• Activated satellite cells initially proliferate as skeletal myoblasts before undergoing myogenic differentiation. (wikipedia.org)
  • Broadly, skeletal muscle development results from proliferation and differentiation of myoblasts that fuse to form multinucleated myotubes. (poultryhub.org)
  • Ensure high yields when passaging and culturing your primary human skeletal muscle progenitor cells (myoblasts) by using MyoCult™-SF Attachment Substrate, which is optimized to support myoblast attachment. (stemcell.com)
  • Myoblasts cultured using MyoCult™-SF Expansion Medium can be differentiated into multinucleated myotubes using MyoCult™ Differentiation Kit (Human) . (stemcell.com)
  • A C2C12 myoblasts were transfected with Tug1 or control LNAs (25 n M) for 24 h during proliferation (Prolif) or for 1 to 4 days during differentiation (Diff). (biomedcentral.com)
  • After several rounds of proliferation, most SCs differentiate into myoblasts, which fuse to form new myofibers [ 5 ]. (biomedcentral.com)
  • We previously reported that local injection of single, high-dose bFGF into the paralyzed vocal fold 1 month after RLN transection compensated for an atrophied TA muscle by inducing activation of SC proliferation and increasing the number of myoblasts in the RLN paralysis model of rats [ 12 ]. (biomedcentral.com)
  • Our research indicates that intrinsic differences between myoblasts before overt differentiation leads to diversity in muscle fiber types. (rosalindfranklin.edu)
  • These lineages are defined are distinct types of embryonic myoblasts that are committed to the differentiation of diverse, lineage-based, muscle fiber types. (rosalindfranklin.edu)
  • Among these genes is the fibroblast growth factor receptor 1 (FGFR1) gene which is expressed in proliferating myoblasts and down-regulated during differentiation. (rosalindfranklin.edu)
  • The goal of our on-going research is to define and characterize the transcriptional complexes resident on the FGFR1 regulatory regions as well as those interactions off the promoter that govern its expression in both proliferating myoblasts and differentiated muscle fibers. (rosalindfranklin.edu)
  • TNF- TNF- is transiently upregulated in myoblasts inside 3 to 48 h post differentiation induction inside a dose-dependent manner: myogenesis is stimulated at low TNF- concentrations, although is inhibited at higher concentrations [80,81]. (rockinhibitor.com)
  • In addition, this proliferating impact is exerted on satellite cells after in vivo TNF- intraperitoneal injection [82], while Myog is lowered confirming differentiation inhibition of this cytokine on myoblasts [85]. (rockinhibitor.com)
  • IFN- IFN-, a pro-inflammatory cytokine, favors myoblast proliferation, prevents fibrotic events in SkMR, and is expressed by proliferating myoblasts although not by differentiated cells. (rockinhibitor.com)
  • Neither siRNA-mediated Stac3 knockdown nor plasmid-mediated Stac3 overexpression affected the proliferation of C2C12 myoblasts. (vt.edu)
  • Stac3 knockdown promoted the differentiation of C2C12 myoblasts into myotubes as evidenced by increased fusion index, increased number of nuclei per myotube, and increased mRNA and protein expression of myogenic markers including myogenin and myosin heavy chain. (vt.edu)
  • Compared to wild-type myoblasts, myoblasts from Stac3 knockout mouse embryos showed accelerated differentiation into myotubes in culture as evidenced by increased fusion index, increased number of nuclei per myotube, and increased mRNA expression of myogenic markers. (vt.edu)
  • Skeletal muscle progenitors (satellite cells), murine cell line and immortalized human myoblasts are studied in vitro to identify, in a controlled environment, the pathways regulating the regenerative potential of the skeletal muscle. (units.it)
  • Because of the decreased proliferation potential [9] and lower absolute number of satellite cells in muscles from the aged rats compared with adult rats [1,10,11] , one may hypothesize that the aged rats would not yield enough myoblasts to produce myooids in culture. (josam.org)
  • The results obtained showed that senescent myoblasts exhibited an enlarged and flattened morphology, with increased SA-β-gal expression, reduced myogenic differentiation, decreased expression of myogenin, and an increased percentage of cells in the G0/G1 phase. (iium.edu.my)
  • In conclusion, C. vulgaris improves the regenerative capacity of young and senescent myoblasts and promotes myoblast differentiation, indicating its potential to promote muscle regeneration. (iium.edu.my)
  • The transplantation of cultured myoblasts into mature skeletal muscle is the basis for a new therapeutic approach to muscle and non-muscle diseases: myoblast-mediated gene therapy. (rupress.org)
  • Both enriched and clonal populations of primary myoblasts were characterized in assays of cell proliferation and differentiation. (rupress.org)
  • Within five days of transplantation into muscle of mature mice, primary myoblasts had fused with host muscle cells to form hybrid myofibers. (rupress.org)
  • To assess tumorigenicity, primary myoblasts and myoblasts from the C2 myogenic cell line were transplanted into immunodeficient mice. (rupress.org)
  • The ease of isolation, growth, and transfection of primary mouse myoblasts under the conditions described here expand the opportunities to study muscle cell growth and differentiation using myoblasts from normal as well as mutant strains of mice. (rupress.org)
  • The properties of these cells after transplantation--the stability of resulting hybrid myofibers without immune suppression, the persistence of transgene expression, and the lack of tumorigenicity--suggest that studies of cell-mediated gene therapy using primary myoblasts can now be broadly applied to mouse models of human muscle and non-muscle diseases. (rupress.org)
  • Whereas hypertrophy was similar in both TM groups, cell proliferation and Pax7 levels were more robust in the 6-h muscle, mainly posthatch, suggesting a differential effect of various TM periods on cell reservoir vs. hypertrophy and a high sensitivity of myoblasts to relatively small changes in heat duration with respect to these processes, which is manifested in the short and long term. (agri.gov.il)
