• Pax7, MyoD, myogenin, and IGF-1 expression patterns were upregulated after injury at both the mRNA and protein levels. (bvsalud.org)
  • Quantifying the exact expression levels of the mRNA isoforms encoding these myogenic transcription factors is therefore key to understanding how MuSCs switch between cell states. (biomed.news)
  • We demonstrate that mdPCR can give exact molecule counts per cell for mRNA isoforms encoding the myogenic transcription factor Pax3. (biomed.news)
  • In evolutionary developmental biology, Paired box (Pax) genes are a family of genes coding for tissue specific transcription factors containing an N-terminal paired domain and usually a partial, or in the case of four family members (PAX3, PAX4, PAX6 and PAX7), a complete homeodomain to the C-terminus. (wikipedia.org)
  • Alveolar RMS can be characterized by a recurrent cytogenetic alteration involving FOXO-1 and PAX3 or PAX7 genes, and the consecutive translocations (t(2;13) or t(1;13) respectively) lead to the excess synthesis of fusion proteins with oncogenic effects [ 5 , 6 ]. (biomedcentral.com)
  • Unstable MyoD oscillations and long periods of sustained MyoD expression are observed when stem cells undergo myogenic differentiation. (bvsalud.org)
  • PAX7 directs postnatal renewal and propagation of myogenic satellite cells but not for the specification. (wikipedia.org)
  • Here, we examined the time-dependent expression patterns of multiple biomarkers associated with satellite cell fate, including the transcription factor paired box 7 (Pax7), myoblast determination protein (MyoD), myogenin, and insulin-like growth factor (IGF-1), using immunohistochemistry, western blotting, and quantitative real-time PCR in contused skeletal muscle. (bvsalud.org)
  • We demonstrate enrichment of RUNT family transcription-factor-binding motifs in human epidermal CD8+CD103+CD49a+ TRM cells, paralleled by high RUNX2 and RUNX3 protein expression. (scilifelab.se)
  • It is concluded that MYOD acts as a transcription factor to induce MEG3 expression. (bvsalud.org)
  • The bHLH transcription factor MyoD is a master regulator of myogenic differentiation, and its sustained expression in fibroblasts suffices to differentiate them into muscle cells. (bvsalud.org)
  • The oscillatory expression of MyoD is driven by the oscillatory expression of the bHLH transcription factor Hes1 that periodically represses MyoD. (bvsalud.org)
  • Here, we describe time-lapse imaging methods using luciferase reporters, which can monitor dynamic MyoD gene expression in myogenic cells. (bvsalud.org)
  • Pax7, MyoD, and myogenin protein expression levels confirmed the results of the morphometrical analysis. (bvsalud.org)
  • The control of MuSC state depends on the expression levels of myogenic transcription factors. (biomed.news)
  • Despite its cellular simplicity (five pairs of neurons), the MG exhibits conservation of transcription factor expression with the spinal cord of vertebrates. (biologists.com)
  • Furthermore, we have successfully predicted and validated that the transcription factor myogenic differentiation (MYOD) binds to the MEG3 core promoter though utilizing chromatin immunoprecipitation (CHIP) and luciferase reporter assays. (bvsalud.org)
  • The results indicated that MYOD acts as a transcription factor of MEG3 to promote MEG3 transcription. (bvsalud.org)
  • It is concluded that MYOD acts as a transcription factor to induce MEG3 expression. (bvsalud.org)
  • The bHLH transcription factor MyoD is a master regulator of myogenic differentiation, and its sustained expression in fibroblasts suffices to differentiate them into muscle cells. (bvsalud.org)
  • Unstable MyoD oscillations and long periods of sustained MyoD expression are observed when stem cells undergo myogenic differentiation. (bvsalud.org)
  • The oscillatory expression of MyoD is driven by the oscillatory expression of the bHLH transcription factor Hes1 that periodically represses MyoD. (bvsalud.org)
  • Here, we describe time-lapse imaging methods using luciferase reporters, which can monitor dynamic MyoD gene expression in myogenic cells. (bvsalud.org)
  • Here, we examined the time-dependent expression patterns of multiple biomarkers associated with satellite cell fate, including the transcription factor paired box 7 (Pax7), myoblast determination protein (MyoD), myogenin, and insulin-like growth factor (IGF-1), using immunohistochemistry, western blotting, and quantitative real-time PCR in contused skeletal muscle. (bvsalud.org)
  • Morphometrically, the data obtained from the numbers of Pax7 + , MyoD + , and myogenin + cells were highly correlated with the wound age. (bvsalud.org)
  • Pax7, MyoD, myogenin, and IGF-1 expression patterns were upregulated after injury at both the mRNA and protein levels. (bvsalud.org)
  • Pax7, MyoD, and myogenin protein expression levels confirmed the results of the morphometrical analysis. (bvsalud.org)
  • Expression of the myogenic regulatory factors required to promote differentiation, MYOD and MYOG, was downregulated in the absence of JARID2, even though decreases in the methylation of histone H3 lysine 27 (H3K27 me3 ) were observed on both promoters. (biomedcentral.com)
