• Now Mossé and her colleagues at Children's Hospital are planning pediatric clinical trials of ALK inhibitors in children with high-risk neuroblastoma. (clpmag.com)
  • Less than 50% of children with high-risk neuroblastoma will survive following current multi-agent, aggressive therapy. (oncologynurseadvisor.com)
  • This finding will change the way we treat children with high-risk neuroblastoma in North America, which still claims many young lives and is in urgent need of better treatments," said Dr Park. (oncologynurseadvisor.com)
  • Integration of patient-derived models with patient data holds promise for the development of precision medicine treatment strategies for children with high-risk neuroblastoma. (lu.se)
  • is an oncogene that is overexpressed in approximately one quarter of cases of neuroblastoma via the amplification of the distal arm of chromosome 2. (medscape.com)
  • After detecting ALK mutations in familial neuroblastoma, the researchers then focused on the more common sporadic (non-familial) cases of neuroblastoma, and found that ALK mutations occurred in 12 percent of 194 tumor samples from the aggressive, high-risk form of the disease. (clpmag.com)
  • The American Cancer Society estimated that 710 new cases of neuroblastoma (including gangioneuroblastoma) would be diagnosed in the United States in 2014. (medscape.com)
  • Introduction of miR-193b mimics into nine neuroblastoma cell lines with distinct genetic characteristics significantly reduces cell growth in vitro independent of risk factors such as p53 functionality or MYCN amplification. (oncotarget.com)
  • Finally, the analysis of NK cells from patients with stage 4 NB suggests that NB conditioning could exert in vivo an immune modulatory effect resembling that emerged from in vitro experiments. (unicatt.it)
  • Imatinib mesylate has antitumor activity in vitro and in vivo against neuroblastoma cell lines and xenografts characterized by a different expression of receptor tyrosine kinases. (unicatt.it)
  • A GPC2-targeting antibody-drug conjugate was developed and was cytotoxic to neuroblastoma cells expressing GPC2 in vitro . (aacrjournals.org)
  • 3D scaffold-based in vitro cell culturing is a recent technological advancement in cancer research bridging the gap between conventional 2D culture and in vivo tumours. (rcsi.com)
  • Two neuroblastoma cell lines, chemotherapeutic sensitive Kelly and chemotherapeutic resistant KellyCis83 were cultured in a 3D in vitro model on two collagen-based scaffolds containing either glycosaminoglycan (Coll-GAG) or nanohydroxyapatite (Coll-nHA) and compared to 2D cell culture and an orthotopic murine model. (rcsi.com)
  • The efficacy of liposomes bearing miRNA mimics uptake and gene knockdown was similar in both 2D and 3D in vitro culturing models highlighting the proof-of-principle for the applicability of 3D collagen-based scaffolds cell system for validation of miRNA function. (rcsi.com)
  • STATEMENT OF SIGNIFICANCE: Traditional 2D cell cultures do not completely capture the 3D architecture of cells and extracellular matrix contributing to a gap in our understanding of mammalian biology at the tissue level and may explain some of the discrepancies between in vitro and in vivo results. (rcsi.com)
  • In the cell-free in vitro electron spin resonance (ESR) spectrometry, DA possessing two hydroxyl groups showed generation of DA-semiquinone radicals, which were markedly prevented by addition of SOD or GSH but not by catalase. (elsevierpure.com)
  • Here we review recent developments in patient-derived neuroblastoma xenograft models and in vitro cultures. (lu.se)
  • These persons total, had been reported in New York City (NYC) methods included an in vitro mouse neuroblastoma cell assay during the preceding 10 years (2000-2009). (cdc.gov)
  • The molecular basis of tumor metastasis and current approaches to decode targeted migration-promoting events in pediatric neuroblastoma. (harvard.edu)
  • To define core regulatory circuitries (CRCs) controlling the gene expression program of neuroblastoma, we established and analyzed the neuroblastoma super-enhancer landscape. (nih.gov)
  • He also is a member of the Center for Advanced Innate Cell Therapy at Texas Children's Cancer Center, the Center for Cell and Gene Therapy at Baylor and Texas Children's and the Dan L Duncan Comprehensive Cancer Center at Baylor. (ecancer.org)
  • Another important finding revealed a regulatory gene in NKT cells that may impact the efficacy of treatment. (ecancer.org)
  • Leveraging the multiomic platform of key collaborator Immunai, Inc., Baylor researchers found that upregulation of the BTG anti-proliferation factor 1 (BTG1) gene in infused CAR NKT cells is indicative of cell exhaustion and restricts CAR NKT cell functional activity. (ecancer.org)
  • Marked upregulation of the metastasis suppressor N-myc downstream regulated gene-1 (NDRG1), which is known to be activated and upregulated by thiosemicarbazones in adult cancers, was also detected in thiosemicarbazone-treated neuroblastoma cells. (muni.cz)
  • It was the third RAS gene to be discovered, and was named NRAS, for its initial identification in human neuroblastoma cells. (wikipedia.org)
  • Scientists have discovered gene mutations that are the main cause of the inherited version of the childhood cancer neuroblastoma. (clpmag.com)
  • Furthermore, because there already are drugs in development that target the same gene in adult cancers, we can soon begin testing those drugs in children with neuroblastoma. (clpmag.com)
  • Further sequencing of that region identified mutations in the anaplastic lymphoma kinase (ALK) gene in eight families with familial neuroblastoma. (clpmag.com)
  • The CAR-NKT therapy can be enhanced by disrupting a gene that promotes neuroblastoma growth, improving antitumor responses in preclinical trials. (cancer.org)
  • Researchers believe the deleted region could contain a gene that keeps cells from growing and dividing too quickly or in an uncontrolled way, called a tumor suppressor gene. (medlineplus.gov)
