Normal human. Medical search. Frequent questions

however, the expression in normal and malignant adult tissues has not been examined in detail. (nature.com)
Here, we examined the expression of the HE4 gene and protein in a large series of normal and malignant adult tissues by oligonucleotide microarray and tissue microarray, respectively. (nature.com)
Using tissue microarrays and full tissue sections of normal and 448 neoplastic tissues, HE4 immunoreactivity was found in normal glandular epithelium of the female genital tract and breast, the epididymis and vas deferens, respiratory epithelium, distal renal tubules, colonic mucosa, and salivary glands, consistent with HE4 gene expression. (nature.com)
Here, we used oligonucleotide and tissue microarrays to survey HE4 gene and protein expression in a large group of malignant and normal tissues from adults. (nature.com)
While this approach led to a broad repertoire of individually weak T cell responses against multiple TAAs that cumulatively resulted in strong selective pressure against immune escape, the need to inject the vectors into normal tissues often results in toxicity, they admit. (genengnews.com)
This approach, they reasoned, might activate autoimmune and antitumor T cell responses, and dispense with the need for administration directly into normal tissues. (genengnews.com)
Remnants of ancient viruses in the human genome are active in healthy tissues as well as diseased ones, limiting their utility as disease biomarkers, according to a study by Aidan Burn at Tufts University in Boston, USA and colleagues, publishing October 18th in the open access journal PLOS Biology . (scienceblog.com)
More evolutionarily ancient HML-2 viruses showed the highest expression levels in human tissues, which may indicate that the activity of younger, less-degraded HERV fragments containing complete protein-coding sequences may be repressed by cells to prevent production of harmful viral proteins, the authors say. (scienceblog.com)
John Coffin, the senior author, adds, "We have found that nearly all normal human tissues express, in their RNA, one or another of about three dozen endogenous proviruses, remnants of widespread retrovirus infection of our distant ancestors. (scienceblog.com)
Pantomics Array Description: Multi-normal/disease tissues from 12 anatomic sites, serving as controls for >90% of the IHC markers. (delos.info)
The repository covers a wide variety of species and diseases, including human adult normal tissues, human diseased and tumor tissues, as well as mouse, rat, and monkey tissues. (biochain.com)
Cytoplasmic Proteins from different human adult normal tissues are available. (cytomol.com)
Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane.The human normal tissues included in the blot are left cerebellum, right cerebellum, frontal lobe, occipital lobe, parietal lobe, whole eye, spinal cord, temporal lobe, and thalamus. (caspase-14.com)
Description: 33 types of normal tissues based on the FDA recommendation for antibody cross-reactivity testing. (caspase-14.com)
Human and animal tissues provide different possibilities for eruption analyses, briefly discussed in the introduction. (hindawi.com)
Tooth eruption has been examined in both animal and human tissues. (hindawi.com)
This is not possible in human tissues. (hindawi.com)
This methodology is expected to allow investigation of important ECM-based hypotheses in human tissues and permits future scientific exploration in an organ- and disease-specific manner. (atsjournals.org)
But for the most part, normal tissues and 9 cell lines). (lu.se)

Human epididymis protein 4 (HE4) was first identified in the epithelium of the distal epididymis and originally predicted to be a protease inhibitor involved in sperm maturation. (nature.com)
Chromosome 21 aneuploid cells constitute ∼4% of the estimated one trillion cells in the human brain and include non-neuronal cells and postmitotic neurons identified by the neuronspecific nuclear protein marker. (jneurosci.org)
Much knowledge about AD comes from studies of transgenic rodents expressing mutated human amyloid-β protein precursor (AβPP) to increase Aβ production or the Aβ42/40 ratio. (lu.se)
Description: The human tissue blot is designed for proteomics research in screening for antibody and protein expression. (caspase-14.com)
It carries 9 different human normal tissue lysates with 15 µg of total cellular protein in each lane. (caspase-14.com)