  • Skeletal muscle differentiation is controlled by four highly related basic helix-loop-helix proteins known as the myogenic regulatory factors (MRFs), which include MYF5, MYOD, MYOG and MYF6. (biomedcentral.com)
  • Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). (novusbio.com)
  • In addition to activating muscle specific genes, members of the MyoD family members activate their own transcription and transactivate the transcription of other MyoD family members. (novusbio.com)
  • Pax4 undergoes mutual reciprocal interaction with the transcription factor Arx to endow pancreatic endocrine cells with insulin and glucagon cells respectively PAX5 has been identified with neural and spermatogenesis development and b-cell differentiation. (wikipedia.org)
  • Restoration of functional PAX3 transcriptional factor enhanced neuronal differentiation in PAX3b isoform-depleted neuroblastoma cells. (nih.gov)
  • The methylation status of CpG islands and surrounding regions (CpG island shores) is an important factor in regulating chromatin structure and gene expression during development and differentiation (Deaton and Bird 2011 ). (springer.com)
  • The Sox6 transcription factor is a key regulator of fast-twitch muscle fiber differentiation in the zebrafish, a role similar to that ascribed to its murine ortholog. (biomedcentral.com)
  • Krüppel-like factor 4 (KLF4) is an evolutionarily conserved zinc finger-containing transcription factor that regulates diverse cellular processes such as cell proliferation, apoptosis, and differentiation. (biomed.news)
  • 6. A Pax3/Dmrt2/Myf5 regulatory cascade functions at the onset of myogenesis. (nih.gov)
  • 16. Direct molecular regulation of the myogenic determination gene Myf5 by Pax3, with modulation by Six1/4 factors, is exemplified by the -111 kb-Myf5 enhancer. (nih.gov)
  • 15] This results in increased PAX activity, leading to dedifferentiation and the proliferation of myogenic cells. (medscape.com)
  • Transfection of myogenin and other family members into a variety of non-muscle cells has been shown to either convert these cells to myogenic cells, or to transcriptionally activate a set of otherwise unexpressed muscle-specific genes. (novusbio.com)
  • 13. Controlling long-range genomic interactions at a native locus by targeted tethering of a looping factor. (nih.gov)
  • A translocation involving the PAX3 locus, between the long arm of chromosome 2 and the long arm of chromosome 13, has been identified in RMS patients. (medscape.com)
  • 15. Endothelial cell specification in the somite is compromised in Pax3-positive progenitors of Foxc1/2 conditional mutants, with loss of forelimb myogenesis. (nih.gov)
  • 9. FACS-Seq analysis of Pax3-derived cells identifies non-myogenic lineages in the embryonic forelimb. (nih.gov)
  • PAX7 directs postnatal renewal and propagation of myogenic satellite cells but not for the specification. (wikipedia.org)
  • This leads to disassembly of the destruction complex releasing free β-catenin in the cytoplasm, which later translocates to the nucleus and activates gene expression in concert with the T cell factor (TCF) and lymphoid enhancer-binding factor (LEF) families of transcription factors. (biomedcentral.com)
  • The transcription factor Sox6 has been implicated in regulating muscle fiber type-specific gene expression in mammals. (biomedcentral.com)
  • 12. Expression patterns of FSHD-causing DUX4 and myogenic transcription factors PAX3 and PAX7 are spatially distinct in differentiating human stem cell cultures. (nih.gov)
  • Downregulation of the paired box gene 3 inhibits the progression of skin cutaneous melanoma by inhibiting c-MET tyrosine kinase : PAX3 downregulation inhibits melanoma progression. (nih.gov)
  • Originally discovered as axon guidance factors, they have been implicated in cancer progression, embryonal organogenesis, skeletal muscle innervation, and other physiological and developmental functions in different tissues. (biomed.news)
  • 2. Time-dependent Pax3-mediated chromatin remodeling and cooperation with Six4 and Tead2 specify the skeletal myogenic lineage in developing mesoderm. (nih.gov)
  • 11. Role of LDB1 in the transition from chromatin looping to transcription activation. (nih.gov)
  • 1. Pax3 cooperates with Ldb1 to direct local chromosome architecture during myogenic lineage specification. (nih.gov)
  • 19. The Co-operation of RUNX1 with LDB1, CDK9 and BRD4 Drives Transcription Factor Complex Relocation During Haematopoietic Specification. (nih.gov)
  • In zebrafish, loss of function of the transcription factor Prdm1a results in a slow to fast-twitch fiber type transformation presaged by ectopic expression of sox6 in slow-twitch progenitors. (biomedcentral.com)
  • 16] PAX3-FOXO fusion is more common than PAX7-FOXO fusion (55% vs 23%) and is associated with worse overall survival. (medscape.com)
  • The translocation t(2;13)(q35;q14), which represents a fusion between PAX3 and the forkhead gene, is a frequent finding in alveolar rhabdomyosarcoma. (nih.gov)