Nnos protein. Medical search. Frequent questions

Human nNOS shares about 55% amino acid sequence identity with eNOS and iNOS. (rndsystems.com)
The effect of nNOS-AS was greater than that of eNOS-AS. (unboundmedicine.com)
Western immunoblot analysis revealed increased eNOS, MyoD, and myogenin protein levels 48- hours post-strain injury, but a decrease in nNOS protein levels. (cdc.gov)
Although nNOS expression was decreased, eNOS expression increased providing support for other NOS isoforms in muscle repair. (cdc.gov)
The expression of caspase‑3, Bax, Bcl‑2, phosphorylated (p)‑p38MAPK/total (t‑)p38MAPK and p‑endothelial e nitric oxide synthase (eNOS)/t‑eNOS proteins were determined by western blotting. (spandidos-publications.com)
Behavioral tests assessed sexual performance while animal tissues were used to examine eNOS and nNOS protein and mRNA in central and peripheral sites known to control erectile function. (uwo.ca)
In addition, eNOS and nNOS mRNA was increased in penile tissue and nNOS staining was increased in the paraventricular nucleus of the hypothalamus with acute treatment of ethanol extract. (uwo.ca)
Also, nNOS was increased in penile tissue with chronic treatment and eNOS with acute treatment. (uwo.ca)

The relative number of neuronal nitric oxide synthase (nNOS)-immunoreactive (IR) cells was significantly higher in IANX rats compared to sham-operated and N-propyl-L-arginine (NPLA)-treated IANX rats. (iasp-pain.org)
However, recent studies have provided evidence that mere presence of nitric oxide synthesizing-enzyme neuronal nitric oxide synthase (nNOS) in the nerve terminals may not be adequate for inhibitory nitrergic neuromuscular transmission in the gut ( 11 , 12 ). (frontiersin.org)
It is established that neuronal NO synthase (nNOS) is ubiquitinated and proteasomally degraded. (aspetjournals.org)
Objective: Nitric oxide synthase associated protein (NOS1AP) is an adaptor protein regulating neuronal functions through NMDAR/nNOS-dependent pathway. (diabetesjournals.org)
Bladder sections were stained with Masson's trichrome for SM content and immunofluorescence staining for nerve terminals expressing vesicular acetylcholine transporter (VAChT), tyrosine hydroxylase (TH), and neuronal nitric oxide synthase (nNOS). (lu.se)
Neuronal nitric oxide synthase (nNOS) and vitamin D receptor (VDR) expression were measured, as well as ER stress markers, including glucose-regulated protein78 (GRP78), protein kinase-like endoplasmic reticulum kinase (PERK) phosphorylation, eukaryotic initiation factor 2 alpha(eIF-2 alpha) phosphorylation, and CCAAT enhancer-binding protein homologous protein (CHOP). (cheric.org)
We find that these cells contain exclusively neuronal NO synthase (nNOS) isoforms as well as both NO- (soluble guanylyl cyclase, sGC) and natriuretic peptide- (natriuretic peptide receptor-A, NPR-A) responsive cGMP-producing enzymes. (nova.edu)
PIN (protein inhibitor of neuronal nitric oxide synthase), also called DLC8, is an 8 kDa cytoplasmic protein that is a light chain of the dynein molecular motor. (novusbio.com)
The sustained high concentration of intracellular calcium constitutively activates a host of enzymes, notably the calcium-activated proteases calpains, neuronal nitric oxide synthase (nNOS) and NADPH oxidase (NOX), to antagonise the cell survival signalling pathways and induce cell death. (edu.au)
Right atrial/SAN pacemaking tissue was injected with adenovirus encoding enhanced green fluorescent protein (control vector) or neuronal NO synthase (nNOS). (ox.ac.uk)
The neurotransmitter functions of nitric oxide are dependent on dynamic regulation of its biosynthetic enzyme, neuronal nitric oxide synthase (nNOS). (johnshopkins.edu)
Jaffrey, SR & Snyder, SH 1996, ' PIN: An associated protein inhibitor of neuronal nitric oxide synthase ', Science , vol. 274, no. 5288, pp. 774-777. (johnshopkins.edu)
Targeted disruption of the neuronal nitric oxide synthase (nNOS) gene results in dilated stomachs and hypertrophied pyloric sphincter muscles, a phenotype that resembles diabetic gastroparesis. (grantome.com)

nNOS is one of three NOS enzymes that catalyze the oxidation of L-argine to L-citruline and nitric oxide. (rndsystems.com)
A thesis is proposed here, based on rational argument that depletion of the cytoskeletal organizer protein shank3 may result in defective nNOS membrane localization, resulting in defective nitric oxide synthesis. (frontiersin.org)

