Myogenic precursor cells. Medical search. Frequent questions

Those growth factors are known to improve the proliferative activity of myogenic precursor cells. (cellsurgicalnetwork.com)

Proliferating, BrdU-positive cells in muscle sections were detected with immunohistochemistry indicating different cell types and decreasing proliferation with age. (nature.com)
Cell culture experiments indicated that Gln could promote cell proliferation in a dose dependent manner, but expression of myogenesis regulatory genes was not altered. (nature.com)
Overall, Gln supplementation stimulated cell proliferation in muscle tissue and in vitro in myogenic cell culture, whereas muscle growth regulatory genes were barely altered. (nature.com)
Our team has demonstrated some important differences between man and mouse, in the regulation of the proliferation of myogenic precursors. (institut-myologie.org)
Hepatocyte growth factor (HGF) is produced by stromal and mesenchymal cells, and it stimulates epithelial cell proliferation, motility, morphogenesis and angiogenesis in various organs via tyrosine phosphorylation of its cognate receptor, Met. (spandidos-publications.com)
The polycomb-group protein EZH2 is a Lys27 histone H3 methyltransferase that regulates the balance between cell proliferation and differentiation by epigenetically silencing muscle-specific genes. (figshare.com)
Plays a role in myoblast differentiation and also in the down-regulation of cyclin D1 in response to hypoxia in adrenal cells suggesting MAPK12 may inhibit cell proliferation while promoting differentiation. (idrblab.net)
Macrophages expressing pro-inflammatory markers are abundant in regenerating regions damaging for Myog (a transcription aspect expressed only in differentiated myogenic cells) suggesting various associations depending on proliferation or differentiation of myogenic precursors [78,79]. (rockinhibitor.com)
IFN- IFN-, a pro-inflammatory cytokine, favors myoblast proliferation, prevents fibrotic events in SkMR, and is expressed by proliferating myoblasts although not by differentiated cells. (rockinhibitor.com)
decreased cell proliferation, cell cycle arrest, and induction of apoptosis. (123dok.net)
It was suggested that HOXD9 contributes to both cell proliferation and/or cell survival. (123dok.net)
HOXD9 siRNA treatment of SK-MG-1 SP cells resulted in reduced cell proliferation. (123dok.net)
Conclusions: Our results suggest that HOXD9 may be a novel marker of GCSCs and cell proliferation and/or survival factor in gliomas and glioma cancer stem-like cells, and a potential therapeutic target. (123dok.net)
Homeobox proteins are master regulators of develop-ment and control many cellular processes, including proliferation, apoptosis, cell shape, and cell migration. (123dok.net)
However, diprotin A, a positive modulator of CXCR4-SDF-1 binding, significantly enhanced engraftment and stimulated sustained proliferation of donor cells in vivo . (biomedcentral.com)
Diprotin A stimulation of CXCR4, however, significantly increased the number of canine dystrophin-positive muscle fibers and canine-derived satellite cells by enhancing donor cell proliferation. (biomedcentral.com)
SFK inhibition also induced muscle differentiation in RMS cells by affecting the NOTCH3 receptor-p38 mitogen-activated protein kinase (MAPK) axis, which regulates the balance between proliferation and differentiation. (oncotarget.com)

Specifically, the muscle progenitor cells, called satellite cells, are activated in the apparent absence of injury. (umn.edu)
Ultimately, we hope to transplant our EOM-derived progenitor cells into injured limb muscle of mdx mice in order to determine the regenerative capabilities in vivo . (umn.edu)
Mesodermal iPSC-derived progenitor cells functionally regenerate cardiac and skeletal muscle. (medecinesciences.org)
Myogenic progenitor cells from primary, iPSC or immortalized sources are resuspended in an ECM-derived hydrogel mixture, and are seeded into each culture chamber. (enuvio.com)
Moreover, one more of our recently published papers has identified new ways of making muscle progenitor cells to fuse more robustly into muscle fibers, hence enabling deliberate control of muscle tissue formation. (ca.gov)
We are at the latest stages in our work on the design of novel biomimetic stem cell niches, which based on our current results make easy to expand in culture progenitor cells (e.g. derived from paints) akin to muscle stem cells and enhancing the efficiency of cell transplantation to such an extent that progressive muscle loss in genetic myopathies is predicted to be averted. (ca.gov)
Her Progenitor cell research is multidisciplinary, incorporating elements of Myocyte, Myogenesis and Transcription factor. (research.com)
Progenitor cell, Cell growth, BMPR2 and Signal transduction is closely connected to Skeletal muscle in her research, which is encompassed under the umbrella topic of Stem cell. (research.com)
Finally, we cultured human glioma cancer stem cells (GCSCs) from patient specimens found with high expression of HOXD9 in GCSCs compared with normal astrocyte cells and neural stem/progenitor cells (NSPCs). (123dok.net)
Embryonic myogenic progenitor cells express CXCR4, G-protein coupled cell surface receptor, and migrate towards regions of SDF-1 expression during limb muscle development, suggesting that CXCR4/SDF-1 plays a role in muscle cell homing. (biomedcentral.com)

