Neuronal embryonal carcinoma stem cells. Medical search. Frequent questions

Cell cultures containing undifferentiated stem cells were isolated from the primary tumor which have a euploid karyotype. (wikipedia.org)
We compared microRNA expression patterns in three cisplatin resistant sublines derived from paternal cisplatin sensitive germ cell tumor cell lines in order to improve our understanding of the mechanisms of cisplatin resistance. (biomedcentral.com)
Three cisplatin resistant sublines (NTERA-2-R, NCCIT-R, 2102EP-R) showing 2.7-11.3-fold increase in drug resistance after intermittent exposure to increasing doses of cisplatin were compared to their parental counterparts, three well established relatively cisplatin sensitive germ cell tumor cell lines (NTERA-2, NCCIT, 2102EP). (biomedcentral.com)
The hsa-miR-371-373 cluster (counteracting cellular senescence and linked with differentiation potency), as well as hsa-miR-520c/-520h (inhibiting the tumor suppressor p21) were 3.9-16.3 fold up-regulated in two of the three cisplatin resistant cell lines. (biomedcentral.com)
We created an in vitro model of acquired cisplatin resistance by long term exposure of three well established germ cell tumor cell lines to cisplatin, resulting in sublines with significantly increased resistance to cisplatin. (biomedcentral.com)
The paternal cell lines are (1) the p53-wt (wild type) pluripotent gonadal germ cell tumor cell line NTERA-2, (2) the p53-mutated pluripotent extragonadal nonseminomatous germ cell tumor cell line NCCIT and (3) the p53-wt nullipotent embryonal cell carcinoma 2102EP, derived from a primary human testicular teratocarcinoma. (biomedcentral.com)
Medulloblastoma is defined by the World Health Organization (WHO) as "an embryonal neuroepithelial tumor arising in the cerebellum or dorsal brainstem, presenting mainly in childhood and consisting of densely packed small round undifferentiated cells with mild to moderate nuclear pleomorphism and high mitotic count. (medscape.com)
The medulloblastoma has been defined by the World Health Organization (WHO) as a "malignant, invasive embryonal tumor of the cerebellum with preferential manifestation in children, predominantly neuronal differentiation, and inherent tendency to metastasize via CSF [cerebrospinal fluid] pathways. (medscape.com)
PHD2 is certainly the major prolyl hydroxylase that adjusts HIF-1 proteins balance in nonhypoxic cells, whereas knockdown of PHD1 or PHD3 will not really affect HIF-1 proteins amounts in many tumor cell lines (Berra range 350C1700 at a quality of 30,000. (forgetmenotinitiative.org)
Medulloblastoma is the most common embryonal brain tumor, whereas other embryonal tumor types are considered "rare" and typically affect infants and very young children. (medlink.com)
WHO CNS5 defines three main entities: embryonal tumor with multilayered rosettes (ETMR), CNS neuroblastoma FOXR2 -activated, and CNS tumor with BCOR internal tandem duplication. (medlink.com)
Recent advances in the molecular diagnosis and treatment of pineoblastoma, a rare embryonal tumor of the pineal gland with particular penetrance in infants and young children, are also highlighted in this review. (medlink.com)
The common embryonal brain tumor medulloblastoma and the rare atypical rhabdoid teratoid tumor are well-defined entities in terms of their histopathological features, immunophenotype, and genetic profiles, as are other brain tumors occurring in this age range, including choroid plexus tumors and infant gliomas, and they are discussed in separate articles. (medlink.com)
03/01/2011 [-] IgG4 plasma cells in inflammatory myofibroblastic tumor inflammatory marker or pathogenic link? (infopig.com)
Nestin stabilizes lamin A-C for maintaining nuclear integrity and protecting tumor cells from senescence. (cusabio.com)
Also, it is been detected in BC stem cells and newly-formed tumor vessels promoting invasion and metastasis. (cusabio.com)
One strategy to increase cure rates is the identification of patient-specific drug responses in tissue models that mimic the interaction between patient cancer cells and tumor environment. (regenhu.com)
A gelatin-methacrylate/fibrin-based matrix containing multiple cell types mimics the tumor-microenvironment that promotes spontaneous micro-vessel formation by embedded endothelial cells. (regenhu.com)
We demonstrate that micro-vessels are attracted by and grow into tumor spheroids and that neuroblastoma cells invade the tumor-environment as soon as the spheroids disrupt. (regenhu.com)

