Nih 3t3 cells does significantly. Medical search. Frequent questions

Extraction from 9 parameters of KSO-SFE was screened for cytotoxicity towards human colorectal cancer cell lines (HT29) and mouse embryonic fibroblast (NIH/3T3) cell lines using MTS assay. (hindawi.com)
Human colorectal cancer (HT29) and mouse embryonic fibroblast (NIH/3T3) cell lines were purchased from the American Type Culture Collection (ATCC), USA. (hindawi.com)
Three cell linesâ MG-63 osteosarcoma, HeLa cervical cancer, and fibroblast NIH/3T3 were examined. (bvsalud.org)
Finally, the in vitro antiproliferative activity of all the complexes was evaluated against human cervical cancer (HeLa) and mouse embryonic fibroblast (NIH-3T3) cell lines by MTT assay and the results suggested that the complexes were significantly active compared to various clinically reported drugs. (uniss.it)
This study aimed to analyze and compare the effect of a micro nanoencapsulated of the porphyrin 3MMe in NIH 3T3 fibroblast lines, B16-F10 murine melanoma and melanocytes lines Melan-A in vitro through indicators of cell viability and function. (unip.br)
To analyze the protective effects against complement-mediated cytolysis of the MCP , DAF , and CD59 human complement regulatory proteins, alone and in combination, on NIH 3T3 mouse fibroblast cells. (ectrx.org)
MTT assay was performed to determine the cytotoxic effects of the compounds on A549 human lung adenocarcinoma, HepG2 human hepatocellular carcinoma, C6 rat glioma, and NIH/3T3 mouse embryonic fibroblast cell lines. (anadolu.edu.tr)

The levels and subcellular distribution of the interferon-inducible double-stranded RNA-dependent protein kinase PKR have been measured in human Daudi cells and stably transfected mouse NIH 3T3 cells expressing the human protein kinase. (pasteur.fr)
Viability of NIH 3T3 cells stably transfected with these plasmids was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. (ectrx.org)

It delivers exceptional transfection efficiency with improved cell viability for the widest range of hard-to-transfect and common cell types including HEK 293, HeLa, LNCaP, HepG2, and A549 cell lines (Figure 1) . (thermofisher.com)
Each reagent was used to transfect HEK 293, HeLa, LNCaP, HepG2, and A549 cell lines in a 96-well format, and GFP expression was analyzed 48 hours post-transfection. (thermofisher.com)
Each reagent was used to transfect HeLa cells in a 96-well format at the indicated doses with an emerald green fluorescent protein (GFP)-expressing vector. (thermofisher.com)
Bafilomycin A1 inhibits the growth of a variety of cultured cells dose-dependently, including golden hamster embryo, NIH-3T3 fibroblasts, PC12 and HeLa cells. (targetmol.com)

The compounds were evaluated for their in vitro antimicrobial effects on pathogenic bacteria and Candida species using microdilution and ATP luminescence microbial cell viability assays. (anadolu.edu.tr)

RESEARCH DESIGN AND METHODS- We examined Lcn2 expression in murine tissues and in 3T3-L1 adipocytes in the presence and absence of various stimuli. (diabetesjournals.org)
To assess effects on insulin action, we used retroviral delivery of short hairpin RNA to reduce Lcn2 levels in 3T3-L1 adipocytes. (diabetesjournals.org)
Lcn2 serum levels are elevated in multiple rodent models of obesity, and forced reduction of Lcn2 in 3T3-L1 adipocytes improves insulin action. (diabetesjournals.org)
Further, ADP-1 beneficially affected lipid metabolism by enhancing lipid globule formation in mouse 3T3-L1 adipocytes. (phoenixpeptide.com)
We investigated the role of S100B in the interaction between adipocytes and macrophages using a cell-culture model. (nih.gov)
3T3-L1 adipocytes (L1) were stimulated by TNF-α to examine S100B secretion. (nih.gov)

