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Transcription FactorsMEF2 Transcription FactorsTranscription, GeneticDNA-Binding ProteinsPromoter Regions, GeneticSp1 Transcription FactorGene Expression RegulationMolecular Sequence DataBase SequenceTranscriptional ActivationBinding SitesTrans-ActivatorsMyogenic Regulatory FactorsBasic Helix-Loop-Helix Transcription FactorsRNA, MessengerProtein BindingNuclear ProteinsTranscription Factor AP-1Repressor ProteinsCell LineGene Expression Regulation, DevelopmentalForkhead Transcription FactorsHomeodomain ProteinsSignal TransductionAmino Acid SequenceDNABasic-Leucine Zipper Transcription FactorsMutationTranscription Factor AP-2Cell NucleusCell DifferentiationTransfectionKruppel-Like Transcription FactorsTranscription Factors, TFIIGenes, ReporterChromatin ImmunoprecipitationGATA4 Transcription FactorHeLa CellsCells, CulturedYY1 Transcription FactorSTAT3 Transcription FactorTranscription Factor TFIIDNFATC Transcription FactorsActivating Transcription Factor 3Reverse Transcriptase Polymerase Chain ReactionElectrophoretic Mobility Shift AssaySp3 Transcription FactorNF-kappa BEnhancer Elements, GeneticTranscription Initiation SiteZinc FingersPaired Box Transcription FactorsActivating Transcription Factor 2Transcription Factor TFIIBRegulatory Sequences, Nucleic AcidGene Expression ProfilingE2F1 Transcription FactorRecombinant Fusion ProteinsCloning, MolecularRNA Polymerase IIPlasmidsBasic Helix-Loop-Helix Leucine Zipper Transcription FactorsGene ExpressionGATA3 Transcription FactorGATA1 Transcription FactorGATA2 Transcription FactorGene Expression Regulation, FungalTCF Transcription FactorsGATA Transcription FactorsLuciferasesProtein Structure, TertiaryMicrophthalmia-Associated Transcription FactorMADS Domain ProteinsPhosphorylationSTAT1 Transcription FactorActivating Transcription FactorsCell Line, TumorHelix-Loop-Helix MotifsTranscription Factor RelAE2F Transcription FactorsChromatinOligonucleotide Array Sequence AnalysisSaccharomyces cerevisiaeModels, BiologicalSequence Homology, Amino AcidSaccharomyces cerevisiae ProteinsGene Expression Regulation, PlantMice, KnockoutCyclic AMP Response Element-Binding ProteinGATA6 Transcription FactorActivating Transcription Factor 4Transcription Factor 7-Like 1 ProteinActivating Transcription Factor 1Blotting, WesternProto-Oncogene ProteinsTranscription Factor TFIIIAProto-Oncogene Proteins c-junMice, TransgenicDown-RegulationDrosophila Proteins
TranscriptionalDifferentiationProteinsMEF2AGenesSkeletal muscleEnhancerGeneC2C12BHLHExpressionRegulationEmbryonicTissue-specificPathwayMechanismsRolesMuscleCellBinding sitesPlaysDomainComplexBasic helix-loop-helix transcripInhibits myogenicMyogenesisMyoDMyocyteMyogeninTightly regulatedExtracellularCardiacProteinMutationsFamilyGenomeDevelopmentCellsResults
Transcriptional5
  • MicroRNAs regulate gene expression at the post-transcriptional level by binding to 3′- or less often to 5′-untranslated regions of target messenger RNAs (mRNAs), which in consequence leads to inhibited translation and/or induces degradation of targeted mRNA [ 1 ]. (archivesofmedicalscience.com)
  • Mechanistically, ICN1 upregulated expression of SNAIL1, a transcriptional repressor, to increase TPC number in human ERMS and to block muscle differentiation through suppressing MEF2C, a myogenic differentiation transcription factor. (duke.edu)
  • Tamir, Y., and Bengal E . (2000) Phosphoinositide 3-kinase induces the transcriptional activity of MEF2 proteins during muscle differentiation . (technion.ac.il)
  • The mechanisms that regulate myoblast cell proliferation versus differentiation involve extracellular signaling via growth factors, their cognate receptors, intracellular signal transduction cascades, and transcriptional regulation of genes associated with cell proliferation. (rosalindfranklin.edu)
  • Our published findings demonstrate that the FGFR1 gene is positively regulated by the Sp1 transcriptional regulator and negatively regulated by the transcription factor E2F4. (rosalindfranklin.edu)
Differentiation11
  • The process of differentiation from mesodermal precursor cells to myoblasts has led to the discovery of a variety of tissue-specific factors that regulate muscle gene expression. (wikipedia.org)
  • This review concentrates on the research progress of the non-canonical Hippo pathway in regulating innate immune cell anti-infection responses, maintaining redox homeostasis, responding to microenvironmental stiffness, and T-cell differentiation. (bvsalud.org)
  • Alter J, Bengal E. (2011) Stress-Induced C/EBP Homology Protein (CHOP) Represses MyoD Transcription to Delay Myoblast Differentiation . (technion.ac.il)