  • Between E12 and E16, fetal myoblasts fuse with both each other and embryonic myotubes to form fetal myofibers that serve as the foundation for future skeletal muscle. (physiciansontherise.org)
  • We decided E11, E12, E13, and E14 as period points for evaluation to trace advancement right from the start of embryonic myogenesis, when the Pax3+ dermomyotome-derived cells enter the myogenic lineage, towards the onset of fetal myogenesis, when the myoblasts/myotubes begin to type myofibers. (physiciansontherise.org)
  • In this study, we investigate the effects of neural cell integration into the bioprinted skeletal muscle construct to accelerate functional muscle regeneration in vivo. (nature.com)
  • 20% of the original mass invariably results in functional impairment with limited regeneration capacity, which requires reconstructive surgical procedures such as autologous muscle flap transfers 1 . (nature.com)
  • These cells represent the oldest known adult stem cell niche, and are involved in the normal growth of muscle, as well as regeneration following injury or disease. (wikipedia.org)
  • The process of muscle regeneration involves considerable remodeling of extracellular matrix and, where extensive damage occurs, is incomplete. (wikipedia.org)
  • Injection of FGF-6 recombinant protein into damaged mouse muscle can induce cyclin D1 mRNA and differentiation markers, including Cdkl1, MHC I and Tn1, which results in the acceleration of muscle regeneration. (biolegend.com)
  • During muscle regeneration, it has been shown that FGF-6 can be released from necrotic myofibers and be sequestered by basal laminae. (biolegend.com)
  • Satellite cells and skeletal muscle regeneration, Compr. (copernicus.org)
  • Skeletal muscle regeneration is regulated by coordinated activation of multiple signaling pathways. (elifesciences.org)
  • However, the role of individual arms of the UPR in skeletal muscle regeneration remain less understood. (elifesciences.org)
  • Myofiber-specific ablation of IRE1α or XBP1 in mice diminishes skeletal muscle regeneration that is accompanied with reduced number of satellite cells. (elifesciences.org)
  • Finally, targeted ablation of IRE1α also reduces Notch signaling, abundance of satellite cells, and skeletal muscle regeneration in the mdx mice, a model of Duchenne muscular dystrophy. (elifesciences.org)
  • Collectively, our experiments suggest that the IRE1α-mediated signaling promotes muscle regeneration through augmenting the proliferation of satellite cells in a cell non-autonomous manner. (elifesciences.org)
  • The findings shed new light on mechanisms of regeneration of healthy myofibers after severe tissue injury and suggest interplay between these fibers and muscle satellite cells though mechanisms remain to be elucidated. (elifesciences.org)
  • However, the mechanisms of muscle regeneration are not yet fully understood. (elifesciences.org)
  • 1 2 Residing outside the sarcolemma but within the basal lamina of individual myofibers is a population of cells that are responsible for muscle repair and regeneration, the satellite cells. (arvojournals.org)
  • 6 These manipulations result in satellite cell activation and proliferation, which in turn are responsible for muscle repair and regeneration. (arvojournals.org)
  • Therefore, medications that can prevent the progression of muscle atrophy or promote muscle regeneration are in high demand. (biomedcentral.com)
  • Considering that reinnervation of the TA muscle can occur even after UVFP through these anastomoses, it would be ideal if drugs that promote nerve regeneration could be used to treat UVFP. (biomedcentral.com)
  • Satellite cells (SCs), which are precursors of skeletal muscles, such as the TA muscle, play a key role in muscle homeostasis and regeneration. (biomedcentral.com)
  • SCs are normally in a quiescent state in adult muscles, and start proliferating during muscle regeneration in response to denervation. (biomedcentral.com)
  • It is unknown whether the spatiotemporal coupling between myofiber and microvascular regeneration persists when muscle tissue is completely removed and local guidance cues are lost. (biomedcentral.com)
  • To test whether complete removal of skeletal muscle tissue affects the spatiotemporal relationship between myogenesis and angiogenesis during regeneration, subthreshold volumetric muscle loss was created with a biopsy punch (diameter, 2 mm) through the center of the gluteus maximus (GM) in adult mice. (biomedcentral.com)
  • Following punch biopsy of adult skeletal muscle, regenerating microvessels span the wound and become perfused with blood prior to myofiber regeneration. (biomedcentral.com)
  • We conclude that angiogenesis precedes myogenesis during regeneration following subthreshold volumetric muscle loss. (biomedcentral.com)
  • Therefore, as an alternative approach to test the interdependence of microvascular and myofiber regeneration following acute injury, a punch biopsy (diameter, 2 mm) was performed through the mouse gluteus maximus (GM) muscle. (biomedcentral.com)
  • 2008 In addition to satellite cells recent studies have recognized an important part for fibro/adipogenic progenitors (FAPs) in muscle mass regeneration and its fatty degeneration (Joe et al. (scienceexhibitions.org)
  • In particular, her work has been focusing on the molecular mechanisms involved in the modulation of the peripheral synaptic transmission, skeletal muscle adaptation and regeneration in physiological and pathological conditions. (units.it)
  • The research activity of the group is focused on the identification of new strategies to improve skeletal muscle regeneration. (units.it)
  • One of the great advantages of tissue engineering of skeletal muscle is that adult skeletal muscle contains many myogenic precursor stem cells, termed "satellite cells", which are involved in the repair and regeneration of skeletal muscle throughout the lifespan. (josam.org)
  • This study is aimed at determining the effects of C. vulgaris on promoting muscle regeneration by evaluating myoblast regenerative capacity in vitro. (iium.edu.my)
  • Low-intensity pulsed ultrasound (LIPUS, a form of mechanical stimulation) can promote skeletal muscle functional repair, but a lack of mechanistic understanding of its relationship and tissue regeneration limits progress in this field. (ijbs.com)
