AnatomyOrganismsChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsInformation Science
Transcription FactorsMEF2 Transcription FactorsTranscription, GeneticDNA-Binding ProteinsPromoter Regions, GeneticSp1 Transcription FactorGene Expression RegulationMolecular Sequence DataBase SequenceTranscriptional ActivationBinding SitesTrans-ActivatorsMyogenic Regulatory FactorsBasic Helix-Loop-Helix Transcription FactorsRNA, MessengerProtein BindingNuclear ProteinsTranscription Factor AP-1Repressor ProteinsCell LineGene Expression Regulation, DevelopmentalForkhead Transcription FactorsHomeodomain ProteinsSignal TransductionAmino Acid SequenceDNABasic-Leucine Zipper Transcription FactorsMutationTranscription Factor AP-2Cell NucleusCell DifferentiationTransfectionKruppel-Like Transcription FactorsTranscription Factors, TFIIGenes, ReporterChromatin ImmunoprecipitationGATA4 Transcription FactorHeLa CellsCells, CulturedYY1 Transcription FactorSTAT3 Transcription FactorTranscription Factor TFIIDNFATC Transcription FactorsActivating Transcription Factor 3Reverse Transcriptase Polymerase Chain ReactionElectrophoretic Mobility Shift AssaySp3 Transcription FactorNF-kappa BEnhancer Elements, GeneticTranscription Initiation SiteZinc FingersPaired Box Transcription FactorsActivating Transcription Factor 2Transcription Factor TFIIBRegulatory Sequences, Nucleic AcidGene Expression ProfilingE2F1 Transcription FactorRecombinant Fusion ProteinsCloning, MolecularRNA Polymerase IIPlasmidsBasic Helix-Loop-Helix Leucine Zipper Transcription FactorsGene ExpressionGATA3 Transcription FactorGATA1 Transcription FactorGATA2 Transcription FactorGene Expression Regulation, FungalTCF Transcription FactorsGATA Transcription FactorsLuciferasesProtein Structure, TertiaryMicrophthalmia-Associated Transcription FactorMADS Domain ProteinsPhosphorylationSTAT1 Transcription FactorActivating Transcription FactorsCell Line, TumorHelix-Loop-Helix MotifsTranscription Factor RelAE2F Transcription FactorsChromatinOligonucleotide Array Sequence AnalysisSaccharomyces cerevisiaeModels, BiologicalSequence Homology, Amino AcidSaccharomyces cerevisiae ProteinsGene Expression Regulation, PlantMice, KnockoutCyclic AMP Response Element-Binding ProteinGATA6 Transcription FactorActivating Transcription Factor 4Transcription Factor 7-Like 1 ProteinActivating Transcription Factor 1Blotting, WesternProto-Oncogene ProteinsTranscription Factor TFIIIAProto-Oncogene Proteins c-junMice, TransgenicDown-RegulationDrosophila Proteins
ProteinsEnhancer factorMyocyteMyogenesisMyoDTranscriptional regulationCardiacRegulatoryMyosinMEF2CC2C12ProteinEmbryonicMechanismsExpressionMuscleCoordinateMyoblastsPromoterDifferentiationCellIdentificationBasicAttenuated myogenic differentiationGenesSkeletal muscle2002TranscriptionalEpigeneticGene encodesChromatinRegulateMolecular regulationGenomeMuscleActivationMiceResponse
Proteins5
  • Within this conserved region is a consensus site for myocyte enhancer factor 2 (MEF2) proteins that we show is bound efficiently by MEF2 and is required for transgene expression in all three muscle lineages in vivo. (ox.ac.uk)
  • MSY3 (MSY4, YB-2, CSDA, dbpA, ZONAB) is a member of the cold shock domain (CSD) family of proteins also known as Y-box proteins, which are evolutionarily conserved proteins that function as transcription factors and regulators of RNA metabolism and protein synthesis. (biomedcentral.com)
  • Tamir, Y., and Bengal E . (2000) Phosphoinositide 3-kinase induces the transcriptional activity of MEF2 proteins during muscle differentiation . (technion.ac.il)
  • A hallmark of events observed during segmentation was the induction of multiple transcription factors, including a large group of homeobox proteins in pace with decay of a large fraction of maternal transcripts. (biomedcentral.com)
  • Regulatory proteins, namely the Transcription Factors (TFs), bind in a sequence-specific manner to TFBSs to activate or suppress gene transcription (gene expression). (biomedcentral.com)
Enhancer factor1
  • Myocyte-specific enhancer factor 2C also known as MADS box transcription enhancer factor 2, polypeptide C is a protein that in humans is encoded by the MEF2C gene. (wikipedia.org)
Myocyte3
  • The small muscle-specific protein Csl modifies cell shape and promotes myocyte fusion in an insulin-like growth factor 1-dependent manner. (ox.ac.uk)
  • The activities of transcription factors nuclear factor of activated T cells (NFAT) and myocyte enhancer-binding factor (MEF)2, were also enhanced in an IGF-1 signaling-dependent manner. (ox.ac.uk)
  • Of particular importance has been the identification of two families of transcription factors: the myogenic regulatory factors (MRFs) and the myocyte enhancer factors (MEFs). (biologists.com)