Cell cycle analysis showed a significant increase in the accumulation of KSO-SFE-treated cells at sub-G1 phase, indicating the induction of apoptosis by KSO-SFE. (hindawi.com)
A feeding ratio of PEDOT-PC exceeding 70% resulted in cell apoptosis. (bvsalud.org)
In addition to inducing necrosis and apoptosis, ROS induces autophagic cell death. (biomedcentral.com)
Treating SCCHN cell lines with a pan-Aurora kinase inhibitor resulted in defective cytokinesis, polyploidy and apoptosis, which was effective irrespective of the EGFR status. (oncotarget.com)
Our results demonstrate that combined targeting of EGFR and Aurora kinases represents a therapeutic means to activate cell cycle checkpoints and apoptosis in SCCHN. (oncotarget.com)
It is also found to inhibit autophagy while induces apoptosis.Bafilomycin A1 triggers proliferative potential of senescent cancer cells in vitro and in NOD/SCID mice. (targetmol.com)
Rigosertib was more potent than wortmannin and rapamycin in inducing sub-G1 and G2-M cell cycle arrest, as well as late apoptosis in the Inf-3T3 and MT-2 cells. (htlv.net)

Eplin-α mRNA is detected in various tissues and cell lines, but strikingly absent or downregulated in cancer cells [ 2 ]. (biomedcentral.com)
Lipofuscin consists of highly autofluorescent granules of oxidized proteins and lipids that build up in the lysosomes of aging cells in a variety of tissues. (biotium.com)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
While many studies have demonstrated the importance of cTEC and mTEC during T-cell development, thymic stromal microenvironments also contain heterogenous non-epithelial stromal (NES) populations in a similar manner to that seen in peripheral lymphoid tissues. (frontiersin.org)
genic models are inadequate for number of activated CD8-positive T LMP1 was strongly expressed in the understanding the cancer etiology in cells increased considerably in the lymphoma tissues but was hardly the context of natural viral infection. (who.int)

Previous study has shown that kenaf seed oil extracted by SFE (KSO-SFE) is cytotoxic towards human cervical cancer cell line [ 7 ]. (hindawi.com)

These cell types can be classified as a) cells that are physiologically under high mechanical tension (e.g., smooth muscle in the wall of hollow organs, epithelial and endothelial cells), b) cells that have high rates of proliferation (e.g., myoblasts), and c) cells that are actively migrating (e.g., fibroblasts and macrophages). (wikipedia.org)
In this study, differences in choline membrane metabolism were characterized in breast cancer cells and normal human mammary epithelial cells (HMECs) labeled with [1,2- 13 C]choline, using 1 H and 13 C magnetic resonance spectroscopy. (aacrjournals.org)
Epithelial Protein Lost in Neoplasm α is a novel cytoskeleton-associated tumor suppressor whose expression inversely correlates with cell growth, motility, invasion and cancer mortality. (biomedcentral.com)
In epithelial cells, Eplin is required for formation of the F-actin adhesion belt by binding to the E-cadherin-catenin complex through α-catenin [ 6 ]. (biomedcentral.com)
Extensive research on thymic epithelial cells (TEC) within the cortex and medulla has defined their essential roles during T-cell development. (frontiersin.org)
Significantly, there are additional non-epithelial stromal cells (NES) that exist alongside TEC within thymic microenvironments, including multiple subsets of mesenchymal and endothelial cells. (frontiersin.org)
Arguably, the most recognized and well-studied stromal populations in thymus biology are thymic epithelial cells (TEC) within the cortical and medullary areas, which are defined by their anatomical separation and function. (frontiersin.org)
Cortical epithelial cells (cTEC) support the earliest thymocyte progenitor populations, guiding them through the cortex, directing them to a T-cell lineage fate and ensuring their functional qualities as self-MHC restricted cells through the process of positive selection ( 2 ). (frontiersin.org)
Medullary epithelial cells (mTEC), in conjunction with dendritic cells, then take over control of developing thymocytes. (frontiersin.org)
Broadly separated into mesenchymal and endothelial cells, NES have been implicated in thymus organogenesis, thymocyte development, tolerance induction and development/maintenance of epithelial stroma. (frontiersin.org)
In this review, we cover how the non-epithelial compartment of thymic stroma represent essential cell populations in regulating thymus function. (frontiersin.org)

This endogenous current can be significantly reduced by antisense oligonucleotides against I Cln , indicating that I Cln is the prevalent protein involved in I Cl ( 5 ). (biomedcentral.com)
The best behaved probe showed no dependence on the concentration of common endogenous cations, selected reductants and serum albumin and was examined in live cell imaging studies and used to monitor time‐dependent lysosomal acidification, for which the increase in observed image brightness due to acidification was found to be a factor of 50 in NIH‐3T3 cells. (worktribe.com)
Nevertheless, in HEK 293T cells, we could not co precipitate the endogenous TAK1 and S6K1. (phosphorylaseinhibitors.com)
We tested the endogenous interaction in NIH 3T3 cells, but we couldnt observe the immune complex, either. (phosphorylaseinhibitors.com)

