• found in their study that one-fourth of the nurse cells isolated from mice were double-positives for K5 and K8, while the rest of them were positive only for K8. (wikipedia.org)
  • in HY-TCR transgenic mice. (wikipedia.org)
  • The cancer-preventive activity of an extract of Withania somnifera (WS) roots was examined in female transgenic (MMTV/Neu) mice that received a diet containing the extract (750 mg/kg of diet) for 10 months. (nih.gov)
  • Jak3 mediates growth signals through cytokine receptors such as interleukin-2 (IL-2), IL-4, and IL-7, and its deficiency results in autosomal recessive SCID in mice and humans. (rupress.org)
  • Using single-cell RNA sequencing (RNAseq), we studied the development of ProSG, their SG descendants and testicular somatic cells during the perinatal period in mice. (biologists.com)
  • One exception is hu- humanized SCID mice, the use of al oncogenic viruses that are strictly man T-cell lymphotropic virus type 1 surrogate hosts has not proven very species-specific, causing cancer in (HTLV-1): in addition to its ability to useful for defining tumour site con- humans only. (who.int)
  • Inoculation with a high dose strains of LMP1 transgenic mice vide a powerful tool in mechanistic of EBV caused a B-cell lymphopro- were established that express LMP1 studies on the role of individual viral liferative disorder in these mice, under the control of the immunoglob- genes in cancer. (who.int)
  • Gene-targeted mice lacking Vav have a severe defect in positive and negative selection of T cell antigen receptor transgenic thymocytes in vivo, and vav−/− thymocytes are completely resistant to peptide-specific and anti-CD3/anti-CD28-mediated apoptosis. (ox.ac.uk)
  • Here, we characterized the TH system in the retinas of db/db mice and highlighted regulatory processes in MIO-M1 cells. (bvsalud.org)
  • The highest degrees of colocalization were observed in mice harbouring the 20-kb construct, suggesting a large promoter fragment is required to faithfully direct transgene expression in a 5-HT(1A) receptor like pattern. (ox.ac.uk)
  • Class II major histocompatibility complex mutant mice to study the germline bias of T cell antigen receptors. (nationaljewish.org)
  • Metal-specific CD4+ T-cell responses induced by beryllium exposure in HLA-DP2 transgenic mice. (nationaljewish.org)
  • We have studied the transgenic mouse strain, Tyrp-1-TAg, to try to gain insight into possible molecular mechanisms common to pigmented ocular neoplasms occurring spontaneously in the eyes of these mice and human choroidal melanoma. (molvis.org)
  • Using semi-quantitative reverse-transcription polymerase chain reaction (RT-PCR) and western blots experiments, we compared changes in ETS-1 and ETS-2 expression, their protein levels, and the regulation of some of their target gene expressions at different stages of the ocular tumoral progression in the transgenic mouse model, Tyrp-1-TAg, with those in normal eyes from control mice of the same age. (molvis.org)
  • review the heterogeneity of the current models for memory T cells generation and maintenance in humans and mice. (scielo.br)
  • In a very interesting review, P. MARRACK & J. KAPPLER focus on naïve, activated, and memory T cells survival separately, and discuss experiments done in vitro and in mice lacking or containing normal populations of lymphocytes. (scielo.br)
  • Moreover, deletion of p62 did not change the levels of crystallins, histone H3, the mitochondrial import receptor subunit TOM20 homolog, or the abundance of nuclei and nuclear fragments in the lenses of homozygous Cx50D47A mice. (molvis.org)
  • Using Ptpn22 −/− mice we demonstrate that the phosphatase PTPN22 is a highly selective, negative regulator of cDC2 homeostasis, preventing excessive population expansion from as early as 3 weeks of age. (frontiersin.org)
  • Herbach N, Bergmayr M, Göke B, Wolf E, Wanke R. Postnatal development of numbers and mean sizes of pancreatic islets and beta-cells in healthy mice and GIPR(dn) transgenic diabetic mice. (jimdo.com)
  • Herbach N, Goeke B, Schneider M, Hermanns W, Wolf E, Wanke R. Overexpression of a dominant negative GIP receptor in transgenic mice results in disturbed postnatal pancreatic islet and beta-cell development. (jimdo.com)
  • In transgenic mice expressing hen egg lysozyme (HEL) as a neo self-antigen, B cells transgenic for a high affinity B cell receptor (BCR) specific for HEL undergo different fates ( 2 , 3 ). (silverchair.com)
  • We found that mammary epithelium-specific overexpression of CCN5 in transgenic mice leads to an increase in ER-α expression and that this impact of CCN5 is not restricted to the normal cells. (ku.edu)
  • Teh YM, Neuberger MS: The immunoglobulin (Ig)alpha and Igbeta cytoplasmic domains are independently sufficient to signal B cell maturation and activation in transgenic mice. (exbio.cz)
  • We recently provided evidence that the generation of endogenous extracellular vesicles (EVs) engineered for the incorporation of Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV)-2 Nucleocapsid (N) protein induced immunity in the lungs of K18-hACE2 transgenic mice, which then can survive the lethal virus infection. (nature.com)
  • Methods: hTLR-4 negative and positive mice of both sexes (n=6 per group) were exposed to vehicle control (dispersion media, DM) or nickel oxide nanoparticles (NiONP, 48 nm) in one of three doses (2.5, 5, or 20 µg) once by oropharyngeal aspiration (0 d). (cdc.gov)
  • A similar trend was observed with respect to total BAL cell number, which increased to the greatest degree in female hTLR-4 positive mice at 7 d (4.1x increase over DM). (cdc.gov)