We had previously found that iSN04 promoted myogenic precursor cells (myoblasts) to differentiate into skeletal muscle cells and had hypothesized that the aptamer also enhanced differentiation of pluripotent stem cells. (shinshu-u.ac.jp)
Homeostasis of skeletal muscle tissue is maintained by myogenic precursor cells called myoblasts. (t-takaya.net)
After several rounds of cell divisions, myoblasts differentiate into contractile myocytes in order to regenerate muscle myofibers. (t-takaya.net)
In this talk, we introduce the effects of iSN04 on the myoblasts exposed to cancer secretion, the myoblasts from diabetic patients, and rhabdomyosarcoma cells from children. (t-takaya.net)
Previously, we reported that myoblasts (MBs) from slow-type enriched soleus (SOL) had a high potential to self-renew compared with cells derived from fast-type enriched tibialis anterior (TA). (bvsalud.org)
We also demonstrate that the transcriptional regulator PRDM16 (PRD1-BF1-RIZ1 homologous domain containing 16) controls a bidirectional cell fate switch between skeletal myoblasts and brown fat cells. (bioseek.eu)
Conversely, ectopic expression of PRDM16 in myoblasts induces their differentiation into brown fat cells. (bioseek.eu)
In addition, this proliferating impact is exerted on satellite cells after in vivo TNF- intraperitoneal injection [82], while Myog is lowered confirming differentiation inhibition of this cytokine on myoblasts [85]. (rockinhibitor.com)

Dystrophin expression in muscles of Duchenne muscular dystrophy patients after high-density injections of normal myogenic cells. (medecinesciences.org)
This review makes a critical examination of such background evidence, and also of more recent data directly addressing the concept of therapy by means of grafts of normal myogenic cells. (nih.gov)

It is shown that, after initial formation of the primary myotome, at least 15 muscle precursor cells (mpcs) seed each somite, where they proliferate but contribute little to muscle growth prior to hatching. (ox.ac.uk)

The main interest of Dr. Darabi's lab is to use pluripotent stem cells for skeletal muscle regeneration in muscular dystrophies and muscle injuries. (uh.edu)
During the last several years, his lab has developed novel methods for multi-lineage differentiation of pluripotent stem cells for cell therapy in mice models of muscular dystrophies and injuries. (uh.edu)
Derivation of Skeletal Myogenic Precursors from Human Pluripotent Stem Cells Using Conditional Expression of PAX7. (uh.edu)
Generating specific cell lineages from induced pluripotent stem cells and embryonic stem cells is the holy grail of regenerative medicine. (shinshu-u.ac.jp)
By demonstrating the generation of cardiomyocytes from murine pluripotent stem cells, their concept shows promise as a regenerative therapy. (shinshu-u.ac.jp)
An anti-nucleolin DNA aptamer, iSN04, regulates cardiomyogenesis of murine pluripotent stem cells. (shinshu-u.ac.jp)
Self-renewal and pluripotency-the capacity to form any cell lineage-are inherent characteristics of induced pluripotent stem cells (iPSCs). (shinshu-u.ac.jp)
However, when these pluripotent stem cells were treated after experiencing differentiating conditions for five days, specific marker genes were upregulated, and the cells committed to forming beating cardiomyocytes. (shinshu-u.ac.jp)
Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro. (medecinesciences.org)
Intriguingly, iSN04 is able to regulate the fate of pluripotent stem cells, which will be useful for regenerative medicine. (t-takaya.net)