  • Alter, J., Rozentzweig, D., and Bengal, E. (2008) Inhibition of myoblast differentiation by tumor necrosis factor alpha is mediated by c-Jun N-terminal kinase 1 and leukemia inhibitory factor . (technion.ac.il)
  • Zetser, A., Gredinger, E., and Bengal, E. (1999) p38 mitogen-activated protein kinase promotes skeletal muscle differentiation: participation of the MEF2C transcription factor . (technion.ac.il)
  • Tamir, Y., and Bengal, E. (1998) p53 protein is activated during muscle differentiation and participates with MyoD in the transcription of muscle creatine kinase gene . (technion.ac.il)
  • The embryonic AMPs are at the origin of all adult fly muscles and, as we demonstrate here, they express repressors of myogenic differentiation and targets of the Notch pathway known to be involved in muscle cell stemness. (biologists.com)
  • Much of our research focuses on the cellular and molecular mechanisms that regulate the development and differentiation of skeletal muscle fibers and their respective phenotypes. (rosalindfranklin.edu)
  • We are also exploring the mechanisms that control myogenic cell proliferation versus differentiation in both skeletal and cardiac muscle development. (rosalindfranklin.edu)
  • Among these genes is the fibroblast growth factor receptor 1 (FGFR1) gene which is expressed in proliferating myoblasts and down-regulated during differentiation. (rosalindfranklin.edu)
  • These studies provide insight into mechanisms that regulate not only muscle cell proliferation and differentiation, but regulation of cell proliferation in general. (rosalindfranklin.edu)
Proteins7
  • The myogenic basic helix-loop-helix proteins, including myoD (MIM 159970), myogenin (MIM 159980), MYF5 (MIM 159990), and MRF4 (MIM 159991) are 1 class of identified factors. (wikipedia.org)
  • A second family of DNA binding regulatory proteins is the myocyte-specific enhancer factor-2 (MEF2) family. (wikipedia.org)
  • Each of these proteins binds to the MEF2 target DNA sequence present in the regulatory regions of many, if not all, muscle-specific genes. (wikipedia.org)
  • It is estimated that microRNAs regulate production of approximately 60% of all human proteins and enzymes that are responsible for major physiological processes. (archivesofmedicalscience.com)
  • MSY3 (MSY4, YB-2, CSDA, dbpA, ZONAB) is a member of the cold shock domain (CSD) family of proteins also known as Y-box proteins, which are evolutionarily conserved proteins that function as transcription factors and regulators of RNA metabolism and protein synthesis. (biomedcentral.com)
  • A hallmark of events observed during segmentation was the induction of multiple transcription factors, including a large group of homeobox proteins in pace with decay of a large fraction of maternal transcripts. (biomedcentral.com)
  • Regulatory proteins, namely the Transcription Factors (TFs), bind in a sequence-specific manner to TFBSs to activate or suppress gene transcription (gene expression). (biomedcentral.com)
MEF2A2
  • Myocyte-specific enhancer factor 2A is a protein that in humans is encoded by the MEF2A gene. (wikipedia.org)
  • MEF2A is a transcription factor in the Mef2 family. (wikipedia.org)
Genes2
  • The MEF2 genes are members of the MADS gene family (named for the yeast mating type-specific transcription factor MCM1, the plant homeotic genes 'agamous' and 'deficiens' and the human serum response factor SRF (MIM 600589)), a family that also includes several homeotic genes and other transcription factors, all of which share a conserved DNA-binding domain. (wikipedia.org)
  • Transcription factor GATA4-targeted compounds that have previously shown in vivo efficacy in cardiac injury models were tested for stage-specific activation of atrial and ventricular reporter genes in differentiating pluripotent stem cells using a dual reporter assay. (biomedcentral.com)
Skeletal muscle3
  • The Y-box protein MSY3/Csda represses myogenin transcription in skeletal muscle by binding a highly conserved cis-acting DNA element located just upstream of the myogenin minimal promoter (myogHCE). (biomedcentral.com)
  • Skeletal muscle and myogenic C2C12 cells were used to study the effects of MSY3 phosphorylation in vivo and in vitro on its sub-cellular localization and activity, by blocking the IGF1/PI3K/Akt pathway, by Akt depletion and over-expression, and by mutating potential MSY3 phosphorylation sites. (biomedcentral.com)
  • Basic helix-loop-helix (bHLH) myogenic regulatory factors coordinate the correct function and organization of many skeletal muscle functions. (biomedcentral.com)
