Neonatal rat ventricular myocytes adult rat cardiomyocytes. Medical search. Frequent questions

The pathological consequences associated with normal cardiovascular aging include hypertrophy, altered left ventricular (LV) diastolic and systolic function, heart failure, enhanced arterial stiffness, and endothelial dysfunction, all of which can alter the structure and function of the heart and arterial system [ 5 , 6 ] . (oaepublish.com)
A large number of studies have found that in heart failure, atrial fibrillation, myocardial hypertrophy and myocardial infarction, atrial and ventricular HCN2 and HCN4 channels gene express unusually causing atrial or ventricular myocyte If current rise or fall, and all of these may be associated with heart disease fatal arrhythmia [18] . (bjcvs.org)
Basing on the presence of If current in ventricular myocytes isolated from severely hypertrophied rat hearts, the current arrhythmogenic role in cardiac hypertrophy and failure has been inferred [25] , and its density is larger in human ventricular myocytes isolated from the hearts of patients with ischemic than in those with dilated cardiomyopathy [21] . (bjcvs.org)
Systemic hypertension increases cardiac workload causing cardiomyocyte hypertrophy and increased cardiac fibrosis. (ox.ac.uk)
However, it suppressed hypertension-induced cardiac hypertrophy resulting from angiotensin II (0.8 mg/[kg·d], 7d), with inhibition of Nppa/Nppb mRNA upregulation, reduced cardiomyocyte hypertrophy and, notably, significant reductions in interstitial and perivascular fibrosis. (ox.ac.uk)

Alveoli, pulmonary vessels, α -smooth muscle actin content in pulmonary arterioles, size of cardiomyocytes, right to left ventricular wall diameter ratio, and endothelin-1 plasma concentrations were assessed. (biomedcentral.com)
Macitentan treatment significantly increased pulmonary vessel count (p = 0.004) and decreased right to left ventricular wall diameter ratios (p = 0.002). (biomedcentral.com)

In conclusion, our findings indicate that tyrosine phosphorylation is involved in Fas signaling in ventricular myocytes, and can, therefore, serve as a novel potential target for attenuating Fas-mediated dysfunction in normoxic and hypoxic myocardium. (haifa.ac.il)
Adult rats were subjected to myocardial ischemia/reperfusion (I/R) injury with or without ischemic preconditioning (IPC), and the level of miR‑133b‑5p in myocardium was measured. (spandidos-publications.com)
During hypertension and heart failure, If activity is observed in the ventricular myocardium due to re-expression of HCN genes [18,21-23] , several studies proved that If current density and occurrence is significantly greater in hypertrophic cardiomyocytes and end-stage failing hearts and this is directly related to the arrhythmias [18,21,24-27] . (bjcvs.org)
1 , 2 , 3 , 4 The myocardium is made up of many cell types however the greatest mass of tissue is accounted for by cardiomyocytes. (substack.com)
Numerous studies have documented that cardiomyocyte apoptosis occurs in border zone of infarct scars and in the remote zone of non-infarcted myocardium [ 2 - 4 ], which exacerbates the post-MI remodeling and aggravates cardiac dysfunction [ 5 ]. (ijbs.com)

Nowadays, more and more studies have revealed that cells in the cardiovascular system (such as cardiomyocytes, vascular smooth muscle cells, endothelial cells, et al. (frontiersin.org)
One complementary therapy could be 're-activating' vasculogenesis (i.e. the differentiation of precursor cells into mature endothelial cells), a mechanism that occurs in the heart during development but is quiescent in adult hearts. (elifesciences.org)

ZP123 increases gap junctional conductance and prevents reentrant ventricular tachycardia during myocardial ischemia in open chest dogs. (ku.dk)

Our data speculated that STA protects H/R injury and inhibits oxidative stress and apoptosis in cardiomyocytes by activation of the SIRT1-Nrf2 pathway. (biomedcentral.com)