  • Unlike in females, the time course of macrophage activation in male mice was consistent between groups, reaching peak values at 14 d (17.7% in hTLR-4 negative males, 22.3% in hTLR-4 positive). (cdc.gov)
  • Conclusions: Overall, hTLR-4 positive mice exhibited a significantly greater degree of biological responsivity to NiONP than same-sex hTLR-4 negative controls. (cdc.gov)
  • We observed a significant increase in IFN-γ-producing CD8 + T cells in bronchoalveolar lavage fluid (BALF) of immunocompetent mice that repeatedly aspirated A. fumigatus conidia in contrast to mice challenged with A. versicolor , a species that is not typically associated with invasive, disseminated disease. (cdc.gov)
  • Airway IFN-γ + CD8 + T-cells were decreased and lung germination was eliminated in mice that aspirated A. fumigatus conidia that were formaldehyde-fixed or heat-inactivated. (cdc.gov)
  • Furthermore, A. fumigatus particles exhibited greater persistence in the lungs of recipient mice when compared to non-viable A. fumigatus or A. versicolor , and this correlated with increased maintenance of airway memory-phenotype CD8 + T cells. (cdc.gov)
  • Analysis of the murine 5-HT receptor gene promoter in vitro and in vivo. (ox.ac.uk)
  • provides an overview of the dynamic in vivo life of NK cells from their development in the bone marrow to their mature cellular responses in the periphery and their ultimate demise, with emphasis on mouse NK cells and viral infections. (scielo.br)
  • While BM-MSCs have high cell-to-cell heterogeneity in gene expression, the cell subtypes that contribute to this heterogeneity in vivo in humans have not been characterized. (ijbs.com)
  • Zebrafish ( Danio rerio ) larvae provide a unique platform to study macrophage-microbe interactions in vivo , from the level of the single cell to the whole organism. (frontiersin.org)
  • Maintaining the homeostasis of the multiple functionally distinct conventional dendritic cells (cDC) subsets that exist in vivo is crucial for regulating immune responses, with changes in numbers sufficient to break immune tolerance. (frontiersin.org)
  • Thus, this study demonstrates that using proteomic methods on our transgenic model can uncover important in vivo APP-interacting proteins that will provide insights into the biology of APP. (jneurosci.org)
  • Thus, this study demonstrates that our transgenic model can uncover important in vivo APP-interacting proteins that will contribute to our understanding of APP processing in in vivo settings. (jneurosci.org)
  • Preliminary results indicate that some tumor antigen-specific T cells may indeed be CD28- and express NK receptors in vivo. (ox.ac.uk)
  • Promoter fragments greater than 4.5 kb in length were active in 5-HT(1A) receptor mRNA positive cells and inactive in 5-HT(1A) receptor mRNA negative cells. (ox.ac.uk)
  • We show via an in vitro binding assay that miR-9a binds to sNPFR1 mRNA in insect cells and to the mammalian orthologue NPY2R in rat insulinoma cells. (nature.com)
  • The extensions of plasma membrane from thymic nurse cells form a cage-like structure, which trap (Hendrix et al. (wikipedia.org)
  • 2010) triple positive T cells, αβTCRlowCD4+CD8+ within the spaces formed by the interlocking of the membrane. (wikipedia.org)
  • We hypothesized lysosome-associated membrane glycoprotein 1 (LAMP1, CD107a) to be a marker for fratricide in TCR transgenic CD8 + T cells. (oncotarget.com)
  • PBP 10, an antibacterial, cell membrane-permeant rhodamine B-conjugated peptide derived from the polyphosphoinositide binding site of gelsolin, interacts selectively with both lipopolysaccharides (LPS) and lipoteichoic acid (LTA), the distinct components of gram-negative and gram-positive bacteria, respectively. (upenn.edu)
  • In this case, ubiquitinated substrates (such as bacterial components or damaged membrane) are recognized by autophagy receptors, including p62 and NDP52, which direct formation of the autophagic membrane around the targeted pathogen ( 18 - 20 ). (frontiersin.org)
  • Here, we demonstrate that developing self-reactive B cells are not always highly sensitive to the deletion mechanisms imposed by membrane-bound self-antigens. (silverchair.com)
  • At low amounts, membrane-bound antigens allow survival of B cells bearing a single high affinity self-reactive B cell receptor (BCR). (silverchair.com)
  • When HEL was expressed in membrane form, HEL-specific B cells were deleted early on ontogeny ( 3 ). (silverchair.com)
  • Based on these results, it was postulated that B cell deletion required BCR cross-linking and could only be induced by membrane bound self-antigens. (silverchair.com)
  • Using this tag, we prepared mild detergent lysates from transgenic mouse brain cortical membrane preparations and isolated a number of previously identified APP-interacting proteins. (jneurosci.org)
  • From studies of APP trafficking and metabolism, the following pathways have emerged: in the first, a fraction of APP molecules residing on the cell surface are processed by ADAM/TACE "sheddases" N-terminal to the ectodomain-transmembrane domain to generate an 83 aa membrane-tethered stub, termed α-CTF. (jneurosci.org)
  • Fuentes-Pananá EM, Bannish G, van der Voort D, King LB, Monroe JG: Ig alpha/Ig beta complexes generate signals for B cell development independent of selective plasma membrane compartmentalization. (exbio.cz)
