Nonhistone nuclear protein. Medical search. Frequent questions

HMGB1 is a member of the high-mobility group (HMG) chromosomal protein family [28]. (pkc-inhibitor.com)
HMGB1 (High-mobility group box-1) protein was originally described as a nuclear non-histone DNA binding chromosomal protein. (thermofisher.cn)
The high mobility group protein HMGB1 is the most abundant non-histone chromosomal protein in mammals and has counterparts in all eukaryotes. (edu.hk)
The AT-hook is a small DNA-binding protein motif which was first described in the high mobility group non-histone chromosomal protein HMG-I(Y). Since its discovery, this motif has been observed in other DNA-binding proteins from a wide range of organisms. (embl.de)

None of the 4 sera contained antibodies to DNA, histones, RNA, DNA histone complexes, or nonhistone chromosomal proteins. (eurekamag.com)
Acetylation of histones controls access of regulatory proteins to the DNA. (moodcoaching.nl)
Protéines histone vs nonhistone: Les protéines histones sont le principal composant protéique de la chromatine. (moodcoaching.nl)
Histones undergo posttranslational modifications that alter their interaction with DNA and nuclear proteins. (moodcoaching.nl)
Complex patterns of posttranslational modifications of chromatin including acetylation of histones establish combinatorial signals that are apparently interpreted by chromatin-binding proteins and the gene expression machinery to control transcriptional activity of individual genes. (aacrjournals.org)
DNA-binding domains, zinc fingers, leucine zippers) and histones and nonhistone proteins interacting with chromatin, all of which can influence gene expression. (cambridgeproteinarrays.com)

Adding chemical groups to the DNA backbone and modifying histone proteins impart distinct characteristics on chromatin architecture. (frontiersin.org)
Although originally termed histone acetyltransferases (HATs) for their lysine acetylation activity on histone N-terminal tails, CBP and its paralogue, p300, have been shown to acetylate a variety of non-histone proteins including p53, DNA polymerase β and nuclear import factors. (enzolifesciences.com)
Set7 is also associated with regulation of different non-histone proteins including nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB). (edu.au)
Nucleosomes are an example of chromatin structure, where you've got DNA wrapped around a core of histone proteins. (moodcoaching.nl)
Reversible acetylation of histone proteins is considered to affect local structure of chromatin. (aacrjournals.org)

HMGB1, a highly conserved non-histone nuclear protein, is widely expressed in mammalian cells. (bvsalud.org)
High-mobility group box 1 (HMGB1) is a nuclear protein that can also act as an extracellular trigger of inflammation, proliferation, and migration in eye diseases. (pkc-inhibitor.com)
Several studies indicate that ocular diseases have a close relationship with autoimmune reactions and inflammatory responses [2C5], and a key protein in such processes is high-mobility group box 1 (HMGB1) [6C8]. (pkc-inhibitor.com)
HMGB1 is a DNA-binding nuclear protein discovered over 30 years ago [9C11]. (pkc-inhibitor.com)
HMGB1 was identified as a nonhistone chromatin-binding protein about 30 years ago [9C11]. (pkc-inhibitor.com)
Two nuclear localization sequences in HMGB1 may stabilize the chromatin structure and regulate gene transcription. (pkc-inhibitor.com)
HMGB1 is a protein that belongs to the High Mobility Group-box superfamily. (arigobio.cn)
HMGB1 is a DNA binding protein. (arigobio.cn)
Western blot: 100 ng of HMGB1 and HMGB2 recombinant proteins stained with ARG66714 anti-HMGB1 Neutralizing antibody [SQab20175] (low endotoxin). (arigobio.cn)
ELISA: 2 µg/ml dilution of recombinant human HMGB1, bovine HMGB1 and recombinant human HMGB2 proteins (left to right) were coated onto ELISA plate. (arigobio.cn)
A recombinant fusion protein containing a sequence corresponding to amino acids 1-215 of human HMGB1. (thermofisher.cn)
HMGB1 also act as DNA nuclear binding protein that has recently been shown to be an early trigger of sterile inflammation in animal models of trauma-hemorrhage via the activation of the Toll-like receptor 4 (TLR4) and the receptor for the advanced glycation endproducts (RAGE). (thermofisher.cn)
In a parallel experiment, a p53 expression plasmid was also included to find out if the p53 protein may influence the regulatory effect of HMGB1 on these promoters. (edu.hk)

