Neovascularization endothelial progenitor cells autologous transplantation. Medical search. Frequent questions

However, there are two main principles by which cells facilitate therapeutic action: Stem, progenitor, or mature cell engraftment, differentiation, and long-term replacement of damaged tissue. (wikipedia.org)
These cells then integrate into the site of injury, replacing damaged tissue, and thus facilitate improved function of the organ or tissue. (wikipedia.org)
These early trials are showing roles for stem cells both in replacing damaged tissue as well as in providing extracellular factors that can promote endogenous cellular salvage and replenishment. (biomedcentral.com)
Cells for vascular therapy must be able to home to ischaemic or damaged tissue and engage in vessel formation alone or in unison with resident vasculature to achieve a controlled and functional reperfusion event, without causing pathological angiogenesis (for example, proliferative retinopathy in the vitreous of the eye). (biomedcentral.com)

In vitro experiments withcardiac fibroblasts and H9c2 cells confirmed the exceptional biocompatibilityof BC-Ppy composites. (ibecbarcelona.eu)
Subsequently, the scaffold was bioconjugated with apelin-13 using the EDC-NHS linker and acellular scaffolds were recellularized using fibroblasts, endothelial cells, and smooth muscle cells. (biomedcentral.com)
In cultured primary fibroblasts and cancer cells, the chemotherapeutic drug doxorubicin causes mtDNA damage and release, which leads to cGAS STING dependent ISG activation. (regenerativemedicine.net)
In this study, we reported the fabrication of human triple-layered alveolar lung models comprising of human lung epithelial cells, human endothelial cells, and human lung fibroblasts using the drop-on-demand (DOD) 3D bioprinting technique. (regenhu.com)

The idea of myocardial regeneration with stem cell transplantation after myocardial infarction receives tremendous interest. (escardio.org)
The use of cell therapy for vascular regeneration offers an exciting new prospect in regenerative medicine. (biomedcentral.com)
CD133 + stem cells represent a promising subpopulation for innovative cell-based therapies in cardiovascular regeneration. (biomedcentral.com)
Adult stem cells remain of particular interest as therapeutic agents for cardiac regeneration in ischemic heart disease [ 1 - 4 ]. (biomedcentral.com)
The regeneration potential of these cells is mainly based on their direct contribution to neovascularization and on their secretion of various paracrine factors activating pro-angiogenic mechanisms [ 13 - 16 ]. (biomedcentral.com)
According to the European Medicines Agency (EMA) BM-derived CD133 + stem cells intended for the regeneration of human ischemic heart tissue are classified as an advanced therapy medicinal product (ATMP) [ 17 ]. (biomedcentral.com)
This is Part 3 of a series of contributions on cardiac regeneration after myocardial infarct with stem cells. (pharmaceuticalintelligence.com)
This series of articles discusses the difficulties we have encountered in adopting stem cell research to clinical therapeutics in regeneration of cardiac tissue damaged post myocardial infarct. (pharmaceuticalintelligence.com)
Part I went into some detail on a method of obtaining suitable cells, growing them in sheets, and transferring the sheets to the surface for regeneration and repair, which is now going into clinical trials. (pharmaceuticalintelligence.com)
These cells can be differentiated into mature endothelial cells, which are involved in processes of angiogenesis and vessel regeneration. (intechopen.com)
Thus, it has been shown that early EPCs release cytokines that promote tissue regeneration and neovasculogenesis, whereas late EPC and endothelial colony forming cells (ECFCs) contribute to the formation of blood vessels and stimulate tube formation. (intechopen.com)
This article is intended to provide a review of the most current approaches to utilizing stem cells and bioengineering principles for the purpose of salivary tissue regeneration. (biomedcentral.com)
HBOT facilitates liver regeneration in living donors after liver transplantation and after hepatectomy. (revitalife.co.in)
Later, Arnold Caplan's group described mesenchymal stem/stromal cells (MSCs) as multipotent mesenchymal cell populations which can differentiate into several tissue types, and demonstrated roles for MSCs in the regeneration of bone, cartilage or ligaments in animal and clinical studies [2-4]. (stemcellslab.net)
Endothelial cells (ECs) may elaborate essential "angiocrine factors" involved in organ regeneration. (biomedcentral.com)
He is developing a treatment strategy for abdominal aortic aneurysms by delivering mesenchymal stem cells or their secreted products to the periadventitial side of the aneurysm to inhibit the matrix degradation commonly seen in the disease progression and promote its regeneration. (pitt.edu)

14. Sun X, Fang B, Zhao X, Zhang G, Ma H. Preconditioning of mesenchymal stem cells by sevoflurane to improve their therapeutic potential. (sciendo.com)
While numerous teams continue to refine and expand the role of bone marrow and cord blood stem cells for their vanguard uses in blood and immune disorders, many others are looking to expand the uses of the various types of stem cells found in bone marrow and cord blood, in particular mesenchymal stem cells, to uses beyond those that could be corrected by replacing cells in their own lineage. (biomedcentral.com)
There are many studies involving autologous therapies and some allogenic therapies, based on the recovery of mobilized bone marrow cells, including mesenchymal stem cells (MSCs) and adipose derived stem cells that also include the stromal or adherent cell type that has an MSC phenotype. (biomedcentral.com)
With other REMEDI researchers we are specifically studying the immune therapeutic properties of mesenchymal stem cells (MSC). (universityofgalway.ie)
Ma DD , 2007 , 'Expression of Neurofilament Proteins in Adult Bone Marrow Mesenchymal Stem Cells and Embryonic Stem Cells and Their Clinical Potential' , in Davenport LP (ed. (edu.au)
Singer NG, Caplan AI Mesenchymal stem cells: mechanisms of inflammation. (seffiline.com)
Delarosa O, Dalemans W, Lombardo E Mesenchymal stem cells as therapeutic agents of inflammatory and autoimmune diseases. (seffiline.com)
Krampera M, Glennie S, Dyson J et al Bone marrow mesenchymal stem cells inhibit the response of naive and memory antigenspecific T cells to their cognate peptide. (seffiline.com)
Caplan, A. I. Adult mesenchymal stem cells for tissue engineering versus regenerative medicine. (seffiline.com)
Here he also utilizes mesenchymal stem cells or their products incorporated in a biodegradable scaffold that undergoes substantial in vivo remodeling to develop a native-like blood vessel. (pitt.edu)

