasma cell migration and retention within the bone marrow, 10 , 11 angiogenesis, 7 and osteoclastic bone resorption. (haematologica.org)
  • 14 , 15 HIF-1 and HIF-2 mediate adaptive responses to hypoxia by inducing the transcription of genes associated with erythropoiesis, glycolytic metabolism, cell survival and angiogenesis. (haematologica.org)
  • Even though the exact mechanisms underlying ischemic injury in the muscle are not completely understood, hypoxia-inducible factor-1 alpha (HIF-1α) has emerged as an attractive target to enhance post ischemic angiogenesis. (frontiersin.org)
  • Inactivation of VHL ubiquitin ligase is associated with normoxic stabilization of hypoxia-inducible factor-1α and 2-α (HIF-1α and HIF-2α), transcriptional regulators of tumor angiogenesis, invasion, survival, and glucose utilization. (aspetjournals.org)
  • Hypoxia-inducible factor 1 (HIF-1) participates in tumor angiogenesis by upregulating target genes, such as vascular endothelial growth factor (VEGF). (oncotarget.com)
  • As previously reported, colorectal cancer cell-derived exosomes have important roles in tumour progression including invasion, angiogenesis, immune modulation and distal metastasis through effectively delivering microRNAs, mRNAs and proteins ( 10 - 12 ). (ijbs.com)
  • In recent years, one of the main research strategies used to induce angiogenesis in vivo has been via stem cell therapy, especially therapy using mesenchymal stem cells (MSCs). (minervamedica.it)
  • Hypoxia induced adjustments included physiological pathways regulating the strain response angiogenesis cell and migration routine regulation. (biotechnologyconsultinggroup.com)
  • Design and Methods The expression of hypoxia-inducible factor protein was examined in patients' bone marrow biopsy specimens using immunohistochemistry. (haematologica.org)
  • Results Strong hypoxia-inducible factor-2 protein expression was detected in CD138 + multiple myeloma plasma cells in patients' biopsy specimens. (haematologica.org)
  • We found that exposure of renal adenocarcinoma cells to hypoxia induced EV secretion and led to notable changes in the EV protein cargo in comparison to normoxia. (timegate.com)
  • Using this cell line, we have identified emetine as a specific inhibitor of HIF-2α protein stability and transcriptional activity. (aspetjournals.org)
  • Without altering HIF-2α mRNA level, emetine rapidly and dramatically down-regulated HIF-2α protein expression in 786-O cells. (aspetjournals.org)
  • When cells were transfected with GFP-HIF-1α, treatment with TSA increased the number of green fluorescence protein (GFP)-positive cells. (oncotarget.com)
  • Whole cell extracts (30 µg lysate) of HeLa (Lane 1), SH-SY5Y (Lane 2), A549 (Lane 3), A-431 (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). (thermofisher.com)
  • Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. (thermofisher.com)
  • Here, I investigate the role of one intrinsic factor (receptor-type protein tyrosine phosphatase alpha) and one extrinsic factor (ionizing radiation) in facilitating cancer cell invasion. (ubc.ca)
  • Receptor-type protein tyrosine phosphatase alpha (PTPα is a widely expressed transmembrane-bound protein that has been implicated in integrin signaling, focal adhesion formation, and normal cell migration. (ubc.ca)
  • A protein called TRPA1 is found in the cells that form the inner lining of blood vessels. (elifesciences.org)
  • Regulation was hypoxia inducible factor-1-dependent, and hypoxia inducible factor-1 expression was suppressed by von Hippel-Lindau protein in normoxic cells. (ox.ac.uk)
  • Tumor hypoxia and high HIF protein levels are frequently associated with aggressive disease. (lu.se)
  • The prolyl hydroxylation of hypoxia-inducible factor 1α (HIF-1α) mediated by the EGLN-pVHL pathway represents a classic signalling mechanism that mediates cellular adaptation under hypoxia. (nature.com)
  • Hypoxia changes the cellular redox state and activates class III histone deacetylase sirtuin1 (SIRT1). (molvis.org)
  • Here, we investigated whether ex vivo culture of HSPCs under hypoxia might limit cellular ER stress and thus offer a simple approach to preserve functional HSCs under high proliferative conditions, such as those promoted in culture with Delta1. (confex.com)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • These cellular components can include cells of the innate immune system, such as myeloid-derived suppressor cells (MDSCs), tumor-associated macrophages (TAMs), and eosinophils, as well as cells of the adaptive immune system, including CD4⁺ T regulatory cells (Tregs) and cytotoxic CD8⁺ T cells. (ubc.ca)
