Myogenic precursor cell skeletal muscle cells progenitor satellite cell. Medical search. Frequent questions

Gros J, Manceau M, Thomé V, Marcelle C. A common somitic origin for embryonic muscle progenitors and satellite cells. (scielo.br)
Satellite cells from your trunk and the limb are derived from an embryonic populace of progenitor cells in the somites transient mesodermal constructions that develop on either part of the neural tube [1]. (healthyguide.info)
Interestingly recent data indicated that although MyoD is not indicated in quiescent satellite cells in the adult it is transiently indicated in satellite cell progenitors in the embryo suggesting that satellite cells are derived from committed embryonic precursors of myogenic lineage [4] [5]. (healthyguide.info)
In 2009 beginning of 2010 we have focused on investigating what factors human embryonic stem cells (hESCs) may produce that enhance regeneration and if those factors have any effects by themselves on regeneration. (ca.gov)
Our work is at the stage of understanding the molecular mechanisms by which the aging of the regenerative potential of organ stem cells can be reversed by particular human embryonic factors that are capable of neutralizing the affects of aged niches on tissue regenerative capacity. (ca.gov)
Skoglund G, Lainé J, Darabi R , Fournier E, Perlingeiro R, Tabti N. Physiological and ultrastructural features of human induced pluripotent and embryonic stem cell-derived skeletal myocytes in vitro. (uh.edu)
Assessment of the myogenic stem cell compartment following transplantation of pax3/pax7-induced embryonic stem cell-derived progenitors. (uh.edu)
Embryonic myogenic progenitor cells express CXCR4, G-protein coupled cell surface receptor, and migrate towards regions of SDF-1 expression during limb muscle development, suggesting that CXCR4/SDF-1 plays a role in muscle cell homing. (biomedcentral.com)
In Drosophila , a population of muscle-committed stem-like cells called adult muscle precursors (AMPs) keeps an undifferentiated and quiescent state during embryonic life. (biologists.com)
The embryonic AMPs are at the origin of all adult fly muscles and, as we demonstrate here, they express repressors of myogenic differentiation and targets of the Notch pathway known to be involved in muscle cell stemness. (biologists.com)

To determine their possible roles in tissues and satellite cells in vitro, their expression pattern was examined in tissues from 40-day-old chickens and in satellite cells from the breast muscles of 1-week-old and 2-week-old chickens using RT-PCR and immunocytochemistry analyses. (scielo.br)
Alameddine HS, Dehaupas M, Fardeau M. Regeneration of skeletal muscle fibers from autologous satellite cells multiplied in vitro. (medecinesciences.org)
Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro. (medecinesciences.org)
In vivo , the identification of macrophage phenotype is complicated since macrophages are obviously exposed to a microenvironment that is more complex respect to cell culture conditions, and they display characteristics that do not conform to the in vitro defined phenotypic categories. (hindawi.com)
We have chosen to work on human precursors, either in vitro or in vivo, in xenograft models that we have developed in successive European networks. (institut-myologie.org)
Satellite cells are in general easy to isolate from explanted skeletal muscle, and presumably muscle biopsies from an adult mammal would provide an ample supply of cells committed to the myogenic developmental pathway to facilitate the in vitro engineering of functional skeletal muscle. (josam.org)
To study the capacity of satellite cells to form into functional skeletal muscle tissue de novo in culture, we have developed several in vitro tissue models. (josam.org)
Myooids were activated by transverse electrical fields in a manner similar to that used to activate whole skeletal muscles in vitro during standard physiological measures of muscle function [8] . (josam.org)
Overall, Gln supplementation stimulated cell proliferation in muscle tissue and in vitro in myogenic cell culture, whereas muscle growth regulatory genes were barely altered. (nature.com)
Therefore, we complemented our investigations into the cellular development of the skeletal muscle of neonatal piglets with in vitro studies using a primary porcine myogenic cell culture model with Gln supplementation. (nature.com)
Our experiments will use in vitro cell culture including activated caspase and stress-induced death assays. (umn.edu)
To assess the differentiation capabilities of these cells, we will use flow cytometry to sort the CD34+ population, followed by in vitro differentiation assays using various growth factors and serum deprivation. (umn.edu)

Obestatin signalling counteracts glucocorticoid-induced skeletal muscle atrophy via NEDD4/KLF15 axis. (institut-myologie.org)
On the other hand, despite the atrophy and reduced myogenic potential of the EDL muscles in old rats, when an EDL muscle is cross-age transplanted from an old donor rat into a young host rat, the muscle regenerates as much mass and has as high a capacity for force development as a young muscle transplanted into a young host [4] . (josam.org)
Our hypothesis is that the EOM are spared from the degenerative effects of Duchenne muscular dystrophy (DMD) due to the presence of higher numbers of a unique precursor cell capable of maintaining a normal phenotype under conditions that normally result in atrophy. (umn.edu)
Defective muscle regeneration can severely affect re-innervation by motor axons, and neuromuscular junctions (NMJs) development, ultimately leading to skeletal muscle atrophy. (biomed.news)