Intrathecal injection of nNOS antisense oligonucleotides (nNOS-AS) inhibited the increase of Fos protein and NMDA(1A)R mRNA expression in the rat spinal cord during morphine withdrawal and decreased the scores of morphine withdrawal symptoms. (unboundmedicine.com)
TY - JOUR T1 - NO mediated increase of Fos protein and NMDA1A R mRNA expression in rat spinal cord during morphine withdrawal. (unboundmedicine.com)
7-Nitroindazole decreased nNOS mRNA expression and inducible (i) NOS protein levels, but had no effect on endothelial NOS expression. (tum.de)
L-arginine supplementation increased mRNA expression of all NOS isoforms, but only increased protein expression of iNOS. (tum.de)
We will study nNOS protein mRNA expression in tissue preparations and cultured enteric neurons to establish the molecular basis for enteric nervous system (ENS) nNOS down-regulation in diabetes. (grantome.com)

Spermidine inhibits NOS1 (nNOS). (botanicure.com)

Each vial contains 400µg of rabbit anti-Protein Inhibitor of nNOS (PIN) (24-58) IgG in 0.01M PBS containing 0.03% sodium azide lyophilized form. (phoenixbiotech.net)
The HPLC purified standard peptide, protein inhibitor of nNOS (24-58) can be detected by rabbit anti-Protein Inhibitor of nNOS (PIN) (24-58) IgG at 1:1000 in our dot blot system. (phoenixbiotech.net)
Dorsomorphin dihydrochloride is a potent inhibitor of AMP-activated protein kinase (AMPK) (K i = 109 nM). (tocris.com)
This inhibitor, designated PIN, appears to be one of the most conserved proteins in nature, showing 92 percent amino acid identity with the nematode and rat homologs. (johnshopkins.edu)

Honokiol inhibits phosphorylation of Akt, p44/42 mitogen-activated protein kinase (MAPK), and src. (wikipedia.org)
Dorsomorphin dihydrochloride also inhibits bone morphogenetic protein (BMP) type I receptors (ALK2, ALK3 and ALK6), promotes cardiomyogenesis in mouse embryonic stem cells (ESCs) in vitro and promotes neural differentiation of hPSCs as part of a chemical cocktail. (tocris.com)
By means of a yeast two-hybrid screen, a 10-kilodalton protein was identified that physically interacts with and inhibits the activity of nNOS. (johnshopkins.edu)
A combination treatment of Simvastatin (Simva) and Temozolomide (TMZ) inhibits autophagy flux and induces unfolded protein response (UPR) in GBM cells (U251, U87). (mdpi.com)

Following extensive washing, a Phospho-AMPKα (Thr172) Mouse Detection Antibody is added to detect the captured phospho-AMPKα (Thr172) protein. (cellsignal.com)

At least 4 isoforms of human nNOS are known. (rndsystems.com)

Diverse organ systems reveal that nNOS remains membrane-bound during enzymatic synthesis, suggesting that membrane localization of nNOS may be critical for enzyme action in a physiological context ( 5 , 24 - 33 ). (frontiersin.org)
Treatment of nNOS with N G -nitro- l -arginine or 7-nitroindazole led to stabilization of the dimeric nNOS and decreased proteasomal degradation of the enzyme, consistent with that observed in cells. (aspetjournals.org)
The PathScan ® Phospho-AMPKα (Thr172) Chemiluminescent Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of phospho-AMPKα (Thr172) protein with a chemiluminescent readout. (cellsignal.com)
Polybacterial Periodontal Pathogens Alter Vascular and Gut BH4/nNOS/NRF2-Phase II Enzyme Expression. (ox.ac.uk)

