• Nonetheless, the enhanced myogenesis leads to the formation of an extensive network of contractile myofibers, and this enhanced myogenesis can be conferred upon myogenic cells from clonal populations, such as G8 or C2C12 cells, if they are co-cultured with the limb mesenchymal cells. (biologists.com)
  • Alter J, Bengal E. (2011) Stress-Induced C/EBP Homology Protein (CHOP) Represses MyoD Transcription to Delay Myoblast Differentiation . (technion.ac.il)
  • Tamir, Y., and Bengal, E. (1998) p53 protein is activated during muscle differentiation and participates with MyoD in the transcription of muscle creatine kinase gene . (technion.ac.il)
  • We have isolated a murine cDNA encoding a 9-kD protein, Chisel (Csl), in a screen for transcriptional targets of the cardiac homeodomain factor Nkx2-5. (ox.ac.uk)
  • We are also exploring the mechanisms that control myogenic cell proliferation versus differentiation in both skeletal and cardiac muscle development. (rosalindfranklin.edu)
  • Chemical compounds that modulate atrial and ventricular cell fate could be used to improve subtype-specific differentiation of endogenous or exogenously delivered progenitor cells in order to promote cardiac regeneration. (biomedcentral.com)
  • Transcription factor GATA4-targeted compounds that have previously shown in vivo efficacy in cardiac injury models were tested for stage-specific activation of atrial and ventricular reporter genes in differentiating pluripotent stem cells using a dual reporter assay. (biomedcentral.com)
  • The Y-box protein MSY3/Csda represses myogenin transcription in skeletal muscle by binding a highly conserved cis-acting DNA element located just upstream of the myogenin minimal promoter (myogHCE). (biomedcentral.com)
  • Skeletal muscle and myogenic C2C12 cells were used to study the effects of MSY3 phosphorylation in vivo and in vitro on its sub-cellular localization and activity, by blocking the IGF1/PI3K/Akt pathway, by Akt depletion and over-expression, and by mutating potential MSY3 phosphorylation sites. (biomedcentral.com)
  • Basic helix-loop-helix (bHLH) myogenic regulatory factors coordinate the correct function and organization of many skeletal muscle functions. (biomedcentral.com)
  • Tamir-Livne Y, Mubariki R, Bengal E . (2017) Adhesion molecule Kirrel3/Neph2 is required for the elongated shape of myocytes during skeletal muscle differentiation . (technion.ac.il)
  • Zetser, A., Gredinger, E., and Bengal, E. (1999) p38 mitogen-activated protein kinase promotes skeletal muscle differentiation: participation of the MEF2C transcription factor . (technion.ac.il)
  • Much of our research focuses on the cellular and molecular mechanisms that regulate the development and differentiation of skeletal muscle fibers and their respective phenotypes. (rosalindfranklin.edu)
  • Tamir, Y., and Bengal E . (2000) Phosphoinositide 3-kinase induces the transcriptional activity of MEF2 proteins during muscle differentiation . (technion.ac.il)
  • Mechanistically, ICN1 upregulated expression of SNAIL1, a transcriptional repressor, to increase TPC number in human ERMS and to block muscle differentiation through suppressing MEF2C, a myogenic differentiation transcription factor. (duke.edu)
  • The mechanisms that regulate myoblast cell proliferation versus differentiation involve extracellular signaling via growth factors, their cognate receptors, intracellular signal transduction cascades, and transcriptional regulation of genes associated with cell proliferation. (rosalindfranklin.edu)
  • Our published findings demonstrate that the FGFR1 gene is positively regulated by the Sp1 transcriptional regulator and negatively regulated by the transcription factor E2F4. (rosalindfranklin.edu)
  • MSY3 (MSY4, YB-2, CSDA, dbpA, ZONAB) is a member of the cold shock domain (CSD) family of proteins also known as Y-box proteins, which are evolutionarily conserved proteins that function as transcription factors and regulators of RNA metabolism and protein synthesis. (biomedcentral.com)
  • A hallmark of events observed during segmentation was the induction of multiple transcription factors, including a large group of homeobox proteins in pace with decay of a large fraction of maternal transcripts. (biomedcentral.com)
  • Global gene profiling reveals enrichment for many genes downstream of developmental signaling molecules and self-renewal pathways, such as TGF-β/bone morphogenic protein, Wnt, or fibroblast growth factor, as well as for those that are involved in specification of mesodermal lineages (myocyte enhancer factor 2A, YY1-associated factor 2, and filamin-β). (tau.ac.il)
  • More recently, we have initiated studies to investigate the molecular mechanisms that control the development of distinct myogenic cell lineages during embryonic mygenesis. (rosalindfranklin.edu)
  • These lineages are defined are distinct types of embryonic myoblasts that are committed to the differentiation of diverse, lineage-based, muscle fiber types. (rosalindfranklin.edu)