To address questions of stem cell diversity during skeletal myogenesis, a Brainbow-like genetic cell lineage tracing method, dubbed Musclebow2, was derived by enhancer trapping in zebrafish. (ox.ac.uk)
Consensus has been reached that a specialized self-renewing stem cell termed the satellite cell plays the major role during the process of regeneration in amniotes. (monash.edu)
An intriguing aspect of this debate is the fact that a number of amphibia and many fish species have been shown to exhibit epimorphic regenerative processes in specific tissues, whereby regeneration occurs by the dedifferentiation of the damaged tissue itself, and does not deploy specialized stem cell populations analogous to satellite cells. (monash.edu)
These include generation of knock-in reporter stem cell lines to study myogenic differentiation, CRISPR/Cas9 gene targeting methods to correct mutated genes in muscular dystrophies, and using bioscaffolds, seeded with stem cells, to treat skeletal muscle injuries. (uh.edu)
Wu J, Matthias N, Lo J, Ortiz-Vitali JL, Shieh AW, Wang SH, Darabi R . A Myogenic Double-Reporter Human Pluripotent Stem Cell Line Allows Prospective Isolation of Skeletal Muscle Progenitors. (uh.edu)
Stem Cell Res. (uh.edu)
Wu J, Hunt SD, Matthias N, Servián-Morilla E, Lo J, Jafar-Nejad H, Paradas C, Darabi R . Generation of an induced pluripotent stem cell line (CSCRMi001-A) from a patient with a new type of limb-girdle muscular dystrophy (LGMD) due to a missense mutation in POGLUT1 (Rumi). (uh.edu)
Stem Cell Research. (uh.edu)
Matthias N, Hunt SD, Wu J, Darabi R . Skeletal muscle perfusion and stem cell delivery in muscle disorders using intra-femoral artery canulation in mice. (uh.edu)
Skoglund G, Lainé J, Darabi R , Fournier E, Perlingeiro R, Tabti N. Physiological and ultrastructural features of human induced pluripotent and embryonic stem cell-derived skeletal myocytes in vitro. (uh.edu)
Assessment of the myogenic stem cell compartment following transplantation of pax3/pax7-induced embryonic stem cell-derived progenitors. (uh.edu)
Aptamers are single-stranded pieces of DNA that bind to target proteins and are able to modulate signaling cascades during cell differentiation when a stem cell commits to a specific functional role or phenotype. (shinshu-u.ac.jp)
iSN04 treatment under differentiating conditions inhibited stem cell commitment to the cardiac lineage. (shinshu-u.ac.jp)
Stem cell function, self-renewal, and behavioral heterogeneity of cells from the adult muscle satellite cell niche. (medecinesciences.org)
Her primary areas of study are Cell biology, Neuroscience, Stem cell, Skeletal muscle and Phenotype. (research.com)
The concepts of her Stem cell study are interwoven with issues in Myogenesis, Human embryogenesis and Induced pluripotent stem cell. (research.com)
Her research in Schwann cell, HES1, Muscle stem cell, Axon and MAPK/ERK pathway are components of Cell biology. (research.com)
Stem cell antigen-1 (Sca-1) is a member of the Ly-6 multigene family encoding highly homologous, glycosyl-phosphatidylinositol-anchored membrane proteins. (plos.org)
Therefore, these results establish the efficacy of diprotin A in stimulating muscle cell engraftment, and highlight the pre-clinical utility of a xenotransplantation model in assessing the relative efficacy of muscle stem cell populations. (biomedcentral.com)
We used a clinically acceptable regimen of hematopoietic stem cell transplantation to establish mixed donor/host blood cell chimerism and immune tolerance in a canine model of Duchenne muscular dystrophy ( cxmd ) [ 9 ]. (biomedcentral.com)

Abstract Background Rhabdomyosarcoma (RMS) is a pediatric soft tissue sarcoma arising from myogenic precursors that have lost their capability to differentiate into skeletal muscle. (figshare.com)

Specifically,treatment of primary limb bud cultures with p38 inhibitors increases the expression of myogenic markers and causes a striking increase in formation of myotubes, which were detected using antibodies specific for myosin heavy chain. (biologists.com)
Essential role for the c-met receptor in the migration of myogenic precursor cells into the limb bud. (research.com)