A hypothesis is that higher level of calponin 2 is required in fast proliferating cells to maintain the dynamic equilibrium of the actin cytoskeleton. (wikipedia.org)
Calponin 2's regulation of cell motility is based on inhibition of actin activated myosin motor function, as fibroblasts isolated from Cnn2 knockout mice showed increased cell traction force generated by myosin II motors. (wikipedia.org)
We recommend blocking with 2-5% BSA as we have found that use of 5% milk significantly reduces the band intensity for beta actin. (abcam.com)

Bafilomycin A1 induces caspase-independent cell death in hepatocellular carcinoma cells via targeting of autophagy and MAPK pathways. (targetmol.com)
The growth of the BEL-7402 hepatocellular carcinoma and HO-8910 ovarian cancer cell lines are retarded and the metastatic potential is inhibited by Bafilomycin A1. (targetmol.com)

Recent evidence implicates monocytes/macrophages in the etiopathology of cardiac fibrosis, but giving their heterogeneity and the antagonizing roles of macrophage subtypes in fibrosis, targeting these cells has been challenging. (nature.com)
By using single cell RNA sequencing analysis of immune cells in the same model, we establish the functional heterogeneity of macrophages and define an early pro-fibrogenic phase of NICM that is driven by Ccl5- expressing Ly6c high monocytes. (nature.com)
Macrophages are important immune cells that participate in both innate and adaptive immune responses, such as phagocytosis, recognition of molecular patterns, and activation of the immune response. (frontiersin.org)
Macrophages are immune cells widely distributed throughout the body and are components of innate immunity. (frontiersin.org)
Glycans are one of the four fundamental macromolecular components of all cells and are highly regulated in immune cells, including macrophages. (frontiersin.org)
Primary fibroblasts and peritoneal macrophages isolated from Cnn2 knockout mice migrate faster than that of wild type control cells. (wikipedia.org)
Deletion of calponin 2 in macrophages also significantly attenuated the development of atherosclerosis lesions in apolipoprotein E knockout (ApoE-/-) mice The expression of calponin 2 is significantly increased in cells cultured on hard versus soft gel substrates that produce high or low traction force and cytoskeleton tension. (wikipedia.org)
that is histopathological y very simi- T cel s, B cel s, natural kil er cel s, LMP1 of EBV can transform ro- lar to that caused by hepatitis B vi- macrophages, and dendritic cells, dent fibroblasts and is expressed rus (HBV) in humans, but it does so and this humanized mouse model in most of the human cancers as- through a different mechanism. (who.int)

CNN2 is expressed in a broader range of tissue and cell types, including developing and remodeling smooth muscle as well as adult mature smooth muscle, epidermal keratinocytes, fibroblasts, lung alveolar cells, endothelial cells, myeloid white blood cells, platelet, B lymphocyte, and myoblasts. (wikipedia.org)
A study showed that forced expression of calponin 2 in endothelial cells enhanced angiogenic cell migration in vivo and anti-sense calponin 2 RNA reduced chemotaxis of human umbilical vein endothelial cells in culture. (wikipedia.org)
Three major types of hCRPs are DAF , MCP , and CD59 , which are expressed mainly by endothelial cells in mammals. (ectrx.org)

In a microfluidic flow-based thrombosis assay, the time to initiation of rapid platelet/thrombus accumulation was significantly longer in blood samples from Cnn2 knockout versus wild type mice. (wikipedia.org)
1-(1-Methyl-1H-pyrrol-2-yl)-3-(5-(4-chlorophenyl)furan-2-yl)prop-2-en-1-one (7) and 1-(1-methyl-1H-pyrrol-2-yl)-3-(5-(2,5-dichlorophenyl)furan-2-yl)prop-2-en-1-one (9) were found to be the most potent antifungal agents against Candida krusei and therefore these compounds were chosen for flow cytometry analysis and Ames MPF assay. (anadolu.edu.tr)
ATP bioluminescence assay indicated that the antifungal activity of compounds 7 and 9 against C. krusei was significantly higher than that of other compounds and the reference drug (ketoconazole), whereas flow cytometry analysis revealed that the percentage of dead cells treated with compound 7 was more than that treated with compound 9 and ketoconazole. (anadolu.edu.tr)
MTT assay indicated that 1-(1-methyl-1H-pyrrol-2-yl)-3-(5-(2-nitrophenyl)furan-2-yl)prop-2-en-1-one (3) showed more selective anticancer activity than cisplatin against the HepG2 cell line. (anadolu.edu.tr)