In addition, neonatal rat cardiomyocytes (NRCMs), cardiac fibroblasts (CFs), and macrophages were isolated for in vitro experiments. (hindawi.com)
Herein, we introduce a cardiac-mimetic cell-culture system that resembles the microenvironment in the heart and provides interactions with cardiomyocytes and electrical cues to the cultured fibroblasts for direct cardiac reprogramming. (thno.org)
The human neonatal dermal fibroblasts containing cardiac transcription factors were plated on the membrane and cultured with the murine cardiomyocyte in the presence of the electric stimulus. (thno.org)
Nano-thin and nano-porous membranes in the culture system facilitated interactions between fibroblasts and cardiomyocytes in coculture. (thno.org)
The cardiac-mimetic culture system may serve as an effective tool for producing a feasible number of reprogrammed cardiomyocytes from fibroblasts. (thno.org)
Direct cardiac reprogramming of fibroblasts is an attractive therapeutic strategy for treating cardiovascular diseases because the adult heart has a low regeneration capacity [ 1 ]. (thno.org)

Upregulation of miR‑133b‑5p contributes to HPC‑mediated cardioprotection in cardiomyocytes, and the mechanism may be associated with inhibition of caspase‑8 and caspase‑3 apoptotic signaling. (spandidos-publications.com)
[18] demonstrated for the first time comparing the mRNA and protein expression of HCN subunits in the human atrial and ventricle under normal and heart failure conditions in human heart failure, an upregulation of ventricular HCN2 and HCN4 underlies the increase in functional If current, Michael et al. (bjcvs.org)

Since protein tyrosine phosphorylation has been shown to be involved in Fas signaling, we have hypothesized that inhibiting tyrosine kinases will attenuate Fas-mediated effects in ventricular myocytes in normoxic and hypoxic conditions. (haifa.ac.il)
miR-195 level was dynamically regulated in response to MI and significantly increased in ischemic regions 24 h post-MI as well as in hypoxic or H 2 O 2 -treated cardiomyocytes. (ijbs.com)
Apoptosis in both hypoxic and H 2 O 2 -treated cardiomyocytes were markedly reduced and cell viability was increased by miR-195 inhibitor. (ijbs.com)
Hypoxic cardiac myocytes generate excess H+ through increased anaerobic glycolysis, net hydrolysis of ATP, and CO2 retention. (biomedres.us)

Qian Y, Qian F, Zhang W, Zhao L, Shen M, Ding C, Guo J ( 2019 ) Shengjiang Powder ameliorates myocardial injury in septic rats by downregulating the phosphorylation of P38-MAPK. (multichannelsystems.com)
A cell-based functional assay system measuring hypertrophic cell growth of neonatal rat ventricle cardiomyocytes (NRVMs) following phenylephrine treatment was applied, and changes in phosphorylation of individual differentially phosphorylated sites were induced by incorporation of phosphorylation competition peptides conjugated with cell-penetrating peptides. (molcells.org)
Gene therapy displacement of endogenous MCUb with a dominant-negative MCUb transgene (MCUb W246R/V251E ) in vivo rescued T2D cardiomyocytes from metabolic inflexibility and stimulated cardiac contractile function and adrenergic responsiveness by enhancing phospholamban phosphorylation via protein kinase A. We conclude that MCUb represents one newly discovered molecular effector at the interface of metabolism and cardiac function, and its repression improves the outcome of the chronically stressed diabetic heart. (diabetesjournals.org)

In addition, recent studies showed that components extracted from Leonurus heterophyllus sweet alleviated left ventricular dysfunction or remodeling in animal models. (biomedcentral.com)
the most prominent is cardiotoxicity which can manifest acutely as well as years after treatment has been discontinued leading to left ventricular dysfunction, dilated cardiomyopathy and heart failure [ 2 - 4 ]. (oncotarget.com)
The damaged muscle is prone to lethal cardiac arrythmias as well as having the potential to develop both right and left ventricular dysfunction (cardiomyopathy). (substack.com)

Myocyte-restricted Hmox1 transgenic mice exposed to MI exhibited significantly improved survival and LV function, lower interstitial fibrosis and oxidative stress. (springer.com)