We conclude that in the vertebrates so far examined, epimorphosis does not appear to be deployed during muscle regeneration, and that analogous cells expressing similar marker genes to satellite cells appear to be deployed during the regenerative process. (monash.edu)
More precisely we are working on RNA metabolism , muscle regeneration , muscle stem cells , and fibrosis , with the final aim of developing innovative therapeutic approaches. (institut-myologie.org)
Conditional Met KO mice were generated using Cre‑loxP methodology and characterization of these mice indicated that the HGF‑Met signaling pathway is essential in regeneration, protection, and homeostasis in various tissue types and cells. (spandidos-publications.com)
Direct isolation of satellite cells for skeletal muscle regeneration. (medecinesciences.org)
Alameddine HS, Dehaupas M, Fardeau M. Regeneration of skeletal muscle fibers from autologous satellite cells multiplied in vitro. (medecinesciences.org)
Identification of a novel population of muscle stem cells in mice: potential for muscle regeneration. (medecinesciences.org)
Muscle regeneration declines with aging and myopathies, and reprogramming of differentiated muscle cells to their progenitors can serve as a robust source of therapeutic cells. (ca.gov)
The reprogrammed muscle precursor cells contributed to muscle regeneration in vitro and in vivo and were unequivocally distinguished because of the lineage marking method. (ca.gov)
MAPK12-signaling is also positively regulating the expansion of transient amplifying myogenic precursor cells during muscle growth and regeneration. (idrblab.net)
In 2009 beginning of 2010 we have focused on investigating what factors human embryonic stem cells (hESCs) may produce that enhance regeneration and if those factors have any effects by themselves on regeneration. (ca.gov)
Finally, our data suggest that muscle stem cells either do not accumulate DNA damage with age or can efficiently repair such damage, when activated for tissue regeneration. (ca.gov)
For postnatal development and regeneration of skeletal muscle mass satellite television cells Mirtazapine a self-renewing pool of muscle mass stem cells give rise to child myogenic precursor cells that contribute to the formation of fresh muscle mass fibers. (mdm-inhibitors.com)

Human ES and iPS-Derived Myogenic Progenitors Restore Dystrophin and Improve Contractility upon Transplantation in Dystrophic Mice. (uh.edu)
These cells are also present in the EOM of mdx mice and mdx/utrophin -/- mice, models for DMD. (umn.edu)
In murine-to-murine transplantation experiments, CXCR4 expression marks a population of adult murine satellite cells with robust engraftment potential in mdx mice, and CXCR4-positive murine muscle-derived SP cells home more effectively to dystrophic muscle after intra-arterial delivery in mdx 5cv mice. (biomedcentral.com)
Indeed, CXCR4-positive muscle-derived side population (SP) cells home more effectively to dystrophic muscle after intra-arterial delivery in mdx 5cv mice [ 10 ]. (biomedcentral.com)

iSN04, the most effective myoDN, is spontaneously incorporated into cytoplasm and drive myogenic gene expression program. (t-takaya.net)
Global gene expression analysis revealed that the myogenic potential of MBs was independent of the muscle fiber type they reside in but dependent on the region of muscles they are derived from. (bvsalud.org)

The poor performance of stem cells in an aged organ, such as skeletal muscle, is caused by the changes in regulatory pathways such as Notch, MAPK and TGF‐β, where old differentiated tissues and blood circulation inhibit the regenerative performance of organ stem cells. (ca.gov)

The team, led by Associate Professor Tomohide Takaya from the Department of Agricultural and Life Sciences at Shinshu University, recently discovered that an anti-nucleolin aptamer, myogenetic oligodeoxynucleotide iSN04, induced myocardial differentiation in embryonic stem cells (ESCs). (shinshu-u.ac.jp)
Electrical stimulation of human embryonic stem cells: Cardiac differentiation and the generation of reactive oxygen species. (unipd.it)
We have also deciphered some of the fundamental properties of embryonic stem cells, which would enable deliberate control of their self-renewal and tissue specific differentiation and the manuscript describing these findings has been submitted to Cell. (ca.gov)