Acute administration of naloxone and chronic administration of morphine did not change the expression of Fos protein and NADPH-d positive neurons, and there was no expression of Fos/NADPH-d double-labeled neurons in the spinal cord of rats. (unboundmedicine.com)
Morphine withdrawal increased the expression of Fos protein, NADPH-d positive, and Fos/NADPH-d double-labeled neurons, and they were observed in all the laminae of the rat spinal cord. (unboundmedicine.com)
RESULTS: An increase in myogenin transcripts and a decrease in nNOS transcript expression was observed 48- hours post-strain injury by RT-PCR. (cdc.gov)
Western blots measured M2 and M3 protein expression. (lu.se)
Protein and gene expression of M3 was decreased 3 and 7 days post-BPNI, whereas M2 was unchanged. (lu.se)
Lipopolysaccharide (LPS) can regulate the expression of apoptotic factors, including caspase‑3, Bcl‑2 and Bcl‑2‑associated X protein (Bax). (spandidos-publications.com)
Protein expression was determined by Western blot and immunohistochemistry. (gla.ac.uk)
Castration decreased nNOS and α-actin expression and increased collagen expression, p38 (Thr180/Tyr182) phosphorylation, as well as caspase 3 cleavage. (gla.ac.uk)
Our study showed that treated with appropriate concentration of active vitamin D could decrease the number of pathological pyramidal neurons, improve hippocampal nerve metabolism, and reduce the over-expression of nNOS, along with the relieved activation of ER stress in hippocampus of diabetic rats. (cheric.org)
NG108-15 cells were used to characterize the expression of NO- and cGMP-generating proteins and to identify potential alterations associated with neuronal differentiation (neurite outgrowth). (nova.edu)
However, we found no differences in nNOS/BH4 protein expression in stomach tissues of infected and sham-infected mice. (ox.ac.uk)
Using enhanced green fluorescent protein, we observed transgene expression within both tissue sections and isolated pacemaking cells. (ox.ac.uk)
Adenoviral nNOS increased right atrial nNOS protein expression and cGMP content. (ox.ac.uk)
Initial results demonstrate that nNOS protein expression is increased in the presence of insulin. (grantome.com)
We will use cultured enteric neurons, primary cortical neurons and cerebellar granule cells as models to determine the role of insulin, insulin growth factors (IGFs) and their receptors in nNOS expression and regulation in the ENS and CNS. (grantome.com)

The metabolism-based inactivation of nNOS and the inhibition of heat shock protein 90 (hsp90)-based chaperones, which are known to regulate nNOS, both lead to enhanced proteasomal degradation of nNOS. (aspetjournals.org)
We found that the inactive monomeric heme-deficient nNOS (apo-nNOS) is rapidly degraded in vitro, consistent with the finding that both metabolism-based inactivation and inhibition of hsp90-based chaperones cause the formation of apo-nNOS and enhance its degradation in vivo. (aspetjournals.org)
The results of the present study demonstrate that selective inhibition of nNOS has no effect on renal injury, indicating that nNOS does not play a central role in the pathophysiology of renal I/R. In addition, although L-arginine has no effect on renal I/R injury in the model used in the present study, its administration increases the mRNA expression of NOS isoforms. (tum.de)
Biofilm prevention, inhibition and controlling process (antibiofilm) using natural or synthetic peptides derived from 2S-sulfur-rich seed storage protein 2-like protein or their peptidomimetic (synthetic) derivatives. (univ-rennes.fr)

The present study explored the effect of NAC on LPS‑induced apoptosis of HUVECs and determined the participation of the p38 mitogen‑activated protein kinase (MAPK) pathway in the process of apoptosis. (spandidos-publications.com)

Our nNOS polyclonal, recombinant monoclonal and monoclonal antibodies are created in Rabbit, Goat and Mouse. (novalabb.com)

Excitotoxic production of ROS elevates death-associated protein kinase (DAPK) activity, which provokes neuronal apoptosis in cerebral ischemia and seizure models 8 . (nature.com)
A series of caspases proteins have been shown to be activated by the death induced signal complex [a form of death ligand that binds to the corresponding death receptor on the cell surface ( 13 )], which in turn induces apoptosis. (spandidos-publications.com)

Reflux of Endoplasmic Reticulum proteins to the cytosol inactivates tumor suppressors. (univ-rennes.fr)

Since oxidative stress activates protein kinase D1 (PKD1) in tumor cells, we investigated the effect of excitotoxicity on neuronal PKD1 activity. (nature.com)
Protein kinase D1 (PKD1), together with PKD2 and PKD3, constitute a family classified within the calcium/calmodulin-dependent protein kinase superfamily 7 . (nature.com)
Role of AMP-activated protein kinase in mechanism of MetF. (tocris.com)