Na avaliação biológica, utilizamos o teste de citotoxicidade com células de camundongo NIH-3T3 pelo ensaio MTT. (usp.br)
Os resultados mostraram que a citotoxicidade celular foi diretamente proporcional à concentração de porfirina 3MMe e tempo de incubação e irradiação, sendo o tratamento significativamente mais efetivo para as células da linhagem B16-F10 em relação às células da linhagem 3T3 e Melan-A. Não foi observada citotoxicidade celular na presença de porfirina 3MMe na ausência de luz (grupo controle), comprovando a ação fotodinâmica da formulação. (unip.br)

Eukaryotic expression vectors containing genes encoding plant proteins for killing of cancer cells. (weeksmd.com)
consequently, genes encoding some of these proteins are being used to design constructs for the inhibition of multiplying cancer cells. (weeksmd.com)
Activation domains influence the way proteins interact with the trancription apparatus of the cell (Dawson, 1995). (sdbonline.org)
Several genetic alterations have been described in SCCHN, including mutations in the p53 tumor suppressor gene and mutations in genes that encode cell cycle proteins such as p16 and cyclin D1. (oncotarget.com)
Matrix assisted laser desorption ionization (MALDI) mass spectrometry is commonly used to analyze biological molecules such as proteins, peptides and lipids from cells or tissue. (koreascience.kr)

Therefore, this study determined the cytotoxicity of KSO-SFE on colon cancer cells. (hindawi.com)
The whole-cell voltage clamp method ( 6 ) was chosen to measure swelling-induced chloride currents in isolated NIH 3T3 fibroblasts, colon carcinoma (CaCo) cells, and H9 cells, a human T cell lymphoma cell line (ATCC HTB 176). (biomedcentral.com)

One such peptide, Lunasin , has been shown to selectively act on newly transformed cells while having no cytotoxic effect on non-tumorigenic or established cancer cell lines. (weeksmd.com)

In addition, NK cells stimulated with lunasin plus cytokines displayed higher tumoricidal activity than those stimulated with cytokines alone using in vitro and in vivo tumor models. (weeksmd.com)
Chronic treatment with Bafilomycin A1 at the dose of 1 mg/kg significantly inhibits the tumor growth compared with controls, after 21 days[4]. (targetmol.com)
Using these inhibitors also significantly boosted the expression of the pro-apoptotic genes, Bax/Bcl-2 ratio as well as the expression of the tumor suppressor gene p53 in the MT-2 and Inf-3T3cells. (htlv.net)

RESULTS- Lcn2 is highly expressed by fat cells in vivo and in vitro. (diabetesjournals.org)
O presente trabalho teve como objetivo analisar e comparar o efeito de uma formulação micro e nanoencapsulada contendo porfirina 3MMe em linhagens de fibroblastos NIH 3T3, melanoma murino B16-F10 e melanócito Melan-A in vitro por meio de indicadores de função e viabilidade celular. (unip.br)

Paxillin, an integrin-assembly protein, has four major tyrosine phosphorylation sites, and the phosphorylation of Tyr31 and Tyr118 correlates with cell adhesion and migration. (silverchair.com)

Transfective over-expression of calponin 2 inhibited cell proliferation. (wikipedia.org)
Low levels of ROS can promote cell proliferation but high levels induce cell death [ 13 ]. (biomedcentral.com)
Furthermore, the effects of rigosertib, wortmannin, and rapamycin on the PI3K/Akt/mTOR pathway to inhibit the proliferation of ATLL cells were examined. (htlv.net)

The results showed that the cytotoxicity was directly proportional to the amount of porphyrin 3MMe and to time of incubation and irradiation, and significantly more effective treatment for cell line B16-F10 than the cell line 3T3 and Melan-A. It was not observe cellular cytotoxicity in the presence of porphyrin (3MMe) and absence of light (control group), proving the photodynamic action of the formulation. (unip.br)

