• Nitric oxide (NO) produced by endothelial nitric-oxide synthase (eNOS) represents an antithrombotic and anti-atherosclerotic principle in the vasculature. (aspetjournals.org)
  • Background- Although, endothelial nitric oxide (NO) synthase (eNOS) is believed to antagonize vascular remodeling induced by the angiotensin II (AngII) type-1 receptor, the exact signaling mechanism remains unclear. (ahajournals.org)
  • 4-6 Also, gene delivery of the key enzyme, endothelial NO synthase (eNOS), prevented atheroscrelosis and postangioplasty restenosis in vivo. (ahajournals.org)
  • The purpose of this study was to determine whether human neutrophils express an endothelial-type nitric oxide synthase (eNOS), and to study the effect of tumor necrosis factor-alpha (TNF-alpha) on its expression. (onlinejacc.org)
  • Nitric oxide (NO) is released from the endothelium through the activity of an initially defined constitutive enzyme called "endothelial-type nitric oxide synthase" (eNOS) (1-3) . (onlinejacc.org)
  • Given the role of nitric oxide in the vascular system, we aimed to test hypotheses of synergy between the common endothelial nitric oxide synthase (eNOS) Asp 298 allele and T2DM in predisposing to acute myocardial infarction (AMI). (biomedcentral.com)
  • Endothelial nitric oxide synthase (eNOS) variants were previously demonstrated in cardiovascular disease. (semanticscholar.org)
  • The impact of T786C and G894T polymorphisms of eNOS on vascular endothelial growth factor serum levels in type 2 diabetes patients. (semanticscholar.org)
  • However, the endothelial isoform (eNOS), which is significantly associated with caveolae in different cell types, has been implicated in synaptic plasticity and is enriched in the dendrites of CA1 hippocampal neurons. (frontiersin.org)
  • Consistent with previous results that indicated nitric oxide production in response to the neurotrophin BDNF, we could detect eNOS in immunoprecipitates of the BDNF receptor TrkB while nNOS could not be detected. (frontiersin.org)
  • Taken together, our results show that eNOS is located at excitatory synapses where it could represent a source for NO production and thus, the contribution of eNOS-derived nitric oxide to the regulation of neuronal survival and function deserves further investigations. (frontiersin.org)
  • Endothelial nitric oxide synthase (eNOS) gene is responsible for the bioavailability of nitric oxide and endothelial function. (cdc.gov)
  • To assess the association of the endothelial nitric oxide synthase (eNOS) (T786C and G894T) single nucleotide polymorphisms with Egyptian type 2 diabetes mellitus and diabetic nephropathy. (cdc.gov)
  • eNOS G894T variant may increase risk of type 2 diabetes with lack of association between eNOS T786C, eNOS G894T and DN in Egyptians. (cdc.gov)
  • Nitric oxide produced by the constitutive eNOS isoform controls blood flow and is a key mediator of the pro-angiogenic effects of vascular endothelial growth factor (VEGF) (16). (exposed-skin-care.net)
  • Functionally significant polymorphisms in endothelial nitric oxide synthase (eNOS) and reduced vascular eNOS activity have been associated with increased human diabetic nephropathy (DN), but the pathogenic role of eNOS deficiency in the development of DN has not yet been confirmed. (asnjournals.org)
  • In summary, eNOS −/− db/db mice provide the most robust model of type II DN that has been described to date and support a role for deficient eNOS-derived NO production in the pathogenesis of DN. (asnjournals.org)
  • Inhibition or genetic deletion of endothelial NO synthase (eNOS, NOS III) induces opening of the interendothelial junctions and increases vascular permeability in microvascular beds, suggesting that NO production may play an important role in regulating the endothelial barrier function ( 7 , 8 ). (asnjournals.org)
  • Polymorphisms in the endothelial nitric oxide synthase (eNOS) gene have been associated with the development of diabetic retinopathy (DR) in patients with type 1 diabetes mellitus (T1DM), but not with T2DM. (cdc.gov)
  • Association between eNOS 4b/a polymorphism and the risk of diabetic retinopathy in type 2 diabetes mellitus: a meta-analysis. (cdc.gov)
  • Association of VEGF and eNOS gene polymorphisms in type 2 diabetic retinopathy. (cdc.gov)
  • Background: Nitric oxide (NO), a vasodilator molecule, is produced from L-arginine by endothelial nitric oxide synthase (eNOS) and it regulates endothelial function. (journalcra.com)
  • The relationship of the endothelial nitric oxide synthase (eNOS) and vascular endothelial growth factor (VEGF) gene polymorphism in Turkish type 2 diabetic patients with and without diabetic foot ulcers. (ege.edu.tr)
  • Endothelium-derived nitric oxide (NO) is an important factor in vasodilation synthesized by endothelial nitric oxide synthase (eNOS). (wayne.edu)
  • It is produced by the enzyme endothelial nitric oxide synthase (eNOS). (garvan.org.au)
  • In the present study we aimed to evaluate whether erythropoietin (EPO) treatment may exert non-hematopoietic endothelial protection against tumor necrosis factor alpha (TNF-α) induced microvascular inflammation and to determine the involvement of the nitric oxide (NO)-producing enzyme isoforms endothelial nitric oxide synthase (eNOS) and inducible nitric oxide synthase (iNOS). (uzh.ch)