In a series of 175 human adult tumors, gene expression was highest in ovarian serous carcinomas. (nature.com)
Researchers have developed a virus-based vaccine expressing a broad spectrum of undefined antigens from normal human prostate tissue that they claim completely eradicates established mouse prostate tumors in experimental animals. (genengnews.com)
The team's previous work had already shown that killing normal cells in situ using the adjuvant hsp70 generated T cell responses to antigens that mediated rejection of tumors of the same histological type. (genengnews.com)
They then went on to test the virus-expressed human cDNA library in mice with established prostate tumors. (genengnews.com)
In addition, recurrent tumors expressed genes associated with an epithelial-to-mesenchymal-type transition, and had lost, or at least reduced, expression of mouse homologs of the human RNAs encoding prostate-specific antigens in the ASEL. (genengnews.com)
An integrated genomics analysis of epigenetic subtypes in human breast tumors links DNA methylation patterns to chromatin states in normal mammary cells. (lu.se)

Immunohistochemistry method was used to detect the expression of ALDH1 in 160 cases of IDC, 50 cases of ADH, 20 cases of UDH and 22 normal duct cases. (omicsonline.org)

By using human lungs and cells, this new system provides a novel way to culture cells in vitro to investigate matrix contributions to lung pathology in a disease-specific manner. (atsjournals.org)
Western blot shows lysates of A172 human glioblastoma cell line, A549 human lung carcinoma cell line, and HeLa human cervical epithelial carcinoma cell line. (rndsystems.com)

Here we show that micro-injection of normal human fibroblasts with an antibody directed against the p53-binding domain of mdm2 induces expression of p53-responsive genes, and furthermore results in p53-dependent growth arrest. (soton.ac.uk)
Description: This recombinant Human IgG1 antibody reacts to the heavy chain of hIgG1. (caspase-14.com)
Human Antibody Laboratories manufactures the normal human igg1 antibody reagents distributed by Genprice. (caspase-14.com)
The Normal Human Igg1 Antibody reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (caspase-14.com)
Description: A polyclonal antibody for detection of IgG1 from Human. (caspase-14.com)
Description: A Rabbit Polyclonal antibody against IgG1 from Human. (caspase-14.com)
Description: A Rabbit polyclonal antibody against Human Normal Mucosa Of Esophagus Specific 1 (NMES1). (caspase-14.com)
ProSci Type: Tissue (Normal) Tested Application: IHC Application: ProSci's human tissue slides can be used with any antibody shown to be. (agtcbioproducts.com)
PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human Sirtuin 1/SIRT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7714) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016 ). (rndsystems.com)
HepG2 human hepatocellular carcinoma cell line was stained with Sheep Anti-Human Sirtuin 1/SIRT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7714, filled histogram) or isotype control antibody (Catalog # 5-001-A , open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127 ). (rndsystems.com)

In normal skin all epithelial cells were glutamate and, apparently more weakly, aspartate immunoreactive. (nih.gov)
Normal human bronchial epithelial cells (NHBE) were grown in culture and exposed to hog confinement dust extract. (cdc.gov)

In direct ELISAs, less than 1% cross-reactivity with recombinant human SIRT2 is observed. (rndsystems.com)
To further demonstrate the importance of IL-6 in the regulation of TLR2, NHBE were directly stimulated with recombinant human IL-6. (cdc.gov)

HE4 is one of more than 8900 different human genes represented on U95a GeneChips (Affymetrix, Santa Clara, CA, USA), which we used previously to develop a molecular classification of 175 human carcinomas based on patterns of gene expression. (nature.com)
Viral infection of sperm or egg cells can result in viral genes being permanently incorporated into the host genome, and the genetic remnants of ancient viruses - known as human endogenous retroviruses (HERV) - make up about 8% of the human genome. (scienceblog.com)
Although no longer infectious, some HERVs still contain intact genes, and the production of HERV RNA transcripts in human cells has been linked to some cancers. (scienceblog.com)
687 genes differentiated tumor samples before and after treatment, among which 310 genes did not show the same differential expression in the placebo group or normal samples. (karger.com)
Within the HCM tissue , differentially expressed genes between areas of normal and severe disarray were enriched for genes related to mitochondrial energetics and the extracellular matrix in severe disarray. (bvsalud.org)
Explore the normal functions of human genes and the health implications of genetic changes. (medlineplus.gov)