Also, exposing P19 cells to retinoic acid (RA) can differentiate them into neuronal cells. (wikipedia.org)
The difference between these subtype cell lines is the ability to differentiate into neuronal cells or muscle cells in response to treatment with retinoic acid or DMSO, respectively. (wikipedia.org)
Because the concentration of retinoic acid or DMSO is nontoxic to the cells, the drug-specific differentiation is due to induction of cells not selection. (wikipedia.org)
Treatment of undifferentiated P19 cells with retinoic acid can specifically induce them into neuronal cells. (wikipedia.org)
They can be differentiated into other cell types when exposed to non-toxic drugs such as retinoic acid and dimethyl sulfoxide (DMSO) [1]. (cytion.com)
Retinoic acid induces the development of neurons, microglia, and astroglia, whereas DMSO initiates beating cardiomyocytes and smooth muscle cell development from P19 cells. (cytion.com)
The P19 embryonal carcinoma cells differentiate into neurons, astrocytes, and fibroblast-like cells following induction with retinoic acid. (huji.ac.il)
Expression analysis of the human Hox Complex in teratocarcinoma cells (N-tera2/D1) upon treatment with retinoic acid and the definition of temporal colinearity concept. (cnr.it)
摘要： Objective To investigate the regulatory mechanisms of acetylated p53 in the expression of microtubule-associated protein-2 (MAP2) in neuronal differentiation of P19 cells induced by all-trans retinoic acid (RA). (cams.cn)
Role of Acetylated p53 in Regulating the Expression of map2 in Retinoic Acid-induced P19 Cells[J].Chinese Medical Sciences Journal, 2010, 25(2): 71-75. (cams.cn)
Then, after overexpressing the SPTBN4-miR1 precursor in NT2 cells and retinoic acid treatment, the expression of pluripotent and differentiation revealed the role of SPTBN4-miR1-5p and SPTBN4-miR1-3p in promoting differentiation and exclusion from the pluripotent state. (ac.ir)

TWS 119 induces neuronal differentiation in pluripotent murine embryonal carcinoma cells and embryonic stem cells at 400 nM. (tocris.com)
P19 is a murine embryonal carcinoma cell line. (cytion.com)
No immunoreactivity is seen with murine EC, EG or ES cells. (sigmaaldrich.com)

P19 cells is an embryonic carcinoma cell line derived from an embryo-derived teratocarcinoma in mice. (wikipedia.org)
For developmental biologists, embryonal carcinoma, which is derived from teratocarcinoma, is a good object for developmental study. (wikipedia.org)
P19 is a type of pluripotent embryonal carcinoma cells originally obtained from teratocarcinoma developed in a C3H/He mouse. (cytion.com)
Furthermore, when cells from such an 'adapted' culture were inoculated into a SCID (severe combined immunodeficient) mouse, we obtained a teratocarcinoma containing histologically recognizable stem cells, which grew out when the tumour was explanted into culture and exhibited properties of the starting ES cells. (silverchair.com)
Molecular mechanism controlling the expression of the HOX Complex gene family in human teratocarcinoma cells (N-tera2/D1). (cnr.it)

In fact, low levels of telomerase activity have been found in human adult stem cells including haematopoietic and non-haematopoietic stem cells such as neuronal, skin, intestinal crypt, mammary epithelial, pancreas, adrenal cortex, kidney, and mesenchymal stem cells (MSCs) ( Table 1 ). (nature.com)
Functional profiling reveals critical role for miRNA in differentiation of human mesenchymal stem cells. (ac.ir)
We demonstrate that both, adipocyte- and iPSC-derived mesenchymal stem cells can guide this process. (regenhu.com)