  • Cancer cells can exploit normal mechanisms of cellular motility to facilitate invasion and metastasis, the process by which cancer cells disseminate to distant organs. (ubc.ca)
  • Here we identify RIPK1, a known regulator of cell death mediated by tumour necrosis factor receptor 1 (TNFR1), as a target of EGLN1-pVHL. (nature.com)
  • As a result, the glycolysis pathway in diabetic HepG2 cells was accelerated by the Hypoxia-Induced Factor (HIF), which was preceded by galactolysis. (pulsus.com)
  • Hypoxia is a critical pathological factor in a variety of retinal diseases, including age-related macular degeneration. (molvis.org)
  • Activated SIRT1 signals hypoxia inducible factor (HIF)-2α, which transactivates vascular endothelial growth factor (VEGF) and erythropoietin. (molvis.org)
  • The role of SIRT1 in the activation of HIF-2α and nuclear factor-κB (RelA/p65) during hypoxia in the presence or absence of SIRT1 was assessed using immunoblot analysis. (molvis.org)
  • In astrocytes the OGD enhanced the nuclear translocation of hypoxia-inducible factor 1 (HIF-1), the major transcription factor regulating EPO expression. (jneurosci.org)
  • Prolonged exposure to hypoxia strongly up-regulated CXCL12 expression in multiple myeloma plasma cells and hypoxia-inducible factor-2 was found to play a key role in this response. (haematologica.org)
  • and (v) the role of hypoxia-inducible factor-1 in lung development. (stanford.edu)
  • While much research is, and has been, carried out to investigate the effects of hypoxia, this is usually done using experimental models which employ stable levels of hypoxia. (mdpi.com)
  • Therefore, this study aimed to investigate the effects of hypoxia-preconditioned MSCs from human adipose tissue-derived MSCs (AT-MSCs) and from human umbilical cord-derived MSCs (UC-MSCs). (minervamedica.it)
  • miRNAs have been implicated as transcriptional regulators in a wide range of biological processes determining cell fate, stress response, proliferation, or death [ 5 ]. (hindawi.com)
  • Multiple myeloma (MM) is an incurable hematologic malignancy characterized by the clonal proliferation of malignant plasma cells in the bone marrow. (haematologica.org)
  • We decellularized the metastatic lung sections, measured the micromechanics of the tumor ECM, and stained the sections for ECM proteins, proliferation, and cell death markers. (ibecbarcelona.eu)
  • Our findings show that breast cancer or nontumorigenic breast cells showed high viability and cell proliferation for up to 7 days. (ibecbarcelona.eu)
  • Wnt4 promotes the proliferation of cancer stem cells in response to progesterone in breast cancer ( 20 ). (ijbs.com)
  • The investigation utilized fluorescence-activated cell sorting (FACS) for surface marker analysis, cell counting kit-8 assays for proliferation assessment, and multiplex immunoassays for cytokine secretion study. (bvsalud.org)
  • Proliferation of hNTSCs remained unaffected by hypoxia. (bvsalud.org)
  • Studies from a number of laboratories in the past decade have revealed that the central pathways deregulated in cancer often serve to coordinately regulate both classic oncogenic signaling pathways controlling cell proliferation and cell survival with pathways controlling cell metabolism. (aacrjournals.org)
  • Prolyl hydroxylation of RIPK1 mediated by EGLN1 promotes the binding of RIPK1 with pVHL to suppress its activation under normoxic conditions. (nature.com)
  • Prolonged hypoxia promotes the activation of RIPK1 kinase by modulating its proline hydroxylation, independent of the TNFα-TNFR1 pathway. (nature.com)
  • As such, inhibiting proline hydroxylation of RIPK1 promotes RIPK1 activation to trigger cell death and inflammation. (nature.com)
  • Our findings illustrate a key role of the EGLN-pVHL pathway in suppressing RIPK1 activation under normoxic conditions to promote cell survival and a model by which hypoxia promotes RIPK1 activation through modulating its proline hydroxylation to mediate cell death and inflammation in human diseases, independent of TNFR1. (nature.com)
