NickelMolecular WeightChromatography, AffinityElectrophoresis, Polyacrylamide GelChromatography, Ion ExchangeChromatography, GelMolecular Sequence DataChromatographySubstrate SpecificityKineticsAmino Acid SequenceHydrogen-Ion ConcentrationHydrogenaseAmino AcidsChromatography, DEAE-CelluloseEscherichia coliCobaltIsoelectric PointUreaseMetalsDermatitis, Allergic ContactCloning, MolecularIsoelectric FocusingElectroplatingChromatography, High Pressure LiquidCrystallizationMetallurgyHydroxyapatitesPatch TestsBacterial ProteinsRecombinant ProteinsChromiumSolubilityEnzyme StabilityBase SequenceMetals, HeavyAmmonium SulfateTemperatureCations, DivalentChemical PrecipitationImmunodiffusionDurapatiteMacromolecular SubstancesCattleSpectrophotometryChemistryCentrifugation, Density GradientMetalloproteinsSequence Homology, Amino AcidChemical PhenomenaManganeseChromatography, AgaroseSepharoseHistidineAlcaligenesHot TemperatureProtein BindingCrystallography, X-RayOxidoreductasesCarbohydratesZincSpectrophotometry, AtomicElectrophoresis, DiscLiverAldehyde OxidoreductasesMagnesiumDetergentsRabbitsStainless SteelSpectrophotometry, UltravioletPlasmidsMass SpectrometryBinding SitesHydrolysisOrthodontic AppliancesEdetic AcidCatalysisCell LineMethodsCarrier ProteinsTrace Elements