Unexpectedly, we find that excitotoxicity provokes an early inactivation of PKD1 through a dephosphorylation-dependent mechanism mediated by protein phosphatase-1 (PP1) and dual specificity phosphatase-1 (DUSP1). (nature.com)

Decreased calcium current after cardiac nNOS gene transfer contributes to the normalization of beta-adrenergic hyperresponsiveness in the SAN from hypertensive rats by modulating cyclic nucleotide signaling. (ox.ac.uk)

The overstimulated ionotropic glutamate receptors exert their neurotoxic effects in part by overactivation of calpains, which induce neuronal death by catalyzing limited proteolysis of specific cellular proteins. (edu.au)
The activation of ligand-gated ionotropic P2X receptors and G protein-coupled metabotropic P2Y receptors has been reported to induce an increase of intracellular Ca2+ concentration ([Ca2+]i) in inner hair cells (IHCs), outer hair cells (OHCs), spiral ganglion neurons (SGNs), and supporting cells in the cochlea. (sagepub.com)

Thus, it seems that the dimeric structure is a major determinant of nNOS stability and proteolysis. (aspetjournals.org)

Mechanistically, it shares the molecular pathways engaged by other caloric restriction mimetics: It induces protein deacetylation and depends on functional autophagy. (botanicure.com)
We sought to examine the mechanism of periodontal infection leading to altered vascular and gastrointestinal smooth muscle relaxation, focusing on the BH4/nNOS pathways. (ox.ac.uk)
This suggests that a polybacterial infection can cause significant changes in the vascular and colonic BH4/nNOS/NRF2 pathways which might lead to impaired vascular relaxation and colonic motility. (ox.ac.uk)
Limb-girdle muscular dystrophy (LGMD) protein defects occur in several pathways involved in the biologic function of muscle and can be divided into groups based on cellular localization. (medscape.com)

Minimal model for signal-induced Ca2+ oscillations and for their frequency encoding through protein phosphorylation. (arasysperfector.com)

will be … Control of endocytosis by clathrin, adaptor proteins , dynamin, and hsc70 also uses ATP. (arasysperfector.com)

The mechanism of this selective proteolytic degradation, or in essence how the nNOS becomes labilized and recognized for ubiquitination and subsequent degradation, has not been determined. (aspetjournals.org)

Antibodies that distinguish nNOS can be utilized in a few logical applications, including Western Blot, Immunohistochemistry (Paraffin), Immunocytochemistry, ELISA and Immunohistochemistry (Frozen). (novalabb.com)
These antibodies have been checked by Cell treatment and Relative articulation to affirm explicitness to nNOS. (novalabb.com)
Browse 1 of 17 nNOS antibodies, which have been approved in tries different things with 176 distributions and 119 pictures highlighted in our information display. (novalabb.com)

This involves silencing genes, blocking their production of RNA and protein. (doctorsaredangerous.com)

Detects human nNos in direct ELISAs and Western blots. (rndsystems.com)

As a polyphenol it is relatively small and can interact with cell membrane proteins through intermolecular interactions like hydrogen bonding, hydrophobic interactions, or aromatic pi orbital co-valency. (wikipedia.org)
For example, shank proteins are located further away from the membrane in the cytosolic faces of the PSDs, facing the actin cytoskeleton. (frontiersin.org)
The rationale of this organization may be related to important roles of these proteins as "exchange hubs" for the signaling proteins for their migration from the subcortical cytosol to the membrane. (frontiersin.org)
In this review, the hypothesis is forwarded that nNOS delivered to subcortical cytoskeleton requires interactions with scaffolding proteins prior to docking at the membrane. (frontiersin.org)
Dynein light chain LC8-nNOS from acto-myosin Va is possibly exchanged with shank, which thereafter facilitates transposition of nNOS for binding with palmitoyl-PSD95 at the nerve terminal membrane. (frontiersin.org)
The sGC beta(1) subunit (unlike protein phosphatase 2A subunits) is highly membrane-associated. (nova.edu)
Membrane concentrations of NPR-A and nNOS, but not sGC beta(1) protein are up-regulated with neuronal differentiation. (nova.edu)

Though human biopsy samples of functional bowel disorders are difficult to obtain for obvious reasons, animal studies have provided ambiguous results regarding concentrations of nNOS in nerve terminals in pathological states ( 9 , 10 ), which phenotypically manifest as either failure of relaxation of intestinal smooth muscles or varying degrees of impairment of gastrointestinal transit ( 11 , 12 ). (frontiersin.org)