  • The aim of this research is to identify and characterize the molecular regulatory circuitry that establishes these myogenic cell lineages and which thereby establish fast versus fast/slow muscle fiber types, independent of innervation, and dependent of cell lineage commitment. (rosalindfranklin.edu)
  • Microarray analyses detected a suite of master developmental regulators that control differentiation and maintenance of diverse cell lineages. (biomedcentral.com)
  • The small muscle-specific protein Csl modifies cell shape and promotes myocyte fusion in an insulin-like growth factor 1-dependent manner. (ox.ac.uk)
  • The activities of transcription factors nuclear factor of activated T cells (NFAT) and myocyte enhancer-binding factor (MEF)2, were also enhanced in an IGF-1 signaling-dependent manner. (ox.ac.uk)
  • Of particular importance has been the identification of two families of transcription factors: the myogenic regulatory factors (MRFs) and the myocyte enhancer factors (MEFs). (biologists.com)
  • Alter, J., Rozentzweig, D., and Bengal, E. (2008) Inhibition of myoblast differentiation by tumor necrosis factor alpha is mediated by c-Jun N-terminal kinase 1 and leukemia inhibitory factor . (technion.ac.il)
  • In C2C12 myogenic cells, blocking the IGF1/PI3K/Akt pathway using LY294002 inhibitor reduced MSY3 phosphorylation levels resulting in its accumulation in the nuclei. (biomedcentral.com)
  • The bHLH transcription factor TWIST1 plays a key role in the embryonic development and in tumorigenesis. (biomedcentral.com)
  • TWIST1 is a transcription factor belonging to the basic helix-loop-helix (bHLH) superfamily. (biomedcentral.com)
  • The dynamic cytoskeletal localization of Csl and its dominant effects on cell shape and behavior and transcription factor activity suggest that Csl plays a role in the regulatory network through which muscle cells coordinate their structural and functional states during growth, adaptation, and repair. (ox.ac.uk)
  • Knocking down Akt expression increased the amount of dephosphorylated MSY3 and reduced myogenin expression and muscle differentiation. (biomedcentral.com)
  • While Akt over-expression rescued myogenin expression in MSY3 overexpressing myogenic cells, ablation of the Akt substrate, (Ser126 located in the MSY3 cold shock domain) promoted MSY3 accumulation in the nucleus and abolished this rescue. (biomedcentral.com)
  • This study highlights a previously undescribed Akt-mediated signaling pathway involved in the repression of myogenin expression in myogenic cells and in mature muscle. (biomedcentral.com)
  • Our data implicate the NOTCH1/SNAI1/MEF2C signaling axis as a major determinant of TPC self-renewal and differentiation in ERMS, raising hope of therapeutically targeting this pathway in the future. (duke.edu)
  • Through fluorescence-activated cell sorting, microarray analysis, in vitro differentiation assays, mixed lymphocyte reaction, and a model of ischemic kidney injury, this study sought to identify and characterize multipotent organ stem/progenitor cells in the adult kidney. (tau.ac.il)
  • Specifically,treatment of primary limb bud cultures with p38 inhibitors increases the expression of myogenic markers and causes a striking increase in formation of myotubes, which were detected using antibodies specific for myosin heavy chain. (biologists.com)
  • This signaling culminates in regulation of transcription factors governing expression of the slow myosin heavy chain 2 gene - indicative of the slow muscle fiber phenotype. (rosalindfranklin.edu)
  • The embryonic AMPs are at the origin of all adult fly muscles and, as we demonstrate here, they express repressors of myogenic differentiation and targets of the Notch pathway known to be involved in muscle cell stemness. (biologists.com)
  • Thus, acetylation of histones is known to increase the expression of genes through transcription activation. (wikipedia.org)
  • This indicated the involvement of epigenetic modulators in the regulation of GATA-dependent transcription. (biomedcentral.com)
  • Among these genes is the fibroblast growth factor receptor 1 (FGFR1) gene which is expressed in proliferating myoblasts and down-regulated during differentiation. (rosalindfranklin.edu)
  • ICN1 expanded TPCs by enabling the de-differentiation of zebrafish ERMS cells into self-renewing myf5+ TPCs, breaking the rigid differentiation hierarchies reported in normal muscle. (duke.edu)
  • Thus, small molecules capable of influencing the fate decisions and differentiation programs of multipotent progenitor cells could facilitate therapeutic regeneration of lost myocardium. (biomedcentral.com)
  • As a consequence, the condensed chromatin is transformed into a more relaxed structure that is associated with greater levels of gene transcription. (wikipedia.org)
  • Csl-expressing myoblasts differentiated and fused normally, although in the presence of insulin-like growth factor (IGF)-1 they showed dramatically enhanced fusion, leading to formation of large dysmorphogenic "myosacs. (ox.ac.uk)