Electrophysiological, fluorescence optical, and volume measurements were made to determine the effect of nucleoside analogs on the swelling-dependent chloride current (I Cl ) in NIH 3T3 fibroblasts and in human T cell lymphoma (H9) cells and the cAMP-dependent chloride current in CaCo cells. (biomedcentral.com)
Chinese hamster ovary (CHO) is one of the most widely used non-human mammalian cell lines. (thermofisher.com)
Human T lymphotropic virus type 1 (HTLV-1) infection can cause adult T-cell lymphoblastic leukemia (ATLL), an incurable, chemotherapy-resistant malignancy. (htlv.net)
HTLV-2 Encoded Antisense Protein APH-2 Suppresses HIV-1 Replication Abstract Antisense protein of Human T-cell. (htlv.net)
Animal models for human tumour mental animals is not easy to answer does induce adult T-cell leukaemia/ viruses that make use of animal virus- for these agents, because cancer bi- lymphoma (ATLL), albeit in monkeys es are scarce. (who.int)

Analysis of individual transfected cells by confocal microscopy reveals a pattern of distribution of PKR similar to that in Daudi cells, with immunostaining of cytoplasm and nucleoli. (pasteur.fr)

Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. (abcam.com)
Lipofectamine 3000 Transfection Reagent delivers a high efficiency for hard-to-transfect cell lines. (thermofisher.com)
When performing transfections in hard-to-transfect cell lines, Lipofectamine 3000 reagent can be an ideal choice. (thermofisher.com)
Lipofectamine 3000 reagent provided higher GFP transfection efficiency than Lipofectamine 2000 and FuGENE HD reagents for all five cell lines. (thermofisher.com)
The Lipofectamine 3000 transfection protocol was developed to be easy to use, while still ensuring optimum performance and reliability in a wide panel of cell lines. (thermofisher.com)
The easy protocol, exceptional plasmid DNA delivery, and the ability to effectively generate stable cell lines makes Lipofectamine 3000 reagent, the perfect reagent for CHO cell transfection experiments. (thermofisher.com)

The expression of calponin 2 in NIH/3T3 cells was decreased when cytoskeleton tension was reduced after blebbistatin inhibition of myosin II motors. (wikipedia.org)
Overexpression of IRS-1 resulted in inhibition of basal autophagy, and reduced oxidative stress-induced autophagy and cell death. (biomedcentral.com)
The IC50 of Bafilomycin A1 for inhibition of cell growth ranges from 10 to 50 nM[3]. (targetmol.com)

Peripheral blood mononuclear cells of healthy donors and post-transplant lymphoma patients were stimulated with or without lunasin in the presence of IL-12 or IL-2. (weeksmd.com)
The combination of lunasin and cytokines (IL-12 plus IL-2) was capable of restoring IFNÎ³ production by NK cells from post-transplant lymphoma patients. (weeksmd.com)
BACKGROUND: Frontline intensification (including consolidative whole-brain radiotherapy or high-dose chemotherapy with autologous stem-cell transplantation after induction therapy) has been proposed to treat primary central nervous system lymphoma (PCNSL). (bvsalud.org)

The results showed that mRNA expression of Akt/PI3K/mTOR was down-regulated in carrier, HAM/TSP, and ATLL patients, as well as MT-2, and Inf-3T3 cells, compared to the healthy individuals and untreated MT-2 and Inf-3T3 as controls. (htlv.net)
Treating the cells with rapamycin, wortmannin, and rigosertib decreased the phosphorylated forms of Akt and mTOR and restored their mRNA expression levels. (htlv.net)

Metabolic fluxes between membrane and water-soluble pool of choline-containing metabolites were assessed by exposing cells to [1,2- 13 C]choline for long and short periods of time to distinguish between catabolic and anabolic pathways in choline metabolism. (aacrjournals.org)

These cells participate in pathogen recognition, apoptotic cell clearance, and antigen presentation ( 14 ). (frontiersin.org)
Bafilomycin A1 induces the binding of Beclin 1 to Bcl-2, which further inhibits autophagy and promotes apoptotic cell death[1]. (targetmol.com)