TWS 119 enhances the proliferation and survival of γδ T-cells, and induces differentiation of CD8+ T cells towards a stem cell-like memory T cell phenotype at 2.5 μM. (tocris.com)
After characterization of the different stages of cell differentiation into neuronal- and glial-like phenotype toxicity studies were performed to evaluate whether this model would be suitable for developmental neurotoxicity studies. (johnshopkins.edu)
Moreover, we describe for the first time an association of the up-regulation of micro-RNA species such as hsa-miR-512-3p/-515/-517/-518/-525 and down-regulation of hsa-miR-99a/-100/-145 with a cisplatin resistant phenotype in human germ cell tumors. (biomedcentral.com)
We further suggest that the P19 cell line can serve as a model system for the study of neurotransmitter phenotype acquisition and plasticity throughout neuronal differentiation. (huji.ac.il)

Studies have shown that a certain concentration of RA can induce P19 cells to differentiate into neuronal cells, including neurons and glial cells, whereas 0.5% - 1% DMSO led P19 cells to differentiate into cardiac or skeletal muscle cells. (wikipedia.org)
After 10 days of exposure, astroglial cells can be detected using glial fibrillary acidic protein (GFAP), which is a specific marker of glial cells. (wikipedia.org)
The major advantage of the neuronal cell culture models derived from human stem cells is their ability to replicate the crucial stages of neurodevelopment such as the commitment of human stem cells to the neuronal lineage and their subsequent stages of differentiation into neuronal and glial-like cell. (johnshopkins.edu)
In these studies we used mixed neuronal/glial culture derived from the NTERA-2 (NT-2) cell line, which has been established from human pluripotent testicular embryonal carcinoma cells. (johnshopkins.edu)
The toxicity was then evaluated by measuring the mRNA levels of cell specific markers (neuronal and glial). (johnshopkins.edu)
In contrast, MetHgCl was toxic for both cell types, neuronal and glial, as mRNA specific for astrocytes and neuronal markers were affected. (johnshopkins.edu)
Peripherally administered EPO crosses the blood-brain barrier and activates in the brain anti-apoptotic, anti-oxidant and anti-inflammatory signaling in neurons, glial and cerebrovascular endothelial cells and stimulates angiogenesis and neurogenesis. (biomedcentral.com)

Adult p107-null mice had elevated numbers of proliferating progenitor cells in their lateral ventricles. (rupress.org)
EPO supports the proliferation and differentiation of erythroid progenitor cells and is critical for their survival [ 1 ]. (biomedcentral.com)
Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells. (cusabio.com)
Interstitial microvoids enhanced differentiation of multiple stem/progenitor cells (vascular, mesenchymal, neural), otherwise not possible with conventional bulk hydrogels. (regenhu.com)

We have shown that human CR-1 is overexpressed in approximately 40-90% of a variety of human carcinomas including breast tumors. (grantome.com)
Our approach was designed to characterize the role of micro-RNAs on the presumably multifactorial phenomenon of acquired cisplatin resistance in germ cell tumors. (biomedcentral.com)
Human embryonal carcinoma (EC) cells are the stem cells of teratocarcinomas, and they are key components of germ cell tumors (GCTs). (sigmaaldrich.com)
Resolving the Pathogenesis of Anaplastic Wilms Tumors through Spatial Mapping of Cancer Cell Evolution. (lu.se)
The World Health Organization 2021 Classification (WHO CNS5), based on an integrated taxonomy with a strong emphasis on molecular profiling, established two types of embryonal tumors: medulloblastomas and other CNS embryonal tumors. (medlink.com)
In contrast to medulloblastomas, which by definition originate from the cerebellum or dorsal brainstem, other CNS embryonal tumors may arise across the neuraxis. (medlink.com)
Due to the unique features of the population affected by these entities, rare embryonal tumors deserve specific understanding, comprehensive diagnostic tools, and the development of much-needed novel tailored treatment approaches prioritizing less-toxic therapies to the immature nervous system. (medlink.com)
In this article, the authors provide an overview of current concepts of clinicopathologic characteristics, specific molecular diagnosis, and general treatment strategies for these rare embryonal tumors of childhood. (medlink.com)
Rare embryonal tumors may be congenital and can arise along the neuraxis. (medlink.com)
Choriocarcinoma is the rarest of the germ cell tumors. (testicularcancersociety.org)
Some tumors, such as brain stem gliomas, cerebellar pontine angle tumors, fourth ventricle tumors, and pineal area tumors extend into the cerebellum from surrounding areas. (clinicalgate.com)