  • Second, the authors discuss that inability to resolve infection and prevent disease may not be a consequence of the generation of an inadequate number of Th1 cells but of an intrinsic deficiency in macrophage function that prevents these cells from expressing immunity. (scielo.br)
  • Phagocytosis can involve the recognition of pathogen- and damage-associated molecular patterns (PAMPs and DAMPs, respectively) through pattern recognition receptors (PRRs) on the macrophage surface ( 5 , 6 ). (frontiersin.org)
  • 6 SAR156497 7 SP has SAR156497 an autocrine regulation role in macrophage function (8 9 For example in the macrophage cell line P388D1 anti-SP antibody depletion of macrophage-secreted SP from the culture resulted in abrogation of SP-increased IL-1 production (8). (opioid-receptors.com)
  • Rubtsova K, Rubtsov AV, Thurman JM, Mennona JM, Kappler JW and Marrack P. B cells expressing the transcription factor T-bet drive lupus-like immunity. (nationaljewish.org)
  • Rubtsova, K., Rubtsov, A. V., Cancro, M. P. & Marrack , P . Age-Associated B Cells: A T-bet-Dependent Effector with Roles in Protective and Pathogenic Immunity. (nationaljewish.org)
  • The authors also argue that memory B and T cell responses are long-lived in the absence of antigen, but they are modulated and influenced by other infections and that memory B and T cells can either provide protective immunity or contribute to immunopathology on infection with homologous or heterologous viruses. (scielo.br)
  • discuss the role of integrins in T cell- mediated immunity, with a focus on how these receptors participate in lymphocyte recirculation and T cell activation, how antigen stimulation regulates integrin activity, and how integrins define functionally unique subsets of T cells and APCs. (scielo.br)
  • Mechanisms of cell-autonomous immunity are crucial for protection of the host cell cytosol ( 14 ). (frontiersin.org)
  • The multiligand receptors that form the focus of this Perspective series have expectedly diverse functions, often conforming to potential gaps in the host response to invading pathogens that are not effectively manned by adaptive immunity. (jci.org)
  • However, nothing is known about the ability of the N-specific CD8 + T cell immunity in controlling viral replication in the lungs, a major pathogenic signature of severe disease in humans. (nature.com)
  • The virtual absence of Spike-specific cell immunity in lungs of vaccinees is not surprising given that the vaccine is administrated intramuscularly. (nature.com)
  • In fact, it is known that the development of cell immunity in pulmonary tissues is largely independent of the events occurring in both peripheral circulation and distal lymphoid organs. (nature.com)
  • PBDEs and their derivatives, hydroxylated PBDEs (OH-PBDEs), can bind to hormone receptors and impact hormone secretion, transportation, and metabolism, leading to endocrine disruption and the development of various diseases. (bvsalud.org)
  • Patients with triple negative breast cancer (TNBC), where the cancer cells do not express nuclear hormone receptors and human epidermal growth factor receptor 2 (HER2), have worse survival rate compared to the patients with luminal subtypes of cancer. (ku.edu)
  • Vascular endothelial growth factor receptor-2 (VEGFR2) is a member of the VEGF family of receptor tyrosine kinase proteins, which are expressed on both endothelial and tumor cells, are one of the key proteins contributing to cancer development, and are involved in drug resistance. (researchgate.net)
  • This concept implies that olfactory receptor proteins are located in sensory cilia and in the axons. (jneurosci.org)
  • By means of immunohistochemistry and double-labeling studies using transgenic mouse lines as well as Western blot analyses, it was demonstrated that the newly generated antibodies specifically recognized the receptor proteins. (jneurosci.org)
  • To scrutinize the hypothesis that olfactory receptor proteins may also be present in the axonal processes and the nerve terminals, serial sections through the olfactory bulb were probed with the antibodies. (jneurosci.org)
  • These data indicate that olfactory receptor (OR) proteins are indeed present in the axonal processes and nerve terminals of olfactory sensory neurons, thus supporting the notion that ORs may participate in the molecular processes underlying the fasciculation and targeting of olfactory axons. (jneurosci.org)
  • In this study, antibodies were generated against unique epitopes of distinct OR types and used in immunohistochemical experiments to visualize the receptor proteins in whole-mount preparations and tissue sections of the olfactory system. (jneurosci.org)
  • Highly sensitive saturation labeling reveals changes in abundance of cell cycle-associated proteins and redox enzyme variants during oocyte maturation in vitro. (jimdo.com)
  • Here, we show that APP can coimmunoprecipitate NEEP21 from nontransgenic mouse brain and from mammalian cells stably coexpressing both proteins. (jneurosci.org)
  • Background CAR-based cell therapies are hampered by a lack of cell surface target proteins that are also good tumor-specific antigens. (bmj.com)
  • Activated NK cells were transduced with γ-retroviruses directing expression of CIR proteins, soluble IL-15 and a ΔCD19 marker protein or CAR control constructs directed to HLA-G or CD33. (bmj.com)
  • They both are transmembrane proteins with extended cytoplasmic domains containing immunoreceptor tyrosine activation motives (ITAMs), and together with cell surface immunoglobulin they constitute B-cell antigen-specific receptor (BCR). (exbio.cz)