  • We investigated the hypothesis that specific energy levels of LIPUS mediates skeletal muscle regeneration by modulating the inflammatory microenvironment. (ijbs.com)
  • LIPUS at intensity of 60mW/cm 2 could significantly promoted skeletal muscle regeneration through shifting macrophage phenotype from M1 to M2. (ijbs.com)
  • Lots of the systems that consider recognized place during myogenesis are re-activated during skeletal muscles regeneration in adults, like the activation of skeletal muscle-specific SSTFs13, to be able to translate any insights obtained between systems. (physiciansontherise.org)
  • In addition, the urethral caliber, speed of urothelial regeneration, content of smooth muscle, and vessel density were significantly improved in the group with autologous USC-seeded SIS. (biomedcentral.com)
  • Dumont's research program is divided into 4 axes: 1) characterizing the intrinsic mechanisms regulating the cell fate decision of muscle stem cells during myogenesis, 2) characterizing the interactions between muscle stem cells and their microenvironment (e.g., macrophages, fibro-adipogenic progenitors, vascular cells, fibrosis, hyperoxia) in muscle regeneration and diseases, 3) characterizing the impact of genetic myopathies (e.g. (chusj.org)
  • For example, NF-a modulates myogenesis by affecting directly myoblast cell Proliferation/differentiation or induction of apoptosis. (cdc.gov)
  • We investigated the effects of TNF-alpha on transcriptional changes occurring during myoblast differentiation in C2C 12 cells, a well-characterized in vitro model of myogenesis. (cdc.gov)
  • This study supports the hypothesis that a complex regulatory role of TNF-alpha exits in skeletal myogenesis. (cdc.gov)
  • MicroRNA-1/206(miRNA-1/206) is specifically expressed in skeletal muscle and play a critical role in myogenesis. (biomedcentral.com)
  • Mitochondria and myogenesis-related gene and protein levels in response to Tug1 knockdown during myocyte differentiation, in vitro. (biomedcentral.com)
  • During vertebrate myogenesis, myoblast cell populations proliferate and fuse to form multinucleated muscle fibers that express a battery of contractile protein genes. (rosalindfranklin.edu)
  • Activation of IL-4/IL-13 signaling promotes proliferation of FAPs to support myogenesis while inhibiting their differentiation into adipocytes. (scienceexhibitions.org)
  • Since all known forelimb skeletal muscle tissues are based on Pax3+ progenitor cells, the lineage offers a genetic tool to discover the molecular processes that determine forelimb organogenesis and myogenesis. (physiciansontherise.org)
  • We will perform single cell RNA sequencing (scRNA-seq) of embryonic, fetal, juvenile and adult muscle stem cells (MuSCs) and identify unique gene signatures and cell states across human myogenesis. (ca.gov)
  • Clearance of defective muscle stem cells by senolytics restores myogenesis in myotonic dystrophy type 1 (2022). (chusj.org)
  • Senolytics target defective muscle stem cells and restore myogenesis in myotonic dystrophy type 1. (chusj.org)
  • Neural input into this bioprinted skeletal muscle construct shows the improvement of myofiber formation, long-term survival, and neuromuscular junction formation in vitro. (nature.com)
  • These approaches have achieved bioengineered skeletal muscle tissue fabrication with cellular alignment in vitro, and some showed a degree of therapeutic potential in vivo. (nature.com)
  • 10 Even more compelling is a recent study that subjected individual quiescent satellite cells isolated from adult limb muscle to stretching in vitro, and showed that direct stretching of the satellite cells themselves results in activation and bromodeoxyuridine (BrdU) incorporation. (arvojournals.org)
  • The bacteria-produced GM-CSF stimulated the maturation of bone marrow-derived dendritic cells (BMDCs), while IL-7 promoted the proliferation of spleen isolated T cells and inhibited cytotoxicity T cell apoptosis in vitro. (bvsalud.org)
  • Direct electrical stimulation of muscle fibers in vitro is also being employed in our studies. (rosalindfranklin.edu)
  • Satellite cells are in general easy to isolate from explanted skeletal muscle, and presumably muscle biopsies from an adult mammal would provide an ample supply of cells committed to the myogenic developmental pathway to facilitate the in vitro engineering of functional skeletal muscle. (josam.org)
  • To study the capacity of satellite cells to form into functional skeletal muscle tissue de novo in culture, we have developed several in vitro tissue models. (josam.org)
  • Myooids were activated by transverse electrical fields in a manner similar to that used to activate whole skeletal muscles in vitro during standard physiological measures of muscle function [8] . (josam.org)
  • Then, we comprehensively assessed the functional and histological parameters of skeletal muscle injury in mice and the phenotype shifting in macrophages through molecular biological methods and immunofluorescence analysis both in vivo and in vitro . (ijbs.com)
  • Satellite cells are precursors to skeletal muscle cells, able to give rise to satellite cells or differentiated skeletal muscle cells. (wikipedia.org)
  • Abstract Background Rhabdomyosarcoma (RMS) is a pediatric soft tissue sarcoma arising from myogenic precursors that have lost their capability to differentiate into skeletal muscle. (figshare.com)
  • Furthermore, the stage with the muscle healing process influences the effects of macrophages on myogenic precursors. (rockinhibitor.com)
  • Macrophages expressing pro-inflammatory markers are abundant in regenerating regions damaging for Myog (a transcription aspect expressed only in differentiated myogenic cells) suggesting various associations depending on proliferation or differentiation of myogenic precursors [78,79]. (rockinhibitor.com)
  • 6. Cytokines and Muscle Healing Cytokines are also involved Ziritaxestat MedChemExpress within the complicated crosstalk among myogenic precursors and macrophages, as described below and summarized in Tables 4 and five, and Figure five).Int. J. Mol. (rockinhibitor.com)
  • During preimplantation stages, differentiation occurs between precursors of embryonic and extraembryonic structures. (medscape.com)