Can be used to study CBP kinetics and regulation, for screening for inhibitors or activators and for radiolabeling of proteins or peptides with, for example [ 3 H]-Acetyl CoA. (enzolifesciences.com)

HMGA Proteins" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (uchicago.edu)
This graph shows the total number of publications written about "HMGA Proteins" by people in this website by year, and whether "HMGA Proteins" was a major or minor topic of these publications. (uchicago.edu)
Below are the most recent publications written about "HMGA Proteins" by people in Profiles. (uchicago.edu)
DNA-binding motifs, first described in one of the HMGA PROTEINS: HMG-I(Y) PROTEIN. (lookformedical.com)

Here, we summarize the different types of epigenetic enzymes which target corresponding protein domains, emphasize DNA methylation , histone modifications, and microRNA-mediated cooperation with epigenetic modification, and highlight recent achievements in developing targets for epigenetic inhibitor therapy. (frontiersin.org)
To test our hypothesis that histone H4R3 and H3R17 methylation regulates ferritin transcription, H4R3 and H3R17 protein arginine (R) methyltransferases 1 and 4 (PRMT1 and PRMT4) were investigated. (embl.de)
Arsenic exposure of human HaCaT keratinocytes induced nuclear accumulation of PRMT1 and PRMT4, histone H4R3 and H3R17 methylation proximal to the ARE, but not to the non-ARE regions of ferritin genes. (embl.de)
We describe the residue-specific methylation of E2F-1 by the asymmetric dimethylating protein arginine methyltransferase 1 (PRMT1) and symmetric dimethylating PRMT5 and relate the marks to different functional consequences of E2F-1 activity. (embl.de)
The Tudor domain protein p100-TSN reads the symmetric methylation mark, and binding of p100-TSN downregulates E2F-1 apoptotic activity. (embl.de)
Five friends of methylated chromatin target of protein-arginine-methyltransferase[prmt]-1 (chtop), a complex linking arginine methylation to desumoylation. (embl.de)
KMT1 family methyltransferases regulate heterochromatin-nuclear periphery tethering via histone and non-histone protein methylation. (instem.res.in)

Histone deacetylases (HDAC) reverse the acetylation of histone and nonhistone proteins and thereby modulate chromatin structure and function of nonhistone proteins. (aacrjournals.org)

Profiling of nucleic acid binding on HuProt human proteome microarrays can reveal interactions with the largest collection of individual human proteins available on a single slide. (cambridgeproteinarrays.com)
Nucleic acid-protein interactions regulate many aspects of gene expression and thus cell physiology and pathology. (cambridgeproteinarrays.com)
A wide range of nucleic acid binding proteins is represented on the HuProt array (see protein coverage ). (cambridgeproteinarrays.com)
The method for studying protein - nucleic acid interactions on HuProt arrays resembles that for protein-protein interactions and can lead to identification of novel binding proteins, complementing other physical and genetic methods and contributing to understanding of the functional pathways of nucleic acid interactomes. (cambridgeproteinarrays.com)
Similar to array-based protein-protein interaction screening, the purified nucleic acid of interest is either fluorescently labelled for direct detection on the array or alternatively, a biotinylated nucleic acid can be detected by secondary incubation with fluorophore-labelled streptavidin. (cambridgeproteinarrays.com)
Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus. (uams.edu)

Acetylation on lysine residues in the nuclear localization signals (NLS 1 and NLS 2) leads to cytoplasmic localization and subsequent secretion (By similarity). (arigobio.cn)
Post-translational modification of proteins at lysine residues by reversible acetylation is catalyzed by the opposing activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs), which act on both histone and non-histone substrates despite their names. (biomedcentral.com)