  • Autologous as well as allogeneic CD8 + T cells transduced with tumor antigen specific T cell receptors (TCR) may cause significant tumor lysis upon adoptive transfer. (oncotarget.com)
  • We identified both gene and protein markers for three temporally distinct ProSG cell subsets, including a migratory cell population with a transcriptome distinct from the previously defined T1- and T2-ProSG stages. (biologists.com)
  • Peptide-specific cell death was blocked both by cytochalasin D inhibition of actin polymerization and by inhibition of protein kinase C (PKC). (ox.ac.uk)
  • Detection of T cell responses to a ubiquitous cellular protein in autoimmune disease. (nationaljewish.org)
  • Next, we have shown that CCN5 protein can induce expression of estrogen receptor-α (ER-α) in mammary epithelial cells. (ku.edu)
  • We developed a CD8 + T-cell-based vaccine platform based on intramuscular (i.m.) injection of a DNA vector coding for antigens of interest fused at the C-terminus of a biologically inactive Human Immunodeficiency Virus (HIV)-Type 1 Nef protein (Nef mut ) having an unusually high efficiency of incorporation into EVs. (nature.com)
  • ATTAMAGE-A1.1: Phase I/II study of Autologous CD8+ and CD4+ Transgenic T cells expressing high affinity MAGE-A1-specific T-Cell Receptor (TCR) combined with Atezolizumab in patients with metastatic MAGE-A1 expressing cancer. (fredhutch.org)
  • The cell surfaces of TNCs and their cytoplasmic vacuoles express MHC Class I and MHC Class II antigens. (wikipedia.org)
  • The interaction of these antigens with the developing thymocytes determines whether the thymocytes undergo positive or negative selection. (wikipedia.org)
  • Thymic nurse cells express both MHC Class I and II antigens, and are found in the cortico-medullary junction in addition to the cortex of the thymus. (wikipedia.org)
  • MHC restriction within TNCs Whether the thymocytes undergo positive or negative selection is determined through MHC restriction, which refers to the interaction between the αβTCR (αβ T cell receptor) of the T cells and MHC antigens on the antigen-presenting cells. (wikipedia.org)
  • Only intermediate affinity interaction between the αβTCR of the T cells and MHC antigens in the TNCs results in positive selection. (wikipedia.org)
  • Functionally, cDC1 cross-present exogenous antigens to activate CD8 + T cells and can promote IL-12 dependent Th1 responses ( 1 , 5 - 7 ). (frontiersin.org)
  • Upon i.m. injection of DNA vectors expressing Nef mut -derivatives, nanovesicles containing antigens fused with Nef mut are released by muscle cells, can freely circulate into the body, and can be internalized by antigen-presenting cells (APCs). (nature.com)
  • low quantities of self-antigen induce the selection and accumulation of increased numbers of self-reactive B cells with decreased expression of antigen-specific BCRs. (silverchair.com)
  • Subsequently, in pre-B cells, CD79 heterodimer is associated with lambda5-VpreB surrogate immunoglobulin and later with antigen-specific surface immunoglobulins. (exbio.cz)
  • Animal models for human tumour mental animals is not easy to answer does induce adult T-cell leukaemia/ viruses that make use of animal virus- for these agents, because cancer bi- lymphoma (ATLL), albeit in monkeys es are scarce. (who.int)
  • It has been reported that IL-27 performs an anti-inflammatory function by acting on conventional CD4 + T cells to induce IL-10-producing cells that are implicated in controlling inflammatory responses [ 3 ]. (hindawi.com)
  • These cytokines enhance the immune response and induce pathways leading to pyroptosis, a highly inflammatory type of programmed cell death ( 31 ). (frontiersin.org)
  • Under polarizing inflammatory conditions, cDC2 induce Th2 responses in the lung ( 9 , 10 ), drive Th17 responses through IL-23 secretion ( 11 - 17 ), and initiate SIRPα dependent induction of T follicular helper cells (T FH ) and germinal center (GC) formation ( 18 ). (frontiersin.org)
  • Nagata K, Nakamura T, Kitamura F, Kuramochi S, Taki S, Campbell KS, Karasuyama H: The Ig alpha/Igbeta heterodimer on mu-negative proB cells is competent for transducing signals to induce early B cell differentiation. (exbio.cz)
  • Regulatory vs. inflammatory cytokine T-cell responses to mutated insulin peptides in healthy and type 1 diabetic subjects. (nationaljewish.org)
  • As described below, these diverse situations range from the complications of diabetes and cellular perturbation in amyloidoses to immune and inflammatory responses and tumor cell behavior. (jci.org)
  • Dendritic cells (DCs) are specialized antigen presenting cells that instruct T cell responses through sensing environmental and inflammatory danger signals. (frontiersin.org)
  • Here, we find graded ERK phosphorylation correlating with cell differentiation in patient-derived colorectal cancer organoids with and without KRAS mutations. (researchgate.net)
  • We successfully identified LEPR hi CD45 low BM-MSCs within the CD271 + BM-MNC population, and further codified the BM-MSCs into distinct subpopulations corresponding to the osteogenic, chondrogenic, and adipogenic differentiation trajectories, as well as terminal-stage quiescent cells. (ijbs.com)
  • Cultured oligodendrocyte progenitor cells (OPCs) were incubated with HD ± BMSC-derived conditional medium (BMSC-CM). OPC differentiation was studied by immunostaining and morphometric analysis. (biomedcentral.com)
  • In the groups (both the initiation and the progression) receiving recombinant IL-27 administration, the formation of atherosclerotic plaques was suspended, and the percentage of regulatory T cells (LAP + or Foxp3 + ) in the spleen and peripheral blood was increased. (hindawi.com)