Therefore, we tested the effect of the tyrosine kinases inhibitors, genistein (50 μmol/l) and herbimycin A (50 μg/ml), on the abovementioned Fas-mediated effects in cultured neonatal rat ventricular myocytes (NRVM) and in freshly isolated adult murine ventricular myocytes. (haifa.ac.il)
Fas receptor was activated by incubating NRVM with recombinant Fas ligand (rFasL, 50 ng/ml) and enhancing antibody (1 μg/ml), or by incubation of murine ventricular myocytes with the Fas-activating antibody Jo2 (10 μg/ml). (haifa.ac.il)
Accordingly, genistein or herbimycin A abolished the diastolic [Ca 2+ ] i -rise (as measured by fura-2 fluorescence) and arrhythmogenic activity in both rat and murine ventricular myocytes, and the Fas-mediated changes in I to and I Ca,L in murine ventricular myocytes. (haifa.ac.il)

Male Wistar rats were subjected to coronary artery ligation, and primary neonatal rat ventricular myocytes were treated with hypoxia or hydrogen peroxide (H 2 O 2 ). (ijbs.com)

Here we investigated roles for miR-126 in stress kinase activation, induction of inflammatory cytokines and apoptosis caused by exposure of cultured cardiac myocytes to hypoxia/acidosis or acidosis alone. (biomedres.us)

Toxic effects of Dox include cardiomyocyte damage and apoptotic and necrotic cell death [ 5 ]. (oncotarget.com)
BDNF mitigated the pro-apoptotic effect of miR-195 in rat cardiomyocytes. (ijbs.com)

Amend N, Thiermann H, Worek F, Wille T ( 2019 ) The arrhythmogenic potential of nerve agents and a cardiac safety profile of antidotes - A proof-of-concept study using human induced pluripotent stem cells derived cardiomyocytes (hiPSC-CM). Toxicol Lett 308:1-6. (multichannelsystems.com)

To investigate this hypothesis, the H9c2 cardiomyocyte cell line was used to establish an in vitro myocardial H/R injury model to explore the roles and potential mechanisms of STA in myocardial H/R injury. (biomedcentral.com)
Here, we investigate the cytotoxic effects of in vitro QD labeling on MSC proliferation and differentiation and use as a cell label in a cardiomyocyte co-culture. (biomedcentral.com)
In addition, functional integration of QD labeled MSCs in an in vitro cardiomyocyte co-culture was assessed. (biomedcentral.com)
We assessed the ability to track QD labeled MSCs in an in vitro cardiomyocyte co-culture. (biomedcentral.com)

Treatment with the gap junction modifier rotigaptide (ZP123) reduces infarct size in rats with chronic myocardial infarction. (ku.dk)
Short-term cardiac stress, induced by ischemia-reperfusion (I/R) injury resulted in impaired left ventricular (LV) recovery and increased infarct size in heterozygous Hmox1-deficient (Hmox1 +/− ) mice [ 55 ]. (springer.com)

Both anatomical abnormalities, including atrial dilatation and abnormal ventricular structures, and functional deficits such as reduced blood flow and reduced strength of contractions were observed. (e-enm.org)
Finally, in a cardiomyocyte co-culture QD labeled MSCs were easy to locate and formed functional cell-to-cell couplings, assessed by dye diffusion. (biomedcentral.com)

Neonatal rat cardiomyocytes were isolated and subjected to hypoxia/reoxygenation (H/R) injury, with or without HPC. (spandidos-publications.com)
Hypoxia/reoxygenation (H/R)-induced cardiomyocyte cell apoptosis is critical in developing myocardial infarction. (biomedcentral.com)
Rat cardiomyocyte H9c2 cells underwent H/R (hypoxia for 4 h and reoxygenation for 12 h). (biomedcentral.com)

There is a growing body of evidence which demonstrates that following ACS, microRNAs might inhibit fibroblast proliferation and scarring, as well as harmful apoptosis of cardiomyocytes, and stimulate fibroblast reprogramming into induced cardiac progenitor cells. (archivesofmedicalscience.com)
In this review, we focus on the role of cardiomyocyte-derived and cardiac fibroblast-derived microRNAs that are involved in the regulation of genes associated with cardiomyocyte and fibroblast function and in atherosclerosis-related cardiac ischemia. (archivesofmedicalscience.com)