The gross anatomic disturbance in nNOS-/- mice suggests that NO may regulate gastropyloric function. (grantome.com)

In the current study, we discovered that destabilization of the dimeric nNOS, as determined by measuring the SDS-resistant dimer, is sufficient to trigger ubiquitin-proteasomal degradation. (aspetjournals.org)
Binding of PIN destabilizes the nNOS dimer, a conformation necessary for activity. (johnshopkins.edu)

Figure 1: Relationship between protein concentration of lysates from phosphatase-treated and H 2 O 2 -treated C2C12 cells and immediate light generation with chemiluminescent substrate is shown. (cellsignal.com)
19,61) protein phosphatase I,(20) calcineurin. (arasysperfector.com)

The relative number of nNOS-IR cells encircled by GFAP-IR cells was significantly increased in IANX rats. (iasp-pain.org)

NE-stimulated cAMP concentration and L-type calcium current were also attenuated by adenoviral nNOS, along with the chronotropic responsiveness to NE in hypertensive rat atria. (ox.ac.uk)

Reassembling green fluorescent protein for in vitro evaluation of trans-translation. (univ-rennes.fr)

Evidence has suggested the role of motor proteins like myosin Va in transposition of nNOS within the nerve terminals to the membranes to facilitate nitrergic neurotransmission ( 11 , 12 ). (frontiersin.org)
TH nerve terminals surrounding the detrusor decreased in all BPNI groups, whereas VAChT and nNOS terminals decreased 14 and 30 days post-BPNI. (lu.se)

Preliminary results demonstrate that diabetic and nNOS-/- mice exhibit similar physiologic phenotypes. (grantome.com)

These include proteins associated with the sarcolemma (see image below), proteins associated with the contractile apparatus (see image below), and various enzymes involved in muscle function. (medscape.com)

Spermidine binds and precipitates DNA and may be used for purification of DNA binding proteins. (botanicure.com)

Specific protein function and abnormalities are discussed below with each LGMD. (medscape.com)

Engineered resonance energy transfer (RET) between a mTurquoise2 fluorescent protein and FMN-bound As LOV2 increases switch sensitivity. (nature.com)
This generalises to other fluorescent protein tags and As LOV2-based actuators. (nature.com)

It has been recently reported that motor proteins like myosin Va play important role in transcytosis of nNOS. (frontiersin.org)
We will also ascertain the role of nNOS in gastropyloric dysfunction of diabetic mice. (grantome.com)

Following L-arginine injection into the TG, the MHWT was significantly reduced within 15 min, and the mean number of TG cells encircled by glial fibrillary acidic protein (GFAP)-IR cells was substantially higher. (iasp-pain.org)

In the current study, we used a crude preparation of reticulocyte proteins, which contains ubiquitin-conjugating enzymes and the proteasome, to determine how nNOS is labilized. (aspetjournals.org)

Except in specific cases where protein translocation or other visualised downstream phenomena are targeted, it is hard to know whether an actuator has been switched. (nature.com)

Idiopathic MPHD subgroup showed the largest increase for the ΔHt SDS and ΔPAH SDS during GH treatment, and organic MPHD subgroup had the smallest mean increase after GH treatment, depending on ΔIGF-1 SDS and ΔIGF binding protein-3 (IGFBP-3) SDS. (bvsalud.org)

Anatomy2

Organisms1

Chemicals and Drugs6

Analytical, Diagnostic and Therapeutic Techniques and Equipment1

Information Science1

ENOS8

Synthase13

Nitric oxide2

MRNA expression5

NOS11

Inhibitor4

Inhibits4

Antibody1

Isoforms1

Enzyme4

Expression15

Inhibition4

MAPK1

Recombinant1

Apoptosis2

Endoplasmic reticulum1

Kinase3

Specificity1

Gene1

Receptors2

Proteolysis1

Pathways4

Phosphorylation1

Adaptor1

Mechanism1

Antibodies3

Genes1

ELISAs1

Membrane7

Concentrations1

Regulate1

Dimer2

Phosphatase2

Rats1

Calcium1

Vitro1

Terminals2

Demonstrate1

Sarcolemma1

Purification1

Abnormalities1

Fluorescent2

Role2

Cells1

Study1

Specific1

Treatment1