Wu J, Hunt SD, Xue H, Liu Y, Darabi R . Generation and Characterization of a MYF5 Reporter Human iPS Cell Line Using CRISPR/Cas9 Mediated Homologous Recombination. (uh.edu)
Here we show by in vivo fate mapping that brown, but not white, fat cells arise from precursors that express Myf5, a gene previously thought to be expressed only in the myogenic lineage. (bioseek.eu)
2009). We produced rodents in AZD8330 manufacture which was conditionally ablated in SCs (muscle mass knockout, mouse, where cells that communicate or possess ever indicated Myf5-Cre are YFP+, while cells that possess by no means indicated Myf5-Cre are YFP? (cancer-ecosystem.com)
A even more unsuspecting and plastic material chromatin environment, such as that of Sera cells or non-committed Pax7+/Myf5? (cancer-ecosystem.com)

In vivo , the identification of macrophage phenotype is complicated since macrophages are obviously exposed to a microenvironment that is more complex respect to cell culture conditions, and they display characteristics that do not conform to the in vitro defined phenotypic categories. (hindawi.com)
We have chosen to work on human precursors, either in vitro or in vivo, in xenograft models that we have developed in successive European networks. (institut-myologie.org)
I will perform the following experiments: 1) examine the phenotype and differentiation potential of these cells, 2) determine the mechanism for their resiliency to injury, and 3) examine their ability to repair or regenerate muscle in vivo . (umn.edu)
Defining the heterogeneity of skeletal muscle-derived side and main population cells isolated immediately ex vivo. (umn.edu)
Nicotinamide mononucleotide treatment, which restores the intracellular NAD+ level, could decrease p53 acetylation and increase myogenic cell self-renewal ability in vivo. (bvsalud.org)
Therefore, it appears that fetal myogenic precursor cells are present in embryonic cultures, but only differentiate in vivo. (spacecorphome.com)

No evidence of cell-lineage-based clonal fate diversity was obtained. (ox.ac.uk)
Ultimately, terminal cell differentiation commits to the cardiomyocyte lineage. (shinshu-u.ac.jp)
I have found a CD34+ group of cells in EOM that is negative for other lineage-specific markers. (umn.edu)
These studies support the existence of myoblast lineages and suggest that, although environmental cues can modulate fibre type expression, the intrinsic program of the myogenic lineage determines the extent of modulation that can occur. (spacecorphome.com)

Although functional organ stem cells persist in the old, tissue damage invariably overwhelms tissue repair, ultimately causing the demise of an organism. (ca.gov)
Our work is at the stage of understanding the molecular mechanisms by which the aging of the regenerative potential of organ stem cells can be reversed by particular human embryonic factors that are capable of neutralizing the affects of aged niches on tissue regenerative capacity. (ca.gov)
Thus, the use of hESC-produced pro-regenerative factors for boosting the regenerative capacity of organ stem cells is likely to yield healthy, young tissue. (ca.gov)
Renal parenchymal dysplasia is common and may be related to maldevelopment of the metanephric blastema (renal precursor tissue) in an environment of high intraluminal pressure. (medscape.com)

Clonal behaviour of myogenic precursor cells throughout the vertebrate lifespan. (ox.ac.uk)
This lack of information on the role of analogous cells to the satellite cell in other vertebrate systems is even more surprising given the fact that that satellite cells were first designated in frogs. (monash.edu)
However, the functional definition of these cells as self-renewing muscle stem cells remains a final hurdle to the definition of the satellite cell as a generic vertebrate cell type. (monash.edu)
The neural crest (NC) is comprised of specialized cells that arise from the lateral border of the neural plate and migrate into specific locations during vertebrate embryonic development. (jneurosci.org)

Alternatively activated M2 macrophages comprise cells exposed to low concentrations of M-CSF in the presence of IL-4, IL-13, or IL-10. (hindawi.com)
Furthermore, the stage with the muscle healing process influences the effects of macrophages on myogenic precursors. (rockinhibitor.com)
6. Cytokines and Muscle Healing Cytokines are also involved Ziritaxestat MedChemExpress within the complicated crosstalk among myogenic precursors and macrophages, as described below and summarized in Tables 4 and five, and Figure five).Int. J. Mol. (rockinhibitor.com)