  • In this study, gene expression of 19,723 cancer cells was analysed and compared to a reference of 57,972 developmental adrenal cells in the hope of identifying the cell types from which neuroblastomas arise and to find novel treatment targets. (sanger.ac.uk)
  • But in recent years, technological advances have sped up drug development by allowing researchers to identify differences between the biological processes, such as the expression of a particular gene, within healthy human cells and those within cancerous ones. (sanger.ac.uk)
  • Here, the protective activity of emodin inhibiting zinc-induced neurotoxicity and its molecular mechanisms such as cellular Zn 2+ influx and zinc-induced gene expression were examined using human neuroblastoma cells (SH-SY5Y cells). (silverchair.com)
  • A gene mutation causes this cancer to form in the nerve cells. (webmd.com)
  • Since genes are composed of a given alphabet (A, T, C, G) with fixed pairing properties A-T and C-G, probes can be constructed that attract gene transcripts extracted from cell tissues and cultures. (lu.se)
  • DNA fragmentation, caspase-3 activation, and apoptosis-related gene expression were studied in CATH.a cells. (cdc.gov)
  • Our work provides a new and precious data resource to better understand the neuroblastoma ecosystem and suggest novel therapeutic strategies, targeting both tumor cells and components of the microenvironment. (curie.fr)
  • Conversely, engineered reduction of BTG1 expression in CAR NKT cells enhanced their therapeutic activity against neuroblastoma in a mouse model. (ecancer.org)
  • Being able to induce this differentiation by not leaving it to chance would be a therapeutic challage and could categorically change the prognosis of neuroblastomas. (lupinepublishers.com)
  • Activating mutations in druggable targets are rare in neuroblastoma, supporting investigation of other therapeutic strategies. (aacrjournals.org)
  • GPC2 was highly expressed on the plasma membrane of the majority of neuroblastoma tumors, and its expression was limited in normal tissues, suggesting the possibility for therapeutic targeting with minimal toxicity. (aacrjournals.org)
  • The dependency of neuroblastoma cells on GPC2 expression may prevent the emergence of immune escape mechanisms in response to therapeutic targeting. (aacrjournals.org)
  • The aim of this study was to establish a physiologically relevant 3D neuroblastoma tissue-engineered system and explore its therapeutic relevance. (rcsi.com)
  • There is thus a need for clinically relevant and authentic models of neuroblastoma that closely resemble the human disease to further interrogate underlying mechanisms and to develop novel therapeutic strategies. (lu.se)
  • Researchers at Baylor College of Medicine/Texas Children's Cancer Center and collaborating institutions report interim results from a first-in-human phase 1 clinical trial evaluating the safety, antitumor activity and immunological characteristics of a genetically engineered natural killer T (NKT) cell immunotherapy for neuroblastoma, a childhood tumour that most commonly arises in the adrenal gland. (ecancer.org)
  • Can T-Cell Immunotherapy Work in Osteosarcoma and Neuroblastoma? (solvingkidscancer.org)
  • A study in Nature suggests epitope editing in donor stem cells prior to bone marrow transplants can stave off toxicity when targeting acute myeloid leukemia with immunotherapy. (genomeweb.com)
  • Identification and targeting of protein tyrosine kinase 7 (PTK7) as an immunotherapy candidate for neuroblastoma. (harvard.edu)
  • Collectively, these findings suggest that GPC2 may be an optimal target for immunotherapy in high-risk neuroblastoma and other embryonal tumors, supporting further development of GPC2-directed immunotherapeutics. (aacrjournals.org)
  • By focusing on an alternative strategy to treating neuroblastoma tumors, we identified a compound class that in early testing in neuroblastoma cells in the laboratory shows promise for treating children with this disease. (sciencedaily.com)
  • The sternal puncture showed a very poor marrow with the presence of rarely isolated malignant cells often grouped in rosettes evoking an invasion by a neuroblastoma. (lupinepublishers.com)
  • The 5hmC profiles revealed distinct signatures characteristic to different cell lineages and stages of malignant transformation of NB cells in a conventional and oxygen-depleted environment, which often occurs in tumors. (figshare.com)
  • Neuroblastomas are immature, undifferentiated, malignant tumors. (msdmanuals.com)
  • In 1927, Cushing and Wolbach further characterized neuroblastoma by describing the transformation of malignant neuroblastoma into its benign counterpart, ganglioneuroma . (medscape.com)
  • Neuroblastoma is a malignant neural tumor that typically affects very young children. (medscape.com)
  • Malignant transformation and maintenance of the dedifferentiated state of neural crest cells may result from failure of those cells to respond to normal signals that are responsible for normal morphologic differentiation. (medscape.com)
  • Functional studies demonstrated dependency of neuroblastoma with noradrenergic identity on PHOX2B, evocative of lineage addiction. (nih.gov)
  • Less than half of these patients are cured, even with the use of high-dose therapy followed by autologous bone marrow or stem cell rescue. (medscape.com)
  • Altogether our data propose a novel tumor escape-mechanism based on the modulation of chemokine receptors that play pivotal roles in NK cells bone marrow homing, egress, or recruitment into peripheral tissues. (unicatt.it)
  • Neuroblastoma is a tumor of the peripheral sympathetic nervous system, derived from multipotent neural crest cells (NCCs). (nih.gov)
  • In the present studies, we examined the expression of two PDGF receptor genes in human tumor cell lines derived from neural crest. (jci.org)
  • The expression of alpha and/or beta PDGF receptors was detected in a wide variety of neural crest-derived human tumor cell lines such as neuroblastoma, primitive neuroectodermal tumor, and Ewing's sarcoma by RNA blot analysis, and confirmed by immunoblot analysis. (jci.org)
  • A common neoplasm of early childhood arising from neural crest cells in the sympathetic nervous system, and characterized by diverse clinical behavior, ranging from spontaneous remission to rapid metastatic progression and death. (harvard.edu)