  • In liver cirrhosis, down-regulation of endothelial nitric oxide synthase (eNOS) has been implicated as a cause of increased intrahepatic resistance. (wiley.com)
  • Endothelial Nitric Oxide Synthase (eNOS) is unique among the NOS isoforms in its being dually acylated by the fatty acids myristate and palmitate. (ucm.es)
  • Chronic psychological stress cause erectile dysfunction (ED). Considering recent evidence that tumor necrosis factor-α (TNF-α) levels are increased in serum of patients with ED, the present study investigated the effects of infliximab (a TNF-α blocker) on endothelial nitric oxide synthase (eNOS) and neuronal NOS (nNOS) immunoreactivity of rat penile corpus cavernosum in unpredictable chronic mild stress (UCMS). (nature.com)
  • Erectile dysfunction in young non-obese type II diabetic Goto-Kakizaki rats is associated with decreased eNOS phosphorylation at Ser1177. (nature.com)
  • Cytokines and bacterial lipopolysaccharides (LPSs) stimulate nitric oxide production in macrophages by inducing transcription of the gene coding for the inducible isoform of nitric oxide synthase (iNOS). (nih.gov)
  • NG-Monomethyl-L-arginine, a competitive inhibitor of nitric oxide synthase activity, enhances iNOS gene mRNA induction and promoter activation by IFN-gamma and LPS, indicating that nitric oxide can influence negatively the reponsiveness of this gene to inducers. (nih.gov)
  • The effects of various respirable dusts on inducible nitric oxide synthase (iNOS) gene was analyzed to determine if there is a difference in the ability each dust to increase expression of the iNOS gene in alveolar macrophages (AM) and neutrophils. (cdc.gov)
  • To determine the role of inducible nitric oxide synthase (iNOS)-induced NO in this lung reaction, the responses of inducible nitric oxide synthase knockout (iNOS KO) versus C57BL/6J wild-type (WT) mice to aspirated LPS + IFN-gamma were compared. (cdc.gov)
  • The primary objective is to demonstrate that the plasma inducible nitric oxide synthase (iNOS) assay (PliNOSa® test) has an acceptable relative risk ratio for predicting the onset of sepsis within 72 hours of testing when performed on the first day a patient is admitted or transferred to the intensive care unit (ICU) and is considered to be at risk of becoming septic. (clinicaltrials.gov)
  • The PliNOSa® test measures inducible nitric oxide synthase (iNOS) in plasma and uses a pre-determined iNOS cut-off value to identify patients at risk for the onset of the sepsis pathology. (clinicaltrials.gov)
  • Using the skin circulation, our aim was to determine whether inducible NO synthase (iNOS) upregulation attenuates NO-dependent cutaneous vasodilation in hypertensive humans. (biomedsearch.com)
  • Microdialysis fibers served as control, iNOS inhibited (1400 W), neuronal NO synthase inhibited (N(ω)-propyl-l-arginine), and nonselective NOS inhibited (N(G)-nitro-l-arginine methyl ester). (biomedsearch.com)
  • OBJECTIVE Impaired cardiovascular function in diabetes is partially attributed to pathological overexpression of inducible nitric oxide synthase (iNOS) in cardiovascular tissues. (diabetesjournals.org)
  • Previous studies from our lab have demonstrated significant improvements in cardiovascular function of STZ-induced diabetic rats treated with the antioxidant N-acetylcysteine in parallel with inhibition of PKCβ 2 activation ( 6 ) and reduction in inducible nitric oxide synthase (iNOS)-mediated nitrosative stress ( 11 ). (diabetesjournals.org)
  • Activation of inducible nitric oxide synthase (iNOS) and oxidative stress have been shown to be associated with compromised cardiovascular function in streptozotocin (STZ)-induced type 1 diabetes. (ubc.ca)
  • The aim of the project is to investigate cardiovascular abnormalities in a rat model of type 2 diabetes (Zucker diabetes fatty or ZDF rats) and two models of metabolic syndrome (fructose-fed rats and Zucker obese rats), and to provide direct evidence linking iNOS and oxidative stress to abnormal cardiovascular function in these disorders. (ubc.ca)
  • Oxidative stress is associated with both abnormality of heart dysfunction in type 2 diabetes (by formation of peroxynitrite due to iNOS activation) and development of hypertension and insulin resistance in metabolic syndrome. (ubc.ca)
  • iNOS is calciumindependent, expressed by many cell types (especially mononuclear phagocytes, hepatocytes, chondrocytes and smooth muscle cells) and is responsible for high output NO production (1-3). (edu.au)
  • He is exploring the vasoconstrictory role of a well-known growth receptor (epidermal growth factor receptor) pathway and the vasodilatory inducible nitric oxide synthase (iNOS) pathway in normal and diabetic arteries. (msfhr.org)
  • NO is produced by Nitric Oxide Synthase (NOS) which exist in three isoforms, inducible NOS (iNOS), endothelial NOS (ecNOS) and neuronal NOS (nNOS). (omicsonline.org)
  • Mammalian inducible nitric oxide synthase (iNOS) catalyzes the production of L-citrulline and nitric oxide (NO) from L-arginine and O_2. (caltech.edu)