The proliferation and differentiation of satellite cells are regulated by a number of extracellular signals ( Wang & Rudnicki, 2012 Wang YX, Rudnicki MA. (scielo.br)
Our team has demonstrated some important differences between man and mouse, in the regulation of the proliferation of myogenic precursors. (institut-myologie.org)
WAT is characterised by its capacity to adapt and expand in response to surplus energy through processes of adipocyte hypertrophy and/or recruitment and proliferation of precursor cells in combination with vascular and extracellular matrix remodelling. (springer.com)
Because of the decreased proliferation potential [9] and lower absolute number of satellite cells in muscles from the aged rats compared with adult rats [1,10,11] , one may hypothesize that the aged rats would not yield enough myoblasts to produce myooids in culture. (josam.org)
Proliferating, BrdU-positive cells in muscle sections were detected with immunohistochemistry indicating different cell types and decreasing proliferation with age. (nature.com)
More proliferation was observed in muscle tissue of LBW-GLN than LBW-ALA piglets at 5 dpn, but there was no clear effect of supplementation on related gene expression. (nature.com)
Cell culture experiments indicated that Gln could promote cell proliferation in a dose dependent manner, but expression of myogenesis regulatory genes was not altered. (nature.com)
However, diprotin A, a positive modulator of CXCR4-SDF-1 binding, significantly enhanced engraftment and stimulated sustained proliferation of donor cells in vivo . (biomedcentral.com)
Diprotin A stimulation of CXCR4, however, significantly increased the number of canine dystrophin-positive muscle fibers and canine-derived satellite cells by enhancing donor cell proliferation. (biomedcentral.com)

a reverse function mutant Talniflumate of Nkx3.2 blocks the ability of Sox9 to both inhibit myogenesis and induce chondrogenesis suggesting that Nkx3.2 is required for Sox9 to promote chondrogenic differentiation in satellite cells. (healthyguide.info)
Nonetheless, the enhanced myogenesis leads to the formation of an extensive network of contractile myofibers, and this enhanced myogenesis can be conferred upon myogenic cells from clonal populations, such as G8 or C2C12 cells, if they are co-cultured with the limb mesenchymal cells. (biologists.com)
Myogenesis is a multi-step process through which uncommitted mesodermal cells commit, differentiate and undergo profound phenotypic changes. (biologists.com)
The past two decades has seen major advances towards our current understanding of skeletal myogenesis (reviewed in Perry and Rudnicki, 2000 ). (biologists.com)
Other NIH-funded investigations in our lab explore epigenetic mechanisms through which an anti-aging protein called Klotho affects myogenesis and muscle regeneration in neonatal and aging muscle. (ucla.edu)

Mesodermal iPSC-derived progenitor cells functionally regenerate cardiac and skeletal muscle. (medecinesciences.org)

Cell Stem Cell 2013;13(5):590-601. (scielo.br)
Muscle satellite cells make up a stem cell populace that is capable of differentiating into myocytes and contributing to muscle mass regeneration upon injury. (healthyguide.info)
Stem cell function, self-renewal, and behavioral heterogeneity of cells from the adult muscle satellite cell niche. (medecinesciences.org)
These include generation of knock-in reporter stem cell lines to study myogenic differentiation, CRISPR/Cas9 gene targeting methods to correct mutated genes in muscular dystrophies, and using bioscaffolds, seeded with stem cells, to treat skeletal muscle injuries. (uh.edu)
Wu J, Matthias N, Lo J, Ortiz-Vitali JL, Shieh AW, Wang SH, Darabi R . A Myogenic Double-Reporter Human Pluripotent Stem Cell Line Allows Prospective Isolation of Skeletal Muscle Progenitors. (uh.edu)
Stem Cell Res. (uh.edu)
Wu J, Hunt SD, Matthias N, Servián-Morilla E, Lo J, Jafar-Nejad H, Paradas C, Darabi R . Generation of an induced pluripotent stem cell line (CSCRMi001-A) from a patient with a new type of limb-girdle muscular dystrophy (LGMD) due to a missense mutation in POGLUT1 (Rumi). (uh.edu)
Stem Cell Research. (uh.edu)
Matthias N, Hunt SD, Wu J, Darabi R . Skeletal muscle perfusion and stem cell delivery in muscle disorders using intra-femoral artery canulation in mice. (uh.edu)
The rapid advance of stem cell clinical trials for a broad spectrum of conditions warrants an update of the review by Trounson (2009) [ 1 ]. (biomedcentral.com)
There has been a rapid surge in clinical trials involving stem cell therapies over the last two to three years and those trials are establishing the clinical pathways for an emergent new medicine. (biomedcentral.com)
Salk Institute researchers are finding methods to speed up methods to accelerate the regeneration of muscle tissue, utilizing a combination of molecular compounds that are generally utilized in the stem-cell analysis. (sciencetrend.ca)
In lab analysis, they're used to transform specialized cells, like skin cells, into extra stem-cell-like cells that are pluripotent, suggesting they have the ability to become many various kinds of cells. (sciencetrend.ca)
In this research, the team used two completely different mouse models to pinpoint the muscle stem-cell-specific or niche-specific modifications following the addition of Yamanaka factors. (sciencetrend.ca)
In vivo partial reprogramming of myofibers promotes muscle regeneration by remodeling the stem cell niche. (sciencetrend.ca)
Therefore, these results establish the efficacy of diprotin A in stimulating muscle cell engraftment, and highlight the pre-clinical utility of a xenotransplantation model in assessing the relative efficacy of muscle stem cell populations. (biomedcentral.com)
We used a clinically acceptable regimen of hematopoietic stem cell transplantation to establish mixed donor/host blood cell chimerism and immune tolerance in a canine model of Duchenne muscular dystrophy ( cxmd ) [ 9 ]. (biomedcentral.com)
From a research, medical, and business standpoint, mesenchymal stem cell (MSC)-based therapies are fascinating. (biomedcentral.com)