  • Neuroblastoma is a tumor of neural crest origin that arises in tissue of sympathetic nervous system and occurs in young children," said lead investigator Julie R. Park, MD, professor of medicine at Seattle Children's Hospital and the University of Washington. (oncologynurseadvisor.com)
  • Neuroblastoma arises from neural crest progenitor cells in the developing sympathetic nervous system, and thus expresses targets not found on mature cells, suggesting the possibility for the development of immunotherapies. (aacrjournals.org)
  • [ 1 ] It is a poorly differentiated neoplasm derived from neural crest cells that typically affects infants and young children. (medscape.com)
  • All of these tumors arise from primordial neural crest cells, which ultimately populate the sympathetic chain and the adrenal medulla. (medscape.com)
  • Features of related cells were validated by multicolor flow cytometry and functional assays.ResultsWe show that the immune microenvironment of MYCN-driven mouse neuroblastoma is characterized by a low content of T cells, several phenotypes of macrophages and a population of cells expressing signatures of myeloid-derived suppressor cells (MDSCs) that are molecularly distinct from the various macrophage subsets. (curie.fr)
  • While past research has pointed to a role for circularized, extrachromosomal MYCN oncogene sequences in neuroblastoma, the team explained, the full suite and the frequency of somatic mutations involving small or large stretches of circularized extrachromosomal DNA amplifications had not been fully explored. (genomeweb.com)
  • Even so, the team's follow-up analyses - including RNA sequencing experiments - indicated that rearrangements stemming from extrachromosomal circular DNA from MYCN and other genes may be a recurrent and ongoing source of new mutations through a multi-hit model in neuroblastoma. (genomeweb.com)
  • Functionally, miR-193b induces a G1 cell cycle arrest and cell death in neuroblastoma cell lines by reducing the expression of MYCN , Cyclin D1 and MCL-1 , three important oncogenes in neuroblastoma of which inhibition has shown promising results in preclinical testing. (oncotarget.com)
  • MYCN bound to the GPC2 promoter to increase its expression in neuroblastoma, thereby upregulating GPC2 in MYCN -amplified tumors. (aacrjournals.org)
  • Further, the antibody-drug conjugate promoted durable tumor regression in a patient-derived xenograft (PDX) model of MYCN -amplified neuroblastoma, extending survival with minimal toxicity. (aacrjournals.org)
  • MYCN amplification occurs in about 20% of neuroblastoma cases and is associated with advanced disease and unfavorable biology. (msdmanuals.com)
  • With that in mind, Henssen and colleagues from Germany, the US, and Spain profiled matched tumor and normal blood samples from 93 neuroblastoma patients using whole-genome sequencing and an algorithm that uncovers circularized DNA based on paired read orientation, uncovering preliminary evidence for complex and relatively frequent ecDNAs in neuroblastoma. (genomeweb.com)
  • The scientists analyzed neuroblastoma tissue samples from 93 children. (mskcc.org)
  • [ 8 ] In 1957, Mason published a report of a child with neuroblastoma whose urine contained pressor amines. (medscape.com)
  • Asymptomatic reactivation of SARS-CoV-2 in a child with neuroblastoma characterised by whole genome sequencing. (cdc.gov)
  • The presence of sympathoblasts, a developmental cell type not normally found in children after they are born, makes it a promising drug target for the treatment of neuroblastoma. (sanger.ac.uk)
  • Neuroblastoma (NB) is a pediatric cancer of the developing sympathetic nervous system that exhibits significant variation in the stage of differentiation and cell composition of tumors. (figshare.com)
  • We therefore examined the roles of oxidative stress and proteasomal inhibition on protein aggregates induction in naïve and neuronally differentiated neuroblastoma SH-SY5Y cells. (unboundmedicine.com)
  • Naïve and transfected cells were exposed to oxidative stress induced by rotenone, SIN-I, FeCl(2,) and to proteasomal inhibition by lactacystin. (unboundmedicine.com)
  • Although studies in normal neuronal cells would be useful to further confirm our hypothesis, we suggest that specific PEMF treatments may have potential biological repercussions in diseases where oxidative stress is implicated. (nih.gov)
  • The accumulation of cytosolic lipid globules and oxidative stress finally correlates with the apoptotic death of cells . (bvsalud.org)
  • In conclusion, DA content was not responsible for the enhanced sensitivity of CATH.a cells to Mn toxicity, but oxidative stress was implicated as a probable mechanism of cytotoxicity. (cdc.gov)
  • Tumor microenvironment represents a key component of the tumor ecosystem the complexity of which has to be accurately understood to define selective targeting opportunities, including immune-based therapies.MethodsWe combined various approaches including single-cell transcriptomics to dissect the tumor microenvironment of both a transgenic mouse neuroblastoma model and a cohort of 10 biopsies from neuroblastoma patients, either at diagnosis or at relapse. (curie.fr)
  • We further functionally demonstrate that MDSCs isolated from mouse neuroblastoma have immunosuppressive properties, impairing the proliferation of T lymphocytes.ConclusionsOur study demonstrates that neuroblastoma tumors have an immunocompromised microenvironment characterized by dysfunctional T cells and accumulation of immunosuppressive cells. (curie.fr)
  • Simultaneous administration of the drugs consistently resulted in synergistic and/or additive activities against the cell lines derived from the most frequent types of pediatric solid tumors. (muni.cz)
  • This trial studies the side effects and best dose of activated T cells armed with GD2 bispecific antibody and how well they work in treating patients with neurblastoma, osteosarcoma, and other GD2+ solid tumors. (solvingkidscancer.org)