  • These dividing cells are known as the "transit amplifying pool" before undergoing myogenic differentiation to form new (post-mitotic) myotubes. (wikipedia.org)
  • After several cell divisions, the satellite cells begin to fuse with the damaged myotubes and undergo further differentiations and maturation, with peripheral nuclei as in hallmark. (wikipedia.org)
  • 2012 whereas paracrine actions of IL-6 and insulin-like growth factors (IGFs) have been implicated in the differentiation of MPs into mature myotubes (Bodine et al. (scienceexhibitions.org)
  • Greater activation also results in increased expression of myogenic basic helix-loop-helix transcription factors MyoD, myogenin, and MRF4 - all responsible for the induction of myocyte-specific genes. (wikipedia.org)
  • All superior and inferior rectus muscles from both globes were examined for BrdU incorporation, MyoD expression, neonatal and developmental myosin heavy chain (MyHC) isoform expression, and myofiber cross-sectional area alterations. (arvojournals.org)
  • In the resected muscle and in the passively stretched antagonist muscle, there was a dramatic increase in the number of myofibers positive for neonatal MyHC and in the number of BrdU- and MyoD-positive satellite cells. (arvojournals.org)
  • Their fate is regulated by the expression of various transcription factors, including paired box 7 (Pax7) and myogenic differentiation (MyoD) [ 6 ]. (biomedcentral.com)
  • In TNF- receptor double-knockout mice, p38 MAPK expression diminishes collectively with MyoD-1, a proliferation marker, in TNF- deficient mice [84]. (rockinhibitor.com)
  • TNF- could possibly be also involved in muscle strength recovery, most likely via modulation of muscle regulatory gene expression, including MyoD [80,84]. (rockinhibitor.com)
  • The miRNA-mRNA profiles and bioinformatics study suggested that the SFRP1 gene was potentially regulated by miRNA-1/206 during porcine skeletal muscle development. (biomedcentral.com)
  • The expression level of the SFRP1 was highly varied across numerous pig tissues and it was down-regulated during porcine skeletal muscle development. (biomedcentral.com)
  • FGF-6 regulates muscle differentiation through a calcineurin-dependent pathway and regulates myofiber size through IGF-II/TGF2R pathway. (biolegend.com)
  • The polycomb-group protein EZH2 is a Lys27 histone H3 methyltransferase that regulates the balance between cell proliferation and differentiation by epigenetically silencing muscle-specific genes. (figshare.com)
  • 2008 For instance autocrine Notch signaling regulates the activation and proliferation of satellite cells (Bjornson et al. (scienceexhibitions.org)
  • Both active and passive stretch of the rectus muscles produced by strabismus surgery dramatically upregulated the processes of satellite cell activation, integration of new myonuclei into existing myofibers, and concomitant upregulation of immature myosin heavy chain isoforms. (arvojournals.org)
  • This signaling culminates in regulation of transcription factors governing expression of the slow myosin heavy chain 2 gene - indicative of the slow muscle fiber phenotype. (rosalindfranklin.edu)
  • Skeletal muscle myosin heavy chain (MHC) content was measured and MHC isoforms were separated on SDS-PAGE gels and quantified. (josam.org)
  • Myosatellite cells, also known as satellite cells, muscle stem cells or MuSCs, are small multipotent cells with very little cytoplasm found in mature muscle. (wikipedia.org)
  • FGF-6 can stimulate migration of myogenic stem cells and also control the maintenance of muscle progenitor cells. (biolegend.com)
  • Meat science and muscle biology symposium: stem cell niche and postnatal muscle growth, J. Anim. (copernicus.org)
  • Skeletal muscle, the most abundant tissue of the body, has remarkable regenerative capacity mainly due to its resident muscle stem cells, also known as satellite cells. (elifesciences.org)
  • Serra M, Brito C and Alves PM. "Bioengineering strategies for stem cell expansion and differentiation. (sciepub.com)
  • Intro The regenerative response of skeletal muscle mass to ICAM4 injury is dependent within the quiescent populace of skeletal muscle mass stem cells termed the satellite cells which reside beneath the basal lamina of each myofiber (Brack and Rando 2012 Wang and Rudnicki 2012 Upon injury these quiescent satellite cells become triggered and undergo proliferation providing rise to myogenic progenitors (MPs) that ultimately differentiate into mature myofibers. (scienceexhibitions.org)
  • We are developing a target product profile for human satellite cells derived from human pluripotent stem cells which will be used to use muscle stem cells in personalized medicine for patients with devastating muscle wasting or degenerative diseases. (ca.gov)
  • We will molecularly and functionally define muscle stem cells in human muscle in development, juvenile and adult and develop strategies to generate the most regenerative muscle stem cells from hPSCs. (ca.gov)
  • The research in this propoal will benefit CA residents tremendously by understanding how to generate a muscle stem cell capable of responding to injury and undergoing long term repopulation after injury to generate new muscle. (ca.gov)
  • A Human Skeletal Muscle Atlas Identifies the Trajectories of Stem and Progenitor Cells across Development and from Human Pluripotent Stem Cells. (ca.gov)
  • Duchenne muscular dystrophy, Myotonic Dystrophy type 1) and acquired myopathies (e.g., prematurity) on muscle stem cell function, and 4) investigating new therapeutic avenues targeting defective muscle stem cells in various muscular dystrophies. (chusj.org)
  • He did his post-doctoral training at the Ottawa Hospital Research Institute in Dr. Michael Rudnicki's lab, where he studied muscle stem cell defects in Duchenne Muscular Dystrophy. (chusj.org)
  • His research program aims to determine how muscle stem cell function is affected in different muscular disorders with the goal of identifying novel therapeutic avenues. (chusj.org)
  • Targeting muscle stem cells with bioactive lipids for the treatment of muscular dystrophies. (chusj.org)