The effect of calponin 2 on facilitating the velocity of cell adhesion was also shown with prostate cancer cells expression high or low levels of calponin 2. (wikipedia.org)
FN, a pivotal extracellular matrix component, was included for connecting to cell adhesion behavior. (bvsalud.org)
We found that mutation of Tyr31/118 caused enhanced activation of RhoA and premature formation of stress fibers with substantial loss of efficient membrane spreading and ruffling in adhesion and migration of NMuMG cells. (silverchair.com)
We found that Tyr31/118-phosphorylated paxillin competes with p190RhoGAP for binding to p120RasGAP, and provides evidence that p190RhoGAP freed from p120RasGAP efficiently suppresses RhoA activity during cell adhesion. (silverchair.com)
We conclude that Tyr31/118-phosphorylated paxillin serves as a template for the localized suppression of RhoA activity and is necessary for efficient membrane spreading and ruffling in adhesion and migration of NMuMG cells. (silverchair.com)

Adding lunasin to IL-12- or IL-2-stimulated NK cells demonstrated synergistic effects in the induction of IFNG and GZMB involved in cytotoxicity. (weeksmd.com)

Of note, ADP-1 decreased blood glucose levels and enhanced insulin production in pancreatic β cells in db/db mice. (phoenixpeptide.com)

Our results suggest that overexpression of IRS-1 promotes cells growth, inhibits basal autophagy, reduces oxidative stress-induced autophagy, and diminishes oxidative stress-mediated autophagy-dependent cell death. (biomedcentral.com)

The overexpression of TAK1 in HEK 293T cells decreased S6K1 phos phorylation in a dose dependent method and the quantification of p p70S6K1 S6K1 level was presented. (phosphorylaseinhibitors.com)

The expression patterns suggest that both genes are under the same regulatory control as are the E(spl) bHLH genes and thus, might play a part in Notch mediated cell differentiation. (sdbonline.org)
Unlike the other E(spl)-C genes, the gene is expressed within neuronal cells in the embryo. (sdbonline.org)
Inoculation with a high dose strains of LMP1 transgenic mice vide a powerful tool in mechanistic of EBV caused a B-cell lymphopro- were established that express LMP1 studies on the role of individual viral liferative disorder in these mice, under the control of the immunoglob- genes in cancer. (who.int)

Expression levels of PER2, a clock protein, were elevated in Sik3 -knockdown cells but down-regulated in Sik3 -overexpressing cells, which could be attributed to a phosphorylation-dependent decrease in PER2 protein stability. (elifesciences.org)
Evidence for GO induced autophagy includes increased levels of isoform B-II microtubule-associated protein 1 light chain 3 (LC3), aggregation of green fluorescence protein-tagged LC3, and increased numbers of autophagic vacuoles in cells. (biomedcentral.com)
in response to interferon treatment the protein kinase is induced severalfold in the cytoplasm whereas the level in the nucleus does not increase significantly. (pasteur.fr)
The expression of Lcn2 is induced during 3T3-L1 adipogenesis in a CCAAT/enhancer-binding protein-dependent manner. (diabetesjournals.org)
We also found that ADP-1 activates AMP-activated protein kinase (AMPK) in an adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1)-dependent pathway and stimulates glucose uptake in rat skeletal muscle cells (L-6 myotubes). (phoenixpeptide.com)
For two complexes with p K a values of 6.45 and 6.20 in protein containing solution, the emission lifetime increases very significantly following protonation. (worktribe.com)
The internal ribosome entry site (IRES) derived from the 5'-nontranslated region of the encephalomyocarditis virus exerts high-efficiency protein translation and is functional in a variety of cell culture systems. (ectrx.org)

On the other hand, 1-(1-methyl-1H-pyrrol-2-yl)-3-(5-(4-nitrophenyl)furan-2-yl)prop-2-en-1-one (1) was found to be more effective and selective on the A549 cell line than cisplatin. (anadolu.edu.tr)

Therefore, the tissue distributions of calponin 2 imply its potential role in regulating cytoskeleton functions and cell motility. (wikipedia.org)
A hypothesis is that a proper level of calponin 2 may be required to maintain the physiological motility of different cell types in different biological processes. (wikipedia.org)

This immediate effect can be substantially reduced by the simultaneous incubation of the cells with thymidine-5′-diphosphate (TDP) or uridine, both of which are by themselves unable to affect I Cl . (biomedcentral.com)

There was even a reduction in cell death. (thermofisher.com)

