Here, we investigate the cytotoxic effects of in vitro QD labeling on MSC proliferation and differentiation and use as a cell label in a cardiomyocyte co-culture. (biomedcentral.com)
In addition, functional integration of QD labeled MSCs in an in vitro cardiomyocyte co-culture was assessed. (biomedcentral.com)
We assessed the ability to track QD labeled MSCs in an in vitro cardiomyocyte co-culture. (biomedcentral.com)
2018). Development of bovine embryos in vitro in coculture with murine mesenchymal stem clls and embryonic fibroblasts. (sciendo.com)
Fibroblasts6
Herein, we introduce a cardiac-mimetic cell-culture system that resembles the microenvironment in the heart and provides interactions with cardiomyocytes and electrical cues to the cultured fibroblasts for direct cardiac reprogramming. (thno.org)
The human neonatal dermal fibroblasts containing cardiac transcription factors were plated on the membrane and cultured with the murine cardiomyocyte in the presence of the electric stimulus. (thno.org)
Nano-thin and nano-porous membranes in the culture system facilitated interactions between fibroblasts and cardiomyocytes in coculture. (thno.org)
The cardiac-mimetic culture system may serve as an effective tool for producing a feasible number of reprogrammed cardiomyocytes from fibroblasts. (thno.org)
Direct cardiac reprogramming of fibroblasts is an attractive therapeutic strategy for treating cardiovascular diseases because the adult heart has a low regeneration capacity [ 1 ]. (thno.org)
We after that cocultured outrageous type cardiomyocytes with either cardiomyocytes or fibroblasts overexpressing R1R2 and noticed greater than a twofold upsurge in the level and price of contraction of outrageous type cardiomyocytes. (healthcarecoremeasures.com)
Endothelial cells1
Nowadays, more and more studies have revealed that cells in the cardiovascular system (such as cardiomyocytes, vascular smooth muscle cells, endothelial cells, et al. (frontiersin.org)
Mesenchymal1
Comparison of human mesenchymal stromal cells from four neonatal tissues: Amniotic membrane, chorionic membrane, placental decidua and umbilical cord. (sciendo.com)
Differentiation1
Specifically, the employment of BC-Ppy compositesdrives partial H9c2 differentiation toward a cardiomyocyte-like phenotype.The scaffolds increase the expression of functional cardiac markersin H9c2 cells, indicative of a higher differentiation efficiency,which is not observed with plain BC. (ibecbarcelona.eu)
Embryonic1
Embryonic or adult SCs by definition are characterized by their self-renewal and potentiality, peculiarities that allow them to give rise to more SCs and differentiate into various cell lineages under appropriate conditions. (biomedcentral.com)
Transplantation1
The transplantation of individual pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) is a promising technique to treat myocardial infarction and reverse heart failure but to time the contractile benefit generally in most studies remains humble. (healthcarecoremeasures.com)
Stem cells1
In this study, we describe the formation of three-dimensional (3D) cell aggregates ("cardiospheres") by putative stem cells isolated from adult dog myocardium using poly-L-ornithine. (bvsalud.org)
Cells4
Biochemicalanalyses revealed that H9c2 cells showed different cardiomyocyte phenotypesand distinct levels of maturity depending on the amount of Ppy inthe substrate used. (ibecbarcelona.eu)
Older cardiospheres developed foci of "beating" cells upon co-culture with rat neonatal cardiomyocytes. (bvsalud.org)
In the heart, cardiomyocytes interact with neighboring cells through direct cell-cell interactions, various secreted cytokines, and electric signals (Figure 1 A) [ 24 ]. (thno.org)
The immunomodulatory effects of enzymatic and bacterial produced HMOS, 2'FL and 3FL, were studied in a coculture model combining the crosstalk between human intestinal epithelial cells and immune cells, which is critical in mucosal immune development. (mdpi.com)
Human1
Human milk oligosaccharides (HMOS) possess immunomodulatory properties essential for neonatal immune development. (mdpi.com)
Properties1
The incidence of linked to expression of an orange pigment with antioxidant invasive GBS disease in nonpregnant adults is growing, in properties ( 6 , 15 ), and these 2 factors act in concert to im- particular in elderly persons and in those with chronic un- pair macrophage-based immune clearance ( 16 ). (cdc.gov)
Cell2
Within these cocultures heterogeneous doublets with one GFP+ cell and GFP-null cell frequently produced (Fig. 5A). (healthcarecoremeasures.com)
Finally, in a cardiomyocyte co-culture QD labeled MSCs were easy to locate and formed functional cell-to-cell couplings, assessed by dye diffusion. (biomedcentral.com)
Endothelial3
Nowadays, more and more studies have revealed that cells in the cardiovascular system (such as cardiomyocytes, vascular smooth muscle cells, endothelial cells, et al. (frontiersin.org)