MicroRNA-133 controls brown adipose determination in skeletal muscle satellite cells by targeting Prdm16. (scielo.br)
The epidemic of obesity, closely associated with increases in diabetes, hypertension, hyperlipidemia, cancer and other disorders, has propelled a major interest in adipose cells and tissues. (bioseek.eu)
Adipose tissues contain two distinct types of fat cells, white and brown. (bioseek.eu)
There are many studies involving autologous therapies and some allogenic therapies, based on the recovery of mobilized bone marrow cells, including mesenchymal stem cells (MSCs) and adipose derived stem cells that also include the stromal or adherent cell type that has an MSC phenotype. (biomedcentral.com)
Here, we summarise the mechanisms contributing to adipose tissue (AT) plasticity and function including characteristics and cellular complexity of the various adipose depots and we discuss recent insights into AT origins, identification of adipose precursors, pathophysiological regulation of adipogenesis and its relation to WAT/BAT expandability in obesity and its associated comorbidities. (springer.com)
The 'adipose tissue expandability model' identifies the limited capacity and dysfunctionality of WAT, preventing its expansion and accommodation of surplus of energy, as key determinants for the onset and progression of obesity-associated metabolopathologies as a result of ectopic deposition of toxic lipid species in metabolic organs (i.e. muscle or liver [also known as lipotoxic insult]) [ 7 ]. (springer.com)
MSCs can be isolated from a range of tissues, but the most commonly cited sources are bone marrow (BM), adipose tissue, muscle, bone, and perinatal tissues (e.g. (biomedcentral.com)

For postnatal development and regeneration of skeletal muscle mass satellite television cells Mirtazapine a self-renewing pool of muscle mass stem cells give rise to child myogenic precursor cells that contribute to the formation of fresh muscle mass fibers. (mdm-inhibitors.com)
mutilation in Pax7-produced skeletal muscle mass AZD8330 manufacture cells outcomes in problems of postnatal muscle mass development characterized by decreased muscle mass mass with smaller sized muscle mass materials. (cancer-ecosystem.com)
Conditional Ezh2 mutilation outcomes in postnatal skeletal muscle mass problems and an impoverished South carolina pool. (cancer-ecosystem.com)
Postnatal growth and repair of skeletal muscle relies upon a population of quiescent muscle precursor cells, called satellite cells that can be activated to proliferate and differentiate into new myofibers, as well as self-renew to replenish the satellite cell population. (biomedcentral.com)
The delayed growth of LBW piglets is mainly due to a slower development of skeletal muscle as the myogenic activity is usually impaired in these animals during the fetal and early postnatal period 3 , 4 . (nature.com)

Fracture Healing in Diabetes Fracture repair entails recruitment of mesenchymal stem cells after which differentiation to chondrocytes that type cartilage (see Chapter 12). (dnahelix.com)
A perivascular origin for mesenchymal stem cells in multiple human organs. (medecinesciences.org)
While numerous teams continue to refine and expand the role of bone marrow and cord blood stem cells for their vanguard uses in blood and immune disorders, many others are looking to expand the uses of the various types of stem cells found in bone marrow and cord blood, in particular mesenchymal stem cells, to uses beyond those that could be corrected by replacing cells in their own lineage. (biomedcentral.com)
Adult mesenchymal stem cells (MSCs) represent a subject of intense experimental and biomedical interest. (biomedcentral.com)

Transplantation of genetically corrected human iPSC-derived progenitors in mice with limb-girdle muscular dystrophy. (medecinesciences.org)

One such alternative cell fate is the adipogenic fate as Pax7(+) satellite cells isolated from solitary myofibers used adipogenic fate in addition ARHGAP1 to muscle mass fate significance of these factors we used a mouse fracture healing model inside a genetically altered reporter mouse where muscle mass progenitors were lineage-traced. (healthyguide.info)
We found that in the descendents of muscle mass progenitors that contributed to cartilage formation Nkx3.2 and Sox9 were strongly induced while Pax3 manifestation was strongly repressed. (healthyguide.info)
non-committed originate cells and Pax7+/Myf5+ muscle mass progenitors. (cancer-ecosystem.com)

During development, one of the major lineages expressing Pax3 is the skeletal muscle lineage. (wikipedia.org)
Finally we found that in an mouse model of fracture healing where muscle mass progenitor cells were lineage-traced Nkx3.2 and Sox9 are significantly upregulated while Pax3 is significantly downregulated in the muscle mass progenitor cells that give rise to chondrocytes during fracture restoration. (healthyguide.info)
Here we show by in vivo fate mapping that brown, but not white, fat cells arise from precursors that express Myf5, a gene previously thought to be expressed only in the myogenic lineage. (bioseek.eu)
Taken together, these data indicate that PRDM16 specifies the brown fat lineage from a progenitor that expresses myoblast markers and is not involved in white adipogenesis. (bioseek.eu)
During the last several years, his lab has developed novel methods for multi-lineage differentiation of pluripotent stem cells for cell therapy in mice models of muscular dystrophies and injuries. (uh.edu)
More research teams are accelerating the use of other types of adult stem cells, in particular neural stem cells for diseases where beneficial outcome could result from either in-lineage cell replacement or extracellular factors. (biomedcentral.com)
The use of patient's own bone marrow aspirates, hematopoietic stem cells and MSCs, for heart muscle tissue repair can be puzzling because these cells do not normally contribute to the cardiac lineage types that are desired. (biomedcentral.com)
I have found a CD34+ group of cells in EOM that is negative for other lineage-specific markers. (umn.edu)