  • Using a quantitative, competitive RT-PCR technique, we recently showed that expression of interferon γ (IFN-γ) and IFN-γ inducible type of nitric oxide synthase (iNOS) is increased in homogenised liver tissue of patients with chronic HCV infection. (bmj.com)
  • IFN-γ has been shown to upregulate the inducible isoform of nitric oxide synthase (iNOS) in monocytes/macrophages, resulting in NO production. (bmj.com)
  • Abstract In the kidney, nitric oxide (NO) from the macula densa (MD) is considered an integral modulator of the tubulovascular message system, whereas endothelium-derived NO is a major vasorelaxing factor. (ahajournals.org)
  • This study investigated whether type II nitric oxide synthase (NOS2) expression correlated with cell proliferation in oral squamous cell carcinoma (OSCC) and dysplasia. (nih.gov)
  • Type 2 nitric oxide synthase (NOS2) is required for the Th1-dependent healing of infections with intracellular microbes, including Leishmania major. (nih.gov)
  • To investigate the effects of loss of nitric oxide synthase type II (NOS2) in the healing of an injured corneal stroma in mice. (arvojournals.org)
  • Ocular fibroblasts from wild type (WT) and KO mice were used to study the role of NOS2 on fibrogenic gene expression. (arvojournals.org)
  • Nitric oxide synthase, inducible is an enzyme which is encoded by the NOS2 gene in humans and mice. (wikipedia.org)
  • They lack the gene encoding nitric oxide synthase 2 (Nos2) and are susceptible to murine CMV infection. (wikipedia.org)
  • MRL-Ipr/lpr mice overexpress inducible nitric oxide synthase (NOS2) and overproduce NO in parallel with the development of an autoimmune syndrome with a variety of inflammatory manifestations . (musc.edu)
  • MRL-lpr/Ipr littermates homozygous for disrupted NOS2 (-/-) , heterozygous for disrupted NOS2 (+/-) , or wild- type (+/+) were derived for this study . (musc.edu)
  • NOS2 (Nitric Oxide Synthase 2) is a Protein Coding gene. (genecards.org)
  • In the instant invention, rho GTPase function inhibitors are found to upregulate endothelial cell Nitric Oxide Synthase activity. (google.ca)
  • As a result, rho GTPase function inhibitors are useful in treating or preventing conditions that result from the abnormally low expression and/or activity of endothelial cell Nitric Oxide Synthase. (google.ca)
  • In the instant invention, HMG-CoA reductase inhibitors are found to upregulate endothelial cell Nitric Oxide Synthase activity through a mechanism other than preventing the formation of oxidative-LDL. (google.com)
  • Selective inhibitors of neuronal nitric oxide synthase (nNOS) are regarded as valuable and powerful agents with therapeutic potential for the treatment of chronic neurodegenerative pathologies and human melanoma. (rcsb.org)
  • However, mice deficient in neuronal nitric oxide synthase have a similar susceptibility to volatile anaesthetic agents to wild-type mice and are not affected by non-isoform selective inhibitors. (elsevier.com)
  • Nitric oxide inhibitors make antibiotics work more effectively. (asbmb.org)
  • Thomas Poulos' group at the University of California, Irvine , have been collaborating with Richard Silverman 's group at Northwestern University to develop inhibitors for a protein called nitric oxide synthase , or NOS. (asbmb.org)
  • The possibility that nitric oxide synthase (NOS) inhibitors influence dynorphin immunoreactivity following hyperthermia was examined in a rat model using a pharmacological approach. (lu.se)
  • Neuroprotective effects of nitric oxide synthase inhibitors in spinal cord injury-induced pathophysiology and motor functions: An experimental study in the rat. (lu.se)
  • Nitric Oxide Synthase (NO synthase) Inhibitors - Pipeline Insights, 2017" provides in depth insights on the pipeline drugs and their development activities around the Nitric Oxide Synthase (NO synthase) Inhibitors. (researchandmarkets.com)
  • This report also provides detailed information on the discontinued and dormant drugs that have gone inactive over the years for Nitric Oxide Synthase (NO synthase) Inhibitors. (researchandmarkets.com)
  • This report also assesses the Nitric Oxide Synthase (NO synthase) Inhibitors therapeutics by Monotherapy, Combination products, Molecule type and Route of Administration. (researchandmarkets.com)
  • The ability of IFN-gamma to inhibit replication of ectromelia, vaccinia, and herpes simplex-1 viruses in mouse macrophages correlated with the cells' production of nitric oxide (NO). Viral replication was restored in IFN-gamma-treated macrophages exposed to inhibitors of NO synthase. (sciencemag.org)
  • We studied in a large sample (763 subjects, age range 19-107†years) the variability of the three human genes (NOS1, -2, -3) coding for the three isoforms of the NADPH-dependent enzymes named NO synthases (NOS) which are responsible of NO synthesis. (isharonline.org)
  • In mammals, NO synthesis is performed by three isoforms (inducible, endothelial, and neuronal) of homodimeric nitric-oxide synthases (NOS, EC 1.14.13.39). (pubmedcentralcanada.ca)