  • In this paper, we discuss the current available information about the role that different phenotypes of macrophages plays after injury and during the remodelling phase in different tissue types, with particular attention to the skeletal muscle. (hindawi.com)
  • Muscle injury results in quick activation of the innate immune system which exerts pleiotropic effects on regenerating muscle mass (Brunelli and Rovere-Querini 2008 Tidball and Villalta 2010 Within minutes of injury neutrophils infiltrate hurt skeletal muscle mass and launch tissue-damaging reactive molecules which exacerbate muscle mass damage (Tidball 1995 This initial burst of security damage caused by the innate immune system is followed by a wave of reparative macrophages. (scienceexhibitions.org)
  • For instance it has been proposed that classically triggered (M1) macrophages infiltrate early to facilitate the clearance of necrotic debris whereas alternatively triggered (M2) macrophages infiltrate later on to assist with muscle growth (Arnold et al. (scienceexhibitions.org)
  • Depletion of macrophages in vivo eliminated the therapeutic effects of LIPUS, indicating that improvement in muscle function was the result of M2-shifted macrophage polarization. (ijbs.com)
  • The functionally undefined Stac3 gene, predicted to encode a SH3 domain- and C1 domain-containing protein, was recently found to be specifically expressed in skeletal muscle and essential to normal skeletal muscle development and contraction. (vt.edu)
  • Satellite cells are able to differentiate and fuse to augment existing muscle fibers and to form new fibers. (wikipedia.org)
  • Activated satellite cells also begin expressing muscle-specific filament proteins such as desmin as they differentiate. (wikipedia.org)
  • Subsequently these differentiate into muscle fibres which then undergo no further cell division and can only be altered through hypertrophy. (poultryhub.org)
  • Autologous USCs were obtained and characterized, and their capacity to differentiate into urothelial cells (UCs) and smooth muscle cells (SMCs) was tested. (biomedcentral.com)
  • More importantly, the bioprinted constructs with neural cell integration facilitate rapid innervation and mature into organized muscle tissue that restores normal muscle weight and function in a rodent model of muscle defect injury. (nature.com)
  • Fibroblasts within the muscle deposit scar tissue, which can impair muscle function, and is a significant part of the pathology of muscular dystrophies. (wikipedia.org)
  • The use of a muscle creatine kinase germline knockout of the gene encoding Ire1/Ern1 shifts the focus from traditional studies of tissue repair toward the regenerating myofibers. (elifesciences.org)
  • Skeletal muscle is a complex tissue composed of thousands of individual multinucleated myofibers. (arvojournals.org)
  • Furthermore, by conjoint analysis of longissimus dorsi data (tissue-specific expression, muscle module and DE-lncRNAs) and ChIP-PCR revealed NONSUSG002145.1 (adjusted p -values = 0.044), which is coexpressed with the IGF2 gene and binding with ZBED6, may play important roles in ZBED6 KO pig skeletal muscle development. (biomedcentral.com)
  • These findings indicate that the identified lncRNAs may play essential roles in tissue function and regulate the mechanism of ZBED6 action in skeletal muscle development in pigs. (biomedcentral.com)
  • In contrast to preserving residual guidance cues following myotoxins or physical trauma, volumetric muscle loss (VML) removes all tissue components. (biomedcentral.com)
  • To achieve this we firstly established a mouse BAT enriched-miRNA profile by comparing miRNAs expressed in mouse BAT, white adipose tissue and skeletal muscle. (biomedcentral.com)
  • 2010 However reflecting their adipogenic potential FAPs can also give rise to ectopic adipocytes that accumulate in degenerating muscle tissue (Uezumi et al. (scienceexhibitions.org)
  • With ageing, skeletal muscle undergoes a severe reduction in tissue mass, leading to a decrease in strength (sarcopenia). (units.it)
  • Prior to tissue engineering of functional skeletal muscle from satellite cells harvested from aged mammals, two key questions must be addressed. (josam.org)
  • The purpose of the current study was to compare the excitability and contractility of myooids engineered from skeletal muscles of 32-month-old rats with that of myooids engineered from adult rat tissue. (josam.org)
  • Proliferation Phase- Rebuilding of the tissue begins with the recruitment of varied cell types. (zennutrients.com)
  • Fibroblastic or myofibroblastic tumors make up the majority of soft tissue tumors of the aerodigestive tract and range from nonneoplastic or benign proliferations (fibromas) to high-grade malignancies (undifferentiated high-grade pleomorphic sarcomas). (basicmedicalkey.com)
  • p38 mitogen-activated protein kinase has been implicated in both skeletal muscle atrophy and hypertrophy. (biomedcentral.com)
  • We previously reported that local administration of bFGF 1 month after recurrent laryngeal nerve (RLN) paralysis compensated for atrophy of the thyroarytenoid muscle. (biomedcentral.com)
  • As increased neuromuscular junctions are expected to maintain myofiber volume, bFGF administration may prevent thyroarytenoid muscle atrophy in the mid to long term. (biomedcentral.com)
  • These treatments provide only static adjustment to the larynx, and their effects may be limited by the remaining hoarseness due to progressive atrophy of the paralyzed thyroarytenoid (TA) muscle. (biomedcentral.com)
  • On the other hand, despite the atrophy and reduced myogenic potential of the EDL muscles in old rats, when an EDL muscle is cross-age transplanted from an old donor rat into a young host rat, the muscle regenerates as much mass and has as high a capacity for force development as a young muscle transplanted into a young host [4] . (josam.org)
  • We previously showed that bioprinted human skeletal muscle constructs were able to form multi-layered bundles with aligned myofibers. (nature.com)
  • Satellite cells proliferate following muscle trauma and form new myofibers through a process similar to fetal muscle development. (wikipedia.org)
  • It has been shown that the expression of FGF-6 can transiently be upregulated by muscle injuries in both fast and slow myofibers. (biolegend.com)