  • Our data support the conclusion that hypoxia promotes pluripotency in GFAP + cells in the cortical parenchyma. (jneurosci.org)
  • In this study, I hypothesized that PTPα promotes invadopodia-mediated triple-negative breast cancer (TNBC) cell invasion. (ubc.ca)
  • In today's research, we elucidated a book system underpinning the effect from the lack of HTATIP2 manifestation for the activation of HIF signaling that mediates tumor version to hypoxia and consequently promotes intense tumor development and level of resistance to therapy inside a murine xenograft style of A549 human being lung adenocarcinoma, which represents the most frequent subtype of non-small cell lung carcinoma (NSCLC). (estme.org)
  • Thus, under conditions frequently established for solid tumours in vivo, such as aglycemia and hypoxia, the metabolic flexibility of cancer cells is demonstrated. (pulsus.com)
  • Fate-mapped cortical GFAP + cells derived ex vivo from hypoxic, but not normoxic, mice were able to form pluripotent, long-term self-renewing neurospheres. (jneurosci.org)
  • Notch activation in human CD34+ hematopoietic stem/progenitor cells (HSPCs) by treatment with Delta1 ligand has enabled clinically relevant ex vivo expansion of short-term HSPCs. (confex.com)
  • To corroborate these findings molecularly, we performed transcriptomic analyses and found significant upregulation of a distinct HSC gene expression signature in cells cultured with Delta1 in hypoxia (Fig. A). Collectively, these data show that hypoxia supports a superior ex vivo expansion of human HSCs with LTR activity compared with normoxia at optimized densities of Delta1. (confex.com)
  • In-vivo assessment of the angiogenic potency of hypoxic and normoxic AT-MSCs and UC-MSCs was conducted using an ex ovo quail embryo model (cultured in 6-well plates). (minervamedica.it)
  • In vivo , hypoxic preconditioning could enhance the angiogenic potency of AT-MSCs, compared to normoxic AT-MSCs, although the angiogenic potency of hypoxic and normoxic UC-MSCs was not significantly different. (minervamedica.it)
  • In contrast, ACT expands a patient's own T cells ex vivo to direct anti-tumor immunity when transfused back into the patient. (elifesciences.org)
  • In this study Happy Cell ASM® was used to improve the 3D ex-vivo expansion of patient derived CTC's. (valelifesciences.com)
  • For AT-MSCs, there was a significant increase in VEGF and bFGF production when preconditioned with hypoxia, compared to normoxia, while HGF production was only slightly increased. (minervamedica.it)
  • In D283-MED and U87MG, chronic hypoxia (5 days), but not acute hypoxia (24 h) induced resistance to chemotherapy and X-ray irradiation. (biomedcentral.com)
  • This acquired resistance upon chronic hypoxia was present but less pronounced in MEB-Med8A cells. (biomedcentral.com)
  • Fig. 1: Hypoxia induces cell death in a RIPK1-dependent manner. (nature.com)
  • Our study results demonstrate that hypoxia mimetic cobalt chloride induces SIRT1 and augments HIF-2α, which activates and releases VEGF. (molvis.org)
  • Inactivating mutations of the von Hippel-Lindau (VHL ) tumor suppressor gene are associated with inherited VHL syndrome, which is characterized by susceptibility to a variety of neoplasms, including central nervous system hemangioblastoma and clear cell renal cell carcinoma (CCRCC). (aspetjournals.org)
  • Clear cell renal cell carcinoma (CCRCC) is a renal cortical tumor typically characterized by malignant epithelial cells with clear cytoplasm and a compact-alveolar (nested) or acinar growth pattern interspersed with intricate, arborizing vasculature. (medscape.com)
  • Typical gross presentation of clear cell renal cell carcinoma (lower left) with golden color due to intracellular lipid accumulation. (medscape.com)
  • These changes in gene expression alter cell metabolism, tissue re-modeling, and even organismal responses such as increases in heart rate and ventilation. (nobelprize.org)
  • William Kaelin, Jr. was in 1995 engaged in the study of the von Hippel-Lindau tumor suppressor gene, and after isolation of the first full-length clone of the gene showed that it could suppress tumor growth in VHL mutant tumorigenic cell lines. (nobelprize.org)
  • Hypoxia was found to induce A498 cell invasion, migration, and the release of inflammatory cytokines, while repressing human solute carrier family 14 member 1 gene expression. (biomedcentral.com)