When skeletal muscle is injured due to physical or chemical insult, a pool of self-renewing muscle stem cells residing within the skeletal muscles, called satellite cells, can give rise to differentiated myofibers to repair injured muscle ( Charge & Rudinicki, 2004 Chargé SBP, Rudnicki MA. (scielo.br)
Isolation and culture of individual myofibers and their satellite cells from adult skeletal muscle. (scielo.br)
Rodents with conditional mutilation of Ezh2 in Pax7-produced muscle mass precursor cells possess decreased muscle mass mass with smaller sized myofibers was selectively ablated in Pax7-produced cells by traversing knock-in rodents articulating Cre recombinase from the Pax7 locus (alleles (Su et al. (cancer-ecosystem.com)
Transplanted myoblasts can migrate several millimeters to fuse with damaged myofibers in nonhuman primate skeletal muscle. (medecinesciences.org)
2011). Satellite cells are located in a distinct segment between a layer of connective tissue (basal lamina) and muscle fibers (myofibers). (sciencetrend.ca)

The Q+ and Q- isoforms of PAX3 are generally co-expressed in cells. (wikipedia.org)
Among the PAX3 target genes, there is one group associated with muscle development and a second group associated with neural and melanocyte development. (wikipedia.org)
Therefore our and analyses suggest that the balance of Pax3 Nkx3.2 and Sox9 may act as a molecular Talniflumate switch during the chondrogenic differentiation of muscle mass progenitor cells which may be important for fracture healing. (healthyguide.info)
Collectively our data suggest that the balance of Nkx3.2 Sox9 and Pax3 can act as a molecular switch during the chondrogenic differentiation of satellite cells which may play an important part in the healing process experiments were normalized to GAPDH. (healthyguide.info)
We demonstrate that mdPCR can give exact molecule counts per cell for mRNA isoforms encoding the myogenic transcription factor Pax3. (biomed.news)

Alterations in the TGFbeta signaling pathway in myogenic progenitors with age. (sciencetrend.ca)

Dystrophin expression in muscles of Duchenne muscular dystrophy patients after high-density injections of normal myogenic cells. (medecinesciences.org)
Human ES and iPS-Derived Myogenic Progenitors Restore Dystrophin and Improve Contractility upon Transplantation in Dystrophic Mice. (uh.edu)
Intramuscular injection of donor muscle-derived cells into chimeric cxmd recipients restored dystrophin expression for at least 24 weeks in the absence of post-transplant immunosuppression, indicating that cell transplantation may be a viable therapeutic option for muscular dystrophy. (biomedcentral.com)
These cells, theoretically, would not require transfection of dystrophin, and could potentially provide an autologous source of cells for transplant therapies. (umn.edu)

Intramuscular transplantation of myogenic cells in primates: importance of needle size, cell number, and injection volume. (medecinesciences.org)
Autologous transplantation of muscle-derived CD133 + stem cells in Duchenne muscle patients. (medecinesciences.org)
Transplantation of myogenic stem cells possesses great potential for long-term repair of dystrophic muscle. (biomedcentral.com)
In murine-to-murine transplantation experiments, CXCR4 expression marks a population of adult murine satellite cells with robust engraftment potential in mdx mice, and CXCR4-positive murine muscle-derived SP cells home more effectively to dystrophic muscle after intra-arterial delivery in mdx 5cv mice. (biomedcentral.com)
In this study, we used a canine-to-murine xenotransplantation model to quantitatively compare canine muscle cell engraftment, and test the most effective cell population and modulating factor in a canine model of DMD using allogeneic transplantation experiments. (biomedcentral.com)
Furthermore, the canine-to-murine xenotransplantation model accurately predicted results in canine-to-canine muscle cell transplantation. (biomedcentral.com)
Yet, it remains unclear from murine transplantation experiments which cell population most effectively engrafts into diseased skeletal muscle. (biomedcentral.com)
In clinical practice, these involve the life-long immunosuppression of the organ transplantation patients, the creation of the supporting environment for engineered tissues in the mended organ, the unsolved issues of cell survival and differentiation of the cell-based therapy, and the selection and development of vectors for gene therapy. (biomedcentral.com)

These cells lay beneath the basement membrane of the muscle mass fiber and are usually mitotically quiescent [1]. (healthyguide.info)
This is unlike the satellite cells of limb muscles, which remain quiescent unless injury is sustained. (umn.edu)
For example, muscle stem cells (MuSCs), located beneath the basal lamina, exist in the quiescent state but can transition to an activated, proliferative state upon injury. (biomed.news)

Derivation of Skeletal Myogenic Precursors from Human Pluripotent Stem Cells Using Conditional Expression of PAX7. (uh.edu)

What was surprising is that administering these inhibitors to the whole animal appears to reduce TGF-beta levels in the whole animal, suggesting some kind of feed-back and perhaps effects on other tissues as well as muscle. (ca.gov)
The poor performance of stem cells in an aged organ, such as skeletal muscle, is caused by the changes in regulatory pathways such as Notch, MAPK and TGF‐β, where old differentiated tissues and blood circulation inhibit the regenerative performance of organ stem cells. (ca.gov)
Feeding with amino acids can stimulate protein synthesis in all tissues, especially in skeletal muscle as reviewed by Davis et al. (nature.com)
Originally discovered as axon guidance factors, they have been implicated in cancer progression, embryonal organogenesis, skeletal muscle innervation, and other physiological and developmental functions in different tissues. (biomed.news)
Therefore, we propose that damage to and subsequent release of mtDNA elicits a protective signalling response that enhances nDNA repair in cells and tissues, suggesting that mtDNA is a genotoxic stress sentinel. (regenerativemedicine.net)