  • The addition of BrdU-positive myonuclei increased from 1 per 1000 myofibers in cross sections of control muscles to 2 to 3 per 100 myofibers in the resected muscles. (arvojournals.org)
  • It is considered postmitotic, in that the myonuclei residing within these myofibers do not divide in adult muscle. (arvojournals.org)
  • 3 When a muscle is injured, this specialized cell population becomes activated, divides, and either repairs damaged fibers or forms new myofibers. (arvojournals.org)
  • Acute injury to skeletal muscle damages myofibers and fragment capillaries, impairing contractile function and local perfusion. (biomedcentral.com)
  • Myofibers and microvessels regenerate from satellite cells and from surviving microvessel fragments, respectively, to restore intact muscle. (biomedcentral.com)
  • Regenerated myofibers and microvessels were disorganized compared to the uninjured muscle. (biomedcentral.com)
  • The success of myoblast transplantation for correction of intrinsic muscle defects depends on the fusion of implanted cells with host myofibers. (rupress.org)
  • We show that incubation at 39.5°C (increase of 1.7°C from normal conditions) from embryonic days 16 to 18 (E16 to E18) for 3 or 6 h daily increased diameter of myofibers as of day 13 of age and enhanced absolute muscle growth relative to controls, until day 35 of age. (agri.gov.il)
  • The aim of this research is to identify and characterize the molecular regulatory circuitry that establishes these myogenic cell lineages and which thereby establish fast versus fast/slow muscle fiber types, independent of innervation, and dependent of cell lineage commitment. (rosalindfranklin.edu)
  • 2010 FAPs which do not arise from your myogenic lineage are bipotential cells capable of providing rise to fibroblasts and adipocytes. (scienceexhibitions.org)
  • We discovered that the lineage harbors several cell populations not previously defined, including cells that may likely populate the immune and hematopoietic systems parallel to the already known skeletal muscle mass, smooth muscle mass, and neuronal systems. (physiciansontherise.org)
  • The myogenic cells of the muscles constitute a separate cell lineage. (medscape.com)
  • For more information on protocols for the derivation and expansion of human skeletal muscle progenitor cells with MyoCult™, please explore the Product Information Sheet . (stemcell.com)
  • 2009 potentially reflecting the reduced secretion of myogenic growth element IGF-1 by these cells (Wynes and Riches 2003 While these studies demonstrate a facilitative part of innate immune cells in muscle mass regrowth after injury a direct molecular link between the innate immune system and muscle mass progenitor biology remains to be founded. (scienceexhibitions.org)
  • We determined the developmental and functional status of both skeletal muscle progenitor cells as well as adult human satellite cells. (ca.gov)
  • There is a growing body of evidence which demonstrates that following ACS, microRNAs might inhibit fibroblast proliferation and scarring, as well as harmful apoptosis of cardiomyocytes, and stimulate fibroblast reprogramming into induced cardiac progenitor cells. (archivesofmedicalscience.com)
  • Several types of adenosine receptors have been detected in developing and differentiated skeletal muscle cells, but little is known about the functional outcome of AR modulation on muscle AChRs. (units.it)
  • Forkhead box O (FoxO) transcription factors and E3 ubiquitin ligases such as Muscle RING finger 1 (MuRF1) are believed to participate in the regulation of skeletal muscle mass. (biomedcentral.com)
  • Myosatellite cells are located between the basement membrane and the sarcolemma of muscle fibers, and can lie in grooves either parallel or transversely to the longitudinal axis of the fibre. (wikipedia.org)
  • Much of our research focuses on the cellular and molecular mechanisms that regulate the development and differentiation of skeletal muscle fibers and their respective phenotypes. (rosalindfranklin.edu)
  • Published results indicate that protein kinase C (PKC) activity in conjunction with innervation-induced activation of cell signaling mediated by the muscarinic acetylcholine receptor, Gaq, and the 1,4,5 inositol triphosphate receptor 1 (IP3R1) differentially regulate fast versus slow muscle fiber type gene expression in innervated muscle fibers. (rosalindfranklin.edu)
  • The close association of FAPs with regenerating muscle mass fibers along with their manifestation of factors that influence myogenic differentiation such as IL-6 and IGF-1 suggests that these stromal cells may perform a supportive part in myogenic differentiation (Joe et BV-6 al. (scienceexhibitions.org)
  • Both of these cell populations have disadvantages: myogenic cell lines are tumorigenic, and primary cultures contain a substantial percentage of non-myogenic cells which will not fuse to host fibers. (rupress.org)
  • We also demonstrated the feasibility of using these 3D bioprinted human skeletal muscle constructs to treat critical-sized muscle defect injuries with structural and functional restoration in a rodent model 2 . (nature.com)
  • We observed a very significant decline in satellite cell activity as our restrictions became more stringent, which was reflected in their reduced myogenic marker gene expression. (poultryhub.org)
  • Gene expression of Hanwoo satellite cell differentiation in longissimus dorsi and semimembranosus, Bmc Genomics, 20, 156, https://doi.org/10.1186/s12864-019-5530-7, 2019. (copernicus.org)
  • Our results indicate that the SFRP1 gene is regulated by miR-1/206 and potentially affects skeletal muscle development. (biomedcentral.com)
  • Additionally, innervation modulates muscle fiber type and associated fiber type specific gene expression. (rosalindfranklin.edu)
  • We are interested in the transcriptional regulation of the FGFR1 gene because its expression is tightly linked to continued myoblast proliferation. (rosalindfranklin.edu)
  • Weighted gene coexpression network analysis (WGCNA) of 1570 DE-lncRNAs between WT and ZBED6 KO pigs was used to define the following six lncRNA modules specific to different tissues: skeletal muscle, heart, lung, spleen, kidney and liver modules. (biomedcentral.com)