Our hypothesis is that the EOM are spared from the degenerative effects of Duchenne muscular dystrophy (DMD) due to the presence of higher numbers of a unique precursor cell capable of maintaining a normal phenotype under conditions that normally result in atrophy. (umn.edu)
Sparing of extraocular muscle in aging and muscular dystrophies: a myogenic precursor cell hypothesis.Exp Cell Res. (umn.edu)
Systemic delivery of allogenic muscle stem cells induces long-term muscle repair and clinical efficacy in Duchenne muscular dystrophy dogs. (medecinesciences.org)
Therefore, we sought to translate these results to a clinically relevant canine-to-canine allogeneic transplant model for Duchenne muscular dystrophy (DMD) and determine if CXCR4 is important for donor cell engraftment. (biomedcentral.com)
Intramuscular injection of donor muscle-derived cells into chimeric cxmd recipients restored dystrophin expression for at least 24 weeks in the absence of post-transplant immunosuppression, indicating that cell transplantation may be a viable therapeutic option for muscular dystrophy. (biomedcentral.com)

Moreover, SI221 decreased in vitro cell migration and invasion and reduced tumor growth in a RMS xenograft model. (oncotarget.com)

Findings The present research exposed that EZH2 affects many elements of South carolina biology, including self-renewal, expansion, and cell identification. (cancer-ecosystem.com)

HGF is produced and secreted by adjacent stromal and mesenchymal cells, it contributes to the development of epithelial organs in a paracrine fashion, exerts regenerative effects on epithelia in the liver, kidney, lung, and other tissues, and promotes the regression of fibrosis in numerous organs ( 7 , 8 ). (spandidos-publications.com)
Aldehyde dehydrogenase activity promotes survival of human muscle precursor cells. (medecinesciences.org)
Promotes differentiation of myogenic cells. (nih.gov)
It is a component of a cell-surface receptor complex that mediates cell-cell interactions between muscle precursor cells, and promotes myogenic differentiation. (nih.gov)
Loss of PRDM16 from brown fat precursors causes a loss of brown fat characteristics and promotes muscle differentiation. (bioseek.eu)

Ezh2 impacts South carolina expansion and difference We examined the South carolina human population by quantifying AZD8330 manufacture Pax7+ cells in G8 rodents, when SCs are extremely proliferative. (cancer-ecosystem.com)

Therefore, we complemented our investigations into the cellular development of the skeletal muscle of neonatal piglets with in vitro studies using a primary porcine myogenic cell culture model with Gln supplementation. (nature.com)
The primary purpose of this study is to determine whether breast cancer tumors respond (as measured by pathologic complete response: the absence of microscopic evidence of invasive tumor cells in the breast) to combined chemotherapy of AC(doxorubicin and cyclophosphamide) followed by paclitaxel plus trastuzumab or lapatinib or both given before surgery to patients with HER2-positive breast cancer. (stanford.edu)
SI221, which showed to be mainly effective against the SFK member YES, significantly reduced cell viability and induced apoptosis, without affecting non-tumor cells, such as primary human skin fibroblasts and differentiated C2C12 cells. (oncotarget.com)

These cells, theoretically, would not require transfection of dystrophin, and could potentially provide an autologous source of cells for transplant therapies. (umn.edu)

Nonetheless, the enhanced myogenesis leads to the formation of an extensive network of contractile myofibers, and this enhanced myogenesis can be conferred upon myogenic cells from clonal populations, such as G8 or C2C12 cells, if they are co-cultured with the limb mesenchymal cells. (biologists.com)
Ambike S, Penedo T, Kulkarni A, Cho H, Rietdyk S, Ambike S (2023) The sum of all connections are used in the cells were treated with an obstacle compared to cells in meiosis as it is relevant to models that treat high-impact frailty indicators implies that the MOSAP for human gait arise when extending this logic to (1) different adaptive gait tasks and populations suggests that the. (ruskinhouse.org)

Myogenic precursor cells in craniofacial muscles. (umn.edu)
NC cells differentiate to form a broad array of derivatives, including neurons and glia of the peripheral nervous system, smooth muscle, cartilage, craniofacial mesenchyme, and melanocytes ( Mayor and Theveneau, 2013 ). (jneurosci.org)

