• The aim of the present study is to explore in detail the effects of the oxindole Syk inhibitor OXSI-2 on various aspects of nigericin-induced inflammasome signaling. (elsevier.com)
  • Potential inflammasome agonists including ATP, nigericin and monosodium urate crystals did not stimulate IL-1β release from VSMCs. (open.ac.uk)
  • In addition, consistent with the model that loss of host cell potassium leads to egress through the activation of intraparasitic calcium fluxes, a previously isolated Toxoplasma mutant lacking a sodium hydrogen exchanger and defective in responding to calcium fluxes does not undergo nigericin-induced egress. (elsevier.com)
  • Our results show that nigericin-induced egress depends on an efflux of potassium from the cell and requires phospholipase C function and parasite motility. (elsevier.com)
  • Dissipation of the transmembrane electric potential by valinomycin did not have a significant effect on lactate accumulation, whereas abolishing the transmembrane pH gradient (delta pH) by nigericin prevented the accumulation and led to a rapid efflux of the accumulated acid. (nih.gov)
  • Immunoprecipitation of Cleaved-IL-1β (Asp117) from extracts of media from mouse bone marrow derived macrophages (mBMDM) treated with LPS #14011 (50 ng/ml, 4 hr) followed by nigericin (15 μM, 45 min). (cellsignal.com)
  • In macrophages, BCAP reduced the abundance of active caspase-1, maturation of the cytokine IL-1β, and cell death after exposure to the toxin nigericin or bacterial infection. (sciencemag.org)
  • Thus, the discovery that nigericin induces egress presents a novel assay that allows for the genetic and biochemical analysis of the signalling mechanisms that lead to the induction of motility and egress. (elsevier.com)
  • Nigericin decreases the viability of multidrug-resistant cancer cells and lung tumorspheres and potentiates the effects of cardiac glycosides. (medchemexpress.com)
  • When the pH in was lowered below 7 by treatment with nigericin, the viability decreased with decreasing pH in . (go.jp)
  • We activated inflammasomes in isolated neutrophils and PBMCs (LPS +/- ATP or Nigericin) and also performed autocrine/paracrine stimulation to evaluate the impact on neutrophil/PBMC activity. (ersjournals.com)
  • At the time points indicated by arrows, the following additions were made: mitochondria (all traces), 100 nm nigericin (all traces), and 200 nm FCCP (trace 2). (nih.gov)
  • Nigericin effectively causes intracellular parasites to exit their host cell within 30 min of treatment with the drug. (elsevier.com)
  • Nigericin also shows a decreased ability to form colonies under anchorage-independent conditions in a standard soft agar assay . (medchemexpress.com)
  • Cleavage of poly(ADP-ribose)polymerase caused by nigericin was greatly attenuated in MN9D/Bax cells suggesting that, like Bcl-2, Bax suppresses nigericin-induced cell death by inhibiting the activation of cysteine proteases. (elsevier.com)
  • Inorganic phosphate (A) and nigericin (B) increase the value of membrane potential but decrease the rate of ROS release. (nih.gov)
  • The difference in ROS production can be seen when the conditions change in the course of the experiment, producing qualitatively the same outcome (i.e. the addition of Pi or nigericin always resulted in decrease of the ROS generation rate despite the increase in ΔΨ (Fig. 5, A and B) because of trading the ΔpH component of proton motive force to the electric one). (nih.gov)
  • Typical treatment periods range from 3-24 hours at 37°C. Each investigator should adjust the concentration of Nigericin and treatment period to accommodate the particular cell line and research conditions. (immunochemistry.com)
  • Nevertheless, since the membrane remains intact during the treatment, in our nigericin-induced egress we are able to detect parasite-dependent permeabilisation of the host cell, a known step in induced egress. (elsevier.com)
  • To purify ZG membrane, the isolated ZGs were lysed with nigericin and centrifuged at 100,000 × g for 1 h. (mcponline.org)
  • Nigericin (0.1-10 nM) has apparently a dual effect on cell volume, that is a shrinking effect at lower Nigericin concentrations and a swelling effect at higher concentrations. (medchemexpress.com)
  • Starting with 24 hour samples and working backwards, 10 μM Nigericin was added to cells and the samples were incubated at 37°C throughout the induction process. (immunochemistry.com)
  • Nigericin requires signaling through pannexin-1 to induce caspase-1 maturation and IL-1β processing and release . (invivogen.com)
  • Pannexin-1 couples to maitotoxin- and nigericin-induced interleukin-1beta release through a dye uptake-independent pathway. (invivogen.com)
  • Enhancing MicroRNA Activity through Increased Endosomal Release Mediated by Nigericin. (harvard.edu)
  • New Mechanism for Release of Endosomal Contents: Osmotic Lysis via Nigericin-Mediated K+/H+ Exchange. (harvard.edu)
  • 2. Immediately prior to addition to the samples and controls, dilute 5 mM Nigericin stock 1:10 in diH 2 O to form a 500 µM working solution for use in treating samples. (immunochemistry.com)
  • Nigericin exhibits gteat toxicity for the HT29 and SW116 cell line with IC 50 of 12.92±0.25 μmol and 15.86±0.18 μmol. (medchemexpress.com)