  • Further studies have shown that Th1 cells promote atherosclerosis initiation and progression [ 6 , 7 ]. (hindawi.com)
  • Metastatic triple negative breast cancer (TNBC), urothelial, and non-small cell lung cancer (NSCLC) are solid tumor malignancies with abysmal survival rates following progression on initial therapies. (fredhutch.org)
  • therefore, depending on the nature of the target (valence, abundance, location, metabolism, and its intrinsic biology), antibody-target and off-target mediated mechanisms of disposition and clearance will be affected by interactions with the Fc Gamma family of receptors. (pegsummit.com)
  • Cultured bovine retinal endothelial cells (BREC) and bovine retinal pericytes (BRP) were incubated with Ang II (100 nmol/l) or Ang II+candesartan (1 μmol/l). (diabetesjournals.org)
  • Efficient recognition of foreign pathogens by T cells requires adhesive interactions between T cells and other cell types, such as endothelial cells and antigen-presenting cells (APCs), and with components of the extracellular matrix. (scielo.br)
  • IL-27 binds to IL-27ra and gp-130 complex, which is expressed on multiple cell types, including T lymphocytes [ 2 ]. (hindawi.com)
  • Our studies show that after being secreted into the extracellular matrix, CCN5 binds to the α6β1 integrins of the cells leading to inhibition of the PI3K-AKT signaling pathway. (ku.edu)
  • A Rapid Method to Characterize Mouse IgG Antibodies and Isolate Native Antigen Binding IgG B Cell Hybridomas. (nationaljewish.org)
  • To approach this critical issue, antibodies were generated against two peptides, one derived from olfactory receptor mOR256-17, one derived from the "mOR37" subfamily. (jneurosci.org)
  • In the B cell system protective memory is mediated by plasma cells that secrete antibodies, whereas reactive memory is mediated by memory B cells that proliferate and differentiate to plasma cells in response to secondary antigenic stimulation. (scielo.br)
  • When HEL was expressed in a soluble form, HEL-specific B cells were not deleted, but became unable to proliferate or secrete HEL-specific antibodies ( 2 ). (silverchair.com)
  • Two parallel immunoblots had been operate from same cell lysates using antibodies against the Thr-183/Tyr-185, Thr-180/Tyr-182, and Thr-202/Tyr-204 phosphorylated (ie, turned on) JNK (p-JNK), p38 (p-p38), and Erk1/2 (p-Erk1/2) and against total JNK, p38, and Erk1/2. (californiaehealth.org)
  • To further analyse the promoter of this receptor gene, additional upstream sequence information extending to -5.5 kb of the murine htr1a was generated and promoter fragments extending to -20 kb were analysed for activity in cell culture and transgenic animals. (ox.ac.uk)
  • Separation of murine CD79b positive lymphocytes (red-filled) from CD79b negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-mouse CD79b (HM79) purified antibody (concentration in sample 1.67 μg/ml, GaArH APC). (exbio.cz)
  • Therefore, murine airway CD8 + T cell-responses to aspiration of Aspergillus conidia may be mediated in part by the ability of conidia to germinate in the host lung tissue. (cdc.gov)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • Olfactory receptors are supposed to act not only as molecular sensors for odorants but also as cell recognition molecules guiding the axons of olfactory neurons to their appropriate glomerulus in the olfactory bulb. (jneurosci.org)
  • Other unusual features of the receptor include its ability to engage classes of molecules, rather than individual ligands, and its enhanced surface expression in environments rich in RAGE ligands. (jci.org)
  • MW) chemicals interact with host cells and molecules and In our daily life, we are confronted with a plethora of natural influence biologic processes such as signal transduction. (cdc.gov)
  • Importantly, recent studies have shown that the importance of type 17 T helper cells (Th17 cells) and regulatory T cells (Tregs) in the pathogenesis of various immune disorders, particularly in atherosclerosis. (hindawi.com)
  • In MIO-M1 cells, a feedback regulatory circuit was evidenced whereby miR-133-3p triggered the post-transcriptional repression of DIO3 in a T3-dependent manner, while high glucose (HG) led to DIO3 upregulation through a nuclear factor erythroid 2-related factor 2-hypoxia-inducible factor-1 pathway. (bvsalud.org)
  • Apart from purging the T cell repertoire from auto-reactive T cells via negative selection in the thymus dominant tolerance exerted by regulatory T cells plays a major role in tolerance imposition and maintenance. (epa.gov)
  • Among the various regulatory/suppressive cells hitherto described, CD4+CD25+ regulatory T cells (Treg) and interleukin-10 producing T regulatory 1 (Tr1) cells have been studied in most detail and are the subject of most articles in this issue. (epa.gov)
  • Treg, also called "natural" regulatory T cells, will be traced from their intra-thymic origin to the site of their action in peripheral lymphoid organs and tissues. (epa.gov)
  • Regulatory T cells, however also control infections, allergies and tolerance to transplanted tissues and this requires their induction in the periphery under conditions which are not yet fully understood. (epa.gov)
  • Based on these data and the known activated phenotype of CD28- T cells, we propose that the CD28- CD8+ T cell pool represents or contains the currently active CTL population, and that the frequent expression of NK receptors reflects regulatory mechanisms modulating the extent of CTL effector function. (ox.ac.uk)