Transplantation of autologous corneal epithelial stem/progenitor cells (CESCs) expanded in culture has successfully restored vision and revolutionized the treatment of limbal stem cell deficiency (LSCD), which is a major cause, either primary or secondary, of significant visual loss and blindness in many corneal disorders including aniridia. (uclahealth.org)
Because the maintenance of stem cell characteristics in the process of cell culture expansion is essential for the success of ocular surface reconstruction, the small molecules generated in this project may also be helpful in the development of novel pharmaceutical reagents for treating other corneal epithelial disorders. (uclahealth.org)
Within minutes after a small corneal epithelial injury, cells at the edge of the abrasion begin to migrate centripetally to cover the defect rapidly at a rate of 60-80 µm/h. (medscape.com)

Cell therapy (also called cellular therapy, cell transplantation, or cytotherapy) is a therapy in which viable cells are injected, grafted or implanted into a patient in order to effectuate a medicinal effect, for example, by transplanting T-cells capable of fighting cancer cells via cell-mediated immunity in the course of immunotherapy, or grafting stem cells to regenerate diseased tissues. (wikipedia.org)
In obese humans, adipocyte-secreted CCL20 may contribute to the deposition of Compact disc4+ helper and Compact disc8+ cytotoxic T lymphocytes within adipose tissues, possibly via connections with CCR6 which was upregulated on T cells in obese adipose tissues (100). (exposed-skin-care.net)
However, the main element substances that mediate T cell infiltration into adipose tissues in maturing remain to become discovered. (exposed-skin-care.net)
Activation of Typical T Cells in Adipose Tissues Compact disc4+ Purpureaside C T Cell Activation TCRs recognize the current presence of a particular antigen by binding to brief peptide sequences in the antigen that's shown on APCs. (exposed-skin-care.net)
further defined that mostly huge adipocytes from obese adipose tissues exhibited an increased expression degree of MHCII substances and acted as APCs to activate Compact disc4+ T cells to secrete IFN- (103). (exposed-skin-care.net)
In vascular medicine, various stem cells and adult progenitors have been highlighted as having a vasoreparative role in ischaemic tissues. (biomedcentral.com)
The evaluation of the most suitable timing of cell delivery as well as the number of cells needed to integrate into resident vasculature and promote revascularisation of specific tissues requires careful optimisation and evaluation. (biomedcentral.com)
hEPCs have been used for cell-based therapies due to their capacity to contribute in the re-endothelialization of injured blood vessels and neovascularization in ischemic tissues. (intechopen.com)
In clinical practice, these involve the life-long immunosuppression of the organ transplantation patients, the creation of the supporting environment for engineered tissues in the mended organ, the unsolved issues of cell survival and differentiation of the cell-based therapy, and the selection and development of vectors for gene therapy. (biomedcentral.com)
In order to address the limits associated with current therapies, many research groups have investigated various regenerative medicine strategies utilizing different stem cell sources to engineer artificial salivary tissues that can mitigate the effects of xerostomia and hyposalivation. (biomedcentral.com)
Cells Tissues Organs. (wakehealth.edu)
Here is a general list of all the Ways Stem Cells heal tissues or create new tissue. (drcremers.com)
These elaborate signals promoted by ECs have been termed "angiocrine factors" and have been shown to be crucial for the maintenance of organ-specific tissues and tumor cells [ 12 ]. (biomedcentral.com)
Therefore, we propose that damage to and subsequent release of mtDNA elicits a protective signalling response that enhances nDNA repair in cells and tissues, suggesting that mtDNA is a genotoxic stress sentinel. (regenerativemedicine.net)

Moreover, it seems that paracrine action of skeletal myoblasts facilitate neighboring cardiomyocytes to maintain their replicative potential and/or stimulate differentiation of native cardiac stem cells (8,9). (escardio.org)
Furthermore, we demonstrated that these automatically isolated cells bear proliferation and differentiation capacities comparable to manually isolated cells in vitro. (biomedcentral.com)
Recently, several clinical trials showed an increased left ventricular ejection fraction (LVEF) and an improved regional perfusion following intramyocardial application of cluster of differentiation (CD) 133 + stem cells [ 5 - 9 ]. (biomedcentral.com)
Until recently, differentiation was thought to be the primary function of regenerative cells. (vetstem.com)
1 One strategy is to seed the appropriate cells on a biodegradable scaffold engineered with the desired mechanical properties, followed by stimulation of cell growth and differentiation in vitro, such that, on implantation in vivo, the engineered construct undergoes remodeling and maturation into functional tissue. (ashpublications.org)
Specifically, the employment of BC-Ppy compositesdrives partial H9c2 differentiation toward a cardiomyocyte-like phenotype.The scaffolds increase the expression of functional cardiac markersin H9c2 cells, indicative of a higher differentiation efficiency,which is not observed with plain BC. (ibecbarcelona.eu)
It Increases Endogenous and peripheral Stem cell mobilisation, activation homing and differentiation potential hence useful adjunct in regenerative medicine. (revitalife.co.in)
microRNAs have emerged as master regulators of stem cell lineage differentiation and angiogenesis [ 14 ]. (biomedcentral.com)
Chun SY, Mack DL, Moorefield E, Oh SH, Kwon TG, Pettenati MJ, Yoo JJ, Coppi PD, Atala A, Soker S. Pdx1 and controlled culture conditions induced differentiation of human amniotic fluid-derived stem cells to insulin-producing clusters. (wakehealth.edu)