  • IFN- stimulation impairs myoblast fusion and differentiation gene expression, most likely by way of inhibition of Myog expression by Class II Main Histocompatibility Complex transactivator (CIITA). (rockinhibitor.com)
  • By watching the gene appearance information of cells over the developmental period course because they migrate in the dermomyotome into forelimb, we are able to recognize the molecular players coincident with muscles stages because they are produced and preserved in coordination with various other cell lineages in the developing limb framework. (physiciansontherise.org)
  • SSTFs integrate external signals during patterning with shifting gene expression networks that coordinate the migration, proliferation, differentiation, and integration of cell types into fully functioning organs and multi-system limb constructions. (physiciansontherise.org)
  • Myofiber-specific ablation of IRE1α dampens Notch signaling and canonical NF-κB pathway in skeletal muscle of adult mice. (elifesciences.org)
  • The insertional 6 mm of the superior rectus muscles of adult rabbits were resected and reattached to the original insertion site. (arvojournals.org)
  • There are a number of factors that initiate myofiber remodeling in adult skeletal muscles. (arvojournals.org)
  • In this project, the group intends to investigate the nature and the role of adenosine and AR signalling pathways on the two isoforms of muscle AChRs: the embryonic (g-AChR) regulating synaptogenesis and muscle development and the adult (e-AChR) with a role in nerve-muscle communication. (units.it)
  • Functional three-dimensional skeletal muscle constructs (myooids) were engineered from myogenic cells harvested from the muscles of adult and aged rats. (josam.org)
  • Adult and aged rat myooids had only ~35-60% of the skeletal muscle MHC content of control skeletal muscle from rats, the remaining MHC content consisting of isoforms found in cultured fibroblasts but not in control skeletal muscle. (josam.org)
  • In addition, myooids expressed only developmental isoforms of skeletal muscle MHC, known to generate less specific force than adult isoforms. (josam.org)
  • Myooids from aged rats generated greater normalized force than myooids from adult rats, but only 1-3% of that for adult skeletal muscle controls. (josam.org)
  • Normalizing for skeletal muscle MHC content and isoform expression predicts myooid forces of ~6-18% of control adult skeletal muscles. (josam.org)
  • The myooids generated specific forces of ~0.1-3% of skeletal muscle from control adult rats. (josam.org)
  • Evaluate myogenic ability and functional differences of embryonic, fetal, juvenile and adult MuSCs in fusion, proliferation, engraftment and repopulation assays in mouse models of muscle disease. (ca.gov)
  • Also, a vascularization strategy using co-culture with endothelial cells (ECs) and fibroblasts improved the survival of the bioengineered skeletal muscle tissues 20 , 21 . (nature.com)
  • In this study, we assembled transcriptomes using RNA-seq data published in previous studies by our group and identified 11,408 known lncRNAs and 2269 potential lncRNAs in seven tissues, heart, longissimus dorsi, gastrocnemius muscle, liver, spleen, lung and kidney, of ZBED6 KO (lean mass model) and WT Bama pigs. (biomedcentral.com)
  • IFN- expression is at basal levels in wholesome muscle tissues, although increases just after injury, peaking at day 5 post-injury corresponding to immune cell and myoblast infiltration. (rockinhibitor.com)
  • Post-hatch muscle hypertrophy, the subject of Dean's work, occurs through the proliferation and differentiation of myogenic satellite cells into muscle myofibres, which then fuse with existing muscle myofibres to increase their size. (poultryhub.org)
  • Irradiation treatment, which eliminates satellite cells from these muscles, prevents the compensatory hypertrophy in these types of experiments. (arvojournals.org)
  • This was indicated by higher DNA incorporation of thymidine and a higher number of cells expressing PCNA in intact muscle, accompanied by higher Pax7 levels, all reflecting a higher number of myogenic cells, and suggesting that the increased hypertrophy can be attributed to a higher reservoir of myogenic progeny cells produced in response to the TM. (agri.gov.il)
  • However, Pax7 is expressed prominently after satellite cell differentiation. (wikipedia.org)
  • We will identify unique cell surface markers on in vivo derived MuSCs that distinguish PAX7-positive cells from other muscle cells and across cell states. (ca.gov)
  • FGF-6 mRNA can also be detected in testis, heart, and skeletal muscle in adults. (biolegend.com)
  • A single nucleotide transition from G to A in intron 3 of IGF2 , a paternally expressed quantitative trait locus (QTL) in pigs, abrogates ZBED6-IGF2 binding and results in 3-fold greater postnatal expression of IGF2 mRNA in skeletal muscle, leading to increased muscle mass and heart size and reduced fat deposition in pigs. (biomedcentral.com)
  • The ability of C. vulgaris to promote myoblast differentiation was analysed through cellular morphology, real-time monitoring, cell proliferation, senescence-associated β-galactosidase (SA-β-gal) expression, myogenic differentiation, myogenin expression, and cell cycle profiling. (iium.edu.my)
  • Treatment with C. vulgaris resulted in decreased SA-β-gal expression and promotion of myogenic differentiation, as observed via an increased fusion index, maturation index, myotube size, and surface area and an increased percentage of cells that stained positive for myogenin. (iium.edu.my)
  • FGF-6 is involved in both proliferation and differentiation of the myogenic lineages. (biolegend.com)
  • More recently, we have initiated studies to investigate the molecular mechanisms that control the development of distinct myogenic cell lineages during embryonic mygenesis. (rosalindfranklin.edu)
  • These constructs (mm 3 -cm 3 scale) consisted of tens to hundreds of long parallel myofiber bundles, containing densely packed, highly viable, and aligned muscle cells. (nature.com)
  • Understanding the effects of strabismus surgery on muscle cell biological reactions and myofiber remodeling may suggest new approaches for improving surgical outcomes. (arvojournals.org)