  • Treg, regulatory T cell. (cdc.gov)
  • In cocultures, CIR-NK cells displayed potent serial killing activity of both cell lines accompanied by secretion of IFN-γ at E:T ratios of 1:10 and 1:20. (bmj.com)
  • The secretion of NGF by BMSC and activation of NGF receptor was found important for suppression of TNFα production in OPCs. (biomedcentral.com)
  • Using colocalization studies we analysed the degree of concurrence of transgenic and endogenous htr1a expression brought about by these three different constructs. (ox.ac.uk)
  • Further, AML cell lines MOLM13 and Kasumi1 express low endogenous levels of HLA-G1 and G5. (bmj.com)
  • Negative selection has been proposed to occur when the αβTCR in developing T cells interact with MHC present on antigen-presenting cells like dendritic cells and macrophages with strong affinities, which then leads T cells down the apoptotic pathway inside the TNCs. (wikipedia.org)
  • that is histopathological y very simi- T cel s, B cel s, natural kil er cel s, LMP1 of EBV can transform ro- lar to that caused by hepatitis B vi- macrophages, and dendritic cells, dent fibroblasts and is expressed rus (HBV) in humans, but it does so and this humanized mouse model in most of the human cancers as- through a different mechanism. (who.int)
  • The expression level of the 5-HT(1A) receptor gene (htr1a) in the central nervous system (CNS) is implicated in the aetiology and treatment of anxiety disorders and depression. (ox.ac.uk)
  • This phase I/II trial studies the side effects of gene-modified immune cells (FH-MCVA2TCR) and to see how well they work in treating patients with Merkel cell cancer that has spread to other parts of the body (metastatic) or that cannot be removed by surgery (unresectable). (fredhutch.org)
  • Placing a gene that has been created in the laboratory into immune cells may improve the body's ability to fight Merkel cell cancer. (fredhutch.org)
  • ERK levels are generally higher in cancer cells adjacent to stromal cells at the invasive front and lower in more central areas of cancer specimens. (researchgate.net)
  • Non-small cell lung cancer (NSCLC) represents most of the diagnoses of lung cancer. (researchgate.net)
  • genic models are inadequate for number of activated CD8-positive T LMP1 was strongly expressed in the understanding the cancer etiology in cells increased considerably in the lymphoma tissues but was hardly the context of natural viral infection. (who.int)
  • Trevino V, Tadesse MG, Vannucci M, Al-Shahrour F, Antczak P, Durant S, Bikfalvi A, Dopazo J, Campbell MJ, Falciani F. (2011) Analysis of normal-tumour tissue interaction in tumours: prediction of prostate cancer features from the molecular profile of adjacent normal cells. (u-bordeaux.fr)
  • Identification of shared TCR sequences from T cells in human breast cancer using emulsion RT-PCR. (nationaljewish.org)
  • We have developed an engineered autologous T cell therapy targeting cancer testes antigen MAGE-A1. (fredhutch.org)
  • In a phase I trial, we seek to determine the safety and potential efficacy of this T cell therapy in addition to an FDA approved therapeutic standard anti-PD-L1 therapeutic, atezolizumab, for patients with advanced triple negative breast cancer, urothelial, or non-small cell lung cancers whose tumors express high level of MAGE-A1 and have become resistant to PD-L1 axis treatments. (fredhutch.org)
  • We have developed an engineered autologous T cell therapy targeting mesothelin, an antigen overexpressed by pancreatic cancer cells. (fredhutch.org)
  • Durmus Lab develops novel, label-free, and magnetic levitation-based microfluidic chips for the measurement of cancer cell densities. (stanford.edu)
  • Lack of ER-α expression in TNBC cells or loss of ER-α activation after endocrine treatment of luminal cancers makes these breast cancer cells resistant to tamoxifen and other endocrine therapies. (ku.edu)
  • In addition, it generates cancer stem cells (CSCs) that have the ability, following dissemination, to serve as founders of new metastatic colonies. (pegsummit.com)
  • In a phase I trial, we seek to determine the safety and preliminary antitumor efficacy of this T cell therapy in patients with metastatic pancreatic ductal adenocarcinomas whose tumors express mesothelin and have progressed on prior chemotherapy. (fredhutch.org)
  • Dahlhoff M, Grzech M, Habermann FA, Wolf E, Schneider MR. A transgenic mouse line expressing cre recombinase in pancreatic β-cells. (jimdo.com)
  • Grzech M, Dahlhoff M, Herbach N, Habermann FA, Renner-Müller I, Wanke R, Flaswinkel H, Wolf E, Schneider MR. Specific transgene expression in mouse pancreatic beta-cells under the control of the porcine insulin promoter. (jimdo.com)
  • Here, we have shown that Cysteine-rich 61-Connective Tissue Growth Factor-nephroblastoma-overexpressed 5 (CCN5) induces growth arrest of TNBC cells in-vitro and in xenograft tumors. (ku.edu)
  • CCN5 treatment leads to an expression of functional ER-α in the TNBC cells, both in-vitro and in xenograft models, and sensitizes these cells to tamoxifen, commonly used for endocrine therapies. (ku.edu)
  • Anti-tumor efficacy of CIR-NK cells in vitro. (bmj.com)
  • Platforms include Epibase™ In Silico for the prediction of T cell epitopes and Epibase™ In Vitro for the assessment of T cell and B cell responses in human donor PBMC. (pegsummit.com)
  • Transgenic mouse models pro- fection. (who.int)
  • Monographs , transgenic mouse tients. (who.int)