  • Exposures to 2.0 and 2.8 ATA O(2) stimulated neuronal (type I) NO synthase (nNOS) and significantly increased steady-state.NO concentration, but the mechanism for enzyme activation differed at each partial pressure. (isharonline.org)
  • Gene polymorphisms of endothelial constitutive nitric oxide synthase (ecNOS), angiotensin converting enzyme (ACE) and angiotensin II type 1 receptor (AT1R) have been found to be associated with atherosclerosis. (medeniyet.edu.tr)
  • Nitroindazole acts as a selective inhibitor for neuronal nitric oxide synthase , a hemoprotein enzyme that, in neuronal tissue, converts arginine to citrulline and nitric oxide (NO). [1] Nitric oxide can diffuse through the plasma membrane into neighbouring cells, allowing cell signalling , so nitroindazole indirectly inhibits this signalling process. (wikipedia.org)
  • Calcium/calmodulin-dependent protein kinase type IV is an enzyme that in humans is encoded by the CAMK4 gene. (wikipedia.org)
  • The purpose of this study was to examine cardiovascular responses to fourth cerebral ventricle (4V) administration of nitroglycerin (NTG) or an inhibitor of nitric oxide (NO) synthase (NOS) in the near-term ovine and to determine whether, during birth, neuronal NOS (nNOS) is induced in noradrenergic A1 neurons in the medial nucleus tractus solitarius (mNTS). (isharonline.org)
  • Without syntrophin, utrophin and neuronal nitric oxide synthase μ (nNOSμ) fail to localize to the NMJ and the AChRs are distributed abnormally. (jneurosci.org)
  • Its synthesis in neurons has been most commonly ascribed to the neuronal nitric oxide synthase (nNOS) isoform. (frontiersin.org)
  • Nitric oxide (NO) produced by neuronal NO synthase (nNOS) in the macula densa decreases tubuloglomerular feedback (TGF). (ahajournals.org)
  • Nitric oxide (NO) synthesized by neuronal NO synthase (nNOS) in the macula densa is an important modulator of TGF. (ahajournals.org)
  • Here we describe that, in hippocampal pyramidal cells, neuronal nitric oxide synthase (nNOS) is also associated with the postsynaptic active zones of GABAergic symmetrical synapses terminating on their somata, dendrites, and axon initial segments in both mice and rats. (jneurosci.org)
  • Our results demonstrate that NOsGC, composed of α 1 β 1 subunits, is selectively expressed in different types of interneurons and is present in their presynaptic GABAergic terminals, in which it may serve as a receptor for NO produced postsynaptically by nNOS in the very same synapse. (jneurosci.org)
  • As described in Chapter 42, niric oxide (NO) is synthesized from the amino acid l-arginine by the actions of a family of enymes, the NO synthases (NOS), each isoform of which is encoded by a separate gene. (edu.au)
  • Wang H, Kohr M, Traynham C, Ziolo M. Phosphodiesterase 5 restricts NOS3/Soluble guanylate cyclase signaling to L-type Ca2+ current in cardiac myocytes. (labome.org)
  • administering to a subject in need of such treatment a rho GTPase function inhibitor in an amount effective to increase endothelial cell Nitric Oxide Synthase activity in said tissue of the subject, provided that the rho GTPase function inhibitor is not an agent selected from the group consisting of a HMG-CoA reductase inhibitor, a protein kinase C inhibitor, a tyrosine kinase inhibitor, and cyclosporin. (google.ca)
  • Doshi A, Ziolo M, Wang H, Burke E, Lesinski A, Binkley P. A promoter polymorphism of the endothelial nitric oxide synthase gene is associated with reduced mRNA and protein expression in failing human myocardium. (labome.org)
  • This report summarizes some of the current information regarding NO synthase structure-function, reaction mechanism, control of catalysis, and protein interactions. (labome.org)
  • In this regard, AngII stimulates hypertrophy, proliferation, and migration of vascular smooth muscle cells (VSMCs) through a G protein-coupled receptor (GPCR), the AngII type-1 receptor (AT 1 ). (ahajournals.org)
  • AMPK and Endothelial Nitric Oxide Synthase Signaling Regulates K-Ras Plasma Membrane Interactions via Cyclic GMP-Dependent Protein Kinase 2. (uchicago.edu)
  • Inducible nitric oxide synthase (i NOS ) has previously been shown to contribute to atherosclerotic lesion formation and protein nitration . (rsc.org)
  • p>This indicates the type of evidence that supports the existence of the protein. (uniprot.org)
  • Before and during treatment nitric oxide synthase activity and inducible nitric oxide synthase protein, cGMP, nitrate plus nitrite and interleukin 8 (IL-8) levels were measured in urine. (greenmedinfo.com)
  • ET-1 mRNA expression was increased in the inner medulla of the kidney , whereas protein expression of ET receptor type B (ET(B)R) was unchanged. (bvsalud.org)
  • We used immunohistochemical techniques and antibodies directed against inducible nitric oxide synthase, glial fibrillary acidic protein, and vimentin. (kuleuven.be)
  • Based on morphology and on glial fibrillary acidic protein and vimentin immunoreactivity, this inducible nitric oxide synthase immunoreactivity appeared to localize to retinal Müller glial cells. (kuleuven.be)