Nevertheless, building on the positive aspects of this approach, the researchers postulated that it may be possible to provide a broad antigenic repertoire for in vivo immune selection of relevant tumor-associated antigens by expressing a cDNA library of a normal tissue using a systemically delivered, immunogenic vector. (genengnews.com)
Tissue samples were taken from the tumor and from normal adjacent mucosa during both colonoscopy and surgery. (karger.com)
Methods: Primary breast tumor-derived CAFs were transduced to express human telomerase reverse transcriptase (hTERT) and sorted into CD105 low and CD105 high populations using fluorescence-activated cell sorting (FACS). (lu.se)

The presence of glutamate/aspartate-like immunoreactivity was studied in normal human skin and in skin with gold-induced inflammation. (nih.gov)

Of 144 CIN2/3 lesions, preceding normal smears showed hrHPV positivity in 80% of cases. (bmj.com)

The team cloned a cDNA library from normal human prostate, which they termed altered self antigen and epitope library (ASEL) into VSV, and systemically injected the antigen-expressing vector into normal experimental mice. (genengnews.com)

P. M. Frossard, M. J. Malloy, G. G. Lestringant and J. P. Kane, "Haplotypes of the Human Renin Gene Associated with Essential Hypertension and Stroke," Journal of Human Hypertension, Vol. 15, No. 1, 2001, pp. 49-55. (scirp.org)

However, therapeutic specificity may depend on the extent to which this p53-inhibitory action of mdm2 is also required by normal cells. (soton.ac.uk)
Western Blot Shows Human Sirtuin 1/SIRT1 Specificity by Using Knockout Cell Line. (rndsystems.com)

These results point out hCG and Ang-(1 - 7) as effective compounds to inhibit cell proliferation, since they decrease cell viability and increase apoptosis in both normal and in tumoral breast cells, being the effect more pronounced in the tumoral cell line. (scirp.org)
Background: Human breast cancer most frequently originates within a well-defined anatomical structure referred to as the terminal duct lobular unit (TDLU). (lu.se)

A single human adult normal tissue with 5-10 µm thickness is mounted on a positively charged glass slide. (biochain.com)

In addition to the WHO workforce data as at 31 July 2022, made available on the WHO website on 8 November 2022,1 this report provides an update as at July 2022 of the trends in the workforce and of related activities with respect to the three pillars of the WHO human resources strategy: attracting talent, retaining talent and fostering an enabling working environment. (who.int)
A report on human resources covering the full calendar year 2022 will be provided to the Seventy-sixth World Health Assembly. (who.int)

The presence of high-risk human papillomavirus (hrHPV) is associated with an increased risk of subsequent high-grade cervical intra-epithelial neoplasia (CIN2/3) and cervical cancer. (bmj.com)

METHODS: Aβ42 immunolabeling of 12 Wistar rat brains (3-18 months of age) and brain sections from six human subjects. (lu.se)

Together, these data demonstrate that human brain cells (both neurons and non-neuronal cells) can be aneuploid and that the resulting genetic mosaicism is a normal feature of the human CNS. (jneurosci.org)
Toward determining the presence of constitutional aneuploidy in the human brain, we report here an analysis of the frequency of chromosome 21 gain and loss among neurons and non-neuronal cells isolated from the cerebral cortex and hippocampus of normal individuals ranging from 2 to 86 years of age. (jneurosci.org)

Metaphase spread of normal human male chromosomes, showing Y-banding. (wellcomecollection.org)

Here, using double-label fluorescence in situ hybridization for the autosome chromosome 21 (chromosome 21 point probes combined with chromosome 21 "paint" probes), along with immunocytochemistry and cell sorting, we present evidence for chromosome gain and loss in the human brain. (jneurosci.org)
Fresh-frozen samples of postmortem brain tissue from normal cerebral cortices and hippocampi were obtained from the Harvard Brain Bank (United States Public Health Service MH/NS 31862) and from the University of Miami/University of Maryland Brain and Tissue Bank (National Institute of Child Health and Human Development NO1-HD-8-3284). (jneurosci.org)
OBJECTIVE: To characterize the brain-wide expression of Aβ42 throughout the life span of outbred Wistar rats, and to relate these findings to brains of human subjects without neurological disease. (lu.se)
The human brain has a short attention span, and higher-level thinking drops off very quickly when capacity is reached. (asisonline.org)