Günther S, Kim J, Kostin S, Lepper C, Fan CM, Braun T. Myf5-positive satellite cells contribute to Pax7-dependent long-term maintenance of adult muscle stem cells. (scielo.br)
2009). We produced rodents in AZD8330 manufacture which was conditionally ablated in SCs (muscle mass knockout, mouse, where cells that communicate or possess ever indicated Myf5-Cre are YFP+, while cells that possess by no means indicated Myf5-Cre are YFP? (cancer-ecosystem.com)
A even more unsuspecting and plastic material chromatin environment, such as that of Sera cells or non-committed Pax7+/Myf5? (cancer-ecosystem.com)
Wu J, Hunt SD, Xue H, Liu Y, Darabi R . Generation and Characterization of a MYF5 Reporter Human iPS Cell Line Using CRISPR/Cas9 Mediated Homologous Recombination. (uh.edu)

In 2010 beginning of 2011, we have approached the identification and characterization of the proteins that are produced by hESCs and have the rejuvenating and pro-regenerative activity on adult muscle. (ca.gov)

Upon activation satellite cells rapidly initiate MyoD expression which leads to the activation of myogenin and terminally differentiated structural muscle mass genes such as myosin heavy chain (MHC) [1] [3]. (healthyguide.info)
Skeletal muscle myosin heavy chain (MHC) content was measured and MHC isoforms were separated on SDS-PAGE gels and quantified. (josam.org)
Specifically,treatment of primary limb bud cultures with p38 inhibitors increases the expression of myogenic markers and causes a striking increase in formation of myotubes, which were detected using antibodies specific for myosin heavy chain. (biologists.com)

The transcription factor CCAAT/enhancer binding protein beta (C/EBPβ) is expressed in Pax7 + satellite cells of healthy muscle and is downregulated during myoblast differentiation. (biomedcentral.com)
When overexpressed in the murine myoblast cell line C2C12 or in primary myoblasts, C/EBPβ reduced MyoD and other myogenic protein levels during differentiation, in addition to reducing myoblast fusion. (biomedcentral.com)
Immortalized Canine Dystrophic Myoblast Cell Lines for Development of Peptide-Conjugated Splice-Switching Oligonucleotides. (institut-myologie.org)
We hope to identify a new source of cells for use in myoblast transfer therapy. (umn.edu)
By describing a means for EOM sparing in DMD, we may advance our understanding of the disease and identify a new source of cells for myoblast transfer therapy. (umn.edu)

Adult and aged rat myooids had only ~35-60% of the skeletal muscle MHC content of control skeletal muscle from rats, the remaining MHC content consisting of isoforms found in cultured fibroblasts but not in control skeletal muscle. (josam.org)
In addition, myooids expressed only developmental isoforms of skeletal muscle MHC, known to generate less specific force than adult isoforms. (josam.org)
Quantifying the exact expression levels of the mRNA isoforms encoding these myogenic transcription factors is therefore key to understanding how MuSCs switch between cell states. (biomed.news)

The analysis offers perception into the underlying mechanisms associated with muscle regeneration and growth and will help athletes and aging adults regenerate tissue more effectively. (sciencetrend.ca)
I will perform the following experiments: 1) examine the phenotype and differentiation potential of these cells, 2) determine the mechanism for their resiliency to injury, and 3) examine their ability to repair or regenerate muscle in vivo . (umn.edu)
Skeletal muscle is characterized by a remarkable capacity to rearrange after physiological changes and efficiently regenerate. (biomed.news)

Specifically, our data suggest that several other ligands of MAPK pathway secreted by hESCs are likely to enhance and rejuvenate the regeneration of old muscle tissue. (ca.gov)
We have submitted the several manuscripts on topics of enhanced tissue regeneration and we are preparing the manuscript that identifies hESC-based novel strategies for restoring high regenerative capacity to old muscle. (ca.gov)
Finally, our data suggest that muscle stem cells either do not accumulate DNA damage with age or can efficiently repair such damage, when activated for tissue regeneration. (ca.gov)
Thus, the use of hESC-produced pro-regenerative factors for boosting the regenerative capacity of organ stem cells is likely to yield healthy, young tissue. (ca.gov)
Although functional organ stem cells persist in the old, tissue damage invariably overwhelms tissue repair, ultimately causing the demise of an organism. (ca.gov)
In this paper, we discuss the current available information about the role that different phenotypes of macrophages plays after injury and during the remodelling phase in different tissue types, with particular attention to the skeletal muscle. (hindawi.com)
The balance between differentiation and self-renewal is critical to maintain muscle tissue homeostasis, and alterations in this equilibrium can lead to chronic muscle degeneration. (biomedcentral.com)
These early trials are showing roles for stem cells both in replacing damaged tissue as well as in providing extracellular factors that can promote endogenous cellular salvage and replenishment. (biomedcentral.com)
In recent research, scientists managed to activate the precursors of muscle cells, which contributes to aiding muscular regeneration and rebuilding damaged tissue. (sciencetrend.ca)
One of the great advantages of tissue engineering of skeletal muscle is that adult skeletal muscle contains many myogenic precursor stem cells, termed "satellite cells", which are involved in the repair and regeneration of skeletal muscle throughout the lifespan. (josam.org)
Prior to tissue engineering of functional skeletal muscle from satellite cells harvested from aged mammals, two key questions must be addressed. (josam.org)
The purpose of the current study was to compare the excitability and contractility of myooids engineered from skeletal muscles of 32-month-old rats with that of myooids engineered from adult rat tissue. (josam.org)
Glutamine is regarded as the most abundant amino acid in the body, is mainly synthesized in skeletal muscle 10 , and muscle tissue is the most important site for Gln storage 11 . (nature.com)
The compromised tissue repair program is attributable to the gradual depletion of stem cells and to altered regulatory signals. (biomed.news)
Adult stem cells play key roles in homeostasis and tissue repair. (biomed.news)
The response of target cells is further regulated by their microenvironment, involving the extracellular matrix, which may be modified by MSC-produced matrix metalloproteinases (MMPs) and tissue inhibitor of MMPs. (biomedcentral.com)
MSCs can be defined by their ability to adhere to tissue culture plastic, their expression of several cell surface molecular epitopes-cluster of differentiation CD73, CD90, and CD105, and others-as well as their lack of several surface markers, including CD45 [ 6 ]. (biomedcentral.com)

