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  • cardiac
  • Experimental studies suggest that cardiac transfer of stem and progenitor cells can have a favorable impact on tissue perfusion and contractile performance of the injured heart. (ahajournals.org)
  • In addition, FGF-5 can function as an autocrine/paracrine mechanism of cardiac cell growth and as a cytoprotective mechanism against irreversible ischemic damage. (ahajournals.org)
  • Although bone marrow SP cells are known to be long-term repopulating hematopoietic stem cells, there is little information about the characteristics of cardiac SP cells (CSPs). (rupress.org)
  • These results suggest that CSPs are intrinsic cardiac stem cells and involved in the regeneration of diseased hearts. (rupress.org)
  • While significant progress has been made in the preclinical stages, the clinical application of cardiac cell therapy is limited by technical challenges, including inability to isolate a pure population of cardiac-specific progenitors capable of robust engraftment and regeneration, lack of appropriate pre-clinical animal models, uncertainty about the best mode of delivery, paucity of adequate imaging modalities, and lack of knowledge about the fate of transplanted cells. (pubmedcentralcanada.ca)
  • However, full clinical translation of stem cell-based therapies has been limited by numerous challenges including the proarrhythmic nature of stem cell derived cardiac grafts. (pubmedcentralcanada.ca)
  • dystrophic
  • In particular, molecular-based therapies are common options to be explored in order to exploit beneficial treatments for cachexia, while gene/cell therapies are mostly used in the attempt to induce a substantial improvement of the dystrophic muscular phenotype. (epfl.ch)
  • muscular
  • Additionally, the development of cell-based therapies for muscular dystrophy and other regenerative diseases are also dependent upon these factors. (jove.com)
  • Despite the advances achieved in understanding the molecular biology of muscle cells in the past decades, there is still need for effective treatments of muscular degeneration caused by muscular dystrophies and for counteracting the muscle wasting caused by cachexia or sarcopenia. (epfl.ch)
  • This review focuses on the description of the use of molecular administrations and gene/stem cell therapy to treat muscular degenerations. (epfl.ch)
  • Different known features of various stem cells are compared in this perspective, and the few available examples of their use in animal models of muscular degeneration are reported. (epfl.ch)
  • transient
  • Others have proposed that transient cell retention may be sufficient to promote functional effects, eg, by release of paracrine mediators. (ahajournals.org)
  • self-renewal
  • Although it is still unclear whether these primitive cells fit the precise definition of stem cells, e.g., self-renewal capacity and reconstitutive capability of the total organ, these findings suggest that the heart has intrinsic stemlike cells, which may participate in its regeneration. (rupress.org)
  • stem cell th
  • Although the mechanistic underpinnings of stem cell therapy are still intensely debated, the concept of cell therapy has already been introduced into the clinical setting, where a flurry of small, mostly uncontrolled trials indicate that stem cell therapy may be feasible in patients. (ahajournals.org)
  • The overall clinical experience also suggests that stem cell therapy can be safely performed, if the right cell type is used in the right clinical setting. (ahajournals.org)
  • At the same time, continued basic research to elucidate the underlying mechanism of stem cell therapy is clearly needed. (ahajournals.org)
  • 5,6 Moreover, the mechanistic underpinnings of stem cell therapy appear to be far more complex that previously anticipated. (ahajournals.org)
  • 7-11 Regardless of the mechanisms, there appears to be general agreement that stem cell therapy has the potential to improve perfusion and contractile performance of the injured heart. (ahajournals.org)
  • The latter concept of stimulating the cell cycle, is particularly appealing with the current interest in stem cell therapy for myocardial infarction and heart failure. (ahajournals.org)
  • A major limitation to the use of stem cell therapy, however, is a lack of reliable imaging technologies for long-term monitoring of implanted cells, and for evaluating its effectiveness. (jove.com)
  • Molecular
  • Therefore, multiple efforts have led to the development of different molecular imaging modalities to monitor stem cell grafting with the ultimate aim to accurately evaluate survival, fate and physiology of grafted stem cells and/or their micro-environment. (jove.com)
  • Finally, based on our current knowledge and the rapid advance in stem cell biology, a prediction of clinical translation for cell therapy protocols combined with molecular treatments is discussed. (epfl.ch)
  • The introduction of immunohistochemistry and the discovery of molecular markers of satellite cells then made them accessible for light microscopic studies and a wealth of information is today available. (diva-portal.org)
  • A molecular marker for the myofibre basal lamina or plasma membrane to enhance the reliability of myonuclei and satellite cell identification were to be included. (diva-portal.org)
  • ii) to evaluate which molecular markers which had been described for satellite cell and stem cell identification in different cell states (quiescence, activated or differentiated) are the most useful for studies on human skeletal muscle. (diva-portal.org)
  • biopsies
  • The advance builds on work published in 2015 when researchers at Duke University grew the first functioning human muscle tissue from cells obtained from muscle biopsies. (eurekalert.org)
  • In their previous work, Bursac and his team started with small samples of human cells obtained from muscle biopsies, called "myoblasts," that had already progressed beyond the stem cell stage but hadn't yet become mature muscle fibers. (eurekalert.org)
  • Therefore the aims of the present thesis were i) to develop a highly reliable method using light microscopy for satellite cell identification and quantification in biopsies of human skeletal muscle in normal and pathological conditions. (diva-portal.org)
  • Upon re-examination of biopsies from power-lifters, power-lifters using anabolic substances and untrained subjects it was observed that the new results on satellite cell frequency were significantly different from those obtained when using staining for NCAM and nuclei alone. (diva-portal.org)
  • mice
  • In summary, we here visually demonstrate the complementarities of BLI, MRI and histology to unravel different stem cell- and/or environment-associated characteristics following stem cell grafting in the CNS of mice. (jove.com)
  • As an example, bone marrow-derived stromal cells, genetically engineered to express the enhanced Green Fluorescent Protein (eGFP) and firefly Luciferase (fLuc), and labeled with blue fluorescent micron-sized iron oxide particles (MPIOs), will be grafted in the CNS of immune-competent mice and outcome will be monitored by BLI, MRI and histology ( Figure 1 ). (jove.com)
  • d , After 5 days of culture in growth medium, integrin-α 7 + CD34 + cells isolated from Myf5-nLacZ Het mice showed β-gal activity. (nih.gov)
  • It reviews previous trials using cell delivery protocols in mice and patients starting with the use of donor myoblasts, outlining the likely causes for their poor results and briefly focusing on satellite cell studies that raise new hope. (epfl.ch)
  • therapies
  • The ability to start from cellular scratch using non-muscle tissue will allow scientists to grow far more muscle cells, provide an easier path to genome editing and cellular therapies, and develop individually tailored models of rare muscle diseases for drug discovery and basic biology studies. (eurekalert.org)
  • Stem cell regenerative therapies hold promise for treating diseases across the spectrum of medicine. (pubmedcentralcanada.ca)
  • endothelial
  • Their myogenic cells and surrounding vascular endothelial cells were Cxcr4- and its ligand Cxcl12-positive, respectively. (nii.ac.jp)
  • Cells negative for blood markers CD45 and CD11b, for endothelial marker CD31 and for mesenchymal marker Sca1 were gated (middle-left panel), and within this population the integrin-α 7 + CD34 + fraction was sorted (right panels). (nih.gov)
  • CSPs in injured heart differentiated into cardiomyocytes, endothelial cells, or smooth muscle cells (4.4%, 6.7%, and 29% of total CSP-derived cells, respectively). (rupress.org)