Enhancer1
Gene4
  • Published results indicate that protein kinase C (PKC) activity in conjunction with innervation-induced activation of cell signaling mediated by the muscarinic acetylcholine receptor, Gaq, and the 1,4,5 inositol triphosphate receptor 1 (IP3R1) differentially regulate fast versus slow muscle fiber type gene expression in innervated muscle fibers. (rosalindfranklin.edu)
  • This signaling culminates in regulation of transcription factors governing expression of the slow myosin heavy chain 2 gene - indicative of the slow muscle fiber phenotype. (rosalindfranklin.edu)
  • Identification of transcription factor binding sites (TFBSs) is a central problem in Bioinformatics on gene regulation. (biomedcentral.com)
  • This gene encodes a basic motif-leucine zipper transcription factor of the Maf subfamily. (en-academic.com)
C2C122
  • Nonetheless, the enhanced myogenesis leads to the formation of an extensive network of contractile myofibers, and this enhanced myogenesis can be conferred upon myogenic cells from clonal populations, such as G8 or C2C12 cells, if they are co-cultured with the limb mesenchymal cells. (biologists.com)
  • In C2C12 myogenic cells, blocking the IGF1/PI3K/Akt pathway using LY294002 inhibitor reduced MSY3 phosphorylation levels resulting in its accumulation in the nuclei. (biomedcentral.com)
BHLH2
  • The bHLH transcription factor TWIST1 plays a key role in the embryonic development and in tumorigenesis. (biomedcentral.com)
  • TWIST1 is a transcription factor belonging to the basic helix-loop-helix (bHLH) superfamily. (biomedcentral.com)
Expression3
  • Specifically,treatment of primary limb bud cultures with p38 inhibitors increases the expression of myogenic markers and causes a striking increase in formation of myotubes, which were detected using antibodies specific for myosin heavy chain. (biologists.com)
  • While Akt over-expression rescued myogenin expression in MSY3 overexpressing myogenic cells, ablation of the Akt substrate, (Ser126 located in the MSY3 cold shock domain) promoted MSY3 accumulation in the nucleus and abolished this rescue. (biomedcentral.com)
  • This study highlights a previously undescribed Akt-mediated signaling pathway involved in the repression of myogenin expression in myogenic cells and in mature muscle. (biomedcentral.com)
Regulation2
  • We have previously shown that MSY3/Csda binds a highly conserved cis-acting element located upstream of the myogenin promoter (myogHCE) and regulates the postnatal down-regulation of myogenin [ 16 ]. (biomedcentral.com)
  • This indicated the involvement of epigenetic modulators in the regulation of GATA-dependent transcription. (biomedcentral.com)
Embryonic1
  • More recently, we have initiated studies to investigate the molecular mechanisms that control the development of distinct myogenic cell lineages during embryonic mygenesis. (rosalindfranklin.edu)
Tissue-specific1
  • Compounds that target tissue-specific transcription factors could overcome non-specific effects of small molecules and lead to the regeneration of heart muscle following myocardial infarction. (biomedcentral.com)
Pathway1
  • Scholars@Duke publication: The NOTCH1/SNAIL1/MEF2C Pathway Regulates Growth and Self-Renewal in Embryonal Rhabdomyosarcoma. (duke.edu)
Mechanisms1
  • However, mechanisms regulating TPC self-renewal are largely unknown, especially in embryonal rhabdomyosarcoma (ERMS)-a common pediatric cancer of muscle. (duke.edu)
Roles2
  • In addition to regulating physiological nitric oxide signaling and responses of cells to stress, studies in mice lacking thrombospondin-1 or its receptors have revealed important roles for thrombospondin-1 in regulating immune responses in infectious and autoimmune diseases and antitumor immunity. (bvsalud.org)
  • ICN1 also had conserved roles in regulating human ERMS self-renewal and growth. (duke.edu)
Muscle1
  • The aim of this research is to identify and characterize the molecular regulatory circuitry that establishes these myogenic cell lineages and which thereby establish fast versus fast/slow muscle fiber types, independent of innervation, and dependent of cell lineage commitment. (rosalindfranklin.edu)
Cell1
Binding sites1
  • Transcription Factor Binding Sites (TFBSs) are small nucleotide fragments (usually ≤ 30 bp) in the cis-regulatory/intergenic regions in DNA sequences. (biomedcentral.com)
Plays1
  • This study provides evidence that hypothalamic TRPV4 plays a significant role in regulating homeostasis. (bvsalud.org)
Domain1