Antioxidant genes such as ferritin are transcriptionally activated in oxidative stress via the antioxidant responsive element (ARE), to which nuclear factor-E2-related factor 2 (Nrf2) binds and activates transcription. (embl.de)
Using RNA sequencing and gene set enrichment analysis (GSEA), Set7 inhibitors upregulated the expression of genes involved in the control of cholesterol Sterol Regulatory Element-Binding Proteins (SREBP), cholesterol and fatty acyl biosynthesis. (edu.au)
Characterisation of a cluster of genes encoding Theileria annulata AT hook DNA-binding proteins and evidence for localisation to the host cell nucleus. (lookformedical.com)
JMJD2A, through maintaining the protein stability of β-catenin, affects the transcriptional activity of β-catenin and the expression of its target genes, and this is independent of the demethylase activity of JMJD2A. (tmc.edu)
Furthermore, these genes were classified into 11 functional groups including transcription factors, cell cycle related factors, apoptosis regulators, kinases and metabolism-related proteins, etc. (edu.hk)

A nuclear and cytoplasmic protein that associates with KINETOCHORES and contains a C-terminal TUDOR DOMAIN. (bvsalud.org)

Proteins containing AT-HOOK MOTIFS that are rich in arginine and glycine residues. (uchicago.edu)
Residues 28 - 44 and 180 - 185 contain a nuclear localization signal (NLS). (thermofisher.cn)

Application of recombinant bST is a biotechnology in which a recombinant-derived protein is administered by injection to the recipient animal without changing the animal's genetic composition or genome. (nationalacademies.org)

This protein is abundantly expressed in nearly all eukaryotic cells [26]. (pkc-inhibitor.com)
We have classified these motifs into three types according to their sequence similarity and have found that they are prevalent in many eukaryotic nuclear proteins in single or multiple copies. (embl.de)

Epstein, A.L. and Clevenger, C.V., Identification of nuclear antigens in human cells by immunofluorescence, immunoelectron microscopy, and immuno-biochemical methods using monoclonal antibodies. (neobiotechnologies.com)
Monoclonal antibodies directed toward nuclear antigens are increasingly used as tools to obtain valuable information concerning the proliferative changes of the tumors. (thejcdp.com)

As part of its charge, the committee was asked to prepare a subreport evaluating methods for detecting potential unintended compositional changes across the spectrum of messenger ribonucleic acid (mRNA), proteins, metabolites and nutrients that may occur in food derived from cloned animals that have not been genetically modified via genetic engineering methods. (nationalacademies.org)

Silent information regulator 2 (Sir2) proteins, or sirtuins, are protein deacetylases dependent on nicotine adenine dinucleotide (NAD) and are found in organisms ranging from bacteria to humans. (biomedcentral.com)

The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. (abbkine.com)

This information is useful for the analyzing genetic relatedness of proteins and species. (lookformedical.com)

The feasibility of using human autoimmune antibodies to isolate and characterize specific nuclear ribonucleoprotein (nRNP) complexes was investigated. (eurekamag.com)

Recently, several chromatin-related proteins have been shown to regulate ESC pluripotency and/or differentiation, yet the role of the major heterochromatin proteins in pluripotency is unknown. (biomedcentral.com)
Here we identify Heterochromatin Protein 1β (HP1β) as an essential protein for proper differentiation, and, unexpectedly, for the maintenance of pluripotency in ESCs. (biomedcentral.com)

Many of these proteins are regulators of gene expression. (embl.de)

Formalin-fixed, paraffin-embeddedhuman tonsil stained with Pan-Nuclear AntigenRecombinant Mouse Monoclonal Antibody (rNM106). (neobiotechnologies.com)
SDS-PAGE Analysis Purified Pan-Nuclear AntigenRecombinant Mouse Monoclonal (rNM106). (neobiotechnologies.com)

Alternative splicing results in multiple transcript variants that encode the same protein. (abbkine.com)

There were no polypeptides in the isolated antigen which corresponded in MW to the core proteins of heterogeneous nuclear ribonucleoprotein (hnRNP) particles described by other investigators. (eurekamag.com)
The Nop56/58-fibrillarin heterocomplex is a core protein complex of the box C/D ribonucleoprotein particles that modify and process ribosomal RNAs. (cornell.edu)