  • A unique phosphorylation-dependent mechanism for the activation of Ca2+/calmodulin-dependent protein kinase type IV/GR". J. Biol. (wikipedia.org)
  • Endothelial nitric oxide synthetase gene polymorphism was evaluated in the case and control groups. (ac.ir)
  • It also causes more nitric oxide secretion from vascular endothelial cells and opening of potassium channels. (magiran.com)
  • it depends on fat depots (visceral or subcutaneous), the cell type composition (mature adipocytes, stromal-vascular cells, and nonfat cells including macrophages), and so on. (hindawi.com)
  • Nitric oxide originating from the endothelial cells of the vessel wall is essential for the vascular system. (garvan.org.au)
  • Among the most important cardiovascular actions of insulin is to stimulate production of the potent vasodilator nitric oxide (NO) from vascular endothelium, increasing blood flow to skeletal muscle and other target tissues. (intechopen.com)
  • Abnormally high insulin concentrations (i.e. early stage of type 2 diabetes) and/or insulin resistance (i.e. tissues do not respond to insulin normally) have a profound impact on vascular homeostasis that manifests as impaired endothelial function, vasodilation, microvessel disease (i.e. retinopathy and nephropathy), and enhanced vascular inflammation and atherosclerotic lesion formation. (intechopen.com)
  • PURPOSE: Inducible nitric oxide synthase has been implicated in the pathogenesis of cerebral ischemic damage, in the angiogenic process and in diabetic vascular damage. (kuleuven.be)
  • The latest report Nitric Oxide Synthase Brain - Pipeline Review, H1 2017, outlays comprehensive information on the Nitric Oxide Synthase Brain (Constitutive NOS or NC NOS or Neuronal NOS or bNOS or NOS Type I or Peptidyl Cysteine S Nitrosylase NOS1 or NOS1 or EC 1.14.13.39) targeted therapeutics, complete with analysis by indications, stage of development, mechanism of action (MoA), route of administration (RoA) and molecule type. (researchmoz.us)
  • It also reviews key players involved in Nitric Oxide Synthase Brain (Constitutive NOS or NC NOS or Neuronal NOS or bNOS or NOS Type I or Peptidyl Cysteine S Nitrosylase NOS1 or NOS1 or EC 1.14.13.39) targeted therapeutics development with respective active and dormant or discontinued projects. (researchmoz.us)
  • The aim of the study was to verify the hypothesis that intoxication with low doses of mycotoxins leads to changes in the m RNA expression levels of nitric oxide synthase-1 and nitric oxide synthase-2 genes in tissues of the gastrointestinal tract and the liver. (mdpi.com)
  • The lowest m RNA expression levels of nitric oxide synthase-1 and nitric oxide synthase-2 genes were noted in the sixth week of the study, in particular in group M. Our results suggest that the presence of low mycotoxin doses in feed slows down the m RNA expression of both nitric oxide synthase isomers, which probably lowers the concentrations of nitric oxide, a common precursor of inflammation. (mdpi.com)
  • The expression of endothelin 1 (ET-1) and nitric oxide synthase (NOS) genes in the heart and lung of broiler chickens has been affected by feeding Urtica dioica to broiler chickens. (magiran.com)
  • Relationships between inducing genes of nitric oxide synthase and apoptosis in the inflammatory gingival tissue. (nii.ac.jp)
  • Insulin resistance, hyperlipidemia, and hypertension in mice lacking endothelial nitric oxide synthase. (semanticscholar.org)
  • Impaired Cognitive Performance in Endothelial Nitric Oxide Synthase Knockout Mice After Ischemic Stroke: A Pilot Study. (uchicago.edu)
  • Neuroprotection Mediated by Upregulation of Endothelial Nitric Oxide Synthase in Rho-Associated, Coiled-Coil-Containing Kinase 2 Deficient Mice. (uchicago.edu)
  • Exposure of mice to lipopolysaccharide (LPS) plus interferon-gamma (IFN-gamma) increases nitric oxide (NO) production, which is proposed to play a role in the resulting pulmonary damage and inflammation. (cdc.gov)
  • We hypothesized that the neuronal nitric oxide synthase selective inhibitor, 7-nitroindazole, would also reduce the propofol requirements in wild-type mice but would have no effect in neuronal nitric oxide synthase knockout mice. (elsevier.com)
  • Methods: We determined the time to loss of righting reflex, time to painful stimulus and time to regaining the righting reflex in neuronal nitric oxide synthase knockout and wild-type mice following the intraperitoneal injection of propofol in untreated, 7-nitroindazole and vehicle only treated animals (n = 6 per group). (elsevier.com)
  • 0.001) in both wild-type and knockout mice when compared to untreated and vehicle only pre-treated animals, but had no effect on time to painful stimulus or regaining of the righting reflex. (elsevier.com)
  • 7-nitroindazole reduced the propofol requirements in knockout mice to the same extent as in wild-type animals. (elsevier.com)
  • Salmonella deficient in Cu,Zn-SOD has reduced survival in macrophages and attenuated virulence in mice, which can be restored by abrogation of either the phagocyte respiratory burst or inducible nitric oxide synthase. (pnas.org)
  • Clinical and serologic manifestations of autoimmune disease in MRL-lpr/lpr mice lacking nitric. (musc.edu)