Dox-induced over-production of reactive oxygen and nitrogen species (ROS and RNS) such as superoxide, hydrogen peroxide, hydroxyl radical and peroxynitrite has been proposed to be the main cause of Dox-induced acute cardiomyocyte toxicity and cell death by damaging various molecular constituents and organelles [ 9 - 12 ]. (oncotarget.com)
Studies have also suggested that both acute substantial cardiomyocyte loss and chronic low levels of apoptosis contributed to the development of heart failure [ 6 , 7 ]. (ijbs.com)

Our previous studies suggested that brain-derived neurotrophic factor (BDNF)/tropomyosin-related kinase B (TrkB) axis inhibited cardiomyocyte apoptosis in myocardial infarction (MI). (ijbs.com)

Propranolol may increase oxygen requirements by increasing left ventricular fiber length, end diastolic pressure, and systolic ejection period. (ncats.io)

Archives of Disease in Childhood Fetal and Neonatal Edition. (westminster.ac.uk)

In the healthy adult heart, HCN channels are predominantly expressed in the conduction system, especially in the sinoatrial node, HCN4 has been determined as the principal HCN isoform in sinoatrial node cells. (bjcvs.org)
4 , 5 , 6 Cardiomyocytes are the principal contractile cells and are supported by specialized conduction and stromal cell types. (substack.com)
QRS complex duration (index of ventricular conduction) and the first derivative of left ventricular pressure (index of contractility) were measured. (silverchair.com)
In conclusion, using a whole organ preparation, the authors demonstrated that bupivacaine induces similar impairment in ventricular conduction in newborn and adult rabbits. (silverchair.com)
Also, contractility was less impaired than ventricular conduction in both groups. (silverchair.com)

Combined effect of neonatal sympathectomy and adrenal demedullation on blood pressure and vascular changes in spontaneously hypertensive rats. (mcmaster.ca)
Therefore, an intervention at the ET-1 receptor level might have a positive effect on pulmonary vascular resistance and right ventricular remodelling. (biomedcentral.com)

Kraushaar U, Guenther E ( 2019 ) Assay Procedures for Compound Testing of hiPSC-Derived Cardiomyocytes Using Multiwell Microelectrode Arrays. (multichannelsystems.com)
Tahrir FG, Gordon J, Feldman AM, Cheung J, Khalili K, Mohseni Ahooyi T ( 2019 ) Evidence for the impact of BAG3 on electrophysiological activity of primary culture of neonatal cardiomyocytes. (multichannelsystems.com)

Ma S, Ma J, Tu Q, Zheng C, Chen Q, Lv W ( 2020 ) Isoproterenol Increases Left Atrial Fibrosis and Susceptibility to Atrial Fibrillation by Inducing Atrial Ischemic Infarction in Rats. (multichannelsystems.com)
Hang P, Sun C, Guo J, Zhao J, Du Z. BDNF-mediates Down-regulation of MicroRNA-195 Inhibits Ischemic Cardiac Apoptosis in Rats. (ijbs.com)
The present study was performed to investigate the role of miR-195 and the interplay between BDNF and miR-195 in ischemic cardiomyocyte apoptosis. (ijbs.com)
Up-regulation of miR-195 in ischemic cardiomyocytes promotes ischemic apoptosis by targeting Bcl-2. (ijbs.com)
Cardiac myocyte cell death by apoptosis accompanies heart disease of both ischemic and nonischemic origin [1-3]. (biomedres.us)
Inhibition of Na+/H+ exchange is protective against ischemic injury, while inhibition of the vacuolar ATPase promotes apoptosis, in part by shifting the proton load toward the Na+/H+ transporter and thus increasing Ca2+ uptake, and in part by reducing the myocyte capacity to control [pH]i. (biomedres.us)