  • Of the solid tumors of childhood, neuroblastoma-the prototypic small-, round-, blue-cell neoplasm-occurs in the youngest patients and has shown the least predictable biologic behavior and response to therapy. (johnshopkins.edu)
  • Human neuroblastoma cells express alpha and beta platelet-derived growth factor receptors coupling with neurotrophic and chemotactic signaling. (jci.org)
  • We have also demonstrated that PDGF receptors on the human neuroblastoma cell lines were biologically functional. (jci.org)
  • These findings suggest that PDGF isoforms are involved not only in the promotion of the neuroblastoma cell growth, but also in neuronal cell migration, growth, and differentiation in human brain development. (jci.org)
  • We found that a 40 min overall (four times a week, 10 min each) pre-exposure to PEMF did not affect major physiological parameters and led to a significant increase of Mn-dependent superoxide dismutase activity in the human neuroblastoma SH-SY5Y cell line. (nih.gov)
  • Our data unravel a complex content in myeloid cells in patient tumors and further document a striking correspondence of the microenvironment populations between both mouse and human tumors. (curie.fr)
  • In summary, the present data could be of potential relevance to identify the mechanisms of action by which extremely low frequency magnetic fields affect human cells . (emf-portal.org)
  • Mutations which change amino acid residues 12, 13 or 61 activate the potential of N-ras to transform cultured cells and are implicated in a variety of human tumors e.g. melanoma. (wikipedia.org)
  • 1. In this study we have investigated δ and μ opioid receptor-mediated elevation of intracellular Ca 2+ concentration ([Ca 2+ ](i)) in the human neuroblastoma cell line, SH-SY5Y. (edu.au)
  • Neuroblastoma is one of the few human malignancies known to demonstrate spontaneous regression from an undifferentiated state to a completely benign cellular appearance. (medicalxpress.com)
  • Genetic Clinical Markers of Human Neuroblastoma with Special Reference to N- myc Oncogene: Amplified or Not Amplified? (karger.com)
  • The incidence of spontaneous regression in neuroblastoma is estimated to be between 10 and 100 times greater than that of any other human cancer [3]. (lupinepublishers.com)
  • We also generated genome-wide maps of PARIS occupancy using ChIP-seq in human SH-SY5Y cells. (nature.com)
  • Chromosome 1 is the largest human chromosome, spanning about 249 million DNA building blocks (base pairs) and representing approximately 8 percent of the total DNA in cells. (medlineplus.gov)
  • The project aims to create comprehensive reference maps of all types of human cells - the fundamental units of life - as a basis for understanding human health and diagnosing, monitoring, and treating disease. (sanger.ac.uk)
  • The incorporation and redistribution of [1-14C]arachidonic acid in SK-N-BE human neuroblastoma cell phospholipids were investigated. (torvergata.it)
  • In this study, we show that neuroblastoma (NB) cell conditioning affects the chemokine receptor repertoire of human resting NK cells. (unicatt.it)
  • The objective of the experiment was to test the effects of various herbal extracts (bugleweed, hops, sassafras, and white camphor) on Aβ 1-40 peptide levels in human neuroblastoma cells that were transfected to overexpress APP. (emerginginvestigators.org)
  • Neurotoxic properties of L-dopa and dopamine (DA)-related compounds were assessed in human neuroblastoma SH-SY5Y cells with reference to their structural relationship. (elsevierpure.com)
  • Mefloquine binding to human acyl-CoA binding protein leads to redox stress-mediated apoptotic death of human neuroblastoma cells. (bvsalud.org)
  • In this study, we show that mefloquine binds to and inactivates the human acyl-CoA binding protein ( hACBP ), potentially inducing redox stress in human neuroblastoma cells (IMR-32). (bvsalud.org)
  • Taken together, our study deciphers a mechanistic detail of how mefloquine leads to the death of human cells by perturbing the activity of hACBP and lipid homeostasis . (bvsalud.org)
  • In the present study, Mn toxicity was characterized in a human neuroblastoma (SK-N-SH) cell line and in a mouse catecholaminergic (CATH.a) cell line. (cdc.gov)
  • Transcriptional landscape of human neuroblastoma cells in response to SARS-CoV-2. (cdc.gov)
  • Additionally, researchers found a correlation between higher antitumor activity and in vivo proliferation of infused CAR-NKT cells. (ecancer.org)
  • To test whether a magnetic field of 10 µT and 100 µT influences the proliferation of a neuroblastoma cell line , alone or in combination with retinoic acid (a retinoid applied in oncostatic therapies). (emf-portal.org)
  • The experiments showed that exposure to magnetic fields of 10 µT or 100 µT significantly increased the proliferation and the DNA content when compared to the control samples, but did not influence the cell viability and the total protein content. (emf-portal.org)
  • However, the second experiment showed that a treatment with retinoic acid significantly reduced the proliferation in comparison to the control cell culture , independent if the cell cultures were exposed or sham exposed . (emf-portal.org)
  • The authors conclude that a magnetic field exposure of 10 µT or 100 µT could enhance the cell proliferation in a neuroblastoma cell line and that retinoic acid may inhibit this effect. (emf-portal.org)
  • Moreover, GPC2 was required for neuroblastoma cell proliferation, and its depletion resulted in apoptosis. (aacrjournals.org)
  • Our results reveal cooperation between individual miR-17-92 miRNAs and implicate miR-17-92 in multiple hallmarks of cancer, including proliferation and cell adhesion. (lu.se)
  • Neuroblastoma is one of the small, blue, round cell tumors of childhood. (medscape.com)
  • Beyond the standard approach of using drugs that kill tumor cells, another promising strategy is to identify compounds that promote differentiation, which causes tumor cells to stop dividing and growing. (sciencedaily.com)
  • To address this need, Stegmaier and her team developed a method to screen small molecules for their ability to trigger differentiation in neuroblastoma cells. (sciencedaily.com)