Myogenesis1
  • Nonetheless, the enhanced myogenesis leads to the formation of an extensive network of contractile myofibers, and this enhanced myogenesis can be conferred upon myogenic cells from clonal populations, such as G8 or C2C12 cells, if they are co-cultured with the limb mesenchymal cells. (biologists.com)
MyoD3
  • Alter J, Bengal E. (2011) Stress-Induced C/EBP Homology Protein (CHOP) Represses MyoD Transcription to Delay Myoblast Differentiation . (technion.ac.il)
  • Ciechanover, A., Breitschopf, K., Abu Hatoum, O., and Bengal E. (1999) Degradation of MyoD by the ubiquitin pathway: regulation by specific DNA-binding and identification of a novel site for ubiquitination . (technion.ac.il)
  • Tamir, Y., and Bengal, E. (1998) p53 protein is activated during muscle differentiation and participates with MyoD in the transcription of muscle creatine kinase gene . (technion.ac.il)
Transcriptional regulation4
  • Thus, these studies identify the HRC enhancer as the first MEF2-dependent, CArG-independent transcriptional target in smooth muscle and represent the first analysis of the transcriptional regulation of an SR gene in vivo. (ox.ac.uk)
  • Kolpakova A*, Katz S*, Keren A, Rojtblat A, Bengal E . (2013) Transcriptional Regulation of Mesoderm Genes by MEF2D during Early Xenopus Development . (technion.ac.il)
  • The mechanisms that regulate myoblast cell proliferation versus differentiation involve extracellular signaling via growth factors, their cognate receptors, intracellular signal transduction cascades, and transcriptional regulation of genes associated with cell proliferation. (rosalindfranklin.edu)
  • We are interested in the transcriptional regulation of the FGFR1 gene because its expression is tightly linked to continued myoblast proliferation. (rosalindfranklin.edu)
Cardiac5
  • HRC is a direct transcriptional target of MEF2 during cardiac, skeletal, and arterial smooth muscle development in vivo. (ox.ac.uk)
  • We have isolated a murine cDNA encoding a 9-kD protein, Chisel (Csl), in a screen for transcriptional targets of the cardiac homeodomain factor Nkx2-5. (ox.ac.uk)
  • In this review, we focus on the role of cardiomyocyte-derived and cardiac fibroblast-derived microRNAs that are involved in the regulation of genes associated with cardiomyocyte and fibroblast function and in atherosclerosis-related cardiac ischemia. (archivesofmedicalscience.com)
  • We are also exploring the mechanisms that control myogenic cell proliferation versus differentiation in both skeletal and cardiac muscle development. (rosalindfranklin.edu)
  • Transcription factor GATA4-targeted compounds that have previously shown in vivo efficacy in cardiac injury models were tested for stage-specific activation of atrial and ventricular reporter genes in differentiating pluripotent stem cells using a dual reporter assay. (biomedcentral.com)
Regulatory5
  • The dynamic cytoskeletal localization of Csl and its dominant effects on cell shape and behavior and transcription factor activity suggest that Csl plays a role in the regulatory network through which muscle cells coordinate their structural and functional states during growth, adaptation, and repair. (ox.ac.uk)
  • Given the significance of myogenin regulation in adult muscle, the Akt/MSY3/myogenin regulatory circuit is a potential therapeutic target to counteract muscle degenerative disease. (biomedcentral.com)
  • Basic helix-loop-helix (bHLH) myogenic regulatory factors coordinate the correct function and organization of many skeletal muscle functions. (biomedcentral.com)
  • The aim of this research is to identify and characterize the molecular regulatory circuitry that establishes these myogenic cell lineages and which thereby establish fast versus fast/slow muscle fiber types, independent of innervation, and dependent of cell lineage commitment. (rosalindfranklin.edu)
  • Transcription Factor Binding Sites (TFBSs) are small nucleotide fragments (usually ≤ 30 bp) in the cis-regulatory/intergenic regions in DNA sequences. (biomedcentral.com)
Myosin2
  • Specifically,treatment of primary limb bud cultures with p38 inhibitors increases the expression of myogenic markers and causes a striking increase in formation of myotubes, which were detected using antibodies specific for myosin heavy chain. (biologists.com)
  • This signaling culminates in regulation of transcription factors governing expression of the slow myosin heavy chain 2 gene - indicative of the slow muscle fiber phenotype. (rosalindfranklin.edu)
MEF2C2
  • MEF2C is a transcription factor in the Mef2 family. (wikipedia.org)