We also demonstrate that the transcriptional regulator PRDM16 (PRD1-BF1-RIZ1 homologous domain containing 16) controls a bidirectional cell fate switch between skeletal myoblasts and brown fat cells. (bioseek.eu)
Conversely, ectopic expression of PRDM16 in myoblasts induces their differentiation into brown fat cells. (bioseek.eu)

Inducible depletion of satellite cells in adult, sedentary mice impairs muscle regenerative capacity without affecting sarcopenia. (scielo.br)
Identification of a novel population of muscle stem cells in mice: potential for muscle regeneration. (medecinesciences.org)
After a second round of injury, muscle regeneration is impaired in C/EBPβ conditional knockout mice compared to wild-type control mice. (biomedcentral.com)
2021), the investigators confirmed that utilizing these compounds increased the regeneration of muscle cells in mice by activating the precursors of muscle cells, known as myogenic progenitors. (sciencetrend.ca)
Within the myofiber-specific model, they discovered that adding the Yamanaka factors accelerated muscle regeneration in mice by decreasing the levels of a protein referred to as Wnt4 in the area of interest, which activated the satellite cells. (sciencetrend.ca)
Indeed, CXCR4-positive muscle-derived side population (SP) cells home more effectively to dystrophic muscle after intra-arterial delivery in mdx 5cv mice [ 10 ]. (biomedcentral.com)
This population is present in limb muscles of neonatal mice, but is only maintained throughout adulthood in EOM. (umn.edu)
These cells are also present in the EOM of mdx mice and mdx/utrophin -/- mice, models for DMD. (umn.edu)
Ultimately, we hope to transplant our EOM-derived progenitor cells into injured limb muscle of mdx mice in order to determine the regenerative capabilities in vivo . (umn.edu)
To facilitate gene and cell therapy experiments, we created severely immune-deficient mouse models of Duchenne muscular dystrophy (DMD), limb girdle muscular dystrophy 2B (LGMD2B), and limb girdle muscular dystrophy 2D (LGMD2D) by crossing mdx 4Cv , Bl/AJ, and Sgca-null mice with NRG immune-deficient mice. (plos.org)
We performed centronucleation, Evans blue dye, hydroxyproline, and treadmill assays on the disease model mice versus NRG controls to evaluate muscle histology and function. (plos.org)
These studies demonstrated that the mdx 4Cv /NRG and Sgca/NRG mice showed significant deficits in muscle structure and function in all the assays and were similar to each other. (plos.org)

Duchenne muscular dystrophy (DMD) is the most common form of muscular dystrophy, characterized by widespread degeneration of the skeletal, respiratory, and cardiac muscles, resulting in disability and premature death. (plos.org)

Furthermore, Gln serves as a precursor for the synthesis of arginine, which is indispensable for the optimal growth of neonatal piglets 14 and plays important roles in essential metabolic pathways 15 . (nature.com)

Finally, Prdm16-deficient brown fat displays an abnormal morphology, reduced thermogenic gene expression and elevated expression of muscle-specific genes. (bioseek.eu)
Expression of muscle growth related genes was quantified with qPCR. (nature.com)
These novel immune-deficient mouse models of DMD, LGMD2B, and LGMD2D will be useful for long-term gene and cell therapy studies involving transfer of foreign genes and cells. (plos.org)
Muscular dystrophies are genetic diseases caused by mutations in genes encoding muscle proteins, leading to progressive muscle degeneration 1 . (plos.org)
Because of their monoallelic genetic basis, the addition of genes or cells that could provide the DMD, SGCA, or DYSF protein that is deficient in these disorders has the potential to be an effective treatment. (plos.org)
Gene therapy strategies to introduce functional DMD , SGCA , or DYSF genes to diseased muscle fibers are attractive approaches currently under development 5 , 6 , 7 , 8 , 9 . (plos.org)

Of particular importance has been the identification of two families of transcription factors: the myogenic regulatory factors (MRFs) and the myocyte enhancer factors (MEFs). (biologists.com)
The control of MuSC state depends on the expression levels of myogenic transcription factors. (biomed.news)

Direct isolation of satellite cells for skeletal muscle regeneration. (medecinesciences.org)