Indeed, exposing aggregated P19 cells to dimethyl sulfoxide (DMSO) induces differentiation into cardiac and skeletal muscle. (wikipedia.org)
Synaptic network activity induces neuronal differentiation of adult hippocampal precursor cells through BDNF signaling. (mdc-berlin.de)

It is widely used in biomedical research, mainly for studying developmental biology, stem cell biology, cell differentiation, and drug screening. (cytion.com)
Thus, P19 cells can be useful in studying cell differentiation and developmental processes. (cytion.com)
The results obtained suggest that a neuronal mixed culture derived from human NT2 precursor cells is a suitable model for developmental neurotoxicity studies and gene expression could be used as a sensitive endpoint for initial screening of potential neurotoxic compounds. (johnshopkins.edu)
Of particular interest is their contribution to Developmental Neurotoxicity (DNT) as well as their potential impact on stem cells. (plos.org)
Heterozygous necdin-deficient mice (B6.Cg-Ndn tm1ky ) were bred from wild-type (WT) females to generate WT (+m/+p) and heterozygotes (+m/−p) animals, which were examined of LC-NE neuronal activity, developmental reflexes, and plethysmography. (biomedcentral.com)

Adaptive peripheral immune response increases proliferation of neural precursor cells in the adult hippocampus. (mdc-berlin.de)
NMDA and benzodiazepine receptors have synergistic and antagonistic effects on precursor cells in adult hippocampal neurogenesis. (mdc-berlin.de)
Differential 24 h responsiveness of Prox1-expressing precursor cells in adult hippocampal neurogenesis to physical activity, environmental enrichment, and kainic acid-induced seizures. (mdc-berlin.de)

Moreover, signaling pathways related to neurogenesis and myogenesis were also investigated by studying gene expression or generating mutants of P19 cells. (wikipedia.org)
Intermediate progenitors in adult hippocampal neurogenesis: Tbr2 expression and coordinate regulation of neuronal output. (mdc-berlin.de)

Cholinergic markers, such as the vesicular acetylcholine transporter, acetyl cholinesterase, and choline acetyltransferase, are expressed in about 85% of the cells in sparse cultures and are largely suppressed at high cell densities. (huji.ac.il)

Human embryonic stem cells (hESCs) possess the ability to self-renew in an undifferentiated state in culture while retaining the ability to differentiate into all of the cell types in the human body. (biomedcentral.com)

Other than into neurons and astrocytes, P19 cells can also differentiate to oligodendrocytes, which can be detected using the specific markers, myelin-associated glycoprotein and 2',3'-Cyclic-nucleotide 3'-phosphodiesterase. (wikipedia.org)
Whilst LiCl exposure did not significantly alter the proportion of cells expressing markers for stem cells/undifferentiated cells (Oct4, SSEA4), neurons (Neurofilament M), astrocytes (GFAP) or cell cycle phase, the drug caused a 1.4-fold increase in total cell number. (plos.org)

These mouse carcinoma cells possess an epithelial-like morphology. (cytion.com)
We have also found that overexpression of either Cr-1 or CR-1 in mouse mammary epithelial cells in vitro and in vivo as a transgene results in their transformation and in their enhanced ability to migrate and invade as a result of epithelial-mesenchymal transition (EMT). (grantome.com)
In vitro studies indicate that Dab2 establishes epithelial cell polarity and organization by directing endocytic trafficking of membrane glycoproteins. (biomedcentral.com)