Why Use Adipose-Derived Stem Cells? (vetstem.com)
Hyperbaric oxygen therapy also increases viability and proliferation of adipose-derived stem cells, alters marker expression and subpopulations, decreases TGF-β secretion. (revitalife.co.in)
To reduce possible xenobiotic contamination from 3T3s, primary human adipose-derived stem cells (ASCs) were examined as feeder cells to support the expansion of LSCs in vitro . (drcremers.com)
Mei H, González S, Nakatsu MN, Baclagon ER, Chen FV, Deng SX (2017) Human adipose-derived stem cells support the growth of limbal stem/progenitor cells. (drcremers.com)
Human adipose-derived stem cells impair natural killer cell function and exhibit low susceptibility to natural killer-mediated lysis. (seffiline.com)

We investigated whether catheter-based, intramyocardial transplantation of autologous endothelial progenitor cells can enhance neovascularization in myocardial ischemia. (stemyoon.org)
These favorable outcomes encourage future clinical trials of catheter-based, intramyocardial transplantation of autologous CD34+ MNCs in the setting of chronic myocardial ischemia. (stemyoon.org)
Several clinical trials have shown remarkable beneficial effects following their intramyocardial transplantation. (biomedcentral.com)

In the swine study, ischemic area by NOGA mapping, Rentrop grade angiographic collateral development, and echocardiographic left ventricular ejection fraction improved significantly 4 weeks after transplantation of NA/CD31+ MNCs but not after injection of NA/CD31- MNCs or PBS. (stemyoon.org)
Capillary density in ischemic myocardium 4 weeks after transplantation was significantly greater in the NA/CD31+ MNC group than the control groups. (stemyoon.org)
In addition, the regenerative potential of purified stem cells was assessed 3 weeks after transplantation in immunodeficient mice which had been subjected to experimental myocardial infarction. (biomedcentral.com)
The heart was explanted at 2, 6 and 1 2 weeks after transplantation for histological studies. (jgc301.com)
The mature blood vessel index was highest in group-4 at 6 and 1 2 weeks after transplantation. (jgc301.com)

The method of stimulating isolated cells with CSE in vitro has been explored and frequently applied to determine the direct causes in the relationships between cigarette smoking and cellular functions [ 15 ]. (hindawi.com)
In this study, in attempt to provide fresh information about the impact of CS on proliferation of EPCs to elucidate the pathophysiological mechanisms of the diseases related to CS in cellular level, we assessed the proliferation of EPCs after interfering the cells with a series of concentrations of CSE for various times of exposure in vitro . (hindawi.com)
Her research interests include the preparation and design of materials and scaffolds for in vitro and in vivo fundamental studies, and a further focus is the provision of useful tools to assess mechanisms that govern cell behavior in regenerative medicine. (ibecbarcelona.eu)
Un rôle majeur des prostaglandines dans l'hématopoïèse, ainsi mis en évidence, a pu être étendu à la souris chez qui un traitement par la prostaglandine PGE2 provoque la multiplication des CSH in vivo et in vitro, un résultat qui génerera très probablement des applications cliniques. (hypotheses.org)
The most efficient method to expand limbal stem cells (LSCs) in vitro for clinical transplantation is to culture single LSCs directly on growth-arrested mouse fibroblast 3T3 cells. (drcremers.com)
The aim of this study is to investigate the role of activated ovarian endothelial cells in early in-vitro follicular development. (biomedcentral.com)
This model is a novel platform for the in vitro maturation of early follicles and for the future exploration of endothelial-follicular communication. (biomedcentral.com)
The structure and function of the patches are studied in vitro, and cardiac cell morphology is assessed after transplantation, revealing elongated cardiomyocytes with massive actinin striation. (regenhu.com)

This includes bone and cartilage repair, cell types into which MSCs readily differentiate, and immune conditions such as graft versus host disease and autoimmune conditions that utilize the MSC's immune suppressive properties. (biomedcentral.com)
The use of patient's own bone marrow aspirates, hematopoietic stem cells and MSCs, for heart muscle tissue repair can be puzzling because these cells do not normally contribute to the cardiac lineage types that are desired. (biomedcentral.com)
Mesenchymal stromal cells (MSCs) have shown benefit in other inflammatory diseases. (unav.edu)
Objectives: To evaluate the safety and feasibility of endobronchial administration of bone marrow autologous MSCs (BM-MSC) in patients with mild-to-moderate IPF. (unav.edu)
Methods: A phase I multicentre clinical trial (ClinicalTrials.gov NCT01919827) with a single endobronchial administration of autologous adult BM-MSCs in patients diagnosed with mild-to-moderate IPF. (unav.edu)
Mesenchymal stem/stromal cells (MSCs) are increasingly used as an intravenously applied cellular therapeutic. (stemcellslab.net)
Pivotal studies by the group of Horwitz in children with osteogenesis imperfecta, an inherited enzyme deficiency of collagen synthesis by mesenchymal cells in bone, opened the field for intravenous use of MSCs. (stemcellslab.net)
Therefore, the authors concluded that transplantation of isolated healthy allogeneic MSCs might cure the disease. (stemcellslab.net)
In a second study [6], these authors showed that autologous, enzyme-deficient MSCs transduced with a copy of the intact gene resulted in normal collagen production in bone cavities. (stemcellslab.net)
The tissue source of the MSCs was in most cases not decisive, and cells from various tissue sources were explored. (stemcellslab.net)
and signals from the injected cells were found early after administration of the MSCs at the highest frequencies in the lungs, followed by liver and spleen. (stemcellslab.net)