  • In normal control adult mouse eyes, ETS-1 was mostly present in the nuclei of all neuroretinal layers whereas ETS-2 was mostly localized in the cytosol of the cell bodies of these layers with a smaller amount present in the nuclei. (molvis.org)
  • On serial sections through the olfactory bulb of mOR37-transgenic mouse lines, double-labeling experiments demonstrated that distinct immunoreactive glomeruli corresponded to glomeruli that were targeted by neurons expressing a particular member of the mOR37 receptor subfamily. (jneurosci.org)
  • The Armenian hamster monoclonal antibody HM79 recognizes an extracellular epitope of mouse CD79b (CD79 beta, Ig beta), a component of B cell receptor (BCR) complex. (exbio.cz)
  • Significant SP immunoreactivity continues to be seen in HIV gp120 transgenic mouse human brain vessels in comparison to nontransgenic mouse human brain vessels recommending that SP is certainly involved with HIV gp120-induced adjustments in the vascular element of the blood-brain hurdle (12). (opioid-receptors.com)
  • Pulmonary toxicity of nickel oxide nanoparticles in a transgenic mouse model expressing humanized Toll-like receptor-4 (TLR-4). (cdc.gov)
  • The directed expression of SV40 T antigen has led to the development of several important transgenic models with spontaneous epithelial tumor formation. (molvis.org)
  • Recent studies indicate that memory T lymphocytes contain distinct populations of central memory (T CM cells) and effector memory (T EM ) cells characterized by distinct homing capacity and effector function. (scielo.br)
  • CD28-negative cytolytic effector T cells frequently express NK receptors and are present at variable proportions in circulating lymphocytes from healthy donors and melanoma patients. (ox.ac.uk)
  • In humans, NK receptors are expressed by natural killer cells and some T cells, the latter of which are preferentially alphabetaTCR+ CD8+ cytolytic T lymphocytes (CTL). (ox.ac.uk)
  • Atherosclerosis, which is characterized by chronic inflammation in the arterial wall, is driven by immune cells and cytokines. (hindawi.com)
  • Many studies have indicated that numerous immune cells promote chronic inflammation and progressive plaque growth [ 4 ]. (hindawi.com)
  • We therefore postulated that SP and its receptors are actively involved in HIV contamination of human immune cells such as MDM. (opioid-receptors.com)
  • Bone marrow-derived mesenchymal stem cells (BM-MSCs) are multipotent stromal cells that have a critical role in the maintenance of skeletal tissues such as bone, cartilage, and the fat in bone marrow. (ijbs.com)
  • Demyelinating conditions are difficult to treat and stem cell therapy using bone marrow mesenchymal stem cells (BMSCs) is a promising novel strategy. (biomedcentral.com)
  • Kouro T, Nagata K, Takaki S, Nisitani S, Hirano M, Wahl MI, Witte ON, Karasuyama H, Takatsu K: Bruton's tyrosine kinase is required for signaling the CD79b-mediated pro-B to pre-B cell transition. (exbio.cz)
  • Rubtsova, K, Rubtsov, AV Halemano K, Sam LX, Kappler JW , Sanitago ML and Marrack P . T cell production of IFNg in response to TLR7/IL12 stimulates optimal B cell responses to viruses. (nationaljewish.org)
  • As a consequence, cDC2 dependent CD4 + T cell proliferation and T follicular helper cell responses are increased. (frontiersin.org)
  • Collectively, our data demonstrate that PTPN22 controls cDC2 homeostasis, which in turn ensures appropriate cDC2-dependent T cell responses under antigenic challenge. (frontiersin.org)
  • Meanwhile IL-27 exerts proinflammatory effects by promoting Th1, CD8, natural killer (NK), T follicular helper (Tfh), and B cell proliferation/functions and by inhibiting Treg and Th2 cell generation in parasites infection [ 4 ]. (hindawi.com)
  • Antiviral CD8 + T Cells Restricted by Human Leukocyte Antigen Class II Exist during Natural HIV Infection and Exhibit Clonal Expansion. (nationaljewish.org)
  • IRGs and GBPs belong to a family of GTPases that confer host cell resistance during infection by pathogens ( 22 - 24 ). (frontiersin.org)
  • The research conducted by central investigators is quite broad, ranging from cell and molecular biology to tissue engineering, biophysics and nanobiology/medicine. (upenn.edu)
  • Methods CD56 + NK cells were selected from PBMCs, incubated with IL-15 and activated with a feeder free cocktail of an immobilized cytokine and activating ligand. (bmj.com)
  • In comparative experiments, T cells from PBMCs were transduced to produce CIR-T cells by standard methods. (bmj.com)
  • Materials and Methods Cells. (opioid-receptors.com)
  • In an effort to elucidate the mechanism of bacterial killing by PBP 10, we compared its effects on artificial lipid bilayers and eukaryotic cell membranes with the actions of the mellitin, magainin II, and LL37 peptides. (upenn.edu)
  • We also investigated the effects of these peptides on platelets and red blood cells (RBCs). (upenn.edu)
  • This finding suggests that asymmetrical distribution of phospholipids in the external membranes of eukaryotic cells may represent an important factor in determining the specificity of antibacterial peptides for targeting bacteria rather than eukaryotic cells. (upenn.edu)
  • Together, the data suggest that, in the early phases of DR, a DIO3-driven LT3S may be protective against retinal stress, while, in the chronic phase, it not only fails to limit HG-induced damage, but also increases cell vulnerability likely due to persistent mitochondrial dysfunction. (bvsalud.org)