  • Overexpression of the solute carrier family 14 member 1 gene could abolish hypoxia-induced invasion, reduce the migration of A498 cells, inhibit the hypoxia-induced release of inflammatory cytokines, and arrest the cell cycle at the G1/S checkpoint. (biomedcentral.com)
  • The results elucidate a mechanism wherein the solute carrier family 14 member 1 gene participates in the occurrence and development of hypoxia-induced renal cell carcinoma in a mitochondria-dependent manner. (biomedcentral.com)
  • Adaptation to hypoxia and consequent pro-inflammatory gene expression of prostate and breast carcinomas have been implicated in the progression toward cancer malignant phenotype. (biomedcentral.com)
  • Increases in knowledge have revealed that the common pathophysiological scenario, myocardial ischemia reperfusion injury, including ischemia/reperfusion (I/R) and hypoxia/reoxygenation (H/R) forms of injury, results in depressed myocardial function and harmful morphological alterations, which may lead to heart failure ( 4 ). (spandidos-publications.com)
  • The immediate partial restoration of respiration upon addition of membrane-permeant dimethyl-2- oxoglutarate to inhibited cells demonstrated the absence of 2OGdehydrogenase substrate post aminotransferase inhibition.Surprisingly, the AOA (bithionol) inhibition stopped after 72 hours of 5% O2 hypoxia, and respiration was fully recovered. (pulsus.com)
  • In agreement with this, G9A inhibition leads to increased cell death, impaired cell migration, reduced cell cycle and downregulated anchorage-independent growth. (nih.gov)
  • Interestingly, whole transcriptome analysis revealed that a number of previously non-responsive genes become amenable to hypoxia following G9A inhibition, including many targets that drive cancer cell survival. (nih.gov)
  • This was accompanied by the upregulation of the hypoxia inducible factors HIF1A and HIF2A during the use of G9A inhibition to curb cancer cell growth even under normoxic conditions. (nih.gov)
  • Therefore, we show that G9A is a key mediator of oncogenic processes in breast cancer cells and cancer therapeutics involving G9A inhibition can be used successfully to attenuate oncogenicity. (nih.gov)
  • Inhibition of exosome secretion by GW4869 reduced hypoxic exosome-mediated migration and invasion of normoxic CRC cells. (ijbs.com)
  • RESULTS: The data showed that preconditioning with hypoxia promoted the secretion of bFGF, VEGF, and HGF by both AT-MSCs and UC-MSCs. (minervamedica.it)
  • The hypoxic regulation of CXCL12 was examined in multiple myeloma plasma cell lines using polymerase chain reaction and western blotting. (haematologica.org)
  • The role of hypoxia-inducible factors-1 and -2 in the regulation of CXCL12 expression was examined using over-expression and short hairpin RNA knockdown constructs, electrophoretic mobility shift assays and chromatin immunoprecipitation. (haematologica.org)
  • Emetine-induced HIF-2α down-regulation was confirmed in three additional VHL-renal cancer cell lines, was insensitive to the prolyl hydroxylase inhibitor dimethyloxaloyl glycine, and did not require neural precursor cell expressed developmentally down-regulated-8, suggesting that emetine accesses a previously undescribed cullin-independent proteasome degradation pathway for HIF-2α. (aspetjournals.org)
  • This is due to acute alterations in the tissues surrounding the tumor and in the environment as a result of treatment with anticancer therapeutics, which facilitate the drug resistance of tumor cells. (oncotarget.com)
  • It is largely undetectable in cells or tissues grown under normoxic conditions. (abcam.cn)
  • The aim of the present study was to analyze the cell viability-, migration and invasion, as well as the expression of proangiogenic proteins VEGF and HIF-1 alpha, in HepG2 hepatocarcinoma cells, following treatment with melatonin. (fapesp.br)
  • In Chapter 2, I examined how proteins secreted by cancer cells can promote a tumor-permissive microenvironment through the recruitment of suppressive innate and adaptive immune cells to the lungs. (ubc.ca)
  • I demonstrated that tumor cells secreted proteins that drove the recruitment and/or expansion of pulmonary TAMs, Tregs, and granulocytic-MDSCs (G-MDSCs), resulting in increased primary lung tumor growth and pulmonary metastasis of mammary carcinoma cells, respectively. (ubc.ca)