Using an organotypic angiogenesis co-culture assay, we showed that GSK429286 formed a dense vascular network with thicker endothelial tubes. (bvsalud.org)
Herein, we developed a novel, high content 2D angiogenesis assay that captures endothelial morphogenesis's cellular processes, including lumen formation. (bvsalud.org)
Both in animal models and humans, it has been shown that cell therapy can induce therapeutic angiogenesis, making mesenchymal stromal cell-based therapy one of the most promising therapeutic alternatives. (unav.edu)
This chapter provides an overview of the key role of hEPC in promoting angiogenesis and their potential use for cell therapy. (intechopen.com)
Other benefits include fibroblast proliferation via fibroblast growth factor (FGF), capillary angiogenesis, decreasing oedema, the upregulation of vascular endothelial growth factor (VEGF), decreasing nitric oxide (NO) leading to vasoconstriction, cell proliferation and migration, wound granulation, and improved epithelial production. (revitalife.co.in)
Cell therapy, however, holds the promise of repleting the damage heart with new contractile cells that can be engi-neered to secrete concoctions that promote healing by recruiting new blood vessel development or angiogenesis. (jgc301.com)

Hematopoietic Stem Cell Transplantation in Refractory Crohn's Disease: Should It Be Considered? (mdpi.com)

More research teams are accelerating the use of other types of adult stem cells, in particular neural stem cells for diseases where beneficial outcome could result from either in-lineage cell replacement or extracellular factors. (biomedcentral.com)
Part I will be confined to the importance of source of progenitor cells, whether adult stem cells or umbilical cord blood. (pharmaceuticalintelligence.com)
Human endothelial progenitor cells (hEPCs) are adult stem cells, located in the bone marrow and peripheral blood. (intechopen.com)
uses a concentrated form of autologous adipose-derived adult stem cells to treat traumatic and degenerative diseases, including bowed tendons, ligament injuries, osteoarthritis, and osteochondral defects in horses, dogs, and cats. (vetstem.com)
Adult stem cells turn out to be a more robust and reliable source of cell to heal damaged cells. (drcremers.com)
These adult stem cells, such as from fat (adipose derived) or bone marrow, have the ability to transform into different cells. (drcremers.com)

The stromal fraction that is harvested from adipose tissue is a heterogeneous mixture of regenerative cells (see below). (vetstem.com)
VetStem Regenerative Cell Therapy is based on a clinical technology licensed from Artecel Inc. Original patents are from the University of Pittsburgh and Duke University. (vetstem.com)
Why use adipose-derived regenerative cells rather than regenerative cells derived from bone marrow? (vetstem.com)
VetStem Regenerative Cell (VSRC™) therapy delivers a functionally diverse cell population able to communicate with other cells in their local environment. (vetstem.com)
However, the functions of regenerative cells are now known to be much more diverse and are implicated in a highly integrated and complex network. (vetstem.com)
4. Stem cell and regenerative medicine related immunology. (universityofgalway.ie)
Mesenchymal stem cell-based therapy: A new paradigm in regenerative medicine. (seffiline.com)

In 2021 Atara biotherapeutics became the first ever allogeneic T cell therapy company to be reviewed by any regulatory agency in the world (EMA) Cell therapy is targeted at many clinical indications in multiple organs and by several modes of cell delivery. (wikipedia.org)
Preemptive immunotherapy in childhood acute myeloid leukemia for patients showing evidence of mixed chimerism after allogeneic stem cell transplantation. (shengsci.com)
Previous studies have shown that children with acute myeloid leukemia (AML) who developed mixed chimerism (MC) were at high risk for relapse after allogeneic stem-cell transplantation (allo-SCT). (shengsci.com)

Epithelial cells adjacent to the area of the defect flatten, lose their hemidesmosome attachments, and migrate on transient focal contact zones that are formed between cytoplasmic actin filaments and extracellular matrix proteins. (medscape.com)
To optimize the ASC-supported culture, freshly isolated limbal epithelial cells in the form of single cells (SC-ASC) or cell clusters (CC-ASC) were cultured using three different methods: LSCs seeded directly on feeder cells, a 3-dimensional (3D) culture system and a 3D culture system with fibrin (fibrin 3D). (drcremers.com)
CC-ASC generated epithelial cells with undifferentiated morphology in all culture methods, among which CC-ASC in 3D culture supported the highest cell doubling (cells doubled 9.0 times compared to cells doubled 4.9 times in control) while maintained the percentage of putative limbal stem/progenitor cells compared to the control. (drcremers.com)

Cell therapy is a potential new treatment for post MI patients, but the main challenges are : the necessity to preserve immune competency and to gain adequate nutrition and homing signals necessary for stem cells' engraftment and survival. (escardio.org)
Various cell types have been tested experimentally for cardiac repair so far, but only those of autologous origin have yet undergone clinical testing due to immune competency. (escardio.org)
In more recent work, cell encapsulation is pursued as a means to shield therapeutic cells from the host immune response. (wikipedia.org)
A significant proportion of clinical studies that are underway involve bone marrow and cord blood stem cells for blood and immune disorders [ 3 ] and cancers. (biomedcentral.com)
2 Examples of this approach include blood vessels and cardiovascular substitutes, where autologous vascular cells have been used for this purpose without immune rejection. (ashpublications.org)
However, though BC is emerging as a potential organ transplant option, challenges regarding organ size scalability, immune system incompatibilities, long-term maintenance, potential evolutionary distance, or unveiled mechanisms between donor and host cells remain. (frontiersin.org)
I developed specific interest in the immune-modulating properties of Vitamin D, the molecular basis for chronic kidney transplant rejection, complications of organ transplantation and the involvement of cells from the immune system in kidney disease. (universityofgalway.ie)
Transduction of CLL cells by CD40 ligand enhances an antigen-specific immune recognition by autologous T cells. (shengsci.com)
An induced state of non-reactivity to grafted tissue from a donor organism that would ordinarily trigger a cell-mediated or humoral immune response. (lookformedical.com)

Several preliminary reports have demonstrated that local stem cell transplantation in patients with acute myocardial infarction is safe and may lead to improved myocardial function and perfusion. (escardio.org)
Progenitor cell therapy in a porcine acute myocardial infarction model induces cardiac hypertrophy, mediated by paracrine secretion of cardiotrophic factors including TGFbeta1. (sciendo.com)