  • In previous studies , we showed that inhibiting NO production with the nonselective nitric oxide inhibitor N -monomethyl-arginine reduced glomerulonephritis , arthritis , and synthase (NOS) c vasculitis in MRL-lpr/lpr mice . (musc.edu)
  • Furthermore, both ex vivo and in vivo studies demonstrated that inducible nitric oxide synthase induction in the peritoneal cavity and brain is unimpaired in receptor-deficient mice. (jimmunol.org)
  • Coronary hemodynamics in endothelial NO synthase knockout mice. (semanticscholar.org)
  • Conversely, epithelial cells with no detectable NO synthesis restricted viral replication when transfected with a complementary DNA encoding inducible NO synthase or treated with organic compounds that generate NO. In mice, an inhibitor of NO synthase converted resolving ectromelia virus infection into fulminant mousepox. (sciencemag.org)
  • To identify the lung cell type expressing the NOS-II mRNA, specific 473 base pair DIG-labelled cDNA probe was hybridized in situ in sections of rat lung. (polaros.com)
  • Background: Due to the pleiotropic effects of nitric oxide (NO) within the lungs, it is likely that NO is a significant factor in the pathogenesis of chronic obstructive pulmonary disease (COPD). (uib.no)
  • Does type II nitric oxide synthase expression correlate with cellular proliferation in oral squamous cell carcinoma and dysplasia? (nih.gov)
  • Sullivan J, Smart E, Pollock D, Pollock J. Influence of salt on subcellular localization of nitric oxide synthase activity and expression in the renal inner medulla. (labome.org)
  • Inhibitory effect of IGF-I on type 2 nitric oxide synthase expression in Ins-1 cells and protection against activation-dependent apoptosis: involvement of phosphatidylinositol 3-kinase. (diabetesjournals.org)
  • Challenge of Ins-1 cells, a rat beta-pancreatic cell line, with lipopolysaccharide (LPS) and interferon-gamma (IFN-gamma) promoted the expression of type 2 nitric oxide synthase (NOS-2) in a cooperative way. (diabetesjournals.org)
  • The present study was to examine the distribution of transient receptor potential vanilloid type-1 (TRPV1) receptor immunoreactivity in the acupuncture points (acupoint), and determine the influences of electroacupuncture (EA) stimulation on TRPV1 expression. (isharonline.org)
  • Analysis of mononuclear cell nitric oxide synthase type 2 antigen expression. (duke.edu)
  • Here we determined whether oxidative stress induced by the spinal activation of nitric oxide synthase type II (NOS-II) modulates the expression of P450c17 and whether this process contributes to the development of neuropathic pain in rats. (bvsalud.org)
  • Nitric Oxide Synthase in human breast cancer is associated with tumor grade, proliferation rate and expression of progesterone receptors. (thefreedictionary.com)
  • Background- In the arterial endothelium, laminar flow and cyclical stretch induce expression of NO synthase (NOS). (ahajournals.org)
  • The levels of nitric oxide synthase (NOS), Endothelin 1 (ET-1) and β-actin transcripts were determined by real-time reverse transcriptase (RT)-PCR. (magiran.com)
  • In vitro studies with peritoneal macrophages stimulated with IFN-γ and tachyzoites indicated that under limiting concentrations of IFN-γ, nitric oxide-mediated toxoplasmastatic activity is TNF-α dependent. (jimmunol.org)
  • Background and objective: The glutamate-nitric oxide-cyclic guanosine monophosphate pathway has been identified as a potential target for anaesthetic agents. (elsevier.com)
  • Conclusions: Propofol exerts its anaesthetic effects at least partially via the glutamate-nitric oxide-cyclic guanosine monophosphate pathway. (elsevier.com)
  • PURPOSE: We attempted to determine whether oral L-arginine, the substrate for nitric oxide synthase, increases nitric oxide synthase activity and cyclic guanosine monophosphate (cGMP) levels in the urine from interstitial cystitis patients. (greenmedinfo.com)
  • The NO receptor nitric oxide-sensitive guanylyl cyclase (NOsGC) is present in the brain in two functional subunit compositions: α 1 β 1 and α 2 β 1 . (jneurosci.org)
  • We here evaluated the association of serum levels of glycated albumin (GA) and endogenous secretory receptor for advanced glycation end products (esRAGE) with coronary artery remodeling in type 2 diabetic patients. (springer.com)
  • On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. (uniprot.org)
  • The lack of TNF receptors did not adversely affect the development of a type 1 IFN-γ response. (jimmunol.org)
  • Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. (uniprot.org)
  • Each monomer is comprised of a nitric-oxide synthase oxygenase domain (NOSoxy), 3 with structurally defined binding sites for its substrate l -arginine, tetrahydrobiopterin (H 4 B), and a Cys-coordinated Fe-protoporphyrin IX moiety (heme), and a C-terminal reductase domain (NOSred) with binding sites for NADPH, FAD, and FMN. (pubmedcentralcanada.ca)
  • Acute amelioration of diabetic endothelial dysfunction with a derivative of the nitric oxide synthase cofactor, tetrahydrobiopterin. (thefreedictionary.com)