Intramuscular transplantation of myogenic cells in primates: importance of needle size, cell number, and injection volume. (medecinesciences.org)
Autologous transplantation of muscle-derived CD133 + stem cells in Duchenne muscle patients. (medecinesciences.org)
Transplantation of myogenic stem cells possesses great potential for long-term repair of dystrophic muscle. (biomedcentral.com)
In this study, we used a canine-to-murine xenotransplantation model to quantitatively compare canine muscle cell engraftment, and test the most effective cell population and modulating factor in a canine model of DMD using allogeneic transplantation experiments. (biomedcentral.com)
Furthermore, the canine-to-murine xenotransplantation model accurately predicted results in canine-to-canine muscle cell transplantation. (biomedcentral.com)
Yet, it remains unclear from murine transplantation experiments which cell population most effectively engrafts into diseased skeletal muscle. (biomedcentral.com)

Fracture Healing in Diabetes Fracture repair entails recruitment of mesenchymal stem cells after which differentiation to chondrocytes that type cartilage (see Chapter 12). (dnahelix.com)
A perivascular origin for mesenchymal stem cells in multiple human organs. (medecinesciences.org)
Both epithelial and mesenchymal components of carcinosarcoma show high-grade malignant cells. (medscape.com)

MAPK12 is required for the normal kinetochore localization of PLK1, prevents chromosomal instability and supports mitotic cell viability. (idrblab.net)

Background: HOX genes encode a family of homeodomain-containing transcription factors involved in the determination of cell fate and identity during embryonic development. (123dok.net)

Using various assays like RNA sequencing, cell staining and imaging, and molecular interaction and pathway analysis, the researchers investigated iSN04's effect on murine ESCs and iPSCs. (shinshu-u.ac.jp)

More recently, his group invented tools for cleavage-free large gene insertion via mining microbial recombination protein, and developed single-cell tracking approach for studying cancer biology and cancer immunology. (stanford.edu)
NADH dehydrogenase (ubiquinone) iron-sulfur protein 8 (Ndufs8), a subunit of NADH dehydrogenase in mitochondrial complex I, significantly increased in SOL-derived MBs compared with that in TA-derived cells. (bvsalud.org)
These new satellite cells will then fuse with the existing muscle fibers and add their nuclei to the cells to support greater protein synthesis and increase muscle hypertrophy or repair. (humankinetics.com)

Myogenesis is a multi-step process through which uncommitted mesodermal cells commit, differentiate and undergo profound phenotypic changes. (biologists.com)
While responses of adult stem cells are regulated extrinsically and age‐specifically, our work recently published puts forward experimental evidence suggesting that embryonic cells have an intrinsic youthful barrier to aging and produce soluble pro‐regenerative proteins that signal the MAPK pathway for rejuvenating myogenesis. (ca.gov)

Unfortunately, regenerative medicine is not yet feasible outside a laboratory setting as available protocols to generate target cells are complicated and expensive. (shinshu-u.ac.jp)
Additional recently published work was focused on developing new strategies for providing new source of regenerative cells to people who suffer from genetic myopaties (where their own muscle stem cells become exhausted due to the progression of the disease). (ca.gov)
Satellite television cells (SCs) sustain muscle development and empower adult skeletal muscle with strenuous regenerative capabilities. (cancer-ecosystem.com)

We previously reported that EZH2 inhibition induces cell cycle arrest followed by myogenic differentiation of RMS cells of the embryonal subtype (eRMS). (figshare.com)
This early investigation seeks to better characterize a potentially important cell population which has not been previously described. (umn.edu)
The HOXD9 gene was highly expressed in a side population (SP) of SK-MG-1 cells that was previously identified as an enriched-cell fraction of glioma cancer stem-like cells. (123dok.net)

Immortalized Canine Dystrophic Myoblast Cell Lines for Development of Peptide-Conjugated Splice-Switching Oligonucleotides. (institut-myologie.org)
We hope to identify a new source of cells for use in myoblast transfer therapy. (umn.edu)
By describing a means for EOM sparing in DMD, we may advance our understanding of the disease and identify a new source of cells for myoblast transfer therapy. (umn.edu)
IFN- expression is at basal levels in wholesome muscle tissues, although increases just after injury, peaking at day 5 post-injury corresponding to immune cell and myoblast infiltration. (rockinhibitor.com)