Therapy promotes neovascularization and endothelial cells, fibroblast proliferation and collagen deposition. (revitalife.co.in)
Through integrated analysis of single cell chromatin landscapes and gene expression states, coupled with spatial transcriptomic profiling, we are able to impute fibroblast epigenomes with temporospatial resolution. (stanford.edu)

As such, stem cell therapy cannot be regarded as a valid therapeutic option for patients with cardiovascular disease in the present era of evidenced-based medicine, yet it holds great hope for the future. (escardio.org)
Preclinical and some small-scaled clinical trials have suggested feasibility and safety of cardiac stem cell therapy. (escardio.org)
Repair of scar tissue constitutes a challenge for cardiac stem cell therapy due to lack of adequate nutrition and homing signals necessary for stem cells' engraftment and survival. (escardio.org)
3-6 The first peer-reviewed double-blinded multicenter study for adipose-derived stem cell therapy use in canine osteoarthritis of the hip showed significant improvement in all post treatment evaluation times for lameness, pain, and range of motion. (vetstem.com)
Blood-derived endothelial progenitor cells (EPCs) represent the "promoters" of vascular repair providing the rationale for autologous stem cell therapy [ 2 ]. (biomedcentral.com)

Number and migratory activity of circulating endothelial progenitor cells inversely correlate with risk factors for coronary artery disease. (sciendo.com)
In addition, the number and the migratory activity of these cells are inversely correlated with risk factors such as hypertension, hypercholesterolemia, diabetes, and metabolic syndrome. (intechopen.com)

The bone morrow contains several stem cell types including hematopoetic stem cells, endothelial progenitor cells, mesenchymal (stromal) stem cells and multipotent adult progenitor cells. (escardio.org)
In this paradigm multipotent or unipotent cells differentiate into a specific cell type in the lab or after reaching the site of injury (via local or systemic administration). (wikipedia.org)

The aim of this study is to evaluate the feasibility, safety, and efficacy of cell therapy based on mesenchymal stromal cells derived from adipose tissue intramuscular administration to patients with type 2 diabetes mellitus with critical limb ischemia and without possibility of revascularization. (unav.edu)
This concept started from the observation that bone marrow transplantation can provide stromal cells able to synthesize intact collagen type I, replacing deficient patient cell function and ameliorating disease symptoms [5]. (stemcellslab.net)
Stromal cells that surround follicles in the secondary stage or later become organized into thecal layers, in which the innermost part contains blood vessels. (biomedcentral.com)

Cell therapy is currently emerging as a potential new treatment for post MI patients with the assumption that recolonization of the areas of scarred myocardium with exogenously supplied surrogates or precursors of cardiomyocytes can restore function and ultimately affect clinical outcomes. (escardio.org)
Intramyocardial, Autologous CD34+ Cell Therapy for Refractory Angina / Novelty and Significance. (sciendo.com)
Cell therapy originated in the nineteenth century when scientists experimented by injecting animal material in an attempt to prevent and treat illness. (wikipedia.org)
Cell therapy can be defined as therapy in which cellular material is injected or otherwise transplanted into a patient. (wikipedia.org)
The origins of cell therapy can perhaps be traced to the nineteenth century, when Charles-Édouard Brown-Séquard (1817-1894) injected animal testicle extracts in an attempt to stop the effects of aging. (wikipedia.org)
In 1931 Paul Niehans (1882-1971) - who has been called the inventor of cell therapy - attempted to cure a patient by injecting material from calf embryos. (wikipedia.org)
For a long time, bone marrow transplantation was the only clinically applicable method of cell transplantation, however, since the 1990s, cell therapy has been investigated for a wide scale of pathologies and disorders. (wikipedia.org)
Cell therapy provided a novel approach to effectuate therapeutic efficacy. (wikipedia.org)
Cell therapy offers a new strategy that supports the introduction of new and active cells to restore previously compromised or deteriorated tissue- and organ structures. (wikipedia.org)
As a consequence cell therapy as a strategy has been attracting significant investments by commercial entities which suggest strong prospects for future growth. (wikipedia.org)
A cell therapy approach should be aimed at promoting revascularisation of ischaemic tissue. (biomedcentral.com)
Finally, one should consider cell choice, a critical aspect of any cell therapy. (biomedcentral.com)
Also, the multidisciplinary coordination exercise needed to develop this clinical trial protocol will undoubtfully be useful to conduct academic clinical trials in the field of cell therapy in the near future. (unav.edu)
Depending on the patient's medical condition, a refractory disease patient also requires an on-time selective option, such as less invasive cellular therapy options or curative organ transplantation that can function immediately after transplantation. (frontiersin.org)
Thus, umbilical cord blood EPCs hold great therapeutic potential for cell therapy and vascular engineering. (biomedcentral.com)
My laboratory group has received funding from SFI to study the immunologic basis of acute and chronic kidney disease and how this might be altered by stem cell-based therapy. (universityofgalway.ie)
Transplantation of ex vivo expanded LSCs to the LSCD eye has been reported as a successful therapy to treat LSCD [ 5 , 11 , 12 ]. (drcremers.com)
Autologous stem cell transplantation for treatment of rectovaginal fistula in perianal Crohn's disease: a new cell-based therapy. (seffiline.com)
Eighty-two patients with advanced multiple myeloma (MM) were enrolled in 2 sequential clinical studies of 1 or 2 courses of myeloablative therapy with stem cell support. (shengsci.com)
Human Myoblast Genome Therapy (HMGT) is a platform technology of cell transplantation, nuclear transfer, and tissue engineering. (jgc301.com)

Transplantation of ex vivo expanded endothelial progenitor cells for therapeutic neovascularization. (sciendo.com)
We hypothesize that blood-derived endothelial progenitor cells (EPCs) have the required proliferative and vasculogenic activity to create vascular networks in vivo. (ashpublications.org)
In addition, mtDNA stress in TFAM-deficient mouse melanoma cells produces tumours that are more resistant to doxorubicin in vivo. (regenerativemedicine.net)

