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  • tissues
  • Dedifferentiation of stump tissues, such as skeletal muscle, precedes blastema formation, but it was not known whether dedifferentiation involves stem cell activation. (rupress.org)
  • We demonstrate that skeletal muscle dedifferentiation involves satellite cell activation and that these cells can contribute to new limb tissues. (rupress.org)
  • It is unclear to what extent differentiated cells reverse mature phenotypes and to what extent undifferentiated cells, such as stem cells, residing within differentiated tissues become activated, followed by their incorporation into the blastema. (rupress.org)
  • Furthermore, targeting specific cell types will be necessary to understand how regeneration of the diverse tissues within the limb is accomplished. (biologists.org)
  • This database contained DNA sequence information of genes from 19 different tissues, including different regenerating organs and limb blastemas. (phys.org)
  • Its existence in newt tissues was confirmed using both DNA- and protein-based methods. (phys.org)
  • By looking at the expression of Newtic1 in larval newt tissues and limb blastemas, the researchers determined that Newtic1-expressing erythrocytes do not contribute to the limb blastema in the larval stage. (phys.org)
  • After the limb or tail has been autotomized, cells move into action and the tissues will regenerate. (wikipedia.org)
  • regrow
  • Well, Ben, when Billy Shakespeare had those witches in Macbeth call for an "eye of newt, and toe of frog" to complete a very special hell-broth recipe, I'm not sure he knew those amphibians could actually regrow those body parts. (baynature.org)
  • vertebrate
  • We documented an absolute requirement for macrophages in the orchestration of postamputation immune microenvironment and blastema formation, providing direct evidence for the immunological control of successful regeneration in an adult vertebrate. (pnas.org)
  • Ambystoma
  • To investigate the role of macrophages during scar-free tissue repair in an efficiently regenerating model, we dissected the inflammatory response to injury in regenerating limbs of the axolotl aquatic salamander ( Ambystoma mexicanum ) at the cellular and molecular level. (pnas.org)
  • This research was designed to follow up the observation of Thornton and Kraemer ('51) that regressed, denervated limbs of Ambystoma larvae will not regenerate upon reinnervation if all digits on the limbs were not completely resorbed. (umich.edu)
  • Heparan sulfates mediate positional information by position-specific growth factor regulation during axolotl ( Ambystoma mexicanum ) limb regeneration. (springer.com)
  • In this study, we have established efficient CRISPR/Cas9 mediated gene knockin approaches in the axolotl ( Ambystoma mexicanum ), which has allowed us to genetically mark two critical stem cell pools for limb and spinal cord regeneration. (pnas.org)
  • wound
  • Epimorphic limb regeneration proceeds by rapid wound closure and is critically dependent on the formation of a multipotent mesenchymal growth zone, the blastema, which gives rise to the newly formed limb ( Wallace, 1981 ). (rupress.org)
  • Systemic macrophage depletion during this period resulted in wound closure but permanent failure of limb regeneration, associated with extensive fibrosis and disregulation of extracellular matrix component gene expression. (pnas.org)
  • First, the animal heals the wound at the site of the missing limb. (petersons.com)
  • regenerates
  • The regenerates that did form were strongly deviated (45-90°) radially from the longitudinal axis of the limb. (umich.edu)
  • regeneration in mammals
  • Given that these cell types interact to form inhibitory barriers in mammals, identifying the mechanisms underlying their permissive behaviors in the newt will provide new insights for improving spinal cord regeneration in mammals. (biomedcentral.com)
  • Anoop Kumar and colleagues from University College London (UCL), writing in the journal Science, said the finding 'may hold promise for future efforts to promote limb regeneration in mammals. (yahoo.com)
  • induces
  • Because of the restricted expression of Shh to the posterior margin, it remains unclear, however, whether Shh induces the limb polarity directly or through a secondary signal. (biomedcentral.com)
  • digits
  • Overexpression of BMP-2 in regenerating limbs caused a loss of digits. (biomedcentral.com)
  • Overexpression of Noggin ( BMP inhibitor) in regenerating limbs also resulted in a loss of digits. (biomedcentral.com)
  • Such injuries - the partial or complete loss of digits or limbs and deforming facial injuries - have profoundly affected the quality of life of the wounded as well as presented a new set of challenges for the medical community faced with treating them. (medgadget.com)
  • The proposed studies address one of the most pressing needs of the wounded soldier population: the replacement of lost digits and limbs. (pitt.edu)
  • distal
  • One limb served as a normal regeneration control, and in the other limb the third digit from the removed hand was implanted in place of the removed radius, so that the three distal phalangeal segments protruded past the plane of amputation. (umich.edu)
  • vertebrates
  • Newts are the only four-legged vertebrates that can regenerate their body parts, even as adults. (phys.org)
  • Can this remarkable ability be explained by genes shared by vertebrates, including humans, or by unique genes that the newt may have evolved? (phys.org)
  • gene
  • In a study published in Scientific Reports , researchers at several Japanese universities, including the University of Tsukuba, and the University of Dayton, report the discovery of a novel gene, Newtic1, from the blastema of an adult newt. (phys.org)
  • genes
  • In the study, the researchers constructed a database of all the protein-coding genes from the Japanese fire-bellied newt that had been reported in the Japan newt research community. (phys.org)
  • FV vectors are efficient mean of transferring genes into axolotl limb/tail and infection persists throughout regeneration and reamputation. (biomedcentral.com)
  • Zool
  • McLaughlin, H.M.G., Rathbone, M.P., Liversage R.A., and D.S. McLaughlin, 1983, Levels of cyclic GMP and of cyclic AMP in regenerating forelimbs of adult newts following denervation, J.Exp.Zool. (springer.com)
  • proximal
  • Retinoic acid (RA) is able to respecify the positional identity of the blastema to a more proximal value, thus raising the possibility that the RA response system is activated during limb regeneration. (ucl.ac.uk)
  • mammalian
  • Although many features of mammalian cytokine/chemokine signaling are retained in the axolotl, they are more dynamically deployed, with simultaneous induction of inflammatory and anti-inflammatory markers within the first 24 h after limb amputation. (pnas.org)
  • Injection of EGFP-expressing FV but not lentivirus vector particles into regenerating limbs and tail resulted in widespread expression that persisted throughout regeneration and reamputation pointing to the utility of FV for analyzing adult phenotypes in non-mammalian models. (biomedcentral.com)
  • inhibition
  • The inhibition of sonic hedgehog signaling with cyclopamine caused hypomorphic limbs (during development and regeneration) but did not affect the expression of BMP-2 and SOX-9 . (biomedcentral.com)
  • Inhibition and axial deviation of limb regeneration in the newt by means of a digit implanted into the amputated limb Supported by grants from the Damon Runyan Foundation and the Muscular Dystrophy Association. (umich.edu)