We, therefore, evaluated whether miR-101 is de-regulated in eRMS and investigated its interplaying with EZH2 as well as its role in the in vitro tumorigenic potential of these tumor cells. (figshare.com)
Results Herein, we report that miR-101 is down-regulated in eRMS patients and in tumor cell lines compared to their controls showing an inverse pattern of expression with EZH2. (figshare.com)
We also show that miR-101 is up-regulated in eRMS cells following both genetic and pharmacological inhibition of EZH2. (figshare.com)
In turn, miR-101 forced expression reduces EZH2 levels as well as restrains the migratory potential of eRMS cells and impairs their clonogenic and anchorage-independent growth capabilities. (figshare.com)
Finally, EZH2 recruitment to regulatory region of miR-101-2 gene decreases in EZH2-silenced eRMS cells. (figshare.com)
This phenomenon is associated to reduced H3K27me3 levels at the same regulatory locus, indicating that EZH2 directly targets miR-101 for repression in eRMS cells. (figshare.com)
Rodents with conditional mutilation of Ezh2 in Pax7-produced muscle mass precursor cells possess decreased muscle mass mass with smaller sized myofibers was selectively ablated in Pax7-produced cells by traversing knock-in rodents articulating Cre recombinase from the Pax7 locus (alleles (Su et al. (cancer-ecosystem.com)
Certainly, when Ezh2 was erased in dedicated myogenic precursors (MyoD+), gene misexpression was hardly noticed (Supplemental Fig. H5G). (cancer-ecosystem.com)

The intent of vaccination is to induce a combined antibody and T-cell anti-HER-2 immune response, which is intended to target HER-2-expressing tumor cells, and may induce tumor regression or slow progression of disease. (stanford.edu)
RATIONALE: Drugs used in chemotherapy use different ways to stop tumor cells from dividing so they stop growing or die. (stanford.edu)

These cells from EOM are more resistant to apoptosis than similar cells from limb muscles. (umn.edu)
Western blotting and immunofluorescence microscopy have been used to assay the roles of BCAS2 within the DSBs of PCa cells and apoptosis in Drosophila, respectively. (phylocommons.org)

In Drosophila , a population of muscle-committed stem-like cells called adult muscle precursors (AMPs) keeps an undifferentiated and quiescent state during embryonic life. (biologists.com)

However, whether the functionality of myogenic cells in adult muscles is attributed to the muscle fiber in which they reside and whether the characteristics of myogenic cells derived from slow- and fast-type fibers can be distinguished at the genetic level remain unknown. (bvsalud.org)

We utilized small molecule inhibitors to dedifferentiate muscle into dividing myogenic cells, without gene over expression, which is clinically adaptable. (ca.gov)

Of particular importance has been the identification of two families of transcription factors: the myogenic regulatory factors (MRFs) and the myocyte enhancer factors (MEFs). (biologists.com)
This function is independent of its catalytic activity and could affect mRNA processing and/or gene transcription to aid cell adaptation to osmolarity changes in the environment. (idrblab.net)

Body and muscle mass mass had been significantly decreased in mutilation in skeletal myogenic cells. (cancer-ecosystem.com)

This highly malignant tumor has traditionally been considered to arise from committed skeletal muscle precursor cells that fail to differentiate [ 1 ], although some RMSs develop in organs lacking a skeletal muscle component [ 2 , 3 ]. (oncotarget.com)

Anatomy70

Organisms24

Diseases5

Chemicals and Drugs78

Analytical, Diagnostic and Therapeutic Techniques and Equipment26

Psychiatry and Psychology1

Phenomena and Processes51

Disciplines and Occupations1

Information Science3

Activity of Myogenic Precursor Cells1

Proliferation17

Progenitor10

Myoblasts8

Normal myogenic cells2

Mpcs1

Pluripotent stem10

Stem cell20

Differentiate into skeletal muscle1

Limb bud2

Regeneration12

Mice4

Gene expression2

Inhibit1

Embryonic stem3

MYF54

Vivo6

Lineage4

Tissue4

Vertebrate4

Macrophages3

Muscular5

Migration1

Self-renewal1

Promotes5

Proliferative1

Primary3

Dystrophin1

Populations2

Craniofacial2

Transplantation6

Mesenchymal3

Viability1

Genes1

ESCs1

Protein3

Myogenesis2

Regenerative3

Previously3

Myoblast4

EZH28

Tumor cells2

Apoptosis2

Adult muscle1

Characteristics1

Inhibitors1

Transcription2

Significantly decreased1

Arise1