Autologous transplantation of 10(7) NA/CD31+ MNCs, 10(7) NA/CD31- MNCs, or PBS was performed with a NOGA mapping injection catheter to target ischemic myocardium. (stemyoon.org)
Endothelial progenitor cells in neovascularization of infarcted myocardium. (sciendo.com)
Enormous problems have been encountered in the selection of progenitor cells, the growth into compatible and functional myocardial tissue, and the survival of the myocardium. (pharmaceuticalintelligence.com)
Treatment with G-CSF further increased the number of vessels in the myocardium, possibly due to mobilization of bone marrow cells. (pharmaceuticalintelligence.com)

Recently, there has been growing interest in the therapeutic potential of using a cell-based approach to treat vasodegenerative disorders. (biomedcentral.com)
In particular, we focus on the therapeutic potential of endothelial progenitor cells as an exciting new option for the treatment of ischaemic diseases. (biomedcentral.com)

indicated that ATMs colocalized with T cells in lymphoid clusters within adipose tissue and may act as APCs, which express high levels of MHCII and also costimulatory molecules and process and present antigens to induce CD4+ T-cell proliferation and activation in adipose tissue of obese mice (29, 68, 105). (exposed-skin-care.net)
showed that adipose tissue from obese mice induced proliferation of splenic CD8+ T cells, indicating a CD8+ T cell-activating environment in obese adipose tissue (31). (exposed-skin-care.net)
A sufficient supply of progenitor stem cells to facilitate epithelial cell proliferation is important for the cornea. (medscape.com)
can improve renal hypoxia by increasing partial pressure of dissolved (non-haemoglobin-bound) oxygen without affecting it's demand hence significantly benefitting patients with Chronic Renal Failure.it also recruits tissue and peripheral progenitors and supplies the optimal environment crucial for their proliferation and for tissue repair. (revitalife.co.in)
Furthermore, the results demonstrated considerable elevation of cell adhesion and proliferation in scaffolds bioconjugated with apelin-13. (biomedcentral.com)
The 3D bioprinted human triple-layered alveolar lung models were able to maintain cell viability with relative similar proliferation profile over time as compared to non-printed cells. (regenhu.com)

In experimental and clinical studies unselected mononuclear bone marrow cells as well as specific subpopulations have been used for transplantation. (escardio.org)
In recent years, clinical trials with stem cells have taken the emerging field in many new directions. (biomedcentral.com)
The rapid advance of stem cell clinical trials for a broad spectrum of conditions warrants an update of the review by Trounson (2009) [ 1 ]. (biomedcentral.com)
There has been a rapid surge in clinical trials involving stem cell therapies over the last two to three years and those trials are establishing the clinical pathways for an emergent new medicine. (biomedcentral.com)
Placenta-derived stem cells are being considered for similar uses and are in Phase III clinical trial for critical limb ischemia by Israel's Pluristem Therapeutics. (biomedcentral.com)
We have chosen to concentrate on the emerging therapeutics that broadly involves a wide range of cell types in clinical trials registered on the National Institutes of Health's clinical trials web site. (biomedcentral.com)
This review will examine the clinical potential of several stem and progenitor cells that may be utilised to regenerate defunct or damaged vasculature and restore blood flow to the ischaemic tissue. (biomedcentral.com)
Yet, CD133 + stem cells for clinical use are purified using manual or semi-automatic devices based on the conventional magnetic cell sorting (MACS®) technique. (biomedcentral.com)
Despite successes in pre-clinical animal models with stem cells, a problem arises with respect to the biology of the transplanted progenitor cells. (pharmaceuticalintelligence.com)
Success in numerous animal models of disease and emerging success in human clinical trials for Crohn's fistulas 1 and stroke, 2 along with hundreds of ongoing clinical trials (see sidebar) support the rationale for stem cell use, and now success, in veterinary medicine. (vetstem.com)
He also spearheaded an international effort to identify the first Mendelian germline predisposition gene in testicular germ cell tumors, CHEK2, with potentially immediate clinical and mechanistic implications. (the-asci.org)
My interest in transplantation resulted in a research fellowship in basic immunology at the University of Chicago, USA following which I spent 9 years combining clinical transplant medicine with laboratory-based immunology research at Mayo Clinic. (universityofgalway.ie)
9. Clinical complications and outcomes of human kidney and pancreas transplantation. (universityofgalway.ie)
The standard method to culture LSCs on 3T3 feeder cells that have been used in clinical study is cultivating single LSC directly on top of the growth-arrested 3T3 feeder cells [ 14 ]. (drcremers.com)
Engraftment, clinical, and molecular follow-up of patients with multiple myeloma who were reinfused with highly purified CD34+ cells to support single or tandem high-dose chemotherapy. (shengsci.com)

Optimizing stem cell function for the treatment of ischemic heart disease. (sciendo.com)

Proliferative neovascularization of the retina is associated with many common retinal diseases, including ROP. (uclahealth.org)

Following, the two cell types are separately combined with hydrogels to form bioinks for the parenchymal cardiac tissue and blood vessels. (regenhu.com)

In recent decades, however, stem cell and cell transplantation has gained significant interest by researchers as a potential new therapeutic strategy for a wide range of diseases, in particular for degenerative and immunogenic pathologies. (wikipedia.org)
However, in many diseases and disorders, cell are compromised by e.g. senescence, limited blood supply (ischemia), inflammation, or simply a reduction in the number of cells. (wikipedia.org)
Endothelial progenitor cells (EPCs) contribute to neovascularization and play an important role in the development of these diseases. (hindawi.com)
Organ transplantation is the ultimate treatment option for patients suffering from refractory diseases. (frontiersin.org)

