• Formalin-fixed cerebellar cortical tissue from four prospectively collected ET brains was sectioned and immunostained with a monoclonal phosphorylated neurofilament antibody (SMI-31, Covance, Emeryville, CA). Using additional sections from three ET brains, torpedoes were assessed using electron microscopy. (columbia.edu)
  • Chemically, these fibers are composed of glial fibrillary acidic protein and alpha-beta crystallin. (medscape.com)
  • To investigate the role of plasma neurofilament-light chain (NfL), glial fibrillary acidic protein (GFAP), total tau (t-tau) and ubiquitin C-terminal hydrolase L1(UCHL1) as biomarkers in FRDA. (frontiersin.org)
  • We compared the responses of neuronal processes immunoreactive for either neurofilament triplet (NF + ) or calretinin (CR + ) to fibrillar amyloid (Aβ) plaques in human end-stage and preclinical AD, as well as in APP/PS1 and Tg2576 transgenic mouse AD models. (frontiersin.org)
  • In this retrospective study, CSF concentrations of various brain-specific proteins were analyzed in patients with ALS (n = 32) and ALS-mimic disorders (n = 26). (nih.gov)
  • Using a synthetic KSPXXXK peptide to screen for a specific kinase, we fractionated rat brain extracts by column chromatography and identified extracellular signal-regulated kinase (Erk2) activated by an upstream activator, the mitogen-activated protein kinase kinase MAPKK (MEK), by Western blot analysis, sequence identification, and inhibition by a specific MEK inhibitor (PD 98059). (jneurosci.org)
  • Biochemical assembly studies have indicated that the NF68 protein forms the core of the filament and that the other two proteins are associated proteins. (duke.edu)
  • A mong cytoskeletal systems, the intermediate filaments (IFs) 1 are unique because they are composed of different proteins in different tissues. (rupress.org)
  • Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. (mybiosource.com)
  • NFL: one of the three (L, M, and H) intermediate filament proteins that form neurofilaments. (mybiosource.com)
  • To understand better the dynamics of intermediate filament formation, transport, and stability in a healthy, living cell , we inserted neurofilament heavy chain (NFH)-green fluorescent protein (GFP) fusion constructs in adenoviral vectors with tetracycline (tet)-regulated promoters. (nasa.gov)
  • Furthermore, the novel 4-plex assay appears to be a valid tool to simultaneously measure brain-derived proteins at extremely low concentrations in the peripheral circulation. (frontiersin.org)
  • Quantitative studies on aluminium deposition and its effects on neurofilament protein expression and phosphorylation, following the intraventricular administration of aluminium maltolate to adult rabbits. (thefreedictionary.com)
  • Although all three proteins have the tripartite structure described above, NF-M and NF-H have long, hypervariable COOH-terminal tails (NF-M 439 amino acids, NF-H 660 amino acids) containing several KSP repeats that are targets of phosphorylation. (rupress.org)
  • Neurofilament function depends on the state of phosphorylation of the numerous serine/threonine residues in these proteins. (jneurosci.org)
  • The MEK inhibitor PD 98059 also inhibited phosphorylation of NF-H, NF-M, and microtubule-associated protein (MAP) in primary rat hippocampal cells and caused a decrease in neurite outgrowth, suggesting that Erk1,2 may play an important role in neurite growth and branching. (jneurosci.org)
  • Identification of six phosphorylation sites in the COOH-terminal tail region of the rat neurofilament protein M". J. Biol. (wikipedia.org)
  • The peptide composition of Al-induced tangles is chiefly neurofilament protein whereas AD paired helical filaments are composed primarily of hyperphosphorylated tau (a microtubule associated protein) and ubiquitin (12,38,52). (thefreedictionary.com)
  • At this time, the most useful is the light subunit of the neurofilament protein (NFL). (chalmers.se)
  • Methods In a prospective study, we investigated CSF of 455 patients for neurofilament light chain (NfL), phosphorylated heavy chain (pNfH), tau protein (Tau) and phospho-tau protein (pTau). (diva-portal.org)
  • Here, we review what is known about the structure and function of neurofilaments, discuss analytical aspects and knowledge of age-dependent normal ranges of neurofilaments and provide a comprehensive overview of studies on neurofilament light chain as a marker of axonal injury in different neurological disorders, including multiple sclerosis, neurodegenerative dementia, stroke, traumatic brain injury, amyotrophic lateral sclerosis and Parkinson disease. (wikipedia.org)
  • Hereditary hypotrophic axonopathy with neurofilament deficiency in a mutant strain of Japanese quail' Acta Neuropathologica. (nii.ac.jp)