Satellite television cells (SCs) sustain muscle development and empower adult skeletal muscle with strenuous regenerative capabilities. (cancer-ecosystem.com)
However, discoveries in our lab have also shown that regulatory interactions between cytotoxic, M1 macrophages in dystrophic muscle and anti-inflammatory, M2 macrophages are important in regulating the balance between the death of dystrophic muscle and regenerative processes. (ucla.edu)
However, during aging, extensive injury, or pathological conditions, the complete regenerative program is severely affected, with a progressive loss of muscle mass and function, a condition known as sarcopenia. (biomed.news)

Cellular and molecular mechanisms regulating skeletal muscle development. (scielo.br)
Additionally, our data in progress suggest that muscle and brain age by similar molecular mechanisms and thus, therapeutic strategies for rejuvenating muscle repair might be applicable to the restoration of neurogenesis in aged brain. (ca.gov)
These cell lines are shared in collaboration with many international teams, either to study physiological or pathophysiological mechanisms, or to test innovative therapeutic approaches. (institut-myologie.org)
Laboratory work has centered on the mechanisms of how cells acquire specific fates during growth and development, providing insights into normal and abnormal mechanisms of growth control. (stanford.edu)
Exploring the mechanisms through which the immune system can modulate skeletal muscle wasting, injury, regeneration and growth is a particular focus of the lab. (ucla.edu)

The investigators are also studying different methods to rejuvenate cells, including utilizing mRNA and genetic engineering. (sciencetrend.ca)
Panobinostat treatment drastically repressed mRNA for DNMT1 and DNMT3a in the two cell lines whilst no adjustments have been observed in DNMT3b levels. (ilreceptor.com)

In the beginning satellite cells were considered to Talniflumate be unipotent stem cells with the ability of generating a unique specialized phenotype the skeletal muscle mass cells. (healthyguide.info)

Persistent expression of C/EBPβ upregulates Pax7, inhibits MyoD, and blocks myogenic differentiation. (biomedcentral.com)

The main interest of Dr. Darabi's lab is to use pluripotent stem cells for skeletal muscle regeneration in muscular dystrophies and muscle injuries. (uh.edu)
At the same time, the first three trials using cells derived from pluripotent cells have begun. (biomedcentral.com)

Loss of PRDM16 from brown fat precursors causes a loss of brown fat characteristics and promotes muscle differentiation. (bioseek.eu)
Aldehyde dehydrogenase activity promotes survival of human muscle precursor cells. (medecinesciences.org)
IL-2/IL2R also promotes the differentiation of T cells into effector T cells. (ilreceptor.com)
Here we show that, concurrent with a decrease in chondrogenesis, inhibition of p38 in limb bud cultures dramatically promotes muscle development. (biologists.com)

This includes bone and cartilage repair, cell types into which MSCs readily differentiate, and immune conditions such as graft versus host disease and autoimmune conditions that utilize the MSC's immune suppressive properties. (biomedcentral.com)
Therefore, in collaboration with W. Wright, we have developed an approach for the immortalisation of human muscle precursors that allows them to retain their ability to differentiate: this has enabled the generation of immortal cell lines derived from patient cells. (institut-myologie.org)

Cellular and molecular regulation of muscle regeneration. (scielo.br)
Recent mechanistic insights into the trophic activities of these cells point to ultimate regulation by nitric oxide, nuclear factor-kB, and indoleamine, among other signaling pathways. (biomedcentral.com)

LGMD2B leads to a later-onset, milder form of muscular dystrophy characterized by progressive degeneration of skeletal muscles and resulting disability 1 , 4 . (plos.org)

Studies previously conducted show that HS negatively affects the skeletal muscle growth and development by changing its effects on myogenic regulatory factors, insulin growth factor-1, and heat-shock proteins. (frontiersin.org)

Our team is working on the molecular and cellular actors involved in human muscle regeneration , in muscle ageing and in muscular dystrophies including oculopharyngeal muscular dystrophy (OPMD) and Duchenne muscular dystrophy (DMD). (institut-myologie.org)
We hypothesize that the remaining force deficit is due to cellular and sub-cellular disorganization-Myooids lack the density and organization of sarcomeric arrays seen in skeletal muscle. (josam.org)
This study elucidated effects of glutamine (Gln) supplementation on the cellular muscle development of LBW and normal birth weight (NBW) piglets. (nature.com)
We observed a temporarily increased intramuscular availability of free Gln that could have influenced cellular processes in skeletal muscle. (nature.com)

Pericytes of human skeletal muscle are myogenic precursors distinct from satellite cells. (medecinesciences.org)

Upon injury or when challenged with a variety of mechanical or biochemical stimuli satellite cells re-enter the cell cycle and give rise to Talniflumate differentiated myocytes which form new muscle mass materials or fuse with existing materials and contribute to muscle mass growth and restoration [1]. (healthyguide.info)

In living organisms, if the temperature exceeds the normal range (thermo-neutral zone), it disturbs the normal physiological functioning and induces cell injury. (frontiersin.org)

Defining the heterogeneity of skeletal muscle-derived side and main population cells isolated immediately ex vivo. (umn.edu)

They have proinflammatory functions: they produce effector molecules (reactive oxygen and nitrogen intermediates) and inflammatory cytokines (IL-1 β , TNF α , and IL-6) and participate as inducer and effector cells in polarized Th1 responses. (hindawi.com)
For example, our findings have shown that macrophages and eosinophils are key effector cells in the pathogenesis of Duchenne muscular dystrophy. (ucla.edu)

Cell Metabolism 2013;17(2):210-24. (scielo.br)
More precisely we are working on RNA metabolism , muscle regeneration , muscle stem cells , and fibrosis , with the final aim of developing innovative therapeutic approaches. (institut-myologie.org)