This study aims to explore its mechanism by defining changes in gene expression between neoplastic and normal tissue samples before and after treatment. (karger.com)

Loss of function of the tumour suppressor gene p53 is a key event in most human cancers. (soton.ac.uk)
Genetically unmatched human Ipsc and Esc Exhibit Equivalent gene expression and neuronal differentiation potential. (ixcellsbiotech.com)

This process results in a more physiologic substrate that retains normal stiffness, architecture, and matrix composition compared with native lungs. (atsjournals.org)
depicts humans who have undergone physiologic changes, developing intolerance for the sun and a unique form of communication while maintaining the ability to learn through mimicry and form social hierarchies. (cdc.gov)

The distribution of this molecule in normal and pathological synovia from patients with osteoarthritis (OA) and rheumatoid arthritis (RA) was investigated by indirect immunofluorescence utilizing specific monoclonal antibodies. (nih.gov)
Animal studies and studies on normal and pathological eruption in humans can support and explain different aspects in the new theory. (hindawi.com)
Meanwhile, the eruption process has been studied longitudinally in extensive studies in humans with focus on both normal and pathological conditions. (hindawi.com)

N* = upper limit of normal for LDH assay. (medscape.com)

The intensity of glutamate and especially aspartate-like immunoreactivities seemed to be increased in the epidermis and dermis of the inflamed as compared to the normal skin, and this increase was particularly pronounced in the HLA-DR positive (dendritic) cells in the epidermis. (nih.gov)
Moreover, the macrophage-like cells in the human skin may have a role in the processing of glutamate and aspartate on a recycling basis. (nih.gov)
ALDH1 was expressed in a few cells in most samples except in a few cases of ADH, UDH and normal ducts, in which ALDH1 expression showed extensive distribution. (omicsonline.org)
The score of ALDH1 in luminal epithelial cytoplasm expression is 3, 2, and 1 while myoepithelial cells and stroma expression are 3, 2, and 2 in ADHÃ¯Â¼ÂŒUDH and normal ducts respectively. (omicsonline.org)
Further cholinergic signalling during stress has been reported to increase bowel ion secretion in humans and uptake of HRP in rodents via activation of mast cells. (projectsparadise.com)
The overall aim: of this thesis was to examine the role of CRH-mediated and cholinergic signalling, and their interaction with mast cells and eosinophils, in the regulation of the mucosal barrier function in the normal human colon and in UC. (projectsparadise.com)
The recent discovery of induced pluripotent stem cells (iPSCs) not only overcomes the ethical and logistical issues associated with human embryonic stem cells, but also provides a flexible platform for generating various differentiated cell types from diseased individuals.iPSC-derived NSCs are a potentially valuable source of in vitro models for complex, polygenic human diseases, and are potentially useful for drug discovery and cell-based therapy applications [2] . (ixcellsbiotech.com)
iXCells Biotechnologies provides high quality human neural stem cells (NSCs) derived from normal or diseased iPS cell lines. (ixcellsbiotech.com)
Cells can further expand for 3-5 passages in Human Neural Stem Cell Growth Medium (Cat# MD-0024), but they are not recommended for extensive expansion, because the purity of the neural stem cell population may decrease. (ixcellsbiotech.com)

By clearing landscapes to make way for farmland or build cities, humans destroy huge swaths of natural habitats that serve as a home to these species. (smithsonianmag.com)
The disappearance of plant species fits into a broader wave of extinctions caused by human activity. (smithsonianmag.com)
The growing utilization of IVF will transform the way a substantial proportion of the human species procreates. (ivf-concern.am)

however, the relevance to humans is not known. (jneurosci.org)

Redesigning the nursing and human resource partnership: A model for the new normal era. (belitungraya.org)