Another statement showed the preadipocyte- and endothelial cell-derived stromal-derived element-1 (CXCL12), mediated early infiltration of CD4+ T lymphocytes in obesity, which preceded the increase of macrophages in adipose cells of mice on HFD (101). (exposed-skin-care.net)
Indeed, the principal adipocytes isolated from obese mice could induce antigen-specific Compact disc4+ T cell activation (58). (exposed-skin-care.net)
To test this, EPCs isolated from human umbilical cord blood or from adult peripheral blood, and human saphenous vein smooth muscle cells (HSVSMCs) as a source of perivascular cells, were combined in Matrigel and implanted subcutaneously into immunodeficient mice. (ashpublications.org)
After a constitutive activation of the Akt pathway was initiated, early follicles (50-150 um) were mechanically isolated from 8-day-old mice and co-cultured with these activated ovarian endothelial cells (AOEC) ( n = 32), gel ( n = 24) or within matrigel ( n = 27) in serum free media for 14 days. (biomedcentral.com)

Increased Stem cell release from the bone marrow results in increased CD34 cells many folds. (revitalife.co.in)

The ideal treatment option for terminally ill patients is organ transplantation. (frontiersin.org)
To take human organ generation via BC and transplantation to the next step, we reviewed current emerging organ generation technologies and the associated efficiency of chimera formation in human cells from the standpoint of developmental biology. (frontiersin.org)
The general underlying strategy behind the development of tissue engineered organ substitutes is the utilization of a combination of cells, biomaterials, and biochemical cues intended to recreate the natural organ environment. (biomedcentral.com)
It is used for the prophylaxis of graft rejection in organ and tissue transplantation. (lookformedical.com)

The number of colony-forming cells (CFCs) also increases substantially. (revitalife.co.in)

Unlike stem cells, myoblasts are differentiated, immature cells destined to become muscles. (jgc301.com)

Four weeks after constrictor placement, CD31+ mononuclear cells (MNCs) were freshly isolated from the peripheral blood of each animal. (stemyoon.org)
These include bone marrow-derived cells, peripheral blood derived stem cells and skeletal myoblasts (3). (escardio.org)
Ruengsakulrach P, Visudharom K, Chaothawee L, Belkin M. Safety and Efficacy of Intramyocardial Implantation of Peripheral Blood Stem Cell for Cardiomyopathy. (tci-thaijo.org)
EPCs from human umbilical cord and adult peripheral blood activate different mechanisms upon high-dose x-ray radiation treatment: CB-EPCs undergo p53 stabilization, Bax-dependent apoptosis and p21-dependent G 1 and G 2 /M cell cycle checkpoints, while PB-EPCs undergo only radiation-induced senescence [ 13 ], indicating unique gene expression patterns in EPCs of different sources. (biomedcentral.com)
At least 6.5 x 10(8) [corrected] mononuclear cells/kg patient weight were collected from the peripheral blood of each patient, cyropreserved, and returned intravenously following CBV administration. (shengsci.com)
Recent studies have shown that soluble factors elaborated by human T lymphocytes enhance erythroid burst formation by human peripheral blood null cells. (shengsci.com)

Bone marrow transplants are the most common and well established cell transplantation therapies. (wikipedia.org)

Stem cell-like human endothelial progenitors show enhanced colony-forming capacity after brief sevoflurane exposure: preconditioning of angiogenic cells by volatile anesthetics. (sciendo.com)
Although such attempts produced no positive benefit, further research found in the mid twentieth century that human cells could be used to help prevent the human body rejecting transplanted organs, leading in time to successful bone marrow transplantation as has become common practice in treatment for patients that have compromised bone marrow after disease, infection, radiation or chemotherapy. (wikipedia.org)
in 1968, in Minnesota, the first successful human bone marrow transplantation took place. (wikipedia.org)
We showed that a small molecule Wnt inhibitor developed in my laboratory that targets the Dvl PDZ domain blocks the oxidative stress-induced neovascularization in human retinal vascular endothelial cells. (uclahealth.org)
In addition, we use novel Wnt small molecule regulators to investigate whether these modulators would affect Dex-mediated phenotype on primary human TM cells. (uclahealth.org)
Quantitative analyses showed the microvessel density was significantly superior to that generated by human dermal microvascular endothelial cells (HDMECs) but similar to that generated by human umbilical vein endothelial cells (HUVECs). (ashpublications.org)
Plasticity of human adipose lineage cells toward endothelial cells: physiological and therapeutic perspectives. (seffiline.com)
Enhancement of human erythroid progenitor cell growth by media conditioned by a human t-lymphocyte line. (shengsci.com)
The polyvinylpyrrolidone-based bio-inks and the use of a 300 mm nozzle diameter improved the repeatability of the bioprinting process by achieving consistent cell output over time using different human alveolar lung cells. (regenhu.com)
Here, we describe a 3D multi-cellular human omentum tissue model, which considers the spatial arrangement of five omental cell types. (regenhu.com)

Our results proposed 16-h perfusion of 0.25% sodium dodecyl sulfate (SDS) + 0.5% Triton X-100 combination to the vessel as an optimal decellularization protocol in terms of cell elimination as well as extracellular matrix preservation. (biomedcentral.com)
While the cells are reprogrammed to become pluripotent stem cells, and differentiated to cardiomyocytes and endothelial cells, the extracellular matrix is processed into a personalized hydrogel. (regenhu.com)

Interestingly, contradictory data exist for transformation of bone marrow derived progenitor cells (BMPC) into new cardiomyocytes and alternative mechanisms as enhanced neovascularization, enhanced scar tissue formation due to augmented inflammatory response and decreased apoptosis have been suggested for the benefical effects of these cells on myocardial function after myocardial infarction (4). (escardio.org)
A quantitative, randomized study evaluating three methods of mesenchymal stem cell delivery following myocardial infarction. (sciendo.com)
The progressive impairment of endothelial function and integrity starts a cascade of events, leading to microcirculation damage, atherosclerosis and common cardiovascular disease (CVD), such as coronary heart disease (CHD), myocardial infarction (MI), heart failure, stroke and peripheral arterial disease (PAD) [ 1 ]. (biomedcentral.com)