  • First, they treated these cells with drugs known to induce differentiation and looked for accompanying changes in the activity levels of genes. (sciencedaily.com)
  • Using this genetic signature for differentiation, they then screened nearly 2,000 small molecules and identified one compound that strongly promoted differentiation in neuroblastoma cells, especially when combined with a drug already approved to treat this type of cancer. (sciencedaily.com)
  • Originally discovered as a chief regulatory protein of glucose and lipid metabolism and cell differentiation, the peroxisome proliferator-activated receptor gamma (PPARγ) is a ligand-activated transcription factor of the nuclear hormone receptor superfamily 8 . (nature.com)
  • Glial cell line-derived neurotrophic factor family receptor alpha (GFRα) members have been widely connected to the mechanisms contributing to cell growth, differentiation, cell migration and tissue maturation. (medsci.org)
  • The glial cell line-derived neurotrophic factors (GDNFs), a family of neurotrophic factors, were initially thought to be able to regulate the growth, survival, and differentiation of neural-derived cell types. (medsci.org)
  • Based on whether it cooperates with the second receptor RET, GFRα has also been widely linked to the mechanisms that contribute to cell growth, differentiation and migration and tissue maturation. (medsci.org)
  • This inspires hope that novel immunotherapeutic strategies, such as the one studied in this trial, will ultimately improve the outcomes for children with neuroblastoma," Metelitsa said. (ecancer.org)
  • Neuroblastoma has long been a puzzling disease, partly because of its broad range in outcomes. (clpmag.com)
  • Neurocognitive outcomes in adult survivors of neuroblastoma: A report from the Childhood Cancer Survivor Study. (harvard.edu)
  • Because pilot studies of tandem autologous HCT demonstrated tolerable toxicity and potential efficacy and because prior trials have demonstrated that dose intensification improves outcomes, researchers decided to explore tandem transplantation in patients with high-risk neuroblastoma. (oncologynurseadvisor.com)
  • Neuroblastoma is an aggressive pediatric cancer that develops from early nerve cells and accounts for up to 10% of childhood cancer deaths. (medicalxpress.com)
  • Researchers at Lund University in Sweden have identified one of the reasons why the childhood cancer neuroblastoma becomes resistant to chemotherapy. (medicalxpress.com)
  • John M Maris, MD, senior author of the current study and director of the Center for Childhood Cancer Research at Children's Hospital, leads a laboratory with the world's largest collection of neuroblastoma tissue samples, gathered through the multicenter Children's Oncology Group in the US and through multiple international collaborations. (clpmag.com)
  • The largest single cell study to date of the childhood cancer, neuroblastoma, has answered important questions about the genesis of the disease. (sanger.ac.uk)
  • Neuroblastoma is an unusual cancer in that some tumours resolve without intervention, yet the disease still has one of the lowest five-year survival rates of any childhood cancer. (sanger.ac.uk)
  • Our study shows the power of looking at individual childhood cancer cells in revealing not just one, but a plethora of novel treatment ideas. (sanger.ac.uk)
  • This raises the exciting prospect that a single cell atlas of all types of paediatric tumours may transform our understanding of childhood cancer. (sanger.ac.uk)
  • Neuroblastoma is a childhood cancer derived from the sympathetic nervous system. (lu.se)
  • High-risk neuroblastoma patients have a poor overall survival and account for ~15% of childhood cancer deaths. (lu.se)
  • In this study, we found the CD62L biomarker expressed on the infused NKT cells, which we identified in our preclinical work, is predictive of both higher NKT cell in vivo expansion and antitumor activity in patients. (ecancer.org)
  • This trial wil also provide a preliminary assessment of the effect of these NKT cells on high risk neuroblastoma and the duration of time that the cells last in the body. (texaschildrens.org)
  • Nearly two-thirds of patients with high-risk neuroblastoma -- a common tumor that forms in the nerve cells of children -- cannot be cured using tumor-killing cancer drugs. (sciencedaily.com)
  • The survival rate of patients with high-risk neuroblastoma is still very low despite intensive multimodal treatments. (oncotarget.com)
  • A disease exhibiting extreme heterogeneity, neuroblastoma is stratified into three risk categories: low, intermediate, and high risk. (medicalxpress.com)
  • This study is looking at chemotherapy, radiotherapy and stem cell transplants for people with high risk neuroblastoma. (cancerresearchuk.org)
  • You may be able to join this study if have high risk neuroblastoma that has spread to nearby tissue or to another part of the body. (cancerresearchuk.org)
  • In addition, the researchers found that the same mutations play a significant role in high-risk forms of non-inherited neuroblastoma, the more common form of the disease. (clpmag.com)
  • Targeting macrophage Syk enhances responses to immune checkpoint blockade and radiotherapy in high-risk neuroblastoma. (harvard.edu)
  • Impact of diagnostic and end-of-induction Curie scores with tandem high-dose chemotherapy and autologous transplants for metastatic high-risk neuroblastoma: A report from the Children's Oncology Group. (harvard.edu)
  • For the study, researchers enrolled 355 children (median age, approximately 3 years) who were newly diagnosed with high-risk neuroblastoma. (oncologynurseadvisor.com)
  • However, the regimen we use for high-risk neuroblastoma is also the most aggressive and toxic regimen we give to children with cancer. (oncologynurseadvisor.com)
  • There is strong rationale to further explore intensified consolidation in high-risk neuroblastoma," Dr Valteau-Couanet said. (oncologynurseadvisor.com)
  • A GPC2-targeting antibody-drug conjugate promotes tumor regression in a high-risk neuroblastoma PDX. (aacrjournals.org)