  • Studies previously conducted show that HS negatively affects the skeletal muscle growth and development by changing its effects on myogenic regulatory factors, insulin growth factor-1, and heat-shock proteins. (frontiersin.org)
  • Our on-going research is focused on the regulatory mechanisms that link innervation, PKC activity, IP3R1 activity, transcriptional regulators, and expression of slow muscle fiber type specific genes. (rosalindfranklin.edu)
  • Nicolas Dumont obtained his PhD at the Université Laval where he studied the regulatory network between inflammatory cells and skeletal muscles. (chusj.org)
  • These results suggest that the 3D bioprinted human neural-skeletal muscle constructs can be rapidly integrated with the host neural network, resulting in accelerated muscle function restoration. (nature.com)
  • Although paraxial mesoderm and lateral plate mesoderm contribute to the musculature that develops in each particular arch, neural crest cells contribute to the skeletal portion of each arch. (medscape.com)
  • All of these prominences are produced by the proliferation of the neural crest cells that migrate into the arches from the neural crest during the fourth week of gestation. (medscape.com)
  • The cells were cultured in media to allow for proliferation for 24 hrs followed by differentiation media for 2, 24 and 48 hrs in the presence or absence of recombinant mouse TNF-alpha. (cdc.gov)
  • Human skeletal myoblast cells were cultured and underwent serial passaging into young and senescent phases and were then treated with C. vulgaris, followed by the induction of differentiation. (iium.edu.my)
  • With this context of injury and repair a number of factors have been recognized that promote proliferation and differentiation of MPs (Kuang et al. (scienceexhibitions.org)
  • Transcriptional profiling of TNF-a-induced effects on skeletal myogenic differentiation. (cdc.gov)
  • Significant loss of muscle mass may occur in cachexia and sarcopenia, which are major causes of mortality and disability. (mdpi.com)
  • Sarcopenia is characterized by the loss of muscle mass, strength, and function with ageing. (iium.edu.my)
  • This work could provide a cell therapy for muscle disorders including muscular dystrophies or sarcopenia. (ca.gov)
  • A large majority of California residents are affected or will be affected with a muscle disease in their lifetime including severe muscular dystrophies, aging related muscle loss called sarcopenia or muscle weakness. (ca.gov)
  • In the present study, we demonstrate that IRE1α (also known as ERN1) and its downstream target, XBP1, are activated in skeletal muscle of mice upon injury. (elifesciences.org)
  • IGF2 mutation knock-in (IGF2 KI) and ZBED6 knockout (ZBED6 KO) lead to changes in IGF2 expression and increase muscle mass in mice and pigs. (biomedcentral.com)
  • Previous studies in mice have been problematic because they have involved transplantation of established myogenic cell lines or primary muscle cultures. (rupress.org)
  • We reported that LIPUS therapy at intensity of 60mW/cm 2 exhibited the most significant differences in functional recovery of contusion-injured muscle in mice. (ijbs.com)
  • Develop transcription factor over-expression in combination with improved directed differentiation strategies to generate MuSCs from hPSCs with genetic and functional signatures of the in vivo MuSCs. (ca.gov)
  • A bioengineered skeletal muscle construct that mimics structural and functional characteristics of native skeletal muscle is a promising therapeutic option to treat extensive muscle defect injuries. (nature.com)
  • The comprehensive functional tests and histological analysis in vivo indirectly and directly proved the effectiveness of LIPUS for muscle recovery. (ijbs.com)
  • The expression level of the SFRP1 was significantly higher in the embryonic skeletal compared with postnatal skeletal muscle, whereas miR-206 showed the inverse pattern of expression. (biomedcentral.com)
  • The main interest of her research activity is the nerve-muscle communication and the skeletal muscle physiology, including muscle plasticity and impact of ageing on the neuromuscular system. (units.it)
  • The results of the proposed research will advance the understanding of important aspects of the neuromuscular physiology such as the modulation of the neuromuscular transmission and the skeletal muscle plasticity. (units.it)
  • Understanding the mechanisms controling BAT differentiation may identify potential therapeutic targets to enhance BAT-controlled metabolism and to increase EE. (biomedcentral.com)
  • Postdoctoral fellows have the opportunity to investigate numerous aspects of the cellular and molecular mechanisms of skeletal and cardiac muscle development. (rosalindfranklin.edu)
  • Musgrove E, Seaman M, Hedley D. "Relationship between cytoplasmic pH and proliferation during exponential growth and cellular quiescence. (sciepub.com)
  • We hypothesize that the remaining force deficit is due to cellular and sub-cellular disorganization-Myooids lack the density and organization of sarcomeric arrays seen in skeletal muscle. (josam.org)
  • We investigated the cellular and molecular bases for the promotion of muscle development and growth by temperature manipulations (TMs) during late-term chick embryogenesis. (agri.gov.il)
  • In addition, IGF-1 expression in skeletal muscle extends the capacity to activate satellite cell proliferation (Charkravarthy, et al. (wikipedia.org)
  • The immediate post-hatch period is the time of maximal myogenic satellite cell activity, therefore optimizing growth conditions during this period is of great importance. (poultryhub.org)
  • These feeding practises during the critical time of maximal satellite cell activity could be having a large impact on muscle mass accretion. (poultryhub.org)
  • A single, early local administration of high-dose bFGF prevented atrophic changes in the thyroarytenoid muscles by activating satellite cell proliferation and reforming neuromuscular junctions. (biomedcentral.com)
  • Even so, this inhibition is reversible as CIITA is swiftly downregulated, and muscle-specific genes upregulated [86,87]. (rockinhibitor.com)
  • FGF-6 exhibits a very restricted expression profile predominantly in the developing muscle in embryos. (biolegend.com)