  • Targeting tumor-secreted proteins, or the suppressive immune cells that are expanded in response to said proteins, led to a decrease in primary and metastatic pulmonary disease. (ubc.ca)
  • Our results suggest that astaxanthin has a neuroprotective effect against RGC death induced by glutamate stress, oxidative stress, and hypoxia, which induce apoptotic and necrotic cell death. (molvis.org)
  • Hypoxia is considered a critical contributor to renal cell carcinoma progression, including invasion and metastasis. (biomedcentral.com)
  • Pyroptosis is a form of programmed cell death involved in the pathophysiological progression of hypoxic pulmonary hypertension (HPH). (biomedcentral.com)
  • Cancer cells release exosomes with various functions into the tumour microenvironment during cancer progression. (ijbs.com)
  • In the present study, we investigated whether TSA treatment increases HIF-1α stabilization via acetylation under normoxic conditions, which would lead to VEGF upregulation and resistance to anticancer drugs. (oncotarget.com)
  • Further, inhibitory receptors present in the TME can inhibit T cell metabolism and alter T cell signaling both directly and through release of extracellular vesicles such as exosomes. (elifesciences.org)
  • A Hepatocellular carcinoma in humans HepG2 cells are forced to undergo Oxidative Phosphorylation (OXPHOS) when grown in aglycemic conditions with galactose and glutamine.These OXPHOS cells serve as a prototype for cancer cells' combined aerobic glycolysis and OXPHOS bioenergetics. (pulsus.com)
  • 27 samples consisting of MCF7 human breast cancer cells treated with 6 μM BIX-01294 in 0.1% dimethyl sulfoxide (DMSO) or 0.1% DMSO carrier controls and untreated controls in acute (4 hours) hypoxia, chronic (24 hours) hypoxia (1% O2) or normoxia (21% O2) control conditions in triplicate cultures. (nih.gov)
  • TSA enhanced total HIF-1α and VEGF-HRE reporter activity under normoxic conditions. (oncotarget.com)
  • These data demonstrate that HIF-1α may be stabilized and translocated into the nucleus for the activation of VEGF promoter by TSA-mediated acetylation at K674 under normoxic conditions. (oncotarget.com)
  • 0.001) conditions, compared with the control group and with induced hypoxia alone. (fapesp.br)
  • 0.05 for both conditions) (Shape 1C,D). Since downregulation of HTATIP2 continues to be associated with obtained sorafenib level of resistance [28,29,30], a cytotoxicity research was completed to examine the effect of HTATIP2 Fluorescein Biotin knockdown on tumor cell response to sorafenib treatment under normoxic. (estme.org)
  • This study investigated the influence of hypoxic culture conditions on human nasal inferior turbinate-derived stem cells (hNTSCs), a subtype of mesenchymal stem cells (MSCs). (bvsalud.org)
  • In these cells, HIF-2 is active under nonhypoxic conditions as well, creating a pseudo-hypoxic phenotype with clear influence on tumor behavior. (lu.se)
  • As high HIF-2alpha expression further correlates with disseminated disease as demonstrated in neuroblastoma, glioma, and breast carcinoma, we propose that targeting HIF-2alpha and/or the pseudo-hypoxic phenotype induced by HIF-2 under normoxic conditions has great clinical potential. (lu.se)
  • Under normoxic conditions HIF-1 alpha has a short half-life. (abcam.cn)
  • Taken together, the results of our study indicate that hypoxia may stimulate tumour cells to release Wnt4-rich exosomes that are delivered to normoxic cells to enhance prometastatic behaviours, which might provide new targets for CRC treatment. (ijbs.com)
  • Chrysin regulated miR-18a, miR-21, and miR-221 genes in gastric cancer cell line [ 26 ]. (hindawi.com)
  • Using transcriptomic analysis in D283-MED cells, we found a large transcriptional remodelling upon long term hypoxia, in particular the expression of a number of genes involved in detection and repair of double strand breaks (DSB) was altered. (biomedcentral.com)
  • Hypoxic stem cell lines showed a clear time-dependent activation of the entire panel of pro-inflammatory genes as compared to differentiated tumor cells. (biomedcentral.com)
  • We have also evidenced a similar pattern of overexpressed genes in GBM-SCs after hypoxic treatment, showing also a gain of invasive and migratory function that was lost when these stem cells differentiated. (biomedcentral.com)