Findings The present research exposed that EZH2 affects many elements of South carolina biology, including self-renewal, expansion, and cell identification. (cancer-ecosystem.com)
These findings demonstrate that C/EBPβ is a novel regulator of satellite cell self-renewal during muscle regeneration acting at least in part through Notch2. (biomedcentral.com)
These findings had been corroborated by westernblot analysis displaying a powerful reduction of DNMT1 and DNMT3a protein in each cell lines but not of DNMT3b. (ilreceptor.com)

An absolute requirement for Pax7-positive satellite cells in acute injury-induced skeletal muscle regeneration. (sciencetrend.ca)
Discoveries in the our lab over the past 25 years have shown that immune cells, especially myeloid cells, play a major role in modulating muscle injury and repair that occur in chronic, muscle wasting diseases and following acute injuries. (ucla.edu)
We are also determining how those Klotho-driven epigenetic regulatory influences affect muscle growth following acute muscle injury or exercise. (ucla.edu)

Unlike additional research in which removal was conditionally acquired in dedicated progenitors or differentiated cells (Chen et al. (cancer-ecosystem.com)
Using genetic tools to conditionally abrogate C/EBPβ expression in Pax7 + cells, we examined the role of C/EBPβ in self-renewal of satellite cells during muscle regeneration. (biomedcentral.com)

Heparan sulfate proteoglycans (HSPGs) are present on the cell surface and in the extracellular matrix in all metazoans. (scielo.br)

Systemic delivery of allogenic muscle stem cells induces long-term muscle repair and clinical efficacy in Duchenne muscular dystrophy dogs. (medecinesciences.org)
Ongoing investigations in the lab are revealing the identity of specific molecules released by myeloid cells that promote muscular dystrophy. (ucla.edu)
The extraocular muscles (EOM) are well characterized as being uniquely spared in Duchenne muscular dystrophy (DMD). (umn.edu)

From a functional standpoint, PAX3c, PAX3d, and PAX3h stimulate activities such as cell growth whereas PAX3e and PAX3g inhibit these activities, and PAX3a and PAX3b show no activity or inhibit these endpoints. (wikipedia.org)
Functional three-dimensional skeletal muscle constructs (myooids) were engineered from myogenic cells harvested from the muscles of adult and aged rats. (josam.org)
First, to what extent are the satellite cells from aged mammals capable of fusion into functional myotubes, and second, do satellite cells from aged mammals have any form of impaired function when compared with those from young or adult mammals? (josam.org)
These changes include elongation, polarization, aggregation and fusion, which ultimately give rise to functional muscle. (biologists.com)

Upon increasing culture times in chicken´s primary skeletal muscle satellite cells, SULF1 and SULF2 expression in 1-week-old chickens was significantly higher than in 2-week-old chickens, suggesting that sulfatases play a key role in satellite cell development. (scielo.br)
Bober E, Franz T, Arnold HH, Gruss P, Tremblay P. Pax-3 is required for the development of limb muscles:a possible role for the migration of dermomyotomal muscle progenitor cells. (scielo.br)

Our current running hypothesis is that the positive growth factors produced by hESCs trigger injured muscle to initiate and maintain regeneration, the TGF-beta inhibitors produced by hESCs reduce the TGF-beta signaling, and the combination assures the robust regeneration of muscle. (ca.gov)
If healthy muscle progenitor cells were introduced and could fuse to existing deficient fibers, as well as form new muscle fibers, regeneration of muscle might occur 11 , 12 . (plos.org)

PRDM16 controls a brown fat/skeletal muscle switch. (bioseek.eu)

Developmental Cell, 42 (3). (uea.ac.uk)

With aging beyond adulthood, the pool of available satellite cells is known to decline [1-4] . (josam.org)

For Aim 4 we have examined the effects on live regenerating muscle of administering the TGF-beta inhibitors that we found in Aim 2. (ca.gov)

In addition, mtDNA stress in TFAM-deficient mouse melanoma cells produces tumours that are more resistant to doxorubicin in vivo. (regenerativemedicine.net)

Anatomy67

Organisms13

Diseases10

Chemicals and Drugs80

Analytical, Diagnostic and Therapeutic Techniques and Equipment30

Phenomena and Processes55

Disciplines and Occupations1

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture1

Information Science5

Health Care2

Embryonic10

Vitro12

Atrophy4

Proliferation9

Myogenesis5

Mesodermal1

Stem Cell18

Adipose7

Postnatal5

Mesenchymal stem4

IPSC-derived1

Mass progenitors3

Lineage8

Myofibers5

Pax35

Signaling pathway1

Dystrophin4

Transplantation8

Quiescent3

Human pluripotent stem1

Tissues5

Myf54

Adult muscle1

Myosin heavy3

Myoblast5

Isoforms3

Regenerate3

Tissue17

Myoblasts2

Mice12

Cardiac1

Neonatal1

Genes6

Transcription2

Isolation1

Regenerative3

Mechanisms5

MRNA2

Phenotype1

MyoD1

Pluripotent2

Promotes4

Differentiate2

Regulation2

Degeneration1

Regulatory factors1

Cellular4

Distinct1

Myocytes1

Physiological1

Heterogeneity1

Effector cells2

Metabolism2

Findings3

Acute3

Conditionally2

Extracellular matrix1

Muscular dystrophy3

Functional4

Development2

Regeneration of muscle2

Prdm161

Developmental1

Adulthood1

Inhibitors1

Vivo1