Furthermore, comprehensive analysis of acellular matrix ECM details significant compositional differences between normal and fibrotic lungs, paving the way for further study of novel hypotheses. (atsjournals.org)
Fast Analysis of Human Normal Immunoglobulin using CElixirPro3. (mtc-usa.com)
Spatial Transcriptomic Analysis of Focal and Normal Areas of Myocyte Disarray in Human Hypertrophic Cardiomyopathy. (bvsalud.org)
To identify the focal and spatially restricted alterations in the transcriptional pathways and reveal novel therapeutic targets, we performed a spatial transcriptomic analysis of the areas of focal myocyte disarray compared to areas of normal tissue using a commercially available platform (GeoMx, nanoString). (bvsalud.org)
Histological sections were reviewed by an expert pathologist , and 72 focal areas with varying degrees of myocyte disarray (normal, mild, moderate, severe) were chosen for analysis . (bvsalud.org)
A deconvolution analysis utilizing the matched single nuclei RNA -sequencing ( snRNA -seq) data to determine cell type composition in areas of interest revealed significant differences in fibroblast and vascular cell composition in areas of severe disarray when compared to normal areas in HCM samples. (bvsalud.org)

We conclude that normal cell proliferation can be dependent on negative regulation of p53 by mdm2, a finding which raises an important note of caution for mdm2-directed cancer therapies. (soton.ac.uk)
Areas of interest were interrogated with the Human Cancer Transcriptome Atlas designed to profile 1800 transcripts. (bvsalud.org)

HML-2 ORF Transcripts in the Human Body: This map of the human body (adapted from the GTEx consortium website with permission) displays the possible expressed Human endogenous proviral HML-2 ORFs in each tissue sampled. (scienceblog.com)

Varindra shared the new models the companies might adapt to where remote work culture scenes may become the new normal in human capital space . (uknowva.com)

Researchers claim systemic vaccination with broad antigenic repertoire from normal prostate stimulates anticancer immune response but not autoimmunity. (genengnews.com)

The functional role of glutamate and aspartate in normal skin, and the significance of the increase in the levels of these amino acids in several cell populations in the inflammatory skin is not known, but modulatory or protective roles may be considered. (nih.gov)
Tenascin was found in normal synovium just beneath the whole lining cell layer. (nih.gov)
Sirtuin 1/SIRT1 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in Sirtuin 1/SIRT1 knockout HeLa cell line. (rndsystems.com)
Detection of Sirtuin 1/SIRT1 in HepG2 Human Cell Line by Flow Cytometry. (rndsystems.com)
Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and SIRT1 knockout HeLa cell line (KO). (rndsystems.com)
Cell composition in the normal areas of the control tissue was also divergent from the normal areas in HCM samples, which was consistent with the differential expression results. (bvsalud.org)

Reproductive medicine, and especially IVF , is rapidly transforming human reproduction and is thus bound to remain of fundamental importance to both science and society. (ivf-concern.am)

To study the differences of ALDH1 expression in Invasive Ductal Carcinoma (IDC), Atypical Ductal Hyperplasia (ADH), Usual Ductal Hyperplasia (UDH) and normal ducts and their clinical significance. (omicsonline.org)
Yet, little is known about the normal expression of Aβ42 in rodent brains. (lu.se)

Human studies, mainly clinical and radiological, have focused on normal eruption and gender differences. (hindawi.com)
Both clinical and radiological studies have been performed in humans and animals. (hindawi.com)

Tissues19

Protein5

Tumors6

Immunohistochemistry1

Lung2

Antibody10

Epithelial cells2

Recombinant human2

Genes6

Tumor3

Inflammation1

Lesions1

CDNA1

20011

Specificity2

Breast2

Thickness1

20222

Papillomavirus1

Methods1

Neurons2

Metaphase1

Brain4

Neoplastic1

Gene2

Physiologic2

Pathological3

Assay1

Cells9

Species3

Relevance1

Redesigning1

Analysis6

Cancer2

Transcripts1

Adapt1

Systemic1

Cell6

Reproductive1

Expression2

Clinical2