  • Immunotherapeutic GPC2 targeting may be beneficial in high-risk neuroblastoma and other embryonal tumors. (aacrjournals.org)
  • Using an RNA-sequencing (RNA-seq)-based pipeline to analyze 126 primary high-risk neuroblastomas, Bosse and colleagues identified candidate genes for optimal immunotherapeutic targeting. (aacrjournals.org)
  • Further, in approximately 40% of high-risk neuroblastomas somatic gain of chromosome 7q, which harbors the GPC2 locus, promoted enhanced GPC2 expression. (aacrjournals.org)
  • Identification of GPC2 as an oncoprotein and candidate immunotherapeutic target in high-risk neuroblastoma. (aacrjournals.org)
  • Bottom right panel, ganglioneuroma: Mature ganglion cell with schwannian stroma. (medscape.com)
  • The specificity of our case is the benign evolution of an osteomedullary stage 4 neuroblastoma and its maturation into a ganglioneuroma 6 years after the end of chemotherapy. (lupinepublishers.com)
  • In addition, the evolution of neuroblastoma is unlikely: the tumor can be aggressive and evolves rapidly into a metastatic disease, as it can be "nice" and undergoes regression or maturation (spontaneous or probably induced) into a benign tumor: the ganglioneuroma. (lupinepublishers.com)
  • As we increase our knowledge of ALK mutations, we will also offer specialized diagnostic testing for all newly diagnosed patients with neuroblastoma, to eventually allow oncologists to better customize treatment to a child's genetic profile. (clpmag.com)
  • Survival of Patients With Neuroblastoma After Assignment to Reduced Therapy Because of the 12- to 18-Month Change in Age Cutoff in Children's Oncology Group Risk Stratification. (harvard.edu)
  • Close collaborating between COG (Children's Oncology Group) and SIOPEN (International Society of Paediatric Oncology Europe Neuroblastoma) will allow for the design of complementary studies to define the most effective risk-tailored intensified strategies. (oncologynurseadvisor.com)
  • Allogeneic hematopoietic cell transplantation for neuroblastoma: the CIBMTR experience. (uchicago.edu)
  • NEW YORK - New research suggests circularized DNA falling outside of linear chromosomes may serve as a recurrent source of somatic rearrangements in neuroblastoma, a pediatric cancer affecting immature cells in the sympathetic nervous system. (genomeweb.com)
  • These deletions are somatic mutations, which means they occur during a person's lifetime and are present only in the cells that become cancerous. (medlineplus.gov)
  • These Ras genes have GTP/GDP binding and GTPase activity, and their normal function may be as G-like regulatory proteins involved in the normal control of cell growth. (wikipedia.org)
  • We investigated by real-time quantitative RT-PCR the expression profile of a panel of genes important in cell cycle regulation. (biomedcentral.com)
  • Researchers have identified several possible tumor suppressor genes in the deleted region of chromosome 1, and more research is needed to understand what role these genes play in neuroblastoma development. (medlineplus.gov)
  • This was made possible by the advent of single cell mRNA sequencing, a high-resolution technology that can identify different cell types present in a tissue according to the genes expressed by individual cells. (sanger.ac.uk)
  • Our scientists pursue every aspect of cancer research-from exploring the biology of genes and cells, to developing immune-based treatments, uncovering the causes of metastasis, and more. (mskcc.org)
  • cDNA hydridization array analysis with two differing Mn concentrations and time points, identified no noteworthy mRNA inductions of genes associated with programmed cell death. (cdc.gov)
  • In addition, we found PEMF-pre-exposed cells exhibited decreased reactive oxygen species production following a 30 min H2 O2 challenge, with respect to non pre-exposed cells. (nih.gov)
  • NKT cells were modified to express a GD2-specific chimeric antigen receptor (GD2 CAR), which enables the immune cells to target a molecule found on the surface of neuroblastoma cells, and interleukin-15 (IL-15), a natural protein that supports NKT cell survival. (ecancer.org)
  • We have also demonstrated that NKTs destroy tumor-associated macrophages (TAMs), which are cells that support the growth and survival of NBs. (texaschildrens.org)
  • This important molecule promotes NKT cell survival and function within tumor tissues. (texaschildrens.org)
  • This study will provide new information on whether infusions of these activated T-cells will not only kill the tumor, but also "vaccinate" the patients against their own cancer resulting in significant improvement in survival for patients with relapsed GD2-positive tumors. (solvingkidscancer.org)
  • Children who suffer a relapse of the aggressive cancer known as neuroblastoma have small chances of survival. (medicalxpress.com)
  • The study, published today (5 February 2021) in Science Advances , sought to understand why neuroblastomas range in severity, with some easy to treat and others having relatively low five-year survival rates. (sanger.ac.uk)
  • Neuroblastoma (NB) is a deadly childhood disease that carries a 50% chance of relapse for anyone in remission and similar level of 5-year survival. (psu.edu)
  • Neuroblastoma is a type of cancerous tumor composed of immature nerve cells (neuroblasts). (medlineplus.gov)
  • Neuroblastoma develops in immature nerve cells and is the most common form of cancer in infants. (mskcc.org)
  • BackgroundHigh-risk neuroblastoma is a pediatric cancer with still a dismal prognosis, despite multimodal and intensive therapies. (curie.fr)
  • Patients with low-risk and intermediate-risk neuroblastoma have excellent prognosis and outcome. (medscape.com)
  • Deletion of the short arm of chromosome 1 is the most common chromosomal abnormality present in neuroblastoma and confers a poor prognosis. (medscape.com)
  • Iron is an essential cellular nutrient, being a critical cofactor of several proteins involved in cell growth and replication. (aspetjournals.org)
  • In addition, proteome analysis of NBPF1-overexpressing DLD1 cells identified 32 differentially expressed proteins, of which several are implicated in carcinogenesis. (biomedcentral.com)