Several neuronal markers such as neurofilament proteins, HNK-1 antigen and tetanus toxin binding sites are expressed at highest levels during these days. (wikipedia.org)
Furthermore the Cdo-deficient dorsal forebrain shows more powerful Wnt signalling activity improved cell proliferation and improved manifestation from the dorsal markers and Wnt focuses on Pax6 Gli3 Axin2. (cell-metabolism.com)
Using immunocytochemistry, flow cytometry, qRT-PCR and MPSS, we found that all three cell lines actively proliferated and expressed similar "stemness" markers including transcription factors POU5F1/Oct3/4 and NANOG, glycolipids SSEA4 and TRA-1-81, and alkaline phosphatase activity. (biomedcentral.com)
Review: Evaluation of pancreatic cancer stem cells-related markers CD133, CD44, Oct4, and Nestin proteins in PANC-1 and SW1990 cells by western blotting. (cusabio.com)

Modulation of dynamics in a pre-existing hippocampal network by neural stem cells on a microelectrode array, J. Neural Eng. (u-tokyo.ac.jp)
Nestin, originally identified as a marker of neural stem cells, was demonstrated to be expressed in breast cancer (BC). (cusabio.com)

Perturbation of PRTG activity in P19 embryonal carcinoma cells and in chick embryos, by either RNA interference or a dominant-negative PRTG mutant, increases neuronal differentiation. (nycu.edu.tw)
Embryonal carcinoma (EC) cells are the stem cells of teratocarcinomas, and the malignant counterparts of embryonic stem (ES) cells derived from the inner cell mass of blastocyst-stage embryos, whether human or mouse. (silverchair.com)
It is also essential to understand how the inherited variation in the sex, stage, quality and genetic background of embryos, as well as environmental influences such as derivation methods and passage procedures can affect the ability of hES cell lines to self-renew and differentiate. (biomedcentral.com)

This study explores the use of a simple human neurosphere-based in vitro model to characterise the pharmacological and toxicological effects of LiCl and VPA using gene expression changes linked to phenotypic alterations in cells. (plos.org)
Human embryonic stem cells (hESc) can also be used to analyze the in vitro development from undifferentiated pluripotent cells leading to terminally differentiated cell types, recapitulating the process of early embryonic development [5] . (plos.org)
On prolonged culture in vitro , human ES cells acquire karyotypic changes that are also seen in human EC cells. (silverchair.com)
In vitro neurosphere assays revealed striking increases in the number of neurosphere forming cells from p107 −/− brains that exhibited enhanced capacity for self-renewal. (rupress.org)

Lines I3 and I6 possess normal XX and a normal XY karyotype while BG01V is a variant cell line with an abnormal karyotype derived from the karyotypically normal cell line BG01. (biomedcentral.com)
Mapping of the KARYOTYPE of a cell. (lookformedical.com)

Using doses between 1 μM to 3 μM of RA can generate neurons as the most abundant cell type. (wikipedia.org)
Advancement of the central anxious system (CNS) is really a multistep procedure that will require coordinated occasions including cell WK23 fate standards proliferation of neural progenitors (NPCs) and terminal differentiation to create adequate amounts of adult neurons. (cell-metabolism.com)
The cells mature into functional neurons, as determined by their ability to release neurotransmitters in a Ca 2+ - and depolarization-dependent manner. (huji.ac.il)
The factor mediating the density effect is concentrated exclusively on the cell membrane of P19 neurons and not on the nonneuronal cells, which also differentiate from P19 embryonal carcinoma cells. (huji.ac.il)
Melatonin modulates cell survival of new neurons in the hippocampus of adult mice. (mdc-berlin.de)

Histologic subtypes of medulloblastoma include: (1) desmoplastic/nodular type, (2) medulloblastoma with extensive nodularity, and (3) large-cell/anaplastic medulloblastoma. (medscape.com)
1] Several subtypes of medulloblastoma have been recognized including: (1) desmoplastic/nodular type, (2) medulloblastoma with extensive nodularity, (3) large-cell variant, and (4) anaplastic medulloblastoma. (medscape.com)

Researchers use P19 cells to elucidate cell signaling pathways and cellular and molecular mechanisms involved in these processes. (cytion.com)
Consequently furthermore to advertising ventral central anxious program cell fates with Shh Cdo promotes neuronal differentiation by suppression of Wnt signalling and a direct hyperlink between two main dorsoventral morphogenetic signalling pathways. (cell-metabolism.com)
A critical length of telomere repeats is required to ensure proper telomere function and avoid the activation of DNA damage pathways that result in replicative senescence or cell death. (nature.com)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)