  • The FHA cycle is active inside the cell, detoxifying superoxide anion radicals (O2·-), which are mainly generated as a result of so-called 'electron leakage' to triplet oxygen (O2) in photosynthetic, mitochondrial, peroxisomal, and probably other electron transport chains (Ozyigit et al. (deepdyve.com)
  • After therapy, the incidence of pleomorphic adenomas, mucoepidermoid carcinomas, and squamous cell carcinomas is increased. (medscape.com)
  • These include a research center in Cell Studies of Pulmonary Artery Hypertension, and a Penn Center for Molecular Discovery. (upenn.edu)
  • Correspondence with low molecular weight chemicals that results in the priming of chemical-specific, Prof. Dr. Stefan F. Martin, Allergy Research skin-homing CD8+ Tc1/Tc17 and CD4+ Th1/Th17 cells. (cdc.gov)
  • Labeling indices for Ki67 and proliferating cell nuclear antigen marker in mammary carcinomas of the treated group were 35% and 30% lower, respectively, than those of the corresponding control group. (nih.gov)
  • Also, we found that the CCN5-induced PI3K-AKT inactivation leads to stabilization and nuclear accumulation of P27KIP1 resulting in cell cycle arrest of TNBC cells. (ku.edu)
  • We identified HLA-A*02:01/peptide-restricted T cells directed against ADRB3 295 . (oncotarget.com)
  • After TCR identification, we generated HLA-A*02:01/peptide restricted TCR transgenic T cells by retroviral transduction and tested T cell expansion rates as well as A*02:01/peptide recognition and ES killing in ELISpot and xCELLigence assays. (oncotarget.com)
  • Comparison of peptide motive binding affinities revealed extended fratricide among ADRB3 295 specific TCR transgenic T cells in contrast to CHM1 319 . (oncotarget.com)
  • Autoreactive T cells specific for insulin B:11-23 recognize a low-affinity peptide register in human subjects with autoimmune diabetes. (nationaljewish.org)
  • Mechanistically, PTPN22 mediates cDC2 homeostasis in a cell intrinsic manner by restricting cDC2 proliferation. (frontiersin.org)
  • In this study we analyzed the expression of nine NK receptors (p58.1, p58.2, p70, p140, ILT2, NKRP1A, ZIN176, CD94 and CD94/NKG2A) in PBL from both healthy donors and melanoma patients. (ox.ac.uk)
  • HLA-G directs immunosuppression by binding to ILT receptors (ILT2 in lymphoid cells and ILT4 in myeloid cells) via ITIM signaling domains. (bmj.com)
  • A) Schematic depiction of a CIT comprising the extracellular D1 and D2 domains of ILT2, a stalk and transmembrane domain derived from CD8-α and a replacement of the ITIM domains of ILT2 with engineered signalling domains driving NK cell activation. (bmj.com)
  • B,C) 7-day Co-culture of Kasumi1-GFPffluc (B) or Molm13-GFPffluc AML cells with mock-transduced (Mock) NK cells or NK cells transduced to express CIRs containing the D1-D4 extracellular domains (CIR3) or only the D1-D2 domains (CIR4) from ILT2 or ILT4. (bmj.com)
  • Targeting classic Tregs is limited by the detection of Foxp3 and surface Treg markers on activated cells. (hindawi.com)
  • They conclude that although armed with highly distinct effector functions, there are many parallels between the T cell and B cell components of the immune response and their conversion into memory. (scielo.br)
  • Although initially thought to be involved only in positive selection, thymic nurse cells have now been discovered to facilitate negative selection of thymocytes as well. (wikipedia.org)
  • Negative selection refers to the degradation of thymocytes, and has been found to occur through the help of lysosomes. (wikipedia.org)
  • If the internalized thymocytes are selected for negative selection, vacuoles containing the thymocytes move closer to the area with lysosomes and eventually fuse with the lysosomes. (wikipedia.org)
  • Also, almost 90% of all thymocytes extracted from the female TNCs were found to be double positives (CD4+CD8+), whereas no such phenotype was present within the male thymic nurse cells. (wikipedia.org)
  • Thus, since not all thymocytes internalized by TNCs went through apoptotic pathways, this was used to conclude that thymic nurse cells are involved in MHC restriction process. (wikipedia.org)
  • Thymocytes uptake The thymic cortical cells take up early thymocytes migrating from the bone marrow to the thymus and form the thymocyte-TNC complexes. (wikipedia.org)
  • These results indicate that the root extract reduced the number of mammary carcinomas that developed and reduced the rate of cell division in the carcinomas. (nih.gov)
  • Excretory stem cells give rise to squamous cell and mucoepidermoid carcinomas, while intercalated stem cells give rise to pleomorphic adenomas, oncocytomas, adenoid cystic carcinomas, adenocarcinomas, and acinic cell carcinomas. (medscape.com)
  • Squamous cell carcinomas arise from excretory duct cells, pleomorphic adenomas arise from the intercalated duct cells, oncocytomas arise from the striated duct cells, and acinic cell carcinomas arise from acinar cells. (medscape.com)
  • We also molecularly defined the development of Sertoli, Leydig and peritubular myoid cells during the perinatal period, allowing us to identify candidate signaling pathways acting between somatic and germ cells in a stage-specific manner during the perinatal period. (biologists.com)
  • Results T and NK cells were efficiently and stably transduced by the CIR constructs. (bmj.com)
  • Autocrine regulation of SP also has been suggested in other cell types (10-12). (opioid-receptors.com)
  • We further postulated that by interrupting the SP autocrine loop SP antagonists inhibit HIV contamination of these cells. (opioid-receptors.com)