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  • dehydrogenase
  • The reoxidation of NADH generated in reactions within the mitochondrial matrix of Saccharomyces cerevisiae is catalyzed by an NADH dehydrogenase designated Ndi1p (C. A. M. Marres, S. de Vries, and L. A. Grivell, Eur. (uniprot.org)
  • Disruption of the gene designated NDH1 results in a threefold reduction in total mitochondrial NADH dehydrogenase activity in cells cultivated with glucose and in a fourfold reduction in the respiration of isolated mitochondria with NADH as the substrate. (uniprot.org)
  • Thus, Ndh1p appears to be a mitochondrial dehydrogenase capable of using exogenous NADH. (uniprot.org)
  • Growth phenotype analyses suggest that the external NADH dehydrogenase activity of Ndh1p is important for optimum cellular growth with a number of nonfermentable carbon sources, including ethanol. (uniprot.org)
  • Codisruption of NDH1 and genes encoding malate dehydrogenases essentially eliminates growth on nonfermentable carbon sources, suggesting that the external mitochondrial NADH dehydrogenase and the malate-aspartate shuttle may both contribute to reoxidation of cytosolic NADH under these growth conditions. (uniprot.org)
  • NADH Dehydrogenase subunit 6 (MTND6) is 1 of the 7 mitochondrial DNA (mtDNA) encoded subunits (MTND1, MTND2, MTND3, MTND4L, MTND4, MTND5, MTND6) included among the approximately 41 polypeptides of respiratory Complex I. Complex I accepts electrons from NADH, transfers them to ubiquinone (Coenzyme Q10), and uses the energy released to pump protons across the mitochondria inner membrane. (abcam.cn)
  • mitochondrial
  • A role for N-myristoylation in protein targeting: NADH-cytochrome b5 reductase requires myristic acid for association with outer mitochondrial but not ER membranes. (rupress.org)
  • The mammalian myristoylated flavoenzyme NADH-cytochrome b5 reductase is integrated into ER and mitochondrial outer membranes via an anchor containing a stretch of 14 uncharged amino acids downstream to the NH2-terminal myristoylate glycine. (rupress.org)
  • inner membrane
  • In this process, the complex translocates four protons across the inner membrane per molecule of oxidized NADH , helping to build the electrochemical potential used to produce ATP . (princeton.edu)
  • The reaction catalyzed by complex I is: NADH + H+ + CoQ + 4H+in→ NAD+ + CoQH2 + 4H+out In this process, the complex translocates four protons across the inner membrane per molecule of oxidized NADH, helping to build the electrochemical potential difference used to produce ATP. (wikipedia.org)
  • iron-sulfur
  • The high reduction potential of the N2 cluster and the relative proximity of the other clusters in the chain enable efficient electron transfer over long distance in the protein (with transfer rates from NADH to N2 iron-sulfur cluster of about 100 μs). (wikipedia.org)
  • hydrogen peroxide
  • The NADH peroxidase from Enterococcus faecalis is unique in that it utilizes the Cys42 thiol/sulfenic acid (-SH/-SOH) redox couple in the heterolytic cleavage of the peroxide bond to catalyze the two-electron reduction of hydrogen peroxide to water. (wikipedia.org)
  • NADH eliminates potentially toxic hydrogen peroxide under aerobic growth conditions and represents an enzymatic defense available against H2O2-mediated oxidative stress. (wikipedia.org)
  • redox
  • All redox reactions take place in the extramembranous portion of NADH dehydrogenase. (princeton.edu)
  • The structure is L-shaped with a long, hydrophobic transmembrane domain and a hydrophilic domain for the peripheral arm that includes all the known redox centers and the NADH binding site. (wikipedia.org)
  • initially
  • Initially I am just trying to do a really basic titration of NADH from 0uM, upto 250uM to investigate the effect of NADH concentration on absorbance, using the assay buffer alone as a control. (protocol-online.org)
  • 10mg
  • I have taken only 10mg NADH with ribose obtaining high energy, mental clarity and some of my best days. (webmd.com)
  • Escherichia
  • Escherichia coli complex I (NADH dehydrogenase) is capable of proton translocation in the same direction to the established Δψ, showing that in the tested conditions, the coupling ion is H+. (wikipedia.org)
  • concentrations
  • At suboptimal H2O2 concentrations and concentrations of NADH that are saturating, NADH inhibits the peroxidase activity of the NADH peroxidase by converting the enzyme to an unstable intermediate. (wikipedia.org)
  • The prior PhD student had been using similar concentrations and getting results, but didnt actually do the NADH titration, which makes me wonder if those conclusions are valid, as NADH absorbance is the measured variable and if this measurement may not be reliable (at least according to my experiements). (protocol-online.org)
  • oxidative
  • The actual function of NADH peroxidases and oxidases in plants is still unclear, but they could act in early signaling of oxidative stress through producing H2O2. (wikipedia.org)