AZT and acyclovir block the swelling-dependent chloride current and the chloride flux in fibroblasts, and the regulatory volume decrease (RVD) and I Cl in H9 cells. (biomedcentral.com)

It is critically important to understand the molecular causes underlying these metabolic differences, because this may identify novel targets for attack in cancer cells. (aacrjournals.org)
0.001) of breast cancer cells compared with HMECs, indicating a higher metabolic flux from membrane phosphatidylcholine to choline and phosphocholine in breast cancer cells. (aacrjournals.org)
Choline kinase and phospholipase C were significantly overexpressed, and lysophospholipase 1, phospholipase A2, and phospholipase D were significantly underexpressed, in breast cancer cells compared with HMECs. (aacrjournals.org)
The magnetic resonance spectroscopy data indicated that elevated phosphocholine in breast cancer cells was primarily attributable to increased choline kinase activity and increased catabolism mediated by increased phospholipase C activity. (aacrjournals.org)
Over the past decade, one common feature consistently revealed by magnetic resonance (MR) spectroscopic studies is the elevation of phosphocholine (PC) and total choline-containing metabolites [tCho, glycerophosphocholine (GPC)+PC+free choline (Cho)] in cancer cells and solid tumors. (aacrjournals.org)
The aberrant choline phospholipid metabolism of breast cancer cells and its strong correlation with malignant progression has been observed in several studies. (aacrjournals.org)
Gene therapy has attracted attention for its potential to specifically and efficiently target cancer cells with minimal toxicity to normal cells. (weeksmd.com)
Numerous vectors have been engineered for the sole purpose of killing cancer cells, and some have successfully suppressed malignant tumours. (weeksmd.com)
Cancer cells produce greater levels of ROS than normal cells do because of increased metabolic stresses. (biomedcentral.com)
However, excessive production of ROS kills cancer cells. (biomedcentral.com)
It is not fully understood how cancer cells grow rapidly and survive in the presence of high ROS levels. (biomedcentral.com)
Squamous cell cancer of the head and neck (SCCHN) is the sixth leading cause for cancer deaths worldwide. (oncotarget.com)
Osteoclasts are bone-resorbing cells of monocyte/macrophage origin and are culprits of bone destruction associated with osteoporosis, rheumatoid arthritis, and cancer bone metastasis. (koreascience.kr)
One exception is hu- humanized SCID mice, the use of al oncogenic viruses that are strictly man T-cell lymphotropic virus type 1 surrogate hosts has not proven very species-specific, causing cancer in (HTLV-1): in addition to its ability to useful for defining tumour site con- humans only. (who.int)

This superior transfection performance allows you to reduce the reagent dose, thus helping lower toxicity risk when transfecting your cell line. (thermofisher.com)
We were very happy and surprised to see Lipofectamine® 3000 reagent provide a more than 10-fold difference in transfection efficiency in our difficult-to-transfect cell line. (thermofisher.com)

Two-dimensional isoelectric focusing analysis of PKR in whole cell extracts reveals the presence of multiple forms with different pI values whereas similar analysis of the nuclear fraction indicates only one predominant species with a relatively basic pI. (pasteur.fr)

The expression of calponin 2 decreases to lower levels in quiescent adult smooth muscle cells while the expression of calponin 1 is up-regulated. (wikipedia.org)
The purpose of this study was to determine how expression of Lcn2 is regulated in fat cells and to ascertain whether Lcn2 could be involved in metabolic dysregulation associated with obesity. (diabetesjournals.org)
These phenotypes were similar to those induced by RhoA(G14V) in parental cells, and could be abolished by expression of RhoA(T19N), Rac1(G12V), or p190RhoGAP in the mutant-expressing cells. (silverchair.com)
In addition, mTEC screen thymocytes for their expression of high affinity αβ-TCRs, with negative selection limiting the release of these reactive T-cells into the peripheral pool ( 4 ). (frontiersin.org)

Calponin 2 may affect cell migration differently in different cell types and in different biological processes. (wikipedia.org)
While adult lymphoid progenitor cells are produced by the bone marrow, their lineage commitment and development into mature T-cells is dependent on their migration into the thymus, where essential interactions with heterogeneous thymic stromal cells take place ( 1 ). (frontiersin.org)