  • SA Abd El-Aleem, Abdelwahab S, Osman NMS (2017) Co-upregulation of Transforming Growth Factor Beta-1 and Nitric Oxide Synthase in Keloid by Comparison to Normal Human Skin - A Possible Role for TGFβ1 and NOS in Pathogenesis of Keloid. (omicsonline.org)
  • The purpose of these studies was to determine the role of gracile nucleus and the effects of l-arginine-derived nitric oxide (NO) synthesis in the nucleus on the cardiovascular responses to electroacupuncture (EA) stimulation of 'Zusanli' (ST36). (isharonline.org)
  • 2 L-arginine + 3 NADPH + 4 O 2 = 2 L-citrulline + 2 nitric oxide + 3 NADP + + 4 H 2 O. (uniprot.org)
  • Effect of long-term oral L-arginine on the nitric oxide synthase pathway in the urine from patients with interstitial cystitis. (greenmedinfo.com)
  • Nitric oxide synthases (NOS) catalyze the production of NO by the five-electron oxidation of a guanidino nitrogen atom of the substrate L-Arginine which requires NADPH FAD FMN heme and O2as cofactors (10). (exposed-skin-care.net)
  • This entry was posted in Lung injury and tagged 1-Amino-2-hydroxy-guanidine , Endotoxic shock , NG-nitro-L-arginine , Nitric oxide , Nitric oxide synthase type II , Nitric oxide synthase type III , S-nitroso-N-acetyl-penicillamine . (polaros.com)
  • The nitric oxide synthase oxygenase domain (NOS_(ox)) oxidizes arginine to synthesize the cellular signal and defensive cytotoxin nitric oxide (NO). Crystal structures determined for cytokine-inducible NOS_(ox) reveal an unusual fold and heme environment for stabilization of activated oxygen intermediates key for catalysis. (caltech.edu)
  • Protective effects of L-arginine were evaluated in a human ventricular heart cell model of low-volume anoxia and reoxygenation independent of alternate cell types. (biomedsearch.com)
  • CONCLUSIONS: These observations suggest that Müller cells may be involved in the microvascular remodeling of the diseased retina and that high concentrations of nitric oxide produced by inducible nitric oxide synthase could contribute to neurotoxicity and angiogenesis that occur in diabetic retinopathy. (kuleuven.be)
  • Oxidative stress is also a crucial component in obesity, sympathetic activation and metabolic disorders such as hypertension, dyslipidaemia and type 2 diabetes/insulin resistance, which aggregate with OSAHS. (ersjournals.com)
  • 1-3 Importantly, in addition to vasoconstriction induced by AngII, other pathological effects of AngII such as induction of growth and migration are blocked by nitric oxide (NO) in vitro. (ahajournals.org)
  • Induction of the inducible nitric-oxide synthase gene by intratracheal instillation of silica, coal, titanium-dioxide and carbonyl iron. (cdc.gov)
  • Nitric oxide (NO) and reactive oxygen species are two key components in the induction of the hypersensitive response (HR) during plant defense against pathogen infection. (openthesis.org)
  • Thus, induction of NO synthase can be necessary and sufficient for a substantial antiviral effect of IFN-gamma. (sciencemag.org)
  • Endothelial dysfunction plays a central role in atherosclerotic progression and cardiovascular complications of type 2 diabetes mellitus (T2DM). (biomedcentral.com)
  • Activation of RAGE is a significant pathogenetic mechanism for the development of cardiac dysfunction in type 2 diabetes. (wiley.com)
  • The role of nitric oxide (NO) in spinal cord injury (SCI)-induced motor dysfunction, breakdown of the blood-spinal cord barrier (BSCB), edema formation, and cell injury was examined using a pharmacological approach. (lu.se)
  • Folic acid prevents nitroglycerin-induced nitric oxide synthase dysfunction and nitrate tolerance: a human in vivo study. (thefreedictionary.com)
  • In contrast, in the cortical vasculature selectively enhanced nitrotyrosine immunoreactivity was detected in the arteriolar wall of the nonclipped contralateral kidney, and endothelial NADPH-d signal, indicating NOS Type III activity, was enhanced in parallel. (ahajournals.org)
  • RESULTS: Immunoreactivity for inducible nitric oxide synthase was not observed in retinas from all subjects without diabetes and without ocular disease. (kuleuven.be)
  • Six retinas from three subjects with diabetes and nonproliferative retinopathy, and the retina from the eye with ocular ischemic syndrome showed immunoreactivity for inducible nitric oxide synthase in cells with elongated processes. (kuleuven.be)
  • Nitric oxide and cGMP are decreased in urine from interstitial cystitis patients and both induce smooth muscle relaxation and immunological responses. (greenmedinfo.com)
  • Increasing urinary nitric oxide and cGMP may ameliorate interstitial cystitis symptoms. (greenmedinfo.com)
  • Nitric oxide (NO) production, NO synthase (NOS) activity, cGMP levels, and cellular injury were assessed. (biomedsearch.com)
  • Genetic factors play important role in the development of type 2 diabetes and diabetic nephropathy. (cdc.gov)
  • Patients and Methods: This study conducted on 45 patients with diabetes mellitus type 2 and 15 healthy subjects serving as a control group. (journalcra.com)