P19 cells can be maintained in exponential growth because of a stable chromosomal composition. (wikipedia.org)
The chromosomal constitution of cells which deviate from the normal by the addition or subtraction of CHROMOSOMES, chromosome pairs, or chromosome fragments. (lookformedical.com)

Due to the pluripotency of P19 cells, those new derived cell lines can be ectoderm, mesoderm and endoderm-like cells. (wikipedia.org)
Because embryonal carcinoma can differentiate into cells of all three germ layers, P19 cells can also differentiate into those ectoderm, mesoderm and endoderm-like cells. (wikipedia.org)

Cell Communication and Signaling 8 : 12. (mdc-berlin.de)

In these features, the 'adapted' ES cells resembled malignant EC cells. (silverchair.com)

Role of Otx2 in the control of identity and fate of neuronal precursors in the ventral midbrain. (cnr.it)

However, the possible differences in proliferation and differentiation capabilities among independently-derived human embryonic stem cells (hESCs) are not well known because of insufficient characterization. (biomedcentral.com)

In addition, in order to phenotypically anchor the gene expression data, changes in the heterogeneity of cell subtype populations and cell cycle phase were monitored using flow cytometry. (plos.org)
p300/CBP associated factor (PCAF) was found induced in RA-treated cells and enriched in the nucleus, which might contribute to the acetylation of p53 in the regulation of map2 gene. (cams.cn)
The SPTBN4 gene, a part of the spectrin protein family, plays important roles in various cellular processes, including cell cycle, nerve cell development, and so on. (ac.ir)
However, a profound variation in colony morphology, growth rate, BrdU incorporation, and relative abundance of gene expression in undifferentiated and differentiated states of the cell lines was observed. (biomedcentral.com)
A fragment of the Dab2 human cDNA was also isolated based on its frequent loss of expression in ovarian cancer, and was termed DOC-2 (Differentially expressed in ovarian carcinoma gene 2) [ 2 ]. (biomedcentral.com)

In keeping with its appearance pattern Cdo has important assignments in myogenesis and neurogenesis12 13 In myoblasts Cdo promotes differentiation as an element of multiprotein complexes offering the cell adhesion substances N-cadherin as well as the carefully related proteins Boc and Neogenin14-16. (cell-metabolism.com)
Here we used an affinity-purification mass spectrometry-based (AP-MS) approach to identify novel and particularly intracellular sGAG-interacting proteins in human bone marrow stromal cells (hBMSC). (degruyter.com)
Enrichment analysis for protein localization showed that mainly intracellular and cell-associated interacting proteins were identified. (degruyter.com)
Co-IP assays Cells had been lysed in altered radioimmunoprecipitation assay stream, and WCLs had been incubated over night with anti-FLAG (Sigma-Aldrich, St. Louis, MO), anti-PHD2 (Novus Biologicals, Littleton, Company), anti-PHD3 (Novus Biologicals), or anti-hydroxyproline (Abcam) antibody in the existence of proteins ACagarose beans (Novus Biologicals). (forgetmenotinitiative.org)
Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. (cusabio.com)

Here we show a novel function for Retinoblastoma family member, p107 in controlling stem cell expansion in the mammalian brain. (rupress.org)

This antibody reacts with TRA-1-60 antigen that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). (sigmaaldrich.com)