MSCs are also capable of differentiation into cardiomyocytes, endothelial cells, and vascular smooth muscle cells, although the relative contribution of trilineage differentiation and paracrine effectors on cardiac repair remains the subject of active investigation. (springer.com)
Cardiomyocyte-specific inhibition of sox9b function resulted in a decrease in ventricular cardiomyocytes, an increase in atrial cardiomyocytes, hypoplastic endothelial cushions, and impaired epicardial development, ultimately culminating in heart failure. (nature.com)
Proliferation2
Adult hearts are characterized by inefficient regeneration after injury, thus, the features that support or prevent cardiomyocyte (CM) proliferation are important to clarify. (nyu.edu)
Macrophages trigger cardiomyocyte proliferation by increasing epicardial vegfaa expression during larval zebrafish heartregeneration, 2022, Developmental Cell, Jun 9. (medicalresearchscotland.org.uk)
Progenitor1
Stem and progenitor cell-derived cardiomyocytes (SPC-CMs) hold great promise for myocardial repairs. (ca.gov)
Cardiac5
[ 76 ] Upon stimulation with 5-azacytidine in culture, Sca-1 cells express cardiac genes and structural proteins and can differentiated into cardiomyocytes. (medscape.com)
It has been reported that regulation of the expression of H3K9me2 during cardiomyocyte maturation can affect the expression of cardiac development-related genes ( 9 ). (spandidos-publications.com)
Montserrat N, Jopling C, Izpisua Belmonte JC (2010) Understanding the molecular basis for cardiomyocyte cell cycle regulation: new insights in cardiac regeneration after injury? (springer.com)
Beltrami AP, Barlucchi L, Torella D et al (2003) Adult cardiac stem cells are multipotent and support myocardial regeneration. (springer.com)
Cardiomyocyte-specific dnsox9b expression significantly reduced end diastolic volume, which corresponded with a decrease in stroke volume, ejection fraction, and cardiac output. (nature.com)
Cardiospheres2
In this study, we describe the formation of three-dimensional (3D) cell aggregates ("cardiospheres") by putative stem cells isolated from adult dog myocardium using poly-L-ornithine. (bvsalud.org)
Older cardiospheres developed foci of "beating" cells upon co-culture with rat neonatal cardiomyocytes. (bvsalud.org)
Maturation1
We will apply information obtained from hESC-CM experiments to induce EP maturation of cardiomyocytes derived from various iPSCs. (ca.gov)
Ventricular2
Ebf1-deficient hearts display myocardial hypercellularity and reduced cardiomyocyte size, ventricular conduction system hypoplasia, and conduction system disease. (nyu.edu)
Here, using the conduction system expression marker Cntn2-GFP and the conduction system lineage marker Etv1Cre ERT2 , we demonstrate that Purkinje CMs that comprise the adult ventricular conduction system are disproportionately diploid (33%, vs. 4% of bulk ventricular CMs). (nyu.edu)
Fetal2
Sca-1 cells have been identified with the use of mouse Sca-1 antibody, which binds to a homogenous population of cells in fetal and adult human hearts. (medscape.com)
In contrast, a significant fraction of conduction CMs persist as diploid cells from fetal life and avoid neonatal cell cycle activity. (nyu.edu)
Genes1
Gene set enrichment analysis of differentially expressed genes in cardiomyocytes isolated from E13.5 hearts of wild-type and mutant mice reveals significant enrichment of MYC targets and, consistent with this finding, we observe increased abundance of MYC in mutant hearts. (nyu.edu)
Expression2
The incidence of linked to expression of an orange pigment with antioxidant invasive GBS disease in nonpregnant adults is growing, in properties ( 6 , 15 ), and these 2 factors act in concert to im- particular in elderly persons and in those with chronic un- pair macrophage-based immune clearance ( 16 ). (cdc.gov)
We generated a dominant-negative sox9b ( dnsox9b ) to inhibit sox9b target gene expression and used the Gal4/UAS system to drive dnsox9b specifically in cardiomyocytes. (nature.com)
Mouse1
Porrello ER, Mahmoud AI, Simpson E et al (2011) Transient regenerative potential of the neonatal mouse heart. (springer.com)
Hearts1
Implanting these electrically immature cardiomyocytes (CMs) into hearts might carry arrhythmogenic risks. (ca.gov)
Development1
Together our studies indicate sox9b transcriptional regulation is necessary for cardiomyocyte development and function. (nature.com)
Recent1
Recent evidence indicates that EGF receptor transactivation by heparin-binding EGF (HB-EGF) contributes to hypertrophic signaling in cardiomyocytes . (pharmaceuticalintelligence.com)
Disease1
The incidence of invasive GBS disease in nonpregnant adults is growing, in particular in elderly persons and in those with chronic underlying conditions (e.g., diabetes mellitus) ( 1 ). (cdc.gov)
Support1
Despite their high degree of diploidy, the Purkinje lineage had no enhanced competence to support regeneration after adult heart infarction. (nyu.edu)