However, the role of STA on the H/R injury of cardiomyocytes through the SIRT1-Nrf2 pathway remains unexplored. (biomedcentral.com)
recently demonstrated that the developmental VEGFA-MEF2 pathway, which was thought to be involved, is in fact impaired in adult ischaemic hearts. (elifesciences.org)

Chronic hypoxia in the presence of high glucose leads to progressive acidosis of cardiac myocytes in culture. (biologists.com)
To illustrate the utility of the new method, we also examined the chronic effects of aldosterone on [Na + ] i in cultured cardiomyocytes. (biomedcentral.com)
The endothelin-1 receptor antagonist macitentan attenuated cardiovascular remodelling in an infant rat model for preterm chronic lung disease. (biomedcentral.com)
Our group reported that severe chronic hypoxia alone does not cause apoptosis of cardiac myocytes in culture. (biomedres.us)

Using a recombinant endonuclease-deficient Cas9-based gene promoter pulldown approach coupled with mass spectrometry, we found that MCUb is upregulated in the T2D heart due to loss of glucose homeostasis regulator nuclear receptor corepressor 2 repression, and chromatin immunoprecipitation assays identified peroxisome proliferator-activated receptor α as a mediator of MCUb gene expression in T2D cardiomyocytes. (diabetesjournals.org)

Cardiovascular diseases often cause substantial heart damage and even heart failure due to the limited regenerative capacity of adult cardiomyocytes. (thno.org)

Upon myocardial infarction (MI) immune system becomes activated by extensive necrosis of cardiomyocytes and release of damage-associated molecular patterns [ 16 ]. (springer.com)

HPC protected neonatal rat cardiomyocytes against H/R injury by increasing cell viability, while reducing LDH release and cell apoptosis. (spandidos-publications.com)

The major findings were that genistein prevented Fas-mediated increase in 1,4,5-IP 3 production in NRVM (quantified by ion-exchange chromatography): 216 ± 41 counts per minute (cpm) in control, 605 ± 184 cpm in FasL-treated cardiomyocytes and 137 ± 51 cpm in rFasL + genistein-treated cultures. (haifa.ac.il)

However, the concentration leading to 50% decrease in the first derivative of left ventricular pressure was much higher than the concentration leading to half maximum increase in QRS complex duration. (silverchair.com)
MiR-126 expression in serum is positively correlated with left ventricular ejection fraction (LVEF). (justia.com)

Cells were pretreated with STA (50 µM) 2 h before H/R. Cardiomyocyte injury was evaluated by CCK-8 assay and lactate dehydrogenase (LDH) release. (biomedcentral.com)
BDNF level in rat ventricles was measured by enzyme linked immunosorbent assay (ELISA). (ijbs.com)

However, the knockdown of miR‑133b‑5p in the cardiomyocytes blocked HPC‑mediated cardioprotection as reflected by the aggravation of cell injury and apoptosis. (spandidos-publications.com)
In the heart, cardiomyocytes interact with neighboring cells through direct cell-cell interactions, various secreted cytokines, and electric signals (Figure 1 A) [ 24 ]. (thno.org)
Mechanisms by which Dox promotes cardiac damage and cardiomyocyte cell death have been investigated extensively, but a definitive picture has yet to emerge. (oncotarget.com)
Cardiomyocytes require large numbers of healthy-functioning mitochondria to ensure sufficient ATP production for contractile function and cell survival. (oncotarget.com)
The results support significant roles for miR-126 in regulating cardiac myocyte survival pathways and cell death during exposure to simulated ischemia and acidosis. (biomedres.us)

Therefore, the study aimed to investigate the protective effects and mechanisms of STA on H/R injury of cardiomyocytes. (biomedcentral.com)
The results demonstrated that this 96-well plate based measurement has merits, especially for screening test of compounds regulating [Na + ] i , and is useful to elucidate the mechanisms and consequences of altered [Na + ] i handling in cardiomyocytes. (biomedcentral.com)
My laboratory's overall goal is to (i) understand the mechanisms of right heart failure in children and adults with congenital heart disease and (ii) to develop biomarkers as a plasma signature of myocardial events to better understand the mechanisms of heart failure, improve monitoring of disease progression, early detection of heart failure and risk-stratification. (stanford.edu)