The assembly of PRMT6 protein network allowed us to formulate functional hypotheses which led to the discovery of new molecular partners for the architectural transcription factor HMGA1a, a known substrate for PRMT6, and to provide evidences for a modulatory role of HMGA1a on the methyltransferase activity of PRMT6. (embl.de)
Egg extracts, in which the cell cycle state can be manipulated, contain stockpiles of nuclear proteins (including condensin and cohesin) sufficient for the assembly of thousands of nuclei per microliter. (nih.gov)
HMG domain proteins: architectural elements in the assembly of nucleoprotein structures. (embl.de)

The protein moiety of this fraction consisted of 2 quantitatively major polypeptides of MW 30,000 (P30) and 13,000 (P13). (eurekamag.com)
Artificial Zinc Finger Proteins with Modified Metal Moiety: Toward the Nano-Biodevices and Artificial Transcriptional Regulation. (asiaresearchnews.com)

Furthermore, AT-hook motifs are frequently associated with known functional domains seen in chromatin proteins and in DNA-binding proteins (e.g. histone folds, homeodomains and zinc fingers). (embl.de)

This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. (abbkine.com)
The knowledge of cell proliferative activity and its cellular proteins, which are involved in the control of cell proliferation, are essential components for understanding the biology of tumors. (thejcdp.com)

HMG-1 Polyclonal Antibody detects endogenous levels of HMG-1 protein. (abbkine.com)

p300/CBP acetylations play regulatory roles in transcription, DNA repair and replication, the cell cycle, p53 turnover, and nuclear import. (enzolifesciences.com)
Peroxisome proliferator-activated receptor-γ(PPARγ) is a member of the nuclear receptor superfamily of ligand-activated transcription factors [ 10 ]. (biomedcentral.com)
DNA-protein interactions occur via a range of binding motifs present in transcription factors and nuclear receptors (e.g. (cambridgeproteinarrays.com)
It has long been known as an architectural transcription factor involved in gene activation as well as in other nuclear processes. (edu.hk)
HMG-box domains are found in one or more copies in HMG-box proteins, which form a large, diverse family involved in the regulation of DNA-dependent processes such as transcription, replication, and strand repair, all of which require the bending and unwinding of chromatin. (embl.de)

Taken together, our results suggest an association of SP100 and its variants with the chromatin compartment and, further, indicate that SUMO modification may play a regulatory role in the functional interplay between the nuclear bodies and chromatin. (st-andrews.ac.uk)

High mobility group (HMG) box domains are involved in binding DNA, and may be involved in protein-protein interactions as well. (embl.de)

These mice express a catalytically inactive fusion protein of the NH 2 -terminal part of HDAC2 and β-galactosidase, which fails to integrate into corepressor complexes with mSin3B. (aacrjournals.org)

Here we report the isolation of a further cDNA, encoding the SP100C protein, that contains the PHD-bromodomain motif characteristic of chromatin proteins. (st-andrews.ac.uk)

Using pattern searches and position-dependent matrices, we have extracted the AT-hook motifs present in a non-redundant protein sequence database. (embl.de)

For RNA, hundreds of RNA binding proteins (RBPs) have now been identified which interact with mRNAs, noncoding RNAs, microRNAs etc. and are involved in post-transcriptional gene regulation including gene silencing by RNAi. (cambridgeproteinarrays.com)

For cohesion assays, demembranated sperm nuclei are incubated in interphase extract, where they undergo rapid and synchronous DNA replication and cohesion establishment through the recruitment of proteins and other factors (e.g., nucleotides) from the extract. (nih.gov)

In exploring the functional relationships between NBs and chromatin, we have shown previously that SP100 interacts with members of the HP1 family of nonhistone chromosomal proteins and that a variant SP100 cDNA encodes a high-mobility group (HMG1/2) protein. (st-andrews.ac.uk)

Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human CREB Binding Protein (CREBBP) in Tissue homogenates and other biological fluids. (hudsen.org)
Description: A sandwich ELISA for quantitative measurement of Rat CREB binding protein(CREBBP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (hudsen.org)