The cell line is pluripotent and can differentiate into cell types of all three germ layers. (wikipedia.org)
Also, it is the most characterized embryonic carcinoma (EC) cell line that can be induced into cardiac muscle cells and neuronal cells by different specific treatments. (wikipedia.org)
This section will cover the following: What is P19 cell line? (cytion.com)
The cell line was first established in 1982 by McBurney and Rogers. (cytion.com)
The P19 cell line is widely cultured in research laboratories due to its unique characteristics. (cytion.com)
How do you culture P19 cell line? (cytion.com)
Is P19 an adherent cell line? (cytion.com)
The doubling time reported for the P19 cell line is approximately 2 to 3 days. (cytion.com)
P19 embryonic carcinoma cell line is adherent. (cytion.com)
A humidified incubator set at 37°C with a 5% CO2 supply is essential to growing and culturing the P19 embryonic carcinoma cell line. (cytion.com)
Biosafety level 1 laboratory settings are required for the P19 cell line. (cytion.com)
This section will discuss the advantages and disadvantages of the P19 cell line. (cytion.com)
Pluripotent embryonic carcinoma cell line P19 is a useful model for studying early embryonic development. (cytion.com)
Thus, telomerase activity and telomere maintenance are associated with the immortality of cancer cells, germ-line cells, and embryonic stem (ES) cells. (nature.com)
LINE-1 retrotransposons drive human neuronal transcriptome complexity and functional diversification. (lu.se)
The aim of the present study was to investigate the expression of this miRNA, called SPTBN4-miR1 , in the process of differentiation of human embryonal carcinoma cell line NT2 and also the overexpression effect of this miRNA on the differentiation of these cells. (ac.ir)
Under the same neural differentiation-promoting conditions, the ability of each cell line to differentiate into neural progenitors varied. (biomedcentral.com)
The comparison of the unique properties and behavior of each individually derived cell line is critical in identifying the safe and efficacious lines for research and therapeutic use [ 3 , 13 ]. (biomedcentral.com)

Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine. (wikipedia.org)
The Centre for Stem Cell Biology and the Department of Biomedical Science, University of Sheffield, Western Bank, Sheffield S10 2TN, U.K. (silverchair.com)
As proof-of-concept, EmVP was applied to create complex synthetic biology-inspired intercellular communication models, where adipocyte differentiation is regulated by optogenetic-engineered pancreatic cells. (regenhu.com)

Regarding rescue tests, losartan (16 mg/kg in PBS) has been inserted intraperitoneally 30 LY3039478 Stem Cells & Wnt inhibitor minimum before nanoparticle administration. (parp1inhibitor.com)

Recently, the importance of telomere maintenance in human stem cells has been highlighted by studies on dyskeratosis congenital, which is a genetic disorder in the human telomerase component. (nature.com)
The regulation of telomere length and telomerase activity is a complex and dynamic process that is tightly linked to cell cycle regulation in human stem cells. (nature.com)
Here we review the role of telomeres and telomerase in the function and capacity of the human stem cells. (nature.com)

Abstract In living tissues, cells express their functions following complex signals from their surrounding microenvironment. (regenhu.com)

Growing viruses in cell cultures allowed preparation of purified viruses for the manufacture of vaccines . (wikipedia.org)
This vaccine was made possible by the cell culture research of John Franklin Enders , Thomas Huckle Weller , and Frederick Chapman Robbins , who were awarded a Nobel Prize for their discovery of a method of growing the virus in monkey kidney cell cultures. (wikipedia.org)
In contrast, glutamate release is enhanced in dense P19 neuronal cultures. (huji.ac.il)

As P19 cells have differentiation ability, they can be useful in investigating complex biological processes such as tissue formation and early embryonic development. (cytion.com)

In P19 cells, addition of non-toxic concentrations of drugs to aggregated embryoid body cells can induce differentiation of P19 cells into specific cell lines depending on the added drug. (wikipedia.org)
Methods Neuronal differentiation of P19 cells was initiated with 4-day RA treatment. (cams.cn)
Conclusions Acetylated p53 may participate in regulating the expression of map2 in RA-induced differentiation of P19 cells. (cams.cn)

Immunofluorescence, real-time reverse transcription-polymerase chain reaction (RT-PCR) assay, and map2 promoter driven luciferase assay were performed to detect the expression and relative promoter activity of MAP2 in those RA-treated cells. (cams.cn)

Tuning the mechanical and optical properties of gelatin-based microparticles enables their use as support bath for suspended extrusion printing, in which features containing high cell densities can be easily introduced. (regenhu.com)