Studies were included if they had prospectively collected data on all-cause mortality in adult septic patients with either plasma BNP or NT-proBNP measurement. (biomedcentral.com)

Moreover, inhibition of miR-195 significantly improved cardiac function of MI rats. (ijbs.com)

ASK1 was activated during ischemia in adult rat hearts, but not on reperfusion, consistent with activation by moderate (not high) reactive oxygen species levels. (ox.ac.uk)

KCNQ channels are involved in the regulatory volume decrease response in primary neonatal rat cardiomyocytes. (ku.dk)

Meanwhile, BDNF protein level was rapidly increased in MI rats and H 2 O 2 -treated cardiomyocytes. (ijbs.com)

Because the free drug concentration is considered to be the toxic moiety, it is highly plausible that neonates and infants may be more prone to the cardiotoxic effects of bupivacaine than older children and adults. (silverchair.com)

Thus, successfully reversal of cardiomyocyte apoptosis during early stage of MI is crucial for repairing the injured heart and ameliorating cardiac function [ 8 ]. (ijbs.com)
The authors compared the effects of bupivacaine on an isolated heart preparation in newborn and adult rabbits. (silverchair.com)

Biomarkers, Socioeconomic Factors, and Right Ventricular Function After Surgical Repair for Tetralogy of Fallot. (stanford.edu)

Diabetes results in a cardiomyopathy characterized by decreased cardiomyocyte function, increased myocardial fatty acid utilization, decreased glucose utilization, increased myocardial oxygen consumption, and decreased cardiac efficiency ( 4 , 5 ), primarily as the result of changes in metabolism and Ca 2+ handling within the myocyte ( 3 , 6 , 7 ). (diabetesjournals.org)

Cardiac function and apoptosis were detected in MI rats intravenously injected with antagomiR-195. (ijbs.com)

Using immunoblotting, we established that moderate levels of H2O2 activate ASK1 in neonatal rat cardiomyocytes and perfused rat hearts. (ox.ac.uk)
ASK1 was not activated by IL1β in cardiomyocytes and activation in perfused hearts was due to increased reactive oxygen species. (ox.ac.uk)
Selonsertib (ASK1 inhibitor) prevented activation of p38-MAPKs (but not JNKs) by oxidative stresses in cultured cardiomyocytes and perfused hearts. (ox.ac.uk)
Adult and newborn rabbit hearts were exposed to step-increasing concentrations of bupivacaine. (silverchair.com)
No difference was observed between neonatal and adult hearts. (silverchair.com)

The aim of this study was to investigate whether resveratrol (RSV) could ameliorate ischemia- and hypoxia-associated cardiomyocyte apoptosis and injury via inhibiting senescence signaling and inflammasome activation. (hindawi.com)

Anatomy26

Organisms12

Diseases13

Chemicals and Drugs141

Analytical, Diagnostic and Therapeutic Techniques and Equipment17

Phenomena and Processes43

Disciplines and Occupations2

Information Science1

Health Care1

Hypertrophy5

Right to left ventricu2

Myocardium5

Endothelial cells2

Myocardial ischemia1

Apoptosis in cardiomyocytes1

Fibroblasts6

Upregulation2

Hypoxic4

Phosphorylation3

Dysfunction3

Transgenic mice1

Murine3

Coronary1

Hypoxia-acidosis1

Apoptotic2

Arrhythmogenic1

Vitro4

Infarct2

Functional2

Reoxygenation3

Fibroblast2

Acute2

Kinase1

Diastolic1

Fetal1

Conduction5

Vascular2

20192

Ischemic6

Pathway2

Chronic4

Receptor1

Cardiovascular1

Molecular1

Viability1

Findings1

Left2

Assay2

Cell5

Mechanisms3

Septic1

Inhibition1

Reperfusion1

Decrease1

Rapidly1

Children and adults1

Heart2

Factors1

Glucose1

Function1

Hearts5

Investigate1