  • Hans-Henrik Parving, Gentofte, Denmark, pointed out that kidney disease develops in 40% of patients with type 2 diabetes, with 25% of patients in Europe and 46% in the U.S. with end-stage renal disease (ESRD) having diabetes. (diabetesjournals.org)
  • In type 2 diabetes, microalbuminuria is associated with a 5-10% lifetime risk of progression to overt nephropathy, while patients with normoalbuminuria have a 10- to 20-fold lower risk. (diabetesjournals.org)
  • Barry Brenner, Boston, MA, discussed the Reduction of Endpoints in NIDDM with the Angiotensin II Antagonist Losartan (RENAAL) trial of 1,513 patients with type 2 diabetes and nephropathy from 250 centers in 29 countries. (diabetesjournals.org)
  • Approximately 90-95% of patients with diabetes mellitus have type 2 diabetes, which is characterized by insulin resistance or the inability of insulin to exert its metabolic actions. (intechopen.com)
  • This study was undertaken to determine whether inducible nitric oxide synthase is present in the retinas from human subjects with diabetes mellitus. (kuleuven.be)
  • Compared to wild-type non-diabetic subjects, all patients with T2DM had a significantly increased risk of AMI regardless of genotype. (biomedcentral.com)
  • T) in 159 type 2 diabetic patients without macrovascular complications and in 207 healthy control subjects. (semanticscholar.org)
  • A total of 200 type 2 diabetic patients and 100 apparently healthy volunteers as controls were included in the study. (cdc.gov)
  • Using MRI and conductance catheter techniques, we evaluated cardiac function in a type 2 diabetic mouse model (db/db), and assessed the effect of blocking RAGE with a RAGE antibody. (wiley.com)
  • Background: Nephropathy is the most important leading cause of end stage renal failure in type 2 diabetic patients, so numerous studies were done to diagnose and evaluate risk factors of diabetic nephropathy (DN). (ac.ir)
  • Materials and Methods: In a cross-sectional study, 94 type 2 diabetic patients and 94 normal participants were enrolled. (ac.ir)
  • What was not available in 1993 was "hard end point data" in type 2 diabetic subjects with nephropathy that showed losartan could slow the progression of advancing renal disease to nephropathy. (diabetesjournals.org)
  • Gene transfer of recombinant endothelial nitric oxide synthase to liver in vivo and in vitro. (labome.org)
  • Our results demonstrate that in normothermic resting humans in vivo, activation of PAR2 elicits cutaneous vasodilatation partly through nitric oxide synthase-dependent mechanisms, but does not mediate sweating. (nih.gov)
  • These observations suggest that SOD protects periplasmic or inner membrane targets by diverting superoxide and limiting peroxynitrite formation, and they demonstrate the ability of the respiratory burst and nitric oxide synthase to synergistically kill microbial pathogens in vivo . (pnas.org)
  • Nitric oxide (NO) is known to act as a mediator of tissue injury as well as being a potent endogenous vasodilator. (unboundmedicine.com)
  • Nitric oxide is known to be an important inflammatory mediator, and is implicated in the pathophysiology of a range of inflammatory disorders. (mysciencework.com)
  • Nitric oxide (NO) is an important mediator of many pro-growth signaling cascades in cancer (6-9). (exposed-skin-care.net)
  • Nitric oxide (NO) is an important mediator of the inflammatory response . (musc.edu)
  • Nitric oxide is an important mediator in cell-to-cell communication. (ahajournals.org)
  • Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. (genecards.org)
  • Nitric oxide as a mediator of relaxation of the corpus cavernosum in response to nonadrenergic, noncholinergic neurotransmission. (nature.com)
  • 3. The method of claim 1 wherein the amount is sufficient to increase endothelial cell Nitric Oxide Synthase activity above normal baseline levels. (google.ca)
  • 4. The method of claim 1 wherein the subject has a condition comprising an abnormally low level of endothelial cell Nitric Oxide Synthase activity which is chemically induced. (google.ca)
  • 12. The method of claims 1 - 9 , further comprising co-administering at least one different rho GTPase function inhibitor in an amount effective to increase endothelial cell Nitric Oxide Synthase activity in said tissue of the subject. (google.ca)
  • 14. The method of claims 1 - 9 , further comprising co-administering a non-rho GTPase function inhibitor agent that increases endothelial cell Nitric Oxide Synthase activity. (google.ca)
  • administering to a nonhypercholesterolemic subject in need of such treatment a HMG-CoA reductase inhibitor in an amount effective to increase endothelial cell Nitric Oxide Synthase activity in said tissue of the subject. (google.com)
  • Positive staining of the EGM was paralleled by enhanced NADPH diaphorase (NADPH-d) staining of the adjacent MD, signifying increased type I nitric oxide synthase (NOS) activity in the stenotic kidney. (ahajournals.org)
  • Dail WG, Barba V, Leyba L, Galindo R . Neural and endothelial nitric oxide synthase activity in rat penile erectile tissue. (nature.com)
  • Interferons (IFNs) induce antiviral activity in many cell types. (sciencemag.org)