To determine the safety and efficacy of intramyocardial autologous blood stem cell injection for cardiomyopathy. (tci-thaijo.org)
Between May 2005 and February 2010, 126 consecutive patients underwent intramyocardial cell injection. (tci-thaijo.org)
The number of cells prior to injection was 46.1 ± 36.5 million cells. (tci-thaijo.org)
Combined coronary artery surgery and cell injection were performed in 33.8% of ICM cases. (tci-thaijo.org)
In the 1970s, Friedenstein and colleagues [1] first reported that locally applied culture-expanded populations of bone marrow stroma-derived fibroblastic cells remained at their injection sites under the kidney capsule, where an ectopic hematopoiesis was initiated. (stemcellslab.net)

The bone-marrow-derived endothelial progenitor cells (EPCs) provide an alternative source of endothelial cells (ECs) that contributes to neovessel formation in endothelium structure [ 4 , 5 ]. (hindawi.com)
Different phenotypes and subtypes of endothelial progenitor cells (EPCs), such as early and late EPCs, have been described according to their functionality. (intechopen.com)
Endothelial progenitor cells (EPCs) play a fundamental role in post-natal vascular repair, yet EPCs from different anatomic locations possess unique biological properties. (biomedcentral.com)
EPCs from CB expressed abundant genes involved in cell cycle, hypoxia signalling and blood vessel development, correlating with the phenotypes that CB-EPCs proliferated more rapidly, migrated faster, and formed tubule structure more efficiently. (biomedcentral.com)
Knocking down miR-31 levels in CB-EPCs suppressed cell migration and microtubule formation, while overexpressing miR-31 in PB-EPCs helped to recapitulate some of CB-EPC functions. (biomedcentral.com)

however, the drawback of this approach is that this may result in cells localising to non-target organs such as the liver, kidneys, spleen and lung. (biomedcentral.com)

Although there is no definitive evidence, combinations of progenitor cells seem to be more benefical than specific stem cell type for cardiac repair (3). (escardio.org)

The pathogenic mechanisms involve a wide range of cells and soluble factors. (mdpi.com)
Reliable and predictive experimental models are urgently needed to study metastatic mechanisms of ovarian cancer cells in the omentum. (regenhu.com)

Numerous cytokines and growth factors that are up-regulated in corneal cells further contribute to tissue inflammation. (medscape.com)
microRNAs also play a crucial role in endothelial inflammation, senescence and susceptibility to atherosclerosis: endothelial inflammation is critically regulated by miRNAs such as miR-126 and miR-10a, and endothelial aging is additionally controlled by miR-217 and miR-34a [ 15 ]. (biomedcentral.com)

Mobilized bone marrow cells repair the infarcted heart, improving function and survival. (sciendo.com)

6 To overcome the problem of vascularization, strategies such as embedding angiogenic factors into the scaffold to promote ingrowth of microvessels, fabrication technologies to create polymers containing vessellike networks, and prevascularization of matrices prior to cell seeding have been proposed. (ashpublications.org)
We also developed a novel strategy to eliminate the remnant detergents from the scaffold and increase cell viability by incubating acellular scaffolds with Bio-Beads SM-2 resin. (biomedcentral.com)
These findings reveal that the incubation of the scaffold with Bio-Beads SM-2 is a novel and promising approach for increasing cell viability and growth within the scaffold. (biomedcentral.com)
In conclusion, our results provide a platform in which xenograft vessels are decellularized properly in a short time, and the recellularization process is significantly improved after the bioconjugation of the acellular scaffold with apelin-13 in terms of cell adhesion and viability within the scaffold. (biomedcentral.com)

Moreover, the harvest of vessels prolongs the surgical procedure and it may lead to the morbidity of donor site in elder patients: therefore, it seems that the use of tissue-engineered vessels would be an attractive and less invasive substitute for autologous vascular grafts. (biomedcentral.com)

Basal and limbal stem cells contribute to mitosis. (medscape.com)
A deficiency of limbal stem cells, from either disease (eg, aniridia) or trauma (eg, chemical burn), can preclude adequate epithelial wound healing. (medscape.com)
Loss of LSCs or their dysfunction may lead to limbal stem cell deficiency (LSCD) which present with corneal opacity, vascularization and conjunctivalization. (drcremers.com)

Myoblasts cultured from satellite cells of adult muscle biopsies survive, develop, and function to revitalize degenerative muscles upon transplantation. (jgc301.com)

Anatomy38

Organisms12

Diseases30

Chemicals and Drugs63

Analytical, Diagnostic and Therapeutic Techniques and Equipment74

Phenomena and Processes37

Disciplines and Occupations2

Technology, Industry, Agriculture2

Information Science1

Named Groups2

Health Care10

Damaged tissue4

Fibroblasts4

Regeneration16

Mesenchymal stem10

Corneal epithelial3

Tissues14

Differentiation9

Adipose derived stem cells5

Intramyocardial transplantation3

Weeks after transplantation5

Vitro8

MSCs11

Angiogenesis6

Hematopoietic Stem Cell Trans1

Adult stem6

Regenerative7

Allogeneic3

Epithelial cells3

Immune9

Acute myocardial2

Fibroblast2

Stem cell th5

Migratory activity2

Multipotent2

Stromal cells3

Therapy20

Vivo3

Myocardium4

Therapeutic potential2

Proliferation6

Clinical15

Ischemic1

Proliferative1

Blood vessels1

Diseases4

Mice4

CD341

Organ4

Colony formi1

Immature1

Peripheral blood6

Therapies1

Human10

Extracellular2

Myocardial3

Injection5

EPCs5

Liver1

Cardiac1

Mechanisms2

Inflammation2

Bone marro1

Scaffold4

Less invasive1

Limbal stem3

Degenerative1