ADAR1 silencing in breast cancer cells leads to increased apoptosis, suggesting an anti-apoptotic function that promotes cancer progression. (cancerindex.org)

Together, these results suggest that expression of PRTG defines a stage between pluripotent epiblasts and committed neural progenitors, and its signaling plays a critical role in suppressing premature neuronal differentiation during early neural development. (nycu.edu.tw)
We have also recently found that Cripto-1 can enhance canonical Wnt/beta-catenin signaling at limiting concentrations of Wnt by facilitating the binding of Wnt to the Lrp5 or Lrp6 co-receptors on the cell surface. (grantome.com)

Basically, given the difference of telomere and telomerase activity in human and mouse cells, the telomere and telomerase status in stem cell populations is different between humans and mice ( Harrington, 2004 ). (nature.com)

The map2 mRNA increased 34-fold after 4 days of RA treatment and 730-fold 2 days after the treatment, compared with the cells without RA treatment (control). (cams.cn)

In somatic cells, the activity of telomerase, a reverse transcriptase that can elongate telomeric repeats, is usually diminished after birth so that the telomere length is gradually shortened with cell divisions, and triggers cellular senescence. (nature.com)
Thus, even in stem cells, except for embryonal stem cells and cancer stem cells, telomere shortening occurs during replicative ageing, possibly at a slower rate than that in normal somatic cells. (nature.com)
Telomeric DNA consists of short guanine-rich repeat sequences in all eukaryotes with linear chromosomes, and its length in human somatic cells is remarkably heterogeneous among individuals ranging from 5 to 20 kb, according to age, organ, and the proliferative history of each cell ( Wright and Shay, 2005 ). (nature.com)
In most human somatic cells except for stem cells and lymphocytes, telomerase activity is diminished after birth so that telomere length shortens with each cell division. (nature.com)
The occurrence of highly polymorphic mono- and dinucleotide MICROSATELLITE REPEATS in somatic cells. (lookformedical.com)
A type of CELL NUCLEUS division by means of which the two daughter nuclei normally receive identical complements of the number of CHROMOSOMES of the somatic cells of the species. (lookformedical.com)

In the present study, a side-by-side comparison of the ability to maintain an undifferentiated state and to self-renew under standard conditions, the ability to spontaneously differentiate into cell types of three germ layers in embryonic bodies, and directed differentiation under neural differentiation-promoting conditions was made between three NIH registered hESC lines I3, I6 and BG01V. (biomedcentral.com)

J Cell Biol (2004) 166 (6): 853-863. (rupress.org)
J Cell Biol. (ac.ir)
Nat Rev Mol Cell Biol. (ac.ir)

Melatonin disturbs SUMOylation-mediated crosstalk between c-Myc and nestin via MT1 activation and promotes the sensitivity of paclitaxel in brain cancer stem cells. (cusabio.com)

We have previously utilised an NT2.D1 neurosphere based model of neuronal differentiation to study the effects of a variety of compounds on neural development [7] . (plos.org)

Anatomy8

Diseases5

Chemicals and Drugs1

Analytical, Diagnostic and Therapeutic Techniques and Equipment1

Phenomena and Processes1

Tumor19

Retinoic acid11

Murine3

Teratocarcinoma5

Mesenchymal stem3

Phenotype4

Glial7

Progenitor cells4

Tumors11

Induces2

Developmental5

Precursor3

Neurogenesis2

Cholinergic1

Ability to differentiate1

Astrocytes2

Epithelial3

Markers4

Neural stem2

Embryos3

Vitro4

Karyotype2

Neurons5

Medulloblastoma2

Pathways4

Chromosomal2

Mesoderm2

Communication and Signaling1

Malignant1

Precursors1

Characterization1

Gene5

Proteins5

Mammalian1

Germ cells1

Line18

Biology3

Inhibitor1

Human stem cells3

Abstract1

Cultures3

Embryonic development1

Differentiation of P19 cells3

Immunofluorescence1

High cell densities1

Apoptosis1

Signaling2

Populations1

MRNA1

Somatic cells6

Differentiate into cell types1

Biol3

Melatonin1

Neurosphere1