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  • Oct4
  • We demonstrated that, similar to human development but in contrast to mouse development, OCT4 is required for maintaining NANOG-positive epiblast cells in the inner cell mass of blastocysts. (pnas.org)
  • In contrast, NANOG was absent or very faint in the ICM of OCT4 KO blastocysts, and no cells expressing exclusively NANOG were observed. (pnas.org)
  • This mimics findings in OCT4-deficient human blastocysts but is in sharp contrast to Oct4 -null mouse blastocysts, where NANOG persists and PrE development fails. (pnas.org)
  • Elevated Nanog expression from transgene constructs is sufficient for clonal expansion of ES cells, bypassing Stat3 and maintaining Oct4 levels. (nih.gov)
  • The transcriptional factor Nanog functions in maintaining pluripotency in cooperation with other key genes such as Oct4. (allelebiotech.com)
  • expression
  • Nanog expression in mouse germ cell development. (abnova.com)
  • It has been suggested that Nanog is primarily required for the proper formation of the extraembryonic yolk syncytial layer (YSL) and only indirectly regulates gene expression in embryonic cells. (biologists.org)
  • In the absence of Nanog, YSL formation and epiboly are abnormal, embryonic tissue detaches from the yolk, and the expression of dozens of YSL and embryonic genes is reduced. (biologists.org)
  • A population of mouse embryonic stem (ES) cells is characterized by a distribution of Nanog, a gene whose expression is associated with the degree of pluripotency. (biomedsearch.com)
  • In this study, we used the HepG2 cell line and found that metformin/AICAR downregulated NANOG expression with decreased cell viability and enhanced chemosensitivity to 5-fluorouracil (5-FU). (springer.com)
  • NANOG and FLK1 expression are increased in response to WNT3A stimulation. (ahajournals.org)
  • We propose that NANOG binding to these sites activate the FLK1 -promoter, thereby its expression in a subset of ECs. (ahajournals.org)
  • These results indicate that NANOG transcriptionally regulates FLK1 expression leading to a role in controlling the proliferative and angiogenic activities of endothelial cells. (ahajournals.org)
  • We show that inhibiting the expression of AlkB homolog 5 (ALKBH5), which demethylates m 6 A, or the hypoxia-inducible factors (HIFs) HIF-1α and HIF-2α, which activate ALKBH5 gene transcription in hypoxic breast cancer cells, is an effective strategy to decrease NANOG expression and target BCSCs in vivo. (pnas.org)
  • This study is focused on the analysis of correlation between the clinicopathological features of OSCCs and the immunohistochemical (IHC) expression patterns of MIDKINE (MK) and NANOG. (mdpi.com)
  • MK and NANOG showed significantly similar IHC expression patterns: both demonstrated enhanced expression in histologically high-grade and clinically late-stage OSCCs. (mdpi.com)
  • The enhanced expression of MK and NANOG was associated with lower overall survival rates. (mdpi.com)
  • In conclusion, enhanced IHC expression patterns of MK and NANOG in OSCC patients was significantly associated with lower overall survival rates and unfavorable clinicopathological features. (mdpi.com)
  • These results demonstrate that analysis of IHC expression patterns of MK and NANOG in pretreatment biopsy specimens during the work-up period can provide a more definitive prognosis prediction for each OSCC patient that can help clinicians to develop a more precise individual treatment modality. (mdpi.com)
  • Although latent in normal somatic cells, aberrant expression of Nanog has been reported in many types of human cancers. (aacrjournals.org)
  • Importantly, the expression levels of Nanog are often positively correlated with treatment resistance and poor survival of cancer patients. (aacrjournals.org)
  • Various studies have shown that upregulation of Nanog expression enhances the tumorigenicity both in vivo and in vitro whereas repression or ablation of Nanog inhibits tumor initiation. (aacrjournals.org)
  • The results of our study support the possibility of screening for Nanog expression as a prognostic tool. (aacrjournals.org)
  • The expression of the Nanog orthologue axNanog is required to establish pluripotency during axolotl development and has a conserved role interacting with axSMAD2 and DPY30 to deposit H3K4me3 through COMPASS. (nottingham.ac.uk)
  • regulator
  • Additionally, the researchers showed that Nanog activated the central regulator of muscle formation, serum response factor (SRF), suggesting that the same results may be applicable for skeletal, cardiac and other muscle types. (eurekalert.org)
  • Nanog, most likely, acts as an intracellular regulator, that helps maintain pluripotency and self renewal via a STAT3-independent pathway. (reliatech.de)
  • Here we provide data to support a role for STK33 (Serine Threonine Kinase 33) as a novel regulator of Nanog and as a potential therapeutic target. (aacrjournals.org)
  • Moreover
  • Moreover, BBI-503 (Amcasertib), a first-in-class cancer stemness inhibitor, potently inhibits STK33, which led to inhibition of phosphorylation of Nanog. (aacrjournals.org)
  • pathways
  • In particular, the addition of Chiron and PD03, inhibitors for the ERK and GSK3 signalling pathways, induces a high level of Nanog. (biomedsearch.com)
  • primary
  • Sixty-two primary OSCC patients were selected and their pretreatment biopsy specimens were immunohistochemically analyzed for the MK and NANOG proteins. (mdpi.com)
  • Cell
  • Using a cell line with a fluorescence tag for Nanog enables measurements of the distribution of Nanog in an ES cell culture in a stationary state or after a perturbation. (biomedsearch.com)
  • By applying the model to our experimental data, we infer the existence of three stable steady states for Nanog levels, which are the same in all the different conditions of the cell-culture medium. (biomedsearch.com)
  • Reporter
  • Insertion of the NANOG 3′-UTR into a luciferase reporter gene led to regulation of luciferase activity by O 2 , HIFs, and ALKBH5, which was lost upon mutation of the methylated residue. (pnas.org)
  • mouse
  • Synthetic peptide derived from residues 250 to the C-terminus of Mouse Nanog. (abcam.com)
  • Nanog regulation in a mouse model is currently under way to confirm our results in vivo. (aacrjournals.org)