  • Zetser, A., Gredinger, E., and Bengal, E. (1999) p38 mitogen-activated protein kinase promotes skeletal muscle differentiation: participation of the MEF2C transcription factor . (technion.ac.il)
C2C122
  • Skeletal muscle and myogenic C2C12 cells were used to study the effects of MSY3 phosphorylation in vivo and in vitro on its sub-cellular localization and activity, by blocking the IGF1/PI3K/Akt pathway, by Akt depletion and over-expression, and by mutating potential MSY3 phosphorylation sites. (biomedcentral.com)
  • In C2C12 myogenic cells, blocking the IGF1/PI3K/Akt pathway using LY294002 inhibitor reduced MSY3 phosphorylation levels resulting in its accumulation in the nuclei. (biomedcentral.com)
Protein1
  • The Y-box protein MSY3/Csda represses myogenin transcription in skeletal muscle by binding a highly conserved cis-acting DNA element located just upstream of the myogenin minimal promoter (myogHCE). (biomedcentral.com)
Embryonic2
  • More recently, we have initiated studies to investigate the molecular mechanisms that control the development of distinct myogenic cell lineages during embryonic mygenesis. (rosalindfranklin.edu)
  • Regulation of gene expression plays a central role in embryonic development. (biomedcentral.com)
Mechanisms2
Expression2
  • While Akt over-expression rescued myogenin expression in MSY3 overexpressing myogenic cells, ablation of the Akt substrate, (Ser126 located in the MSY3 cold shock domain) promoted MSY3 accumulation in the nucleus and abolished this rescue. (biomedcentral.com)
  • This study highlights a previously undescribed Akt-mediated signaling pathway involved in the repression of myogenin expression in myogenic cells and in mature muscle. (biomedcentral.com)
Muscle2
  • Research in our lab is based on avian model systems in which both intrinsic and extrinsic regulation of muscle fiber type is analyzed. (rosalindfranklin.edu)
  • Compounds that target tissue-specific transcription factors could overcome non-specific effects of small molecules and lead to the regeneration of heart muscle following myocardial infarction. (biomedcentral.com)
Coordinate1
  • To identify genes that depend upon, or are involved in, this coordinate regulation we screened for heterozygous chromosome 3 deficiencies that impair development of flies bearing reduced H3.2 and H3.3 gene copy number. (bvsalud.org)
Myoblasts2
  • Csl-expressing myoblasts differentiated and fused normally, although in the presence of insulin-like growth factor (IGF)-1 they showed dramatically enhanced fusion, leading to formation of large dysmorphogenic "myosacs. (ox.ac.uk)
  • Among these genes is the fibroblast growth factor receptor 1 (FGFR1) gene which is expressed in proliferating myoblasts and down-regulated during differentiation. (rosalindfranklin.edu)
Promoter1
  • We have previously shown that MSY3/Csda binds a highly conserved cis-acting element located upstream of the myogenin promoter (myogHCE) and regulates the postnatal down-regulation of myogenin [ 16 ]. (biomedcentral.com)
Differentiation1
  • Alter, J., Rozentzweig, D., and Bengal, E. (2008) Inhibition of myoblast differentiation by tumor necrosis factor alpha is mediated by c-Jun N-terminal kinase 1 and leukemia inhibitory factor . (technion.ac.il)
Cell1
Identification2
  • Identification of transcription factor binding sites (TFBSs) is a central problem in Bioinformatics on gene regulation. (biomedcentral.com)
  • Therefore, TFBSs are a critical component in gene regulation, and identification of TFBSs is a central problem for understanding gene regulation in molecular biology. (biomedcentral.com)
Basic1
  • This gene encodes a basic motif-leucine zipper transcription factor of the Maf subfamily. (en-academic.com)
Attenuated myogenic differentiation1
  • In human myoblasts, OIP5-AS1 levels increased robustly early in myogenesis, and its loss attenuated myogenic differentiation and potently reduced the levels of the myogenic transcription factor MEF2C. (nih.gov)
Genes7
  • Transcription of miR genes by RNA polymerase II form primary transcripts known as primary miR (pri-miR). (medscape.com)
  • 3. SFMBT1 functions with LSD1 to regulate expression of canonical histone genes and chromatin-related factors. (nih.gov)
  • 16. SH2B1 modulates chromatin state and MyoD occupancy to enhance expressions of myogenic genes. (nih.gov)
  • GO enrichment analysis for the host genes of DE circRNAs showed that biological process (BP) terms in the top 20 related to growth in F14vsF20 were found such as positive regulation of transcription involved in G1/S phase of mitotic cell cycle, multicellular organismal macromolecule metabolic process, and multicellular organismal metabolic process. (researchsquare.com)
