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  • Fibroblasts
  • hESCs can be generated by SCNT using dermal fibroblasts nuclei from both a middle-aged 35-year-old male and an elderly, 75-year-old male, suggesting that age-associated changes are not necessarily an impediment to SCNT-based nuclear reprogramming of human cells. (wikipedia.org)
  • They are collectable from commercially obtainable mesenchymal cells such as human fibroblasts, bone marrow-mesenchymal stem cells and adipose-derived stem cells. (wikipedia.org)
  • Hela cells and human fibroblast-derived iPS cells showed high telomerase activity while Muse was at nearly the same level as that in somatic cells such as fibroblasts (these data are shown without running control for the telomerase activity, the comparison is not scientific thought). (wikipedia.org)
  • Examples include using living fibroblasts in skin replacement or repair, cartilage repaired with living chondrocytes, or other types of cells used in other ways. (wikipedia.org)
  • myoblasts
  • Moreover, autologous transplantation of skeletal myoblasts has been considered because of their high proliferative potential, their commitment to a well-differentiated myogenic lineage, their resistance to ischemia, and their origin, which overcomes ethical, immunological, and availability problems. (ahajournals.org)
  • However, even if phase II clinical trials with autologous skeletal myoblasts are ongoing, several problems related to potentially life-threatening arrhythmia (perhaps reflecting cellular uncoupling with host cardiomyocytes 7 ) must be taken into account when this approach is considered. (ahajournals.org)
  • Regulation of cell proliferation by fast Myosin light chain 1 in myoblasts derived from extraocular muscle, diaphragm and gastrocnemius. (semanticscholar.org)
  • Protein
  • The protein encoded by this gene is a high affinity calcium ion-binding protein that is structurally and functionally similar to calmodulin and troponin C. The encoded protein is thought to be involved in muscle relaxation. (genecards.org)
  • Studies of the mouse and chick homolog suggest the encoded protein may play a role in developing teeth and craniofacial mesenchyme of neural crest origin. (genecards.org)
  • They showed that opposing gradients of bone morphogenetic protein (BMP) and Nodal, two transforming growth factor family members that act as morphogens, are sufficient to induce molecular and cellular mechanisms required to organize, in vivo or in vitro, uncommitted cells of the zebrafish blastula animal pole into a well-developed embryo. (wikipedia.org)
  • In 2014, peripheral supplementation of GDF11 protein (in mice) was shown to ameliorate the age-related dysfunction of skeletal muscle by rescuing the function of aged muscle stem cells. (wikipedia.org)
  • This was in agreement with previous data showing chondroitin sulfate GAG chains attached to bovine perlecan produced by chondrocytes and that recombinant human domain I protein was glycosylated with both heparan and chondroitin sulfate chains when expressed in Chinese Hamster Ovary cells. (wikipedia.org)
  • Ras-related protein M-Ras, also known as muscle RAS oncogene homolog and R-Ras3, is a protein that in humans is encoded by the MRAS gene on chromosome 3. (wikipedia.org)
  • β-catenin is a dual function protein, involved in regulation and coordination of cell-cell adhesion and gene transcription. (wikipedia.org)
  • Beta-catenin was initially discovered in the early 1990s as a component of a mammalian cell adhesion complex: a protein responsible for cytoplasmatic anchoring of cadherins. (wikipedia.org)
  • E3 ubiquitin-protein ligase NEDD4 also known as neural precursor cell expressed developmentally down-regulated protein 4 (NEDD4) is an enzyme that in humans is encoded by the NEDD4 gene. (wikipedia.org)
  • Proteins
  • in association with actin and other contractile proteins it is also a major structural component of the muscle sarcomere. (semanticscholar.org)
  • Neural cells, for example, express growth-associated proteins, such as GAP-43, tubulin, actin, an array of novel neuropeptides, and cytokines that induce a cellular physiological response to regenerate from the damage. (wikipedia.org)
  • ventricular
  • We describe the isolation of undifferentiated cells that grow as self-adherent clusters (that we have termed "cardiospheres") from subcultures of postnatal atrial or ventricular human biopsy specimens and from murine hearts. (ahajournals.org)
  • bone marrow
  • By expressing a dominant negative form of PTPkappa or by using short-hairpin RNA for PTPkappa in bone-marrow derived stem cells, Erdenbayer and colleagues demonstrated that CD4(+) T cells development was inhibited. (wikipedia.org)
  • kidney
  • This vaccine was made possible by the cell culture research of John Franklin Enders, Thomas Huckle Weller, and Frederick Chapman Robbins, who were awarded a Nobel Prize for their discovery of a method of growing the virus in monkey kidney cell cultures. (wikipedia.org)