These characteristics correlate with a distinct nuclear architecture, epigenetic signatures enriched for active chromatin marks and hyperdynamic binding of structural chromatin proteins. (biomedcentral.com)
The binding RNA and protein to anti-nRNP columns was greatly reduced by treating the crude antigen with pancreatic RNase A before chromatography. (eurekamag.com)
Specific protein-binding measures are often used as assays in diagnostic assessments. (lookformedical.com)
Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). (arigobio.cn)
AT-hook motifs identified in a wide variety of DNA-binding proteins. (embl.de)
In general, it appears that the AT-hook motif is an auxiliary protein motif cooperating with other DNA-binding activities and facilitating changes in the structure of the DNA either as a polypeptide on its own [e.g. (embl.de)
It is most interesting that this motif seems to be quite specific to known or predicted chromosomal/DNA-binding proteins, suggesting that it may act as a versatile minor groove tether. (embl.de)

A fraction of the bound antigen, comprising less than 0.15% of the total nuclear protein, was isolated in antigenically active form. (eurekamag.com)

Transcriptional regulation of the human ferritin gene by coordinated regulation of Nrf2 and protein arginine methyltransferases PRMT1 and PRMT4. (embl.de)

However, because the A. fulgidus Nop56/58 protein lacks some domains found in most other species, it was thought that the bipartite architecture of the heterocomplex was not likely a general phenomenon. (cornell.edu)

Here, we show that Akirin2, an evolutionarily conserved nuclear protein, bridges NF-κB and the chromatin remodeling SWI/SNF complex by interacting with BRG1-Associated Factor 60 (BAF60) proteins as well as IκB-ζ, which forms a complex with the NF-κB p50 subunit. (cnrs.fr)

PRMT6 is a protein arginine methyltransferase that has been implicated in transcriptional regulation, DNA repair, and human immunodeficiency virus pathogenesis. (embl.de)
Chromatin target of Prmt1 (Chtop) is a vertebrate-specific chromatin-bound protein that plays an important role in transcriptional regulation. (embl.de)
The two NE proteins PML and SP100 are covalently modified by the ubiquitin-related SUMO-1 modifier, and recent work indicates that this modification is critical for the regulation of NE dynamics. (st-andrews.ac.uk)

Commonly observed structural components of proteins formed by simple combinations of adjacent secondary structures. (lookformedical.com)

5FMC is a nuclear complex that can only be recruited by Chtop when the latter is arginine-methylated by Prmt1. (embl.de)
In our study, we showed that JMJD2A is essential in mediating activation of the canonical Wnt/β-catenin pathway, a highly conserved and complex signaling cascade that ultimately leads to nuclear accumulation of β-catenin. (tmc.edu)
Nuclear β-catenin serves as a transcriptional coactivator by forming a transcriptional complex with TCF to activate Wnt target gene expressions. (tmc.edu)
The Mre11/Rad50/Nbs1 protein complex plays central enzymatic and signaling roles in the DNA-damage response. (ox.ac.uk)
The previous crystal structure of the Archaeoglobus fulgidus complex revealed a symmetric dimer of two Nop56/58-fibrillarin complexes linked by the coiled-coil domains of the Nop56/68 proteins. (cornell.edu)

Anatomy32

Organisms10

Diseases1

Chemicals and Drugs107

Analytical, Diagnostic and Therapeutic Techniques and Equipment36

Phenomena and Processes80

Disciplines and Occupations1

Information Science3

Health Care2

Chromosomal protein4

Histones6

Histone proteins5

HMGB113

Peptides1

HMGA Proteins4

Methylation7

Chromatin structure1

Nucleic6

Acetylation2

Genes5

Cytoplasmic1

Residues2

Recombinant1

Eukaryotic2

Antigens2

Ribonucleic Acid1

Humans1

Regulates1

Genetic1

Antibodies1

Heterochromatin2

Regulators1

Monoclonal2

Encode1

Ribonucleoprotein2

Assembly3

Moiety2

Zinc fingers1

Cellular2

Endogenous1

Transcription5

Regulatory1

Interactions1

Mice1

Isolation1

Motifs present1

Gene regulation1

Replication1

HMG11

CREB2

Binding7

Antigen1

Methyltransferases1

Species1

Factor1

Regulation3

Structural1

Complex5