  • Such activation of transcription is achieved by binding of HIF-1α to hypoxia-responsive elements (HREs) located on the promoters of target genes [ 9 ]. (biomedcentral.com)
  • Moreover, exosomal Wnt4 enhanced β-catenin translocation to the nucleus in normoxic CRC cells. (ijbs.com)
  • Eltzschig, H. K., Bratton, D. L. & Colgan, S. P. Targeting hypoxia signalling for the treatment of ischaemic and inflammatory diseases. (nature.com)
  • A recent study provided evidence that pyroptosis is involved in the inflammatory process of human pulmonary artery smooth muscle cells (HPASMCs) in HPH (Zhang et al. (biomedcentral.com)
  • Furthermore, FZHY was observed to block the activation of the NLRP3 inflammasome pathway, which can lead to excessive inflammatory cytokine release in supernatants and cell lysates in response to LPS and ATP. (bvsalud.org)
  • The aim of our study was to analyze the hypoxic and pro-inflammatory microenvironment in GBMs and to demonstrate that in a stem/progenitor cell line derived from human glioblastoma (GBM-SCs), hypoxia activates a coordinated inflammatory response, evidencing an invasive and migratory phenotype. (biomedcentral.com)
  • Contrary to aglycemic cells, glycolytic HepG2 cells showed the conventional HIF-mediated adaptation when exposed to hypoxia, i.e., even with unlimited respiratory substrate availability for 72 hours at 5% O2. (pulsus.com)
  • This thesis work aims to examine the diverse phenotype and function of myeloid cells in primary and metastatic disease, ultimately contributing to an improved understanding of the interplay between the innate and adaptive immune system in the solid tumor microenvironment. (ubc.ca)
  • Neuroblastoma tumor initiating cells are immature with a neural crest-like phenotype and. (lu.se)
  • Neuroblastoma tumor initiating cells are immature with a neural crest-like phenotype and downregulation of HIF-2alpha in these cells results in neuronal sympathetic differentiation and the cells become phenotypically similar to the bulk of neuroblastoma cells found in clinical specimens. (lu.se)
  • This increase in stiffness strongly correlated with an increase in necrosis, which reveals a potential link between tumor hypoxia and ECM deposition and stiffness. (ibecbarcelona.eu)
  • Medulloblastoma (MB) is a malignant embryonal brain tumour originating from neural stem cells or granule-cell progenitors of the cerebellum, due to a deregulation of signalling pathways involved in neuronal development such as Wnt or Sonic Hedgehog (SHH) [ 1 ]. (biomedcentral.com)
  • T cells must substantially increase nutrient uptake to mount a proper immune response and failure to obtain sufficient nutrients or engage the appropriate metabolic pathways can alter or prevent effector T cell differentiation and function. (elifesciences.org)
  • Here, we found that exosomes secreted by hypoxic CRC cells promoted the migration and invasion abilities of normoxic CRC cells. (ijbs.com)
  • Exosomal Wnt4 mediated hypoxic exosome-mediated migration and invasion of normoxic CRC cells. (ijbs.com)
  • The activation of β-catenin signalling was important for the migration and invasion of normoxic CRC cells, which was eliminated by treatment with the β-catenin inhibitor ICG-001. (ijbs.com)
  • We found that exosomes released from hypoxic CRC cells enhanced the migration and invasion abilities of normoxic CRC cells. (ijbs.com)
  • The analysis of the processes of cell migration and invasion was performed in a Boyden chamber. (fapesp.br)
  • The rate of cell migration and invasion VMS additionally reduced in cells treated with 1 mkt melatonin for 48 h when compared with the control group (P=0.496). (fapesp.br)
  • During normal cell migration, cells use focal adhesions to facilitate cycles of cell adhesion to, and release from, the extracellular matrix (ECM). (ubc.ca)
  • Up coming the migration and invasion potentials of A549shNT and A549shHTATIP2 cells had been evaluated under normoxic and hypoxic circumstances using the wound-healing assay and transwell invasion assay. (estme.org)
  • 0.01 for both circumstances) (Shape 1A,B), indicating that A549shHTATIP2 cells possess higher migration potential than A549shNT cells beneath the same tradition condition. (estme.org)
  • The survival rates of tumor patients are decreasing because of tumor cell resistance to anticancer chemotherapies [ 1 ]. (oncotarget.com)