  • mammalian cells
  • Cells are grown and maintained at an appropriate temperature and gas mixture (typically, 37 °C, 5% CO2 for mammalian cells) in a cell incubator. (wikipedia.org)
  • citation needed] Before this, laboratory cultures of healthy, noncancerous mammalian cells would only divide a fixed number of times, up to the Hayflick limit, before dying. (wikipedia.org)
  • reproductive
  • But I'm trying to understand if there is there anything inherently evil or anti-biblical about cloning (for reproductive purposes). (probe.org)
  • Formation
  • 5) repeated subculturing of these colonies, which lead to the formation of cell lines capable of multiplying indefinitely while preserving the characteristics of ES cells for months and years. (lifeissues.net)
  • Muse cells do not undergo teratoma formation when transplanted into a host environment in vivo. (wikipedia.org)
  • Pattern formation in the morphogenesis of an animal is regulated by genetic induction factors that put cells to work after damage has occurred. (wikipedia.org)
  • cardiac
  • Recent studies have even surmised the existence of resident cardiac stem cells, endothelial cells generating cardiomyocytes by cell contact or extracardiac progenitors for cardiomyocytes, but these findings are still controversial. (ahajournals.org)
  • This report describes the identification and preliminary characterization of cells from the adult human and murine heart, which have the properties of cardiac stem cells. (ahajournals.org)
  • In cardiac muscle, beta-catenin localizes to adherens junctions in intercalated disc structures, which are critical for electrical and mechanical coupling between adjacent cardiomyocytes. (wikipedia.org)
  • transformation
  • The reversible transformation of cells of one differentiated cell type to another is called metaplasia. (wikipedia.org)
  • More currently, Ras family members such as R-RAS, R-RAS2 and also R-RAS3 have also been implicated as main factors in triggering neural transformation, with R-RAS2 as the most significant element. (wikipedia.org)
  • pancreatic
  • PTPkappa interacts by co-immunoprecipitation with E-cadherin, α-catenin and β-catenin in pancreatic acinar cells prior to the dissolution of adherens junctions in a rat model of pancreatitis. (wikipedia.org)
  • The authors suggest that the presence of PTPkappa at the plasma membrane in association with the cadherin/catenin complex is important for the maintenance of adherens junction in pancreatic acinar cells, much as it was suggested above in melanoma cells. (wikipedia.org)
  • Development
  • In 1924 Spemann and Mangold demonstrated the key importance of cell-cell inductions during animal development. (wikipedia.org)
  • During mouse development, GDF11 expression begins in the tail bud and caudal neural plate region. (wikipedia.org)
  • PTPkappa has also been shown to regulate CD4+ positive T cell development. (wikipedia.org)
  • PTPkappa likely regulates T-cell development by positively regulating ERK1/2 phosphorylation via the regulation of MEK1/2 and c-Raf phosphorylation. (wikipedia.org)
  • genetic
  • I would like to know as much information as you have on genetic cloning, so that I can gain an understanding of it and how it works. (probe.org)
  • Cloning produces a nearly identical genetic copy of the original by taking the nucleus of a cell from an organism and placing inside an egg cell of the same species. (probe.org)
  • Currently only bulls are cloned to make more copies of good genetic stock for normal animal husbandry purposes. (probe.org)
  • HeLa
  • Total RNA was isolated from 0.75 million HeLa cells using Norgen's Total RNA Purification Kit, various silica-based competitors and phenol-based TRI Reagent. (biocat.com)
  • Extracellular Matrix
  • Perlecan is a large multidomain (five domains, labeled I-V) proteoglycan that binds to and cross-links many extracellular matrix (ECM) components and cell-surface molecules. (wikipedia.org)
  • Perlecan is synthesized by both vascular endothelial and smooth muscle cells and deposited in the extracellular matrix. (wikipedia.org)
  • Cells can modify their extracellular matrix and basement membranes in response to signals or stress. (wikipedia.org)
  • islet
  • Artificial pancreas: research involves using islet cells to produce and regulate insulin, particularly in cases of diabetes. (wikipedia.org)
  • nucleus
  • The process involves sucking out the nucleus of a somatic (body) cell and injecting it into an oocyte that has had its nucleus removed Using an approach based on the protocol outlined by Tachibana et al. (wikipedia.org)
  • commonly
  • Aside from temperature and gas mixture, the most commonly varied factor in culture systems is the cell growth medium. (wikipedia.org)