  • We demonstrated that NBPF1 exerts different tumor suppressive effects, depending on the cell line analyzed, and provide new clues into the molecular mechanism of the enigmatic NBPF proteins. (biomedcentral.com)
  • The putative targets were cell-surface proteins (or proteins with extracellular epitopes) highly expressed in neuroblastoma compared with normal tissues, and expressed in the majority of tumors. (aacrjournals.org)
  • Analysis of SARS-CoV-2 infection associated cell entry proteins ACE2, CD147, PPIA, and PPIB in datasets from non SARS-CoV-2 infected neuroblastoma patients, as potential prognostic and infection biomarkers in neuroblastoma. (cdc.gov)
  • Li Q, Cao Z, Zhao S. The Emerging Portrait of Glial Cell Line-derived Neurotrophic Factor Family Receptor Alpha (GFRα) in Cancers. (medsci.org)
  • It also has orphan designations for treating various cancers, including neuroblastoma . (medscape.com)
  • [ 1 , 2 ] Neuroblastoma is the third most common malignancy in children up through 14 years of age, behind acute lymphocytic leukemia and cancers of the brain and central nervous system. (medscape.com)
  • Given the documented sensitivity of NB cells to HSP90 inhibition, we propose CPX and other iron chelators as investigational antitumor agents in NB therapy. (aspetjournals.org)
  • This study provides promising initial evidence of antitumor activity of GD2 CAR NKT cells against neuroblastoma. (ecancer.org)
  • This further suggests that in neuroblastoma the antitumor activity of imatinib may involve the inhibition of other tyrosine kinases and/or pathways. (unicatt.it)
  • We show that mouse intratumor T cells exhibit increased expression of inhibitory receptors at the protein level. (curie.fr)
  • Strikingly, emodin elevated SH-SY5Y cell viability and inhibited cell apoptosis caused by AMP-activated protein kinase signaling pathway activation. (silverchair.com)
  • In this study, we have investigated whether alpha/beta IFN produced by NS20Y/MS cells activates cellular protein tyrosine kinases which will induce tyrosine phosphorylating activity specific to virus-infected cells. (bgu.ac.il)
  • We present data to show augmented protein tyrosine kinase activity in the persistently infected cells. (bgu.ac.il)
  • We demonstrate that the MV N protein is phosphorylated on tyrosine in addition to serine and threonine in the persistent state but not in NS20Y cells acutely infected with MV. (bgu.ac.il)
  • Using SILAC and quantitative mass spectrometry, we examined the effects of activation of the miR-17-92 cluster on global protein expression in neuroblastoma (NB) cells. (lu.se)
  • Chromogranin A is a secretory protein, composed of 439 amino acids, found in the large dense-core vesicles of the neuroendocrine cells. (medscape.com)
  • Chromogranin A (CgA) is a secretory protein, composed of 439 amino acids, found in the large dense-core vesicles of the neuroendocrine cells. (medscape.com)
  • Neuroblastoma is a cancer arising in the adrenal gland or less often from the extra-adrenal sympathetic chain, including in the retroperitoneum, chest, and neck. (msdmanuals.com)
  • In the new article, investigators describe the results obtained in 12 patients with stage 4 relapsed neuroblastoma that was resistant to other therapies. (ecancer.org)
  • Since the mutations discovered by Mossé trigger an "on" signal for neuroblastoma cells, the abnormality is an outstanding target for therapies that inhibit the ALK protein's activity. (clpmag.com)
  • My hope is that new, less intrusive therapies can be developed by targeting sympathoblasts, a developmental cell type that exists only in neuroblastoma tumours after a child is born. (sanger.ac.uk)
  • Collectively, this data shows the successful development and characterisation of a physiologically relevant, scaffold-based 3D tissue-engineered neuroblastoma cell model, strongly supporting its value in the evaluation of chemotherapeutics, targeted therapies and investigation of neuroblastoma pathogenesis. (rcsi.com)
  • This GINAKIT2 phase 1 clinical trial, the first to use of genetically engineered NKT cells in humans, will evaluate the anti-tumor activity of GD2 specific CAR and interleukin-15 expressing autologous NKT cells. (texaschildrens.org)
  • Humans normally have 46 chromosomes in each cell, divided into 23 pairs. (medlineplus.gov)
  • To understand the mechanisms underlying MV persistence in neuronal cells, a tissue culture model was studied. (bgu.ac.il)
  • CgA is co-stored with a tissue-specific peptide hormone or neuropeptide and exerts an autocrine and paracrine effect on secretory activity of the neuroendocrine cells. (medscape.com)
  • A physiologically relevant 3D collagen-based scaffold-neuroblastoma cell system exhibits chemosensitivity similar to orthotopic xenograft models. (rcsi.com)
  • Both neuroblastoma cell lines actively infiltrated the scaffolds and proliferated displaying >100-fold increased resistance to cisplatin treatment when compared to 2D cultures, exhibiting chemosensitivity similar to orthotopic xenograft in vivo models. (rcsi.com)
  • Effects on cell viability of one of the compounds, CPX, were indeed dependent on chelation of intracellular iron and mediated by both G 0 /G 1 cell cycle block and induction of apoptosis. (aspetjournals.org)
  • The results of a detailed analysis of cell signaling in the neuroblastoma cell lines revealed that TKIs inhibited the phosphorylation of the corresponding receptor tyrosine kinases, and thiosemicarbazones downregulated the expression of epidermal growth factor receptor, platelet-derived growth factor receptor, and insulin-like growth factor-1 receptor, leading to a strong induction of apoptosis. (muni.cz)
  • Our findings showed that emodin obviously enhanced cell viability and reduced cell apoptosis and lactate dehydrogenase release. (silverchair.com)
  • The particularity of neuroblastoma lies in its development from cells whose embryological maturation into adult sympathetic nerve cells or chromaffin cells is incomplete. (lupinepublishers.com)