  • Hypoxic pulmonary vasoconstriction (HPV), also known as the von Euler-Liljestrand mechanism, is an essential response of the pulmonary vasculature to acute and sustained alveolar hypoxia. (ersjournals.com)
  • Hypoxia is the most common characteristic of solid tumours driving cancer metastasis. (ijbs.com)
  • Biological assays showed that invasive and migratory behavior was strengthened by hypoxia only in GBM stem cells. (biomedcentral.com)
  • Extracellular vesicles (EVs) represent a diverse group of small membrane-encapsulated particles involved in cell-cell communication, but the technologies to characterize EVs are still limited. (timegate.com)
  • My studies suggest that PTPα is an intrinsic regulator of TNBC cell invasion. (ubc.ca)
  • Calorimetric-respirometric(CR) ratios of -353 (day 3) to -333 (day 7) kJ mol -1 O 2 are consistent with embryos that are aerobically recovering from hypoxia, but are inconsistent with an ongoing anaerobic contribution to metabolism. (biologists.com)
  • The TME, however, can be metabolically hostile due to insufficient vascular exchange and cancer cell metabolism that leads to hypoxia, depletion of nutrients, and accumulation of waste products. (elifesciences.org)
  • This review will discuss the metabolic changes that drive T cells into different stages of their development and how the TME imposes barriers to the metabolism and activity of tumor infiltrating lymphocytes. (elifesciences.org)
  • As T cell metabolism dictates effector function, it is now apparent that the effect of cancer cell metabolism on the tumor microenvironment (TME) may impair anti-tumor immunity, and these new hallmarks of cancer are therefore inextricably linked. (elifesciences.org)
  • Expanded understanding of the basic biology of T cell activation has enabled immunotherapy to combat cancer, and T cell metabolism now offers the opportunity to optimize and improve these therapeutic strategies. (elifesciences.org)
  • Finally, we will provide an overview of how utilizing an understanding of T cell metabolism may inform strategies to alter the TME or enhance T cell metabolism to strengthen the immunotherapy arsenal. (elifesciences.org)
  • A hypoxia-induced mouse model of pulmonary hypertension (PH) was used to test preventive effect of conserved fragment TFO2 of FENDRR. (biomedcentral.com)
  • In contrast, during global alveolar hypoxia, HPV leads to pulmonary hypertension. (ersjournals.com)
  • CXCL12 is a constitutively expressed chemokine which binds primarily to the CXCR4 receptor and regulates cell growth, chemotaxis, myelopoiesis, lymphopoiesis, and development of the nervous and cardiovascular systems. (haematologica.org)
  • Consistent with our hypothesis, carnosine supplementation augmented HIF-1α and VEGF expression in the ischemic limb and the mobilization of proangiogenic Flk-1 + /Sca-1 + cells into circulation. (frontiersin.org)
  • In contrast, pretreatment of hypoxic C2C12 cells with methylcarcinine, a carnosine analog, lacking Fe 2+ chelating capacity, had no effect on HIF-1α levels and VEGF release. (frontiersin.org)
  • The concentrations of growth factors (such as HGF, bFGF, and VEGF) in the cell culture supernatants collected from culture media of the hypoxia-preconditioned AT-MSCs and UC-MSCs (as well as those from AT-MSCs and UC-MSCs cultured in normoxia) were measured by ELISA. (minervamedica.it)
  • The purpose of this study was to investigate the role and mechanism of hypoxia-induced renal cell carcinoma and provide evidence-based medical proof for improvements to postoperative nursing of renal cell carcinoma patients. (biomedcentral.com)
  • Hypoxic pulmonary vasoconstriction (HPV), also known as the von Euler-Liljestrand mechanism, is an intrinsic mechanism of the pulmonary vasculature in response to alveolar hypoxia, to match ventilation to perfusion and optimise pulmonary gas exchange ( figure 1 ). (ersjournals.com)
  • Overall, our study demonstrates the potential of the HiCAR for improving the safety of CAR-T cells to promote the clinical application of CAR-T immunotherapy. (biomedcentral.com)
  • Hypoxia-inducible factors (HIFs), especially HIF-1α, are largely responsible for mediating adaptive reactions to hypoxia ( 6 ). (ijbs.com)
  • The aim of this thesis work is to interrogate the pro- and anti- tumorigenic roles myeloid cells play in primary and metastatic disease, and how these myeloid populations interact with cells of the adaptive immune system to influence anti-tumor immunity. (ubc.ca)