In a quest for new therapeutic targets, our study sought to determine the levels of AKT, mTOR, and PI3K in ATLL MT-2 cells, HTLV-1 infected NIH/3T3 cells (Inf-3T3), and HTLV-1 infected patients (Carrier, HAM/TSP, and ATLL). (htlv.net)

Autophagy usually serves as a survival mechanism in response to stress conditions, but excessive induction of autophagy results in cell death. (biomedcentral.com)
Knockdown of autophagy-related gene 5 inhibited basal autophagy and diminished oxidative stress-induced autophagy and cell death. (biomedcentral.com)

Fibroblasts and CaCo cells were grown on glass cover slips in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal calf serum, 4 mM glutamine, 100 IU/ml penicillin, 100 µ g/ml streptomycin at 37°C, 5% CO 2 , 95% air, and measured 24-48 hr after splitting. (biomedcentral.com)
H9 cells were grown in suspension in RPMI-1640 medium supplemented with 10% fetal calf serum, 2 mM glutamine, 100 IU/ml penicillin, 100 µ g/ml streptomycin at 37°C, 5% CO 2 , 95% air. (biomedcentral.com)

Significant amounts of calponin 2 are found in blood cells of myeloid lineage. (wikipedia.org)

Monocytes derived from Cnn2 gene knockout mice proliferated faster than wild type control cells. (wikipedia.org)

Similar results are observed whether cells expressing wild-type PKR or a catalytically inactive mutant form of the kinase are analyzed, but untransfected 3T3 cells are not stained by the antibody used. (pasteur.fr)
These results suggest that the MCP + DAF and MCP + CD59 combinations could be more effective than DAF + CD59 in protecting the NIH 3T3 cells from injury caused by complement-dependent cytolysis, whereas MCP or DAF alone is stronger than CD59 alone in inhibiting membrane attack complex formation. (ectrx.org)
These results show that LMP1 , mour viruses classified by IARC in EBV-specific T-cell response. (who.int)

The presence of PC moieties significantly altered the adhesive response, including the number of attached cells, their morphologies, and nucleus shrinkage. (bvsalud.org)
Immunofluorescence of intact cells and quantitative immunoblotting of cell extracts indicate that PKR occurs in both the cytoplasm and the cell nucleus, with staining specifically in the nucleolus. (pasteur.fr)

In addition to intrathymic selection, and during this medullary residency period, CD4 + and CD8 + single positive (CD4SP, CD8SP) thymocytes acquire the ability to proliferate in response to TCR stimulation, and undergo licensing for cytokine production, prior to exiting the thymus to join the peripheral T-cell pool as Recent Thymus Emigrants (RTE) ( 5 , 6 ). (frontiersin.org)

Importantly, the overexpression of TAK1 KW showed the remark ably reduced binding affinity and significantly restored phosphor ylation level of S6K1 in contrast with TAK1 WT overexpression. (phosphorylaseinhibitors.com)

The phosphorylation pattern was comparable for the S6K1 phosphorylation pattern in complete cell lysates. (phosphorylaseinhibitors.com)

Anatomy33

Organisms15

Diseases7

Chemicals and Drugs60

Analytical, Diagnostic and Therapeutic Techniques and Equipment18

Phenomena and Processes44

Disciplines and Occupations3

Anthropology, Education, Sociology and Social Phenomena3

Humanities1

Information Science7

Named Groups1

Health Care7

Publication Characteristics1

Geographicals1

Fibroblast7

Stably transfected2

Hela4

Viability1

Adipocytes6

Apoptosis7

Tissues5

Cervical cancer1

Epithelial11

Endogenous4

Actin3

Hepatocellular carcinoma2

Macrophages8

Endothelial cells3

Assay4

Citotoxicidade2

Proteins5

Colon2

Cytotoxic1

Tumor3

Vitro2

Correlates with cell1

Proliferation3

Observe1

Human5

Confocal microscopy1

Lines6

Inhibition3

Lymphoma3

MRNA2

Pathways1

Apoptotic2

Adhesion5

Cytotoxicity1

Insulin1

Inhibits1

293T1

Genes3

Protein7

A5491

Motility2

Incubation1

Reduction1

Swelling-dependent1

Cancer14

Line2

Analysis1

Expression4

Migration2

MTOR1

Autophagy2

Serum2

Lineage1

Knockout1

Results3

Nucleus2

Peripheral1

Overexpression1

Phosphorylation1