  • The FLNC subunit is dimerized through R24 and calpain cleaves off the dimerization domain to regulate mobility of the FLNC subunit. (omicsdi.org)
Complex1
  • After transcription, Pri-miRNA is cleaved by the enzymatic complex DROSHA into a micro-RNA precursor (pre-miRNA). (archivesofmedicalscience.com)
Basic helix-loop-helix transcrip1
  • 14. Target gene selectivity of the myogenic basic helix-loop-helix transcription factor myogenin in embryonic muscle. (nih.gov)
Inhibits myogenic1
  • The MSC:E-protein heterodimer binds to E-boxes and inhibits myogenic reporters and MyoD-mediated myogenesis [ 10 ]. (biomedcentral.com)
Myogenesis4
  • 12. Deficiency in rhabdomyosarcomas of a factor required for MyoD activity and myogenesis. (nih.gov)
  • As one of many transcription factors that impede myogenesis, its binding on a genome-wide scale relative to the widespread binding of the myogenic factor MyoD is unknown. (biomedcentral.com)
  • A family of muscle-specific transcription factors which bind to DNA in control regions and thus regulate myogenesis. (nih.gov)
  • Adult skeletal muscle mass is IRS-1 ubiquitination with the help of the E2 enzyme UBE2H plastically regulated by recruiting satellite cells to preexisting during skeletal myogenesis by examining MG53-disrupted muscle fibres under hypertrophic conditions such as resistance skeletal muscle cells and tissues. (marysfamilymedicine.org)
MyoD6
  • The myogenic basic helix-loop-helix proteins, including myoD (MIM 159970), myogenin (MIM 159980), MYF5 (MIM 159990), and MRF4 (MIM 159991) are 1 class of identified factors. (wikipedia.org)
  • 11. Determinants of myogenic specificity within MyoD are required for noncanonical E box binding. (nih.gov)
  • 13. Mutation of Thr115 in MyoD positively regulates function in murine fibroblasts and human rhabdomyosarcoma cells. (nih.gov)
  • 15. Expression of myogenic regulating factors, Myogenin and MyoD, in two canine botryoid rhabdomyosarcomas. (nih.gov)
  • The myogenic inhibitor MSC binds throughout the genome of rhabdomyosarcoma cells, in a pattern highly similar to that of MyoD, suggesting a broad role in buffering the activity of MyoD in development and rhabdomyosarcomas. (biomedcentral.com)
  • The myogenic activity of MyoD can be inhibited by a variety of transcription factors, including other members of the bHLH protein family [ 4 ]. (biomedcentral.com)
Myocyte3
  • Myocyte-specific enhancer factor 2A is a protein that in humans is encoded by the MEF2A gene. (wikipedia.org)
  • A second family of DNA binding regulatory proteins is the myocyte-specific enhancer factor-2 (MEF2) family. (wikipedia.org)
  • The transcription factors in the myocyte enhancer factor 2 (MEF2) family play important roles in cell survival by regulating nuclear gene expression. (nih.gov)
Myogenin1
Tightly regulated1
  • Mitochondrial mass and quality are tightly regulated by two essential and opposing mechanisms, mitochondrial biogenesis (mitobiogenesis) and mitophagy, in response to cellular energy needs and other cellular and environmental cues. (biomedcentral.com)
Extracellular1
  • Activation of the cellular senescence genetic program prompts a series of molecular changes, mostly affecting cell cycle, extracellular matrix (ECM), secretion of growth factors, and inflammatory mediators. (hindawi.com)
Cardiac1
  • This is at least partly due to a progressive impairment of the cellular processes regulating cardiac and vascular homeostasis, finally leading to the development of cardiovascular pathologies. (hindawi.com)
Protein1
  • 16. Monoclonal antibodies to the myogenic regulatory protein MyoD1: epitope mapping and diagnostic utility. (nih.gov)
Mutations1
Family1
  • MEF2A is a transcription factor in the Mef2 family. (wikipedia.org)
Genome1
  • miRs that are derived from their own transcription units are located in intergenic regions of the genome. (medscape.com)
Development1
  • 2002). The Drosophila zinc finger transcription factor CF2 is a myogenic marker downstream of MEF2 during muscle development. (geneticsmr.com)
Cells1
  • At the molecular level, in fact, a gradual weakening of the cellular processes regulating cardiovascular homeostasis occurs in aging cells. (hindawi.com)
Results2
  • Ingenuity® Pathway Analysis was used to identify pathways up-regulated by HFD in the KO, such as the coagulation cascade, and measurements of blood clotting activity were performed to confirm these results. (biomedcentral.com)
  • Our results point to Mef2 and miR-133 as central upstream regulators of Prdm16 and hence of brown adipogenesis in response to cold exposure in BAT and SAT. (unige.ch)