  • Functions as a master transcriptional regulator of the adaptive response to hypoxia. (abcam.cn)
  • Cytokine expression showed similarity between hypoxic and normoxic groups throughout cultivation. (bvsalud.org)
  • In the present study, the protective effects of EGCG with zinc were assessed on cultures of rat cardiac myoblasts exposed to hypoxia/reoxygenation (H/R) injury. (spandidos-publications.com)
  • The purpose of this study was to investigate whether astaxanthin confers a neuroprotective effect against glutamate stress, oxidative stress, and hypoxia-induced apoptotic or necrotic cell death in primary cultures of rat retinal ganglion cells (RGCs). (molvis.org)
  • However, no data have yet been reported in RGC primary cultures using other stressors related to GON, such as glutamate toxicity or hypoxia. (molvis.org)
  • At optimal coating densities of Delta1, the frequency of LTR-HSCs measured by limiting dilution analysis 16 weeks after transplantation into NSG mice was 4.9- and 4.2-fold higher in hypoxic cultures (1 in 1,586 CD34+ cells) compared with uncultured cells (1 in 7,706) and the normoxia group (1 in 5,090), respectively. (confex.com)
  • We identified 6 distinct clusters (clusters 0 to 5) in dimension-reduction (UMAP) analysis, with a comparable distribution of cells per cluster between normoxic and hypoxic cultures. (confex.com)
  • TITLE: An innovative intermittent hypoxia model for cell cultures allowing fast Po2 oscillations with minimal gas consumption. (mcqinst.com)
  • In conclusion, we have established a new device to perform rapid intermittent hypoxia cycles in cell cultures, with minimal gas consumption and the possibility to expose several culture dishes simultaneously. (mcqinst.com)
  • In this context, significant efforts were recently made in several leading laboratories in the field to obtain relevant models of IH in cell cultures. (mcqinst.com)
  • H9c2 cells were subjected to 3‑h hypoxia, followed by 1‑h reperfusion. (spandidos-publications.com)
  • To the best of our knowledge, the current study was the first to demonstrate that EGCG + Zn2+ protects H9c2 cells against H/R injury through activation of the PI3K/Akt pathway, as determined by western blotting. (spandidos-publications.com)
  • leaves alleviate hypoxia/reoxygenation (H/R) injury in H9c2 cardiomyocytes and to explore potential mechanisms. (hindawi.com)
  • H9c2 cells pretreated with TFs for 24h were exposed to H/R treatment. (hindawi.com)
  • The protective effects of TFs resulted in higher expression of miR-21 in H/R-induced H9c2 cells than that of controls, which in turn upregulated Akt signaling activity via suppressing the expression of PTEN together with decreasing the ratio of Bax/Bcl-2, caspase3, and cleaved-caspase3 expression in H/R-induced H9c2 cells. (hindawi.com)
  • Moreover, we have found that TFs can increase the activity of superoxide dismutase (SOD) and reduce the level of malonaldehyde (MDA) together with lactate dehydrogenase (LDH) in H9c2 cells. (hindawi.com)
  • To test this hypothesis, we attempted to investigate whether TFs can exert its cardioprotective effect and the effects of TFs on miR-21 in H9c2 cells. (hindawi.com)
  • The number of live RGCs was then counted using a cell viability assay. (molvis.org)
  • Under hypoxia, RGC viability was reduced to 66%, and astaxanthin administration resulted in a significant increase in viability to 67%, 77%, and 93%, respectively. (molvis.org)
  • Cells were cultured and cell viability was investigated using 3-(4,5-dimethylthiazol-2-y1)-2,5-diphenyltetrazolium bromide (MIT) assay. (fapesp.br)
  • The MIT assay revealed a reduction in cell viability (P=0.018) following treatment with ntM melatonin for 24 h. (fapesp.br)
  • However, cancer cells mount a survival response via activation of the HIF pathway that may necessitate the additional suppression of HIF signaling for improved efficacy in the eradication of the disease. (nih.gov)
  • Dr. Otto Warburg first described, more than 80 years ago, that a fundamental biochemical difference between tumor cells and their normal counterparts was that tumor cells rely on aerobic glycolysis for ATP generation, unlike most normal differentiated cells of the body, which use mitochondrial oxidative phosphorylation. (aacrjournals.org)