  • Mitochondria have circular DNA (mitochondrial DNA, mtDNA), and mitochondrial biogenesis (mitobiogenesis) involves the replication, transcription, and translation of mtDNA-encoded genes, the interorganelle transport of phospholipids, and the import of nuclear-encoded proteins into mitochondria through the protein translocation machinery of the outer and inner membranes [ 14 ]. (biomedcentral.com)
  • Given that the majority of mitochondrial proteins are nuclear-encoded, the transcription and translation of nuclear and mitochondrial genes must be tightly coordinated to ensure the creation of new mitochondria [ 17 ]. (biomedcentral.com)
  • This view reflects the amazing conservation of genetic apparatus that has been discovered across the Bilateria, particularly of genes encoding transcription factors and signaling molecules. (silverchair.com)
Skeletal muscle5
  • We also find that like growth factor-1 (IGF-1) has a key role in the regulation of MG53 protein level is not upregulated in the muscle samples skeletal muscle size. (marysfamilymedicine.org)
  • suggesting that MG53 upregulation is not a causative factor In vivo injection of IGF-1 in skeletal muscle or skeletal muscle- for the development of insulin resistance. (marysfamilymedicine.org)
  • Exercise metabolism and the molecular regulation of skeletal muscle adaptation. (academicinfluence.com)
  • Tumor necrosis factor-alpha induces skeletal muscle insulin resistance in healthy human subjects via inhibition of Akt substrate 160 phosphorylation. (academicinfluence.com)
  • Expression of Mfn2, the Charcot-Marie-Tooth neuropathy type 2A gene, in human skeletal muscle: effects of type 2 diabetes, obesity, weight loss, and the regulatory role of tumor necrosis factor alpha and interleukin-6. (academicinfluence.com)
20021
  • 2002). The Drosophila zinc finger transcription factor CF2 is a myogenic marker downstream of MEF2 during muscle development. (geneticsmr.com)
Transcriptional3
  • 17. Association of COOH-terminal-binding protein (CtBP) and MEF2-interacting transcription repressor (MITR) contributes to transcriptional repression of the MEF2 transcription factor. (nih.gov)
  • 2004). Distributions of Z-DNA and nuclear factor I in human chromosome 22: a model for coupled transcriptional regulation. (geneticsmr.com)
  • The transcriptional factor CF2 is a mediator of EGF-R-activated dorsoventral patterning in Drosophila oogenesis. (geneticsmr.com)
Epigenetic1
  • 1. Proteomic and functional analyses reveal the role of chromatin reader SFMBT1 in regulating epigenetic silencing and the myogenic gene program. (nih.gov)
Gene encodes2
  • The SRY gene encodes the testis determining factor , which is also referred to as the SRY protein . (wikidoc.org)
  • This intronless gene encodes a transcription factor that is a member of the high mobility group (HMG)-box family of DNA-binding proteins. (wikidoc.org)
Chromatin3
Regulate1
  • Because its regulation in muscle has not been fully exploited, the aim of this project was to identify micro (mi)RNAs in muscle that regulate Twist-1. (biologists.com)
Molecular regulation1
  • We review the molecular regulation of both mitophagy and mitobiogenesis processes and give special attention to the crosstalk between them that fine tunes the balance of mitochondrial mass and quality. (biomedcentral.com)
Genome1
  • miRs that are derived from their own transcription units are located in intergenic regions of the genome. (medscape.com)
Muscle1
  • Muscle samples derived from human diabetic patients and mice with insulin resistance show normal expression of MG53, indicating that altered MG53 expression does not serve as a causative factor for the development of metabolic disorders. (marysfamilymedicine.org)
Activation1
  • Activation of the cellular senescence genetic program prompts a series of molecular changes, mostly affecting cell cycle, extracellular matrix (ECM), secretion of growth factors, and inflammatory mediators. (hindawi.com)
Mice1
  • We have previously reported the up-regulation of galectin-3, a β-galactoside-binding lectin, in the endothelium and sera of diabetic mice, implicating this molecule in diabetic vasculopathy and suggesting its potential as a biomarker of the disease. (biomedcentral.com)
Response1
  • In this review, we will first summarize the recent advances made in the discovery of molecular regulations of mitobiogenesis and mitophagy and then focus on the mechanism and signaling pathways involved in the simultaneous regulation of mitobiogenesis and mitophagy in the response of tissue or cultured cells to energy needs, stress, or pathophysiological conditions. (biomedcentral.com)