  • Decreased expression of NBPF1 in neuroblastoma cell lines with loss of 1p36 heterozygosity and the marked decrease of anchorage-independent clonal growth of DLD1 colorectal carcinoma cells with induced NBPF1 expression further suggest that NBPF1 functions as tumor suppressor. (biomedcentral.com)
  • Using this screening method, the researchers identified a compound that causes neuroblastoma cells to differentiate, uncovering promising new treatment strategies. (sciencedaily.com)
  • Researchers think that giving an extra treatment of high dose chemotherapy with 2 stem cell transplants might be better. (cancerresearchuk.org)
  • The researchers from the Wellcome Sanger Institute, Great Ormond Street Hospital (GOSH) and their collaborators, discovered that all neuroblastomas arise from a single type of embryonic cell called sympathoblasts. (sanger.ac.uk)
  • This varied outlook prompted the researchers to ask whether the range of severity could be caused by neuroblastomas arising from different cell types at different stages of the child's development in the womb. (sanger.ac.uk)
  • We investigated the value of our proprietary approach-cell surface vimentin (CSV) positive circulating tumor cells (CTC) to monitor treatment response and predict relapse in NB patients under remission in a Phase II long-term preventative clinical trial. (psu.edu)
  • Clone NS20Y/MS of the murine neuroblastoma C1300 persistently infected with the wild-type Edmonston strain of MV secretes relatively high levels of alpha/beta interferon (IFN). (bgu.ac.il)
  • The GDNF family ligands (GFLs) function through a glycosyl-phosphatidylinositol-(GPI) anchored coreceptor, GDNF family receptor alpha (GFRα), and rearranged during transfection (RET), a well-known receptor tyrosine kinase involved in kidney development, spermatogonial stem cell maintenance, and the development and maintenance of the sympathetic, parasympathetic, and enteric nervous systems [ 1 , 2 ]. (medsci.org)
  • Mn was demonstrated to be more toxic in the catecholamine-producing CATH.a cells (EC50=60 M) than in non-catecholaminergic SK-N-SH cells (EC50=200 M). To test the hypothesis that the sensitivity of CATH.a cells to Mn is associated with their dopamine (DA) content, DA concentrations were suppressed in these cells by pretreatment with -methyl-para-tyrosine (AMPT). (cdc.gov)
  • We cannot always predict the extent of NKT cell expansion in vivo after infusion," said Dr. Leonid Metelitsa, co-corresponding author and professor of paediatrics - haematology and oncology at Baylor. (ecancer.org)
  • In order to help NKT cells expressing the GD2-CAR (CAR.GD2 NKTs) last longer in patients, we included a mediator known as interleukin-15. (texaschildrens.org)
  • Little is known about changes within the mitochondrial (mt) genome during tumor progression in general and during initiation and progression of neuroblastoma (NB) in particular. (uni-koeln.de)
  • Patient-derived neuroblastoma models may also provide useful information about clonal evolution, phenotypic plasticity, and cell states in relation to neuroblastoma progression. (lu.se)
  • Consistently, T cells from patients are characterized by features of exhaustion, expressing inhibitory receptors and showing low expression of effector cytokines. (curie.fr)
  • We are currently enrolling patients on a phase 1 trial , GINAKIT2, to find the highest and safe dose of GD2-CAR NKT cells (GINAKIT cells) for subsequent study of their anti-tumor activity. (texaschildrens.org)
  • In this case, CARs include a part specific for the GD2 molecule, which is present at high levels in NB tumors and has already led to promising results in clinical trials of GD2-targeted T cells in NB patients. (texaschildrens.org)
  • We hypothesize that CAR.GD2-IL15 NKTs will demonstrate anti-tumor activity in NB patients through a combination of directly killing NB cells and indirectly targeting supportive TAMs. (texaschildrens.org)
  • The differences in outcome for patients with neuroblastoma are striking. (medscape.com)
  • Over the last 2 decades, many chromosomal and molecular abnormalities have been identified in patients with neuroblastoma. (medscape.com)
  • This finding means that it is possible to offer simple, non-invasive screening for patients with a family history of neuroblastoma," said Mossé. (clpmag.com)
  • All patients received multi-agent induction chemotherapy for 6 cycles, including an initial 2 cycles of high-dose cyclophosphamide plus topotecan followed by blood stem cell collection. (oncologynurseadvisor.com)
  • Various karyotypic abnormalities occur, but a deletion of the short arm of chromosome 1 is found in 70-80% of all patients with neuroblastomas. (medscape.com)
  • En revanche, les patients de moins de cinq ans et ceux avec un diagnostic de cancer provisoire posé initialement bénéficiaient du délai total médian le plus court. (who.int)
  • Nous suggérons de mettre en place des programmes de formation médicale continue, d'améliorer l'accès aux services de diagnostic, et de faciliter l'orientation-recours de façon à donner la priorité aux patients suspects de cancer et ainsi raccourcir le délai de diagnostic. (who.int)
  • Neuroblastoma" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (harvard.edu)
  • Close to 50 percent of neuroblastoma cases occur in children younger than two years old. (medicalxpress.com)
  • Because only about 600 new cases of all forms of neuroblastoma occur annually in the US, familial (inherited) neuroblastoma is a very rare subset of a relatively uncommon disease. (clpmag.com)
  • Almost 90% of neuroblastomas occur in children 5 years of age. (msdmanuals.com)
  • Most neuroblastomas occur spontaneously, but 1 to 2% appear to be inherited. (msdmanuals.com)
  • Neuroblastoma is thought to occur sporadically, with 1-2% of cases thought to be familial. (medscape.com)
  • Proteasomal inhibition induced significantly increased alpha-synuclein aggregation in cells expressing mutant alpha-synuclein. (unboundmedicine.com)
  • Lev N, Melamed E, Offen D. Proteasomal inhibition hypersensitizes differentiated neuroblastoma cells to oxidative damage. (unboundmedicine.com)
  • This leads to the competitive inhibition of acyl-CoA(s) binding to hACBP and to the accumulation of lipid droplets inside the IMR-32 cells . (bvsalud.org)